Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.56091966
Bcl 2 also interacted with Bcl 10 L and Mcl 1 and with the dominant inhibitors Bax and Bcl 10 S . 0.56091966^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.52983877
The proapoptotic effect of BAX is negatively regulated by its binding with BCL 2 . 0.52983877^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.60231454
Furthermore , phosphorylated Bcl 2 remained complexed with Bax in Taxol treated cells undergoing apoptosis . 0.60231454^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.51298008
One potential mechanism is overexpression of death promoting Bax compared with antiapoptotic Bcl 2 in islets . 0.51298008^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.51643607
At the same time , SP 600125 increased the interaction of Bax with Bcl 2 after 2 days of reperfusion . 0.51643607^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.81596003
Interaction of Bcl 2 with Bax was not observed under any condition . 0.81596003^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.6511555
While Bik does not show overt homology to the BH 1 and BH 2 conserved domains characteristic of the Bcl 2 family , it does share a 9 amino acid domain ( BH 3 ) with Bax and Bak which may be a critical determinant for the death promoting activity of these proteins . . 0.6511555^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.51192099
Sequence similarities between 19K and Bcl 2 are largely restricted to short Bcl 2 homology ( BH ) domains that mediate interaction with Bax . 0.51192099^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.77507451
Bcl 2 forms protein protein homodimers with itself and heterodimers with Bax , and previous experiments have shown that point mutations in Bcl 2 can abrogate Bax binding while leaving homodimerization intact . 0.77507451^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.8264791
Bax interacted similarly with itself as with the apoptosis suppressing family members Bcl 2 and Bcl xL in quantitative two hybrid studies . 0.8264791^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.55670049
The pro apoptotic protein Bax can homodimerize with itself and heterodimerize with the anti apoptotic protein Bcl 2 , but the significance of these protein protein interactions remains unclear . 0.55670049^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.64732499
These changes resulted in a decreased association of Bcl 2 with immunoprecipitable Bax and an increased association of Bax with immunoprecipitable Bcl 2 in MCF 7 cells treated with bFGF . 0.64732499^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :1.0778396
Bcl 2 has been shown to heterodimerize with the death inducing protein Bax , but the mechanism of action of Bcl 2 is not fully understood . 1.0778396^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.59266283
To determine whether Bcl 2 and Bax , a protein which heterodimerizes with Bcl 2 , may control these epidermal functions , we investigated the expression of these two oncogenes in cultivated human keratinocytes and melanocytes from the same donors , respectively induced to differentiate and to produce melanin . 0.59266283^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.58028558
We show that the Bcl 2 Bax heterodimer is maintained , and no other protein associates stably in detectable amounts with Bcl 2 , Bax , or the heterodimer in anti Bcl 2 and anti Bax immunoprecipitates from normal cells and cells exposed to apoptotic stimuli . 0.58028558^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.62212938
Using green fluorescent protein ( GFP ) Bax and blue fluorescent protein ( BFP ) Bcl 2 fusion proteins coexpressed in the same cell , we demonstrate a direct interaction between Bcl 2 and Bax in individual mitochondria . 0.62212938^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.52436982
The Bax protein shares highly conserved domains with Bcl 2 , some of which are required for the formation of Bax Bcl 2 heterodimers . 0.52436982^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.60887521
Effects of hyperthermia at 42 . 5 degreesC for 6 h on cell survival , cell cycle progression , and the localization and expression levels of Bcl 2 and Bax , as well as the association between Bcl 2 and Bax in human lung cancer cells were investigated . 0.60887521^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.66860582
In the presence of IL 3 , constitutively expressed Bcl 2 was phosphorylated on serine residue ( s ) , and phosphorylated Bcl 2 lost its capacity to heterodimerize with Bax . 0.66860582^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.53266851
The interaction between Bcl 2 and Bax triggers apoptosis in BEC and acts as a cell rheostat in BEC hyperplasia and its involution after biliary decompression . . 0.53266851^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.87260414
Our results suggest that the BH 4 domain of Bcl 2 is critical for its heterodimerization with Bax and for exhibiting anti apoptotic activity . 0.87260414^^^ NH 2 terminal BH 4 domain of Bcl 2 is functional for heterodimerization with Bax and inhibition of apoptosis . 0.78986422^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.56320513
Immunoprecipitation of Bax demonstrated a decreased association of Bax with Bcl 2 in these cells . 0.56320513^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.50003865
Upon induction of apoptosis , Bax formed homo oligomers in the mitochondrial fraction with no evidence for cross linking to Bcl 2 or Bcl 10 ( L ) . 0.50003865^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.73547656
Bcl 2 located at the ER was shown to interfere with apoptosis induction by Bax , ceramides , ionising radiation , serum withdrawal and c myc expression . 0.73547656^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :1.0194249
In the present study the effect of nimesulide ( NIM ) , a specific COX 2 inhibitor , on apoptosis and interactions between BCL 2 family death promoters BAX and BID and BAX and VDAC 1 were examined in human colon adenocarcinoma COLO 205 cells . 1.0194249^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.97466019
Our data support a simple model mechanism by which Bcl 2 interacts with activated Bax during apoptosis in an effective manner to neutralize the proapoptotic activity of Bax . . 0.97466019^^^ We show that in vitro the Bcl 2 dimer can still interact with activated Bax as a larger oligomer . 0.87279948^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bax and bcl 2 is not significantly affected by either CD40L or sIg cross linking . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At least two family members , Bcl xs and Bax , act in opposition to Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Heterodimerization with Bax is required for Bcl 2 to repress cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the case of bcl 2 , which opposes bax function , mRNA levels were reduced in ML 1 cells after IR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of both WAF 1 and bax mRNA was induced by wt p 53 in both the ts p 53 and ts p53 / bcl 2 transfected cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Signalling through Fas / APO 1 did not down regulate Bcl 2 or induce its antagonists Bax and Bcl xS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect could not be explained by alterations in bcl 2 or bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the expression of Bcl 2 , Bax , and Fas proteins in myocytes was examined by immunocytochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also examined the expression of Bcl 2 and Bax on these lines , which were intimately related to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Stromal cells regulate bcl 2 and bax expression in pro B cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , the levels of Bax , another Bcl 2 family member implicated in apoptosis remained unaltered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Amongst the other genes presently characterized to be regulated by p 53 are BAX , a homologue of the BCL 2 gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It has become clear that the bcl genes ( more explicitly bcl 2 and bax ) are important apoptosis regulators . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition to Bax , Gfi 1 also represses Bak , another apoptosis promoting member of the Bcl 2 gene family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 mediated cell survival and growth inhibition are both antagonized by Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Anti Fas treatment resulted in decreased p 53 protein levels , while bcl 2 and bax protein levels remained unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fas , bcl 2 , or bax do not appear to be involved in this process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of lymphocyte apoptosis by a novel immunosuppressant FTY 720 : relation with Fas , Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By contrast , the expression of bcl 2 or bax was not significantly affected by these treatments . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , activated K ras up regulates bcl 2 but has no effect on bax or bcl 10 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By contrast , levels of Bcl 2 , and two other Bcl 2 family members , Bax and Bak , remained unaltered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of the bcl 2 family members , IFN gamma directly induced bak but notably not bax , which is activated by p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no changes observed in Bcl 2 protein or bax mRNA levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family members analyzed were Bcl 2 , Bcl 10 , Bax , Bad , Bak , A 1 , and Mcl 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , an antagonist of Bcl 2 , was brightly expressed even in the Bcl 2dim population . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression is not influenced by IL 7 or pre BR stimulation in either pro B or pre B cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
None of the cytokines tested caused a change in the levels of expression of Bcl 2 , Bax , Bcl 10 , or Bak proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , no significant difference in bcl 2 , bax , or p 53 expression was observed in SBM kidney compared with controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No changes in Bax or Bcl 2 protein level were noted after IL 3 withdrawal and / or activation of 5 Abl PTK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In Western blotting , Bax expression was obviously up regulated , but Bcl 2 remained almost constant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A relation of p 53 , bcl 2 , or bax protein levels to apoptosis in SUDHL 4 was not appreciated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bcl 2 and Bax , on the other hand , were not affected by herbimycin A pretreatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoreactive ratio of Bcl 2 to Bax significantly increased both in the striatum and cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The treatments did not significantly alter the levels of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of apoptosis associated genes : Fas ligand , bcl 2 and bax in the rat liver allograft . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 family members ( bax , bcl 10 ) was modulated by fotemustine , etoposide and cisplatin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl xL , but not bcl 2 and bax mRNA , was highly inducible within 3 h after stimulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a pro apoptotic member of the Bcl 2 protein family that resides in the outer mitochondrial membrane . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The function of BAX is countered by BCL 2 and BCL XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , BCL XL , BCL 2 , and BAX can form ion conductive pores in artificial membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a member of the Bcl 2 protein family with proapoptotic properties . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These regulatory effects were specific , since Bcl 2 and Bax protein levels were not similarly modulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Only scattered mononuclear cells were positive for bak , bax and bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIDS KS cells expressed fas , bcl 2 , and bcl xL genes but lacked fasL and bax gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both the macrophages and SMCs in the plaques expressed BAX , a proapoptotic protein of the BCL 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In yeast , Bax is lethal , and this activity is suppressed by Bcl xL , Bcl 2 , and A 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression on rat ischemic kidney . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we show by Western blotting that human neutrophils do not express Bcl 2 or Bcl 10 but constitutively express Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However the expression of apoptosis related genes such as Fas , bcl 2 and bax was not affected by indomethacin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Targeting can be instated by deleting the domain or by replacing the BAX transmembrane segment with that of BCL 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , the apoptosis suppressor Bcl 2 and promoter Bax were not changed with the curcumin treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX and related proteins encoded by the BCL 2 gene family are involved in the regulation of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast to Bax , the production of the apoptotic antagonist , Bcl 2 , remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of I / CB supernatant on Fas , Bax , and Bcl 2 gene transcription was evaluated by Northern blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BH 3 is required for BID to interact with BCL 2 and BAX , as well as for its function as a death agonist . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , TS1alphabeta survival is independent of Bcl 2 , Bcl 10 , or Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of Bax and Bcl 2 expression in p 53 immunopositive breast cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression remained relatively constant during thymineless stress in all cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bcl xL , bcl xS , and bax levels were unaffected by CBFbeta SMMHC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 437 mediated apoptosis is not accompanied by downregulation of bcl 2 or bcl XL or upregulation of bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of c myc , p 53 , p 21 , bax , and bcl 2 protein were assessed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the protein levels of Bcl 2 and Bax did not change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the expression of Bcl 2 and Bax was up regulated at 8 days after EDS administration . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax gene is a member of the Bcl 2 family that functions to regulate the programmed cell death process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Mcl 1 , and also Bax and Bak , but not p 53 were demonstrable in the tumor cells by immunostaining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No conclusions or trends could be drawn from the immunohistochemical studies of Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses found no difference in the levels of Bcl 2 and Bax proteins between the two groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , Bcl 2 , and Bax in Cd34+ cells recovering after chemotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Conversely , Bcl 2 and the Bcl 2 to Bax protein ratio increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a proapoptotic ion channel forming protein of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These genes included BMP 2 , BMP 4 , Msx 1 , Msx 2 , 5 ' members of Hox complexes , Bcl 2 , Bax , and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is the first member of the bcl 2 family shown to promote apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax conformational change is prevented by Bcl 2 and Bcl xL but not by caspase inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The quantity of Bax , Bcl 2 , Aogen , and AT 1 receptor in these cells was evaluated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Epstein Barr virus encodes a novel homolog of the bcl 2 oncogene that inhibits apoptosis and associates with Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BA enhances the levels of BAX and BCL 2 proteins but does not alter the levels of BCL xS or BCL xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Fas , FasL , bcl 2 , and bax was examined at the mRNA level and by means of immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bcl xL , or Bax was largely unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of Bcl 2 , Bcl xL , and Bax were not altered by cisplatin treatment and mitogen rescue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Mcl 1 and caspases 2 and 3 protein levels varied widely , whereas Bax expression was comparable among cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family members , Bcl 2 , Bcl xl , and Bax , appear not to be involved in this process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression levels of Bcl 2 and Bax remained unchanged following G Rh 2 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism of tumor induced DC apoptosis involved regulation of Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Only 300 U / ml IFNgamma treated cells overexpressed bax , whereas Bcl 2 and p 53 proteins were not modified . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , the levels of Bax protein remained unchanged , resulting in dysregulated Bcl 2 / Bax ratios . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and BAX expression were lower in Tu 2449 and P 497 than in SRB 10 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , the effect of p 53 seems to be independent from Bcl 2 and Bax protein level regulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bax , and fas oncogene expression were also evaluated by the APAAP method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NO / ONOO ( ) mediated apoptosis was associated with increased expression of Bax with no change in Bcl 2 mRNA abundance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
But , no detectable change was recognized in expression of c Jun , Fas , Fadd , Fap 1 , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bax , p21 / WAF 1 , and p 53 proteins were assessed semiquantitatively by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , recombinant CTGF showed no effect on Bax protein expression but significantly reduced Bcl 2 protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We determined the p 53 , Bcl 2 , Bax and CPP 32 protein levels by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast to Bax , a proapoptotic Bcl 2 gene , ANT 1 was unable to elicit a form of cell death in yeast . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , there was no significant change of Bcl 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IRS 2 but not IRS 3 binds to Bcl 2 , and IRS 1 associates with Bcl XL but not with Bax or Bik . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we observed that the expression of various caspases , bax and bcl 2 , was upregulated in cystic kidneys . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The neurotrophin survival dependence of peripheral neurons in vitro is regulated by the proapoptotic BCL 2 homolog BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : Bax is a member of the Bcl 2 family and induces apoptosis of mammalian cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HCl treatment up regulated Bax and Bak expression , down regulated Bcl 2 expression , and activated caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was accompanied by increased expression of p 53 and Bax and decreased expression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , neither the presence of proapoptotic Bax nor antiapoptotic Bcl 2 or Bcl XL affected tBid degradation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl xL , Bad , Bak , and Bax levels were not altered by either treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both types of cell death were reduced by genetic deletion of Bax or by virally mediated overexpression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , Bcl 2 , Bcl 10 ( L ) , and Bax levels were not affected by bisindolylmaleimide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Incubation of ACHN cells with ADR augmented the expression of Fas and p 53 , but not Bcl 2 , Bax , or caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax , as well as neuronal apoptosis in SDN POA , were observed on postnatal day 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We immunohistochemically examined the protein expression of Bcl 2 , Bcl XL , Mcl 1 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a pro apoptotic gene of the Bcl 2 family , has been shown to be involved in developmental neuronal death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
D 064 cells also spontaneously start to express Bax , while Bcl 2 is downregulated in parallel . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of a pro apoptotic protein , Bax , but not Bcl 2 , was increased by the serum deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
With hypergastrinemia , the expression of Bcl 2 and Bak was increased and Bax decreased in the middle of the gland . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Other members of the Bcl 2 family ( Bag 1 , Bak , Bax , and Bcl xL ) were not altered in expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Simultaneously , Bax , Bcl 2 and caspase 3 proteins appeared in many fibers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cocaine also produced a dose dependent decrease in Bcl 2 protein levels , but had no effect on Bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Granzyme B mediated cytochrome c release is regulated by the Bcl 2 family members bid and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DHA treatment decreased bcl 2 levels in association with apoptosis , whereas bax levels remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Medial VSMCs staining positive for oxLDL showed expression of BAX , a proapoptotic protein of the BCL 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treating T 24 cells with cisplatin enhanced the expression of bax but not bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 positive , Bax negative status of the peritubular myoid cells may be a strategy for cell survival . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These findings are correlated with modulations in bax , bcl 2 , bcl 10 ( L ) and p 21 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein levels of p 53 , p 21 ( waf1 / cip1 ) , c myc , bcl 2 and bax were detected by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whether embryos quality is related to the regulation of BCL 2 , Bax , and Fas expressions requires further study . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Meanwhile , levels of bax and bcl 2 expression were relatively constant upon temperature shift down in both cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of protein p 53 , Bcl 2 , and Bax were measured with Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect appeared to be independent of modulation of Fas , Bcl 2 , or Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , a suppressive effect of FK 506 on Bcl 2 production but not on Bax production was confirmed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting was used to identify changes in the anti and proapoptotic proteins Bcl 2 and Bax , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax did not differ significantly between the two study groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Unexpectedly , bcl 2 staining was sparse and bax staining predominated in majority of cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No changes in the expressions of TRAIL receptors ( DR 4 and DR 5 ) and Bcl 2 or Bax were found after treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 10 decreased expression of bcl 2 , bcl 10 ( L ) , and mcl 1 but not bax mRNA in monocytes stimulated with GM CSF . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of other members of the Bcl 2 family such as Bax , Bcl 10 ( L ) and Bak were not affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of Bcl 2 blocked cisplatin induced apoptosis and Bax translocation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This apoptosis is accompanied by decreased levels of Bcl 2 and increased levels of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 expression was absent in U 937 cells and BAX levels were identical between Neo and BCR ABL clones . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IN down regulated the level of bcl 2 mRNA without changing the level of bax mRNA in K 562 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No relationship was found between AI and labeling indices of Bcl 2 , Bcl 10 , Bax , p 53 , APO . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also measured the expression of the cell cycle mediators Bax , Bcl 2 , pRb , and mdm 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and other pro apoptotic Bcl 2 family `` killer proteins ' ' and their victim the mitochondrion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Doxorubicin treatment induced Bax expression and down regulated Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a mammalian proapoptotic member of the Bcl 2 family , can induce cell death when expressed in yeast or plant cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Also , we have measured the levels of Bcl 2 , Bcl 10 , and Bax in 20 cases of T ALL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial Bcl 2 and Bax were not altered with age . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using RT PCR , bax , bcl 2 and p 16 were not detected either . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , TIMP 1 upregulation inhibited expression of bax but had no effect on the expression of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SP immunohistological staining method was performed to detect the changes of expressions of p 53 , Bcl 2 and Bax gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mild hypothermia did not alter Bcl 2 and Bax expression , and caspase activation was not observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : Bax is a proapoptotic member of the bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed no detectable changes in the steady state levels of Bcl 2 , Bcl 10 ( L ) , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 ( L ) , Bax and p 53 protein expression was revealed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Co stainings with muscle actin , splicing factor ( SC 35 ) , PCNA , bax and bcl 2 were also performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cl IB MECA reduced the expression of endogenous Bcl 2 , whereas it did not affect the expression of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both cell lines express almost the same levels of FADD , RIP , c FLIP , FAP 1 , Bax , Bcl 2 and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The gene expression of Bax , Bcl 2 and Bcl xL was analysed by Northern blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , expression levels of antiapoptotic factor bcl 2 and apoptosis inducing factor bax were not affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated , by immunohistochemistry , the presence of Bcl 2 , c jun and bax in CAm . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a multidomain , proapoptotic member of the Bcl 2 family that is required for normal spermatogenesis in mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
With YCU N 861 cells , ATRA also caused a decrease in Bcl 2 and Bcl 10 ( L ) and an increase in Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family member Bfl 1 / A1 sequesters truncated bid to inhibit is collaboration with pro apoptotic Bak or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Most notable were the prevention of apoptotic decreases in BCL 2 levels and increases in mitochondrial BAX levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a proapoptotic member of the Bcl 2 family of proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No modifications in the steady state levels of Bcl 2 , Bax , Fas and Fas ligand were observed following infection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The biomarkers in the panel included cyclin A , cyclin D 1 , p 21 , p 27 , bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , other proteins including Bcl 2 , BAX and virus derived proteins may interact with the ANT to regulate the MPTP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
K 7 does not associate with Bax , but does bind to Bcl 2 via its putative BH 2 domain . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ANG 2 also upregulated the expression of Fas , FasL , and Bax and downregulated the expression of Bcl 2 in GECs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proteins studied were Bcl 2 , Bcl xL ( anti apoptotic ) , Bax , Bad , Bak , and Bcl xS ( pro apoptotic ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nr 13 is an anti apoptotic member of the Bcl 2 family previously shown to interact with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
POF obtained from LA treated rats showed no changes in Bcl 2 or Bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a death promoting member of the Bcl 2 family of genes that are intimately involved in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nevertheless , we have detected a strong induction of bax and bcl 2 gene products . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No significant variations in the levels of Bcl 2 , Bax and Bak proteins were noticed upon treatment with 2CdA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a protein of the Bcl 2 family that promotes apoptosis and functions as a tumor suppressor gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We measured bcl 2 , Bax and caspase 3 to determine the mechanisms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , CD44v6 , and nm 23 were evaluated by immunocytochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and bcl 2 decreased during reoxygenation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , the levels of Bcl 2 , Bax and Bcl xL were unchanged in imexon treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A similar pattern was observed with Bax immunostaining ; however , no difference was observed in Bcl 2 immunostaining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The combination induced Bax expression and Bcl 2 inhibition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These proteins include the death antagonists Bcl 2 and Bcl 10 ( L ) , and death agonists Bax and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BCL 2 family member BAX plays a critical role in regulating apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and Bax protein in the failing human heart an inhibitor or accelerator of apoptosis ? ] . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ATRA also decreased Bcl 2 and increased Bax and decreased VEGF . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis for Bax , Bcl 2 , cytochrome c , and p 53 were performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of BCL 2 and BAX in cyclosporine induced experimental renal fibrosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ethanol decreased Jurkat cell expression of Bcl 2 , whereas ethanol increased Jurkat cell expression of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of Bcl 2 , Bax and cytochrome c were determined by immunoblotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Endothelial cells incubated with IL 8 had higher levels of Bcl 10 ( L ) : Bcl 10 ( S ) and Bcl 2 : Bax ratios . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and bcl 2 levels were determined and tissue morphology was examined by light and transmission electron microscopy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a pro apoptotic member of Bcl 2 family proteins and is central to mitochondria dependent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No or only minor changes in Bcl 2 , Bcl XL and Bax levels were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Under the same conditions GM CSF up regulated production of BAX as well as Bcl 2 , Bcl XL , survivin , and XIAP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , there was no change in protein expression of FADD , FAP 1 , Bcl 2 , or Bax at 32 . 5 or 37 degrees C . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of Bcl 2 and Bax expression also correlates with the survival effect elicited by PrP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All cell lines express high levels of cyclin E , c Myc , Bcl 2 , Bax , Bcl 10 ( L ) , and Mcl 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the indole derivatives employed did not affect the levels of bcl 2 , bax and fasL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No changes were noted in the expression of mRNAs encoding Bcl 2 , Bcl xL , Bax , caspase 8 , caspase 3 , or GAPDH . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chronic hypoxia did not change Bax protein levels but significantly decreased Bcl 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the same way , BFA did not alter Bcl 2 , Bax and Mcl 1 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bcl xL , but not DNC 8 , inhibited TRAIL induced Bax activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of P 53 , cyclin D 1 , cyclin E , Bax , and Bcl 2 was performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CMS did not influence the apoptosis in myocytes from old rats ( TUNEL assay , Bcl 2 , Bax , or p 53 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , Bcl 2 and Bcl xL protect neurons from apoptosis , while Bax and Bcl xS may act as proapototic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We performed Northern blot analysis for bax , bcl 10 ( L ) and bcl 2 at both temperatures . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of the Rho / Rho kinase pathway did not affect p 53 , Bcl 2 , or Bax levels in HSCs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No change was detected in Bcl 2 , Bax , or Bcl xL proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULT : The proteins of FGF , Bcl 2 and Bax were mainly expressed in mitral cells in the olfactory bulb of the rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl xL proteins were not changed , although Bcl 2 proteins increased with troglitazone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the expression levels of Bcl 2 , Bax , Bak , and Mcl 1 were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , there were no differences in the number of apoptotic cells or Bax and Bcl 2 levels between WT and Smad 3 / OSE . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SL 11144 decreased Bcl 2 and increased Bax protein levels in MDA MB 231 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , it did not affect Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , ONCO DG 1 cells grown on the clinostat showed elevated amounts of Bax , but reduced quantities of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 was increased , whereas activated caspases and bax were decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CO also significantly reduced mRNA for proapoptotic Bax , while it up regulated anti apoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , there were no appreciable alterations in the levels of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis associated proteins bax , bcl 2 , p 53 and the COX 2 enzyme were investigated immunohistochemically . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Extra mitochondrial HSP 60 complexes with both bax and bak , but not with bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The decapsulation does not affect GAPDH , beta actin , Bcl 2 , or Bax gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the anti apoptotic protein , Bcl 2 , and the pro apoptotic protein , Bax , was analyzed by Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , Bak , Bid , and A 1 were used as indicators for activation of the mitochondrial pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While Bcl 2 , Bcl 10 ( L , ) , and Bax were not affected , Mcl 1 was induced by IL 6 in human esophageal carcinoma cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 : Bax transcript ratios were similarly elevated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bcl xL , and Bax in JPX 9 cells was assessed with Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proapoptotic Bcl 2 family members Bak and Bax play central and redundant roles in the regulation of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , it reduced the expression of Bcl 2 and Bcl XL proteins , whereas it had no effect on the level of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , expression of Bax and Bcl 2 ( mRNA and protein ) did not change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no significant changes in Bcl 2 , Bcl xl , and Bax protein expression after troglitazone treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression levels of c fos , c jun , Bcl 2 , and Bax were examined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein level of Bcl 2 was decreased dramatically , while Bax and p 53 were not significantly affected by allitridi . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In LNCaP cells , the Bax level was increased , and Bcl 2 and E2F1 levels were decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 mRNA and protein was down regulated , with no change in Bax or Bcl XL protein production . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In paraffin embedded sections , immunostaining for bax , bcl 2 , cytochrome c , and caspase 8 was done . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although the HA induced apoptosis was associated with Bax protein levels , negligible Bcl 2 reduction was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of Bax and Bcl 2 in gastric mucosa . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , cocaine induced a decrease in Bcl 2 protein levels , with no effect on Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic Bcl 2 and Bcl xL proteins were downregulated , whereas the proapoptotic Bax was upregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , it induced apoptosis , mediated by an increase in p 53 and bax ( expression of bcl 2 was unaffected ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , pro apoptotic Bax was overexpressed while anti apoptotic Bcl 2 and Bcl 10 ( L ) were suppressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , Bcl 2 , Bcl xL , Bax , and Bak localized in the mitochondria were detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a pro apoptotic member of Bcl 2 family proteins , plays a central role in mitochondria dependent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the distal segments BAX and BCL 2 expression levels were higher . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both ASA and indomethacin reduced the protein levels of Bcl 2 and Bcl xl , but upregulated those of Bax and Bcl xs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , Bax and Bcl 2 protein levels were similar in TO and control fetuses at all time points . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After ursolic acid treatment , the anti apoptotic Bcl 2 protein decreased and Bax expression was enhanced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Histopathology , immunohistochemistry , and Western blotting for COX 2 , Bax and Bcl 2 were performed in stomach tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Selenite induced apoptosis in LAPC 4 cells correlated with a decrease in the Bcl 2 : Bax expression ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas expression of Bax remained unaffected , in particular , GCV and also 5 FC caused a decline in the level of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a mammalian pro apoptotic member of the Bcl 2 family , induces apoptotic like cell death in Arabidopsis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a proapoptotic gene of the Bcl 2 family , plays a key role in regulating cell death in the nervous system . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Data on Bcl 2 and Bax were supported by immunofluorescence for Bcl 2 and activated Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax belongs to the Bcl 2 family and induces apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a major proapoptotic member of the Bcl 2 family and a molecule required for apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of BCL 2 mRNA and protein was down regulated , with no change in BAX or BCLxL protein production . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , Bcl 2 expression was down regulated while Bax protein level was up regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An early increase in Bcl 2 to Bax ratio with sildenafil treatment was also observed in myocytes after SI RO . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
