| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.52781105 |
| The supreme catalytic activities of p 190 and p50RhoGAP toward RhoA reside mostly in their lower Km values ( 1 . 79 and 2 . 83 microM , respectively ) which correlate well with their binding affinity for GMP PNP bound RhoA ( 2 . 18 and 2 . 47 microM , respectively ) , in contrast with Bcr and 3BP 1 which interact with the activated RhoA with much higher Km ( 89 microM ) . 0.52781105^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| BPGAP 1 is a novel RhoGAP that co ordinately regulates pseudopodia and cell migration through the interplay of its BNIP 2 and Cdc42GAP homology domains serving as a homophilic / heterophilic interaction device , an enzymic RhoGAP domain that inactivates RhoA and a proline rich region that binds the Src homology 3 domain of cortactin . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| The Cdc42GAP / fetal liver and bone marrow cells showed a 3 fold increase in Cdc 42 activity but normal Rac and RhoA activities , indicating that Cdc42GAP knockout resulted in a gain of Cdc 42 activity in the hematopoietic tissues . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| BNIP Salpha induces cell rounding and apoptosis by displacing p50RhoGAP and facilitating RhoA activation via its unique motifs in the BNIP 2 and Cdc42GAP homology domain . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In contrast with Cdc 42 ( for which the GAP domain of p50RhoGAP is 50 fold more efficient than those of p 190 , Bcr , and 3BP 1 ) and with RhoA ( toward which only p50RhoGAP and p 190 displayed high efficiencies ) , the catalytic efficiencies ( Kcat / Km ) of the GAP domains of p50RhoGAP , p 190 , Bcr , and 3BP 1 on Rac 1 are found to be comparable in a range between 0 . 9 and 2 . 6 min 1 microM 1 . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In contrast to Rac 1 and RhoA , nonprenylated Cdc 42 is able to interact with membrane localized GAPs . ( 2 ) Full length p50RhoGAP and p190RhoGAP react less intensely with nonprenylated Rac 1 than with the prenylated protein , whereas no difference was observed in the reaction of isolated GAP domains of either p50RhoGAP or Bcr with the different types of Rac 1 . ( 3 ) Fluoride exerts a significant inhibitory effect only on the interaction of prenylated Rac 1 with the isolated GAP domains of p50RhoGAP or Bcr . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| We present examples for quantitative characterization of ( 1 ) Rac 1 10 GDP interaction , ( 2 ) Cdc 42 interaction with the GTPase binding domain of the Wiskott Aldrich syndrome protein ( three alternative approaches ) , ( 3 ) accelerated nucleotide exchange reaction of RhoA by the catalytic domains of p190RhoGEF , and ( 4 ) intrinsic and stimulated GTP hydrolysis reaction by the catalytic domain of p50RhoGAP . . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Here we show that treatment of RhoA with CNF 1 inhibits the intrinsic GTPase activity of RhoA and completely blocks GTPase activity stimulated by the Rho GTPase activating protein ( rhoGAP ) . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Here we report the crystal structure of RhoA and rhoGAP complexed with the transition state analogue GDP . ^^^ RhoGAP also appears to function by stabilizing several regions of RhoA that are important in signalling the hydrolysis of GTP . . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In vitro , both the wild type and S36N mutant HA p 190 proteins showed similar GAP activities toward RhoA , but when expressed in NIH 3T3 fibroblasts only wild type p 190 appeared able to function as a RhoGAP . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrated that CNF 2 reduced RhoA GTPase activity in the presence and absence of P 122 ( RhoGAP ) . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In vitro assays demonstrated that the RhoGAP domain of RICH 1 catalyzed GTP hydrolysis on Cdc 42 and Rac 1 , but not on RhoA . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Genome wide analyses of Rho GTPase activating protein ( RhoGAP ) function in Drosophila using RNA interference identified p 190 RhoGAP as essential for axon stability in mushroom body neurons , the olfactory learning and memory center . p 190 inactivation leads to axon branch retraction , a phenotype mimicked by activation of GTPase RhoA and its effector kinase Drok and modulated by the level and phosphorylation of myosin regulatory light chain . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| The RhoGAP domain of Gmip stimulates in vitro the GTPase activity of RhoA , but is inactive towards other Rho family proteins such as Rac 1 and Cdc 42 ; it is also specific for RhoA in vivo . ^^^ These findings suggest that the signalling pathways controlled by two proteins of the Ras superfamily , RhoA and Gem , are linked via the action of the RhoGAP protein Gmip ( Gem interacting protein ) . . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| The RhoGAP domain of p200RhoGAP stimulated the GTPase activities of Rac 1 and RhoA in vitro and in vivo , and the conserved catalytic arginine residue ( Arg 58 ) contributed to the GAP activity . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| As a RhoGAP , MgcRacGAP has been proposed to down regulate RhoA , which is localized to the cleavage furrow and midbody during cytokinesis . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Rnd proteins function as RhoA antagonists by activating p 190 RhoGAP . ^^^ RESULTS : Here we identify p 190 RhoGAP ( p 190 ) , the most abundant GAP for RhoA in cells , as an interactor with Rnd proteins and show that this interaction is mediated by a p 190 region that is distinct from the GAP domain . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| As compared to Rac , other Rho GTPases tested showed reduced ( Cdc 42 ) or no binding ( RhoA , RhoG ) to OCRL 1 RhoGAP . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| To specifically target RhoA , RhoB , or RhoC function , we have generated a set of chimeric molecules by fusing the RhoGAP domain of p 190 , a GTPase activating protein that accelerates the intrinsic GTPase activity of all three Rho GTPases , with the C terminal hypervariable sequences of RhoA , RhoB , or RhoC . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of human KIAA 1391 and mouse Kiaa 1391 genes encoding novel RhoGAP family proteins with RA domain and ANXL repeats . ^^^ RA domain ( codon 198 274 ) , RhoGAP domain ( codon 363 546 ) , and two Annexin like ( ANXL ) repeats ( codon 657 767 and 796 909 ) of human KIAA 1391 protein were conserved in mouse Kiaa 1391 protein . ^^^ This is the first report on the comprehensive characterization of human KIAA 1391 gene and mouse Kiaa 1391 gene , encoding RhoGAP proteins with RA domain and two ANXL repeats . . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| A GST fusion of the RhoGAP was tested for its specificity on RhoA , Cdc 42 , and Rac 1 . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| GH stimulated formation of GTP bound RhoA requires JAK 2 dependent dissociation of RhoA from its negative regulator p 190 RhoGAP . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| When dephosphorylated , p 190 B RhoGAP has been shown to stimulate the activation of RhoA . ^^^ Moreover , overexpression of a catalytically inactive mutant of SHP 2 inhibited p 190 B RhoGAP tyrosyl dephosphorylation , RhoA activity , and myogenesis . ^^^ These observations strongly suggest that SHP 2 dephosphorylates p 190 B RhoGAP , leading to the activation of RhoA . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Analysis of this protein identified two conserved domains , a RhoGAP domain and an RA domain . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| Although the ExoS RhoGAP inactivates Cdc 42 , Rac , and RhoA in vivo , the relationship between ExoS RhoGAP and the eukaryotic regulators of Rho GTPases is not clear . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| In fibroblasts , the Arhgap gene encodes a RhoGAP , which regulates the small G protein designated RhoA . ^^^ Fluoride treatment of fibroblasts inactivates RhoGAP , thereby activating RhoA , which leads to elevation of filamentous actin ( F actin ) . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| DLC 2 encodes a unique RhoGTPase activating protein ( RhoGAP ) specific for small RhoGTPases , RhoA , and Cdc 42 . ^^^ |
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| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| To investigate the biological function of p 190 RhoGAP toward RhoA in cancer cell invasion and metastasis , we generated a chimera made of the RhoGAP domain of p 190 and the C terminus of RhoA ( p 190 RhoA chimera ) , and transfected it into human pancreatic cancer cells , AsPC 1 . ^^^ These data suggest that the inhibitory action of p 190 RhoGAP toward RhoA offers a novel approach to the treatment of invasion and metastasis of cancer cells . . ^^^ |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|