| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.83538135 |
| This BH 3 only molecule supports BH 3 as a death domain and favors a model in which BID represents a death ligand for the membrane bound receptor BAX . . 0.83538135^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.82347418 |
| Peptide mass fingerprinting identified this protein as Bid , a BH 3 domain containing protein known to interact with both Bcl 2 and Bax . 0.82347418^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.56355894 |
| Bid interaction with cardiolipin primarily orchestrates mitochondrial dysfunctions and subsequently activates Bax and Bak . 0.56355894^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| BH 3 is required for BID to interact with BCL 2 and BAX , as well as for its function as a death agonist . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| TNF alpha signals apoptosis through a bid dependent conformational change in Bax that is inhibited by E1B 19K . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Granzyme B mediated cytochrome c release is regulated by the Bcl 2 family members bid and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Death and survival signals determine active / inactive conformations of pro apoptotic BAX , BAD , and BID molecules . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid is a BH 3 domain only pro apoptotic member of the Bcl 2 family which interacts with Bax to regulate apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of Bid or induction of proapoptotic Bax was not observed during apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 family member Bfl 1 / A1 sequesters truncated bid to inhibit is collaboration with pro apoptotic Bak or Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| TRAIL induced translocation of Bax subsequent to the cleavage of Bid in parental cells . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Oligomerization of both Bax and Bak occurred independently of Bid cleavage and / or translocation . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The levels of Mcl 1 , Bax , Bid , Akt , c IAP 1 , c IAP 2 , XIAP and c FLIP were compared in all HL 60 lines . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| DDN induced an upregulation of proapoptotic Bax protein , and Bid cleavage . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The former include p 53 , Bid , Noxa , PUMA , Bax , TNF , TRAIL , Fas / FasL , PITSLRE , interferons , and c KIT / SCF . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 , Bax , Bak , Bid , and A 1 were used as indicators for activation of the mitochondrial pathway . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , fibroblast cells deficient in Bax and Bak , but not Bid , were resistant to bleomycin induced cell death . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| They also decreased the expression and / or activation of Fas , FasL , TNF , caspase 3 , 8 , and 9 , Bid , and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Jurkat T lymphocytes lack p 53 and Bax but contain p 73 and Bid and are killed by etoposide ( ETO ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax , Bad , Bid , Bim ) and anti apoptotic ( e . g . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| It shares a conserved domain , BH 3 , with other pro apoptotic proteins , Bax , Bak , Bid , and Hrk , and certain anti apoptosis proteins such as Bcl 2 and Bcl xL . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Although failing to interact with Bcl 2 , the Bax ( D68A ) and Bax ( E69A ) mutants retained the ability to bind to Bid , a putative Bax activating member of the Bcl 2 family , and collaborated with Bid in inducing apoptosis . ^^^ However , these results do not exclude the possibility that BH 3 dependent homodimerization of Bax or interactions with Bax activators such as Bid may either assist or be required for the cell death inducing mechanism of this protein . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Deletion of BH 4 rendered Bcl 2 ( and Bcl xL ) inactive but did not impair either Bcl 2 homodimerization or ability to bind to Bax or five other pro apoptotic relatives ( Bak , Bad , Bik , Bid or Bim ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Surface plasmon resonance was used to examine the kinetics of dimerization as a function of pH between the anti apoptotic protein Bcl XL ( applied in the mobile phase ) and three other members of the Bcl 2 family : Bcl 2 , Bax , and Bid ( immobilized on biosensor chips ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis . ^^^ Using isolated mitochondria and various BH 3 mutants of Bid , we demonstrate that direct binding of Bid to Bax is a prerequisite for Bax structural change and cytochrome c release . ^^^ Bcl xL can inhibit the effect of Bid by interacting directly with Bax . ^^^ Moreover , using mitochondria from Bax deficient tumor cell lines , we show that Bid induced release of cytochrome c is negligible when Bid is added alone , but dramatically increased when Bid and Bax are added together . ^^^ Taken together , our results suggest that , during certain types of apoptosis , Bid translocates to mitochondria and binds to Bax , leading to a change in conformation of Bax and to cytochrome c release from mitochondria . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Release of cytochrome c , Bax migration , Bid cleavage , and activation of caspases 2 , 3 , 6 , 7 , 8 , and 9 during endothelial cell apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Thus , the absence of cytochrome c release may be due to the lack of Bax translocation to mitochondria and / or Bid inactivation . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Proapoptotic members of the Bcl 2 family , including Bax , Bak , and Bid , directly trigger the mitochondrial release of apoptogenic cytochrome c and apoptosis inducing factor into the cytoplasm . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The pro apoptotic proteins , Bid and Bax , cause a limited permeabilization of the mitochondrial outer membrane that is enhanced by cytosol . ^^^ The pro apoptotic proteins , Bid and Bax , as well as factors present in Xenopus egg cytosol , each induced cytochrome c release when incubated with isolated mitochondria . ^^^ Bid and Bax caused complete release of cytochrome c but only a limited permeabilization of the outer membrane , as measured by the accessibility of inner membrane associated respiratory complexes 3 and 4 to exogenously added cytochrome c . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane . ^^^ Here we show that following binding to the BH 3 domain only proapoptotic protein Bid , Bax oligomerizes and then integrates in the outer mitochondrial membrane , where it triggers cytochrome c release . ^^^ Bax mitochondrial membrane insertion triggered by Bid may represent a key step in pathways leading to apoptosis . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Unlike Bax / Bak , the cytochrome c release induced by Bid / Bik was Ca ( 2+ ) independent , cyclosporin A insensitive , and respiration independent . ^^^ Furthermore , in contrast to Bax / Bak , Bid / Bik neither interacted with VDAC nor directly affected the VDAC activity in liposomes . ^^^ Consistently , Bid / Bik induced apoptosis without Deltapsi loss , whereas Bax induced apoptosis with Deltapsi loss . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Recently , a number of different second messengers ( calcium , ceramide derivatives , nitric oxide , reactive oxygen species ) and pro apoptotic proteins ( Bax , Bak , Bid , and caspases ) have been found to directly compromise the barrier function of mitochondrial membranes , when added to isolated mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Since cleavage of Bid serves to activate its cytotoxic activity , we wanted to determine if the p 18 form of Bax exhibited increased cytotoxicity compared to p 21 Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Here we report that recombinant t ( c ) Bid can act as a membrane destabilizing agent . t ( c ) Bid induces destabilization and breaking of planar lipid bilayers without appearance of ionic channels ; its destabilizing activity is comparable with that of Bax and at least 30 fold higher than that of full length Bid . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax , Bid and the permeabilization of the mitochondrial outer membrane in apoptosis . ^^^ Recent studies have provided insights into how Bax and Bid may operate synergistically to recruit mitochondria into the pathway and how GD 3 ganglioside , a metabolite of the sphingomyelin pathway , may also be used . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Expression of Bcl 10 ( L ) and Bid decreased significantly in the cadmium treated cells , although no apparent change in Bcl 2 and Bax expression was found . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Preservation of mitochondrial structure and function after Bid or Bax mediated cytochrome c release . ^^^ Proapoptotic members of the Bcl 2 protein family , including Bid and Bax , can activate apoptosis by directly interacting with mitochondria to cause cytochrome c translocation from the intermembrane space into the cytoplasm , thereby triggering Apaf 1 mediated caspase activation . ^^^ We now show that recombinant Bid and Bax cause complete cytochrome c loss from isolated mitochondria in vitro , but preserve the ultrastructure and protein import function of mitochondria , which depend on inner membrane polarization . ^^^ Thus , Bid and Bax act only on the outer membrane , and lesions in the inner membrane occurring during apoptosis are shown to be secondary caspase dependent events . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid induced cytochrome c release is mediated by a pathway independent of mitochondrial permeability transition pore and Bax . ^^^ We conclude that Bid possesses the biochemical activity to induce cytochrome c release through a mechanism independent of mitochondrial permeability transition pore and Bax . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Pro apoptotic members of the family , such as Bax , Bad and Bid , can induce the loss of outer membrane integrity with subsequent redistribution of pro apoptotic proteins such as cytochrome c that are normally located in the intermembrane spaces of mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Further study by co transfection experiments indicated that Bid but not Bax ( both were pro apoptotic proteins of Bcl 2 family ) blocked the inhibitory effect of Bcl 2 on Fas mediated apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The release of cytochrome c can be influenced by different Bcl 2 family member proteins , including , but not limited to , Bax , Bid , Bcl 2 , and Bcl 10 ( L ) . ^^^ Bax and Bid potentiate cytochrome c release , whereas Bcl 2 and Bcl 10 ( L ) antagonize this event . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Interestingly , in contrast to other Bax associating proteins such as Bcl 10 ( L ) and Bid , which require the BH 3 and BH 1 domains of Bax , respectively , for binding , the binding of MAP 1 to Bax appears to require all three BH domains ( BH 1 , BH 2 , and BH 3 ) of Bax , because point mutation of the critical amino acid in any one of these domains is sufficient to abolish its binding to MAP 1 . