| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| This study used DNA primer extension and sequencing gel analyses to evaluate the molecular action of 2 ' , 3 ' didehydro 2 ' , 3 ' dideoxythymidine triphosphate ( D4TTP ) , in comparison with 3 ' azido 2 ' , 3 ' dideoxythymidine triphosphate ( AZTTP ) , on DNA strand elongation by human immunodeficiency virus reverse transcriptases ( HIV RT ) and human DNA polymerases alpha ( pol alpha ) and epsilon ( pol epsilon ) purified from T lymphoblastoid CEM cells . ^^^ In contrast , D4TTP was a poor substrate for pol alpha and pol epsilon . ^^^ The analogue was incorporated into DNA by the human enzymes about 10 , 000 to 20 , 000 fold less efficiently than by HIV RT , whereas the incorporation of AZTTP by pol alpha and pol epsilon was not detectable by the DNA primer extension assay . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Their fidelity , in decreasing order , is replication greater than or equal to pol epsilon greater than pol delta greater than pol alpha . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| DNA polymerase epsilon ( pol epsilon ) from HeLa cells was purified to near homogeneity , utilizing Mono S fast protein liquid chromatography for complete separation from pol alpha . ^^^ This non neutralizing polyclonal antiserum specifically recognized the catalytic subunit of pol epsilon by immunoblotting , but not that of pol alpha , beta , or delta . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| DNA polymerase epsilon [ DNA pol epsilon ( EC 2 . 7 . 7 . 7 ) ] is one of the four nuclear DNA polymerases in eukaryotic cells . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| The level of pol epsilon activity tsFT 20 was also significantly lower than in the parental cells , suggesting cooperation and / or interaction between pol alpha and epsilon , and some relevance of pol epsilon to DNA replication . . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Pol epsilon core enzyme was able to fill the gap completely , but like pol alpha , essentially stopped at the pause site . ^^^ Ligation of the DNA products indicated that pol beta core enzyme , pol delta and pol epsilon holoenzymes ( but not pol alpha and pol epsilon core enzyme ) synthesized products that were easily ligatable . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Competitive inhibition studies indicate that like DNA polymerases alpha and delta ( pol alpha and pol delta , respectively ) , free pol epsilon binds single stranded but not double stranded DNA . ^^^ Also like pol alpha and pol beta , pol epsilon exhibits induced dNTP inhibition in the presence of template annealed to complementary primer containing a 2 ' , 3 ' H ( dideoxy ) terminus . ^^^ Enzymologic studies suggest that in contrast to both pol alpha and pol delta , pol epsilon functions more efficiently as gap size decreases . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| The work reported in this article has evaluated the relative molecular activity of the 5 ' triphosphate of a novel beta L nucleoside with an unsaturated ribose residue , beta L 2 ' , 3 ' dideoxy 2 ' , 3 ' didehydro 5 fluorocytidine ( beta L Fd4CTP ) , with that of beta L 2 ' , 3 ' dideoxy 5 fluorocytidine ( beta L FddCTP ) and 2 ' , 3 ' dideoxycytidine ( ddCTP ) , on DNA strand elongation by human immunodeficiency virus 1 reverse transcriptase ( HIV RT ) and human DNA polymerases alpha ( pol alpha ) , beta ( pol beta ) , gamma ( pol gamma ) , and epsilon ( pol epsilon ) . ^^^ The beta L Fd4CTP at a concentration as high as 40 micro M had no inhibitory effect on pol epsilon , but could inhibit pol alpha by 10 20 % at 20 micro M . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| To compare CAG repeats to other simple repeats and palindromic sequences , we examined the effect of DNA replication mutations , including alleles of pol alpha , pol delta , pol epsilon , and PCNA ( proliferating cell nuclear antigen ) , on tract stability . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| A comparison of the Vmax and Km for this derivative ( PMEApp ) and dATP demonstrated that the relative efficiency of the incorporation of this analog into the DNA chain is decreasing in the following order : pol delta approximately equal to pol epsilon approximately equal to pol epsilon * > pol alpha . ^^^ The DNA polymerases tested were inhibited by the reaction product ( PMEA terminated DNA chain ) with similar Ki / Km ratios ( pol alpha 0 . 2 ; pol delta , 0 . 1 ; pol epsilon 0 . 05 ; and pol epsilon * , 0 . 06 ) . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| We report here that hairpin and G ' 2 bimolecular tetraplex structures of the fragile 10 expanded sequence , d ( CGG ) ( n ) , effectively impede synthesis by three eukaryotic replicative DNA polymerases ( pol ) : pol alpha , pol delta , and pol epsilon . ^^^ The alleviatory effect of WRN is limited to pol delta ; neither pol alpha nor pol epsilon can traverse template d ( CGG ) ( n ) hairpin and tetraplex structures in the presence of WRN . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| In addition , the mutation rates of strains with the pol 2 4 mutation in combination with active site mutator mutations in Pol delta and Pol alpha suggest that Pol epsilon may proofread certain errors made by Pol alpha and Pol delta during replication in vivo . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| DNA polymerase alpha ( Pol alpha ) primase is required to prime synthesis of the leading strand and each Okazaki fragment on the lagging strand , whereas DNA polymerase delta ( Pol delta ) is required for the elongation stages of replication , a function it appears capable of performing on both leading and lagging strands , at least in the absence of DNA polymerase epsilon ( Pol epsilon ) . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Although the roles of replicative Pol alpha and Pol delta in chromosomal DNA replication are relatively well understood and well documented , the precise role of Pol epsilon in chromosomal DNA replication is not well understood . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Although the requirement of other replicative DNA polymerases , DNA polymerases alpha and delta ( Pol alpha and delta ) , for chromosomal DNA replication has been well documented by genetic and biochemical studies , the precise role , if any , of Pol epsilon in chromosomal DNA replication is still obscure . ^^^ Moreover , despite the decreased DNA synthesis , excess amounts of Pol alpha are loaded onto the chromatin template in Pol epsilon depleted extracts , indicative of the failure of proper assembly of DNA synthesis machinery at the fork . ^^^ These findings strongly suggest that Pol epsilon , along with Pol alpha and Pol delta , is necessary for coordinated chromosomal DNA replication in eukaryotic cells . . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| In contrast , the two adducts strongly blocked DNA synthesis by the purified human Polbeta and the purified catalytic subunits of yeast Polalpha , Poldelta , and Pol epsilon right before the lesion . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| Depending on which template primer was used , the HPMPAppKm value was 3 . 3 and 8 . 3 ( pol alpha ) , 3 and 0 . 82 ( pol delta ) or 2 fold ( pol epsilon ) higher than dATPKm . ^^^ The ability of HPMPA to accumulate in DNA decreased in the order pol epsilon > pol alpha > pol delta . ^^^ |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09884 and Q07864 |
Pubmed |
SVM Score :0.0 |
| NA |
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