| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.70276214 |
| Bcl XL interacts with Apaf 1 and inhibits Apaf 1 dependent caspase 9 activation . 0.70276214^^^ These experiments demonstrate that Bcl XL associates with caspase 9 and Apaf 1 , and show that Bcl XL inhibits the maturation of caspase 9 mediated by Apaf 1 , a process that is evolutionarily conserved from nematodes to humans . . 0.50122536^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Caspase 9 , Bcl XL , and Apaf 1 form a ternary complex . ^^^ An equivalent ternary complex was found to be present in mammalian cells involving Apaf 1 , the mammalian death protease caspase 9 , and Bcl XL , an anti apoptotic member of the Bcl 2 family . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| In cells overexpressing either Bcl 2 or Bcl xL , transient overexpression of Apaf 1 did not induce apoptosis . ^^^ This increase in paclitaxel or etoposide induced apoptosis of HL 60 / Apaf 1 cells was not associated with any significant alterations in Bcl 2 , Bcl xL , Bax , Fas , or Fas ligand expression . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| The expression of Diva inhibited the binding of Bcl XL to Apaf 1 , as determined by immunoprecipitation assays . ^^^ Thus , Diva represents a novel type of proapoptotic Bcl 2 homologue that promotes apoptosis independently of the BH 3 region through direct binding to Apaf 1 , thus preventing Bcl XL from binding to the caspase 9 regulator Apaf 1 . . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Like Ced 9 , the mammalian homologues Boo and Bcl xL interact with the human counterpart of Ced 4 , Apaf 1 , and thereby regulate apoptosis . . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Frameshift mutations in Fas , Apaf 1 , and Bcl 10 in gastro intestinal cancer of the microsatellite mutator phenotype . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Apaf 1 did not bind , however , to any of the six known mammalian prosurvival family members ( Bcl 2 , Bcl 10 ( L ) , Bcl w , A 1 , Mcl 1 , or Boo ) , or their viral homologs adenovirus E1B 19K and Epstein Barr virus BHRF 1 . ^^^ Endogenous Apaf 1 also failed to coimmunoprecipitate with endogenous Bcl 2 or Bcl 10 ( L ) , or with two proapoptotic relatives ( Bax and Bim ) . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Significantly , As ( 2 ) O ( 3 ) induced apoptosis of HL 60 / Bcr Abl and K 562 cells was associated with a decline in Bcr Abl protein levels , without any significant alterations in the levels of Bcl 10 ( L ) , Bax , Apaf 1 , Fas , and FasL . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Bcl xL does not inhibit the function of Apaf 1 . ^^^ Previous reports have suggested at least two distinct mechanisms : Bcl 2 and Bcl xL may inhibit either the formation of the cytochrome c / Apaf 1 / caspase 9 apoptosome complex ( by preventing cytochrome c release from mitochondria ) or the function of this apoptosome ( through a direct interaction of Bcl 2 or Bcl xL with Apaf 1 ) . ^^^ However , although Bcl xL did associate with Apaf 1 , it was unable to inhibit caspase activation induced by the addition of cytochrome c , even at much higher concentrations ( 1 5 microM ) . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Pro apoptotic apoptosis protease activating factor 1 ( Apaf 1 ) has a cytoplasmic localization distinct from Bcl 2 or Bcl 10 ( L ) . ^^^ Whereas Bcl 2 and Bcl 10 ( L ) were prominent on organelle membranes , endogenous Apaf 1 was cytosolic and did not colocalize with them , even when these pro survival proteins were overexpressed or after apoptosis was induced . ^^^ After the death stimuli , Bcl 2 and Bcl 10 ( L ) precluded the release of the Apaf 1 cofactor cytochrome c from mitochondria and the formation of larger Apaf 1 complexes , which are steps that presage apoptosis . ^^^ However , neither Bcl 2 nor Bcl 10 ( L ) could prevent the in vitro activation of Apaf 1 induced by the addition of exogenous cytochrome c . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| The possibility that Bcl 10 ( L ) inhibits cell death at a late ( postmitochondrial ) step in the death pathway is supported by this report of a novel apoptosis inhibitor , Aven , which binds to both Bcl 10 ( L ) and the caspase regulator , Apaf 1 . ^^^ Aven , a novel inhibitor of caspase activation , binds Bcl xL and Apaf 1 . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| CGP57148B treatment down regulated antiapoptotic XIAP , cIAP 1 , and Bcl 10 ( L ) , without affecting Bcl 2 , Bax , Apaf 1 , Fas ( CD 95 ) , Fas ligand , Abl , and Bcr Abl levels . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Whilst two groups previously detected an interaction between Bcl xL and Apaf 1 in immunoprecipitates , 1 , 2 another group found no interaction between Apaf 1 and any of ten individual members of the Bcl 2 family using the same experimental approach . 3 In this study , we aimed to resolve this discrepancy by monitoring the binding of Apaf 1 to three Bcl 2 family members within cells . ^^^ Using immunofluorescence and Western blot analysis , we show that whilst Apaf 1 is a predominantly cytoplasmic protein , Bcl 2 , Bcl xL and Bax mostly reside on nuclear / ER and mitochondrial membranes . ^^^ This pattern of localization is maintained when the proteins are co expressed in both normal and apoptotic cells , suggesting that Bcl 2 , Bcl xL or Bax do not significantly sequester cytoplasmic Apaf 1 to intracellular membranes . ^^^ In addition , we confirm that Apaf 1 does not interact with Bcl 2 and Bcl xL in immunoprecipitates . ^^^ Based on these data , we propose that Apaf 1 is not a direct , physiological target of Bcl 2 , Bcl xL or Bax . . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Differential requirement for Apaf 1 and Bcl 10 ( L ) in the regulation of programmed cell death during development . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Melanocytic cells expressed relatively high levels of Bcl 2 , Bcl 10 ( L ) , Mcl 1 , C IAP 1 , C IAP 2 , XIAP , Livin , and Apaf 1 . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Apaf 1 , Bcl xL , cytochrome c , and caspase 9 form the critical elements for cerebral vascular protection by erythropoietin . ^^^ Endothelial cell injury was evaluated by trypan blue , DNA fragmentation , membrane phosphatidylserine exposure , apoptotic protease activating factor 1 ( Apaf 1 ) , and Bcl XL expression , mitochondrial membrane potential , cytochrome c release , and cysteine protease activity . ^^^ Exogenous EPO maintains both genomic DNA integrity and cellular membrane asymmetry through parallel pathways that prevent the induction of Apaf 1 and preserve mitochondrial membrane potential in conjunction with enhanced Bcl XL expression . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| This review focuses on novel strategies to induce apoptosis in glioma cells by transduction with adenoviral vectors carrying a variety of apoptosis related genes , including Fas ligand , Fas , FADD , caspase 8 , p 53 , p33ING1 , p73alpha , Bax , Apaf 1 , caspase 9 , IkappaBdN , caspase 3 , Bcl 2 , and Bcl 10 ( L ) . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Mitochondrion harbors both antiapoptotic ( Bcl 2 , Bcl XL ) and apoptotic factors ( Smac / Diablo , Apaf 1 , cytochrome c ) . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| With a serial of Western blot analysis , it is revealed that gossypol induced cell cycle arrest is involved in P 21 up regulation and cyclin D 1 down regulation ; gossypol induced apoptosis triggers down regulation of anti apoptosis Bcl 2 members : Bcl 10 ( L ) , Bag 1 and Mcl 1 , up regulation of pro apoptosis Bcl 2 member Bak , activation of caspase 3 , 6 , 7 , 8 , and 9 , up regulation of Apaf 1 , release of cytochrome c ( cyto c ) from mitochondria , and activation of both DFF 45 and PARP . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Cytotoxicity , expression of genes encoding BCL 2 , BCL XL , SMAC , APAF 1 proteins and caspases 3 and 9 , the activity of caspases 3 and 9 and apoptosis induction were studied . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Forced expression of Apaf 1 restored the competence to complete the cytochrome c induced apoptotic program and this effect was prevented by overexpression of Bcl 10 ( L ) . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Changes in the expression of two anti apoptotic proteins , bcl 2 and bcl xL , and one pro apoptotic protein , apoptotic protease activating factor 1 ( Apaf 1 ) , were examined . ^^^ Genistein alone or cisplatin alone generally did not alter bcl 2 expression or bcl xL expression , but slightly increased Apaf 1 in some cell lines . ^^^ The combined treatment with genistein and cisplatin significantly reduced bcl 2 and bcl xL protein and increased Apaf 1 protein expression . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Identification of a Bcl XL binding region within the ATPase domain of Apaf 1 . ^^^ However , it has remained unclear whether a mammalian CED 9 homologue , Bcl XL , inhibits the function of the mammalian CED 4 homologue , Apaf 1 , by direct binding . ^^^ Since Bcl XL and the CED 4 like portion of Apaf 1 failed to exhibit a positive result in the assay , we prepared `` fragment libraries ' ' of bcl XL or apaf 1 cDNA . ^^^ By screening of the apaf 1 `` fragment library , ' ' we obtained nine clones interacting with Bcl XL , all containing the same region within the ATPase domain , designated BBR : the Bcl XL binding region . ^^^ These results indicate that Bcl XL directly binds to a specific region in Apaf 1 . . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| GDNF also protects against aluminum induced apoptosis but by upregulation of Bcl 10 ( L ) , thereby preventing the binding of cytochrome c to Apaf 1 . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| In particular , CD26 / DPPIV presence is associated with increased susceptibility to the mitochondrial pathway of apoptosis , documented by enhanced cleavage of poly ( ADP ribose ) polymerase ( PARP ) , caspase 3 and caspase 9 , Bcl xl , and Apaf 1 , as well as increased expression of death receptor 5 ( DR 5 ) . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Apoptosis related proteins were also expressed , including FLIP , bclx , Apaf 1 and XIAP , but expression levels differed among the three cell lines . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Rituximab ( anti CD 20 ) selectively modifies Bcl xL and apoptosis protease activating factor 1 ( Apaf 1 ) expression and sensitizes human non Hodgkin ' s lymphoma B cell lines to paclitaxel induced apoptosis . ^^^ Rituximab selectively down regulated Bcl xL and induced apoptosis protease activating factor 1 ( Apaf 1 ) expressions in Ramos cells . ^^^ Paclitaxel down regulated the expression of Bcl xL and inhibitor of apoptosis proteins ( c IAP 1 ) and up regulated the expression of Bad and Apaf 1 . ^^^ The findings identify two potential novel intracellular targets of rituximab mediated signaling in Ramos NHL cells ( i . e . , Bcl xL and Apaf 1 ) . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Evidence supporting the involvement of K+ efflux , the apoptosome ( caspase 9 , apoptosis activating factor 1 , APAF 1 , and Bcl xL ) , caspase 3 , c jun kinase , and p 53 in the p75NTR cell death pathway is discussed and regulatory roles for the p75NTR ectodomain and death domain are proposed . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Resveratrol selectively down regulated the expression of antiapoptotic proteins Bcl 10 ( L ) and myeloid cell differentiation factor 1 ( Mcl 1 ) and up regulated the expression of proapoptotic proteins Bax and apoptosis protease activating factor 1 ( Apaf 1 ) . ^^^ Paclitaxel down regulated the expression of Bcl 10 ( L ) , Mcl 1 , and cellular inhibitor of apoptosis protein 1 antiapoptotic proteins and up regulated Bid and Apaf 1 . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| This evidence that ER induced apoptosis mediated by the mitochondrial pathway was additionally supported by the finding that levels of Bcl 2 , Bcl xl , and XIAP protein were significantly lower ( P < 0 . 01 ) , and levels of Bax and Apaf 1 were elevated ( P < 0 . 02 ) in ER carcinomas versus those carcinomas from control or ER REP rats . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| In Apaf 1 ( / ) / Bcl2l ( / ) double mutant embryos , no cell death could be detected in the otic epithelium , demonstrating that the cell death regulated by the anti apoptotic Bcl2l isoform , Bcl 10 ( L ) in the otic epithelium is Apaf 1 dependent . ^^^ These results indicate important roles for both Apaf 1 and Bcl2l in inner ear development . . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Combined treatment with low concentrations of these two agents also acted synergistically to induce apoptosis in HepG 2 cells through induction of Bax and Apaf 1 , reduction of Bcl 2 and Bcl xL , and activation of caspase 3 , 8 , and 9 . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Downstream cellular pathways include FOXO3a , GSK 3beta , Bad , Bcl xL , NF kappaB , mitochondrial membrane permeability , APAF 1 and caspases . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Nevertheless , Apaf 1 knockout , overexpression of Bcl XL , and pharmacological inhibition of caspase activity did not prevent SAHA and butyrate induced cell death . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| The antiapoptotic proteins , Bcl 2 and Bcl xl , were downregulated by GSP , whereas the expression of the pro apoptotic protein , Bax , and the levels of cytochrome c release , Apaf 1 , caspase 9 , and cleaved caspase 3 ( p 19 and p 17 ) were markedly increased in JB 6 C141 cells . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Here we used embryonic fibroblasts derived from mice deficient in the multidomain proapoptotic members of the Bcl 2 family ( Bax and Bak ) and the apoptotic components of the apoptosome ( Apaf 1 and caspase 9 ) to unravel the cascade of events by which Bcl 10 ( S ) promotes apoptosis . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis revealed that the expression of Bcl xL , Bax , and Apaf 1 was not affected in cells transfected with sense or antisense AP 4 genes . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Examples of prominent CARD proteins are caspase 9 and Apaf 1 , which are involved in the intrinsic death pathway ; BCL 10 and CARD 11 , which mediate antigen receptor induced NF kappaB activation ; and receptor interacting protein ( RIP ) like interacting caspase like apoptosis regulatory protein kinase ( RICK ) and the nucleotide binding oligomerization domain ( NOD ) proteins , which induce NF kappaB activation in response to intracellular bacterial peptidoglycan . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| This indicated that 15d PGJ ( 2 ) induction of apoptosis was associated with a caspase 3 independent pathway . 15d PGJ ( 2 ) also induced down regulation of the 10 chromosome linked inhibitor of apoptosis ( XIAP ) , Bclx , and apoptotic protease activating factor 1 in SK Hep 1 cells but not in HepG 2 cells . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Here CED 9 and its mammalian homolog Bcl xL ( a member of the Bcl 2 family of cell death regulators ) were both found to interact with and inhibit the function of CED 4 . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Bcl XL cooperatively associates with the Bap 31 complex in the endoplasmic reticulum , dependent on procaspase 8 and Ced 4 adaptor . ^^^ Bcl XL , which binds directly but weakly to the polytopic transmembrane region of Bap 31 , indirectly and cooperatively associates with the Bap 31 cytosolic domain , dependent on the presence of procaspase 8 and Ced 4 . ^^^ Ced 4Deltac does not interact with Bcl XL but rather displaces it from Bap 31 , suggesting that an endogenous Ced 4 like adaptor is a normal constituent of the Bap 31 complex and is required for stable association of Bcl XL with Bap 31 in vivo . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Significantly , however , Bcl xL lacking BH 4 lost the ability both to bind CED 4 and antagonize its pro apoptotic activity . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| CED 4 directly interacts with the Bcl 2 homologue CED 9 ( or the mammalian Bcl 2 family member Bcl xL ) and the caspase CED 3 ( or the mammalian caspases ICE and FLICE ) . ^^^ Since E1B 19K and Bcl xL have functional similarity , we determined if E1B 19K interacts with CED 4 and regulates CED 4 dependent caspase activation . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| Alternatively spliced isoforms of certain apoptosis regulators , such as Bcl 10 , Ced 4 , and Ich 1 , have been shown to play opposing roles in regulating apoptosis . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| The ceBNIP 3 TM domain mediates interaction with both CED 9 and BCL XL . ceBNIP 3 interacts with CED 3 but co expression of CED 3 and ceBNIP 3 does not significantly enhance induction of cell death in the presence or absence of CED 4 . ceBNIP 3 kills mammalian cells by a caspase independent mechanism . ^^^ |
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| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| It has recently been demonstrated that CED 4 , an inducer of apoptosis for which no mammalian equivalent has been reported , can interact with CED 9 and Bcl 10 ( L ) . ^^^ Furthermore , we demonstrate that CED 4 not only interacts with Bcl 10 ( L ) but also with its homologue , Bcl 2 , and that the unstructured loop region present in Bcl 10 ( L ) and Bcl 2 can regulate the CED 4 interaction . ^^^ Lastly , we show that a BH 3 peptide that can inhibit Bcl 2 family interactions also inhibits the interaction between Bcl 10 ( L ) and CED 4 . . ^^^ |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14727 and Q07817 |
Pubmed |
SVM Score :0.0 |
| NA |
|