YHT increased the amount of the anti apoptotic protein , Bcl 2 , and the pro apoptotic protein , Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bcl xL and ICAD was reduced time dependently , whereas the expression of Bax was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in survival was associated with an increase in expression of mRNA of bcl xl but not bcl 2 and bax ex vivo . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
E 6 inhibition of apoptosis was accompanied with prolonged expression of Bcl 2 and reduced elevation of Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family members Bak and Bax constitute a mitochondrial gateway for multiple death pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
They reduced the expression of Bax and Bcl 2 proteins , but not Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of apoptosis related proteins Bcl 2 , Bax , and Bcl XL were not modulated by boswellic acid acetate . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , quercetin did not affect Bax and Bcl 2 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the heart , this cytosolic HSP 60 complexes with Bax , Bak and Bcl XL , but not with Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Survivin AS ODN significantly decreased mRNA level of survivin , but not those of bax and bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was also shown that nicotine increases the mRNA level of bax and decreases that of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RPL 6 could upregulate Bcl 2 and downregulate Bax in cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bcl 2 and Bax proteins were observed by immunohistochemical staining methods . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and bcl 2 expression were assessed by Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , there was no significant change in any other mitochondrial membrane proteins , such as Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased Bax and unchanged Bcl 2 levels resulted in an increased Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The level of anti apoptotic protein Bcl 2 was decreased without any change in the pro apoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The samples were examined with HE stain , immunohistochemistry technique ( bcl 2 , bax ) and TUNEL dyeing . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We further demonstrate that there were no changes in Bcl 2 and Bax expression , two target genes regulated by p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SP immunohistochemistry was used to detect the expression of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , Fas and Fas Ligand , were assessed by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NPD 1 also upregulates the anti apoptotic proteins Bcl 2 and Bcl xL and decreases pro apoptotic Bax and Bad expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the up regulation of bax , and the down regulation of bcl 2 and bcl xL , was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the expression levels of protein Bcl 2 and Bax show no significant change in response to resveratrol treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Initially , histone deacetylase inhibitors increase Bax protein levels without affecting Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , we did not observe any changes in Bcl 2 , Bcl XL , or Bax expression induced by COX 2 or PGE 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
T cell and epithelial cell apoptosis , Fas , Bax , Bcl 2 , and p 53 were evaluated by flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HCA also induced expression of caspase 3 and Bax , but decreased Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no significant differences in total protein levels of caspase 3 , Bcl 2 , Bad , and Bax , among the groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This pathway is triggered by activation of pro apoptotic Bcl 2 family members such as Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 associated 10 protein ( BAX ) was also expressed in binucleate cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All of the four mouse MM cell lines examined expressed Bax , Bcl xL , c Myc , and caspase 3 but not Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dot blotting experiments showed that , in stress conditions , Bcl 2 protein level decreases while Bax is increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis involved c Jun NH 2 kinase activation , mitochondrial translocation of Bax , and reduction of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BH 3 only proteins of the Bcl 2 family initiate apoptosis through the activation of Bax like relatives . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No down regulation of bcl 2 expression , and no cleavage of Bax protein were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
E1B 19K is a BCL 2 homolog that binds and inactivates proapoptotic BAK and BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax protein expression were analyzed by Western immunoblotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical assessment of P 53 gene , Bcl 2 , Bax and microsatellite instability are of no predictive value . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A direct association between IL 10 , Bcl 2 , and Bax was detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No obvious alteration of bcl 2 protein level occurred , but bax was up regulated in these TZD 18 treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , z VAD fmk plus TNFalpha increased Bax expression without affecting Bcl 2 and cytochrome expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expressions were not changed by trimidox . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using the same method , we also studied the expression of the apoptosis related genes Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 protein levels were also altered differently in aged muscle , compared to young . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , expression of total Akt , Bax , and Bcl 2 did not differ between both groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 expression was decreased by Fas antibody , while Bax expression increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Renal function , structure , and immunohistochemistry for Bcl 2 , Bcl XL , and Bax were analyzed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PUMA interacts with anti apoptotic Bcl 2 and Bcl 10 ( L ) and is dependent on Bax to induce apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression in NFkappaB , Bcl 2 , and Bax was assessed by Western blotting analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot results showed a decrease in Bcl 2 and up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a major proapoptotic member of the Bcl 2 family that is required for apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our study also showed increased Bcl 2 protein expression and decreased Bax protein expression with TIMP 1 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ratio of Bcl 2 to Bax was much higher in the MAD 2 siRNA transfectants compared with the SGC7901 / psilencer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Matrine did not alter the level of bcl 2 and bcl xL as well as bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activated caspase 3 and the apoptosis regulating proteins Bax , Bcl 2 , Mdm 2 , and p 53 were analyzed by immunostaining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bcl XL and Bax proteins was investigated using immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Involvement of Bax , Bcl 2 , Ca2+ and caspase 3 in capsaicin induced apoptosis of human leukemia HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , MMP 2 inhibition reduced Bax expression and caspase 3 activity , as well as increasing Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No differences were found in the regulation of Bax and Bcl 2 between groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify this , we investigated the expression of BCL 2 and BAX , and the effect of BCL 2 overexpression on activin A induced apoptosis in B cell hybridoma cell lines . ^^^ The activin A sensitive cell lines expressed BAX but not BCL 2 and that activin A did not increase BAX levels . ^^^ Thus , activin A has been shown to induce apoptosis by a BCL 2 inhibitable mechanism without activating BAX . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , the expression of bcl 2 , bcl 10 , bax and IL 1 beta converting enzyme did not correlate with susceptibility to anti IgM induced PCD . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX , a heterodimeric partner of BCL 2 , counters BCL 2 and promotes apoptosis in gain of function experiments . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Genes such as bcl 2 and bax have been implicated in the direct control of cell death , while cellular immediate early genes ( cIEGs ) , such as c fos and c jun have been repeatedly associated with neuronal degeneration . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax are homologous proteins which can heterodimerize with each other . ^^^ These proteins have opposing effects on cell survival when overexpressed in cells , with Bcl 2 blocking and Bax promoting apoptosis . ^^^ Northern blot analysis of BCL 2 transfected and control transfected Jurkat and 697 leukemia cells revealed no Bcl 2 induced alterations in the steady state levels of BAX mRNAs . ^^^ In contrast , L [ 35S ] methionine pulse chase analysis indicated a marked increase in the half life ( t1 / 2 ) of the p21Bax protein in BCL 2 transfected 697 cells compared to control transfected cells ( t1 / 2 > 24 h versus approximately 4 h ) , whereas the rate of Bax degradation was unaltered in Bcl 2 transfected CEM cells . ^^^ The results demonstrate that levels of the proapoptotic p21Bax protein can be post translationally regulated by Bcl 2 , probably in a tissue specific fashion , and suggest the existence of a feedback mechanism that may help to maintain the ratio of Bcl 2 to Bax protein in physiologically appropriate ranges . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A family of Bcl 2 related genes is emerging that includes Bax , a conserved homolog that heterodimerizes in vivo with Bcl 2 and promotes cell death . ^^^ The ratio of family members , such as Bcl 2 / Bax , determines the survival or death of cells following an apoptotic stimulus . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BAX gene is a member of the Bcl 2 gene family ; it encodes a 21 kDa protein whose association with Bcl 2 is believed to play a critical role in regulating apoptosis . ^^^ Unlike two previously described variant forms of Bax alpha ( Bax beta and Bax tau ) , Bax delta retains the functionally critical C terminal membrane anchor region as well as the Bcl 2 homology 1 and 2 ( BH 1 and BH 2 ) domains . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using LexA Bcl 2 as bait , we find that the cellular prion protein ( PrP ) interacts with Bcl 2 , but not Bax , in the yeast two hybrid system . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and its relatives bcl 10 and bax encode intracellular membrane bound proteins that share homology in three domains with a wider family of viral and cellular proteins . ^^^ High levels of Bax or of a smaller Bcl 10 variant antagonize the survival function of Bcl 2 . ^^^ The mechanism by which Bcl 2 promotes cell survival remains unknown , but it appears to require association with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In MCF 7 cells that harbor wild type p 53 , AHPN induced G0 / G1 arrest and apoptosis was accompanied by p 53 independent regulation of WAF1 / CIP1 as well as bax mRNA levels while bcl 2 mRNA levels were decreased . ^^^ In MDA MB 231 cells which possess a mutant p 53 , AHPN mediated G0 / G1 arrest and apoptosis was also associated with a concomitant up regulation of WAF1 / CIP1 mRNA while these cells did not express bax or bcl 2 messages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax protein that heterodimerizes with Bcl 2 and promotes cell death is expressed in MCF 7 cells grown in the presence of estrogen and is unaffected by culture in estrogen free medium . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Multiple Bcl 2 family members demonstrate selective dimerizations with Bax . ^^^ BH 1 and BH 2 domains of Bcl 2 were required for it to heterodimerize with Bax and to repress apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bcl 10 , and Bax after T cell activation and IL 2 withdrawal . ^^^ Bcl 2 , bcl 10 , and bax genes code for proteins that affect the susceptibility of cells to apoptosis . ^^^ In general , the expression of bcl 2 or bcl 10 inhibits apoptosis while bax promotes apoptosis . ^^^ Bcl 2 and Bax proteins vary coordinately , but Bcl 10 varies independently : Bcl 2 and Bax are higher in splenic T cells than in thymocytes , and their levels increase even more after T cell activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoprecipitation indicated that Bcl xL , like Bcl 2 , heterodimerized with the death promoting molecule Bax in thymocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These include Bax that heterodimerizes with Bcl 2 and , when overexpressed , counteracts Bcl 2 . ^^^ Bax contains two highly conserved regions of sequence homology with Bcl 2 , referred to as Bcl 2 homology 1 and 2 ( BH 1 and BH 2 ) domains . ^^^ Site directed mutagenesis studies have revealed that both domains are not only novel dimerization motifs for the interaction of Bax with Bcl 2 but also crucial for the survival activity of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of bcl 2 , bcl XL and bax in the control of apoptosis by hematopoietic cytokines and dexamethasone . ^^^ Treatment of M 1 myeloid leukemic cells with interleukin 6 ( IL 6 ) or dexamethasone ( DEX ) , both of which induce differentiation in these cells , down regulated expression of the apoptosis suppressing gene bcl 2 and the apoptosis promoting gene bax but up regulated expression of the apoptosis suppressing gene bcl XL . ^^^ Another myeloid leukemia that shows barely detectable expression of bcl 2 also showed up regulated expression of bcl XL but no change in bax after induction of differentiation with granulocyte macrophage colony stimulating factor , and this reduced cell susceptibility to induction of apoptosis by Adriamycin or cycloheximide . ^^^ The results indicate that the related apoptosis regulating genes bcl 2 , bcl XL , and bax are differently regulated and that up regulation of bcl XL expression may compensate for down regulation of bcl 2 in the balance between genes that control apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a homologue of Bcl 2 that promotes apoptosis . ^^^ Bax immunostaining was not significantly correlated with tumor histology , S phase fraction , aneuploidy , p 53 HER2 , or cathepsin D , but was positively associated with Bcl 2 ( P = 0 . 005 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Up regulation of bax and down regulation of bcl 2 is associated with kainate induced apoptosis in mouse brain . ^^^ The down regulation of bcl 2 , which promotes cell survival , and the up regulation of bax , which promotes programmed cell death , may have functional significance in kainate mediated excitotoxicity and in the selective vulnerability of specific brain regions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Second , downstream effectors of p 53 have been identified including p21Waf 1 / Cip 1 , an inhibitor of cyclin dependent kinases , and bax , a dominant negative inhibitor of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There are no cytokine related changes in Bcl 2 , Bax , or Bcl 10 protein levels that could account for the modulation of G 1 arrest versus apoptosis by growth factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human bcl 2 family of genes including bcl 2 , bcl 10 l , bcl 10 s , and bax has been shown to be functionally involved in apoptosis . ^^^ We applied a yeast two hybrid system to demonstrate that bcl 2 , bcl 10 l , bcl 10 s , and bax proteins can interact with each other directly . ^^^ All bcl 2 family members except bax were shown to be capable of homo interactions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of Bax alpha precedes apoptosis in a human B lymphoma cell line : potential role of the bcl 2 gene family in surface IgM mediated apoptosis . ^^^ We provide evidence that up regulation of Bax alpha and the resulting imbalance of Bcl 2 / Bax might be a major regulator of sIg mediated apoptosis . ^^^ We therefore propose a potential role of the novel bcl 2 gene family members bcl 10 and bax in surface IgM triggered apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and some of its homologues inhibit apoptosis , whereas other family members , such as Bax , will accelerate apoptosis under certain conditions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although the levels of Bcl 2 protein and Bax mRNA appear unaltered by the activation of p 53 , within 24 h the majority of cells undergo apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A related protein that promotes cell death , Bax , wrestles with Bcl 2 through conserved motifs , BH 1 and BH 2 , establishing a set point for these deaths . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , it was observed that a spectrum of p 53 target genes implicated in p 53 induced growth suppression and apoptosis were similarly regulated , either induced ( gadd 45 , waf 1 , mdm 2 , and bax ) or suppressed ( c myc and bcl 2 ) , after activation of wt p 53 function in M1p53 and M1p53 / bcl 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Identification of conserved domains important for homodimerization with Bcl 2 and heterodimerization with Bax . ^^^ The Bcl 2 protein is a suppressor of programmed cell death that homodimerizes with itself and forms heterodimers with a homologous protein Bax , a promoter of cell death . ^^^ Expression of Bax in Saccharomyces cerevisiae as a membrane bound fusion protein results in a lethal phenotype that is suppressible by co expression of Bcl 2 . ^^^ Functional analysis of deletion mutants of human Bcl 2 in yeast demonstrated the presence of at least three conserved domains that are required to suppress Bax mediated cytotoxicity , termed domains A ( amino acids 11 33 ) , B ( amino acids 138 154 ) , and C ( amino acids 188 196 ) . ^^^ In vitro binding experiments using GST Bcl 2 fusion proteins demonstrated that Bcl 2 ( delta B ) and Bcl 2 ( delta C ) deletion mutants had a markedly impaired ability to heterodimerize with Bax but retained the ability to homodimerize with wild type Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In view of the recent identification of Bax as a protein that antagonizes the anti apoptotic function of Bcl 2 , we sought to determine whether the inability of transfected bcl 2 to protect against myeloma cell apoptosis might simply be due to insufficient levels of Bcl 2 protein produced to counteract this inhibitor . ^^^ We show here that high level expression of an exogenous bcl 2 gene , introduced into IL 6 dependent B 9 myeloma cells via retroviral or bovine papilloma virus based vectors , is indeed able to suppress apoptotic death following cytokine deprivation , with the extent of protection provided correlating with the amount of Bcl 2 protein synthesized in relation to the amount of endogenous Bax protein present in the cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We utilized a reverse transcription PCR method to examine the effect of estrogen on the expression of mRNA for Bcl 2 and Bax , two modulatory proteins in the apoptotic pathway , in human breast cancer cell line MCF 7 . ^^^ Although the relative bax mRNA levels remained unchanged , the changes in bcl 2 mRNA level occurred in a time and concentration dependent fashion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , expression of bax , an antagonistic bcl 2 homologue , was not affected by oncogenic Ras or IL 3 deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of members of the bcl 2 gene family in the immature rat ovary : equine chorionic gonadotropin mediated inhibition of granulosa cell apoptosis is associated with decreased bax and constitutive bcl 2 and bcl xlong messenger ribonucleic acid levels . ^^^ Herein we have characterized changes in the expression of the bcl 2 protooncogene ( an inhibitor of apoptosis ) , the bax gene ( an inducer of apoptosis ) , and the bcl 10 gene ( which encodes both bcl xlong , an inhibitor of apoptosis , and bcl xshort , an inducer of apoptosis ) during gonadotropin stimulated follicular development in vivo and during atresia of antral follicles incubated in vitro . ^^^ Complementary DNA fragments corresponding to rat bcl 2 , rat bax , and rat bcl 10 coding sequences were obtained by the reverse transcription polymerase chain reaction ( RT PCR ) technique using total RNA prepared from immature rat ovaries . ^^^ Northern blot analysis of steady state bcl 2 , bax , and bcl 10 messenger RNA ( mRNA ) levels in total RNA prepared from ovaries of immature rats before and after in vivo priming with 10 IU equine CG ( eCG ) revealed that eCG induced follicular growth and survival were associated with a relatively constitutive level of bcl 2 and bcl 10 expression but markedly reduced levels of bax mRNA ( 29 + / 5 % of saline treated control animals ) . ^^^ The eCG mediated decrease in bax expression , coupled with a maintenance of bcl 2 and bcl xlong mRNA levels , were associated with a pronounced reduction in the extent of granulosa cell apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was further determined by Northern blot analysis that this higher sensitivity of B myb overexpressing clones was not due to a change in the expression of TGF beta receptor type 1 or in the kinetics of the regulation of c myc , c myb , bcl 2 , and / or bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bad , a heterodimeric partner for Bcl XL and Bcl 2 , displaces Bax and promotes cell death . ^^^ Bad selectively dimerized with Bcl xL as well as Bcl 2 , but not with Bax , Bcl xs , Mcl 1 , A 1 , or itself . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some members of the BCL 2 family ( bcl 2 alpha , bcl xL ) inhibit apoptosis , whereas some other ( Bax , Bclxs ) induce it . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of bcl 2 and bax mRNA in axotomized dorsal root ganglia of young and adult rats . ^^^ Bcl 2 and Bax have recently been identified as putative repressor and effector proteins respectively , in the cell death program of growth factor deprived haematopoietic cell lines . ^^^ In the present in vivo study the expression of bcl 2 and bax mRNA has been investigated in dorsal root ganglia of young and adult rats using polymerase chain reaction . ^^^ The age dependent decline in the ratio of bcl 2 to bax gene products may explain the greater susceptibility of immature neurons to apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have previously demonstrated that the gonadotropin mediated inhibition of apoptosis in ovarian granulosa cells is linked to changes in the expression of several cell death related genes , including members of the bcl 2 gene family ( bcl 2 , bax , and bcl 10 ) . ^^^ Recently , the product of the p 53 tumor suppressor gene , a protein reported to play a critical role in regulating cell proliferation and death , has been shown to directly modulate the transcriptional activity of the bcl 2 and bax genes . ^^^ Based on our identification of a potential role for bcl 2 related factors in regulating granulosa cell apoptosis and the reported function of p 53 as a regulator of bcl 2 and bax gene transcription in extragonadal cells , the present studies were conducted to determine whether the p 53 and WT 1 genes are expressed and gonadotropin regulated in the rat ovary and to investigate whether granulosa cell apoptosis is linked to elevated levels of tumor suppressor gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the bcl 2 gene family in normal and malignant breast tissue : low bax alpha expression in tumor cells correlates with resistance towards apoptosis . ^^^ We have studied the expression of the apoptosis regulating genes bcl 2 , bcl 10 , bax and APO 1 / fas ( CD 95 ) in human breast cancer . ^^^ We therefore propose that dysregulation of apoptosis contributes to the pathogenesis of breast cancer , at least in part , due to an imbalance between anti apoptosis genes ( such as bcl 2 / bcl 10 ) and apoptosis promoting genes ( bax ) . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential regulation of c fos , fosB , c jun , junB , bcl 2 and bax expression in rat skin following single or chronic ultraviolet irradiation and in vivo modulation by antisense oligodeoxynucleotide superfusion . ^^^ To obtain information in vivo about a possible relationship between u . v . induced proto oncogene expression and cellular alterations , we have analysed the expression of c fos , fosB , c jun , junB , bcl 2 and bax in rat epidermis after single and chronic u . v . irradiation . ^^^ Single u . v . irradiation causes a rapid and sustained increase in c jun , junB and c fos mRNA and a decline in bcl 2 transcripts , whereas expression of bax remained unchanged . c Fos and c Jun immunoreactivity was localized throughout the epidermal cell layers 1 . 5 h after single irradiation , but restricted to basal cells at 48 h suggesting an involvement in both u . v . induced apoptosis and hyperproliferation . 48 h after chronic exposure a significantly higher induction and a totally different pattern of epidermal proto oncogene expression was detectable which may be associated with malignancy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical determination of in vivo distribution of Bax , a dominant inhibitor of Bcl 2 . ^^^ The cell survival promoting activity of this protein is opposed by Bax , a homologous protein that forms heterodimers with Bcl 2 and accelerates rates of cell death . ^^^ The expression of bax was more widespread than bcl 2 . ^^^ For example , Bax immunoreactivity was present in the hepatocytes of the liver , the exocrine pancreas , and the renal tubule epithelial cells whereas Bcl 2 was absent from these tissues . ^^^ Both the Bax and Bcl 2 proteins were present in several epithelia examined , including the small intestines , colon , breast , prostate , respiratory tract , and skin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / Bax : a rheostat that regulates an anti oxidant pathway and cell death . ^^^ A family of bcl 2 related genes is emerging that includes Bax , a conserved homolog that heterodimerizes in vivo with bcl 2 . ^^^ A pre set ratio of Bcl 2 / Bax appears to determine the survival or death of cells following an apoptotic stimulus . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sequence analysis of the BHRF 1 protein disclosed similarity with the recently described bcl 2 homologues bcl 10 ( 32 % ) and bax ( 34 % ) over the carboxyl portion , with several domains of complete identity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recently , both Bcl 2 , which promotes cell survival , and Bax , which promotes cell death , have been implicated as major players in the control of apoptotic pathways , and it has been suggested that the ratio of Bcl 2 and Bax protein controls the relative susceptibility of cells to death stimuli . ^^^ Both p 53 and TGF beta 1 down regulated endogenous Bcl 2 expression , but only p 53 up regulated Bax expression , where bax has been identified as a p 53 immediate early response gene . ^^^ Thus , the p 53 mediated up regulation of Bax may provide at least a partial explanation for the more rapid rate of apoptosis induced by p 53 compared to by TGF beta 1 , as well as for the ineffectiveness of ectopoic Bcl 2 to abrogate p 53 mediated apoptosis . ^^^ These findings provide first insights to the molecular mechanisms which mediate p 53 induced apoptosis , identifying bax and bcl 2 as p 53 regulated genes , and serve as a paradigm of how the intracellular balance of Bcl 2 to Bax is differentially altered by distinct death stimuli . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor suppressor p 53 is a regulator of bcl 2 and bax gene expression in vitro and in vivo . ^^^ Here we demonstrate that a temperature sensitive p 53 induces temperature dependent decreases in the expression of the apoptosis suppressing gene bcl 2 in the murine leukemia cell M 1 , while simultaneously stimulating increases in the expression of bax , a gene which encodes a dominant inhibitor of the Bcl 2 protein . ^^^ Mice deficient in p 53 exhibit increases in Bcl 2 and decreases in Bax protein levels in several tissues as determined by immunohistochemical and immunoblot methods . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When the predicted transmembrane domain of the Bcl 2 signal anchor was replaced with that of the signal anchor of the yeast outer mitochondrial membrane protein , Mas70p , the Bcl 2 / Mas70p hybrid was found to be very similar to Bcl 2 in its distribution within transfected KB cells , in its ability to heterodimerize with Bax , and in its ability to suppress apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 heterodimerizes in vivo with a conserved homolog , Bax , that accelerates programmed cell death . ^^^ We identified an associated 21 kd protein partner , Bax , that has extensive amino acid homology with Bcl 2 , focused within highly conserved domains 1 and 2 . ^^^ Bax homodimerizes and forms heterodimers with Bcl 2 in vivo . ^^^ Overexpressed Bax also counters the death repressor activity of Bcl 2 . ^^^ These data suggest a model in which the ratio of Bcl 2 to Bax determines survival or death following an apoptotic stimulus . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we have investigated the possibility that the three recently identified Bcl 2 homologues , Bax , Bcl 10 , and Mcl 1 , could be involved instead . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we demonstrate that exposure of mice to 800 cGy of gamma radiation results in rapid elevations in the levels of the Bax protein , a pro apoptotic member of the Bcl 2 protein family , in lymphoid cells and SIEs . gamma Radiation induced increase in Bax protein were evident within 2 h and persisted for at least 24 h , as determined by immunoblotting and immunohistochemical assays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced by wild type p 53 but not by serum deprivation was accompanied by G 1 cell cycle arrest and increased expression of the Bcl 2 antagonist Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis of cellular expression of Bcl 2 family proteins , Bcl 2 , Bax , Bcl 10 and Mcl 1 , in human peripheral blood and lymphoid tissues . ^^^ Several homologues of the bcl 2 gene , such as bax , bcl 10 or mcl 1 , have recently been identified . ^^^ Like Bcl 2 , both Bcl XL and Mcl 1 appear to function as repressors of apoptotic cell death , whereas Bax facilitates it , indicating possible interactions among them in the control of cellular survival . ^^^ Sole expression of Bax , but not other members of the Bcl 2 family , was observed on neutrophils , seemingly reflecting their shortest life span among blood leukocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in the frontoparietal cortex of the rat following cardiac arrest . ^^^ In the present study , involvement of Bcl 2 and Bax in these phenomena was investigated . ^^^ In the frontoparietal cortex , both bcl 2 and bax mRNA levels were significantly increased 1 , 3 , 7 , 14 , and 28 days postresuscitation . bax mRNA levels continued to be high 45 days postcardiac arrest , whereas bcl 2 mRNA levels were returned to control levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
With better understanding of the functional significance of these protein protein interactions involving Bcl 2 , Bax , and other members of the Bcl 2 protein family and with insights into the structural details of these interactions , it may eventually be possible to develop novel pharmacological agents that improve tumor responses to currently available anticancer drugs and that have other clinically relevant uses as well . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Residues 50 78 of Bax containing a conserved region designated Bcl 2 homology region 3 ( BH 3 ) were sufficient for specific binding to both the E1B 19K and Bcl 2 proteins . ^^^ The Bax E1B 19K interaction was detectable in vitro and in lysates from mammalian cells , and Bax expression antagonized E1B 19K protein function . bax mRNA and protein levels were p 53 inducible with kinetics identical to that of p21 / Waf 1 / Cip 1 , and E1B 19K and Bcl 2 expression did not affect Bax or p21 / Waf 1 / Cip 1 accumulation . ^^^ In cells where p 53 was mutant , Bax expression induced apoptosis , suggesting that Bax was sufficient for apoptosis , and acted downstream of p 53 . p 53 may simultaneously activate the transcription of genes required for both growth arrest ( p21 / Waf 1 / Cip 1 ) and death ( bax ) , and E1B 19K and Bcl 2 may act distally and function through interaction with and antagonism of Bax to prevent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that the parental HL 60 cells expressed both Bcl 2 and Bax , two factors known to be intimately involved in the control of apoptosis . ^^^ Surprisingly , HCW 2 cells no longer expressed Bcl 2 protein and paradoxically contained Bax protein at a level that was approximately 50 fold higher than in HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of mitochondria and C terminal membrane anchor of Bcl 2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae . ^^^ In mammalian cells , the Bcl 2 and Bcl 10 ( L ) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process . ^^^ Recently published data suggest that expression of the human Bax alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can be overcome by co expressing Bcl 2 or Bcl 10 ( L ) . ^^^ Our results indicate that the process involving Bax induced growth inhibition followed by possible lethality , and the rescuing effect of Bcl 2 and Bcl 10 ( L ) is linked to yeast mitochondrial function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / bax mRNA expression ratio as prognostic factor in low grade urinary bladder cancer . ^^^ Apoptotic cell death represents an important mechanism for the precise regulation of cell numbers , and a defence mechanism against tumoral cell . bcl 2 and bax genes are known to be involved in the control of apoptotic pathways ; in particular , the ratio between bcl 2 and bax represents a cell rheostat that is able to predict a cell ' s response toward life or death to an apoptotic stimulus . ^^^ In the present study we investigated the role of bcl 2 and bax gene expression in a panel of 37 low grade tumours of the urinary bladder , and correlated the expression of these genes to the prognosis of patients in a follow up of more than one year . ^^^ We found that levels of bax expression higher than bcl 2 in bladder tumours well correlates to a better outcome for patients . ^^^ Early relapses are much more frequently observed in those patients whose tumours express more bcl 2 than bax mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax and p 53 expression in B CLL in relation to in vitro survival and clinical progression . ^^^ We have now analyzed the in vitro survival of B CLL cells in relation to Bcl 2 , Bax alpha and p 53 expression and compared this with the clinical progression of the disease . ^^^ However , since Bax alpha expression was lower in progressive than in non progressive patients , the Bcl 2 / Bax alpha ratio at the mRNA level was significantly higher in the progressive group . ^^^ Our data suggest that the Bcl 2 / Bax alpha ratio is important for the regulation of B CLL cell survival , that p 53 over expression in progressive B CLL is the result of post transcriptional modifications and that a directed PKA activation may potentiate the cytolytic effect of glucocorticoids in vivo . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interleukin 2 receptor common gamma chain signaling cytokines regulate activated T cell apoptosis in response to growth factor withdrawal : selective induction of anti apoptotic ( bcl 2 , bcl xL ) but not pro apoptotic ( bax , bcl xS ) gene expression . ^^^ This rescue involves the induction of the anti apoptosis genes bcl 2 and bcl xL ) , but causes little change in expression of bax and bcl xS , which promote apoptosis . ^^^ Thus , when proliferation is blocked , the common gamma chain cytokines still induce up regulation of bcl 2 relative to bax and retard apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IGF 1 or insulin suppresses ICE mediated cell death without affecting the expression levels of Bcl 2 , Bcl 10 , or Bax . ^^^ Taken together , these results indicate that ICE is activated by growth factor deprivation , and IGF 1 is able to suppress ICE mediated cell death through a mechanism independent of the expression of Bcl 2 , Bcl 10 , or Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypersensitivity of human testicular tumors to etoposide induced apoptosis is associated with functional p 53 and a high Bax : Bcl 2 ratio . ^^^ Second , the testicular tumor lines expressed relatively high levels of the apoptosis promoting protein Bax , but there was no expression of the suppressor of apoptosis Bcl 2 . ^^^ In contrast , only one of the three bladder cell lines ( RT 4 ) had functional p 53 , and all of the bladder lines had readily detectable levels of Bcl 2 and low levels of Bax . ^^^ The differences in the ratio of Bax : Bcl 2 could contribute to the differential sensitivity of the two tumor types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A peptide sequence from Bax that converts Bcl 2 into an activator of apoptosis . ^^^ Bcl 2 and Bax are members of a family of cytoplasmic proteins that regulate apoptosis . ^^^ The two proteins have highly similar amino acid sequences but are functionally opposed : Bcl 2 acts to inhibit apoptosis , whereas Bax counteracts this effect . ^^^ The antagonism appears to depend upon dimerization between Bcl 2 and Bax , but its mechanism is otherwise unknown . ^^^ These findings imply that a specific region in Bax confers an active propensity for apoptosis in mammalian cells and support the view that Bcl 2 may block death primarily by suppressing Bax activity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of bcl 2 , bax , bcl 10 , and mcl 1 expression in prostate cancers . ^^^ Using antibodies specific for the Bcl 2 , Bax , Bcl 10 , and Mcl 1 proteins in combination with immunohistochemical methods , we examined for the first time the expression of these bcl 2 family genes in 64 cases of adenocarcinoma of the prostate , including 10 Gleason grade 2 to 4 tumors , 21 grade 5 to 7 tumors , 17 grade 8 to 10 tumors , 8 lymph node metastases , and 8 bone metastases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Taken together , these results suggest that expression of Bcl XL is increased in undifferentiated primary colorectal cancers , often with accompanying reciprocal decreases in the anti apoptotic proteins Bcl 2 and Mcl 1 and the pro apoptotic protein Bak , whereas Bax expression is relatively constant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 and Bax are members fo a family of cytoplasmic proteins that influence cell survival . ^^^ Whereas increased expression of Bcl 2 or Bcl 10 promotes cell survival following withdrawal of survival factors , increased expression of Bax is thought to suppress survival . ^^^ Surprisingly , overexpression of Bax rescued populations of sensory neurons deprived of nerve growth factor , as did overexpression of Bcl 2 and two Bcl 10 variants , Bcl XL and Bcl Xbeta . ^^^ Whereas Bcl 2 overexpression did not affect the survival response of neurons to neurotrophic factors , Bax overexpression partially inhibited the action of neurotrophic factors . ^^^ Co injection of Bcl 2 and Bax expression vectors promoted the survival of neurotrophic factor deprived neurons if either was in excess , but failed to rescue neurons if they injected at a 1 : 1 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Parental and multidrug resistant HL 60 leukemia cell lines were used to study coupling of expression of apoptotic / cytostatic ( bcl 2 , bax , bclxL , p21 / Waf1 , and c myc ) genes during differentiation . ^^^ The bcl 2 mRNA was rapidly downregulated while bax and bclxL gene expression was not altered in both differentiating HL 60 and HL60 / ADR cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mutant p 53 gene expression was detected in the majority of the cell lines and no relationship between p 53 gene expression and the expression of either bcl 2 , bcl 10 or bax was observed . ^^^ No changes in bcl 2 , bcl 10 and bax gene expression were observed in multidrug resistant cell lines compared with their drug sensitive counterparts . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of a cell death inducing gene , Bax , was investigated in 52 cases of Hodgkin ' s disease in parallel with Epstein Barr virus and was compared with the immunodetection of other apoptosis regulating proteins , Mcl 1 , Bcl 2 , and Bcl 10 . ^^^ Of 48 ( 89 % ) Bax positive tumors , 43 were found to express apoptosis inhibiting proteins such as Mcl 1 or Bcl 2 . ^^^ With the exception of 1 case , all Bax positive tumors also expressed either Bcl 2 , Bcl 10 , Mcl 1 , or combinations of these anti apoptotic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The process is associated with a downregulation of the apoptosis preventing gene , bcl 2 , while the expression of bax and p 53 , genes usually involved in promoting apoptosis , persists . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic protein Bax heterodimerizes with Bcl 2 and homodimerizes with Bax via a novel domain ( BH 3 ) distinct from BH 1 and BH 2 . ^^^ Both BH 1 and BH 2 in the Bcl 2 protein are required for its function as an inhibitor of cell death and for heterodimerization with the proapoptotic protein Bax . ^^^ In this report , we mapped the region in Bax required for heterodimerization with Bcl 2 and homodimerization with Bax , using yeast two hybrid and in vitro protein protein interaction assays . ^^^ Neither the BH 1 nor the BH 2 domain of Bax was required for binding to the wild type Bcl 2 and Bax proteins . ^^^ Bax / Bax homodimerization was not dependent on the inclusion of the NH 2 terminal 58 amino acids of the Bax protein in each dimerization partner , unlike Bcl 2 / Bcl 2 homodimers which involve head to tail interactions between the region of Bcl 2 where BH 1 and BH 2 resides , and an NH 2 terminal domain in Bcl 2 that contains another domain BH 4 which is conserved among antiapoptotic members of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Forty six cervical intraepithelial neoplasias ( CIN ) I / II , 75 CIN 3 , and 60 invasive squamous cell carcinomas ( ISCC ) were investigated by immunohistochemical staining for bcl 2 protein ( bcl 2 ) , bax protein ( Bax ) , estrogen receptor ( ER ) , and progesterone receptor ( PR ) . ^^^ In the Bax or HPV DNA positive groups , bcl 2 positive cases in CIN I / II were in the minority , whereas in CIN 3 they constituted the majority . ^^^ In ISCC , bcl 2 positive was significantly lower than negative cases , not being associated with Bax and HPV DNA . ^^^ These results indicate that bcl 2 may play an important role in a relatively early stage of cervical tumorigenesis , in association with Bax expression and HPV infection . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By crossing baxalpha mice with bcl 2 transgenics we show that the critical nature of the Baxalpha : Bcl 2 ratio holds in primary T cells and that it can be manipulated to elicit a strong response to previously resisted stimuli . p 53 has a role in the regulation of apoptosis in response to DNA damaging agents . p 53 directly activates transcription of the bax gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We report here that apoptosis induced by PMA , sphingosine , and N , N dimethylsphingosine ( DMS ) was accompanied by a concomitant decrease of bcl 2 expression in both RNA and protein levels in HL 60 cells , while expression of bcl XL and bax mRNA did not change , and neither sphingosine nor DMS induced differentiation of HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analyzing the expression patterns for Bcl 2 and Bax , two proteins known to modulate the apoptotic response , we found a downregulation of Bcl 2 and enhanced expression of Bax in 32Dcl3 cells . ^^^ In 32Dcl3 / v Abl cells , Bcl 2 expression remained constant while Bax was upregulated , whereas in 32Dcl3 cells expressing bcrabl , there was continuous expression of Bcl 2 at a level greater than observed in 5 abl transformed cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The bax protein regulates apoptosis in a cellular pathway that involves both bcl 2 and p 53 , two molecules associated with human glioma tumorigenesis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of prostate cancer cell lines expressing bcl 2 with taxol induces bcl 2 phosphorylation and programmed cell death , whereas treatment of bcl 2 negative prostate cancer cells with taxol does not induce apoptosis . bcl 2 phosphorylation seems to inhibit its binding to bax since less bax was observed in immunocomplex with bcl 2 in taxol treated cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent work also has revealed the existence of several bcl 2 related genes that also can inhibit ( e . g . , bcl 10 ( L ) and Mcl 1 ) or activate ( e . g . , bax , bcl 10 ( s ) , bag , and bad ) apoptosis in several systems . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This protective effect of Bcl 2 occurs in the absence of significant variations , in the stimulated livers , in the level of expression of other proteins also involved in resistance or sensitivity to apoptosis , namely Bcl 10 , Bax , Bad , Bak , and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human germinal center B cells express the apoptosis inducing genes Fas , c myc , P 53 , and Bax but not the survival gene bcl 2 . ^^^ To understand survival / death control of germinal center B cell , the expression of four apoptosis inducing genes , Fas , c myc , Bax , and P 53 , together with the survival gene bcl 2 , has been analyzed herein among purified tonsillar naive , GC , and memory B cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Drug induced apoptosis in B cell chronic lymphocytic leukemia : relationship between p 53 gene mutation and bcl 2 / bax proteins in drug resistance . ^^^ We investigated the relationship among chemosensitivity to drug induced apoptosis in vitro , the presence of p 53 gene mutations , and the expression of bcl 2 and bax proteins in B cells from B cell chronic lymphocytic leukemia ( B CLL ) patients . ^^^ Immunoblot analysis of bcl 2 and bax expression revealed a correlation between drug induced apoptosis and the ratio of endogenous levels of bcl 2 to bax proteins . ^^^ B CLL cells with none to low bcl 2 / bax ratios were drug sensitive as compared to cells with intermediate to high ratios that were drug resistant ( P = 0 . 015 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Association of bcl 2 , bax , bcl xL and interleukin 1 beta converting enzyme expression with initial response to chemotherapy in acute myeloid leukemia . ^^^ Bax , a partner protein of bcl 2 with extensive aminoacid homology , is a promoter of apoptosis . ^^^ Apparently the equilibrium of bcl 2 and bax hetero and homodimers is important for the susceptibility of cells for stimuli inducing apoptosis . ^^^ In this study we determined the role of bcl 2 to bax expression ratio , bcl xL and ICE expression level for predicting clinical response to chemotherapy in acute myelold leukemia ( AML ) . ^^^ There was a significant correlation between bcl 2 to bax expression ratio and clinical response ( P < 0 . 005 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression level of two regulatory intracellular proteins , bcl 2 and bax , involved in apoptosis showed a decrease in bcl 2 and an increase in bax with time . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Co expression of Bax , a negative regulator of Bcl 2 , reverts both the cell death protective and proliferation retarding activities of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the expression of Bax protein and mRNA , a member of the bcl 2 family that is an effector of apoptotic cell death , after global ischemia in the four vessel global ischemia model in the rat and compared these results with the expression of the antiapoptotic gene bcl 2 . ^^^ Bax mRNA and protein are both expressed in CA 1 before delayed death , whereas bcl 2 protein is not expressed . ^^^ Bcl 2 protein expression , but not that of Bax , is increased in CA 3 , a region that is ischemic but less susceptible to ischemic injury . ^^^ In the dentate gyrus , both Bax and bcl 2 proteins are expressed . ^^^ The selective expression of Bax in Ca 1 supports the hypothesis that Bax could contribute to delayed neuronal death in these vulnerable neurons by an independent mechanism or by forming heterodimers with gene family members other than bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The susceptibility of normal and cancer cells to induction of apoptosis is also regulated by the balance between apoptosis inducing genes such as the tumor suppressor wild type p 53 , and c myc and bax , and apoptosis suppressing genes such as the oncogene mutant p 53 , and bcl 2 and bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neither cytotoxicity nor cytostasis correlated with expression of p21Waf1 / Cip1 Thy 4 cells sustained accumulation of high levels of Bax in a wtp 53 independent and dThd independent manner and survival was associated with upregulated expression of Bcl 2 . ^^^ Data suggest that resistant cells ( Thy 4 ) can sustain high Bax expression and that Bcl 2 is upregulated in response to an apoptotic stimulus due to the absence of negative regulation by wtp53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The authors examined the expression of the apoptosis related modulators , Fas ( receptor ) , Fas ligand , Bax , Bcl 2 , Bcl XL , and interleukin 1 beta converting enzyme ( ICE ) in corneal cells as candidate mediators of this response and tested the effect of Fas receptor stimulating antibody on corneal stromal fibroblast cells in vitro . ^^^ METHODS : Reverse transcription polymerase chain reaction was used to detect FAS , FAS ligand , Bax , Bcl 2 , Bcl XL , and ICE mRNA expression in primary cultures of human corneal epithelial , stromal fibroblast , and endothelial cells . ^^^ RESULTS : FAS , Fas ligand , Bax , Bcl 2 , Bcl XL , and ICE mRNAs are expressed in all three major cell types of the cornea . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of apoptosis regulating proteins , Bcl 2 , Bax , Mcl 1 , and Bcl 10 , was evaluated by immunohistochemical methods in 39 cases of thyroid carcinomas . ^^^ Normal thyroid tissues showed a consistent expression of Bcl 2 and Mcl 1 whereas Bax and Bcl 10 proteins were essentially absent from most follicular thyroid cells . ^^^ However , in undifferentiated tumors , both Bax and Bcl 2 were weakly expressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical detection of Bax and Bcl 2 proteins in gerbil hippocampus following transient forebrain ischemia . ^^^ Time course expression of Bax and Bcl 2 proteins , identified as apoptosis regulating molecules , was assessed in gerbil hippocampus following transient forebrain ischemia . ^^^ Brain sections from animals sacrificed at 48 , 72 , 96 h and 7 days following 5 min ischemia were immunohistochemically evaluated using polyclonal antibodies specific for Bax and Bcl 2 proteins , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the apoptosis regulating genes Bcl 2 , Bcl 10 , Bax , Mcl 1 , and p 53 analyzed in 4 cases of human immunodeficiency virus ( HIV ) associated Hodgkin ' s disease , in 36 cases of HIV related non Hodgkin ' s lymphomas ( NHLs ) , and in 109 cases of non HIV related NHLs by using immunohistochemistry . ^^^ For the HIV related NHL samples , 36 , 66 , 88 , 100 , and 94 % of the cases were Bcl 2 , Bcl 10 , Bax , Mcl 1 , and p 53 were found to be expressed in 69 , 65 , 82 , 83 , and 42 % , respectively . ^^^ Of interest , all cases with a positive expression of Bax were also found to express either Mcl 1 and / or Bcl 2 , suggesting that Mcl 1 and Bcl 2 may counteract the pro apoptosis function of Bax in vivo by protein protein interaction within the tumor cell , as demonstrated previously in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resistance to apoptosis plays an important role in tumors that are refractory to chemotherapy and ionizing radiation ( IR ) . bax , which forms a heterodimer with bcl 2 and accelerates apoptosis , is not , or only weakly , expressed in most human breast cancer cells , and weak bax expression is considered to be related to the resistance of breast cancer cells to apoptosis . bax expression vector was introduced to human breast cancer MCF 7 cells , which exhibit weak expression of bax , to demonstrate its role of modulating radiation induced apoptosis . bax overexpression in MCF 7 cells by stable transfection does not affect viability by itself , but each stable transfectant was more sensitive to IR than the parental MCF 7 cells . ^^^ IR upregulated p 53 and p21WAF1 about 5 to 10 fold and downregulated bcl 2 and bcl XL by 80 90 % at 6 hr in both parent and bax stably transfected MCF 7 cells to the same degree . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The gamma IFN dependent protection was due neither to down regulation of p 53 , nor to the p 53 induced genes , p 21 ( WAF 1 ) and bax , nor to up regulation of bcl 2 or bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recently we have shown that the expression of bax alpha , a death promoting member of the bcl 2 family , is down regulated in breast cancer and have provided evidence that low bax expression might contribute to the pathogenesis of breast cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 and Bax was also explored in controls and Bcl 2 transfectants after GSNO addition . ^^^ Moreover , GSNO induced increases in the steady state levels of Bax protein in parental and Bcl 2 transfected cells . ^^^ We conclude therefore , that Bcl 2 acts downstream of p 53 , presumably nullifying the NO mediated increase in Bax protein in RAW 264 . 7 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results show that : ( 1 ) As2O3 triggers relatively specific NB 4 cell apoptosis at micromolar concentration , as proved by morphology , histogramic related nuclear DNA contents , and DNA gel eletrophoresis . ( 2 ) As2O3 does not influence bax , bcl 10 , c myc , and p 53 gene expression , but downregulates bcl 2 gene expression at both mRNA and protein levels . ( 3 ) As2O3 induces a significant modulation of the PML staining pattern in NB 4 cells and HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results indicate that radiation elicited apoptosis of fetal brain cells is associated with activation of the p 53 system , probable increases in AP 1 Fos / JunB heterodimers , and an increased ratio of Bax to Bcl 2 + Bcl xL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry to Bcl 2 , Bax , c Myc , c Fos , Fos related , c Jun , Jun B and Jun D was used to study the involvement of these factors in ionizing radiation induced apoptosis in the cerebellum of the developing rat . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cellular prion protein ( PrP ) binds to the C terminus of Bcl 2 but not Bax . ^^^ In addition , we analyzed heteromeric interactions of Bcl 2 with PrP and two Bcl 2 related proteins , Bax and A 1 . ^^^ Bax binding required almost the entire Bcl 2 molecule , while A 1 bound to the amino terminal region ( residues 1 82 ) . ^^^ Second , molecules such as Bax and A 1 may serve to cap chains of Bcl 2 homodimers by interacting with dimerization domains in the extramembrane region . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fas , Bcl 2 family ( Bcl 2 , Bcl 10 and Bax ) and ICE family ( ICE , Ich 1 ) were found to be involved in tumorigenesis of certain brain tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in human gastric cancer tissue ] . ^^^ Bax , a homologue of Bcl 2 , counters the death repressor activity of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The bcl 2 protein became nearly undetectable , and expression of bax protein increased slightly ( the bax : bcl 2 ratio was 14 fold higher than in untreated cells ) . ^^^ In summary , our data demonstrate that the combination of both KH 1060 and 9 cis RA irreversibly and synergistically inhibited clonal growth , induced differentiation and apoptosis of HL 60 cells concomitantly with a very marked decreased expression of bcl 2 , and increased the bax : bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To characterize postpartum renal development , to identify the cellular origin of the cysts , and to provide insight into the role that bcl 2 deficiency plays in the cystogenic process , we examined the morphology of kidneys from bcl 2 ( / ) mice and wild type littermates [ bcl 2 ( + / + ) ] from birth ( P 0 ) to postpartum day 28 ( P 28 ) , determined whether abnormalities of cellular proliferation and apoptosis accompany cyst development , and characterized expression of the bcl 2 related protein , bax . ^^^ Bax protein is expressed in the distal tubule in kidneys of bcl 2 ( + / + ) and bcl 2 ( / ) mice and in some , but not all cysts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Also , tat transfected cells expressed increased amounts of bax , a bcl 2 family protein known to induce apoptosis . ^^^ While these results support reports in the literature of an active role for tat in inducing cell death in HIV infected individuals , they point to a new mechanism involving Tat mediated modulation of bcl 2 and bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential regulation of p 53 , c Myc , Bcl 2 and Bax protein expression during apoptosis induced by widely divergent stimuli in human hepatoblastoma cells . ^^^ Curcumin treatment however did not alter the expression levels of Bcl 2 and Bax proteins . p 53 protein accumulated slowly and decreased abruptly after reaching the maximum . ^^^ While most of these agents affect the expression levels of p 53 and c Myc similarly , none of them altered the expression levels of the Bcl 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , like Bcl 2 and Bcl 10 , the Bcl w protein promotes cell survival , in contrast to other close homologues , Bax and Bak , which facilitate cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of decidualization on the expression of bax and bcl 2 in the rat uterine endometrium . ^^^ These data indicate that the balance between bax and bcl 2 expression is altered during stromal cell differentiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax in relation to Bcl 2 and other predictive parameters in breast cancer . ^^^ CONCLUSION : Expression of Bax was coupled with negative histopathological features , especially when expression of Bcl 2 was downregulated concomitantly . ^^^ It may appear that alterations of the differential Bax / Bcl 2 expression pattern take part in deregulation of proliferation and loss of differentiation , thus playing an important role in malignant progression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this report , we examined the levels of expression of proteins that can either prevent apoptosis ( i . e . , Bcl 2 or the long form of Bcl 10 , designated Bcl x 1 ) or promote apoptosis ( i . e . , Bax or the short form of Bcl 10 , designated Bcl xs ) in proliferating benign and malignant endothelial cells ( ECs ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When HCD 57 cells were cultured in the absence of Epo , Bcl 2 and Bcl XL but not Bax were downregulated , and the cells underwent apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Down regulation of apoptosis related bcl 2 but not bcl xL or bax proteins in multidrug resistant MCF 7 / Adr human breast cancer cells . ^^^ Recent studies have shown that high levels of the apoptosis related proteins bcl 2 and bcl xL increase , while over expression of bcl xs or bax decreases , resistance to drugs that induce apoptosis in some human cancer cells . ^^^ Despite the down regulation of bcl 2 in MCF 7 / Adr cells and equal levels of bcl 10 , and bax proteins in both cell lines , cytoplasmic DNA histone complexes induced by doxorubucin , taxol , vincristine and VP 16 indicate that MCF 7 / Adr cells are highly resistant to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis sensitivity in chronic lymphocytic leukemia is determined by endogenous endonuclease content and relative expression of BCL 2 and BAX . ^^^ Apoptosis resistance was associated with elevated BCL 2 and BAX expression . ^^^ Interestingly , incubation in vitro led to down regulation of BCL 2 expression in both apoptosis sensitive and apoptosis resistant cells , whereas parallel down regulation of BAX occurred only in the resistant samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered expression of bcl 2 and bax mRNA in amyotrophic lateral sclerosis spinal cord motor neurons . ^^^ To study the potential role of apoptosis in the degeneration of these motor neurons , in situ hybridization was used to measure the expression of two apoptotic cell death genes , bcl 2 and bax , in control and ALS lumbar spinal cord sections . ^^^ Surviving lamina 9 motor neurons in ALS spinal cord sections also expressed bcl 2 mRNA , but at levels that were significantly and selectively decreased ( 4 . 7 fold ) compared with controls . bax mRNA hybridization signal was detected in several cells throughout the gray matter in control and ALS lumbar spinal cord , but was significantly and selectively increased ( 2 . 8 fold ) in ALS motor neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a member of the Bcl 2 family of proteins , has been shown to promote apoptosis while other members of the family , including Bcl XL and Bcl 2 , inhibit cell death induced by a variety of stimuli . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Programmed myocyte cell death was accompanied by a decrease in the expression of bcl 2 and an increase in the expression of bax . ^^^ In conclusion , the mechanical load produced by myocardial infarction and ventricular failure may affect the regulation of bcl 2 and bax in the viable myocytes , triggering programmed cell death and the remodeling of the ventricular wall . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , both alphaE 7 # 1 and alphaE 7 # 2 showed constitutively elevated BAX and lowered BCL 2 levels , compared to either alphaE 6 # 1 or alphaE 6 # 2 . ^^^ However , elevated BAX and reduced BCL 2 per se were insufficient to trigger apoptosis , as apoptosis occurred only after exposure to gamma radiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proto oncogene bcl 2 and its family members , bcl 10 and bax are recognized as major regulators of cell death and survival . ^^^ Here we have studied the expression of bcl 2 , bcl xL and bax mRNA in rat cerebellar granule neurons cultured under conditions which influence neuron survival . ^^^ Insulin like growth factor 1 and brain derived neurotrophic factor supported the survival of these neurons , but affected neither the expression of bcl 2 , bcl xL nor bax mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was associated with increased expression of the antiapoptosis protein Bcl 2 and decreased expression of bax , a known activator of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bak are Bcl 2 family members which contain Bcl 2 homology regions 1 , 2 , and 3 ( BH 1 , BH 2 , and BH 3 ) , which interact with E1B 19K and Bcl 2 and promote apoptosis . ^^^ It also interacted with Bcl 2 but not with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pattern of expression of Bcl 2 , Bcl xL , Bcl xS and Bax genes , selected because of their interrelated role in the control of apoptosis , was analysed in a series of CD5+ B cell chronic lymphoid leukaemias . ^^^ According to the functional role of Bcl 2 , Bcl xL , Bcl xS and Bax , these data indicate that the pattern of Bcl 2 family genes expression in leukaemic CD5+ B cells is skewed toward prevention of apoptosis and may thus favour the relentless accumulation of CD5+ leukaemic B cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Co ordinated downregulation of bcl 2 and bax expression during granulocytic and macrophage like differentiation of the HL 60 promyelocytic leukaemia cell line . ^^^ The bcl 2 protein suppresses apoptosis and the bax protein opposes the cytoprotective effect of bcl 2 . ^^^ We show here that bax protein also declined with a time course similar to the downregulation of bcl 2 following treatment of HL 60 with phorbol myristate acetate ( PMA ) , dimethyl sulphoxide ( DMSO ) or retinoic acid ( RA ) . ^^^ Co ordinate downregulation of bcl 2 and bax proteins may fine tune the induction of apoptosis during cellular differentiation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Localization of bcl 2 , bax , and bcl 10 mRNAs in the developing inner ear of the mouse . ^^^ In situ hybridization was employed to study the expression of bcl 2 mRNA and its family members , bax and bcl 10 mRNAs , in the developing inner ear . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunostaining for the apoptosis regulating proteins bcl 2 , bax , c myc and p 53 failed to show their involvement in KF disease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 protein , an inhibitor of apoptosis , and Bax , an accelerator of apoptosis , in ventricular myocytes of human hearts with myocardial infarction . ^^^ The ratio of bcl 2 protein , an inhibitor of apoptosis , to Bax protein , an inducer of apoptosis , determines survival or death after an apoptotic stimulus . ^^^ We speculated that bcl 2 or Bax expression is induced by ischemia and that it may be related to myocyte death in human hearts . ^^^ METHODS AND RESULTS : We studied immunohistochemically 37 autopsied human hearts ( acute MI , n = 15 ; old MI , n = 12 ; normal hearts as a control , n = 10 ) with the use of bcl 2 and Bax antibodies . ^^^ CONCLUSIONS : bcl 2 protein is induced in salvaged myocytes at the acute stage of infarction , but Bax protein is overexpressed at the old stage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because expression of apoptosis associated molecules , such as c Myc , Bcl 2 , and Bax , was similar between U 937 and UK 110 cells an undetermined pathway for apoptosis through Fas and p 55 TNFR could be mutated in UK 110 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Northern and Western analysis revealed high levels of Bax and p 53 , and low or undetectable levels of Bcl 2 and Bcl xS under all treatment condition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the expression of Fas and bcl 2 family gene products , such as Bcl 2 , Bcl 10 , Bax , and Mcl 1 , in human tonsillar B cells . ^^^ The addition of anti Ig did not significantly change Bcl 2 , Bax , and Mcl 1 levels compared to those of B cells activated by CD40L alone . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered expression of Bcl 2 , Bcl 10 , Bax , and c Fos colocalizes with DNA fragmentation and ischemic cell damage following middle cerebral artery occlusion in rats . ^^^ A decrease in immunoreactivity for Bcl 2 , and Bcl 10 and an increase in immunostaining for Bax was observed exclusively in neurons within the ischemic cortex and thalamus . ^^^ By reverse transcription and polymerase chain reaction it was demonstrated that levels of bcl 2 mRNA markedly decreased in the ipsilateral hemisphere , whereas the amount of bax mRNA was elevated . ^^^ These findings suggest that a shift in the ratio of cell death repressor Bcl 2 to cell death effector Bax and a concomitant activation of c Fos may contribute to neuronal apoptosis in the infarcted thalamus and cortex . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This spontaneous cell death could be prevented by interleukin 2 ( IL 2 ) and was related to a decreased expression of Bcl 2 but not Bax or Bcl XL , additional molecules that promote or prevent apoptosis , respectively , as well as an increase in CD 95 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As compared to HL 60 / neo , HL 60 / Bcl 2 cells contained significantly higher ( approximately 10 fold ) p26Bcl 2 , but equivalent levels of Bax and undetectable levels of Bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present work we have analyzed Bcl 2 and Bax protein expressions in MCF 7 and T 47 D , 2 hormone dependent breast cancer cell lines , by immunoblots . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax has been shown to promote neuronal death by interacting with Bcl 2 family members while Gadd 45 expression has been associated with suppression of the cell cycle and DNA repair . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the cell death associated genes bcl 2 and bax in mammary epithelia , and found that although the expression of Bcl 2 did not correlate with cell survival , increased levels of Bax were associated with apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis regulating proteins Bcl 2 , Bax , Bcl 10 , Bak , and Mcl 1 were examined by immunohistochemical methods in 48 archival specimens of adenocarcinoma of the stomach , and the results were correlated with tumor histology ( intestinal versus diffuse pattern ) and clinical stage ( early versus late stage disease , ie , stages 1 and 2 versus stage 3 ) . ^^^ Tumor cells containing immunostaining for the anti apoptotic proteins Bcl 2 , Bcl 10 , and Mcl 1 were present in 26 ( 54 % ) , 41 ( 85 % ) , and 36 ( 75 % ) of the 48 cases evaluated , respectively , whereas immunopositivity for the pro apoptotic proteins Bax and Bak was found in 44 ( 92 % ) and 42 ( 88 % ) specimens Comparisons of these immunostaining results with tumor histology revealed statistically significant differences for Bax ( P = 0 . 03 ) , Bcl 10 ( P = 0 . 003 ) , and Mcl 1 ( P = 0 . 005 ) , which were all more frequently immunopositive for tumors with an intestinal than a diffuse histological pattern ( chi 2 analysis ) . ^^^ In contrast , the percentage of Bcl 2 immunopositive tumor cells was higher in tumors with diffuse histology compared with intestinal ( 32 + / 5 % versus 12 + / 5 % ; P = 0 . 01 ) , whereas the percentages of Bax and Bak immunopositive tumor cells were not significantly different between these two histological types . ^^^ The immunointensity for the Bcl 2 , Bcl 10 , and Mcl 1 proteins was stronger in tumor cells compared with normal foveolar cells in 7 ( 21 % ) , 15 ( 44 % ) , and 8 ( 2 . 1 % ) of 34 cases , respectively , whereas the immunointensity of the proapoptotic proteins Bax and Bak was reduced compared with normal cells in 8 ( 24 % ) and 24 ( 71 % ) cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Deletion of either BH 1 or BH 2 nullifies the ability of Bcl 2 to : ( a ) suppress death in mammalian cells : ( b ) block Bax induced lethality in yeast ; and ( c ) heterodimerize with Bax . ^^^ In contrast , deletion of the BH 4 domain of Bcl 2 nullifies anti apoptotic function and homodimerization , but does not impair binding to the pro apoptotic protein Bax . ^^^ Taken together , the data suggest the possibility that both Bcl 2 / Bcl 2 homodimerization and Bcl 2 / Bax heterodimerization are necessary but insufficient for the anti apoptotic function of the Bcl 2 protein . ^^^ In contrast , Bcl 2 / Bax heterodimerization involves a tail to tail interaction , that requires the portion of Bcl 2 where BH 1 , BH 2 , and BH 3 reside and a central region in Bax where the BH 3 domain is located . ^^^ Thus , Bcl 2 may suppress cell death at least in part by binding to Bax via the BH 3 domain and thereby preventing formation of Bax / Bax homodimers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Messenger RNA ( mRNA ) expression of bcl 2 , bcl 10 , bax , and c myc in T and B cells was determined by enzyme linked immunosorbent assay polymerase chain reaction ( ELISA PCR ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , we examined the effects of 4 HPR on components of apoptosis pathway ( i . e Bcl 2 and Bax ) and apoptotic death in both estrogen receptor positive and estrogen receptor negative cell lines . ^^^ We found that : ( 1 ) 4 HPR treatment resulted in decreased Bcl 2 mRNA but not Bax mRNA levels ; ( 2 ) the effect of 4 HPR on Bcl 2 mRNA level was different from other retinoids ; ( 3 ) 4 HPR treatment induced apoptosis in both estrogen receptor positive and negative cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using in situ hybridization , Northern blotting and RT PCR we studied the post ischemic expression of bcl 2 , bcl 10 , bax and ICE . ^^^ One day following 5 min or 10 min of global ischemia bcl 2 and bcl 10 mRNAs were induced in CA 1 hippocampal pyramidal neurons while bax was unchanged . ^^^ By 72 h after ischemia the expression of bcl 2 , bcl 10 and bax mRNAs decreased in CA 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Flt 3 ligand stimulates proliferation and inhibits apoptosis of acute myeloid leukemia cells : regulation of Bcl 2 and Bax . ^^^ We evaluated the effects of recombinant human flt 3 L on proliferation , apoptosis , and Bcl 2 and Bax expression in primary AML cells and compared them with those of stem cell factor ( SCF ) . ^^^ Cellular Bcl 2 and Bax levels were determined separately for apoptotic and nonapoptotic cells by flow cytometry . ^^^ Cells undergoing spontaneous apoptosis had low Bcl 2 and high Bax levels , whereas nonapoptotic cells had high Bcl 2 and low Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BCL 2 family of proteins consists of both antagonists ( e . g . , BCL 2 ) and agonists ( e . g . , BAX ) that regulate apoptosis and compete through dimerization . ^^^ The BH 1 and BH 2 domains of BCL 2 are required to heterodimerize with BAX and to repress cell death ; conversely , the BH 3 domain of BAX is required to heterodimerize with BCL 2 and to promote cell death . ^^^ To extend this pathway , we used interactive cloning to identify Bid , which encodes a novel death agonist that heterodimerizes with either agonists ( BAX ) or antagonists ( BCL 2 ) . ^^^ Mutagenesis revealed that an intact BH 3 domain of BID was required to bind the BH 1 domain of either BCL 2 or BAX . ^^^ In contrast , the only BID BH 3 mutant that retained death promoting activity interacted with BAX , but not BCL 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Amyloid beta peptide of Alzheimer ' s disease downregulates Bcl 2 and upregulates bax expression in human neurons . ^^^ We find that although little neuronal apoptosis is induced by either peptide after 3 d of treatment , Abeta ( 1 42 ) provokes a rapid and sustained downregulation of a key anti apoptotic protein , bcl 2 , whereas it increases levels of bax , a protein known to promote cell death . ^^^ The control , reverse peptide Abeta ( 40 1 ) , does not affect either bcl 2 or bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Rapid modulation of the apoptosis regulatory genes , bcl 2 and bax by prolactin in rat Nb 2 lymphoma cells . ^^^ The present study was conducted to determine whether PRL stimulation in lactogen dependent Nb 2 11 or autonomous Nb 2 SFJCD1 cultures alters expression of bcl 2 or bax , genes that suppress or facilitate apoptosis , respectively . ^^^ These results demonstrate that altered expression of bcl 2 and bax are each associated with PRL stimulated cell cycle progression in Nb 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For example , apoptotic death depends on the balance of Fas , Fas ligand , nuclear factors ( eg , bcl 2 , bax , and myc ) , cytokines , neuropeptides , and cell membrane interactions with extracellular matrix . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting revealed that BAX transfectants expressed a mean of 10 fold increased levels of BAX compared with neo transfected control clones , with similar levels of BCL 2 and BCL xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a member of the Bcl 2 family of proteins , has been shown to accelerate apoptosis induced by growth factor withdrawal , gamma irradiation , and the chemotherapeutic agent , etoposide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of clinical chemoresistance by bcl 2 and bax oncoproteins in B cell chronic lymphocytic leukaemia . ^^^ The bcl 2 oncoprotein is a regulator of apoptosis and the activity of this protein is opposed by bax , a homologous protein that accelerates the rate of cell death . ^^^ B lymphocyte bcl 2 and bax protein levels were found to be significantly altered in B CLL patients when compared to those of a normal control group . ^^^ Increased bcl 2 / bax ratios were observed in both the treated and untreated patients when compared to those of normal controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Failure of C 2 ceramide to induce apoptosis in Raji cells might be explained by the lack or low expression of apoptosis inducing proteins by two lines of evidence : ( 1 ) Raji cells were resistant to apoptosis induced by ceramide even in the presence of transcription / translation inhibitors ; ( 2 ) Bax protein expression was not detectable in Raji cells , although Bcl 2 protein expression in Raji cells was even less than that in HL 60 and U 937 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The role of BCL 2 and bax protein in monocyte mediated apoptosis in human leukemic cell lines . ^^^ In the present study , the relationship between apoptosis and the apoptosis related proteins , bcl 2 and bax , was investigated in a number of human leukemic cell lines . ^^^ The expression of bcl 2 and bax protein was determined in both groups of leukemic cell lines by flow cytometry ( bcl 2 and bax ) and Western blotting ( bcl 2 ) at baseline level and after incubation with monocyte supernatant after different time periods . ^^^ No clear relation was found between baseline bcl 2 or bax protein expression and the occurrence of apoptosis after incubation with monocyte supernatant . ^^^ Also , the ratio bax / bcl 2 decreased in HL60D3 , but especially in U 937 and HL 60 cells , increased slightly in THP 1 and KG 1 cells , and remained essentially unchanged in K 562 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the discovery of Bcl 2 a decade ago , several other cellular and viral genes encoding homologous proteins have been identified , some of which suppress cell death akin to Bcl 2 ( Bcl XL , Mcl 1 , A1 / Bfl 1 , Nr 13 , Ced 9 , BHRF 1 ) and others which promote apoptosis ( Bax , Bcl Xs , Bak , Bik , Bad ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All lines showed constitutive expression of bcl 2 and bcl XL ( the suppressors of apoptosis ) with low and non inducible levels of bax ( a promoter of apoptosis ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect is achieved in the absence of any detectable changes in the levels of BCL 2 , BAX or BCL 10 proteins and is independent of proliferative , MAP kinase dependent effects of BCR ABL kinase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Like BAX , another member of the BCL 2 family and a p 53 regulated gene , the induction of BCL 10 ( L ) was dependent on normal p 53 function and required that cells have an apoptosis susceptible phenotype . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related factors p 53 , Bcl 2 , and Bax in neuroendocrine lung tumors . ^^^ As p 53 mutations may favor escape from apoptosis through changes in Bcl 2 Bax expression balance , which are survival and apoptotic genes , respectively , we studied 121 NE lung tumors ( 16 typical carcinoids ( TC ) , 5 atypical carcinoids ( AC ) , 29 large cell NE carcinomas ( LCNECs ) , and 71 small cell lung carcinomas ( SCLCs ) using immunohistochemistry . ^^^ There was an inverse correlation ( P < 0 . 