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Further , unlike purified mitochondria from wild type cells , those obtained from resistant cells did not release cytochrome c or apoptosis inducing factor in response to recombinant Bax or truncated Bid . ^^^ These results identify a defect in mitochondria ability to release intermembrane proteins in response to Bid or Bax as a mechanism of resistance to chemotherapeuetic drugs . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax and Bid ) can induce the release of SIMP ( e . g . cytochrome c ( cyt c ) and adenylate kinase 2 ( AK 2 ) ) in vivo and in vitro independent of PTP . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| BID dependent and BID independent pathways for BAX insertion into mitochondria . ^^^ In contrast to these findings , however , Bid null mouse embryo fibroblasts supported Bax insertion into mitochondria in response to death signaling by either TNFalpha or E1A , despite the fact that cytochrome c release from the organelle was inhibited . ^^^ We conclude , therefore , that a parallel Bid independent pathway exists in these cells for mitochondrial insertion of Bax and that , in the absence of Bid , cytochrome c release can be uncoupled from Bax membrane insertion . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Pro apoptotic cascade activates BID , which oligomerizes BAK or BAX into pores that result in the release of cytochrome c . ^^^ Thus , an activation cascade of pro apoptotic proteins from BID to BAK or BAX integrates the pathway from surface death receptors to the irreversible efflux of cytochrome c . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Full length Bid preferentially permeabilizes membranes and induces the formation of large conductance channels at neutral pH , when added to liposomes or bilayers containing both purified ANT and Bax , yet has no or little effect combined with ANT or Bax alone . t Bid acts on membranes containing ANT alone with the same efficiency as on those containing both ANT and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Progress has been made especially in the elucidation of the mechanisms of action of the Bcl 2 family members , in detail the formation of channels and their regulation in the mitochondrial membranes , conformational changes in Bax and Bak , and crosstalk of death receptor triggered apoptosis to the mitochondria by activation of Bax via Bid . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| No interaction of Bcl rambo with either anti apoptotic ( Bcl 2 , Bcl 10 ( L ) , Bcl w , A 1 , MCL 1 , E1B 19K , and BHRF 1 ) or pro apoptotic ( Bax , Bak , Bik , Bid , Bim , and Bad ) members of the Bcl 2 family was observed . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Through direct interaction with the voltage dependent anion channel ( VDAC ) , proapoptotic members of the Bcl 2 family such as Bax and Bak induce apoptogenic cytochrome c release in isolated mitochondria , whereas BH 3 only proteins such as Bid and Bik do not directly target the VDAC to induce cytochrome c release . ^^^ In isolated mitochondria , these antibodies prevented Bax induced cytochrome c release and loss of the mitochondrial membrane potential ( Deltapsi ) , but not Bid induced cytochrome c release . ^^^ When microinjected into cells , these anti VDAC antibodies , but not control antibodies , also prevented Bax induced cytochrome c release and apoptosis , whereas the antibodies did not prevent Bid induced apoptosis , indicating that the VDAC is essential for Bax induced , but not Bid induced , apoptogenic mitochondrial changes and apoptotic cell death . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Levels of Bax , Bid , and Bak proteins were similar in all lines , but levels of Bcl 2 were lower in MCF 10AT and MCF 10ATG3B cells than in MCF A cells , and Bcl 2 Bax heterodimerization progressively declined in the series . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Nevertheless , Bid was required , but Bax could be dispensed for the mitochondrial release of cytochrome c from mitochondria , suggesting that Bid could activate additional downstream molecules other than Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bak , a close homologue of Bax , colocalizes in these apoptotic clusters in contrast to other family members , Bid and Bad , which circumscribe the outer mitochondrial membrane throughout cell death progression . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The block in the apoptotic cascade was in the mitochondrial mechanism for cytochrome c release because purified mitochondria from Bak deficient cells failed to release cytochrome c or apoptosis inducing factor in response to recombinant Bax or truncated Bid . ^^^ The function of Bak is independent of Bid and Bax because recombinant Bak induced cytochrome c release from mitochondria purified from Bax ( / ) , Bid ( / ) , or Bid ( / ) Bax ( / ) mice . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| TNF alpha and truncated Bid induced Bax Bax cross linking , indicative of oligomerization , and E1B 19K expression during infection inhibited this TNF alpha mediated Bax oligomerization . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Here we report that a toxic regimen of METH did cause significant increases in the pro death Bcl 2 family genes BAD , BAX , and BID . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , a pro apoptotic Bcl 2 family protein , Bax , but not Bad or Bid , was translocated from cytosol to mitochondria in TCR stimulated thymocytes . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Purified mitochondria from Bid knockout mice , but not from Bax knockout mice , failed to release cytochrome c in response to autologous S 100 and GrB . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| These results led to the conclusion that Trp P 1 mainly drives the caspase 8 mediated pathway that involves Bid , accompanied by a delay in the p53 / NF kappa B mediated side pathway that involves Bax , Bcl 2 , and caspase 9 . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Granzyme B can cause mitochondrial depolarization and cell death in the absence of BID , BAX , and BAK . ^^^ Although GzmB directly cleaves the Bcl 2 family member BID on target cell entry , Bid deficient ( and Bax , Bak doubly deficient ) cells are susceptible to GzmB induced death , even though they fail to release cytochrome c from mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax deficiency has no effect on TRAIL induced caspase 8 activation and subsequent cleavage of Bid ; however , it results in an incomplete caspase 3 processing because of inhibition by XIAP . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of the tumor necrosis factor R1 / Fas receptor results in the cleavage of cytosolic BID to truncated tBID . tBID translocates to the mitochondria to induce the oligomerization of BAX or BAK , resulting in the release of cytochrome c ( Cyt c ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| No changes in the expression of Bax , Bid and Bik ( pro apoptotic proteins ) , or Bcl 2 ( an anti apoptotic protein ) were detected . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Dynamics of expression of apoptosis regulatory proteins Bid , Bcl 2 , Bcl 10 , Bax and Bak during development of murine nervous system . ^^^ We have used immunohistochemistry and immunoblotting to examine the expression of Bid and four other Bcl 2 family proteins ( Bcl 2 , Bcl 10 , Bax and Bak ) in the developing and adult murine central nervous system ( CNS ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| ML 1 cells incubated with either anisomycin or staurosporine exhibited Bax translocation , cytochrome c release , caspase 8 activation , and Bid cleavage ; only the latter two events were caspase dependent , confirming that they are consequences in this apoptotic pathway . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| However , other proteins analyzed [ namely , Apaf 1 , Bax , Bid , caspase 3 , caspase 8 , caspase 9 , 10 linked inhibitor of apoptosis protein ( XIAP ) , cellular inhibitor of apoptosis protein ( cIAP ) 1 , cIAP 2 , cytochrome c , Fas , Fas ligand , FLIP , p 53 , and poly ( ADP ribose ) polymerase ] were not affected by either rituximab or cisplatin . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid is the only known Bcl 2 family member that can function as an agonist of proapoptotic Bcl 2 related proteins such as Bax and Bak . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Unlike apoptosis mediated by death receptors , infection with proapoptotic E1B 19K mutant viruses did not induce cleavage of Bid but nonetheless induced changes in Bak and Bax conformation , Bak Bax interaction , caspase 9 and 3 activation , and apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| IFN gamma promoted TRAIL mediated activation of caspase 8 , Bcl 2 interacting domain death agonist ( Bid ) degradation , Bcl 2 associated 10 protein ( Bax ) translocation to mitochondria , cytochrome c release to the cytosol and activation of caspase 9 in these cell lines . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid induces cytochrome c impermeable Bax channels in liposomes . ^^^ The BH 3 domain only protein Bid induces a conformational change in Bax before its insertion into the outer membrane . ^^^ The mechanism by which Bid promotes Bax activation is not understood , and whether Bid is the only protein required for Bax activation is unclear . ^^^ In contrast , in the presence of Bid cut with caspase 8 ( cut Bid ) , Bax forms ionic channels in liposomes and planar bilayers . ^^^ This channel forming activity requires an interaction between cut Bid and Bax , and is inhibited by Bcl 10 ( L ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , 2 ME induced apoptosis in pancreatic cancer cells is mitochondria dependent as evident by the release of cytochrome c into the cytosol . 2 ME exposed cells exhibit Bid cleavage that is accompanied by the translocation of Bax into the mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| This substance promoted cell death via a mitochondrial signaling pathway , that is , by activation of Bid and Bax , loss of the mitochondrial transmembrane potential , release of cytochrome c , and activation of effector caspases , even under conditions of Bcl 2 overexpression . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Tyr / Phe deprivation decreases mitochondrial membrane potential , induces cleavage of Bid , increases translocation of Bax from the cytosol to mitochondria , and results in release of cytochrome c from the mitochondria to the cytosol . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Four molecules of the Bd 2 family ( BID , Bcl 2 , Bax , Bcl 10 ( L ) ) have been reported to be deaved during apoptosis , as has a cellular inhibitor of apoptosis ( XIAP ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We observed no detectable changes in the steady state levels of Bcl 10 ( L ) , Bax , and Bid , although TNF suppresses Bcl 2 expression . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Pro apoptotic members of the Bcl 2 family can be subdivided in two classes according to their structure : a group including Bax , Bak , and Bok that display Bcl 2 homology ( BH ) 1 , BH 2 and BH 3 domains and a second group including Bid ( BH 3 interacting domain death agonist ) , Bad , Bim ( Bcl 2 interacting mediator of cell death ) and several others that contain only a BH 3 domain , the BH 3 only proteins . ^^^ Although overexpression of Bid or Bim in cells leads to cytochrome c release , only Bid is able to trigger the release of cytochrome c through Bax activation when added directly to isolated mitochondria . ^^^ Our data suggest two functional classes of BH 3 only proteins : those such as Bid which directly activate Bax like proteins leading to mitochondrial membrane permeability and apoptosis and those such as Bim which inhibit anti apoptotic proteins and render the cells more susceptible to apoptogenic stimuli . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Survivin+ / mice appeared normal , but liver lysates revealed baseline low level activation of procaspase 8 , Bid , procaspase 9 , and procaspase 3 , with accumulation of Bax , and release of cytochrome c , indicating a proapoptotic state . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The active truncated form of BID ( tBID ) triggers the mitochondrial activation of caspase 9 by inducing the activation of BAK or BAX . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| At low concentrations at which caspase 8 cut Bid ( tBid ) alone has little effect on leakage , tBid augments the leakage caused by monomeric Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Adrenomedullin overexpressing T47D cells had higher levels of proteins involved in oncogenic signal transduction pathways ( such as Ras , Raf , PKC , and MAPKp 49 ) and lower levels of pro apoptotic proteins ( such as Bax , Bid , and caspase 8 ) than T47D cells transfected with empty vector . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax oligomerization in mitochondrial membranes requires tBid ( caspase 8 cleaved Bid ) and a mitochondrial protein . ^^^ Bax oligomerization can also be induced by incubating isolated mitochondria containing endogenous Bax with recombinant tBid ( caspase 8 cleaved Bid ) in vitro . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We observed down regulation of c Myc and of the anti apoptotic proteins Bcl 2 and Bfl 1 as well as high levels of the pro apoptotic proteins Bax and Bid . ^^^ Conversely , cells stably expressing NTD MR exhibited increased expression of Bcl 2 and Bfl 1 and diminished levels of Bid and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| TRAIL receptor and CD 95 signal to mitochondria via FADD , caspase 8 / 10 , Bid , and Bax but differentially regulate events downstream from truncated Bid . ^^^ Depletion of Bid from TRAIL or CD 95 activated cytosols blocked their capacity to mediate Cyt c release from mitochondria in vitro , whereas Bax depletion reduced it . ^^^ We conclude that FADD , caspase 8 / 10 , and caspase cleaved Bid are required for TRAIL receptor and CD 95 signaling to mitochondria , whereas Bax is a common accessory . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Moreover , Rho inhibition reduced expression of antiapoptotic Bcl 2 and Mcl 1 and increased proapoptotic Bid but had no effect on Bax or FLIP protein levels . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We find short peptides representing the alpha helical BH 3 domains of BID or BIM are capable of inducing oligomerization of BAK and BAX to release cytochrome c . ^^^ Another subset characterized by the BH 3 peptides from BAD and BIK can not directly activate BAX , BAK but instead binds antiapoptotic BCL 2 , resulting in the displacement of BID like BH 3 domains that initiate mitochondrial dysfunction . ^^^ These data support a two class model for BH 3 domains : BID like domains that `` activate ' ' BAX , BAK and BAD like domains that `` sensitize ' ' by occupying the pocket of antiapoptotic members . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| With TNF Bax translocation occurs as Bid is depleted and can be dissociated from the accumulation of tBid . ^^^ With staurosporine a role for full length Bid in Bax translocation can not be excluded but is not necessary as evidenced by the data with TNF . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid , Bax , and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane . ^^^ Bid , or its BH 3 domain peptide , activated monomeric Bax to produce membrane openings that allowed the passage of very large ( 2 megadalton ) dextran molecules , explaining the translocation of large mitochondrial proteins during apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The levels of anti apoptotic proteins Bcl 2 and Bcl 10 ( L ) were increased , and the cellular levels of pro apoptotic proteins Bid and Bax were reduced . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Colocalization of BAX with BID and VDAC 1 in nimesulide induced apoptosis of human colon adenocarcinoma COLO 205 cells . ^^^ In the present study the effect of nimesulide ( NIM ) , a specific COX 2 inhibitor , on apoptosis and interactions between BCL 2 family death promoters BAX and BID and BAX and VDAC 1 were examined in human colon adenocarcinoma COLO 205 cells . ^^^ This was accompanied by : ( 1 ) a decrease in intracellular prostaglandin ( PG ) E content ; ( 2 ) subcellular redistribution and aggregation of BAX and BID on organellar membranes and within the nucleus ; ( 3 ) colocalization of BAX with BID and BAX with VDAC 1 on organelles ; and ( 4 ) survival of cells with the highest BCL 2 aggregation . ^^^ A similar pattern of subcellular redistribution and colocalization of BAX with BID and BAX with VDAC 1 suggests that BAX ( in association with BID ) controls the function of VDAC 1 and its permeability for apoptogenic factors released from mitochondria of COLO 205 cells stimulated to apoptosis with NIM . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The release of cytochrome c is the central gate in turning on / off apoptosis , and is regulated by the interaction of proapoptotic proteins , including Bid , Bax and Bak , and antiapoptotic proteins including Bcl 2 and Bcl 10 ( L ) , and a specific class of inhibitors of apoptosis proteins ( IAPs ) including Akt , survivin , and heat shock proteins . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 members can either be anti ( Bcl 2 , Bcl 10 ( L ) , Bcl w ) or pro apoptotic ( Bax , Bak , Bid , Bad , Bcl 10 ( S ) ) . ^^^ The expression of the pro apoptotic members Bax , Bak , Bid , and Bcl 10 ( S ) was significantly ( P < 0 . 05 ) decreased after TPN . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The release of cytochrome c can be influenced by different Bcl 2 family member proteins , including Bax , Bid , Bcl 2 , and Bcl 10 ( L ) . ^^^ Bax and Bid potentiate cytochrome c release , whereas Bcl 2 and Bcl 10 ( L ) antagonize this event . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Whereas IL 4 and PPARgamma ligands failed to induce cleavage of Bid and release of cytochrome c from mitochondria , they caused translocation of the proapoptotic protein Bax from cytoplasm to mitochondria with a concomitant decrease in the Bcl 10 ( L ) level . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , TRO increased the levels of proapoptotic proteins , Bad , Bax , release of cytochrome c , and cleavage of Bid in a time dependent manner . ^^^ Furthermore , SP 600125 pretreatment effectively prevented the TRO mediated changes in Bad , Bax , Bid cleavage , and cytochrome c release . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In human KB epithelial cells expressing the caspase resistant mutant crBAP 31 , Fas stimulation resulted in cleavage of BID and insertion of BAX into mitochondrial membrane , but subsequent oligomerization of BAX and BAK , egress of cytochrome c to the cytosol , and apoptosis were impaired . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Recent work shows that two pro apoptotic Bcl 2 family proteins , Bax and Bid , as well as the mitochondrion specific lipid cardiolipin may cooperate in chemically defined liposomes to generate a protein permeable conduit , relaunching the debate on the identity of the pore responsible for mitochondrial membrane permeabilization during apoptosis . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Probably due to the weak starting signal , caspase 3 further activated caspase 8 which , through the Bid cleavage and Bax translocation into the mitochondria , provided an autocatalytic support for the apoptotic program . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The expression levels of known effector molecules mediating mitochondrial signaling , such as Bax and Bid , and the antagonistic molecule Bcl 2 did not influence sensitivity of B CLL cells to TC A . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Bid induced apoptosis by Bcl 2 . tBid insertion , Bax translocation , and Bax / Bak oligomerization suppressed . ^^^ Bid , Bax , and Bak ) or anti apoptotic ( e . g . ^^^ Bcl 2 also prevented Bid induced Bax translocation from cytosol to the membrane bound organellar fraction . ^^^ Finally , Bcl 2 diminished Bid induced oligomerization of Bax and Bak within the membrane bound organellar fraction , shown by cross linking experiments . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Although the BH 3 domain of Bid can promote the pro apoptotic assembly and function of Bax / Bak by itself , other BH 3 domains do not function as such . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In addition , enteric neuron number is normal in Bax ( / ) and Bid ( / ) mice , suggesting that , in contrast to most of the rest of the nervous system , programmed cell death is not important for determining enteric neuron numbers . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Treating HCT 116 cells with THG results in caspase 8 activation ; Bid cleavage ; Bax conformational change and mitochondrial translocation ; the release of cytochrome c , Smac / Diablo , and Omi / HtrA2 into the cytosol ; caspase 3 activation ; and apoptosis . ^^^ The caspase 8 specific inhibitor z IETD fmk , as well as pan caspase inhibitor z VAD fmk , but not the calpain inhibitor E 64d , prevents Bid cleavage , Bax conformational change , and subsequent caspase 3 processing and apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of BID , a `` BH 3 domain only ' ' Bcl 2 family member , triggers the oligomerization of proapoptotic family members Bak or Bax , resulting in the release of mitochondrial proteins to cytosol . ^^^ TRAIL induced cytochrome c release and apoptosis in wild type , Bid ( / ) , Bax ( / ) , or Bak ( / ) MEFs , but not in Bax ( / ) Bak ( / ) double knockout ( DKO ) MEFs . ^^^ Unlike cytochrome c release , TRAIL induced Smac / DIABLO release was blocked in Bid ( / ) , Bax ( / ) , Bak ( / ) , or DKO MEFs , suggesting the differential regulation of these mitochondrial proteins during apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Previous results demonstrated that a synthetic BH 3 peptide mimics the ability of the BH 3 only protein Bid to promote Bax insertion and cytochrome c ( cyt c ) release from neural cell mitochondria . ^^^ Recombinant Bax ( 100 nm ) alone did not release cyt c from adult rat brain mitochondria ; however , when BH 3 peptide or caspase 8 cleaved Bid ( cBid ) was added , robust cyt c release was achieved that was inhibited completely by 200 microm dibucaine or propranolol . