| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.92881095 |
| Thus , while p 37 binds DNA at the primer end and has a specific affinity for pol epsilon , p 40 , which binds ATP , interacts with PCNA and pol delta . 0.92881095^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.93181476 |
| It is hypothesized from these observations that pol epsilon and PCNA have separate but associated functions early in S phase and that pol epsilon participates with PCNA in DNA replication late in S phase . . 0.93181476^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Distinct nuclear and spindle pole body population of cyclin cdc 2 in fission yeast . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| In contrast , induction of wild type Cdc 2 with a mitotic cyclin did not induce mitosis in pole cells . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| We show that Pum inhibits pole cell division by repressing translation of cyclin B messenger RNA . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| However , DNA synthesis by pol epsilon was stimulated up to 10 fold upon addition of the three auxiliary proteins PCNA , RF C and SSB . ^^^ This stimulation of pol epsilon by PCNA / RF C / SSB appears to be the superposition of two events : pol epsilon holoenzyme ( pol epsilon , PCNA , RF C ) synthesized longer products than its pol epsilon core counterpart , but elongated less primers . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri nuclear association in syncytial embryos . ^^^ These transcripts become evenly distributed throughout the oocyte cytoplasm but , in addition , those of cyclin B become concentrated at the posterior pole . ^^^ Examination of the distributions of RNAs transcribed from chimeric cyclin genes indicates that sequences in the 3 ' untranslated region of the larger cyclin B RNA are required both for it to become concentrated at the posterior pole and to direct those transcripts in the body of the syncytial embryo to their peri nuclear localisation . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Yeast Pol epsilon required the presence of a DNA binding protein ( SSB ) whereas human Pol epsilon required the addition of SSB , Activator 1 and proliferating cell nuclear antigen ( PCNA ) for maximal activity . ^^^ Like Pol delta , Pol epsilon formed complexes with SSB coated primed DNA templates in the presence of Activator 1 and PCNA which could be isolated by filtration through Bio Gel A 5m columns . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Exonuclease containing calf thymus DNA polymerase delta , which requires proliferating cell nuclear antigen for efficient synthesis , is significantly less accurate than pol epsilon . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that two independent mechanisms act on maternally derived cyclin B transcripts to concentrate the transcripts at the posterior pole of the Drosophila oocyte and at the cortex of the syncytial embryo . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| This is suggested by our finding that a specific class of cyclin mRNAs , the products of the cyclin B gene , accumulate in pole cells during embryogenesis ( Whitfield et al . 1989 ) . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Transcripts of one of two Drosophila cyclin genes become localized in pole cells during embryogenesis . ^^^ We show that both genes encode abundant maternal mRNAs , but whereas the cyclin A mRNA is relatively uniformly distributed before cell formation , the cyclin B mRNA becomes localized to the developing pole cells . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Replication factors RF A and RF C , proliferating cell nuclear antigen , and Escherichia coli single stranded DNA binding protein showed no significant effect on this preparation ' s pol epsilon activity , processivity , or substrate specificity . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| To investigate the changes in cell proliferation at 3 different locations ( periphery , equator , posterior pole ) of the sclera , the ratio of positive cells in PCNA , Decorin , b FGF , TGF alpha , TGF beta , IGF 2 , and phosphotyrosine were studied immunohistologically . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| An alternative model is that the p34CDC28 / G1 cyclin complexes have several targets post START , one being DSC 1 and the others being as yet unidentified components of the pathways leading to CDC 4 function , spindle pole body duplication and bud emergence . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Discrete sequence elements control posterior pole accumulation and translational repression of maternal cyclin B RNA in Drosophila . ^^^ The concentration of cyclin B transcripts at the posterior pole of the Drosophila oocyte occurs at a late stage of oogenesis and is dependent on the sequence in the 3 ' untranslated part of the RNA . ^^^ We show that RNA injected into the posterior cytoplasm of syncytial embryos accumulates in the pole cells if it contains sequences present in the 3 ' untranslated region of maternal cyclin B transcripts . ^^^ Zygotic transcription directed from the cyclin B promoter does not begin in the pole cells until the first instar larva has hatched . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Fission yeast microtubule associating protein ( MAP ) p93Dis1 functions for sister chromatid separation : dis 1 mutants fail to separate chromosomes , while the spindle elongates but without cyclin destruction . p93Dis1 localizes along microtubules in interphase cytoplasm , but shifts to the spindle pole body ( SPB ) and spindle microtubules upon the entry into mitosis . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| The importance of the interdomain connector loop and of the carboxy terminal domain of Saccharomyces cerevisiae proliferating cell nuclear antigen ( PCNA ) for functional interaction with DNA polymerases delta ( Poldelta ) and epsilon ( Pol epsilon ) was investigated by site directed mutagenesis . ^^^ Interestingly , the pol 30 79 mutant PCNA ( pcna 79 ) was most defective in replication with Poldelta , whereas pcna 90 was defective in replication with Pol epsilon . ^^^ Protein protein interaction studies showed that pcna 79 and pcna 90 failed to interact with Pol delta and Pol epsilon , respectively . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Both PCNA associated DNA polymerases delta and epsilon are important for gene conversion , though a temperature sensitive Pol epsilon mutant is more severe than one in Pol delta . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| To compare CAG repeats to other simple repeats and palindromic sequences , we examined the effect of DNA replication mutations , including alleles of pol alpha , pol delta , pol epsilon , and PCNA ( proliferating cell nuclear antigen ) , on tract stability . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| We have found that while microtubules are not necessary , the actin cytoskeleton is needed for stable association of nanos , oskar , germ cell less and cyclin B mRNAs and Oskar and Vasa proteins at the posterior pole in the early embryo . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| The genes tagged with jellyfish green fluorescent protein ( GFP ) include sad 1 ( + ) ( encoding a spindle pole body ( SPB ) protein ) , atb 2 ( + ) ( alpha tubulin ) , mis 6 ( + ) ( a kinetochore protein ) , eat 1 ( + ) ( a novel actin like protein localized in the nucleus ) and cdc 13 ( + ) ( a mitotic cyclin ) . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cleavage does not occur during necrosis or following exposure to H ( 2 ) O ( 2 ) or methanesulfonic acid methyl ester . p 140 is unlikely to be able to functionally replace p 261 in vivo , since it does not bind to PCNA or the other pol epsilon subunits . . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin B mRNA was found to be exclusively localized as an aggregation along the cytoplasm at the animal pole of full grown immature oocytes . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Overexpression of cyclin A in human HeLa cells induces detachment of kinetochores and spindle pole / centrosome overproduction . ^^^ In summary , elevated cyclin A levels are important for the capacity of cells to be driven into mitosis by caffeine addition , for the ability of cells to progress to mitosis with detached kinetochores , and for centrosome / spindle pole replication . . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Biochemical characterization of subunit stoichiometry , salt sensitivity , processivity , and stimulation by proliferating cell nuclear antigen indicates that the reconstituted pol epsilon is functionally identical to native pol epsilon purified from yeast and is therefore useful for biochemical characterization of the interactions of pol epsilon with other replication , recombination , and repair proteins . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Proliferating cell nuclear antigen ( PCNA ) has been shown to interact with a variety of DNA polymerases ( pol ) such as pol delta , pol epsilon , pol iota , pol kappa , pol eta , and pol beta . ^^^ Proliferating cell nuclear antigen ( PCNA ) has been shown to interact with a variety of DNA polymerases ( pol ) such as pol delta , pol epsilon , pol iota , pol kappa , pol eta , and pol beta . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Thus , it appears that spindle pole functions of Polo kinase require the degradation of early mitotic targets of the APC / C , such as cyclin A , or other specific proteins . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| Thus , the fidelity of wild type pol delta in the presence of PCNA is more than 2 orders of magnitude lower than the fidelity of wild type pol epsilon holoenzyme and is comparable to the fidelity of exonuclease deficient pol epsilon holoenzyme . . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| In budding yeast cells lacking Asf 1 , the amounts of several DNA replication proteins , including replication factor C ( RFC ) , proliferating cell nuclear antigen ( PCNA ) , and DNA polymerase epsilon ( Pol epsilon ) , are reduced at stalled replication forks . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| In the presence of GINS , Pol epsilon is more processive and dissociates more readily from replicated DNA , while under identical conditions , proliferating cell nuclear antigen slightly stimulates Pol epsilon in vitro . ^^^ |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07864 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|