0001 ) between the scores of Bax and Bcl 2 expression in individual tumors and a significant inversion of the Bcl 2 . ^^^ Bax ratio between low grade ( typical and atypical carcinoids ) and high grade ( LCNECs and SCLCs ) tumors with a predominant Bax expression in the first group and predominant Bcl 2 expression in the second . ^^^ Whereas carcinoids had variable apoptotic indexes , LCNECs had high indexes ( 1 . 3 to 6 . 8 % ) , Bcl 2 overexpression , Bax down regulation , and Bcl2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression pattern of candidate cell death effector proteins Bax , Bcl 2 , Bcl 10 , and c Jun in sensory and motor neurons following sciatic nerve transection in the rat . ^^^ It has been demonstrated that protein levels of cell death repressor Bcl 2 and cell death promotor Bax determine the threshold for neuronal cell death and that their expression is dynamically modulated at the onset of neurodegeneration . ^^^ In the L 5 dorsal root ganglia ( DRG ) levels of Bax , Bcl 2 , and Bcl 10 proteins were highly variable . ^^^ These findings indicate that the high susceptibility of central neurons and small sized DRG neurons to axotomy induced cell death might be related to their low ratio of cell death repressor Bcl 2 and Bcl XL to cell death promotor Bax expression . ^^^ It should be noted , however , that numerous strongly Jun positive DRG neurons contained low levels of Bax or high levels of Bcl 2 and Bcl 10 immunoreactivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , both cell types expressed equivalent levels of Bcl xL , Bax , Bcl 2 , Myc , retinoblastoma , p21cbor abl , and p145abl proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Modulation of apoptosis associated genes bcl 2 , bcl 10 , and bax during rat liver regeneration . ^^^ Western blot analyses of Bcl 2 and Bcl 10 proteins showed no significant change through 96 h of LR , whereas Bax protein levels cycled in parallel with its mRNA . ^^^ Interestingly , novel Bax and Bcl 2 cross reactive proteins of 31 and 32 kDa , respectively , were detected in nuclei isolated from quiescent liver . ^^^ In conclusion , the apoptosis associated genes bcl 2 , bcl 10 and bax are modulated at the transcript and protein levels during LR , suggesting a role for these gene products in normal liver growth . ^^^ Furthermore , unlike bax , steady state protein and transcript levels are uncoupled for both bcl 2 and bcl 10 , suggesting a role for translational regulation during LR after PH . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis resistance in the metastatic cells was associated with higher levels of expression of the cell death suppressor BCL 2 and lower levels of the death promoters BAX and BAK than were detected in the nonmetastatic LNCaP Pro 5 cells , whereas levels of two other BCL 2 family members ( BCL 10 ( L ) and BAD ) were indistinguishable . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Localization of Bax and Bcl 2 proteins , regulators of programmed cell death , in the human central nervous system . ^^^ Bax and Bcl 2 proteins are identified as regulating molecules for programmed cell death . ^^^ In this study , localization of Bax and Bcl 2 proteins was investigated in the human central nervous system using autopsy cases without any neurological disorder . ^^^ Most neurons in human cerebral cortex , basal ganglia and brain stem were positive for both Bax and Bcl 2 proteins , whereas Purkinje cells in cerebellum and neurons in hippocampal CA 1 , CA 2 and CA 3 regions were positive for Bax but negative or weakly positive for Bcl 2 . ^^^ A possible relationship between the localization of Bax or Bcl 2 proteins and the cell vulnerability in central nervous system is discussed . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the nervous system , genes have been identified which either 1 ) promote apoptosis : Bax , Bcl xS , c fos , c jun , p75NGFR and ICE like proteases , or 2 ) block apoptosis : Bcl 2 and Bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect of IFN alpha 2 was neither explained by a down regulation of the Apo 1 / Fas receptor nor caused by modulation of the expression levels of c myc , bcl 2 , bcl xL , bax or p 53 genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The adenovirus E1B 19K product is homologous in sequence and in function to Bcl 2 , both of which bind to and inhibit the activity of Bax and thereby suppress apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Previous in vitro studies have shown that the presence of high levels of Bax protein accelerated the rate of cell death following growth factor deprivation and that the ratio of cell death repressor Bcl 2 to cell death effector Bax may determine the susceptibility to apoptosis . ^^^ Both Bcl 2 and Bax protein expression has been detected in sympathetic neurons in vivo , and overexpression of bcl 2 in cultured sympathetic neurons prevented apoptosis after deprivation of nerve growth factor ( NGF ) . ^^^ In the present study , we investigated the expression of bax and bcl 2 in primary cultures of sympathetic neurons from rat superior cervical ganglia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The onco proteins Bcl 2 and Bax and the scavenger proteins Cu / Zn superoxide dismutase ( SOD 1 ) and Mn superoxide dismutase ( SOD 2 ) are among the 40 50 proteins whose synthesis is altered by ( ) deprenyl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax protein expression is increased in Alzheimer ' s brain : correlations with DNA damage , Bcl 2 expression , and brain pathology . ^^^ Most Bax positive neurons showed either TdT labeled nuclei or Bcl 2 immunoreactivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Vitamin C and E prevent lipopolysaccharide induced apoptosis in human endothelial cells by modulation of Bcl 2 and Bax . ^^^ Furthermore , lipopolysaccharide treatment upregulated Bax , which heterodimerizes with and thereby inhibits Bcl 2 . ^^^ The reduction of lipopolysaccharide induced apoptosis by vitamin C and E was paralleled by an increase in Bcl 2 and a decrease in Bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It has been shown previously that wild type p 53 activity can simultaneously up regulate Bax , a protein which predisposes cells to programmed cell death ( PCD ) , and down regulate Bcl 2 , a protein which antagonizes PCD . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the present studies were conducted to determine whether levels of mRNA encoding BCL 2 and related members of this gene family ( BAX and BCL Xshort , which induce apoptosis ; BCL Xlong , a BCL 2 homologue that prevents apoptosis ) differed in functional ( Day 21 of pregnancy ) versus regressed ( Day 21 of the estrous cycle ) CL in the bovine ovary . ^^^ Levels of mRNAs encoding p 53 , a transcriptional regulator of the bcl 2 and bax genes , and interleukin 1beta converting enzyme ( ICE ) , a protein recently implicated in the induction of apoptosis whose expression may be enhanced by oxidative stress , were also assessed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , Mcl 1 was found to interact with Bax , a member of the Bcl 2 family that promotes cell death as a homodimer but that can heterodimerize with Bcl 2 to promote cell viability . ^^^ Taken as a whole , these findings provide further parallels between Mcl 1 and Bcl 2 , showing that Mcl 1 can interact with Bax in hematopoietic FDC P 1 cells and can prolong cell viability under a variety of cytotoxic conditions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax alpha can heterodimerize with Bcl 2 and Bcl 10 ( L ) , countering their effects , as well as promoting apoptosis on overexpression . ^^^ Further to this , mature T cells overexpressing Bax alpha have lower levels of p27Kip1 and enter S phase more rapidly in response to interleukin 2 stimulation than do control T cells , while the converse is true of bcl 2 transgenic T cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry showed that the bcl 2 protein decreased from nearly 100 % of the wild type NB 4 cells to 2 % after incubation with a combination of KH 1060 and 9 cis RA , and the bax protein increased from 50 % of wild type NB 4 cells to 92 % after culture with both analogs ( 5 10 10 ( 7 ) M , 3 d ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of P glycoprotein ( PGP ) , MRP or LRP has been characterized as the ' proximal ' , while overexpression of the anti apoptosis Bcl 2 or Bcl xL relative to the pro apoptosis Bax protein has been recognized as the ' distal ' mechanism of multidrug resistance in human AML cells . ^^^ For this , immunoblot analyses were performed to determine the expression of PGP , MRP , Myc , Bcl 2 , Bcl xL and Bax on either the multidrug resistant HL 60 sublines created under the selection pressure of doxorubicin ( HL 60 / AR ) , paclitaxel ( HL 60 / TAX1000 ) or vincristine ( HL 60 / VCR ) , or sublines created by transfection and overexpression of the bcl 2 ( HL 60 / Bcl 2 ) or bcl xL gene ( HL 60 / Bcl xL ) . ^^^ In addition , these multidrug resistant cells possess 1 . 5 to 2 . 5 fold higher Bcl 2 , while their Bax and Myc levels are similar to those in the control HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of oncogenes regulating apoptotic cell death revealed a marked decrease of bcl 2 in combination with a moderate reduction of bax protein , but a striking overexpression of the long form of the bcl 10 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The process o apoptosis in keratinocytes was dissected at the molecular level and found to be correlated with increased expression of Bax and decreased levels of Bcl XL , with no role for either Bcl 2 or Bcl XS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical examination of the expression of the apoptotic checkpoint proteins , Bcl 10 , Bax and Bcl 2 , demonstrated that they are expressed in the terminal endbud . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Bcl 2 homolog encoded by Kaposi sarcoma associated virus , human herpesvirus 8 , inhibits apoptosis but does not heterodimerize with Bax or Bak . ^^^ Interestingly , KS bcl 2 neither homodimerizes nor heterodimerizes with other Bcl 2 family members , suggesting that KSbcl 2 may have evolved to escape any negative regulatory effects of the cellular Bax and Bak proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the ratio of Bcl 2 to Bax was decreased by the enhanced expression of Bax immediately after FTY 720 treatment , resulting in rapid cell death acceleration . ^^^ The surviving cells ( FTY 720 resistant cells ) at 2 and 3 hr after culture showed a similar ratio of Bcl 2 to Bax as was observed in the control cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the effect of receptor selective retinoid treatment on the expression of Bcl 2 and Bax oncogenes by reverse transcription polymerase chain reaction ( RT PCR ) and immunoblot analysis in RARs and RXR alpha transfected HL 60 cells . ^^^ Our results show that activation of RXR alpha results in apoptosis via down modulation of Bcl 2 mRNA as well as its gene product expression with no change in Bax mRNA expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic DNA ladders could be detected when cancer cells were treated with GL 331 for 24 h even if the Bcl 2 and Bax protein levels were not altered during the period . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analyses were performed with specific antibody against BCL 2 , BCL XS / L , BAX , JUNB , c JUN , ICH 1L , c FOS , RB , CDK 2 , and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis , cell proliferation and expression of Bcl 2 and Bax in gastric carcinomas : immunohistochemical and clinicopathological study . ^^^ To clarify the relation between bcl 2 and bax protein ( Bcl 2 and Bax ) expression with regard to apoptosis and cell proliferation , 82 gastric carcinomas were immunohistochemically investigated . ^^^ The AIs in the high Bcl 2 immunoreactive score group were significantly smaller than in either the low or the negative categories , whereas they were relatively elevated in the high Bax score group . ^^^ In addition , an inverse correlation between Bcl 2 and Bax expression was revealed for both AIs and MIs . ^^^ Although depth of tumour invasion and lymph node status were clearly associated with favourable outcome , no relation between survival rates and average values of either AIs or MIs , or immunoreactive scores for Bcl 2 and Bax was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We report here on the high detection rate of the Bcl 10 protein found in 86 % of Hodgkin ' s disease samples and on the significance regarding its complex role among the Bcl 2 family of proteins : Bcl 10 is known to heterodimerize with Bcl 2 ( an anti apoptosis protein ) and with Bax , a potent inducer of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Synovial T cells in rheumatoid arthritis are highly differentiated and express a phenotype suggesting susceptibility to apoptosis ( CD45RB dull , CD45RO bright , Bcl 2 low , Bax high , Fas high ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alterations in genes such as p 53 , mdm 2 , bcl 2 and bax which control entry into apoptosis may cause drug resistance . ^^^ In addition , although Bax and Bcl 2 are important regulators of apoptosis and the levels of these proteins are elevated in CLL cells compared with normal B cells , the levels of Bax and Bcl 2 , or the Bax : Bcl 2 ratio , are not important determinants of drug sensitivity in this leukemia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A sufficient amount of expression of apoptosis protecting factors such as bcl 2 is needed to neutralize high bax levels , and a lack of this expression will secondarily promote muscle fiber death in defective innervation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical localization of Bcl 2 and Bax proteins in in situ and invasive duct breast carcinomas . ^^^ Bcl 2 and Bax proteins are coded by a family of genes that take part in the manteinance of the balance between cell proliferation rate and programmed cell death in multicellular organisms . ^^^ The Bax gene acts as promoter of cell death by opposing the death protector effect of the Bcl 2 gene . ^^^ Expression of the Bcl 2 and Bax proteins has been investigated in 58 cases of duct carcinoma in situ ( DCIS ) and duct invasive and invasive lobular carcinomas ( IC ) of the breast . ^^^ Therefore , it appears that Bax protein expression , especially in DCIS , relates to more aggressive neoplasms while Bcl 2 protein expression is associated with less aggressive malignant lesions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of bcl 2 and bax in squamous cell carcinomas of the oral cavity . ^^^ Recent evidence suggests that the bcl 2 protein is regulated by a homologous protein bax which counteracts its effects and promotes apoptosis . ^^^ We studied bcl 2 and bax protein expression in adjacent serial sections of 30 squamous cell carcinomas of the oral cavity and correlated this with tumour differentiation . ^^^ Overall , moderate or marked immunostaining for bcl 2 was identified in 18 / 30 ( 60 % ) carcinomas and for bax in 19 / 30 ( 63 % ) tumours . ^^^ In the well differentiated tumour islands , there was inverse topographic distribution of bcl 2 and bax , with both proteins showing a pattern that recapitulated normal epithelium . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis is accompanied by changes in Bcl 2 and Bax expression , induced by loss of attachment , and inhibited by specific extracellular matrix proteins in mammary epithelial cells . ^^^ These data suggest that Bcl 2 and Bax might be intracellular mediators of signals that influence MEC apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the apoptosis associated proteins Bcl 2 and Bax was quantitated by flow cytometry ( FCM ) in chemosensitive testicular germ cell tumor NT 2 cells , and the results were compared with those obtained by Western blotting . ^^^ NT 2 cells were incubated with cisplatin ( 3 . 1 microM for 2 h at 37 degrees C ) , and 24 , 48 , and 72 h later were analyzed for induction of apoptosis , and for modulation of the expression of cell death suppressing protein Bcl 2 , as well as cell death promoting protein Bax . ^^^ Subsequently , we determined the expression of the Bcl 2 and Bax proteins by FCM and Western blotting before and after drug treatment . ^^^ NT 2 cells had low constitutive expression levels of Bcl 2 and elevated constitutive expression levels of Bax protein , as determined by both methods . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunolocalization of the apoptosis regulating proteins Bcl 2 and Bax in human endometrium and isolated peritoneal fluid macrophages in endometriosis . ^^^ Apoptosis , which occurs in both endometrium and peritoneal fluid macrophages , is controlled in part by members of the Bcl 2 / Bax family of proteins . ^^^ Here , through immunohistochemical staining , we investigated the Bcl 2 / Bax status in endometrium and peritoneal fluid macrophages in endometriosis . ^^^ Bcl 2 / Bax immunoreactivity was found predominantly in the glandular epithelial cells , mainly during the proliferative phase of the menstrual cycle for Bcl 2 but throughout the entire menstrual cycle for Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to determine whether the process of programmed cell death is distinct between cord blood and peripheral blood lymphocytes , we analyzed the expression of fas and bax ( apoptosis promoting genes ) and bcl 2 and bcl xL ( apoptosis inhibiting genes ) at protein or mRNA levels using flow cytometry and quantitative PCR methods , respectively . ^^^ We observed that cord blood T cell subsets expressed lower levels of Fas and Bcl 2 , a low bcl 2 / bax ratio , and higher bcl xL compared to peripheral blood . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas the protein product of the Bcl 2 gene inhibits apoptosis , the protein product of the Bax gene acts as a promoter of apoptosis . ^^^ To gain insight into the regulation of apoptosis in vascular smooth muscle cells in arterial hypertension , we investigated the expression of the proteins Bcl 2 and Bax in small intramyocardial arteries of 36 week old normotensive Wistar Kyoto rats ( WKY ) and spontaneously hypertensive rats ( SHR ) . ^^^ Compared with WKY , untreated SHR exhibited increased ( P < . 001 ) Bcl 2 expression and similar Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Biochemical analysis revealed that all three proteins can associate with Bax and Bak , members of the Bcl 2 protein subfamily that can facilitate apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no changes in Bcl 2 or Bax protein levels that could account for the apoptosis inducing effects of IFN alpha in these cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mercaptopurine decreases the Bcl 2 / Bax ratio and induces apoptosis in activated splenic B lymphocytes . 6 Mercaptopurine and related purine antimetabolites are used in the treatment of several B cell disorders . ^^^ The ratio between the amounts of Bcl 2 and Bax , two proteins involved in the control of apoptosis in mature B cells , markedly decreased as result of mercaptopurine treatment . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of Bcl 2 , Bcl 10 , Mcl 1 , and Bax in tumors of central and peripheral nervous system origin . ^^^ The expression of Bcl 2 , Bcl 10 , Mcl 1 , and Bax was examined by immunohistochemical methods in 93 tumors of nervous system origin , including 49 gliomas ( 30 astrocytomas and 19 glioblastoma multiforme ( GMs ) ) , 16 medulloblastomas ( MBs ) , 19 neuroblastomas ( NBs ; 9 undifferentiated and 10 differentiated ) , and 9 miscellaneous neuroectodermal neoplasms . ^^^ Among the 49 gliomas , immunopositivity ( defined as > or = 10 % ) was observed for Bcl 2 in 45 ( 92 % ) , Bcl 10 in 48 ( 98 % ) , Mcl 1 in 49 ( 100 % ) , and Bax in 48 ( 98 % ) of 49 specimens . ^^^ Of the 16 MBs , immunopositivity was found for Bcl 2 in 4 ( 25 % ) , Bcl 10 in 9 ( 56 % ) , Mcl 1 in 8 ( 50 % ) , and Bax in 16 ( 100 % ) of the cases . ^^^ The intensity of immunostaining was strong for Bcl 2 in only 1 ( 6 % ) specimen , for Bcl 10 in 3 ( 19 % ) , and for Mcl 1 in 2 ( 12 . 5 % ) , in contrast to Bax immunostaining , which was strong in 12 ( 75 % ) tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the level of two other p 53 regulated proteins , Bcl 2 and Bax , were examined . ^^^ These data strongly suggest that factors other than p 53 exert the major influence over Bcl 2 and Bax expression , although levels of the 90K MDM 2 protein are more likely to be regulated by p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present studies , we demonstrate that in human AML HL 60 cells that express Bcl 2 but little Bcl xL ( HL 60 / neo cells ) , paclitaxel induced phosphorylation of Bcl 2 is followed by increased intracellular free Bax levels . ^^^ Immunoprecipitation studies with anti Bcl 2 and / or anti Bcl 10 antibodies demonstrated that HL 60 / Bcl xL cells possess lower free Bax but higher levels of Bax heterodimerized to Bcl 2 and Bcl xL . ^^^ Following treatment of HL 60 / Bcl xL cells with paclitaxel , although Bcl 2 phosphorylation was observed , it was not followed by increased free Bax levels , cleavage of CPP32beta / Yama and poly ( ADP ribose ) polymerase , or induction of the DNA fragmentation of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This includes an increase in p 53 ( wild type ) , a decrease in BCL 2 , a p 53 dependent increase in the BCL 2 family member BAX , and increases in Growth Arrest and DNA Damage inducible ( GADD ) genes such as GADD 45 ; these changes occur as early events in a sequence that culminates in DNA damage induced apoptosis . ^^^ These findings demonstrate that cytotoxic DNA damage causes an increase in the expression of MCL 1 along with increases in GADD 45 and BAX and a decrease in BCL 2 . ^^^ Furthermore , while the increase in GADD 45 is seen both in cells that undergo growth arrest and in cells that undergo apoptosis in response to DNA damage , alterations in the profile of expression of BCL 2 family members occur exclusively in cells that undergo the apoptotic response , with some family members increasing through p 53 dependent ( BAX ) and others through p 53 independent ( MCL 1 ) pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression in thyroid tumours . ^^^ Bcl 2 and Bax proteins , which are involved in repressing and promoting programmed cell death , respectively , have been investigated immunohistochemically and by Western blot analysis in a series of thyroid tumours . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlative studies in vivo revealed decreased bcl 2 and increased bax gene expression in the renal cortex of diabetic db / db mice , and this was associated with increased apoptotic index in the tubular epithelium as compared with nondiabetic littermates . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , the molecular basis of some of these diseases ( eg , the overexpression of the Prad1 / CCND1 gene in mantle cell lymphomas , the relationship between bcl 2 and bax expression in chronic lymphocytic leukemia homeostasis , the role of p 53 tumor suppressor gene mutations in chronic lymphocytic leukemia progression ) are increasingly well known . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this review the incidence of apoptosis in the stem cells of both the small and large intestine will be discussed in relation to the expression of a number of apoptosis regulating genes ( e . g . p 53 , Bcl 2 , bax ) within these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BH 1 and BH 2 homology domains are highly conserved in ORF 16 , and ORF 16 heterodimerizes with Bcl 2 family members Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent studies indicate that the proto oncogene Bax , and other related proteins ( eg Bcl 2 ) may play a major role in determining whether cells will undergo apoptosis under conditions which promote cell death . ^^^ Increased expression of Bax has been found to promote apoptosis , while over expression of Bcl 2 can inhibit apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of two proto oncogenes that influence apoptosis , BCL 2 and BAX , was also determined . ^^^ The percentage of myocytes labeled with BCL 2 ( which protects cells against apoptosis ) was 1 . 8 times as high in the hearts of patients with cardiac failure as in the normal hearts , whereas labeling with BAX ( which promotes apoptosis ) remained constant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The in situ expression of Bcl 2 , Rb , p 21 , p 53 and Bax proteins , as well as the proliferation marker proliferating cell nuclear antigen ( PCNA ) were examined immunocytochemically in a selection of 38 stage and outcome identified NB tumours . ^^^ Although the tumour cell density of Bcl 2 , p 53 , Bax , PCNA and TUNEL positivity correlated with patient survival , a spatially organized expression pattern was further recognized in stroma poor differentiating tumours . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neonatal diethylstilbestrol treatment alters the estrogen regulated expression of both cell proliferation and apoptosis related proto oncogenes ( c jun , c fos , c myc , bax , bcl 2 , and bcl 10 ) in the hamster uterus . ^^^ As part of a project to investigate the molecular and cellular mechanisms responsible for this phenomenon , expression of several proto oncogenes ( c jun , c fos , c myc , bax , bcl 2 and bcl 10 ) was compared in estrogen stimulated uteri from control versus neonatally DES treated hamsters . ^^^ In conclusion , neonatal DES treatment induced persistent and epithelial cell specific imbalances in the estrogen regulated uterine expression of c jun , c fos , c myc , bax , bcl 2 , and bcl 10 proto oncogenes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ET 18 OCH 3 did not affect the expression of bcl 2 , bcl xL , or bax in HEL and HL 60 human leukemic cells but induced expression of c myc , an important effector of apoptosis in several systems . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 and Bcl 10 proteins suppress programmed cell death , whereas Bax promotes apoptosis . ^^^ We investigated the pattern of expression of Bcl 2 , Bax and Bcl 10 during neuronal differentiation and development . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Experiments using transgenic and gene knockout mice have revealed much about the roles played by Bcl 2 , Bcl 10 and Bax in regulating apoptosis in the immune system . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 ( L ) , and Bax are members of the Bcl 2 family that play key roles in the regulation of apoptosis . ^^^ Herein we report that in murine thymocytes , Bcl 2 is exclusively membrane bound , whereas Bax is present predominantly in the cytosol and Bcl 10 ( L ) is present in both soluble and membrane bound forms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 6 ligation modulates the Bcl 2 / Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti IgM . ^^^ However , anti CD 6 stimulation was found to protect against anti IgM induced apoptosis in B CLL . bax ( alpha ) upregulation and bcl 2 downregulation were found in anti IgM and glucocorticoid ( GCC ) induced apoptotic cells , respectively . ^^^ Furthermore , CD 6 cross linking downregulated bax ( alpha ) mRNA levels in anti IgM treated cells , resulting in an increased bcl 2 / bax ( alpha ) ratio . ^^^ These data suggest that an interaction between CD 6 and its ligand might contribute to B CLL survival through the modulation of the Bcl 2 / Bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression and regulation of Bcl 2 , Bcl xl , and Bax correlate with p 53 status and sensitivity to apoptosis in childhood acute lymphoblastic leukemia . ^^^ Bcl 2 and its homologue , Bcl x 1 , encode membrane associated proteins that protect neoplastic cells from DNA damage induced apoptosis , whereas Bax is a Bcl 2 antagonist that promotes cell death . ^^^ Treatment with IR ( 10 Gy ) induced both downregulation of Bcl 2 and upregulation of Bax at 2 to 5 hours post IR in 5 of 8 ( 63 % ) wt p53+ lines , leading to apoptosis . ^^^ Conversely , lines that failed to both downregulate Bcl 2 and upregulate Bax after IR were resistant to apoptosis . ^^^ These results suggest that expression of Bcl 2 but not Bcl x 1 is p 53 dependent and that IR induced downregulation of Bcl 2 and upregulation of Bax occur in most wt p53+ lines and are associated with radiosensitivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also show that Bax , a pro apoptotic member of the Bcl 2 family , interferes with interactions between calcineurin and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the loss of function S70A mutant can heterodimerize with its partner protein and death effector Bax , these findings demonstrate that Bcl 2 : Bax heterodimerization is not sufficient and Bcl 2 phosphorylation is required for full Bcl 2 death suppressor signaling activity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As the low level of bcl 2 and bax mRNA was not influenced by progesterone treatment , the observed changes in total amount of bcl 10 transcripts and spliced isoforms could represent the mechanism by which progesterone controls cell death in epithelial cells of the endometrium . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ten erythema multiforme and five control oral mucosa biopsy specimens were evaluated in immunohistochemically stained sections for apoptosis regulating proteins Bcl 2 , Bcl 10 , Bax , p 53 , Fas , and Fas ligand . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and its homologues , bcl 10 and bax , also increase in amount with bcl 2 and bcl 10 increasing more rapidly than bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inactivation of p 53 dependent apoptosis promotes oncogenic transformation , tumor development , and resistance to many cytotoxic anticancer agents . p 53 can transcriptionally activate bax , a bcl 2 family member that promotes apoptosis . ^^^ These p 53 controlled factors act synergistically with Bax to promote a full apoptotic response , and their action is suppressed by the Bcl 2 and E1B 19K oncoproteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In LMP 1 expressing clones , although the levels of Bcl 2 and Bax were similar to those in the clones of vector transfectants or parental cells , c Myc expression was significantly depressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To explore the mechanism of failure of the wild type p 53 in inducing apoptosis in thyroid cells , we investigated the expression of apoptosis related genes , bax , bcl 2 and fas / APO 1 following irradiation or induction of temperature sensitive p 53 . ^^^ The expression of Bax , Bcl 2 and Fas / APO 1 in human primary cultured thyroid cells did not change after irradiation . ^^^ Although enhanced expression of the bax mRNA level was observed , the expression of Bax , Bcl 2 and Fas / APO 1 protein did not change by shifting tsFRO cells to permissive temperature as well as irradiated primary cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The product of harakiri , Hrk , physically interacts with the death repressor proteins Bcl 2 and Bcl 10 ( L ) , but not with death promoting homologs , Bax or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we examined the sphingosine induced apoptosis of the androgen independent human prostatic carcinoma cell line DU 145 , which expresses bcl 10 ( L ) and Bax but not bcl 2 , and found that treatment of DU 145 cells with sphingosine suppressed bcl 10 ( L ) in both mRNA and protein levels but did not change bax expression at all . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , western blot analysis showed that the bcl 2 and bax proteins were constitutively elevated in ras transformed cells but not in parental NIH / 3T3 cells . ^^^ Thus , our data suggest that an activated ras oncogene can suppress alkylating agent induced apoptotic cell death by means of a defect in the signal transduction pathway regulating p 53 function and alteration in the expression of apoptotic ( bax ) or anti apoptotic proteins ( bcl 2 and hsp 70 ) . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Members of the Bcl 2 family ( including Bcl 2 , Bcl XL , and Bax ) play key roles in the regulation of apoptosis . ^^^ We have found that whereas Bcl 2 is predominantly membrane associated as previously reported , significant amounts of Bcl XL and most of the Bax proteins are not membrane associated and thus appear in the cytosolic fraction of thymocyte and splenocyte extracts . ^^^ For this analysis , we have produced monoclonal antibodies that are specific for known epitopes of Bax , Bcl 2 , and Bcl XL . ^^^ In the presence of nonionic detergent , the 6A7 antibody avidly binds the monomeric form of Bax , but not Bax complexed with either Bcl XL or Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Denervated muscle fibres revealed strong immunoreactivity of the anti apoptotic proteins bcl 2 and bcl xL , and the pro apoptotic factor bax . ^^^ In reinnervated muscle fibres , only bcl 2 was constantly upregulated while bcl xL and bax diminished after the 7th week . ^^^ Furthermore , increasing levels of bcl 2 are able to neutralize high apoptosis promoting bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IGF induced protection against dexamethasone was not associated with any alteration in quantitative or qualitative expression of BCL 2 , BAX or BCL 10 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The critical role played by the intracellular balance , probably hormonally controlled , of the BclxL and Bax proteins ( Bcl 2 is apparently not expressed in normal mouse testis ) in this early apoptotic wave is shown by the occurrence of a comparable testicular syndrome in mice defective in the bax gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 , Bax and p 53 apoptosis associated proteins were evaluated in immunohistochemically stained tissue sections according to staining intensity and pattern . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In all LBC tested , radiation induced apoptosis occurs in all phases of the cell cycle and appears not to directly involve changes in the levels of the apoptosis associated proteins bcl 2 , bax and mcl 1 . ^^^ Radiation induced cell death is not cell cycle phase specific , does not involve the regulation of bcl 2 , bax or mcl 1 , but is associated with changes in the phosphorylation state and activation of p34CDC2 kinase . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas the expression patterns of known cell death related genes ( bcl 2 and bax ) were not informative with respect to the cellular response patterns upon exposure to selenium compounds , time dependent and selenium species specific induction patterns were observed for gadd 34 , gadd 45 and gadd 153 genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in regenerating rat renal tubules following ischemic injury . ^^^ To define potential roles for bcl 2 and bax in adult kidney as regulators of regeneration , their expressions were characterized postischemic injury . ^^^ We conclude that the expressions of bcl 2 and bax in kidney are enhanced in a predictable pattern following acute ischemic injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of dehydroepiandrosterone and calorie restriction on the Bcl 2 / Bax mediated apoptotic pathway in p 53 deficient mice . ^^^ Modulation of apoptosis through altered expression of Bcl 2 and / or Bax may be a mechanism by which dehydroepiandrosterone ( DHEA ) administration and calorie restriction ( CR ) exert their chemopreventive effects in p 53 deficient ( p 53 / ) mice . ^^^ To further understand the molecular mechanisms by which DHEA and CR exert their effects , we examined two components of the apoptotic pathway , Bcl 2 and Bax . ^^^ Treatment of p 53 / animals with DHEA resulted in decreased Bcl 2 but not Bax mRNA levels in the thymus . ^^^ In contrast , CR did not change either Bcl 2 or Bax mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bax , and bcl XL proteins in azoxymethane induced rat colonic adenocarcinomas . ^^^ Using western blotting and immunochemical analysis , we investigated alterations in the expression of the apoptosis related proteins bcl 2 , bax , and bcl 10 in colonic adenocarcinomas induced by subcutaneous injection of azoxymethane ( AOM ) ( 15 mg / kg body weight weekly for 2 wk ) into male Sprague Dawley rats . ^^^ These findings indicate that the regulation of the apoptosis related proteins bcl 2 , bax , and bcl XL was altered in the AOM induced colonic neoplastic tissue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax / Bak lethality was suppressed by coexpression of Bcl 2 family members that are anti apoptotic in vertebrates , namely Bcl xL , Bcl 2 , Mcl 1 , and A 1 . ^^^ Furthermore , Bcl xL and Bcl 2 suppressed Bax toxicity by distinct mechanisms in yeast . ^^^ These inactive proteins bound to anti apoptotic members of the Bcl 2 family but not to Bax or Bak . ^^^ In contrast , most Bcl 2 family proteins that attenuated death bound to Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the dual effects ( proliferation and apoptosis ) of IL 10 on B cells , the expression of a panel of bcl 2 protoncogene family members , bcl 2 , bcl 10 , mcl 1 , and bax , was analyzed when B cells were activated by SAC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax alpha : Bcl 2 ratio modulates the response to dexamethasone in leukaemic cells and is highly variable in childhood acute leukaemia . ^^^ Bcl 2 over expression has been shown to inhibit apoptosis induced by a variety of stimuli , whereas a predominance of Bax alpha to Bcl 2 accelerates apoptosis upon apoptotic stimuli . ^^^ In a panel of leukaemia and lymphoma cell lines ( HL 60 , DoHH 2 , CEM C 7 , L 1210 and S 49 ) , the Bax alpha to Bcl 2 ratio as assessed by Western blot analysis correlated with sensitivity to dexamethasone treatment . ^^^ In bone marrow aspirates from patients with childhood acute lymphoblastic or myelocytic leukaemia ( ALL , n = 48 ; AML , n = 8 ) , the Bcl 2 and Bax alpha levels were highly variable , but well within the range found in the Bax alpha transfectants and in the established cell lines . ^^^ By contrast , Bax alpha : Bcl 2 was independent of any presenting feature and was largely dependent on Bax alpha levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Preablation biopsy specimens and prostatectomy specimens were immunohistochemically stained for apoptotic cells and for expression of apoptosis regulatory proteins Bcl 2 , Bax , Bcl 10 , and Bak . ^^^ In all 26 specimens , benign prostatic hyperplasia demonstrated increased expression of the Bcl 2 protein , but no change in the expression of Bax , Bcl 10 , and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cells growing attached to flasks appear to be relatively more resistant than suspension growing cells in spite of endogenous bcl 2 , bax , or bcl 10 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For cervical cancer and its precursors , data on apoptotic index , bcl 2 and Bax expression are presented and discussed in relationship to human papillomavirus expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of the expression of the human Bcl 2 family proteins Bax , Bak , Bcl 2 , and Bcl XL were examined in the fission yeast Schizosaccharomyces pombe and compared with Bax induced cell death in mammalian cells . ^^^ Bcl 2 also partially abrogated Bax mediated cytotoxicity in S . pombe , whereas a mutant of Bcl 2 ( Gly145Ala ) that fails to heterodimerize with Bax or block apoptosis in mammalian cells was inactive . ^^^ Taken together , these findings suggest that Bcl 2 family proteins may retain an evolutionarily conserved ability to regulate cell survival and death but also indicate differences in the downstream events that are activated by overexpression of Bax or Bak in divergent cell types . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , DEX mediated rescue from chemotherapy does not directly involve Bcl 2 family proteins since DEX failed to change the expression of Bcl 2 or Bax proteins and since bcl 2 gene transfer mediated cytoprotection was not redundant with the effects of DEX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed that NB 4 and NB 4 R1 cells differed with respect to the intracellular localization of Bcl 2 which showed a perinuclear localization in NB 4 R1 cells , while Bax was broadly expressed in the cytoplasm and to only a minor extent in the perinuclear area . ^^^ Therefore , the distinct intracellular localization of Bcl 2 and Bax was in general nonoverlapping . ^^^ Interestingly , the overexpression of Bcl 2 restored a pattern of uniform Bcl 2 labeling in the cytoplasm and , remarkably , the colocalization of Bcl 2 with Bax . ^^^ This work demonstrates that the ability of retinoid induced cells to undergo apoptosis depends on the level of expression and the functional interaction between Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic value of the expression of p 53 , bcl 2 , and bax oncoproteins , and neovascularization in patients with radically resected non small cell lung cancer . ^^^ PURPOSE : To assess the prognostic value of p 53 , bcl 2 , bax , and neovascularization in radically resected non small cell lung cancer ( NSCLC ) patients . ^^^ RESULTS : p 53 was not correlated to any patient or tumor characteristic , whereas bcl 2 showed higher expression in squamous cell carcinomas ( P < . 001 ) . bax expression was significantly related with male sex ( P = . 006 ) and adenocarcinoma type ( P = . 0013 ) . p 53 status , assessed with one monoclonal antibody ( MoAb ) , was not predictive for survival ; however , the combination of staining results obtained with two MoAbs identified the DO 7 / PAb1801+ tumors as those with the worst prognosis . bcl 2 expression was associated with longer survival in stage 1 patients ( P = . 0169 ) . ^^^ The combined group expressing p53+ ( PAb 1801 ) / bcl 2 had the worst survival in stage 1 patients ( P = . 034 ) and in the whole series in comparison with the other combinations of the two oncoproteins . bax expression alone had no influence on survival of stage 1 patients , but patients with bax+ / bcl 2 tumors had the worst prognosis ( P = . 02 in comparison with bax+ / bcl 2+ ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On mRNA or protein levels induction of pro and anti apoptotic gene products like fasL , bcl 2 , or bax with minor effects on fas / Apo 1 or bcl XL was observed under culture conditions with both IL 2 and IL 15 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Structural and functional complementation of an inactive Bcl 2 mutant by Bax truncation . ^^^ Previous work has shown that the death inhibiting family members Bcl 2 and Bcl xL form heterodimers with the death promoting homologue Bax and that certain site directed mutants of Bcl 2 and Bcl xL lose both biological activity and the ability to bind Bax . ^^^ To better understand the structural basis of heterodimer formation , we have used a yeast two hybrid assay to screen for mutants of Bax that regain the ability to bind to these inactive Bcl 2 ( G145A ) and Bcl xL ( G138A ) mutants . ^^^ This screen identified a series of C terminally truncated Bax molecules that contain complete BH 3 ( Bcl 2 homology domain 3 ) domains but that have lost BH 1 and BH 2 sequences . ^^^ These results indicate that while the Bcl 2 and Bcl xL mutants fail to bind full length Bax , they still retain a binding site for the critical BH 3 domain . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since phosphorylation / dephosphorylation reactions have been suggested to be involved in the regulation of Bcl 2 , we planned to investigate whether the expression of Bcl 2 , Bcl 10 ( L ) and Bax , a protein that antagonizes the antiapoptotic function of Bcl 2 , are regulated in myeloid leukemia cell lines ( K 562 , KU 812 and HL 60 ) treated with okadaic acid . ^^^ Our results indicate that exposure of all three leukemic cell lines to nanomolar concentrations of okadaic acid causes a loss of viability by activation of an apoptotic process accompanied by a marked decrease in the expression of Bcl 2 , Bcl 10 ( L ) and Bax at both mRNA and protein level , but not of c fos , vimentin and epsilon globin , ruling out a non specific effect of okadaic acid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results suggest that up regulation of the genes associated with the cell cycle and / or DNA replication , such as p 21 , cyclinG and gadd 45 rather than bax , fas , fasl and bcl 2 may be important for induction of apoptosis of this erythroleukemic cell line by p53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Suppression of nitric oxide induced apoptosis by N acetyl L cysteine through modulation of glutathione , bcl 2 , and bax protein levels . ^^^ In summary , our results suggest that the protective effect of LNAC may be linked to its inducement of increases in cellular GSH and bcl 2 protein levels and to its suppression of cellular bax protein in treated cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of bi transgenic K rasVal 12 10 TAgWt mice homozygous for wild type or null p 53 alleles established that the enhancement of apoptosis occurs through a p 53 independent mechanism , is not attributable to augmented proliferation or to an increase in abortive cell cycle reentry ( compared to TAgWt mice ) , and is not associated with detectable changes in the crypt villus patterns of expression of apoptotic regulators ( Bcl 2 , Bcl xL , Bak , and Bax ) or mediators of epithelial cell matrix interactions and survival ( e . g . , alpha5beta1 integrin and its ligand , fibronectin ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of apoptosis associated genes bax and bcl 2 in ovarian cancer . ^^^ In the present study , tumor tissue from 215 patients with ovarian cancer was immunohistochemically analysed for Bax and Bcl 2 expression . ^^^ In contrast , Bax expression ( 47 % ) was related to bad clinical outcome , especially in cases without concomitant Bcl 2 expression . ^^^ In patients with Bcl 2 positive / Bax negative tumors , overall survival was significantly longer ( p = 0 . 0379 ) than in patients with Bcl 2 and Bax negative tumors . ^^^ Respectively , expression of Bax without Bcl 2 expression was correlated with bad clinical outcome ( p = 0 . 033 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of Bax channel forming activity by Bcl 2 . ^^^ Bax , a pro apoptotic member of the Bcl 2 family , was shown to form channels in lipid membranes . ^^^ Thus , the pro apoptotic effects of Bax may be elicited through an intrinsic pore forming activity that can be antagonized by Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a member of the Bcl 2 multigene family , is known to promote apoptosis . ^^^ Cleavage of poly ( ADP ribose ) polymerase caused by nigericin was greatly attenuated in MN9D / Bax cells suggesting that , like Bcl 2 , Bax suppresses nigericin induced cell death by inhibiting the activation of cysteine proteases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further understand the underlying mechanism ( s ) for these findings as they relate to gene directed neural cell death , we studied the in situ expression of the Bcl 2 family of proteins , including the pro apoptosis gene product Bax , the anti apoptosis gene product Bcl 2 , and Bcl 10 . ^^^ In vitro studies using Western blot analysis demonstrated an up regulation in the levels of Bax , and phosphorylated ( i . e . inactive ) Bcl 2 in HIV 1 infected macrophages , and in LPS activated macrophages , relative to levels in virus negative unstimulated macrophages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the absence of an external death stimulus , p 53 and p 53 dependent genes , bcl 2 and bax , can not trigger apoptosis . ^^^ However , p 53 may enhance not only transcription of bax and repress bcl 2 , but also may upregulate the local renin angiotensin system , inducing the formation and secretion of angiotensin 2 from the cells . ^^^ To test this hypothesis , adult rat ventricular myocytes were infected with AdCMV . p53 , which resulted in downregulation of Bcl 2 , upregulation of Bax , and death of 34 % of the cells . ^^^ Thus , p 53 enhances the myocyte renin angiotensin system and decreases the Bcl 2 / Bax ratio in the cells , triggering apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 10 ( an antiapoptotic Bcl 2 family protein ) was not altered by bile duct ligation , whereas expression of Bax ( a proapoptotic Bcl 2 family protein ) increased slightly as determined by Northern and Western blot analyses . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , subsequent introduction of an activated c H ras oncogene into the c myc transfected CHST 8 cells resulted not only in escape from the growth arrest at 39 degrees C but also in complete inhibition of the phenobarbital inducible apoptosis along with de novo induction of the Bax antagonist , Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the BCL 2 protein family members , BAX , BAK , BAD , BCL xL , BCL xS , and BCL 2 , was measured ( by western blotting using specific antibodies ) in PC 12 cells before and during apoptosis induced by either H2O2 treatment or by serum deprivation and during rescue from apoptosis by nerve growth factor ( NGF ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , IL 4 and IGF 1 do not change expression of the apoptotic inducer , Bax , although they maintain high levels of Bcl 2 that coimmunoprecipitate with Bax . ^^^ Collectively , these data demonstrate that IL 4 and IGF 1 , like IL 3 , inhibit apoptosis in myeloid progenitors and maintain high levels of Bcl 2 / Bax heterodimers , suggesting that Bcl 2 is a critical convergence point in the signaling pathways used by IL 4 and IGF I . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 protection is not mediated by a negative effect on the induction of the p 53 responsive genes bax or waf 1 but it slows down at least two stages of apoptosis : decrease of mitochondrial membrane potential and subsequent morphological changes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax function independently to regulate cell death . ^^^ Using a genetic approach to address this question , we utilized gain and loss of function models of Bcl 2 and Bax and found that apoptosis and thymic hypoplasia characteristic of Bcl 2 deficient mice are largely absent in mice also deficient in Bax . ^^^ A single copy of Bax promoted apoptosis in the absence of Bcl 2 . ^^^ In contrast , overexpression of Bcl 2 still repressed apoptosis in the absence of Bax . ^^^ While an in vivo competition exists between Bax and Bcl 2 , each is able to regulate apoptosis independently . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High levels of the antiapoptotic Bcl 10 ( L ) or Bcl 2 , relative to the proapoptotic Bax , have been shown to inhibit HIDAC induced cleavage and activity of caspase 3 and apoptosis of the human acute myeloid leukemia HL 60 cells . ^^^ HIDAC treatment for 4 h also modestly increased the intracellular levels of free Bax relative to Bax bound to Bcl 2 and Bcl 10 ( L ) in HL 60 / neo but not in HL 60 / Bcl 10 ( L ) cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Remarkably , reentry of villus enterocytes to the cell cycle increases the radiosensitivity of the crypt epithelium without changing Bcl 2 , Bcl xL , Bak , or Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of apoptosis related proteins Bcl 2 and Bax in node negative breast cancer patients . ^^^ In this immunohistochemical study we investigated the expression of Bcl 2 and Bax apoptosis related proteins in node negative breast carcinomas . ^^^ Bcl 2 and to a lesser degree Bax expression were inversely related with distant metastases ( Pbcl 2 = 0 . 007 , Pbax = 0 . 03 ) . ^^^ Combined analysis of Bax / Bcl 2 expression in relation to clinical outcome showed that the absence of both factors was more strongly associated with the development of distant metastases ( P = 0 . 006 , Ptrend = 0 . 001 ) . ^^^ Our findings indicate that Bcl 2 and Bax apoptosis related proteins are good indicators of prognosis in node negative breast cancer patients , and their combined absence , suggestive of serious deregulation of the apoptotic process , may contribute to the biologic aggressiveness of the tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of members of the bcl 2 gene family in proliferative and secretory human endometrium : glandular epithelial cell apoptosis is associated with increased expression of bax . ^^^ To better understand the regulation of apoptosis in this paradigm of endocrine regulated cell turnover , we studied the expression of the cell death regulatory genes , bax , bcl 2 , and bcl 10 , in human proliferative and secretory endometria relative to the absence or presence of apoptosis . ^^^ Taken together , our results demonstrate a coordinated pattern of expression of bcl 2 gene family members in human endometrium during the menstrual cycle , with a shift toward greater levels of the proapoptosis protein , BAX , occurring in glandular epithelial cells during the secretory phase of the cycle . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related proteins such as Bcl 2 , Bax and Fas are expressed in the salvaged cardiomyocytes edging the infarct area . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The efficacy of anticancer drugs is determined by the ability to selectively sensitize tumor cells to apoptosis , which depends to a large extent from the expression of various oncogenes , such as bcl 2 , p 53 , bax , ras , c myc and others , and from endogenous factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The tumor suppressor p 53 and Bax ( Bcl 2 associated protein 10 ) are considered to be classical death promotors , which accumulate after toxic insults . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The onco proteins Bcl 2 and Bax and the scavenger proteins Cu / Zn superoxide dismutase ( SOD 1 ) and Mn superoxide dismutase ( SOD 2 ) are among the 40 50 proteins whose synthesis is altered by ( ) deprenyl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cAMP increasing reagents and butyrate also showed different effects on expression of members of the bcl 2 family ; PGE 2 decreased bcl 2 and bax levels , whereas butyrate increased the bcl 2 level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Cells of the human cervical carcinoma cell line C33A and normal human cervical epithelial cells were treated with 4HPR and analyzed for survival , induction of apoptosis , generation of reactive oxygen species , and expression of the apoptosis related proteins Bcl 2 and Bax . ^^^ Pyrrolidine dithiocarbamate , an oxygen radical scavenger , suppressed the rate of generation of reactive oxygen species and inhibited 4HPR induced apoptosis . 4HPR failed to modulate cellular levels of the Bcl 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After exposure to soluble CD 95 ligand , Jurkat T cells , but not Ag specific T cells , exhibit loss of BCL 2 and BCL 10 expression whereas BAX expression is not affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we examined expression patterns of the anti apoptotic proteins Bcl 2 and Bcl 10 and the cell death promoting protein Bax in retinae after crushing the optic nerve . ^^^ Thus , retinal ganglion cell death might be prevented by ablation of Bax protein in these cells , or by up regulation of Bax antagonists such as Bcl 2 or Bcl X . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These lesions also revealed expression of apoptosis promoting factors , such as bax and ICE , inducing cleavage of myofilaments , and of the apoptosis inhibiting proteins bcl XL and bcl 2 which neutralized high bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For example , bax , which can exist as a homodimer , is also able to form a heterodimer with bcl 2 . ^^^ A surexpression of bax in several cell lines allows to counteract the effect of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mutation of a tyrosine residue within the conserved N terminal BH 4 region had no effect on the ability of Bcl 2 or its closest homologs to enhance cell survival and did not prevent heterodimerization with death enhancing family members Bax , Bak , Bad and Bik . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It shares a conserved domain , BH 3 , with other pro apoptotic proteins , Bax , Bak , Bid , and Hrk , and certain anti apoptosis proteins such as Bcl 2 and Bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , one of the bcl 2 family genes , is expressed in a number of untransformed cell lines and various breast tissues , whereas only weak or no expression has been detected in breast cancer cell lines and malignant breast tissue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Three human colonic adenoma cell lines ( AA / C1 , RG / C2 and BH / C1 ) and one carcinoma cell line ( S / KS / FI ) were used to determine the effects of butyrate on the expression of bcl 2 , bax and bak to examine the possible role of these proteins in the induction of apoptosis . ^^^ RG / C2 and BH / C1 cells express p 26 bcl 2 and butyrate treatment decreased p 26 bcl 2 levels in association with apoptosis , whereas bax and bak levels remained constant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Granule cells from mice in which Bax , a proapoptotic Bcl 2 family member , had been deleted , did not undergo apoptosis in 5 mM potassium , yet did undergo an excitotoxic cell death in response to stimulation with 30 or 100 microM NMDA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , p 53 dependent transcription of bax was repressed 4 fold ( P < . 05 ) by bcl 2 , suggesting a tentative link between p 53 mediated apoptosis and the protective properties conferred by bcl 2 in ventricular myocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For example , apoptotic death depends on the balance of Fas , Fas ligand , nuclear factors ( such as bcl 2 , bax , and myc ) , cytokines , neuropeptides , and cell membrane interactions with extracellular matrix . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Apo 1 / Fas ( CD 95 ) , Bcl 2 , Bax and Bcl 10 in myeloma cell lines : relationship between responsiveness to anti Fas mab and p 53 functional status . ^^^ We studied eight myeloma cell lines for the presence of Bcl 2 , which inhibits apoptosis , of Bax , which counteracts Bcl 2 , of Bcl 10 ( L ) and Bcl 10 ( S ) , which act in an anti and pro apoptotic fashion , respectively , and of Apo 1 / Fas , which induces programmed cell death , when activated by the Apo 1 / Fas ligand or the relevant monoclonal antibody ( mab ) . ^^^ All cell lines constitutively expressed homogenous amounts of Bcl 2 , but displayed different amounts of Bax and Bcl 10 proteins . ^^^ The relative expression levels of Apo 1 / Fas correlated with that of Bax , but not with that of Bcl 2 or Bcl 10 subtypes . ^^^ Furthermore , the effectiveness of the Apo 1 / Fas mab was associated with the relative expression levels of the Apo 1 / Fas and with that of the Bax antigen , but not with that of the Bcl 2 and Bcl 10 antigens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Comparison of the ion channel characteristics of proapoptotic BAX and antiapoptotic BCL 2 . ^^^ Both BAX and BCL 2 insert into KCl loaded vesicles in a pH dependent fashion and demonstrate macroscopic ion efflux . ^^^ BAX and BCL 2 each form channels in artificial membranes that have distinct characteristics including ion selectivity , conductance , voltage dependence , and rectification . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our data also indicate the possibility that a cell death program dependent on the bcl 2 family exists , because of the potential involvement of p 53 , bcl XS and Bax in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We compared the apoptotic threshold and expression of the Bcl 2 protein family members , Bcl 2 , Bcl XL , and Bax , in two human cell lines : 1 ) nonmalignant human cholangiocytes immortalized by transfection with the simian virus 40 ( SV 40 ) large T antigen ; and 2 ) a malignant human cholangiocarcinoma cell line . ^^^ Bcl 2 , Bcl 10 long , and Bax protein expression were evaluated by immunoblot analysis , and Bcl 2 expression was modulated using antisense technology . ^^^ Although expression of Bcl 10 long and Bax protein were similiar in the two cell lines , Bcl 2 protein expression was 15 fold greater in malignant than in nonmalignant cholangiocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a pro apoptotic member of the Bcl 2 family , does not associate with the complex ; however , it prevents Bcl 2 from doing so . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein expression of Fas / APO 1 or bcl 2 , and messenger RNA ( mRNA ) expression of bcl 2 , bcl xL , bax , bak , Fas / APO 1 , Fas ligand ( Fas L ) , c myc , mad , or max were determined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of several other bcl 2 family members ( bcl 10 , ich 1 , bax , bag , and bak ) and retinoid receptors ( RARalpha , RXRalpha , and RXRbeta ) was not affected by treatment with RAR and / or RXR activating retinoids ; RARbeta RNA was undetectable before and after retinoid treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Homology modeling of Bcl 2 and Bax , based on the Bcl xL structure , suggests that Bax has the strongest potential for membrane insertion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the mechanisms of apoptosis during differentiation of HL 60 cells induced by NO , the endogenous levels of Bcl 2 and Bax were assessed by immunoblotting . ^^^ Treatment of cells with NOC 18 slightly reduced the level of Bcl 2 followed by Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of treatment with hyperthermia and verapamil on the expression of apoptosis associated proteins including Bcl 2 , p 53 , bax , and c Myc was studied by Western blot analysis . ^^^ Our experiments have shown that HT 29 cells constitutively express apoptosis promoting proteins , such as Bax and c Myc , while they fail to produce Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 related protein bax could be found at days 2 and 4 in similar expression , was considerably up regulated at day 7 , but was not regulated by CD 95 agonistic antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 counters apoptosis by Bax heterodimerization dependent and independent mechanisms in the T cell lineage . ^^^ The effect of the cell death inhibitor Bcl 2 in relation to its capacity to dimerize with apoptosis promoter Bax or its homologs at their physiological expression levels was explored in the T cell lineage . ^^^ Transgenic mice expressing a BH 1 mutant Bcl 2 ( Bcl 2 mI 3 ) , which fails to heterodimerize with proapoptotic members of the Bcl 2 family , such as Bax , were generated . ^^^ Thus , within the T cell lineage Bcl 2 can inhibit apoptosis independent of its association with Bax or its homologs ; yet , above a threshold level of their physiologic proapoptotic activity , the capacity of Bcl 2 to heterodimerize with Bax or its homologs appears essential for it to counter cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase dependent apoptosis of COS 7 cells induced by Bax overexpression : differential effects of Bcl 2 and Bcl xL on Bax induced caspase activation and apoptosis . ^^^ In this study , we found that the overexpression of the Bcl 2 family member Bax induced apoptotic cell death in COS 7 cells through the activation of CPP 32 ( caspase 3 ) like proteases that cleaved the DEVD tetrapeptide . ^^^ We also found that the Bax induced apoptosis of COS 7 cells was suppressed by Bcl xL and Bcl 2 , though both Bcl xL and Bcl 2 similarly prevented etoposide induced apoptosis in COS 7 cells . ^^^ In addition , Bcl xL inhibited the activation of caspase 3 like proteases accompanying Bax induced COS 7 cell death but Bcl 2 did not . ^^^ These results indicate that the caspase activation is essential for Bax induced apoptosis , and that the ability of Bcl 2 and Bcl xL to prevent the Bax induced caspase activation and apoptosis in COS 7 cells could be differentially regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantification of Bax / Bcl 2 ratios in peripheral blood lymphocytes , monocytes and granulocytes and their relation to susceptibility to anti Fas ( anti CD 95 ) induced apoptosis . ^^^ The homologous Bcl 2 and Bax proteins have opposing effects , with Bcl 2 extending cellular survival and Bax promoting cell death following an apoptotic stimulus . ^^^ We determined Bcl 2 to Bax expression ratios in peripheral blood lymphocytes , monocytes and granulocytes and related them to the susceptibility of these cells to anti Fas ( anti CD 95 ) induced apoptosis . ^^^ Here , we show that Bax / Bcl 2 ratios are high in granulocytes and relatively low in monocytes and lymphocytes . ^^^ It is concluded that the balance between Bcl 2 and Bax forms an apoptotic rheostat , which seems to determine sensitivity to apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No changes in expression of the apoptosis genes bcl 2 and bax , nor the drug resistance genes mdr 1 , mrp , or O 6 alkyltransferase was found in any of the cell types ( de novo , rho ( ) , or cybrid ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Tpo treatment induced an extranuclear buildup and greatly weakened the DNA binding capacity of p 53 . p 53 specific antisense oligonucleotide treatment recapitulated the effects of Tpo treatment on the levels of Bax , Mdm 2 , and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
But while the primary trigger of cell death varies between tissues , underlying cellular mechanisms are more conservative and cell death / survival genes , such as bcl 2 , bax and others that are expressed in other tissues , play important roles in the reproductive system too . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Several of these genes , including bcl 2 , bcl 10 , and bax , share homology at the amino acid level in the BH 1 and BH 2 domains , through which they also interact . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a proapoptotic member of the Bcl 2 protein family . ^^^ Of the 7 Bax immunonegative lymphomas , all also scored low ( < or = 10 % immunostained tumor cells ) for Bcl 2 expression , whereas 78 of the 106 ( 74 % ) Bax immunopositive tumors had low Bcl 2 expression . ^^^ When combined with Bcl 2 immunostaining data , Bax provided additional prognostic information . ^^^ Among patients with Bcl 2 low expressing DLCLs , for example , Bax immunonegativity was associated with lower 8 year relapse free survival ( RFS ; 29 % 5 61 % ; P < . 01 ) and lower 8 year overall survival ( OS ; 29 % 5 63 % ; P < . 05 ) , suggesting that absence of Bax protein connotes a more aggressive phenotype when Bcl 2 protein is also not expressed at high levels . ^^^ In contrast , low Bax expression was associated with improved 8 year disease free survival ( 52 % 5 16 % ; P < . 02 ) , RFS ( 47 % 5 11 % ; P < . 02 ) , and OS ( 64 % 5 11 % ; P < . 01 ) in patients whose tumors expressed Bcl 2 at high levels , suggesting that the combination of high levels of Bax and Bcl 2 expression is more deleterious than high levels of Bcl 2 expression alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protooncogene protein , Bcl 2 , protects cells from apoptosis and ensures their survival in vitro by inhibiting the action of the apoptosis inducer , Bax . ^^^ In contrast , Bax immunoreactivity changed little during chondrocyte terminal evolution , and its subcellular distribution mirrored that of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consequently , the E1B 19K and Bcl 2 proteins bind to and inhibit the cellular death inducing proteins Bax , Bak and Nbk / Bik . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BH 3 domains from the pro apoptotic proteins Bax and Bak , but not the BH 3 domain of the anti apoptotic protein Bcl 2 , induced apoptosis in this system , as determined by the rapid activation of specific apoptotic proteases ( caspases ) and by DNA fragmentation . ^^^ These results support a model in which pro apoptotic proteins , such as Bax and Bak , bind to Bcl 2 via their BH 3 domains , inactivating the normal ability of Bcl 2 to suppress apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a member of the Bcl 2 protein family , accelerates apoptosis by an unknown mechanism . ^^^ Bax has been recently reported to be an integral membrane protein associated with organelles or bound to organelles by Bcl 2 or a soluble protein found in the cytosol . ^^^ To explore Bcl 2 family member localization in living cells , the green fluorescent protein ( GFP ) was fused to the NH 2 termini of Bax , Bcl 2 , and Bcl XL . ^^^ Confocal microscopy performed on living Cos 7 kidney epithelial cells and L 929 fibroblasts revealed that GFP Bcl 2 and GFP Bcl XL had a punctate distribution and colocalized with a mitochondrial marker , whereas GFP Bax was found diffusely throughout the cytosol . ^^^ Photobleaching analysis confirmed that GFP Bax is a soluble protein , in contrast to organelle bound GFP Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , as previously shown for BAX , BAK , BCL 2 , and BCL XL , the BH 3 domain of BAD is required for its dimerization with other BCL 2 family proteins . ^^^ BAD was further analyzed for its ability to bind to various mutants of BCL 2 and BCL XL that have lost the ability to bind BAX and BAK , some of which retain biological activity and some of which do not . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , we examine the biological mechanisms underlying 5 FU or the combination of 5 FU plus IFN gamma , using the colorectal carcinoma cell line , COLO 201 , 5 FU and IFN gamma independently or additively induced apoptosis in COLO 201 in a dose and time dependent manner , which was correlated with the down regulation of Bcl 2 and the up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
P 53 , MDM 2 , BAX and BCL 2 and drug resistance in chronic lymphocytic leukemia . ^^^ Most antitumor agents exert their cytotoxic effect through the induction of apoptosis , and this process may be mediated through an elevation in p 53 protein , with a subsequent increase in bax and decrease in bcl 2 . p 53 also increases mdm 2 expression and mdm 2 may then bind and inactivate p 53 . ^^^ The protein levels of bax and bcl 2 were measured in CLL cells from 25 patients , and were found to be higher in leukemic cells than in normal B cells . ^^^ The bcl 2 levels varied three fold , the bax levels fifteen fold , and the bax : bcl 2 ratios ranged from 0 . 44 to 2 . 91 . ^^^ However , no correlation was observed between drug sensitivity to CdA , F ara A , or CLB and the cellular levels of mdm 2 mRNA , or the protein levels of bax or bcl 2 , or the bax : bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax , a pro apoptotic member of the Bcl 2 family , in esophageal squamous cell carcinoma . ^^^ In invasive carcinomas , Bax immunoreactivity was inversely correlated with Bcl 2 expression ( p = 0 . 0243 ) and decreased continuously with decreasing tumor differentiation ( p = 0 . 0011 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pattern of endogenous expression levels of Bax , Bcl 2 , Bcl 10 and Bak , which was not modulated by cisplatin treatment , demonstrated that these Bcl 2 family proteins are not involved in drug induced apoptosis in the TGCT cell lines . ^^^ We conclude that the cisplatin induced apoptotic pathway in TGCT cell lines might be p 53 independent and is probably not associated with differences in the Bcl 2 / Bax rheostat . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we demonstrate that expression of galectin 3 in human breast carcinoma BT 549 cells inhibits cis diamminedichloroplatinum ( cisplatin ) induced poly ( ADP ribose ) polymerase degradation and apoptosis , without altering Bcl 2 , Bcl 10 ( L ) , or Bax expressions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We postulated that Mycobacterium tuberculosis could trigger the apoptotic pathway in macrophages , resulting in death of the microorganism by modulating the expression of bcl 2 , bax , bcl xL , and bcl xS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of the bcl 2 related bax gene , showed a similar pattern as bcl 2 ; a 60 % increase was initially measured , but after 1 . 5 and 3 hr , bax transcripts were 80 % and 70 % respectively , of those found in untreated cells . ^^^ It is concluded that 3 deazaadenosine initiated apoptosis affects c myc , bcl 2 , bax and bcl x 1 mRNA levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HL 60 / Bcl 2 cells displayed a 5 fold increase in Bcl 2 protein compared with empty vector counter parts ( HL 60 / pCEP4 ) but comparable levels of Bax , Mcl 1 , and Bcl xL . ^^^ Last , phosphorylated Bcl 2 failed to display decreased binding to the proapoptotic protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Topoisomerase 1 inhibitors for human malignant glioma : differential modulation of p 53 , p 21 , bax and bcl 2 expression and of CD 95 mediated apoptosis by camptothecin and beta lapachone . ^^^ Acute cytotoxicity induced by both drugs is apoptotic by electron microscopy , but not blocked by inhibitors of RNA or protein synthesis and not associated with changes in the expression of bcl 2 , bax , p 53 , p 21 or GADD 45 proteins . ^^^ LN 229 cells which have partial p 53 wild type activity show enhanced expression of p 53 , p 21 and bax protein , whereas bcl 2 levels decrease , after exposure to camptothecin . ^^^ In these cells , p 53 levels do not change and p 21 is not induced . bax accumulation in T98G cells is induced by both drugs , with bcl 2 levels unaltered . ^^^ Thus , both drugs have potent anti glioma activity that may be mediated by enhanced bax expression but is not inhibited by ectopic bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fragmentation of DNA was quantitated , and p 53 , bax , and bcl 2 expression was analyzed at various times by the Western blot method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These apoptosis hot spots during catagen development correlated largely with a down regulation of the Bcl 2 / Bax ratio but only poorly with the expression patterns of interleukin 1beta converting enzyme , p55TNFR , and Fas / Apo 1 immunoreactivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we demonstrate that , in addition to the previously identified A 1 , four other members of the Bcl 2 family , Bcl 2 , Mcl 1 , Bcl 10 ( L ) , and Bax , are expressed in endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl xL overexpression restricts heat induced apoptosis and influences hsp 70 , bcl 2 , and Bax protein levels in FL5 . 12 cells . ^^^ The current study , using control and bcl xL overexpressing IL 3 dependent FL5 . 12 cells , compared the effects of 1 h of acute heat stress ( 42 degrees C ) followed by 1 , 4 , and 8 h recovery ( 37 degrees C ) on hsp 70 , bax , bcl 2 , and bcl xL protein levels and apoptosis . ^^^ Immunoblotting revealed a time dependent increase in hsp 70 protein levels following 1 h of heat stress in control , but not bcl xL overexpressing cells . bcl 2 protein levels were lower in bcl xL overexpressing cells than in controls , but decreased in both cell lines after heat stress . bax protein levels in bcl xL overexpressing cells were decreased approximately 80 % below baseline levels 1 h post heat shock . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , there was no apparent correlation of the steady state level of the apoptosis regulating proteins , Bcl 2 , Bcl XL , Bax and Ich 1 , with tumorigenicity of the prostate cells xenografted in nude mice , aggressiveness of tumors grown in nude mice , and induction of apoptosis by 9NC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECT : Genes known to be involved in the regulation of apoptosis include members of the bcl 2 gene family , such as inhibitors of apoptosis ( bcl 2 and bcl xl ) and promoters of apoptosis ( bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Coincidentally , transcriptional induction of bcl 2 and suppression of bax are also observed in MEQ transformed Rat 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , the Bcl 2 family member , Bcl 10 ( L ) also blocked staurosporine induced cell death in MN9D cells whereas overexpression of Bcl 10 ( S ) or Bax did not . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax and bcl 2 proteins , regulators of programmed cell death , in human brain tumors . ^^^ Expression of Bcl 2 , a programmed cell death ( PCD ) suppressing molecule , and Bax , the Bcl 2 related PCD accelerating protein was investigated in varied human brain tumors . ^^^ Thirty six cases of human brain tumors comprising 4 astrocytomas , 3 anaplastic astrocytomas , 4 glioblastomas multiforme , 5 medulloblastomas , 1 ependymoma , 2 choroid plexus papilloma , 1 ganglioglioma , 1 central neurocytoma , 4 meningotheliomatous meningiomas , 3 transitional meningiomas , 4 fibroblastic meningiomas , 3 acoustic neurinomas and 1 craniopharyngioma were analyzed for the localization of Bax and Bcl 2 proteins . ^^^ No relationship between the degree of the histological malignancy and the presence of Bax or Bcl 2 proteins was found in varied human brain tumors . ^^^ Our findings indicate that the expression pattern of Bax and Bcl 2 may reflect histogenetic difference of each type of brain tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax and Bcl 2 protein in the gerbil hippocampus following transient forebrain ischemia and its modification by phencyclidine . ^^^ To determine the effect of phencyclidine ( a noncompetitive NMDA receptor antagonist ) on expression of Bax and Bcl 2 ( apoptosis regulating proteins ) in gerbil hippocampus after transient forebrain ischemia , brain sections were immunohistochemically evaluated 48 , 72 , 96 h and 7 days following ischemia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alterations in Bcl 2 / Bax protein levels in platelets form part of an ionomycin induced process that resembles apoptosis . ^^^ By PCR , we have demonstrated mRNA expression of bcl 2 , bax , and p 53 in highly purified non stimulated platelets . ^^^ A side scatter shift and a decrease in the Bcl 2 / Bax protein ratio were observed by flow cytometry analysis after stimulation with ionomycin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results demonstrated that ( 1 ) Fas antigen ( exons 1 > 2 or 3 > 7+ ) , Fas ligand , c myb , c myc , bcl 2 , Bax , p 53 , and androgen receptor ( AR ) mRNAs are present in exocrine tissues of MRL / lpr mice ; ( 2 ) the amounts of c myb , c myc , bcl 2 , p 53 , and AR mRNA are higher ( P < 0 . 05 ) and the level of Fas antigen ( exons 1 > 2 ) mRNA is lower ( P < 0 . 05 ) in lacrimal glands of female compared to male mice . ^^^ In contrast , the content of c myb and p 53 mRNA is greater ( P < 0 . 05 ) in submandibular tissues of female relative to those of male mice ; and ( 3 ) testosterone or cyclophosphamide treatment led to a significant ( P < 0 . 05 ) decline in the mRNA levels of c myb , bcl 2 , and / or AR , but an increase ( P < 0 . 05 ) in the mRNA amount of Bax , in lacrimal , but not in salivary , glands of female mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have selected bcl 10 gene expression for study because there is increasing evidence that proteins encoded by the bcl 2 gene family ( bcl 2 , bcl 10 , bax etc ) play a role in the regulation of programmed cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The distribution of the cell death associated gene products , bcl 2 , bax , and interleukin 1 beta converting enzyme , indicates that although these proteins are segmentally distributed in the dermomyotome and in the rostrodorsal quadrant of the sclerotome , their patterns are not directly correlated with the distribution of dying cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This line , which expresses p glycoprotein and serves as a model of multidrug resistance in multiple myeloma cells , demonstrated an up regulated expression of BCL 10 L , which was relatively specific , in that BCL 2 or BAX expression was not altered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , PGE 2 inhibits programmed cell death caused by SC 58125 and induces Bcl 2 expression , but did not affect Bcl 10 or Bax expression in human colon cancer ( HCA 7 ) cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax and Bcl 10 expression following hypoxia ischemia in the infant rat brain . ^^^ Expression of Bcl 2 , Bax and Bcl 10 was examined in control and hypoxic ischemic rats using immunohistochemistry and Western blotting . ^^^ Bcl 2 , Bax and Bcl 10 immunoreactivity decreased in necrotic cells , but about 60 % of cells with apoptotic like morphology and cells with granular chromatin degeneration were stained with antibodies to Bcl 2 , Bax or Bcl 10 . ^^^ Western blots showed a band at about p 28 and p 21 , respectively for Bcl 2 and Bax in control and hypoxic ischemic pups at 6 , 12 and 24 h . ^^^ These results suggest that Bcl 2 , Bax , Bcl xL and Bcl xS do not play a leading role in the fate of damaged nerve cells following a severe hypoxic ischemic insult of the developing brain . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Changes in the expression of Bcl 2 and Bax and their transcriptional regulator , p 53 , were determined by Western blot analysis in myocytes isolated from dogs affected by pacing induced heart failure . ^^^ In addition , apoptosis was measured by confocal microscopy , which allowed the simultaneous detection of chromatin alterations and DNA damage . p 53 DNA binding activity to the bax promoter was increased in nuclear extracts from myocytes obtained from failing hearts , and this response was associated with enhanced expression of Bax protein , 52 % , and attenuation of Bcl 2 , 92 % . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of Bcl 2 , Mcl 1 , Bcl xL , and Bax each varied over a more than 10 fold range in different pretreatment leukemia specimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nevertheless , there are also anti apoptotic strategies in muscle fibers manifested by expression of the bax antagonist bcl 2 which is able to neutralize high bax levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the constitutive expression of HIV 1 vpr resulted in the upregulation of bcl 2 , an oncogene endowed with antiapoptotic activities , and in the downmodulation of bax , a proapoptotic factor of the bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study the molecular mechanisms of apoptosis mediated by overexpression of p 21 gene , the protein levels of bax , a promoter of apoptosis , and bcl 2 , an inhibitor of apoptosis , were also measured by Western blotting . ^^^ Overexpression of p 21 gene significantly increased protein of bax ( P < . 05 ) , whereas transfection of p 21 gene did not alter bcl 2 protein . ^^^ Importantly , the ratio of bax to bcl 2 was significantly increased in VSMC transfected with human p 21 vector as compared with control vector ( P < . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the clinical relevance of these laboratory studies , a group of 116 paraffin embedded non Hodgkin ' s lymphoma ( NHL ) biopsy specimens ( Working Formulation Groups D H , and J ) from a cooperative group study of cellular DNA content were analyzed for the 14 ; 18 translocation using polymerase chain reaction ( PCR ) based methods and , if sufficient tissue remained , for BCL 2 and BAX expression by immunohistochemistry . ^^^ BCL 2 expression was inversely associated with proliferative activity ( P = . 001 ; n = 41 ) , but there was no association between staining for Bax and % S phase . ^^^ Ploidy was not associated with either BCL 2 or BAX expression . ^^^ High levels of BCL 2 or BAX expression occurred independently of t ( 14 ; 18 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we investigated what effect the differentiation agent butyrate had on the cellular levels of the apoptosis regulators BCL 2 , BCLX ( L ) and BAX and on radiation induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential regulation of Bax , Bcl 2 , and Bcl 10 proteins in focal cortical ischemia in the rat . ^^^ Double staining for each of the Bcl 2 family proteins and TUNEL revealed that DNA strand breaks and nuclear fragmentation seen in cells located in the lesion core were often associated with increased levels of Bax , but not with elevated Bcl 2 or Bcl 10 protein levels , suggesting a role for Bax in the induction of apoptotic death in these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax mRNA levels increased with relative constitutive expression of bcl 2 mRNA levels . ^^^ This study shows that mitotic spindle inhibitors can induce apoptosis in fludarabine resistant malignant B 1 cells by altering levels of bax / bcl 2 ratio and BSAP which play different roles in cell cycle regulation and apoptosis induction . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relationship between nitrogen mustard drug resistance in B cell chronic lymphocytic leukemia ( B CLL ) and protein expression of Bcl 2 , Bax , Bcl 10 and p 53 . ^^^ Several genes have been implicated in the regulation of apoptosis including bcl 2 , bax , bcl 10 and p 53 . ^^^ Using Western blot analysis , we examined the levels of Bcl 2 , Bax , Bcl 10 and p 53 protein expression and determined whether the levels of these proteins correlated with in vitro drug resistance in CLL patients ' lymphocyte samples . ^^^ Our investigations suggest that in CLL , NM drug resistance develops without any detectable alteration of Bcl 2 , Bax or Bcl 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , SNAP treatment did not change protein levels of Bcl 2 and Bax . ^^^ These results suggest that SNAP induced p 53 sensitive apoptosis is enhanced by Bcl 2 reduction , and that Bcl 2 and Bax may act downstream of p 53 in SH SY5Y cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the centre of the lesion , Bax protein increased and Bcl 2 and Bcl 10 proteins decreased after loss of microtubule associated protein 2 antigenicity occurred , but at the border of the lesion , the former changes preceded loss of microtubule associated protein 2 antigenicity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the expression of three members of the Bcl 2 protein family , Bcl 2 , Bcl 10 , and Bax , in a selection of senile and DS related AD patients as well as in controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 prolongs cell survival after Bax induced release of cytochrome c . ^^^ Here we study these molecular interactions during apoptosis induced by the protein Bax , a pro apoptotic homologue of Bcl 2 . ^^^ Unexpectedly , Bcl 2 also fails to prevent Bax induced cytochrome c release , although it co localizes with Bax to mitochondria . ^^^ Cells overexpressing both Bcl 2 and Bax show no signs of caspase activation and survive with significant amounts of cytochrome c in the cytoplasm . ^^^ These findings indicate that Bcl 2 can interfere with Bax killing downstream of and independently of cytochrome c release . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and close homologues such as Bcl xL promote cell survival , while other relatives such as Bax antagonize this function . ^^^ BH 4 of Bcl 2 could be replaced by that of Bcl 10 without perturbing function but not by a somewhat similar region near the N terminus of Bax . ^^^ Deletion of BH 4 rendered Bcl 2 ( and Bcl xL ) inactive but did not impair either Bcl 2 homodimerization or ability to bind to Bax or five other pro apoptotic relatives ( Bak , Bad , Bik , Bid or Bim ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax proteins in Lewy bodies from patients with Parkinson ' s disease and Diffuse Lewy body disease . ^^^ Double labelling immunohistochemistry of Bcl 2 and Bax , and ubiquitin ( as a marker of Lewy bodies ) was examined in the brains of patients with Parkinson ' s disease and Diffuse Lewy body disease to learn about possible modifications of protein expression and the presence of Lewy bodies . ^^^ Bcl 2 and Bax immunoreactivities were observed in Lewy body bearing and non Lewy body bearing neurons in patients with parkinsonism . ^^^ These results show that Bcl 2 and Bax are probably not implicated in Lewy body formation and that the presence of Lewy bodies does not have a direct impact on the expression of Bcl 2 and Bax proteins in individual neurons . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The survival and proliferation inducing activity of thiol compounds in the presence of catalase , pyruvate and BCS was not associated with induction of BCL 2 or BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also found that the expression of p 53 , p21waf1 / cip1 , Bcl 2 , Bax , Bcl xL , Bad and cyclins D 1 , E , A and B did not show any significant changes following c Myc induction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased apoptosis of T cell subsets in aging humans : altered expression of Fas ( CD 95 ) , Fas ligand , Bcl 2 , and Bax . ^^^ We analyzed the expression of genes promoting apoptosis ( fas / fasL1 and bax ) and those inhibiting apoptosis ( bcl 2 and bcl xL ) in lymphocytes from aging and young subjects at the protein level , using flow cytometry / Western blotting , and at the mRNA level , using quantitative PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Considering ( 1 ) that phosphorylation of bcl 2 interferes with its heterodimerization with bax and ( 2 ) the inhibition of CD 95 mediated apoptosis by bcl 2 , we propose that taxol sensitizes malignant glioma cells to CD 95 ligand by increasing the functional bax / bcl 2 rheostat in favour of bax and thus cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both cytokines also increased the COLO 201 sensitivity to anti Fas antibody , resulting from the down modulation of Bcl 2 and the up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic population in the cells expressing Bax 112 192 was not decreased by co expression of Bcl 2 or Bcl XL , while Bcl 2 or Bcl XL suppressed apoptosis in the cells expressing native Bax . ^^^ Therefore , Bax induces apoptosis by its own activity without blocking the anti apoptotic activity involved in Bcl 2 or Bcl XL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The biopsies were assessed for regressive morphology , apoptotic index by morphological criteria , nuclear area , and immunoreactivity ( IR ) for Ki 67 , p 53 , bcl 2 , bax and Fas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the Bcl 2 family proteins Bax , Mcl 1 , Bcl 2 , and Bcl xL , was examined in human peripheral blood eosinophils or in umbilical cord blood derived eosinophils . ^^^ Incubation of both eosinophil types for 1 to 3 days in a cytokine deprived medium led to apoptosis , without changes in the expression of Bax , Mcl 1 , Bcl 2 , or Bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Susceptibility to induction of apoptosis is regulated by the homologous Bcl 2 and Bax proteins . ^^^ Expression of Bcl 2 and Bax is regulated by p 53 , which down regulates expression of Bcl 2 , while simultaneously up regulating expression of Bax . ^^^ Tubular expression of p 53 , Bcl 2 and Bax was studies by immunohistochemistry . ^^^ Compared to control renal tissue , acute renal allograft rejection was , furthermore , associated with a shift in the ratio of Bcl 2 to Bax in favour of Bax in tubular epithelia and increased expression of p 53 in tubular nuclei . ^^^ In accordance , the shift in the ratio of Bcl 2 to Bax in favour of Bax indicates increased susceptibility of tubular epithelia to induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate IGF 1 actions on apoptosis of H9C2 cardiac muscle cells , we have defined the effects of IGF 1 on Bcl 2 , Bax , caspase 3 , DNA fragmentation , and cell survival . ^^^ The abundance of Bcl 2 and Bax was determined with immunoblotting , and the activities of caspase 3 were assayed with the labeled substrate DEVD p nitroanilide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered bcl 2 and bax expression and intracellular Ca2+ signaling in apoptosis of pancreatic cells and the impairment of glucose induced insulin secretion . ^^^ Apoptosis is the process of cellular self destruction , and genes such as bcl 2 and bax are known to inhibit and promote apoptosis , respectively . ^^^ In this study , we show that apoptosis can be induced in pancreatic beta cell lines , and we investigate the apoptotic pathways through the bcl 2 and bax genes and intracellular Ca2+ . ^^^ KCl , BayK , A 23187 , and ionomycin elicit an elevation of cytosolic / nuclear Ca2+ , which , however , is insufficient to evoke apoptosis or to alter bcl 2 or bax mRNA expression in MIN 6 cells . ^^^ The extracellular Ca2+ chelators , EGTA and 1 , 2 Bis ( 2 aminophenoxy ) ethane N , N , N ' , N ' tetraacetic acid , tetrapotassium salt , hydrate , evoke apoptosis and also alter the ratio of bcl 2 to bax mRNA and protein concomitantly with the depletion of cytosolic / nuclear Ca2+ . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our data indicate that neither the nonresponsiveness nor the susceptibility to CTLA 4 of antigen experienced CD 4 T cells are related to the expression levels of Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and cell proliferation were determined by immunocytological techniques and the markers p 53 , p21WAF / Cip , Bax , Bak , Bcl 2 , cyclin D 1 and PCNA , and also screened for signal transduction indicators such as c H ras , c fos and raf 1 . ^^^ There were quantitative differences in the activation of p 53 , Bax , p21WAF and Bcl 2 in HHV 6 infected CBC as compared to HHV 7 infection supporting the apoptosis cycle . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have studied the expression of apoptosis regulating genes bcl 2 and bax in neuroendocrine gut tumors . ^^^ With regard to clinical outcome , the scores ( mean + / SEM ) of immunohistochemical staining of bcl 2 and bax were 1 . 77 + / 0 . 25 and 4 + / 0 . 22 for patients with stable disease and , 0 . 54 + / 0 . 28 and 4 . 68 + / 0 . 21 for patients with progressive disease . ^^^ The scores of bcl 2 and bax staining for IFN alpha treated patients were 1 . 96 + / 0 . 35 and 4 . 12 + / 0 . 31 and for untreated patients were 0 . 5 + / 0 . 25 and 4 . 5 + / 0 . 21 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 are functionally antagonistic proteins which control apoptosis , whose expression in human tumours could be of prognostic value . ^^^ We evaluated Bax and Bcl 2 expression in 239 breast carcinomas ( 99 N 0 , 140 N1 / 2 ) with long term follow up ( median 79 months , range 11 140 ) in relation to clinico pathologic parameters , clinical outcome , adjuvant therapy and expression of oestrogen receptor protein and p 53 . ^^^ High Bax expression was associated with positive nodal status ( p = 0 . 03 ) and high Bcl 2 expression ( p = 0 . 01 ) and was more frequent in high grade tumours . ^^^ Since the apoptotic rate of a tumour is influenced by the ratio Bcl2 / Bax , we investigated the combined effects of Bax and Bcl 2 expression in relation to clinical outcome . ^^^ However , no differences in survival were seen in the Bcl 2 negative and Bcl 2 positive groups when they were subdivided on the basis of the level of Bax expression and vice versa . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis of primary adenocarcinomas revealed expression of the anti apoptotic proteins Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and BAG 1 , as well as the pro apoptotic proteins Bax , Bak , and CPP 32 , in at least 2 of the 3 tumors examined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased ratio of bcl 2 / bax expression is associated with bovine leukemia virus induced leukemogenesis in cattle . ^^^ Because the ratio of Bcl 2 to Bax is believed to predetermine the susceptibility to a given apoptotic stimulus , the relative expression of the genes encoding these oncoproteins was evaluated in cattle naturally infected with BLV . ^^^ In BLV infected cattle an increase in the ratios of bcl 2 / bax mRNA and protein expression correlated with advancing stages of disease . ^^^ These findings suggest that in addition to the maintenance of BLV associated hematopoietic malignancies , the reciprocal expression of Bcl 2 / Bax may modulate the induction of B cell expansion typical of BLV disease progression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alteration of proteins regulating apoptosis , Bcl 2 , Bcl 10 , Bax , Bak , Bad , ICH 1 and CPP 32 , in Alzheimer ' s disease . ^^^ Apoptosis is regulated by the B cell leukemia 2 gene product ( Bcl 2 ) family ( Bcl 2 , Bcl 10 , Bax , Bak and Bad ) and the caspase family ( ICH 1 and CPP 32 ) , with apoptosis being prevented by Bcl 2 and Bcl 10 , and promoted by Bax , Bak , Bad , ICH 1 and CPP 32 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX is a proto oncogene of the BCL 2 family which can induce apoptosis . ^^^ Localization of BAX to the steroid secreting cells of the corpus luteum implies a functional role and BAX may interact with other members of the BCL 2 family to affect luteal function . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 has been demonstrated to inhibit apoptosis in breast cancer cells in vitro , and the ratio between Bcl 2 and its proapoptotic homologue Bax seems to be an important determinant of cellular sensitivity to induction of apoptosis . ^^^ Expression of the proapoptotic protein Bax correlated neither with Bcl 2 nor with the frequency of apoptotic cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the expression of p 53 , Bcl 2 and Bax in these cells was determined . ^^^ Western blotting performed on ventricular myocytes did not detect significant changes in p 53 , Bax and Bcl 2 proteins with age . ^^^ Similarly , immunocytochemically , the fraction of myocytes labeled by p 53 , Bax and Bcl 2 did not change from 3 to 24 months . ^^^ In conclusion , myocyte aging is characterized by an increase in diastolic calcium which may activate DNase 1 triggering apoptosis , independently from the expression of p 53 , Bax and Bcl 2 in the cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Among these , bcl 2 and bcl xL prevent cells from entering apoptosis , whereas bax and bcl xS can induce cell death . ^^^ Diminished susceptibility to chemotherapy has also been attributed , in in vitro systems , to alterations in the levels of bcl 2 , bax , or bcl 10 . ^^^ We analyzed the expression of bcl 2 , bax , bcl xL , and bcl xS in normal and neoplastic ovarian tissues by reverse transcriptase PCR and Western blotting . ^^^ Interestingly , the levels of these genes in normal and neoplastic tissues were significantly different : bcl 2 was higher in normal tissue ( P < 0 . 002 ) , whereas bax and bcl xL were higher in carcinoma ( P < 0 . 018 and P < 0 . 030 , respectively ) . bcl xS was present at low levels in 83 % of neoplastic samples and was undetectable in normal tissue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Elevated Bcl 2 / Bax are a consistent feature of apoptosis resistance in B cell chronic lymphocytic leukaemia and are correlated with in vivo chemoresistance . ^^^ We investigated the relationship between drug resistance and Bcl 2 / Bax in B cell chronic lymphocytic leukaemia ( B CLL ) . ^^^ Bcl 2 and Bax protein expression was quantified using FACS and a correlation between drug induced apoptosis and Bcl 2 / Bax was established . ^^^ Cells were then sorted into viable and nonviable populations according to their forward and side scatter characteristics and re analysed for Bcl 2 / Bax . ^^^ The most resistant cells had elevated Bcl 2 levels and low Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis is inversely related to bcl 2 but not to bax expression in salivary gland tumours . ^^^ AIMS : We investigated the extent of apoptosis in 55 benign and malignant salivary gland tumours and its association with the immunohistochemical expression of bcl 2 and bax . ^^^ METHODS AND RESULTS : Apoptosis was detected in histological sections by the 3 ' end DNA labelling method . bcl 2 and bax protein expression was determined by immunohistochemistry . ^^^ In contrast to bcl 2 , strong bax expression was found in both benign and malignant tumours and was not associated with the frequency of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A comparative examination of HVS transformed T cell clones and their native parental clones revealed that the expression of Bcl 2 , Bcl 10 ( L ) , Bax , and members of the tumor necrosis factor receptor ( TNF R ) superfamily with a death domain , namely , TNF RI , CD 95 , and TRAMP , were not modulated by HVS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Stretch mediated release of angiotensin 2 induces myocyte apoptosis by activating p 53 that enhances the local renin angiotensin system and decreases the Bcl 2 to Bax protein ratio in the cell . ^^^ Expression of angiotensinogen , AT 1 receptor , p 53 , and Bax increased and Bcl 2 decreased in stretched myocytes . ^^^ The changes in AT 1 receptor , p 53 , Bax , and Bcl 2 became more apparent with the duration of stretch . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Throughout the course of the disease , which peaked 12 14 days after inoculation and was followed by clinical recovery , we analyzed the DNA content of the spinal cord inflammatory cells to assess apoptosis and , simultaneously , we measured the expression of five proteins ( Fas , Fas ligand ( Fas L ) , Bcl 2 , Bcl 10 and Bax ) which modulate the apoptotic process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sensitive S 49 cells and resistant variants did not differ in the expression levels of the apoptosis regulating genes bax , bad , bcl 10 and bcl 2 , the status of the p 53 gene nor in a different requirement for the growth factors 2 2 , IL 4 or IL 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were neither immunopositive structures for bcl 2 or p 53 nor alteration of in situ expression of bcl xs / l or bax in any subject , and the TUNEL positive cells lacked immunopositivity against apoptosis related proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated apoptotic cell death in the cerebellum of five cases of XP group A ( XPA ) , four cases of CS , and twelve controls , using TdT mediated DIG dUTP nick end labeling ( TUNEL ) and immunohistochemical staining for bcl 2 , bcl 10 , p 53 , bax , BDNF and Trk B . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the nuclear localization of the transcription factor NF kappa B , the status of apoptosis and the expression of the Fas antigen , Fas ligand , Bcl 2 , Bcl xL and Bax by synovial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of TGF beta 1 and DEX on cellular amounts of several apoptosis related proteins , members of the Bcl 2 family , Bcl 2 , Bcl xL , Bcl xS , Bad , and Bax was also examined . ^^^ Bcl 2 and Bcl xS proteins were not detected , and Bax and Bad content did not change by treatment with TGF beta 1 or DEX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results suggest that the balance between cytokine and serum induced Bcl 2 expression and cytokine induced Bax expression may determine whether a cell undergoes cytokine induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition to Fas and FasL , we studied Bax , an intracellular protein of the apoptotic cascade , the Bax antagonist and anti apoptotic molecule Bcl 2 , and DNA fragmentation , the final step in the apoptotic pathway . ^^^ However , oligodendrocytes showed no Bax reactivity or DNA fragmentation and expressed high levels of Bcl 2 , as did the majority of infiltrating CD3+ cells , a pattern inconsistent with apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Importantly , the antiapoptotic factor Bcl 2 is sufficient to prevent p 53 mediated apoptosis and p 53 dependent transcription of Bax in ventricular myocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because genes of the Bcl 2 family influence longevity , we compared the expression of Bcl 2 , Bax , and A 1 among immature vs mature peripheral B cells using semiquantitative reverse transcriptase PCR . ^^^ While the levels of both Bcl 2 and Bax mRNA remain constant in these two populations , A 1 expression is strikingly up regulated among mature B cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl XL , and Bax are members of the Bcl 2 family that play important roles in apoptosis regulation . ^^^ However , we recently found by subcellular fractionation that whereas Bcl 2 is predominantly a membrane protein as previously reported , Bax and a significant fraction of Bcl XL are soluble in thymocyte and splenocyte extracts . ^^^ Induction of apo ptosis which causes the insertion of the soluble form of Bax into membranes did not result in appreciable Bax / Bcl XL , Bax / Bcl 2 or Bax / Bax dimer formation as determined by cross linking studies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of several apoptosis regulating proteins , including the Bcl 2 family proteins Bcl 2 , Bcl XL , Mcl 1 , Bax , Bak , and BAD ; the Bcl 2 binding protein BAG 1 ; and the cell death protease Caspase 3 ( CPP 32 ) , was evaluated by immunoblotting using 58 peripheral blood B CLL specimens from previously untreated patients . ^^^ Expression of Bcl 2 , Mcl 1 , BAG 1 , Bax , Bak , and Caspase 3 was commonly found in circulating B CLL cells , whereas the Bcl XL and BAD proteins were not present . ^^^ Apoptosis regulating proteins were not associated with patient age , sex , Rai stage , platelet count , hemoglobin ( Hb ) concentration , or lymph node involvement , although higher levels of Bcl 2 and a high Bcl 2 : Bax ratio were correlated with high numbers ( > 10 ( 5 ) / microL ) of white blood cells ( WBC ) ( P = . 01 ; . 007 ) and higher levels of Bak were weakly associated with loss of allelic heterozygosity at 13q14 ( P = . 04 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression in Alzheimer ' s disease . ^^^ In the present study , we have examined Bcl 2 and Bax immunoreactivity in the hippocampus of AD cases , with special attention to the possible relationship between Bcl 2 and Bax immunoreactivity , and neurofibrillary degeneration and senile plaques . ^^^ Different antibodies were used , including Bcl 2 ( N 19 ) , Bcl 2 ( BioGenex ) , Bax ( P 19 ) and Bax ( N 20 ) , and their specificity was tested on Western blots of brain homogenates . ^^^ No differences between Bcl 2 and Bax immunoreactivity in tangle bearing and non tangle bearing neurons were observed , thus suggesting that Bcl 2 and Bax do not participate in tangle formation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we have examined the expression of several proteins involved in the susceptibility to apoptosis in 20 human gliomas , including the BCL 2 family proteins BCL 2 , BCL 10 , BAX and MCL 1 , as well as p 53 and RB . ^^^ There was good correlation between expression of the functional antagonists , BCL 2 / BCL 10 and BAX , suggesting that changes in the BCL 2+BCL X / BAX ratio are not responsible for the differential response of glioma patients to chemotherapy . ^^^ There was no prominent association of outcome with the expression patterns of p 53 , RB , BCL 2 , BCL 10 or BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The identity of the insert of each clone was determined by slotblots of the DNA amplified from individual colonies and by hybridization with radioactive probes specific to the bcl 2 , bcl 10 , or bax genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This synthesis of wtp 53 protein induced by Adwtp 53 in A2780 / CP cells resulted in a significant increase in the expression of Bax protein without significantly effecting the expression of bcl 2 protein , and induced a dose dependent increase in the nucleosomal DNA fragmentation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 , proteins involved in regulation of cell survival , were studied by immunohistochemistry and Western blot analysis . ^^^ In addition , the expression of Bax , Bcl 2 , Bcl 10 ( L ) , and p 53 messenger RNA ( mRNA ) was analyzed by in situ hybridization . ^^^ Four days after termination of lactation , a peak in high molecular weight DNA fragments and an increase in Bax , along with a decrease in Bcl 2 proteins , were observed , in comparison with lactation and with 7 days post lactation . ^^^ The mRNAs for Bax and p 53 also were increased , whereas no changes were detected in Bcl 2 and Bcl 10 ( L ) mRNA levels . ^^^ In summary , these findings indicate that the cell remodeling of the anterior pituitary , after the termination of lactation , occurs through the process of apoptosis and involves changes in Bax , Bcl 2 , and p53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 are a pair of important genes that control programmed cell death , or apoptosis , with Bax being the apoptosis promoter and Bcl 2 the apoptosis protector . ^^^ In our preliminary study , we found that K 562 erythroleukemia cells have an extremely low level of endogenous Bcl 2 expression and a fairly high level of endogenous Bax expression . ^^^ We constructed Bax and Bcl 2 expression vectors and transfected them into K 562 cells . ^^^ Cell death analysis using the Annexin 5 assay showed that the Bax vector caused significantly more apoptotic cells that the Bcl 2 or pCI neo vector did . ^^^ After selection with G 418 , Bax , Bcl 2 and pCI neo stably transfected cells were established . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Positive ( ICE , ICErel 2 , ICErel 3 , Ich 1L , CPP 32 , mch 2 , mch 3 , bcl xS , bax and bak ) and negative ( bcl 2 , bcl xL , MCL 1 and Ich 1S ) regulators of apoptosis were successively examined using a semi quantitative technique of reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Baseline levels of message were detected for 3 ced 3 ( CPP 32 , Ich 1 and ICE ) and 4 ced 9 homologs ( bcl 10 , MCL 1 , bcl 2 and bax ) in the frontal cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Surface plasmon resonance was used to examine the kinetics of dimerization as a function of pH between the anti apoptotic protein Bcl XL ( applied in the mobile phase ) and three other members of the Bcl 2 family : Bcl 2 , Bax , and Bid ( immobilized on biosensor chips ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The position of a single intron was conserved in comparison to other members of the Bcl 2 family , namely Bax , CED 9 , Bcl 10 and Bcl 2 , but all other introns were displaced , consistent with a divergent phylogeny . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of two genes , BCL 2 , which inhibits apoptosis , and BAX , which induces apoptosis , was examined and correlated to the presence of risk factors that included hypertension , smoking , hyperlipidemia , and diabetes mellitus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Human glioblastoma cells were treated with or without TAM and / or IGF 1 in vitro and evaluated for : viability by the 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenol tetrazolium bromide cleavage assay ; apoptosis by histochemical analysis of nuclear morphology and 3 ' OH DNA fragments ; and expression of the IGF 1 receptor , and the bcl 2 , bcl xL , and bax proteins by immunoblot analysis . ^^^ Exogenous IGF 1 stimulated WITG 3 cell proliferation and significantly ( p < 0 . 05 ) antagonized the cytotoxic effects of TAM in a dose dependent fashion ; IGF 1 , but not TAM , enhanced expression of bcl 2 and bcl xL proteins ; however , bax protein expression was unchanged by either treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These cells were characterized by a lower level of bcl 2 protein than p 53 cells , whereas bax and OGAT expression was comparable in both lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TNFalpha plus NAC treatment resulted in a marked decrease in Bcl 2 protein levels in HIV 1 infected cells , coupled with an increase in Bax protein compared to uninfected cells , suggesting that the difference in susceptibility to TNFalpha induced apoptosis may relate to the differences in relative levels of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the presence of an increased number of apoptotic chondrocytes in TD fetuses was associated with a higher expression of Bax and the simultaneous decrease of Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In principle , p 53 may enhance chemosensitivity by promoting apoptosis via transcription independent mechanisms as well as transcriptional activation of proapoptotic genes such as bax and transcriptional repression of antiapoptotic genes such as bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , the expression of Bax and Bcl 2 , two genes associated with apoptotic cell death , was also measured following kindled seizures . ^^^ These results , coupled with our findings that the ratio of Bax / Bcl 2 expression is increased in the hippocampus by seizures , suggest that apoptosis of hippocampal interneurons may lead to dysinhibition in the hippocampus and increased seizure susceptibility . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the death related proteins ( DRPs ) Bcl 2 , Bax , Bcl 10 and Bak that regulate cell survival and death was examined using immuno histochemical methods in a group of 142 T 1 ( < 2 cm ) ductal breast carcinomas . ^^^ Expression of the apoptotic proteins Bax and Bak was present in 58 % of Bcl 2 negative tumors and associated significantly with an increase in apoptosis . ^^^ Expression of these DRPs was associated significantly with the HG of the tumors : Bcl 2 and Bak expression was predominant in HG I / II tumors , whereas expression of Bcl xL and Bax was commonly observed in HG 3 tumors , as occurs for p 53 over expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , the microtubule damaging drugs paclitaxel , vincristine , and vinblastine induced Bcl 2 hyperphosphorylation and apoptosis in MCF 7 and MDA MB 231 cells and reduced Bcl 2 Bax dimerization . ^^^ Paclitaxel or vincristine induced increased expression of Bax , while overexpression of Bcl 2 in these cell lines counteracted the effects of low doses of these drugs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no changes in levels of Bcl 2 , Bcl XL , or 24 kDa Bax over 72 hr after exposure to cisplatin or paclitaxel , but each agent led to up regulation of Bak and 21 kDa Bax in A 2780 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Semi quantitative RT PCR revealed that expression of c myc and bcl 2 genes was reduced during the apoptotic process , while expression of bax and bcl 10 ( L ) genes was not changed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a family member of the survival protein Bcl 2 , is expressed in the nervous system during development and throughout adulthood . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High grade malignant non Hodgkin ' s lymphomas differ from low grade lymphomas in the extent of apoptosis and their expression of bcl 2 , mcl 1 , bax and p 53 . ^^^ To study the influence of apoptosis regulating proteins bcl 2 , bax , mcl 1 and p 53 on the extent of apoptosis , we also immunostained the samples with antibodies to them . ^^^ In line with this difference , high grade lymphomas had significantly more cases with a weak expression of bcl 2 and strong expression of bax ( p = 0 . 00008 and p = 0 . 016 , respectively ) . ^^^ The results suggest that apoptosis affecting genes bcl 2 , bax , mcl 1 and p 53 all take part in the regulation of apoptosis in malignant non Hodgkin ' s lymphomas and contribute to a different level of apoptosis between high and low grade non Hodgkin ' s lymphomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the Bcl 2 family of apoptosis related genes ( bcl 2 , bcl 10 , mcl 1 , and bax ) and the proliferation and apoptosis related genes p 53 and cyclin D 1 were determined in 40 low T stage laryngeal carcinomas and in uvular epithelium from patients without SCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neural protective agents , propentofylline ( PPF ) could induce apoptotic cell death in the human glioma cells : analysis of Bcl 2 and Bax alpha / Bax beta expressions ] . ^^^ In the present study , we examined the apoptotic signal cascade , especially focusing on the expressing pattern of Bcl 2 / Bax gene products . ^^^ In the normal human astrocyte cells , Bax beta was markedly expressed , whereas Bcl 2 and Bax alpha proteins and mRNA were weakly or even nondetectable . ^^^ Accordingly , Bax beta might be a dominant variant in the normal glial cells , which could have the appropriate balance of proapoptotic ( Bax beta ) and anti apoptotic proteins ( Bcl 2 ) . ^^^ In the glioma cells , we showed the over expressions of Bcl 2 and Bax alpha compared with the normal counterparts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , higher concentrations of inostamycin induced morphological apoptosis and DNA fragmentation in Ms 1 cells without affecting the expression of p 53 , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression in neurofibrillary tangles in progressive supranuclear palsy . ^^^ Double labeling immunohistochemistry to Bcl 2 and Bax and to tau protein was examined in the brains of patients with progressive supranuclear palsy ( PSP ) to study the possible relationship between the expression of Bax and Bcl 2 proteins and neurofibrillary tangle formation . ^^^ No differences between Bcl 2 and Bax immunoreactivity in neurons of the brain stem were observed in PSP patients and controls . ^^^ Moreover , the intensity of Bcl 2 and Bax immunoreactivity was similar in tangle bearing and non tangle bearing neurons in PSP patients . ^^^ These results suggest that Bcl 2 and Bax are probably not implicated in neurofibrillary tangle formation in PSP . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , p21 / WAF / CIP , Bcl 2 , Bax , Bcl 10 , and Bak in radiation induced apoptosis in testicular germ cell tumor lines . ^^^ Expression of p21 / WAF / CIP was determined by Northern blot analysis and immunoblotting was used to monitor p 53 , Bax , Bcl 2 , Bcl 10 , and Bak protein levels . ^^^ Constitutive expression of Bax , Bcl 2 , Bcl 10 , and Bak was not affected by radiation and showed no correlation with cell susceptibility to radiation induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The S cells had undetectable levels of bax and high levels of bcl 2 , whereas the SN 3 cells had undetectable levels of bcl 2 levels and appreciable basal levels of bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