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Caspase 8 cleaves BID to tBID , which targets mitochondria and induces oligomerization of BAX and BAK within the outer membrane , resulting in release of cytochrome c from the organelle . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In addition BH 3 only proteins of this family such as Bid are suggested to act as critical death inducing ligands via interactions with pro and anti apoptotic Bcl 2 family proteins with Bax or Bak at the mitochondrial surface . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Proteasome inhibitors did not alter the levels of expression of the proapoptotic Bcl 2 family proteins , Bax and Bid , prior to the onset of apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Our results further demonstrate that TG induced apoptosis is coupled with DR 5 upregulation and caspases 8 and 3 activation , as well as Bid cleavage in both Bax proficient and deficient cells , although caspase 3 activation was reduced in Bax deficient cells . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We showed increased activation of both Bid and Bax in A 549 cells subjected to hyperoxia . ^^^ Bax activation involved a Bid assisted conformational change . ^^^ We discovered that the response to hyperoxia in vivo predominantly involved the activation of the Bid / caspase 8 pathway without apparent increases in Bax expression . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The single knockout of Bax or Bak ( but not Bid ) or the transfection enforced expression of mitochondrion targeted ( but not endoplasmic reticulum targeted ) Bcl 2 conferred protection against CPX ( but not NFX * ) induced MMP and death . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| DNC 8 did not inhibit the cleavage of Bid or the activation of Bax . ^^^ Exogenous addition of sphingosine also induced activation of Bax via a caspase 8 and Bid independent mechanism . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| It was revealed that LIGHT treatment resulted in down regulation of anti apoptosis Bcl 2 family member : Bcl 2 , Bcl 10 ( L ) , Bag 1 , and Mcl 1 ; up regulation of pro apoptosis Bcl 2 family member : Bak and Ser ( 112 ) phosphor Bad ; down regulation of pro apoptosis Bcl 2 member Bax ; the other pro apoptosis member Bid remains unaltered . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bisphenol A diglycidyl ether induced apoptosis involves Bax / Bid dependent mitochondrial release of apoptosis inducing factor ( AIF ) , cytochrome c and Smac / DIABLO . ( 1 ) Bisphenol A diglycidyl ether ( BADGE ) is a peroxisome proliferator activated receptor gamma ( PPAR gamma ) antagonist , which is able to induce apoptosis in tumor cells independently of PPAR gamma in caspase dependent and independent manners . ^^^ Additionally , BADGE promotes TRAIL induced apoptosis . ( 2 ) We report that BADGE activates via Bax and caspases 2 and 8 both the intrinsic and extrinsic apoptotic pathways using Bid as a shunt . ( 3 ) BADGE stimulates the mitochondrial release of apoptosis inducing factor ( AIF ) , cytochrome c and second mitochondria derived activator of caspase / direct IAP binding protein with low pl ( Smac / DIABLO ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Nonredundant role of Bax and Bak in Bid mediated apoptosis . ^^^ We show that GrB cleaved Bid and C 8 cleaved Bid differ in size and utilize preferentially Bax and Bak , respectively , to promote cytochrome c release from mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Experiments with the use of double and triple immunolabelling visualized the colocalization of proapoptotic proteins such as Bax with Bid , Bax with Bid and voltage dependent anion channel protein ( VDAC 1 ) , and Bax with Bid and caspase 8 , on organellar membranes and within the nucleus . ^^^ Application of this technique in combination with EF EM technique augments our knowledge on the precise identification and relationship of subcellular structures containing Bax , Bid , VDAC 1 and caspase 8 . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In dissecting the mechanism underlying this synergistic effect , we found that treatment with etoposide alone resulted in the upregulation of Bax , while the level of truncated Bid ( tBid ) was unchanged . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In the present study we used laser scanning cytometry , confocal microscopy and immunogold electron microscopy to analyze the expression , aggregation and co localization of caspase 8 , Bid , Bax and VDAC 1 . ^^^ Apoptosis in HC 11 mouse MEC manifested with a simultaneous increase in expression and subcellular aggregation of caspase 8 , Bid , Bax and VDAC 1 . ^^^ Experiments with double and triple staining immunoelectron microscopy showed a co localization of Bax / Bid , caspase 8 / Bax / Bid , and Bax / VDAC 1 , on the membranes of mitochondria , Golgi apparatus , rough endoplasmic reticulum , nuclear envelope , nuclear pore , and within the nucleus . ^^^ In conclusion , the observed pattern of changes in aggregation and subcellular localization of caspase 8 , Bid , Bax and VDAC 1 during TGF beta 1 induced apoptosis in HC 11 mouse MEC suggests an interaction between these proteins and formation of multimeric complexes on organellar membranes , thus controlling their permeability for intracellular mediators of apoptosis . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In contrast , higher expression levels of 12 apoptosis related genes ( caspase 3 , 6 , 7 , 8 , 9 , 10 , 12 , BAD , BAX , BID , Fas , and FasL ) were shown in chorionic villi from RPL patients than those from normal controls . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Investigation of the role of the C terminus of Bax and of tc Bid on Bax interaction with yeast mitochondria . ^^^ In mammals , Bax interaction with the caspase 8 generated truncated form of Bid ( tc Bid ) is believed to promote a conformational change necessary for the insertion of Bax into mitochondria . ^^^ We coexpressed Bax and tc Bid in yeast and found that native Bax functions are not stimulated by tc Bid , whereas functions of an active variant with a modified CT are . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Involvement of mitochondria in acetaminophen induced apoptosis and hepatic injury : roles of cytochrome c , Bax , Bid , and caspases . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with tetrandrine caused the upregulation of p 53 , downregulation of Bcl 10 ( L ) , cleavage of Bid and Bax , and release of cytochrome c , which were accompanied by activation of caspases 9 , 3 and 8 . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| This review focuses on the interactions of two of the major pro apoptotic Bcl 2 family members , Bax and Bid , with mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| SBHA appeared to sensitize melanoma to TRAIL induced apoptosis by up regulation of pro apoptotic proteins in the TRAIL induced apoptotic pathway such as caspase 8 , caspase 3 , Bid , Bak , and Bax , and up regulation of the BH 3 domain only protein , Bim . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| This family of proteins now includes both anti apoptotic molecules such as Bcl 2 and Bcl 10 ( L ) , and pro apoptotic molecules such as Bax , Bak , Bid , and Bad . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The cytoprotection afforded by hepatocyte growth factor was mediated in part by the stimulation of FLICE like inhibiting protein expression , the attenuation of death inducing signal complex formation , and the inhibition of Bid and Bax activation . ^^^ The inhibition of Bid / Bax induced cell death by hepatocyte growth factor primarily involved p 38 MAPK and in part Akt dependent pathways but not ERK1 / ERK2 . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In this study , expression of Mcl 1 , Bid , Bcl 2 , and Bax were investigated in septic neutrophils . ^^^ Mcl 1 , Bid , Bcl 2 , and Bax mRNA expression was detected by reverse transcriptase polymerase chain reaction and protein determined by Western blot analysis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| DDN induced cytochrome c release , Bax translocation , cleavage of Bid and Bad , and activation of caspase 3 , 8 , 9 upon the induction of apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| MIF , like G CSF , delayed cleavage of the proapoptotic members of the Bcl 2 family Bid and Bax in neutrophils , suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| MG 132 effectively cooperated with Apo2L / TRAIL to induce apoptosis in both Bax proficient and deficient cells that was coupled with caspases 8 and 3 activation and Bid cleavage . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Expression of other proapoptotic genes , including BAX , BAD , BID , and PUMA , was unaffected by treatment with 5 aza deoxycytidine . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| These results suggest that Akt inhibits BID mediated apoptosis downstream of BID cleavage via promotion of mitochondrial hexokinase association and antagonism of tBID mediated BAX and BAK activation at the mitochondria . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Analysis of mitochondrial events in U 937 cells exposed to TRAIL / HDAC inhibitors revealed enhanced Bid activation and Bax translocation , loss of mitochondrial membrane potential , and cytoplasmic release of cytochrome c , Smac / DIABLO , and apoptosis inducing factor . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Semi quantitative RT PCR was used to measure Bid , Bax and Bcl 2 mRNA expressions . ^^^ RESULTS : Expressions of Bid and Bax in gastric adenocarcinoma tissues without H pylori infection , with cagA H pylori infection and cagA+ H pylori infection increased significantly in turn ( Bid , 0 . 304 , 0 . 422 and 0 . 855 respectively , P < 0 . 05 ; Bax , 0 . 309 , 0 . 650 and 0 . 979 respectively , P < 0 . 05 ) . ^^^ Expressions of Bid , Bax and Bcl 2 in resection margin tissues without H pylori infection , with cagA H pylori infection and cagA+ H pylori infection increased significantly in turn ( Bid , 0 . 377 , 0 . 686 and 0 . 939 respectively , P < 0 . 05 ; Bax , 0 . 353 , 0 . 645 and 1 . 001 respectively , P < 0 . 05 ; Bcl 2 , 0 . 371 , 0 . 487 and 0 . 619 respectively , P < 0 . 05 ) . ^^^ In H pylori negative specimens , expressions of Bid and Bax correlated negatively with that of Bcl 2 respectively in adenocarcinoma tissues ( Bid vs Bcl 2 , r= 0 . 409 , P < 0 . 05 ; Bax vs Bcl 2 , r= 0 . 451 , P < 0 . 05 ) . ^^^ In H pylori positive specimens , expressions of Bid and Bax did not correlate with that of Bcl 2 in adenocarcinoma tissues ( Bid vs Bcl 2 , r=0 . 187 , P > 0 . 05 ; Bax vs Bcl 2 , r=0 . 