| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Delineation of a limit Cdc 42 GTPase activating protein domain . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Cloning and expression of a human CDC 42 GTPase activating protein reveals a functional SH 3 binding domain . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Our data indicate that the insert region of Cdc42Hs is not essential for its interactions with various target / effector molecules or for interactions with the guanine nucleotide exchange factor , Dbl , or the Cdc 42 GTPase activating protein ( GAP ) . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Biochemical studies of the mechanism of action of the Cdc 42 GTPase activating protein . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| We have previously identified CdGAP ( for Cdc 42 GTPase activating protein ) as a specific GAP for Rac 1 and Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The catalytic efficiencies ( Kcat / Km ) of the GAP domains of Bcr , 3BP 1 , and p 190 on Cdc 42 are found to be 60 , 160 , and over 500 fold less than that of Cdc42GAP , respectively , and the differences are due , to a large part , to differences in Km . ^^^ These quantitative analysis provide insight that Cdc42GAP functions as an effective negative regulator of Cdc 42 by fast , relatively tight binding to the GTP bound Cdc 42 , whereas IQGAP 1 interacts with Cdc 42 as a putative effector with over 10 fold higher affinity than the CRIB domains and GAPs , and suggest that various GAPs and effectors employ distinct mechanism to play roles in Cdc 42 mediated signaling pathways . . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Moreover , similar to the case of Cdc 42 and Cdc42GAP interaction , Cdc 42 GDP interacted with tetrafluoroaluminate and Cdc 42 GTPgammaS ( guanosine 5 ' 3 O ( thio ) triphosphate ) to form a transition state complex of the GTPase activating reaction in which the carboxyl terminal determinant ( s ) of the GTPgammaS bound Cdc 42 plays a critical role . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Structures of Cdc 42 bound to the active and catalytically compromised forms of Cdc42GAP . ^^^ The Rho related small GTP binding protein Cdc 42 has a low intrinsic GTPase activity that is significantly enhanced by its specific GTPase activating protein , Cdc42GAP . ^^^ In this report , we present the tertiary structure for the aluminum fluoride promoted complex between Cdc 42 and a catalytically active domain of Cdc42GAP as well as the complex between Cdc 42 and the catalytically compromised Cdc42GAP ( R305A ) mutant . ^^^ The Cdc42GAP stabilizes both the switch 1 and switch 2 domains of Cdc 42 and contributes a highly conserved arginine ( Arg 305 ) to the active site . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of the Bcl 2 associated protein BNIP 2 by fibroblast growth factor receptor 1 prevents its binding to Cdc42GAP and Cdc 42 . ^^^ BNIP 2 shares a region of homology with the noncatalytic domain of Cdc42GAP , a GTPase activating protein for the small GTP binding molecule , Cdc 42 . ^^^ We show here that BNIP 2 and Cdc42GAP could directly bind to each other and they also compete for the binding to the same target , Cdc 42 . ^^^ In all cases , tyrosine phosphorylation of BNIP 2 severely impaired its association with Cdc42GAP and its induced GTPase activating protein like activity toward Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| In contrast , the BCH domain of Cdc42GAP , although it can bind Cdc 42 , is catalytically inactive . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The cycling of Cdc 42 between its on ( GTP bound ) and off ( GDP bound ) states is essential for its stimulation of cell growth and transformation , with an important aspect of this cycle being the regulation of the GTP hydrolytic activity of Cdc 42 by its GTPase activating protein ( Cdc42GAP ) . ^^^ On the basis of the structural determinations of the Cdc 42 Cdc42GAP complex , as well as the Ras RasGAP complex , it has been proposed that an arginine residue provided by the GAP ( called the `` arginine finger ' ' ) stabilizes charges developing on the guanine nucleotide during the transition state for GTP hydrolysis and is an important contributor to GAP stimulated catalysis . ^^^ In the study presented here , we have tested this idea by examining three residues within the Cdc42GAP , which are missing in the GAP homology domain of the 85 kDa regulatory subunit ( p 85 ) of the PI 3 kinase and are involved in specific interactions with switch domain residues of Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| BNIP 2 induces cell elongation and membrane protrusions by interacting with Cdc 42 via a unique Cdc 42 binding motif within its BNIP 2 and Cdc42GAP homology domain . ^^^ We previously showed that BNIP 2 interacted with Cdc 42 and its cognate inactivator , p50RhoGAP / Cdc42GAP via its BNIP 2 and Cdc42GAP homology ( BCH ) domain , but its cellular and physiological roles still remain unclear . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Here we have disrupted cdc42gap , a ubiquitously expressed negative regulator of Cdc 42 , in mice . ^^^ Cdc42GAP ( / ) embryonic fibroblasts and various organs displayed significantly elevated Cdc 42 activity . ^^^ These results reveal a role of Cdc42GAP in mammalian perinatal growth and implicate the c Jun N terminal kinase mediated apoptosis machinery as a Cdc 42 effector pathway in vivo . . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Genetic deletion of Cdc42GAP reveals a role of Cdc 42 in erythropoiesis and hematopoietic stem / progenitor cell survival , adhesion , and engraftment . ^^^ Here we have examined the hematopoietic properties and the hematopoietic stem / progenitor cell ( HSP ) functions of gene targeted mice carrying null alleles of cdc42gap , a negative regulator of Cdc 42 . ^^^ The Cdc42GAP / fetal liver and bone marrow cells showed a 3 fold increase in Cdc 42 activity but normal Rac and RhoA activities , indicating that Cdc42GAP knockout resulted in a gain of Cdc 42 activity in the hematopoietic tissues . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Indeed we show that one such slow cycling mutant , Cdc 42 [ Y32A ] , which is insensitive to Cdc42GAP but still exhibits a measurable intrinsic GTP hydrolytic activity , gives rise to increased levels of activated Cdc 42 in NIH 3T3 cells . ^^^ The second approach was to determine whether the transforming activity of the fast cycling Cdc 42 [ F28L ] mutant can be reversed by compensating for its accelerated nucleotide exchange reaction through the expression of the GTPase activating protein ( Cdc42GAP ) and the ensuing stimulation of GTP hydrolytic activity . ^^^ We showed that expression of the limit functional domain of Cdc42GAP inhibited Cdc 42 [ F28L ] induced transformation , as well as selectively reversed the transformed phenotypes caused by the hyperactivation of wild type Cdc 42 in cells expressing the oncogenic version of Dbl ( for Diffuse B cell lymphoma ) , a guanine nucleotide exchange factor for Cdc 42 and the related Rac and Rho GTPases . ^^^ Overall , the results reported here establish the requirement for Cdc 42 to cycle between its signaling on and off states in order to positively influence cell growth and highlight how the Cdc42GAP can play an important role in regulating cell proliferation . . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| We report here the crystal structure of Cdc42Hs , with the non hydrolysable GTP analogue GMPPNP , in complex with the GAP domain of p50rhoGAP at 2 . 7A resolution . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| In contrast with Cdc 42 ( for which the GAP domain of p50RhoGAP is 50 fold more efficient than those of p 190 , Bcr , and 3BP 1 ) and with RhoA ( toward which only p50RhoGAP and p 190 displayed high efficiencies ) , the catalytic efficiencies ( Kcat / Km ) of the GAP domains of p50RhoGAP , p 190 , Bcr , and 3BP 1 on Rac 1 are found to be comparable in a range between 0 . 9 and 2 . 6 min 1 microM 1 . ^^^ These results suggest that p50RhoGAP , p 190 , Bcr , and 3BP 1 are all capable of acting as a negative regulator for Rac 1 mediated signaling , and that , although PAK 1 and IQGAP 1 can couple tightly with both Rac 1 and Cdc 42 , PAK 2 is likely to be a specific effector for Rac 1 instead of Cdc42 . . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The intrinsic rate of GTP hydrolysis by RhoA is relatively slow when compared to other Rho family GTPases such as Cdc 42 or Rac 1 with a rate constant of 0 . 022 min 1 , which can be further stimulated at least 4000 fold by p 190 or p50RhoGAP . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The GTPase activity of TC 10 was lower than that of Cdc 42 , and TC 10 possessed a lower affinity for , but greater responsiveness to , the p50Rho GTPase activating protein ( p50RhoGAP ) than did Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the p50RhoGAP specificity for Cdc 42 was lost in the absence of Mg ( 2+ ) cofactor . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| In contrast to Rac 1 and RhoA , nonprenylated Cdc 42 is able to interact with membrane localized GAPs . ( 2 ) Full length p50RhoGAP and p190RhoGAP react less intensely with nonprenylated Rac 1 than with the prenylated protein , whereas no difference was observed in the reaction of isolated GAP domains of either p50RhoGAP or Bcr with the different types of Rac 1 . ( 3 ) Fluoride exerts a significant inhibitory effect only on the interaction of prenylated Rac 1 with the isolated GAP domains of p50RhoGAP or Bcr . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| We present examples for quantitative characterization of ( 1 ) Rac 1 10 GDP interaction , ( 2 ) Cdc 42 interaction with the GTPase binding domain of the Wiskott Aldrich syndrome protein ( three alternative approaches ) , ( 3 ) accelerated nucleotide exchange reaction of RhoA by the catalytic domains of p190RhoGEF , and ( 4 ) intrinsic and stimulated GTP hydrolysis reaction by the catalytic domain of p50RhoGAP . . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| In vitro , rhoGAP has a striking preference for G25K as a substrate , whilst p190GAP has marked preferential activity for rho . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The GAP domain is active on Rho , Rac , and Cdc 42 in vitro but with a clear preference for Rho ; we refer to the molecule as PTPL 1 associated RhoGAP 1 , PARG 1 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Expression of dominant negative mutants of Rac or Cdc 42 , the Rho inhibitory molecule C 3 transferase , or the GTPase activating protein RhoGAP p 190 causes a marked reduction in the number of primary dendrites in nonpyramidal ( multipolar ) neurons and in the number of basal dendrites in neurons with pyramidal morphologies . ^^^ Strikingly , this shift in favor of nonpyramidal morphologies is also inhibited by the expression of dominant negative mutants of Cdc 42 and Rac and by RhoGAP p 190 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| There is a rotation of 20 degrees between the Rho and rhoGAP proteins in this complex when compared with the ground state complex Cdc42Hs . ^^^ GMPPNP / rhoGAP , in which Cdc42Hs is bound to the non hydrolysable GTP analogue GMPPNP . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Regulation of CDC 42 GTPase by proline rich tyrosine kinase 2 interacting with PSGAP , a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| In vitro assays demonstrated that the RhoGAP domain of RICH 1 catalyzed GTP hydrolysis on Cdc 42 and Rac 1 , but not on RhoA . ^^^ Ectopic expression of the RhoGAP domain as well as the full length protein interfered with platelet derived growth factor BB induced membrane ruffling , but not with serum induced stress fiber formation , further emphasizing the notion that , in vivo , RICH 1 is a GAP for Cdc 42 and Rac1 . . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| The RhoGAP domain of Gmip stimulates in vitro the GTPase activity of RhoA , but is inactive towards other Rho family proteins such as Rac 1 and Cdc 42 ; it is also specific for RhoA in vivo . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Proteins containing a RhoGAP ( Rho GTPase activating protein ) domain usually function to catalyze the hydrolysis of GTP that is bound to Rho , Rac and / or Cdc 42 , inactivating these regulators of the actin cytoskeleton . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Although ExoT RhoGAP stimulates actin reorganization through the inactivation of Rho , Rac , and Cdc 42 , the function of the ADP ribosylation domain is unknown . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Considering the pleiotropic cellular functions of Rho GTPases ( Rho , Rac and Cdc 42 ) and their dysregulation in several forms of mental retardation , we have investigated the so far unexplored function of the RhoGAP domain of OCRL 1 . ^^^ As compared to Rac , other Rho GTPases tested showed reduced ( Cdc 42 ) or no binding ( RhoA , RhoG ) to OCRL 1 RhoGAP . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| A GST fusion of the RhoGAP was tested for its specificity on RhoA , Cdc 42 , and Rac 1 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| RhoGAP interacting with CIP 4 homologs 1 ( RICH 1 ) was previously found in a yeast two hybrid screen for proteins interacting with the SH 3 domain of the Cdc 42 interacting protein 4 ( CIP 4 ) . ^^^ RICH 1 was shown to be a RhoGAP for Cdc 42 and Rac . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Although the ExoS RhoGAP inactivates Cdc 42 , Rac , and RhoA in vivo , the relationship between ExoS RhoGAP and the eukaryotic regulators of Rho GTPases is not clear . ^^^ Green fluorescent protein RhoGDI and ExoS RhoGAP cooperatively stimulated actin reorganization and translocation of Cdc 42 from membrane to cytosol , and a RhoGDI mutant , RhoGDI ( I177D ) , that is defective in extracting Rho GTPases off the membrane inhibited the actions of RhoGDI and ExoS RhoGAP on the translocation of Cdc 42 from membrane to cytosol . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| By using a PCR based subtraction hybridization approach to clone cDNAs from ECs undergoing capillary tube formation , we identified a RhoGAP member , p 73 . p 73 displays GTPase activity to Rho but not to Rac or Cdc 42 . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| ARHGAP 10 , a novel RhoGAP at the cross road between ARF 1 and Cdc 42 pathways , regulates Arp2 / 3 complex and actin dynamics on Golgi membranes ] . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| DLC 2 encodes a unique RhoGTPase activating protein ( RhoGAP ) specific for small RhoGTPases , RhoA , and Cdc 42 . ^^^ |
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| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| Using functional and proteomic screens of proteins that regulate the Cdc 42 GTPase , we have identified a network of protein interactions that center around the Cdc 42 RhoGAP Rich 1 and organize apical polarity in MDCK epithelial cells . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| RICS is mainly expressed in the brain and functions as a RhoGAP protein for Cdc 42 and Rac 1 in vitro . ^^^ |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07960 and P60953 |
Pubmed |
SVM Score :0.0 |
| NA |
|