| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| We have previously identified CdGAP ( for Cdc 42 GTPase activating protein ) as a specific GAP for Rac 1 and Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGF 1 increases staining for Rac 1 , but not Cdc42GAP , in association with the formation of prominent leading lamellae without filopodia . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In contrast with Cdc 42 ( for which the GAP domain of p50RhoGAP is 50 fold more efficient than those of p 190 , Bcr , and 3BP 1 ) and with RhoA ( toward which only p50RhoGAP and p 190 displayed high efficiencies ) , the catalytic efficiencies ( Kcat / Km ) of the GAP domains of p50RhoGAP , p 190 , Bcr , and 3BP 1 on Rac 1 are found to be comparable in a range between 0 . 9 and 2 . 6 min 1 microM 1 . ^^^ These results suggest that p50RhoGAP , p 190 , Bcr , and 3BP 1 are all capable of acting as a negative regulator for Rac 1 mediated signaling , and that , although PAK 1 and IQGAP 1 can couple tightly with both Rac 1 and Cdc 42 , PAK 2 is likely to be a specific effector for Rac 1 instead of Cdc42 . . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| The intrinsic rate of GTP hydrolysis by RhoA is relatively slow when compared to other Rho family GTPases such as Cdc 42 or Rac 1 with a rate constant of 0 . 022 min 1 , which can be further stimulated at least 4000 fold by p 190 or p50RhoGAP . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In contrast to Rac 1 and RhoA , nonprenylated Cdc 42 is able to interact with membrane localized GAPs . ( 2 ) Full length p50RhoGAP and p190RhoGAP react less intensely with nonprenylated Rac 1 than with the prenylated protein , whereas no difference was observed in the reaction of isolated GAP domains of either p50RhoGAP or Bcr with the different types of Rac 1 . ( 3 ) Fluoride exerts a significant inhibitory effect only on the interaction of prenylated Rac 1 with the isolated GAP domains of p50RhoGAP or Bcr . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| We present examples for quantitative characterization of ( 1 ) Rac 1 10 GDP interaction , ( 2 ) Cdc 42 interaction with the GTPase binding domain of the Wiskott Aldrich syndrome protein ( three alternative approaches ) , ( 3 ) accelerated nucleotide exchange reaction of RhoA by the catalytic domains of p190RhoGEF , and ( 4 ) intrinsic and stimulated GTP hydrolysis reaction by the catalytic domain of p50RhoGAP . . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In the oligomeric state , Rac 1 became insensitive to the RhoGAP stimulation , albeit maintaining the responsiveness to the guanine nucleotide exchange factor . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In vitro assays demonstrated that the RhoGAP domain of RICH 1 catalyzed GTP hydrolysis on Cdc 42 and Rac 1 , but not on RhoA . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| These observations provide the first demonstration of a direct regulation of RhoGAP activity through a protein protein interaction and suggest a function for intersectin in Rac 1 regulation and actin dynamics . . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| The RhoGAP domain of Gmip stimulates in vitro the GTPase activity of RhoA , but is inactive towards other Rho family proteins such as Rac 1 and Cdc 42 ; it is also specific for RhoA in vivo . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| The RhoGAP domain of p200RhoGAP stimulated the GTPase activities of Rac 1 and RhoA in vitro and in vivo , and the conserved catalytic arginine residue ( Arg 58 ) contributed to the GAP activity . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| The elimination of the Rac 1 GTP bound form from the cell by the introduction of the Y . pseudotuberculosis YopE RhoGAP protein could be bypassed by the overproduction of either PIP5K or Arf 6 , although the degree of bypass was greater for Arf 6 transfectants . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| A GST fusion of the RhoGAP was tested for its specificity on RhoA , Cdc 42 , and Rac 1 . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| In contrast , the entire cellular pool of Rac 1 was inactivated shortly after translocation of YopE RhoGAP . ^^^ Consistent with the observation that YopE inactivation requires membrane localization of Rac 1 , the presence of YopT in the cell interfered with the action of the YopE RhoGAP . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| RICS is mainly expressed in the brain and functions as a RhoGAP protein for Cdc 42 and Rac 1 in vitro . ^^^ |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|