201 , P > 0 . 05 ) , but correlated positively with that of Bcl 2 respectively in resection margin tissues ( Bid vs Bcl 2 , r=0 . 331 , P < 0 . 05 ; Bax vs Bcl 2 , r=0 . 295 , P < 0 . 05 ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation followed by western blot analysis shows that low extracellular pH enhances the association of truncated Bid with Bax during treatment with TRAIL . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid , but not Bax , regulates VDAC channels . ^^^ In contrast to Bax , another pro apoptotic protein ( Bid ) proteolytically cleaved with caspase 8 affected the voltage gating of VDAC by inducing channel closure . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Moreover , we have shown that unlike the pro apoptotic protein Bid , the toxicity associated with the transient expression of Bcl 2 occurs independent of the activity of the endogenous Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Caspase 8 and Bid were cleaved in Colo 357 cells exposed to gemcitabine , and there was no correlation with either Bcl xL or with Bax expression . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Treatment of HepG 2 cells with IH 901 also induced the cleavage of cytosolic factors such as Bid and Bax and translocation of truncated Bid ( tBid ) to mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The transcription independent activation of Bax by p 53 occurred with similar kinetics and concentrations to those produced by activated Bid . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We extend the findings with Bad to demonstrate that Us 3 blocks apoptosis induced by overexpression of Bid , a factor parallel to Bad in the apoptotic pathway , and Bax , a factor downstream of Bad in the apoptotic pathway . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| To date , structural studies have been performed on six Bcl 2 family members encompassing both anti ( Bcl 10 ( L ) , Bcl 2 , KSHV Bcl 2 , Bcl w ) and pro apoptotic ( Bax , Bid ) members . ^^^ The hydrophobic groove of the pro apoptotic protein , Bid protein , is neither as long nor as deep as that found in Bcl 10 ( L ) , Bcl 2 , or Bax . ^^^ In addition , Bid contains an extra alpha helix , which is located between alpha 1 and alpha 2 with respect to Bcl 10 ( L ) , Bcl 2 , and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Proapoptotic members of the Bcl 2 family , especially Bax and Bid , have been extensively analyzed for the ability to form channels in membranes and to regulate preexisting channels . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Minocycline induced Bcl 2 , which accumulated in mitochondria and interacted with death promoting molecules including Bax , Bak , and Bid . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The glioma cells showed no consistent down regulation of the pro apoptotic proteins Fas , Fadd , caspase 3 , caspase 8 , caspase 9 , Apaf 1 , Bid , Bad , or Bax , and no consistent up regulation of the anti apoptotic proteins Bcl 10 or Bcl 2 . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In aged coronary arteries there was an increased expression of TNFalpha , TNFbeta , and caspase 9 ( microarray , real time PCR ) , as well as increased caspase 9 and caspase 3 activity , whereas expression of TNFR 1 , TNFalpha converting enzyme ( TACE ) , Bcl 2 , Bcl 10 ( L ) , Bid , Bax , caspase 8 , and caspase 3 were unchanged . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Hypoxia mediated down regulation of Bid and Bax in tumors occurs via hypoxia inducible factor 1 dependent and independent mechanisms and contributes to drug resistance . ^^^ The physiological relevance of Bid and Bax down regulation was confirmed in tumors in vivo . ^^^ The contribution of Bid and / or Bax down regulation to drug responsiveness was demonstrated by the relative resistance of normoxic cells that had no or reduced expression of Bid and / or Bax and by the finding that forced expression of Bid in hypoxic cells resulted in increased sensitivity to the topoisomerase 2 inhibitor etoposide . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Yeast has allowed new information to be gained about crucial aspects of the interaction between proapoptotic proteins Bax and Bid and mitochondria , namely , their addressing and insertion into the mitochondrial outer membrane and their ability to induce the relocalization of cytochrome c . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The present study , which aims to investigate whether ( 1 ) cerulein induces apoptotic gene expression ( bax , bid , p 53 ) in pancreatic acinar AR42J cells and ( 2 ) cerulein induced gene expression is mediated by intracellular Ca ( 2+ ) , monitored the gene expression profile in the cells treated with the Ca ( 2+ ) chelator BAPTA AM . ^^^ Cerulein induced bid , bax , and p 53 mRNA expression , which was inhibited in the cells treated with cerulein and cultured in the presence of BAPTA AM . ^^^ The present results suggest that increase in the free cytosolic Ca ( 2+ ) may be the upstream event of apoptotic gene ( bax , bid , p 53 ) expression , which contribute to cerulein induced apoptosis in pancreatic acinar cells . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| PTPC may also interact with tumor suppressor proteins ( i . e . , Bax and Bid ) , oncoprotein homologues of Bcl 2 and some viral proteins , which can regulate apoptosis induced by pore opening . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax , Bad and Bid were largely present within the cytoplasm before and after seizures , although some Bax and , following seizures , truncated Bid was detected in mitochondria . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We demonstrate constitutive expression of BAX in virtually all embryos at all stages of development , and variable expression of BCL 2 , BCL XL , BCL W , MCL 1 BAK , BAD , BOKL , BID , BIK , and BCL XS . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Diffuse large B cell lymphomas with germinal center B cell like differentiation immunophenotypic profile are associated with high apoptotic index , high expression of the proapoptotic proteins bax , bak and bid and low expression of the antiapoptotic protein bcl xl . ^^^ Therefore , the bcl6 / CD10 / MUM1 / CD138 differentiation immunophenotypic profiles were studied in relation to ( a ) the apoptotic index , ( b ) the apoptosis associated bcl 2 family proteins bcl 2 , bcl xl , bax , bak , bad and bid , ( c ) the proliferation index ( Ki 67 ) and ( d ) the cell cycle proteins cyclin A , cyclin B 1 , cyclin D 3 , cyclin E , p 53 , Rb , p 16 and p 27 in 79 cases of diffuse large B cell lymphomas . ^^^ The expression of bax , bak and bid and the apoptotic index were significantly higher in the germinal center B cell like profile than in the nongerminal center B cell like profile ( P=0 . 045 , 0 . 018 , 0 . 003 and 0 . 034 , respectively ) . ^^^ The expression of bcl 6 and CD 10 showed significant positive correlation with the expression of bax ( r=0 . 659 , P < 0 . 001 and r=0 . 240 , P=0 . 033 , respectively ) , bak ( r=0 . 391 , P < 0 . 001 and r=0 . 233 , P=0 . 039 , respectively ) and bid ( r=0 . 652 , P < 0 . 001 and r=0 . 238 , P=0 . 035 , respectively ) and significant negative correlation with the expression of bcl xl ( r= 0 . 536 , P < 0 . 001 and r= 0 . 250 , P=0 . 029 , respectively ) . ^^^ The expression of MUM 1 showed significant negative correlation with the expression of bax ( r= 0 . 276 , P=0 . 014 ) and bid ( r= 0 . 266 , P=0 . 018 ) and significant positive correlation with the expression of bcl xl ( r=0 . 238 , P=0 . 037 ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We further show that a JNK specific inhibitor SP 600125 completely blocks TNF induced JNK activation , Bid cleavage , and Bax mitochondrial translocation , but only partially inhibits cytochrome c release and EC death , suggesting that TNF induces both JNK dependent and JNK independent apoptotic pathways in EC . ^^^ Mitochondria specific expression of a constitutively active ASK 1 strongly induces EC apoptosis without JNK activation , Bid cleavage , and Bax mitochondrial translocation . ^^^ Moreover , specific knockdown of Trx 2 in EC increases TNF / ASK1 induced cytochrome c release and cell death without increase in JNK activation , Bid cleavage , and Bax translocation . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Infection induced apoptosis of EC was associated with decreased level of Bid and accumulation of Bad , while cytosolic level of Bax remained relatively unchanged . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Molecular dynamics study of peptide segments of the BH 3 domain of the proapoptotic proteins Bak , Bax , Bid and Hrk bound to the Bcl xL and Bcl 2 proteins . ^^^ In the current work , these proteins complexed with 10 ( 16BH3 ) , where 10 designates the proapoptotic proteins Bak , Bax , Bid and Hrk , have been modeled in order to establish a pharmacophoric hypothesis that must be present in any ligand capable of binding with the antiapoptotic proteins Bcl 2 and Bcl xL . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Caspase 8 cleaves full length Bid , resulting in truncated p 15 tBid . p 15 tBid is potently apoptotic and activates the multidomain Bcl 2 protein , Bax , resulting in release of cytochrome c from mitochondria . ^^^ Bid translocates to mitochondria with identical kinetics as Bax . ^^^ Furthermore , it does not require Bid to interact directly with other Bcl 2 family proteins , such as Bax . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid truncation , bid / bax targeting to the mitochondria , and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony stimulating factor . ^^^ Using digitonin based subcellular fractionation and Western blotting , we found that spontaneous apoptosis of human neutrophils ( after approximately 20 h of culture ) was associated with translocation of two proapoptotic Bcl 2 homologues , Bid and Bax , to the mitochondria and truncation of Bid , with subsequent release of Omi / HtrA2 and Smac / DIABLO into the cytosol . ^^^ Whereas inhibition of Bax targeting to the mitochondria and inhibition of caspase activation by G CSF were dependent on protein synthesis , Bid truncation and redistribution were prevented by G CSF regardless of the presence of the protein synthesis inhibitor cycloheximide . ^^^ Together our results support an integrative role of the mitochondria in induction and / or amplification of caspase activity and show that G CSF may act by blocking Bid / Bax redistribution and inhibiting caspase activation . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Expression of modulators of apoptosis was not changed in the resistant cells , including TRAIL receptors , cFLIP , Bax , Bid , or IAP proteins . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Instrumental to this clarification is the emerging evidence that CL ( cardiolipin ) , an unusual membrane lipid that is predominantly present in mitochondria , is required for the action of major pro apoptotic proteins like Bid and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| A contemporary view of this process is that in so called type 2 cells death receptor activated caspase 8 cleaves the Bcl 2 family member Bid , which generates a truncated Bid fragment that collaborates with Bax , another Bcl 2 relative , to promote the release of mitochondrial factors necessary for activation of executioner caspases and apoptosis . ^^^ Analysis of the TRAIL proteolytic cascade following gene silencing of specific pathway components revealed that caspase 2 is necessary for efficient cleavage of Bid ; however , caspase 2 proteolytic processing , which occurs downstream of Bax , is not necessary for its role in Bid cleavage . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| M11L binds constitutively to human Bak and , under some inducible conditions , to human Bax as well , but not to the other Bcl 2 family members ( Bad , Bid , Bcl 2 ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Following caspase 8 activation , Bid , a proapoptotic Bcl 2 member , was cleaved and this cleavage then triggered Bax conformational changes and Bax translocation from cytosol to mitochondrial membrane . ^^^ Moreover , selective inhibition of caspase 8 activity by a synthetic caspase inhibitor ( IETD FMK ) or overexpression of a viral protein ( CrmA ) suppressed the cleavage of Bid , conformational changes of Bax , cytochrome c release , and apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with NOCF caused dose dependent upregulation of cytosolic factors , such as Bax and Bid , and release of cytochrome c , which were accompanied by activation of caspase 9 . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , pneumococcus infected human alveolar epithelial cells showed Bid cleavage and reduced levels of Bcl 2 and Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Translocation of Bax and Bid to mitochondria , endoplasmic reticulum and nuclear envelope : possible control points in apoptosis . ^^^ Immunoelectron studies have demonstrated that Bax and Bid translocate from cytoplasm to mitochondria where they initiate mitochondrial dysfunction and cytochrome c release . ^^^ Bax and Bid were also localized in ER and nuclear envelope . ^^^ Since ER and mitochondria function as intracellular Ca2+ storage , we hypothesize that Bax and Bid are involved in the emptying of ER Ca2+ pool , triggers secondary changes in mitochondrial Ca2+ levels that contribute to cytochrome c release and cell death . . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Membrane insertion fragments of Bcl xL , Bax , and Bid . ^^^ Here , we report on the membrane insertion properties of different segments from antiapoptotic Bcl 10 ( L ) and proapoptotic Bax and Bid , that correspond to defined alpha helices in the structure of their soluble forms . ^^^ Surprisingly , the amphipathic helices alpha 6 of Bcl 10 ( L ) and Bax and alpha 7 of Bid do insert in membranes only as part of the alpha 5 alpha6 ( Bcl 10 ( L ) and Bax ) or alpha 6 alpha7 ( Bid ) hairpins but not when assayed individually . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Central mediators of delayed neuronal cell death are two families of molecules : a group of cysteine aspartate proteases , called caspases , and molecules of the bcl 2 family , e . g . bcl 2 , bax , and bid . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Coexposure of cells to marginally toxic concentrations of TRAIL and FP ( 24 h ) synergistically increased mitochondrial injury ( eg , cytochrome c , AIF , Smac / DIABLO release ) , cytoplasmic depletion of Bax , activation of Bid as well as caspase 8 and 3 , PARP cleavage , and apoptosis . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Further , upon cDDP treatment , the levels of Fas , Bax and Bid remained unchanged , whereas Bcl 2 and p Bad were reduced at late times ( 120 hr ) after treatment . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Cadmium induces apoptotic cell death in WI 38 cells via caspase dependent Bid cleavage and calpain mediated mitochondrial Bax cleavage by Bcl 2 independent pathway . ^^^ Taken together , our results suggest that Cd induced apoptosis was thought to be mediated at least two pathways ; caspase dependent Bid cleavage , and the other is calpain mediated mitochondrial Bax cleavage . ^^^ Moreover , we found that the function of Bid and Bax was not dependent of Bcl 2 , and that ROS can also contribute in the Cd induced cell death . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Cytochrome C release was due neither to a direct effect of caspases onto mitochondria nor to an interaction of Bax or Bid with the mitochondrial membrane but could be related to a direct effect of oxygen deprivation . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We also analyzed caspase 3 , 7 , 8 , 9 , Bcl 2 , Bax , Bid , and cytochrome C by Western analysis , and mitochondrial permeability and reactive oxygen species production by flow cytometry using mitosensor ( TM ) and DCFH DA , respectively . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| MATERIALS AND METHODS : The combined immunohistochemical expression levels of the proteins bax , bak , bad , bid , bcl 2 and bcl xl were evaluated by cluster and discriminant analysis . ^^^ RESULTS : Cluster analysis produced : a ) a low expression ( 69 / 79 cases ) and a high expression pro apoptotic cluster ( 10 / 79 cases ) for the combined expression levels of the pro apoptotic proteins bax , bak , bad and bid and b ) a low expression ( 37 / 76 cases ) and a high expression antiapoptotic cluster ( 39 / 76 cases ) for the combined expression levels of anti apoptotic proteins bcl 2 and bcl xl . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| By using rats exposed in utero to the anti androgenic compound flutamide ( 0 . 4 , 2 or 10 mg / kg per day ) , it has been shown that hypospermatogenesis in adult testes could be related to ( 1 ) a long term apoptosis in germ cells but not in somatic Leydig and Sertoli cells as evidenced by the TUNEL approach and ( 2 ) alterations in the mRNA and protein expression of pro ( Bax , Bak , Bid ) and anti apoptotic ( Bcl 2 , Bcl w ) members of the Bcl 2 family . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Whereas Bax or Bak were shown to be required for the proapoptotic effect of Bid , Bcl 2 was described to interfere with its action . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In turn , activated Bid , acting with pX induced Bax and Noxa , mediates the mitochondrial release of cytochrome c , resulting in the activation of caspase 9 and apoptosis . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The first alpha helix of Bax plays a necessary role in its ligand induced activation by the BH 3 only proteins Bid and PUMA . ^^^ We report that the first alpha helix ( Halpha 1 ) of Bax specifically interacts with the BH 3 domains of Bid and PUMA but not with that of Bad . ^^^ Inhibition of this interaction , by a peptide comprising Halpha 1 or by a mutation in this helix , prevents ligand induced activation of Bax by Bid , PUMA , or their BH 3 peptides . ^^^ Halpha 1 mutated Bax , which can mediate death induced by Bad or its BH 3 peptide , does not mediate that induced by Bid , PUMA , or their BH 3 peptides . ^^^ The response of Halpha 1 mutated Bax to Bid can be restored by a compensating mutation in Bid BH 3 . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| H / R can induce Bid and Bax mitochondrial translocation and cytochrome c release . ^^^ The expression of Bcl 10 ( L ) protected MLEC against H / R induced cell death by blocking Bax and Bid translocation and inhibiting mitochondrial cytochrome c release . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of the caspase 8 / Bid and Bax pathways in aspirin induced apoptosis in gastric cancer . ^^^ We showed that aspirin activated caspase 8 , caspase 9 and capase 3 , cleaved and translocated Bid , induced a conformational change in and translocation of Bax and cytochrome c release . ^^^ In conclusion , our results identify a role of caspase 8 / Bid and activation of Bax as a novel mechanism for aspirin induced apoptosis in gastric cancer . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The expression of the majority of proapoptotic genes in the DRG was also unaltered ( bax , bad , bid , bok , c Jun , p 38 , TNFR 1 , caspase 3 and NOS 2 ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Cardiolipin is important for the proapoptotic activity of Bcl 2 family members such as Bid , Bax and Bak , and modulation of its metabolism and translocation in mitochondrial membranes could potentially have a strong influence on apoptosis . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bands of Bid cleavage and Bax conformational change were detected by Western blot . ^^^ RESULTS : Death receptors , including TNF alpha and anti Fas antibody , induced hepatocytes apoptosis , Bax translocation and conformational change through activation of Bid , which caused Bax to be inserted into the mitochondrial membrane of hepatocytes . ^^^ The translocation and insertion of Bax were blocked when Bid was knocked out or blocked , and hepatocyte apoptosis was delayed or inhibited . ^^^ CONCLUSION : Hepatocyte apoptosis induced by death receptors is regulated by Bid and the translocation and insertion of Bax are also dependent on Bid . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In 22 / 36 patients with the pre treatment overexpression of Bax , Bak and Bid proteins , ALT induced a distinct ( more than 50 % from the baseline ) increase in the incidence of apoptosis after 24 h of in vitro treatment . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The cytosolic expression of Bid , Bax , and XIAP and release of cytochrome c and Smac / DIABLO were determined by immunoblotting . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Combined exposure to flavopiridol and HA 14 1 was associated with down regulation of Mcl 1 and Bcl xL , Bid cleavage , and mitochondrial translocation of Bax . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) induces programmed cell death through the caspase activation cascade and translocation of cleaved Bid ( tBid ) by the apical caspase 8 to mitochondria to induce oligomerization of multidomain Bax and Bak . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Treatment of the cells with 6ME caused activation of caspase 3 , 8 and 9 protease and subsequent proteolytic cleavage of poly ( ADP ribose ) polymerase . 6ME increased the expression of p 53 and Bax and decreased the expression of Bid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| MtDNA depleted cells show a 3 4 fold increased proapoptotic proteins ( Bax , BAD and Bid ) , markedly increased antiapoptotic Bcl 2 , and reduced processing of p 21 Bid to active tBid . ^^^ Inhibition of apoptosis closely correlated with sequestration of Bax , Bid and BAD in the mitochondrial inner membrane , increased Bcl 2 and Bcl 10 ( L ) , and inability to process p 21 Bid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Humanin binds and nullifies Bid activity by blocking its activation of Bax and Bak . ^^^ We show here that HN also interacts with the BH 3 only Bcl 2 / Bax family protein , Bid , as well as a truncated form of Bid ( tBid ) associated with protease mediated activation of this proapoptotic protein . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The potentiation of As2O3 provoked apoptosis involved the increased disruption of mitochondrial transmembrane potential , increased caspase 3 activation and cytochrome c release from mitochondria , increased Bax and Bid activation , and attenuation of 27 kDa heat shock protein ( HSP 27 ) expression ; the potentiation was prevented by Bcl 2 overexpression . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Specifically , we observe that Abeta significantly reduces expression of antiapoptotic Bcl w and Bcl 10 ( L ) , mildly affects expression of bim , Bcl 2 , and bax , but does not alter expression of bak , bad , bik , bid , or BNIP3 . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Loss of expression of proapoptotic proteins was observed as follows : Bak , 24 of 41 samples ( 59 % ) ; Bad , 21 of 41 samples ( 51 % ) ; Bid , 20 of 41 samples ( 49 % ) ; Bax , 14 of 41 samples ( 34 % ) ; and Bim / Bod , 2 of 41 samples ( 5 % ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Besides having this derepression function , the Bid and Bim peptides activated Bax directly and were the only BH 3 peptides tested that could potently induce cytochrome c release from mitochondria in cultured cells . ^^^ Furthermore , Bax activator molecules ( cleaved Bid protein and the Bim BH 3 peptide ) synergistically induced cytochrome c release when introduced into cells along with derepressor BH 3 peptides . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Stable overexpression of Bcl 2 in IM 9 cells ( IM 9 / Bcl 2 ) inhibited As ( 2 ) O ( 3 ) mediated Bax activation and apoptosis , and this inhibition could be partially averted by cell permeable Bid Bcl 2 homology ( BH ) 3 peptide . ^^^ Meanwhile , Bax conformational change and oligomerization induced by As ( 2 ) O ( 3 ) were not inhibited by the pancaspase inhibitor z VAD fmk , although Bid cleavage could be completely abolished . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Interestingly , apoptotic +SA cells showed a paradoxical decrease in mitochondrial levels of pro apoptotic proteins Bid , Bax , and Bad , and a corresponding increase in mitochondrial levels of anti apoptotic proteins , Bcl 2 and Bcl xL , suggesting that mitochondrial membrane stability and integrity might actually be enhanced for a limited period of time following acute tocotrienol exposure . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Moreover , a significant overexpression of mRNA encoding cytochrome c , and proapoptotic Bid and Bax , was seen in freshly isolated cells from MDS patients . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Indeed , bortezomib induced increases of Bik and / or Bim in multiple cell lines but not notably of two other BH 3 only proteins ( Puma and Bid ) nor other family members ( Bax , Bak , Bcl 2 , and Bcl xL ) . ^^^ |
|
| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Peptides derived from apoptotic Bax and Bid reproduce the poration activity of the parent full length proteins . ^^^ Bax and Bid are proapoptotic proteins of the Bcl 2 family that regulate the release of apoptogenic factors from mitochondria . ^^^ Here we report the poration activity of fragments from these two proteins , containing the first alpha helix of a colicinlike hydrophobic hairpin ( alpha helix 5 of Bax and alpha helix 6 of Bid ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Additionally , expression of the apoptosis regulating proteins Bax , Bak , Bid , Bcl w , Bcl 2 , XIAP and Mcl 1 was evaluated in B CLL lymphocytes . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Under its native state , Bid is a soluble cytosolic protein that undergoes proteolysis and yields a 15 kDa activated form tBid ( truncated Bid ) . tBid translocates to mitochondria and participates in cytochrome c efflux by a still unclear mechanism , some of them at least mediated by Bax . ^^^ In a second set of experiments , heterologous coexpression of tBid and Bax in wild type and CL less yeast strains showed that ( 1 ) tBid binding and the subsequent alteration of mitochondrial bioenergetics increased Bax induced cytochrome c release and ( 2 ) the absence of CL favors Bax effects independently of the presence of t Bid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| During the process of death receptor mediated apoptosis , Bid is cleaved by activated caspase 8 , and then cleaved Bid conveys apoptotic signals to the mitochondria by activating Bax / Bak . ^^^ D 609 did not interfere with activation of caspase 8 and cleavage of Bid , whereas it blocked cytochrome c release from the mitochondria by inhibiting the activation of Bax and Bak . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We further found that codonoposide 1c induces mitochondrial translocation of Bid from cytosol , reduction of cytosolic Bax , and cytochrome c release from mitochondria . ^^^ Taken together , our data indicate that codonoposide 1c is a potent inducer of apoptosis and facilates its activity via Bid cleavage and translocation to mitochondria , Bax reduction in cytosol , release of cytochrome c and Smac / DIABLO into the cytosol , and subsequently caspase activation , providing a potential mechanism for the cytotoxic activity of codonoposide 1c . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , Bcl xL was upregulated , and Bid activation or translocation , or conformational changes of Bax were not identified . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| On the other hand , no induction was observed of the pro apoptotic Bcl 2 family members Bax and Bid at any time from 3 to 24 h following PEDF addition . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In these models , both SAHA and m carboxycinnamic acid bis hydroxamide induced growth arrest and caspase mediated apoptosis and increased p 21 protein levels , retinoblastoma hypophosphorylation , BH 3 interacting domain death agonist cleavage , Bax up regulation , down regulation of Bcl 2 , A 1 , and Bcl 10 ( L ) expression , and cleavage of poly ( ADP ribose ) polymerase and caspase 8 , 9 , 3 , 7 , and 2 . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of the TNF R 1 receptor results in the cleavage of BID into truncated BID ( tBID ) , which translocates to the mitochondria and induces the activation of BAX or BAK . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Recent studies demonstrate that hypoxia / reoxygenation can activate death receptor and mitochondria dependent apoptotic pathways , involving Bid and Bax mitochondrial translocation and cytochrome c release . ^^^ FLIP protected MLEC against hypoxia / reoxygenation by blocking both caspase 8 / Bid and Bax / mitochondrial apoptotic pathways . ^^^ FLIP inhibited Bax activation in wild type and Bid ( / ) MLEC , indicating independence from the caspase 8 / Bid pathway . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the effect of 3 microM clavulone 2 was accompanied by the up regulation of Bax , down regulation of Mcl 1 , and cleavage of Bid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Moreover , leptin delayed the cleavage of Bid and Bax , the mitochondrial release of cytochrome c and second mitochondria derived activator of caspase , as well as the activation of both caspase 8 and caspase 3 in these cells . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| These results conclude that Cin triggers apoptosis in PLC / PRF / 5 cells could be through the activation of pro apoptotic Bcl 2 family ( Bax and Bid ) proteins and MAPK signaling pathway . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Antiapoptotic Bcl 2 , proapoptotic Bax and Bid , Smac , and cytochrome c oxidase ( COX 4 ) were analyzed by Western blot analyses from cytosol and mitochondrial fractions . ^^^ ASA downregulated Bcl 2 protein expression and induced Bax translocation into the mitochondria and cleavage of Bid . ^^^ Bax and Bid involvement in apoptosis regulation was dependent on caspase activation , because caspase 8 inhibition suppressed Bax translocation and Bid processing . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Proteins for Western blot analysis included Fas associated death domain ( FADD ) and caspase 8 for extrinsic pathway , as well as Bcl 2 , Bcl XL , Bax , Bad , Bid , Akt , p Akt , and caspase 9 for intrinsic pathway . ^^^ Levels of FADD , Bax , Bad , and Bid were substantially increased in the TA induced myopathy group , whereas Bcl 2 , Bcl XL , Akt , p Akt , and caspase 9 did not change between control and myopathy groups . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The results showed that MEM induced cell apoptosis involved up regulation of Fas and down regulation of Bcl 2 , DR 3 , DR 4 , TNFRI , and TNFRII in HepG 2 cells , while no changes on the levels of Bax , Bid , Bad , and Bak protein were observed . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In comparing model simulations with experimental results , we find the best agreement when Bcl 2 blocks the release of cytochrome c by binding to both Bax and truncated Bid instead of Bax , truncated Bid , or Bid alone . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Inhibitors of caspase 3 , 9 blocked the cytotoxicity of 10t , 12c CLA . 10t , 12c CLA also induced translocation of Bax protein into the mitochondrial membrane and cleavage of Bid protein . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax , Bcl 2 , Bcl XL , Bid , cytochrome c and PKCd were detected by Western blotting . ^^^ A decreased expression of Bid was noted , although no significant changes in Bax , Bcl 2 and Bcl XL expression were observed after the combined treatment . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The results showed that Bcl 2 family molecules , such as Bid , Bak , and Bax , are involved in the parthenolide induced apoptosis and that the defective expression of Bcl 10 ( L ) might contribute to the higher parthenolide sensitivity in the SCK cells than in the other adenomatous cholangiocarcinoma cells . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| The lethal concentration range is 25 50 micromol / l . 3 , 4 DGE results in Bax oligomerization , release of cytochrome c from mitochondria , activation of caspases 9 and 3 , and Bid proteolysis . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Results also displayed that PEITC induced apoptosis involves the up regulation of p 53 and Bax protein , down regulation of the XIAP , Bcl 2 , Bcl ( XL ) and Mcl 1 proteins , cleavage of Bid , and the release of cytochrome c and Smac / Diablo , which were accompanied by the activation of caspases 9 , 3 and 8 . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Activation of calcium independent phospholipase A ( iPLA ) in brain mitochondria and release of apoptogenic factors by BAX and truncated BID . ^^^ Cleaved or truncated BID ( tBID ) is known to oligomerize both BAK and BAX . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In contrast , proapoptotic proteins , such as BID , BAX , and BAK , induce Cyt c release independently of the mPT without lysing the MOM . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We showed that the proapoptotic proteins Bid and Bax as well as caspase 8 are involved in the initiation of this apoptotic pathway triggered by TNF alpha . ^^^ Furthermore , Bid and Bax translocation into mitochondria were already evidenced after 6 h . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Protein protein interactions within the Bcl 2 family are mediated by the helical BH 3 domain of proapoptotic family members , such as Bad , Bak , Bax , or Bid . ^^^ When added to the BH 3 domain derived from other proapoptotic proteins such as Bak , Bax , or Bid , these AEMs significantly increased the Bcl 2 binding affinity of these BH 3 peptides by promoting the helical structure . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In the early stages , both rapid and transient ROS generation triggered apoptosis via Fas activation and subsequent caspase 8 dependent Bid cleavage , as well as by calpain mediated mitochondrial Bax cleavage . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In cultured cells , gene deletion of Bax / Bak or Bid abrogated apoptosis induced by overexpression of caspase 2 . ^^^ Collectively , these results indicate that proteolytic activation of Bid and the subsequent induction of the mitochondrial apoptotic pathway through Bax / Bak is essential for apoptosis triggered by caspase 2 . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 family members Bax and Bid then form pores in the outer mitochondrial membrane and release intermembrane proteins , e . g . , apoptosis inducing factor ( AIF ) and endonuclease G , which then translocate to the nucleus and initiate chromatin condensation and DNA fragmentation , respectively . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Radiation did not affect proapoptotic proteins ( Bax and Bid ) , whereas H ( 2 ) O ( 2 ) increased accumulation of Bax in the mitochondrial membrane 2 h to 6 h after treatment , independently of the truncation of Bid by activated caspase 8 . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Up regulation of Bad and p 21 ; down regulation of Bcl 2 , Bcl XL , Bid , p 53 , and fatty acid synthase ; and cleavage of Bax were found in HMDB treated A 431 cells . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Engagement of death receptors such as tumor necrosis factor R 1 and Fas brings about the cleavage of cytosolic Bid to truncated Bid ( tBid ) , which translocates to mitochondria to activate Bax / Bak , resulting in the release of cytochrome c . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax and Bid , two proapoptotic Bcl 2 family members , inhibit homologous recombination , independently of apoptosis regulation . ^^^ In order to analyse the relationships between regulation of apoptosis and homologous recombination ( HR ) , we overexpressed proapoptotic Bax or only BH 3 Bid proteins or antiapoptotic Bcl 2 or Bcl XL , in hamster CHO cells or in SV 40 transformed human fibroblasts . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| After treatment with oridonin for 48 hr , the percentage of disruption of delta psi m gradually increased in a dose dependent manner along with marked changes of cell apoptosis , and necrotic cells increased remarkably after the cells were treated with oridonin for 72 hr ; Western blotting showed cleavage of the caspase 3 zymogen protein ( 32 kDa ) with the appearance of its 20 kDa subunit when apoptosis occurred ; expression of Bcl 2 and Bcl XL was downregulated remarkably while expression of Bax and Bid was upregulated concurrently after the cells were treated with oridonin for 24 hr . ^^^ We therefore conclude that oridonin has significant antiproliferation effects on HPB ALL cells by induction of apoptosis as well as directly causing cell necrosis and that oridonin induced apoptosis on HPB ALL cells is mainly related to the disruption of delta psi m and activation of caspase 3 as well as downregulation of anti apoptotic protein Bcl 2 , Bcl XL , and upregulation of pro apoptotic proteins Bax and Bid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bid , BH 3 interacting domain death agonist , was initially cloned based in its ability to interact with both Bcl 2 and Bax . ^^^ Bid , BH 3 interacting domain death agonist , was initially cloned based in its ability to interact with both Bcl 2 and Bax . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 , Bid , and Bad inhibit assembly sub stoichiometrically , whereas peptides from Bak and Bax promote tubulin polymerization at near stoichiometric concentrations . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We conclude that JNK dependent regulation of Bax is essential to mediate the apoptotic release of cytochrome c regardless of Bid and Bim activation . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| All tested thimidine containing PS ODN , irrespective of their primary sequences , reduced the expression of Bcl 2 protein and increased the levels of the proapoptotic molecules p 53 , Bid , Bax in CLL cells . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| These responses on UVB and / or sanguinarine treatments were associated with ( a ) decrease in Bcl 2 and Bcl 10 ( L ) and ( b ) increase in Bax , Bid , and Bak protein levels . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| We also found that gamma T 3 induced apoptotic cell death was accompanied by up regulation of Bax and a rise in the fragments of Bid and caspase 8 . ^^^ Moreover , these results suggest that Bax and Bid regulated apoptosis induction by gamma T3 . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis revealed that ISL increased the levels of membrane bound Fas ligand ( FasL ) , Fas , cleaved casapse 8 , truncated Bid ( tBid ) , Bax and Bad in DU 145 cells ( P < . 05 ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Apoptotic melanocytes , containing fragmented nucleus , show translocation of the proapoptotic proteins Bax and Bid from the cytosol to punctate mitochondrial like structures . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry was employed to examine the expression of the antiapoptotic proteins Bcl 2 and Bcl XL and the proapoptotic proteins Bad , Bak , Bax , Bid , Bim , and p 53 in 21 cases of gastric cancer . ^^^ For the proapoptotic members of the Bcl family , loss of protein expression was observed : Bid ( 14 / 21 cases ; 66 % ) , Bad ( 13 / 21 cases ; 61 % ) , Bax ( 12 / 21 cases ; 57 % ) , Bak ( 9 / 21 cases ; 42 % ) , and Bim ( 4 / 21 cases ; 19 % ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In addition , anandamide increased the level of Bax and caused degradation of full length Bid with the production of the active truncated form . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Among proapoptotic proteins , Bax expression was activated in H ( 2 ) S treated cells but not Bid , and the antiapoptotic proteins Bcl 10 ( L ) and Bcl 2 did not show any activation in pancreatic acinar cell apoptosis . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the mitochondrial apoptotic pathway amplified the calpain pathway by Bid and Bax interaction and Ca2+ translocation . ^^^ We have therefore concluded that the molecular mechanisms during EAC mediated proliferation inhibition in Hep 3B cells were due to : ( 1 ) apoptosis induction , ( 2 ) triggering of Ca2+ / calpain pathway , ( 3 ) disruption of mitochondrial function , and ( 4 ) apoptotic signaling being amplified by cross talk between the calpain / Bid / Bax and Ca2+ / mitochondrial apoptotic pathways . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Bax mediated apoptosis seems to be dependent on caspase 8 activation because the inhibition of caspase 8 prevented Bid processing and Bax translocation . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Using GFP biosensors and fluorescence deconvolution microscopy , we investigated mitochondrial morphology in relation to Bax and Bid activation in the HL 1 cardiac cell line . ^^^ Bid processing was caspase 8 dependent , and Bid translocation to mitochondria occurred after Bax translocation and clustering , and minutes before cell death . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Resveratrol treatment for LNCaP cells was also found to result in a significant ( a ) loss of mitochondrial membrane potential , ( b ) inhibition in the protein level of antiapoptotic Bcl 2 , and ( c ) increase in proapoptotic members of the Bcl 2 family , i . e . , Bax , Bak , Bid , and Bad . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Andro triggered a caspase 8 dependent Bid cleavage , followed by a series of sequential events including Bax conformational change and mitochondrial translocation , cytochrome c release from mitochondria , and activation of caspase 9 and 3 . ^^^ Inhibition of caspase 8 blocked Bid cleavage and Bax conformational change . ^^^ Consistently , knockdown of Bid protein using small interfering RNA ( siRNA ) technique suppressed Andro induced Bax conformational change and apoptosis . ^^^ In conclusion , the pro apoptotic Bcl 2 family members ( Bid and Bax ) are the key mediators in relaying the cell death signaling initiated by Andro from caspase 8 to mitochondria and then to downstream effector caspases , and eventually leading to apoptotic cell death . . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| In the type 1 cells , IETD prevented the translocation of Bax , the degradation of Bid and the accumulation of tBid . ^^^ In these cells in the presence of IETD , Bax translocated to the mitochondria , in the absence of any degradation of Bid or accumulation of tBid . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Calpain activation is followed by Bax and Bid cleavage , cytochrome c , apoptosis inducing factor , and endonuclease G release from mitochondria . ^^^ Apoptosis was reduced by Bax siRNA and Bid siRNA suggesting Bax and Bid degradation is apoptosis correlated . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| For pro apoptotic proteins , loss of expression was recorded as follows : Bad 25 % ( 14 / 54 ) , Bak 24 % ( 13 / 54 ) , Bax 42 % ( 23 / 54 ) , Bid 37 % ( 20 / 54 ) , Bim 18 % ( 10 / 54 ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| This applies to tumor suppressors of the Bax / Bcl 2 family ( Bax , Bad , Bid , Bim ) , several protein kinases ( Akt , ASK 1 , hexokinase ) , p 53 , NF kappaB , and nuclear orphan receptors such as TR3 / Nur77 . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , we treated isolated mitochondria with Bcl 2 antagonistic peptides ( derived from the BH 3 domain of Bax or Bid ) . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Moreover , sulindac sulfide activated caspases 3 and 8 , decreased the levels of Bax and Bid proteins , caused cleavage of PARP and increased the expressions of the bax and caspase 3 genes . ^^^ |
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| Interacting proteins: Q07812 and P55957 |
Pubmed |
SVM Score :0.0 |
| Here , we report that TRAIL signaling in LNCaP prostate cancer cells stalled at downstream of caspase 8 and BID cleavage , as indicated by the lack of Bax translocation into mitochondria , and no subsequent activation of the caspase 9 cascade . ^^^ |
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