P 53 , bcl 2 , and Bax expression in renal cell carcinoma . ^^^ The loss of control of apoptosis may also contribute to progression and resistance to treatment modalities and can be attributed to an interaction between p 53 and the apoptotic regulators bcl 2 and Bax . ^^^ To determine whether the expression of p 53 , bcl 2 , or Bax could be correlated with outcome , we analyzed the expression pattern of these proteins in renal cell tumor samples . ^^^ Adjunctive use of bcl 2 and Bax staining currently plays a minimal role in helping to further stratify patients at high risk for disease progression or recurrence . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Zinc suppresses apoptosis of U 937 cells induced by hydrogen peroxide through an increase of the Bcl 2 / Bax ratio . ^^^ Hydrogen peroxide treatment for 1h with or without zinc increased both Bcl 2 and Bax proteins . ^^^ However , only Bax protein decreased to basal levels in the presence of zinc during the following 4h incubation , resulting in an increase of the Bcl 2 / Bax ratio and prevention of apoptosis . ^^^ These results indicate that the suppression of H2O2 induced apoptosis by zinc is mediated through an increase of the Bcl 2 / Bax ratio , which occurs upstream from the activation of CPP32 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The induction of apoptosis in MG 63 cells was associated with an increase in the ratio of the proapoptotic protein bax to the antiapoptotic protein bcl 2 , and oncostatin M prevented this change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To explain these findings , expression of Bcl 2 , Bax , c Myc and RB protein and mutations of the p 53 gene were analysed . ^^^ The ratio Bcl 2 / Bax was higher in U 1810 cells compared with U 1285 and U 1906 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some Bcl 2 related proteins ( Bcl 2 , Bcl xLong , MCl 1 , and Dad 1 ) function as cell death repressors , whereas others ( Bax , Bcl xShort , Bak , and Bad ) facilitate apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mitochondrial permeability transition ( PT ) pore , also called mitochondrial megachannel or multiple conductance channel , is a multiprotein complex formed at the contact site between the mitochondrial inner and outer membranes , exactly at the same localization at which Bax , Bcl 2 , and Bcl XL are particularly abundant . ^^^ Experiments involving the purified PT pore complex indicate that Bax , Bcl 2 , and Bcl XL exert at least part of their apoptosis regulatory function by facilitating ( Bax ) or inhibiting ( Bcl 2 , Bcl XL ) PT pore opening . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of apoptosis related gene expression by RT PCR showed that bcl 2 expression was greater in 4 pp R than in RVC . p 53 , bax and bcl xL were expressed at the same level in 4 pp R and RVC cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have reported that PPF induced apoptosis in human glioma cells and as its mechanism , up regulation of NGF , Fas , Bax beta and down regulation of Bcl 2 were assumed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine which proteins are involved in this apoptosis , we examined changes in protein levels of the Bcl 2 family , including Bcl 2 , Bcl 10 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Variable levels of bcl 2 , bcl 10 and bax mRNA in bladder cancer progression . ^^^ We investigated the expression of the anti apoptotic genes bcl 2 and bcl 10 and the pro apoptotic gene bax in bladder tumors and normal samples from urinary bladder , using RT PCR analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and c Myc , but not bax and p 53 , are expressed during human medullary thyroid tumorigenesis . ^^^ Eighteen MTCs from 18 patients ( including 12 sporadic and six familial cases and one metastatic lymph gland ) and a MTC cell line ( TT cells ) were used in this study for detecting the expression of apoptosis regulatory genes bcl 2 , bax , c myc , and p 53 . ^^^ Immunohistochemical results showed that all MTC tumor samples displayed Bcl 2 and c Myc immunoreactivity , whereas only 4 and 2 tumors showed a minority of cells positive for Bax and p 53 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in TGF beta 1 induced apoptosis of L 1210 leukemic cells . ^^^ In the present study , the involvement of proapoptotic ( bax ) and antiapoptotic ( bcl 2 ) genes in the molecular mechanism of TGF beta 1 induced apoptosis of L 1210 leukemic cells was investigated . ^^^ Bax transcript was measured using the RT PCR method with GAPDH as a `` housekeeping gene ' ' control , whereas Bcl 2 protein was determined using flow cytometry ( FITC conjugated monoclonal anti Bcl 2 antibody and FITC conjugated mouse anti IgG 1 antibody as a negative control ) . ^^^ The results of this study indicate that bax may function as a primary response gene and together with lowered Bcl 2 level may determine the induction of apoptotic process in L 1210 leukemic cells exposed to TGF beta1 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hyperthermia exerted no effect on the expression of Bcl 2 and Bax , regardless of the environmental acidity during heating . ^^^ It seemed that the increase in heat induced apoptosis in HL 60 cells in an acidic environment was due to a direct increase in the proteolytic cleavage of poly ( ADP ribose ) polymerase by acidic caspases without the involvement of Bcl 2 and Bax , and that heat induced apoptosis occurred during G 1 phase in HL 60 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The distribution of p21WAf1 / CiP1 , MDM 2 , and Bax / Bcl 2 proteins in ultraviolet ( UV ) irradiated and nonirradiated human skin was examined immunohistochemically and compared with p 53 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis , proliferation , bax , bcl 2 and p 53 status prior to and after preoperative radiochemotherapy for locally advanced rectal cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , Bcl 2 and Bax in cisplatin induced apoptosis in testicular germ cell tumour cell lines . ^^^ We examined the sensitivity for cisplatin induced apoptosis in a panel of four testicular germ cell tumour ( TGCT ) cell lines and monitored the cellular expression of the apoptosis related proteins p 53 , Bcl 2 and Bax . ^^^ Also endogenous levels of Bcl 2 and Bax expression did not correlate with cisplatin induced apoptosis . ^^^ In addition , the endogenous Bcl 2 and Bax expression was not affected by cisplatin treatment . ^^^ The present study suggests that , at least in our panel of TGCT cell lines , hypersensitivity for cisplatin induced apoptosis might not be necessarily correlated with the presence of wild type p 53 and is probably not associated with Bcl 2 and Bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The trophic action of IL 7 on pro T cells : inhibition of apoptosis of pro T 1 , T 2 , and T 3 cells correlates with Bcl 2 and Bax levels and is independent of Fas and p 53 pathways . ^^^ The IL 7 trophic affect correlated with increased intracellular levels of Bcl 2 and decreased levels of Bax , whereas no Bcl 10 ( L ) , Bcl w , or Bad was detectable . ^^^ Thus , maintaining a favorable Bcl 2 / Bax ratio may account for the trophic action of IL 7 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent studies have focused on the role that programmed cell death ( i . e . , apoptosis ) plays in both normal and neoplastic growth : certain genes can either suppress ( e . g . , Bcl 2 , Bcl xL ) or promote ( e . g . , Bik , Bax , Bak ) apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The fatty streaks differed from adaptive intimal thickenings by the presence of BAX ( P < 0 . 01 ) , a proapoptotic protein of the BCL 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mutational analysis of Bax and Bcl 2 in childhood acute lymphoblastic leukaemia . ^^^ In childhood acute lymphoblastic leukaemia there are large interpatient variations in levels of the apoptosis regulating proteins Bax and Bcl 2 , but the molecular basis for this variation is unknown . ^^^ DNA sequence analysis of the ORFs of bax and bcl 2 and of the uORF of bcl 2 revealed no mutations , despite the large range in expression levels . ^^^ Thus , mutations within the ( u ) ORFs of bax and bcl 2 that ( in ) activate or deregulate Bax and Bcl 2 are infrequent in primary childhood acute leukaemia and do not play a major role in regulation of the encoded proteins in this disease . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This regimen of melatonin followed by atRA induced cytocidal effects on MCF 7 cells by activating pathways leading to apoptosis ( programmed cell death ) as evidenced by decreased ER and Bcl 2 and increased Bax and transforming growth factor beta 1 ( TGF beta 1 ) expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Forced Gax expression led to Bcl 2 down regulation and Bax up regulation in mitogen activated , but not quiescent cultures . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the bcl 2 homologue bax in normal human skin , psoriasis vulgaris and non melanoma skin cancers . ^^^ Bax ( bcl 2 associated 10 protein ) is a recently identified member of the bcl 2 family and one of the principal inducers of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no statistically significant change in the expression of Bax , Bcl xl , Bcl 2 , or p 53 following androgen withdrawal . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax product , an inducer of apoptosis , and Bcl 2 protein , an inhibitor of apoptosis , were examined immunohistochemically in the seven autopsied renal tissues with amyloidosis and 10 autopsied control tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : Bax protein serves as a positive regulator of apoptosis by forming heterodimers with bcl 2 protein , thereby promoting cell survival . ^^^ METHODS : The authors assessed the relation between apoptosis and the expression of Bax and bcl 2 protein in fractionated radiation therapy for cervical carcinoma by using in situ nick end labeling ( ISEL ) and immunohistochemical methods . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of bcl 2 , bax , mcl 1 , and bcl 10 expression in ovarian surface epithelial tumors . ^^^ The expression of bcl 2 family proteins was investigated in 28 ovarian surface epithelial tumors , including serous and mucinous benign , borderline , and malignant tumors by immunohistochemical staining with antibodies to bcl 2 , bax , bcl 10 , and mcl 1 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In many tissues the apoptosis related gene products act in cohort : Bcl 2 and Bcl xl promoting survival of a cell , whilst Bax promotes cell death often positively regulated by the tumour suppressor gene p 53 . ^^^ Western blot analysis showed that : ( 1 ) Bcl 2 and p 53 were absent from interstitial Leydig cells but were expressed in the seminiferous tubules . ( 2 ) Bax protein although expressed in the interstitium was not present in the Leydig cells . ( 3 ) Bcl xl in Leydig cells was transiently increased after EDS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The drugs also induced an increase in p 53 related proteins , such as p 21 , Bax , and IGF binding protein 3 ( IGF BP 3 ) , and caused a significant reduction of the Bcl 2 level . ^^^ This event is accompanied by a decrease in the expression of Bcl 2 , a death antagonist , and an increase in that of Bax , a death agonist . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , BI 1 can interact with Bcl 2 and Bcl XL but Bax or Bak , as demonstrated by in vivo cross linking and coimmunoprecipitation studies . ^^^ BI 1 thus represents a new type of regulator of cell death pathways controlled by Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the mechanisms by which intracellular acidification leads to cell death , we have studied the roles of the antiapoptotic gene bcl 2 and its proapoptotic binding partner bax , the stress activated protein kinases ( SAPK / JNK ) , and the caspase proteases in mediating acid induced cell death . ^^^ Whereas the expression of bcl 2 in human bladder cancer MGH U 1 cells had no effect on acid induced death , overexpression of bax enhanced cell death , consistent with its proapoptotic function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As compared with parental cells and vector transfected clones , wtBRCA 1 clones exhibited : ( 1 ) a slightly decreased proliferation rate ( doubling time = 25 h as compared with 22 h for control cells ) ; ( 2 ) a ( 3 6 ) fold increase in sensitivity to chemotherapy drugs ( adriamycin , camptothecin , and taxol ) ; ( 3 ) increased susceptibility to drug induced apoptosis ; ( 4 ) reduced repair of single strand DNA strand breaks ; and ( 5 ) alterations in expression of key cellular regulatory proteins ( including BRCA 2 , p 300 , Mdm 2 , p 21 ( WAF1 / CIP1 ) , Bcl 2 and Bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We tested the role of bcl 2 by transfecting 2 low bcl 2 expressing myeloma cell lines , ARP 1 and 8226 , with a bcl 2 expression vector and compared the effects of DEX and MEL on apoptosis , cell cycle distribution and the levels of proapoptotic ( bax ) and antiapoptotic ( bcl 2 , bclx ) proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we have investigated by Western blotting the expression pattern of Bcl 2 and its homologues Bax , Bak , Bcl xL , Bcl xS , Mcl 1 and Bad in 12 distant lymph node metastases from patients who have been treated by different regimes , in nine newly established cell lines of these metastases , in three cell lines obtained from other sources and in primary melanocytic cell lines from three neonatal and two adult subjects . ^^^ Taken together , our data suggest that Bax , Bak , Bad , Bcl xL and Mcl 1 are expressed in addition to Bcl 2 in both normal melanocytes and in cell lines established from melanoma metastases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of the bcl 2 / bax pathway in hepatocyte apoptosis during acute rejection after rat liver transplantation . ^^^ The present study was designed to investigate the role of the bcl 2 / bax pathway in liver allograft tissue . ^^^ In group 1 , both bcl 2 mRNA and bax mRNA were expressed in trace amounts . ^^^ In group 2 , bcl 2 mRNA was slightly expressed while the expression of bax mRNA rose steadily . ^^^ In group 3 , bcl 2 mRNA expression levels remained similar to group 1 , while bax expression levels exceeded those in group 1 , but were less than in group 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 homologue mRNAs in rat liver allograft : rejection induced cell apoptosis is associated with upregulation of bax and bcl xs expression . ^^^ In this study , we screened for the expression of bcl 2 homologues ( bcl 2 , bax , bcl xl , and bcl xs ) and Fas ligand ( FasL ) by RT PCR method in grafts during acute rejection in rats following liver transplantation . ^^^ Both bax and bcl xs ( inducers of apoptosis ) mRNA levels increased steadily in the allografted group from postoperative day ( POD ) 2 to 8 , while no remarkable changes of bcl 2 and bcl xl expression ( inhibitors of apoptosis ) were recognized . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In particular , the content of the antiapoptotic products Bcl 2 and Bcl XL resulted to be increased in treated cells , whereas the expression of the proapoptotic protein Bax remained unaltered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , cells protected by BCL 2 demonstrated a block in this process in that BAX did not redistribute or homodimerize in response to a death signal . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine whether the altered sensitivity to this apoptotic signal was correlated with the expression of proteins of the bcl 2 family , the expression of bcl 2 , bcl 10 , and bax proteins was determined . ^^^ CD28+ and CD 28 CD4+ T cells could not be distinguished by the levels of bax or bcl xL protein ; however , CD4+CD28 T cells expressed higher amounts of bcl 2 protein than did CD4+CD28+ T cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : Bax protein , a member of the Bcl 2 family , regulates the apoptotic pathway that involves both Bcl 2 and p 53 . bax mutations are found in hematological malignancies , colon cancer with microsatellite mutator phenotype , and some other cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , and Waf 1 ) in precursor bronchial lesions of lung cancer . ^^^ Toward this aim , we analyzed , using immunohistochemistry , the expression of the p 53 gene and of its transcriptional target genes bax , bcl 2 , and waf 1 in preinvasive bronchial lesions from 69 patients with lung cancer and in similar lesions from 20 patients with no cancer progression . p 53 accumulation occurred with increasing frequency , from 19 % in mild dysplasia to 36 % in moderate dysplasia and 59 % in carcinoma in situ , and was exclusively observed in patients with p 53 positive carcinoma . ^^^ Bcl 2 overexpression and Bax down regulation , as shown by immunostaining , occurred in preinvasive lesions and were mainly maintained during invasion . ^^^ The expressions of bax , bcl 2 , and waf 1 did not correlate with p 53 status . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate the role of Bcl 2 family members in the inhibition of eosinophil apoptosis , we examined the expression of the known anti apoptotic genes Bcl 2 , Bcl xL , and A 1 , as well as Bax and Bcl xS , which promote apoptosis in other systems . ^^^ We show herein that freshly isolated human eosinophils express significant amounts of Bcl xL and Bax , but only little or no Bcl 2 , Bcl xS , or A 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Placement of the BCL 2 family member BAX in the death pathway of sympathetic neurons activated by trophic factor deprivation . ^^^ The BCL 2 family member BAX is required for the induction of apoptosis in neonatal sympathetic neurons after NGF withdrawal . ^^^ We find that neurons deficient in both Bax and Bcl 2 resist NGF deprivation similar to Bax deficient neurons discounting a role for BCL 2 in the mechanism by which Bax deficiency causes trophic factor independence . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of the bcl 2 apoptotic family of proteins in primary and recurrent cervical cancer . bcl 2 is one of a family of genes that control the apoptotic threshold of a cell . bcl 2 protein and its anti apoptotic homologue , mcl 1 , with the pro apoptotic protein , bax , are thought to function by forming homo and heterotypic dimers that then control the progression to apoptosis . p 53 is also involved as a down regulator of bcl 2 and a promoter of bax . ^^^ To determine the effect of these apoptotic mechanisms , we used immunohistochemistry to determine the prognostic significance of the expression of bcl 2 , mcl 1 , bax and p 53 in primary and recurrent cervical cancer . ^^^ Tissues from 46 patients with primary cervical cancer and 28 women with recurrent carcinoma were stained for bcl 2 , mcl 1 , bax and p 53 . ^^^ Positive bcl 2 staining defines a group of patients with primary disease with a good prognosis . p 53 , an activator of the bax promoter , identifies a group with a worse outcome . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related protein immunoreactivity defined presence and location of Bax , Fas , Fas Ligand ( FasL ) , Bcl 2 , interleukin 1 beta converting enzyme ( ICE ) , inducible nitric oxide synthase ( iNOS ) and the proto oncogene p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax are involved in the sympathetic protection of brown adipocytes from obesity linked apoptosis . ^^^ The brown adipocytes of obese rats have lower Bcl 2 / Bax mRNA and protein ratios than those of their lean littermates . ^^^ Exposure to a low temperature for three days markedly increased the Bcl 2 / Bax ratio , by increasing the noradrenergic output to BAT , which has previously been shown to reduce apoptotic cell death . ^^^ Micromolar NA concentrations increased the Bcl 2 / Bax mRNA and protein ratios , and protected against serum deprivation induced apoptosis . ^^^ We conclude that NA acts by modulating bcl 2 and bax gene expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined whether apoptosis is involved in hypoxic cell death using primary cultures of rat cortical neurons and whether the cell death is associated with changes in Bcl 2 and Bax expressions and activities of caspases . ^^^ This apoptotic process is accompanied by a rapid and sustained down regulation of Bcl 2 , whereas levels of Bax are unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resistance of pleural mesothelioma cell lines to apoptosis : relation to expression of Bcl 2 and Bax . ^^^ We asked whether mesothelioma was resistant to apoptosis and whether resistance was associated with altered expression of the antiapoptotic protein Bcl 2 or proapoptotic protein Bax . ^^^ By immunoblotting , 3 of 14 additional mesothelioma lines ( 9 of 14 with wild type p 53 ) expressed Bcl 2 but all 14 of 14 expressed the proapoptotic Bax , giving a low ratio of Bcl 2 to Bax . ^^^ We conclude that mesothelioma cell lines are resistant to apoptosis and that the failure in apoptosis is not explained by Bcl 2 but by other mechanisms that counteract the proapoptotic effect of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression in adult rat hearts after coronary occlusion : age associated differences . ^^^ Our results show that 1 ) DNA fragmentation began earlier and peaked earlier in the old compared with young adult hearts during infarction ; 2 ) there was heightened expression of both Bcl 2 and Bax in the old hearts at baseline ; and 3 ) the Bcl 2 to Bax ratio was higher in the older heart after coronary ligation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we report that the expression of bcl 2 , bcl xL , bcl xS , bax and bad mRNA as well as of Bcl 2 , Bax , Bcl XL , Bcl XS and Bag 1 proteins is not modulated in these two paradigms of neuronal cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , and Bcl 10 expression in the CA 1 area of the hippocampus following transient forebrain ischemia in the adult gerbil . ^^^ Immunohistochemistry and Western blotting to Bcl 2 , Bax , and Bcl 10 was examined in control ( age matched , non operated and sham operated ) and ischemic gerbils . ^^^ Bcl 2 immunoreactivity was low in CA 1 neurons , but Bax was highly expressed in CA 1 neurons of control gerbils . ^^^ Bcl 2 , Bax , and Bcl 10 were localized in dying cells , thus suggesting that expression of Bcl 2 was not sufficient to prevent nerve cells from dying . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In parallel , the quantitative reverse transcription polymerase chain reaction technique was used to determine the expression of bcl 2 , bax , p 53 , and glutathione peroxidase ( GSH Px ) genes . ^^^ After 72 hours , a decreased protein ratio of Bcl 2 to Bax was concomitant to evident loss of pericyte viability . ^^^ Their mRNA levels of bcl 2 , bax , and GSH Px essentially were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : Bax , a member of the Bcl 2 family of cell death regulators , induces cell death by promoting the induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative Western blotting was used to examine the protein expression of P 53 and P21WAF 1 , Bcl 2 and Bcl 10 ( L ) ( anti apoptotic proteins ) , and Bax , Bak , and Bad ( proapoptotic proteins ) . ^^^ Upon deprivation , these cancer cells up regulated P 21 and Bcl 2 and / or BclX ( L ) , lost response to withdrawal induced up regulation of Bax / Bad / Bak or decreased or even completely lost Bax expression and expressed some novel proteins such as P 25 and P54 / 55 complex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 expression , and apoptosis in gestational trophoblast of complete hydatidiform mole . ^^^ The sections were also immunostained with antibodies to p 53 , Bax and Bcl 2 proteins . ^^^ The fact that p 53 quantitative expression and an increase in the Bax / Bcl 2 ratio were also observed in CHM suggested that they may contribute partly to the high level of apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We used immunoblots to estimate levels of Bcl 2 , Bcl 10 ( l ) , and Bax at various times after carotid occlusion . ^^^ Therefore , Bax may disrupt the more favorable Bcl 10 ( l ) ( Bcl 2 ) interactions necessary for normal neuronal functioning and thus promote transient ischemic death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bcl xl , Bax , p 53 and poly ( ADP ) ribose polymerase ( PARP ) was demonstrated in tissue extracts by Western blotting . ^^^ However , in the long term treated testes , Bcl xl and PARP expression declined , Bax and p 53 protein concentrations were unchanged , and Bcl 2 was up regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunochemical analysis revealed that expression of apoptosis related gene products , such as Bcl 2 , Bcl xL and Bax , was below detectable levels with both cell types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 expression predict response to radiotherapy in human cervical cancer ] . ^^^ To establish a new predictor of the outcome of treatment for human cervical carcinoma , we investigated the relationship between the expression of the Bax and Bcl 2 proteins and the response to radiotherapy after administration of 10 . 8 Gy . ^^^ On univariate analysis , Bax and Bcl 2 protein expression prior to radiotherapy did not correlate with survival . ^^^ The levels of Bax and Bcl 2 expression after 10 . 8 Gy of radiotherapy are useful prognostic markers in patients with human cervical carcinoma . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have investigated by immunohistochemistry 38 cases of B cell MALT NHL comprising 23 high grade ( HG ) and 15 low grade ( LG ) tumours for the expression of p 53 , mdm 2 , p 21 , Rb , Ki 67 , bcl 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An exogenous cdk inhibitor , butyrolactone 1 , induces apoptosis with increased Bax / Bcl 2 ratio in p 53 mutated pancreatic cancer cells . ^^^ In butyrolactone 1 treated PANC 1 cells , expression of p 53 protein was unchanged , but Bax expression was slightly upregulated and Bcl 2 expression was predominantly down regulated . ^^^ Bax / Bcl 2 ratio reached 9 . 6 fold increase compared to the control at the maximum . ^^^ The time course of changes in Bax / Bcl 2 ratio was similar to that in the TUNEL positive ratio . ^^^ These data , suggest that dynamic changes of the Bax / Bcl 2 ratio might be important in determining point of apoptosis induction in pancreatic cancer cells with p 53 mutation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The bcl 2 gene was expressed in 54 % of the tumors ( 19 / 35 ) , whereas bcl 10 and bax gene products were present in only a low fraction of these lymphomas ( 4 / 35 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis demonstrated that both BaP and its 7 , 8 dihydrodiol metabolite affected a pathway involving Bcl 2 and Bax cytosolic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting of UV irradiated , differentiated keratinocytes did not support a role for either Bax or Bcl 2 in this process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At 14 days , CD 95 ligand and TNF alpha expression were reduced significantly , and the presence of 2 . 5 % ( range 1 . 5 3 . 75 % ) of apoptotic T cells at this time was probably due to cytokine deprivation , associated with decreased Bcl 2 relative to Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax protein expression in indolent versus aggressive B cell non Hodgkin ' s lymphomas . ^^^ Bcl 2 and bax are cellular proteins that are important in the regulation of apoptosis . ^^^ Overexpression of bcl 2 protein is associated with prolonged cell survival , whereas overexpression of bax correlates with increased apoptosis after injury . ^^^ It has been suggested that the ratio of bcl 2 and bax determines a cell ' s susceptibility to apoptosis . ^^^ We studied bcl 2 and bax expression by immunohistochemical methods in 46 cases of B cel non Hodgkin ' s lymphoma characterized by the Revised European American Lymphoma ( REAL ) classification to determine whether expression of these two proteins correlated with the histological subtype or the predicted clinical behavior ( indolent 5 aggressive ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the process of terminal differentiation toward mature neutrophils , the anti apoptotic proteins Bcl 2 and Bcl 10 become down regulated and eventually cease to be expressed , whereas the death promoting Bcl 2 homologue , Bax , persists . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A variety of parameters in these lines were analyzed to elucidate mechanisms of resistance including S phase , doubling time , expression and activity of topoisomerase 1 and 2 , expression of mdr 1 , p 53 status , cell cycle arrest , level of apoptosis , and expression of the apoptotic proteins Bcl 2 and Bax . ^^^ Increased apoptotic responses were generally observed in cell lines that were sensitive to etoposide and this correlated with low ratios of Bcl 2 / Bax protein . ^^^ However , the BT 474 line displayed a series of characteristics including slow growth , the presence of mutant p 53 , low topo 1 activity , and a high Bcl 2 / Bax ratio which together likely contributed to the resistance of this line to both etoposide and camptothecin . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 6 activation blocked anti IgM induced Bax ( alpha ) up regulation and , by doing so , corrected an imbalance in the Bcl 2 / Bax ratio that accompanies apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The roles of Bcl 2 protein and the protein ratio of Bcl 2 / Bax in regulating cell growth in various lymphoma cell lines were examined . ^^^ L bcl 2 did not induce growth inhibition in lymphoma cells not expressing Bcl 2 or Bax protein . ^^^ Thus , the protein ratio of Bcl 2 / Bax may also be important in regulating the growth of these lymphomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The amount of apoptosis was assessed by terminal deoxynucleotidyl transferase assay , and the expression of bcl 2 and bax was examined by immunohistochemistry . ^^^ A mosaic pattern of bcl 2 / bax positive myofibers characterized dystrophic muscles , thus the relative proportion of pro and antiapoptotic proteins differs among muscle fibers in correlation with the presence of apoptotic myonuclei . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family expression in salivary glands from patients with primary Sjgren ' s syndrome : involvement of Bax in salivary gland destruction . ^^^ To investigate the molecular mechanism of glandular parenchyma destruction in Sjgren ' s syndrome ( SS ) , Bcl 2 , Bax , Bcl 10 , and Bak expression were studied . ^^^ Both SS and control salivary gland ductal epithelial cells expressed Bcl 2 , Bax , and Bcl 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / Bax ratios in chronic lymphocytic leukaemia and their correlation with in vitro apoptosis and clinical resistance . ^^^ B lymphocyte Bcl 2 and Bax protein levels were found to be significantly altered in B CLL and increased Bcl 2 / Bax ratios were observed in both the treated and untreated patients compared with those of normal controls . ^^^ In order to determine whether Bcl 2 / Bax ratios affected cell survival via an anti apoptotic mechanism , cell death was induced in B CLL cells in vitro using chlorambucil , and apoptosis was monitored by Annexin 5 and propidium iodide staining . ^^^ Drug induced apoptosis in B CLL cells was inversely related to Bcl 2 / Bax ratios . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family proteins are key regulators of apoptosis and function as cell death antagonists ( e . g . , Bcl 2 , Bcl XL , and Mcl 1 ) or agonists ( e . g . , Bax , Bad , and Bak ) . ^^^ Here we report that among the Bcl 2 family of proteins tested ( Bcl 2 , Bcl XL , Mcl 1 , Bax , Bad , and Bak ) , Bcl XL was unique in that its protein levels were tightly regulated by hemopoietins in both immortal and primary myeloid progenitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We used immunoprecipitation experiments to ask if bcl 2 phosphorylated after ATRA or taxol had altered capacity to dimerize with bax . ^^^ We conclude that : bcl 2 is phosphorylated on serine after treatment of AML blasts with ATRA ; bcl 2 phosphorylation after ATRA is different from that seen after taxol ; bcl 2 phosphorylated after either agent retains capacity to dimerize with bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , we assessed the expression of the cytoplasmic proteins Bax and Bcl 2 , respectively a promoter and an inhibitor of apoptosis , and the expression of cyclin A , a nuclear protein that induces proliferation of smooth muscle cells . ^^^ Compared with WKY , cells from SHR exhibited normal Bax expression , increased ( P < 0 . 001 ) Bcl 2 expression and increased ( P < 0 . 001 ) cyclin A expression . ^^^ The ratio of Bax to Bcl 2 , an index of cell susceptibility to apoptosis , was lower ( P < 0 . 001 ) in SHR than in WKY . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the expression of Bax , Bcl 2 , Bcl 10 , and Mcl 1 in human benign nevi , primary MM , and metastatic MM using immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To gain insight into the regulation of cardiac apoptosis in arterial hypertension , we investigated the expression of the proteins Bcl 2 ( an inhibitor of apoptosis ) and Bax ( an inducer of apoptosis ) in the left ventricle of 30 week old normotensive Wistar Kyoto rats ( WKY ) , SHR , and SHR treated with the angiotensin 2 type 1 receptor ( AT 1 ) antagonist losartan ( 20 mg 10 kg ( 1 ) 10 d ( 1 ) ) during 14 weeks before death . ^^^ The expression of Bcl 2 and Bax was assessed by Western blot analysis . ^^^ Compared with WKY , untreated SHR exhibited increased ( P < 0 . 05 ) apoptosis , increased ( P < 0 . 01 ) Bax , and similar Bcl 2 . ^^^ The Bcl 2 / Bax ratio ( an inverse index of cell susceptibility to apoptosis ) was lower ( P < 0 . 05 ) in untreated SHR than in WKY . ^^^ The chronic administration of losartan was associated with the normalization of apoptosis , Bax expression , and the Bcl 2 / Bax ratio in treated SHR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Formalin fixed tissue sections were immunohistochemically stained for apoptosis associated proteins ( Bcl 2 , Bcl 10 , Bax , Bak , p 53 , MDM 2 , BHRF ) . ^^^ The irregular expression of Bcl 2 , Bcl 10 , Bax , and Bak in oral lymphomas indicates dysfunctional apoptotic mechanisms in these tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis associated regulatory proteins were also affected by cell infection : expression of Bcl 2 was decreased in the infected cells whereas expression of Bax become more variable , with some cells showing higher levels of this protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Peptide mass fingerprinting identified this protein as Bid , a BH 3 domain containing protein known to interact with both Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX , a heterodimer partner of BCL 2 , is an apoptosis inducer . ^^^ The monoclonal antibody anti BAX 4F11 was used on paraffin sections : immunodetection of BCL 2 was performed simultaneously on serial sections . ^^^ There was no strict correlation between the IHC profiles of BAX and BCL 2 expression , although a reciprocal pattern of staining was observed in lymph node and colon . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , the bcl 2 related proteins ( bax , bad , and bak ) act by promoting apoptotic cell death as shown from their expression in hematopoietic cell lines . ^^^ Similarly , we analyzed the expression of bcl 2 related proteins bcl xL , bax , bad , and bak before and during ex vivo expansion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the yeast cell assay , BOD interacts with diverse antiapoptotic Bcl 2 proteins [ Mcl 1 , Bcl 2 , Bcl xL , Bcl w , Bfl 1 , and Epstein Barr virus ( EBV ) BHRF 1 ] but not with different proapoptotic Bcl 2 proteins ( BAD , Bak , Bok , and Bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High micromolar concentrations of topotecan , which are unlikely to be achieved in plasma in human patients in vivo , were cytotoxic within 48 hr , induced DNA fragmentation , did not induce major cell cycle changes , failed to consistently alter BCL 2 or BAX protein levels but inhibited RNA synthesis and induced cleavable DNA / topoisomerase 1 complex formation . ^^^ Prolonged exposure for 72 hr to high nanomolar to low micromolar concentrations of topotecan augmented p 21 protein levels and induced G2 / M arrest but failed to consistently alter BCL 2 and BAX protein levels , did not induce significant DNA / topoisomerase 1 complex formation and did not inhibit RNA synthesis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We did not observe any changes in Bcl 2 or Bcl 2 related proteins ( Bcl 10 , Bax , and Bad ) in control or KCREB transfected cells before or after treatment with Tg . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study apoptotic signaling pathways in cardiomyocytes and to understand IGF 1 actions on the apoptotic signaling of cardiac muscle cells , we have defined the effects of IGF 1 on Bcl 2 , Bax , caspase 3 , DNA fragmentation , and cell survival in primary cardiomyocytes . ^^^ Compared with Bax levels , the levels of Bcl 2 were found to be quite low in these cells . ^^^ The levels of Bcl 2 associated Bax were increased after serum withdrawal and incubation with doxorubicin and were reduced by IGF 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The presence and distribution of apoptotic cell death in multiple system atrophy ( MSA ) and morphologically related diseases were investigated by means of a modified terminal deoxynucleotidyl transferase mediated nick end labeling method , comparing their distribution with that of glial cytoplasmic inclusions , immunohistochemically demonstrated bcl 2 protein , bax protein , CD 95 , TNFalpha , and p 53 protein expression , as well as activated microglia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate molecular events in the apoptosis , expressions of Bcl 2 protein family members , such as Bcl 2 , Bcl 10 and Bax , and heat shock protein 70 ( HSP 70 ) were measured by western blotting using specific antibodies . ^^^ The levels of Bax and Bcl Xs remained largely unchanged , but the Bcl 2 and Bcl XL expression showed down regulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our BAX deletion constructs and minimal domain constructs indicated that the BH 3 domain was required for BAX homodimerization and heterodimerization with BCL 2 , BCL XL , and MCL 1 . ^^^ This suggests that BAX ' s killing function reflects mechanisms beyond its binding to BCL 2 or BCL XL to inhibit them or simply displace other protein partners . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In 103 evaluable patients we performed a multivariate analysis of the tumour biological factors , i . e . histological grade , oestrogen receptor ( ER ) , progesterone receptor ( PR ) , S phase fraction ( SPF ) , ploidy , p 53 , c erbB 2 , Bcl 2 and Bax expression , which showed significance in the univariate analysis according to treatment response , time to progression ( TTP ) or overall survival ( OS ) . ^^^ In the univariate analysis grade , PR , Bax and Bcl 2 correlated significantly with TTP , whereas in the multivariate analysis only PR showed a statistically significant correlation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It is , therefore , important to clarify if the Bax protein is limiting for activation of the genetic program of programmed cell death or can be complemented by different Bcl 2 family members , such as Bak or Bad . ^^^ Instead , the survival rate of terminally differentiated Bax inactivated HL 60 ( Bax ( ) HL 60 ) cells is almost three times higher in respect to control cultures , indicating that in mature granulocytes Bax is not efficiently complemented by others members of the Bcl 2 family proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The action of Bax and bcl 2 on T cell selection . ^^^ We have studied the effect of bax and bcl 2 transgenes in recombination activation gene 1 deficient ( RAG 1 ( / ) ) mice transgenic for the major histocompatibility complex class 1 restricted F 5 T cell receptor . ^^^ Overexpression of a bax transgene in the thymus seriously impairs the production of mature T cells , whereas bcl 2 overexpression greatly promotes it . ^^^ The effect of bax and bcl 2 overexpression on antigen induced negative selection was studied using fetal thymic organ cultures . ^^^ This analysis showed that Bcl 2 strongly inhibits negative selection , whereas Bax does not affect it . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bleomycin induced apoptosis was accompanied by decreases in bcl 2 and bcl xl and increases in p 53 , bak , and bax protein levels . ^^^ Phenobarbital inhibited both TGF beta and bleomycin induced apoptosis and the normal regulation of p 53 , bcl 2 , and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further analysis to determine the neurotoxic mechanism revealed decreased Bcl 2 and increased Bax expression together with caspase 3 activation in cortical neurons cocultured with NO producing astrocytes . ^^^ Inhibition of NO production in astrocytes by N ( G ) monomethyl L arginine , an inhibitor of NOS , significantly inhibited neuronal death together with changes in Bcl 2 and Bax protein levels and in caspase 3 like activity . ^^^ These data suggest that NO produced by astrocytes after hypoxic insult induces apoptotic death of neurons through mechanisms involving the caspase 3 activation after down regulation of Bcl 2 and up regulation of Bax protein levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot quantification of Bax and Bcl 2 ( pro and antiapoptotic gene products , respectively ) showed higher Bax and lower Bcl 2 expression , and accordingly increased the Bax to Bcl 2 ratio in aorta from SHR treated with enalapril or amlodipine in comparison to untreated SHR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax and Bcl 10 expression in neuronal apoptosis : a study of mutant weaver and lurcher mice . ^^^ We investigated the expression of the apoptosis modulating proteins Bcl 2 , Bax and Bcl 10 in the cerebellum of mutant lurcher and weaver mice . ^^^ The observed expression patterns of Bcl 2 , Bax and Bcl 10 protein in apoptotic lurcher and weaver neurons support the hypothesis that the execution of neuronal apoptosis involves increased expression of Bax , which could represent a general mechanism in diverse neurodegenerative processes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protective effects of KGF were independent of alterations in catalase , glutathione ( GSH ) , or the expression of bcl 2 and bax , two protooncogenes known to regulate oxidant induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We analysed transcription of two genes that regulate apoptosis , bcl 2 and bax , and found that both are transcribed in mouse and human preimplantation embryos . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 2 and IL 15 act , at least in part , repressing 197 / 15a transcription ; their effect on 197 / 15a mRNA accumulation is partially independent of novel protein synthesis , likely not mediated by JunB , Bcl 2 , or Bax , and requires the activity of rapamycin sensitive molecule ( s ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Strong induction of BAX expression and loss of BCL 2 expression , which are associated with p 53 dependent apoptosis rather than growth arrest , were not observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Incubation with the IFNs increased the Fas antigen expression in one of three cell lines but did not alter the expression of Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The activation of caspase 3 by IR may thus engage at least two separate mechanisms , one through the regulation of the bcl 2 family members by p 53 , whereas the other yet to be identified one involves neither p 53 nor bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the mechanisms by which intracellular acidification leads to cell death , we have studied the roles of the anti apoptotic gene bcl 2 and its pro apoptotic binding partner bax , the Stress Activated Protein Kinases ( SAPK / JNK ) , and the caspase proteases in mediating acid induced cell death . ^^^ While expression of bcl 2 in human bladder cancer MGH U 1 cells had no effect on acid induced death , overexpression of bax enhanced cell death , consistent with its pro apoptotic function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 expression is confined to the base of the colonic crypt , whereas transforming growth factor beta ( TGFbeta ) is expressed in the upper crypt , as are the apoptotic death promoters , Bak and Bax . ^^^ There was no change in the levels of the p 30 phosphorylated form of Bcl 2 or in levels of the proapoptotic proteins Bax or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A dose and time response study revealed that 5 nmol of MK886 / 10 ( 6 ) cells was sufficient to induce apoptosis both in control and bcl xL cells , respectively , but to different degrees . bcl xL and bcl 2 proteins , but not bax or FLAP , were decreased by 4 h after 5 nmol of MK886 / 10 ( 6 ) cells in both cell lines , although the higher levels of bcl xL in overexpressors took longer to disappear . ^^^ This early loss of bcl xL and bcl 2 was not attributable to generalized proteolysis , as shown by Coomassie Blue staining and by the maintenance of bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Predictive value of Bcl 2 and Bax protein expression for chemotherapeutic effect in gastric cancer . ^^^ We investigated tissue staining for Bcl 2 and Bax proteins , which regulate apoptosis , as indicators of chemotherapeutic effect in patients with gastric cancer . ^^^ In 23 patients with gastric carcinoma biopsy specimens were obtained endoscopically prior to chemotherapy and stained immunohistochemically with anti Bcl 2 and anti Bax antibodies . ^^^ No correlation was seen between chemotherapeutic effect and Bcl 2 or Bax alone . ^^^ However , among the Bax positive cases , the patients with Bcl 2 positive tumors were significantly more chemoresistant ( p = 0 . 036 ) and had worse prognoses ( p = 0 . 008 ) than Bcl 2 negative cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 was downregulated by both agents based on Northern and Western analysis , while the level of Bax expression remained unchanged compared to control cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the relationships among several factors related to proliferation and apoptosis in LAM cells , we employed immunohistochemical methods for the localization of Bcl 2 and Mcl 1 ( inhibitors of apoptosis ) , Bax ( a promoter of apoptosis ) , c Myc ( an apoptosis related oncoprotein ) , proliferating cell nuclear antigen ( an indicator of mitotic activity ) , and nick end labeling ( to identify apoptotic cells ) in lung tissues of 9 patients with LAM . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase in paclitaxel or etoposide induced apoptosis of HL 60 / Apaf 1 cells was not associated with any significant alterations in Bcl 2 , Bcl xL , Bax , Fas , or Fas ligand expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 ( L ) , Bax , Bad , Bak and p 53 protein expression was analysed by Western blotting . ^^^ LoVo ) , 5 FU induced apoptosis was accompanied by increased expression of Bax and Bak without consistent modulation of other bcl 2 family proteins . ^^^ There was a significant correlation between chemosensitivity and Bcl 10 ( L ) to Bax ratio , rather than Bcl 2 to Bax . ^^^ In conclusion , these results suggest that some members of the Bcl 2 family of proteins , in human colon cancer cell lines , are modulated by 5 FU and that the ratio of Bcl 10 ( L ) to Bax may be related to chemosensitivity to 5 FU . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proteins involved in cell death ( bax ) or cell survival ( bcl 2 ) are present in oocytes as well as compounds ( TNF alpha , Fas ) involved in the initiation of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is an important determinant of chemosensitivity in pediatric tumor cell lines independent of Bcl 2 expression and p 53 status . ^^^ The proteins evaluated were p 53 and six members of the Bax / Bcl 2 family : three proapoptotic proteins ( Bax , Bak , and Bcl xS ) and three survival factors ( Bcl 2 , Bcl xL , and Mcl 1 ) . ^^^ We have demonstrated that endogenous Bax expression was the only protein found to be associated with chemosensitivity across the three different tumor histiotypes and propose that analysis of Bax may be a more useful prognostic indicator for tumor response to therapy than either p 53 or Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study addressed the hypothesis that the neuronal mineralocorticoid receptor ( MR ) regulates genes associated with cell death , such as bax and p 53 , and cell viability , including bcl 2 , BDNF , and NT 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of bax protein in sphingosine induced apoptosis in the human leukemic cell line TF 1 and its bcl 2 transfectants . ^^^ Immunoprecipitation with anti bcl 2 antibody , followed by immunoblotting with anti bax antibody , showed that both the 21 kDa bax protein and the 18 kDa protein heterodimerized with bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 expressions predict response to radiotherapy in human cervical cancer . ^^^ PURPOSE : The ratio of Bcl 2 to Bax expression determines survival or death following an apoptotic stimulus . ^^^ In order to establish a new predictor of the outcome of treatment for human cervical carcinoma , we investigated the relationship between the expressions of the Bax and Bcl 2 proteins and the response to radiotherapy after the administration of 10 . 8 Gy . ^^^ The Bax and Bcl 2 protein expressions prior to radiotherapy did not correlate with response and survival . ^^^ However , the Bax and Bcl 2 protein expressions after radiotherapy correlated with both response and survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , we have found that several early paclitaxel mediated events are enhanced in SW 626 transfectants that overexpress BAX , including G 2 M phase arrest , tubulin polymerization , and BCL 2 phosphorylation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the Bcl 2 , Mcl 1 , Bcl 10 , Bax , and Bak proteins was analyzed to determine whether the differences in MCF 7 cell sensitivity to apoptosis could be correlated to the differential expression of these proteins . ^^^ Whereas Bak , Bcl 10 , and Mcl 1 levels were identical between variants , the levels of Bcl 2 were 3 . 5 3 . 8 fold higher and the levels of Bax were 1 . 5 1 . 7 fold lower in the resistant variants ( M and L ) as compared with those of the sensitive variant ( N ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We explored whether the putative channel forming fifth and sixth alpha helices of Bcl 2 and Bax account for Bcl 2 mediated cell survival and Bax induced cell death in mammalian cells and in the yeast Saccharomyces cerevisiae . ^^^ When alpha 5 alpha6 were either deleted or swapped with each other , the Bcl 2Deltaalpha5alpha6 deletion mutant and Bcl 2 Bax ( alpha5alpha6 ) chimeric protein failed to block apoptosis induced by either Bax or staurosporine in human cells and were unable to prevent Bax induced cell death in yeast , implying that the alpha 5 alpha6 region of Bcl 2 is essential for its cytoprotective function . ^^^ BaxDeltaalpha5alpha6 and Bax Bcl 2 ( alpha5alpha6 ) also retained the ability to dimerize with Bcl 2 . ^^^ Bax therefore may have redundant mechanisms for inducing apoptosis in mammalian cells , based on its ability to form alpha 5 alpha6 dependent channels in membranes and to dimerize with and antagonize anti apoptotic proteins such as Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have previously examined the involvement of the B cell leukemia 2 gene product ( Bcl 2 ) family proteins ( Bcl 2 , Bcl 10 , Bax , Bak , and Bad ) in Alzheimer ' s disease ( AD ) and found that Bcl 2 , Bcl 10 , Bak , and Bad were upregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR shows that in REHIPs , Abeta decreases mRNA expression of Bcl 2 , as well as the ratio of Bcl xL / Bcl xS , with little effect on Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 relative to Bax decreased more after combined treatment with cisplatin and ATRA than after either drug alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of LNCaP cells with 10 nm Taxol led , after 24 h , to relatively specific and almost total down regulation of bcl xL protein in the absence of alteration of bax , bak , or bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We sought to examine the role of p 53 gene function and alterations in cell cycle progression or other mechanisms of action of UCN 01 including perturbation of the apoptosis pathway mediated by NF kappaB and Bcl 2 / Bax . ^^^ Bcl 2 and Bax levels were determined by Western immunoblotting . ^^^ UCN 01 does not effect NF kappaB DNA binding activity or Bcl 2 and Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax were minimally detectable at baseline ; infection with Ad5CMV p 53 induced no changes in the protein levels in the NPC cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For example , the androgen independent highly metastatic Dunning AT 3 subline , while expressing bax protein , does not express bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Many genes are involved in apoptosis modulation , including p 53 , bcl 2 and bax . ^^^ We determined the roles of p 53 status and the ratio of bcl 2 / bax proteins for predicting the recurrence of bladder superficial transitional cell carcinoma that had been treated with intravesical chemotherapy after resection . ^^^ MATERIALS AND METHODS : We performed Western blot analysis to express bcl 2 and bax proteins , and PCR SSCP to determine the alteration in the p 53 gene in 43 patients with transitional cell carcinoma of the bladder treated with intravesical chemotherapy after tumor resection . ^^^ RESULTS : The expression of bcl 2 or bax did not correlate with histological grade , clinical stage or relapse . ^^^ In cases of relapse within 1 year after resection bcl 2 / bax was greater than and less than 1 in 50 and 11 % , respectively ( p < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that treatment of cells with vinblastine induced phosphorylation of Bcl 2 , resulting in the dissociation of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of Bcl 2 , Bax , and Bcl XL were not changed following the co treatment with inostamycin plus paclitaxel , whereas the activated form of caspase 3 was markedly increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Potentiation of 2 chlorodeoxyadenosine activity by bryostatin 1 in the resistant chronic lymphocytic leukemia cell line ( WSU CLL ) : association with increased ratios of dCK / 5 ' NT and Bax / Bcl 2 . ^^^ Results from Western blot analysis indicate that WSU CLL cells express high levels of Bcl 2 , Bcl xL and c myc , and a low level of Bax . p 53 in untreated WSU CLL cells is undetectable . ^^^ WSU CLL cells treated with bryostatin 1 showed a significant increase in the ratio of Bax to Bcl 2 . ^^^ The bryostatin 1 induced increased ratio of dCK / 5 ' NT activity and an increased ratio of Bax / Bcl 2 are at least two mechanisms through which this natural compound is able to potentiate the anti tumor activity of 2 CdA in otherwise resistant CLL cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We treated human brain aggregate cultures with these cytokines and determined their effect on the mRNA and protein levels for Bcl 2 , Bcl ( 10 ) and Bax alpha . ^^^ Following TNFalpha treatment , Bcl 2 protein is significantly decreased , and at least one additional Bax isomer emerges . ^^^ We conclude that Bcl 2 and Bax alpha proteins play significant roles in modulating neural cell death from TNFalpha but not from PAF induced cell damage . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Spontaneous expression of bcl 2 , bax , c myc . c fos , c jun , p 53 , Fas and tumour necrosis factor ( TNF ) alpha by bone marrow cells was measured using either semiquantitative or quantitative reverse transcription polymerase chain reaction in SLE patients ( n = 8 ) and in eight normal control subjects . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax was clearly induced only on IL 2 stimulated or PMA plus ionomycin stimulated PBLs and that of other Bcl 2 family proteins such as Bcl 10 and Bad could not be detected on human PBLs , including IL 2 stimulated or PMA plus ionomycin stimulated PBLs . ^^^ The resistance of these activated PBLs to Fas mediated apoptosis may be due to the augmented Bcl 2 expression or the presence of Bcl 2 : Bax heterodimers on these cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No overall significant change in bcl 2 or bax expression on G0 / G1 cells was found after 3 days ' suspension culture with the analogues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Members of the Bcl 2 family of proteins , Bcl 2 , Bcl 10 ( L ) , Bcl Xs and Bax , are considered to play important roles in the regulation of apoptosis and drug resistance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of cyclin D 1 , bcl 2 , and bax proteins , proliferating cell nuclear antigen ( PCNA ) , and DNA ploidy in squamous cell carcinoma of the oral cavity . ^^^ The prognostic role of the expression of bcl 1 , bcl 2 , bax , PCNA , and DNA ploidy in a series of 25 oral squamous cell carcinoma ( SCC ) was investigated . ^^^ Immunohistochemistry for PCNA , bcl 2 , bcl 1 , and bax proteins was carried out on 5 microm serial sections from formalin fixed , paraffin embedded tissue . ^^^ All of our cases of SCC showed the presence of aneuploid populations , which was not correlated with the clinicopathologic parameters or with the overexpression of bcl 1 , bcl 2 , bax , and PCNA . ^^^ Nevertheless , once the parameters considered for the evaluation of DNA were examined in detail , it appeared that some of them , individually or combined with each other or with the expression of bcl 1 , bcl 2 , and bax , gained statistical significance in predicting the clinical evolution of SCC of our series . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of the genes associated with apoptosis , dose dependent reduction of bax expression was demonstrated in KLE cells , while induction of WAF 1 was seen in Hec 1A and RL 95 2 cells , and reduction of bcl 2 was demonstrated in RL 95 2 cells . ^^^ The mechanism of inhibition involves apoptosis , and regulation of bax , bcl 2 , and WAF 1 is demonstrated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis correlated with an increase in BAX protein , an established inducer of programmed cell death , and an increase in the ratio of BAX to BCL 2 , an established inhibitor of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 but not Bax expression is associated with apoptosis in normal and transformed squamous epithelium . ^^^ Relative levels of apoptosis regulatory proteins , such as Bcl 2 and Bax as well as interactions of these proteins with other gene products , may contribute to the rate of apoptosis in neoplasia . ^^^ Bcl 2 protein expression was associated with poorly differentiated tumor grade but was not correlated with Bax expression or patient survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , and p 53 in high grade prostatic intraepithelial neoplasia and localized prostate cancer : relationship with apoptosis and proliferation . ^^^ The aim of this study was to examine and correlate the expression of the apoptosis regulating genes bcl 2 , bax , and p 53 with the frequency of apoptosis and rate of proliferation in benign prostatic epithelium ( BP ) , prostate cancer , and high grade prostatic intraepithelial neoplasia ( HGPIN ) , which is currently considered the most likely precursor of prostate cancer . ^^^ Immunohistochemistry was performed to assess expression of bcl 2 , bax , and p 53 , and proliferation rate , as measured by the Ki 67 index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of c jun N terminal kinase / stress activated protein kinase and the decreased ratio of Bcl 2 to Bax are associated with the auto oxidized dopamine induced apoptosis in PC 12 cells . ^^^ Meanwhile , Bcl 2 started to decrease after onset of apoptosis , and Bax was increased up to beginning of apoptosis , and thereafter decreased . ^^^ Therefore , these results suggested that activation of JNK / SAPK and the decreased ratio of antiapoptotic Bcl 2 to proapoptotic Bax appear to be associated with the dopamine induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because inherent cellular radioresistance plays a critical role in the failure of radiotherapy , in this study , we investigated whether there is a correlation between the ratio of two apoptosis regulators , bcl 2 ( apoptosis suppressor ) and bax ( apoptosis inducer ) in prostatic tumors and the clinical response to radiotherapy in patients with localized prostate cancer . ^^^ Immunohistochemical analysis was performed on paraffin embedded prostate sections to determine the level of expression of the two apoptotic proteins , bcl 2 and bax , in tumor cells . ^^^ The resulting significantly higher bcl 2 / bax ratio ( P < 0 . 01 ) correlated with poor therapeutic responsiveness of prostate cancer to radiotherapy ( 1 . 12+ / 0 . 12 and 0 . 56+ / 0 . 13 , for nonresponders and responders , respectively ) . ^^^ CONCLUSIONS : These findings suggest that patients with an elevated bcl 2 / bax ratio are at increased risk of their cancer failing to respond to radiotherapy . ^^^ This study suggests a predictive value for the bcl 2 / bax ratio as a potential molecular marker for predicting radioresistance of prostatic tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic index and apoptosis influencing proteins bcl 2 , mcl 1 , bax and caspases 3 , 6 and 8 in pancreatic carcinoma . ^^^ AIMS : To study the expression of bcl 2 , bax and mcl 1 and caspases 3 , 6 and 8 and apoptosis in pancreatic carcinoma . ^^^ METHODS AND RESULTS : Eighty seven pancreatic carcinomas were studied immunohistochemically with antibodies to bcl 2 , mcl 1 , bax and caspases 3 , 6 and 8 . ^^^ Apoptosis was detected by the TUNEL method . bcl 2 and mcl 1 positivity was observed in 13 % and 86 % of the cases , while bax was observed in all of them . ^^^ CONCLUSIONS : Both bcl 2 and bax influence the extent of apoptosis in pancreatic carcinoma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The predictive role in terms of pathological response and prognostic role of biomarkers such as GST pi , p 53 , bcl 2 and bax expression , immuno histochemically detected , and of the S phase cell fraction , autoradiographically determined as thymidine labeling index ( TLI ) , were investigated within a prospective randomized phase 3 clinical trial on squamous cell carcinoma of the oral cavity , including surgery or primary chemotherapy ( PCT ) , which foresaw the prospective determination of biological markers . ^^^ All biomarkers were unrelated , except for an inverse relation between TLI and GST pi and a direct relation between bcl 2 and bax expression . ^^^ Conversely , low GST pi and bax expression were indicative of pathological response but lost relevance as predictors of DFS , whereas absence of bcl 2 was associated with high probability of 3 year DFS in the overall series as well as in non responding patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neither wild type p 53 status , nor p 53 protein accumulation , nor p 21 or MDM 2 levels , nor differential expression of BCL 2 family proteins predicted drug sensitivity , except for an association of BAX with higher beta lapachone sensitivity in acute cytotoxicity assays . p 16 protein expression was associated with high doubling time and chemoresistance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using isolated mitochondria , we found that recombinant Bax and Bak , proapoptotic members of the Bcl 2 family , induced mitochondrial Deltapsi loss , swelling , and cytochrome c release . ^^^ Bax induced mitochondrial changes were inhibited by recombinant Bcl xL and transgene derived Bcl 2 , antiapoptotic members of the Bcl 2 family , as well as by oligomycin , suggesting a possible regulatory effect of F0F1 ATPase on Bax induced mitochondrial changes . ^^^ Proapoptotic Bax and Bak BH 3 ( Bcl 2 homology ) peptides , but not a mutant BH 3 peptide nor a mutant Bak lacking BH 3 , induced the mitochondrial changes , indicating an essential role of the BH 3 region . ^^^ Taken together , these findings suggest that proapoptotic Bcl 2 family proteins , including Bax and Bak , induce the mitochondrial PT and cytochrome c release by interacting with the PT pores . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax protein , which acts as a death promoter message , was expressed in the ductal and acinar epithelial cells and in mononuclear infiltrating cells of SS patients compared with control individuals , while Bcl 2 , an inhibitor of apoptosis , was primarily found in the lymphocytic infiltrates . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical stains were carried out in 21 cases of Rb by use of MIB 1 , PCNA , bcl 2 , Fas , BAX , NF , SP and Tunel stains , respectively . ^^^ There are only a few positive cells by Tunel stain suggesting apoptotic cells , but diffuse positive cells by bcl 2 and Fas and completely negative by BAX gene . ^^^ From these results , it can be seen that differentiation of Rb cells may be affected by bcl 2 and Fas but not by BAX gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of EB 1089 on the expression of two oncoproteins which may regulate apoptosis , bcl 2 and bax were examined by Western analysis . ^^^ Densitometric analyses indicate that the vitamin D compounds lower the bcl 2 / bax ratio favouring increased susceptibility of MCF 7 cells to undergo apoptosis . 6 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the expression of bax protein , an apoptosis accelerator , was markedly stronger in esophagi from Zn / DFMO+ animals that showed increased apoptosis , whereas increased expression of bcl 2 , an inhibitor of apoptosis , was only seen in the highly proliferative , zinc deficient esophagus ( Zn / DFMO ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Under respiratory growth conditions , Bcl 10 ( L ) and Bcl 2 are unable to overcome yeast cell death triggered by a mutant Bax protein lacking the membrane anchor . ^^^ However , the death inhibitory proteins Bcl 10 ( L ) and Bcl 2 fail to rescue Bax delta mediated growth inhibition under conditions promoting respiration , although they bind Bax delta in the cell . ^^^ Our results open up the interesting possibility that there might exist proteins , unrelated to the Bcl 2 family , which could negate death induced by a membrane anchor free form of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NO induced cell death was enhanced by Bax expression in a caspase independent manner and prevented by the anti cell death protein Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of growth is associated with an increase in the number of cells undergoing apoptosis with concomitant elevation in the steady state levels of bax protein and drop in bcl 2 levels . ^^^ Our results suggest that the therapeutic effects of bryostatin 1 in our system do not involve alterations in levels and distribution of PKC but rather a direct upregulation of bax / bcl 2 ratios that is independent of p53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Liver biopsies from 15 early ( stages 1 and 2 ) and 14 late ( stages 3 and 4 ) cases of PBC and 15 normal cases were examined immunohistochemically for expression of p 53 , CD95 / Fas , bax , bcl 10 , bcl 2 and the proliferation marker Ki 67 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The talipexole pretreatment induced an increase in antiapoptotic Bcl 2 protein level but had no effect on levels of proapoptotic Bax , Bak , and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax ( a death promoting member of the bcl 2 gene family ) , the tumor suppressor gene product p 53 , and the ICE / ced 3 related proteases ( caspases ) have all been implicated in programmed cell death in a wide variety of cell types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A link between cell cycle and cell death : Bax and Bcl 2 modulate Cdk 2 activation during thymocyte apoptosis . ^^^ We found that Cdk 2 activation during thymocyte apoptosis can be regulated by p 53 , Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 in vivo expression in the murine thymus after apoptogenic treatments . ^^^ In order to determine if mechanisms controlling apoptosis are disturbed during neoplastic transformation in a model of murine radio induced thymic lymphomas , we have assessed the kinetics of p 53 , Bax and Bcl 2 in situ expression after induction of thymic apoptosis by irradiation or glucocorticoids at first in normal mice . ^^^ RESULTS : After hydrocortisone injection , levels of p 53 and Bax , but not Bcl 2 , expression were raised . ^^^ A whole body sublethal irradiation led to an increase of p 53 and Bcl 2 , but not Bax , expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of these cytokines was paralleled by up regulation of Fas expression and down regulation of Bcl 2 and Bcl xL but not Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results suggest that m 3 has a mutation in a specific function upstream of Bax , while ms 1 has a mutational block in the general pathway downstream of the ' Bcl 2 checkpoint ' . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : bcl 2 and bax belong to the bcl 2 related gene family , which marks a new class of genes that influence apoptosis . ^^^ AIMS : To analyse the expression of bcl 2 and bax in pancreatic cancer and correlate the results with clinical parameters . ^^^ METHODS : The levels of bcl 2 and bax mRNA expression were analysed by northern blot and the exact site of mRNA transcription was determined by in situ hybridisation . ^^^ RESULTS : Northern blot analysis indicated that , in comparison with the normal pancreas , bcl 2 mRNA was overexpressed in 30 % and bax mRNA in 61 % of the pancreatic cancer samples . ^^^ Concomitant overexpression of bcl 2 and bax was present in 26 % of the cancer samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Bax and Bcl xs , two death promoting proteins of the Bcl 2 family , were up regulated following BA treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pretreatment tumor biopsies were immunohistochemically investigated for expression of p 53 , Bcl 2 , Bax ( bcl 2 associated 10 protein ) , and Bcl 10 ( L ) ( bcl 2 related 10 protein ) . ^^^ The overall expression of p 53 , Bcl 2 , Bax , and Bcl 10 ( L ) was 52 . 6 , 57 . 9 , 100 , and 97 . 4 % respectively . ^^^ No correlations were found between the expression of Bcl 2 and Bax and the response to chemotherapy . ^^^ Additionally , a more favorable outcome was observed in tumors positive for Bcl 2 ( not significant ) , whereas no differences in survival were observed in relation to the expression of Bax or Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prolactin regulated apoptosis of Nb 2 lymphoma cells : pim 1 , bcl 2 , and bax expression . ^^^ This effect was not associated with altered expression of bcl 2 , bax , or pim 1 . ^^^ The results suggest that bcl 2 , bax , and pim 1 do not play a critical role in DEX induced apoptosis in Nb 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Bcl 2 homologous protein , Bax , promotes cell death by competing with Bcl 2 . ^^^ While Bax Bax homodimers act as apoptosis inducers , Bcl 2 Bax heterodimer formation evokes a survival signal for the cells . ^^^ Both Bcl 2 and Bax are transcriptional targets for the tumour suppressor protein , p 53 , which induces cell cycle arrest or apoptosis in response to DNA damage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of HA Bax but not Bcl 2 or Bcl XL attenuates 6 hydroxydopamine induced neuronal apoptosis . ^^^ Bax , a member of the Bcl 2 gene family , is known to promote apoptosis in many cases but to block cell death under certain conditions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The bax protein was induced and the bcl 2 protein was inhibited by KT in cells containing wild type p 53 ( Hep G 2 , COLO 205 ) but not in cells without p 53 ( Hep 3B ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alteration of bcl 2 and bax expression in embolized meningiomas . ^^^ We examined the expression of the p 53 and bcl 2 family in embolized meningiomas and investigated the interaction between them . p 53 and its downstream effector p 21 accumulated mainly in perinecrotic areas , where apoptosis was also observed . bcl 2 was often expressed in the areas distant from necrosis , whereas Bax was immunostained more intensely in the perinecrotic areas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax and p 53 gene products have been linked to programmed cell death pathways . p21WAF1 has been shown to mediate p 53 induced cell cycle arrest and to inhibit cyclin dependent kinase activity . ^^^ We found up regulation of p21WAF1 and Bax expressions , however , the expressions of p 53 and Bcl 2 genes remained unchanged in TPA treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , aggregation and toxicity were not affected by other modulators of neuronal cell death such as genetic deletion of the proapoptotic bcl 2 family member bax or addition of the protein synthesis inhibitor cycloheximide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the role of the Bcl 2 gene family has been only poorly investigated in colorectal cancer , we have examined the expression of the apoptosis blockers Bcl xL and Bcl 2 , as well as the proapoptotic factors Bax and Bak . ^^^ The Bcl 2 / Bax regulation system of cell homeostasis seems to be of lesser importance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To address the biological role of BH 3 , two Bax Bcl 2 chimeras were generated in which 13 amino acids encompassing BH 3 was swapped between anti apoptotic Bcl 2 and pro apoptotic Bax , thereby generating Bax with BH 3 of Bcl 2 ( Bax BH3Bcl2 ) , and Bcl 2 with BH 3 of Bax ( Bcl 2 BH3Bax ) . ^^^ Function and binding of the chimeras was then assessed utilizing the adenoviral Bcl 2 homologue , E1B 19K , which blocks apoptosis , and interacts with Bax , but not with Bcl 2 . ^^^ E1B 19K did not interact with Bax BH3Bcl2 but did interact with Bcl 2 BH3Bax . ^^^ Bax BH3Bcl2 retained pro apoptotic function , while Bcl 2 BH3Bax did not exhibit either pro or anti apoptotic activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Endpoints included delayed apoptosis , cloning efficiency of cells in ' healthy ' colonies and expression of the apoptosis regulatory proteins bcl 2 and BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of Bcl 2 , Bax , Bcl 10 , and Mcl 1 expression in pancreatic cancers . ^^^ We found that Bcl 2 was expressed in 23 % , Bax in 53 % , Bcl 10 in 90 % , and Mcl 1 in 90 % of the invasive ductal adenocarcinomas . ^^^ These results suggest that an imbalance between antiapoptosis proteins ( such as Bcl 2 , Bcl XL , and Mcl 1 ) and proapoptotic proteins ( such as Bax and Bcl Xs ) is involved in the distinctive biologic features of adenocarcinomas of the pancreas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Two colour cytometric analysis of permeabilized CD34+ cells stained with antibodies to Bcl 2 , Bcl 10 ( anti apoptotic ) , Bax and Bad ( pro apoptotic ) , demonstrated significantly higher ratios of pro 5 anti apoptotic proteins in early MDS ( 2 . 47 ( 1 . 19 9 . 42 ) compared to advanced disease ( 1 . 14 ( 0 . 06 3 . 32 ) , P=0 . 0001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of possible mechanisms of induction of apoptosis does not support Fas and Fas L as having a main role but points instead to Bcl 2 and Bax . ^^^ The induction of apoptosis in leukaemic T cells is characterised by the decrease of Bcl 2 and consequent predominance of Bax . ^^^ By contrast , in the normal T cells , which do not enter apoptosis , the quantitative relationship of Bcl 2 to Bax remains unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The amount of apoptosis in leg muscles was assessed by electron microscopy , by the terminal deoxynucleotidyl transferase assay and by electrophoretic detection of fragmented DNA ; the expression of Bcl 2 , Bax , Fas , ICE , p 53 and ubiquitin was examined by immunohistochemistry and Western blot . ^^^ Myofibre content of Bcl 2 decreases whereas Bax , Fas , ICE and ubiquitin modify their pattern of expression in correlation with the changes in apoptotic myonuclei . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , vitamin D compounds , although not able to induce apoptosis when used alone , potentiate apoptosis induced by 9 cis RA in HL 60 cells and differentially regulate the expression of the apoptosis related gene products bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although BAX and BCL 2 expression was similar among the three cell lines , the expression of the anti apoptotic protein , BCL 10 ( L ) , was significantly lower in S 1 cells than in S 3 and DCT cells , and this may have contributed to the heightened sensitivity of S 1 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed that the AT 2 receptor activates tyrosine phosphatase ( s ) such as mitogen activated protein ( MAP ) kinase phosphatase 1 ( MKP 1 ) and inactivates MAP kinase ( extracellular signal regulated kinase ( ERK 1 and ERK 2 ) ) , resulting in Bcl 2 dephosphorylation and up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of bcl 2 , mcl 1 / EAT , and bax were examined by Northern blot analysis and semi quantitative RT PCR method in 25 adrenal tumors , including seven adrenal pheochromocytomas ( PHE ) , seven aldosterone producing adenomas ( APA ) , four adrenal cortisol producing adenomas ( CS ) , one deoxycorticosterone producing adenoma ( DOC ) and six non hyperfunctioning adrenal cortical adenomas ( NF ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , immunoprecipitation using anti bcl 2 followed by Western blots using anti Bax showed that , unlike overexpressing cells , this bcl 2 was not complexed with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis regulatory proteins include those that block apoptosis such as Bcl 2 and Bcl 10 , whilst a related protein Bax promotes apoptosis . ^^^ Paraffin embedded samples from ten different lesions of squamous cell carcinoma ( SCC ) , Bowen ' s disease ( BD ) , keratoacanthomas ( KA ) , and nine normal adult skin samples were stained by immunohistochemistry to detect expression of Bcl 2 , Bcl 10 , Bax , Ki 67 , p21wafl , p 53 and apoptosis ( TUNEL assay ) . ^^^ These immunostaining profiles reveal that squamoproliferative lesions , including invasive transformed KCs , preferentially express Bcl 10 over Bcl 2 , in addition to upregulating their Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypothesizing that loss of basal cells in oral lichen planus is due to apoptosis , we evaluated LP specimens for apoptosis regulating proteins [ positive regulators Bcl xS , Bax , Fas / Fas ligand , p 53 , and negative regulators ( anti apoptotic ) Bcl 2 , Bcl xL and compared results with reactions in normal mucosa and chronically inflamed gingiva . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Waf 1 , Bax and Bcl 2 expression in field cancerization of the head and neck . ^^^ The aim of this study was to establish the status of p 53 gene and p 53 regulated proteins ( Bax , Bcl 2 and Waf 1 ) expression in head and neck simultaneous preneoplastic and invasive lesions from patients with chronic alcohol and tobacco exposure . ^^^ PCR SSCP analysis of p 53 gene and immunohistochemical analysis of Waf 1 , Bax and Bcl 2 proteins were performed in 30 representative microdissected samples of mucosa exposed to carcinogens and preneoplastic or invasive lesions from 8 patients . ^^^ Bax / Bcl 2 ratio was > 1 in all premalignant lesions and < 1 in tumor samples only . ^^^ A P 53 independent pathway seems to be implicated in Waf 1 , Bax and Bcl 2 expression with an inversion of the Bax / Bcl 2 ratio restricted to invasive carcinomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic induction by AN 207 , as measured by Bax and Bcl 2 protein levels , was increased in stable cells that express LH RH Rc compared with parental cells . ^^^ Clinically used LH RH antagonists partially inhibited apoptotic Bax expression and DNA fragmentation induced by AN 207 , and blocked AN 207 induced down regulation of Bcl 2 steady state protein levels . ^^^ This study demonstrates the receptor specific cytotoxic effect of 2 pyrrolinodoxorubicin conjugated to [ D Lys 6 ] LH RH , exerted through induction of apoptosis and modulation of Bax , Bcl 2 , and DNA fragmentation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bcl 10 , bax and bak in renal parenchyma , oncocytomas and renal cell carcinomas . ^^^ Expression of bcl 2 , bcl 10 , bax and bak was investigated by immunohistochemistry and Western blotting of regular and alterated renal parenchyma as well as in 57 renal cell carcinomas . ^^^ Bcl 2 , bcl 10 and in part bax were found to be overexpressed in inflammed renal parenchyma , whereas atrophic tubuli predominantly stained for bcl 2 and to a lesser degree for bcl 10 and bax . ^^^ Moderate to strong expression for bcl 2 , bcl 10 , bax and bak was found in 24 , 38 , 2 and 13 of 57 carcinomas , respectively . ^^^ Bcl 2 , bcl 10 , bax and bak expression were correlated to tumor type . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although p 53 has been shown to directly activate transcriptional bax gene and to inhibit expression of bcl 2 gene during radiation induced apoptosis , it is poorly understood how the Bcl 2 family changes in p 53 deficient cells during radiation induced apoptosis . ^^^ After 10 irradiation , expression of Bcl 2 was elevated , while Bax expression was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we evaluated levels of bcl 2 and BAX messenger RNAs ( mRNAs ) using a reverse transcriptase polymerase chain reaction ( RT PCR ) assay . ^^^ Finally , evaluation by RT PCR indicated that on postnatal Day 17 , mRNA for bcl 2 , which inhibits apoptosis , was decreased to 73 . 5 + / 11 . 4 % ( P < 0 . 001 ) of Day 5 controls ; whereas mRNA for BAX , which enhances apoptosis , was increased to 243 . 0 + / 102 . 0 % ( P < 0 . 001 ) of Day 5 values . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The induction of p 53 regulated genes including GADD 45 , p21CIP1 / WAF1 and BAX by genotoxic stress was substantially reduced in cells expressing high levels of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The p 53 gene encodes a transcription factor that contributes to several different cellular activities , including apoptosis , the cellular response to radiation , and the activation of proteins such as GADD , Bcl 2 ( represses to apoptosis ) and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The absence of modifications in Bcl 2 and Bax immunoreactivity , a lack of strong c Jun / AP 1 ( N ) immunoreactivity in vacuolated cells , and the absence of DNA breaks , as seen with the method of in situ end labeling of nuclear DNA fragmentation , all suggest that there is no involvement of the apoptotic pathway in vacuolated cells in this new neurodegenerative disorder . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study , therefore , analyzed in lesions of the oral cavity , the significance of angiogenesis in relation to apoptosis , expression of apoptosis regulatory p 53 , bax and bcl 2 proteins as well as tissue proliferation defined by cyclin D 1 expression . ^^^ Presence of a mutant p 53 , increased bcl 2 expression and altered bax expression are also involved in this complex process . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In a BCL family , BCL 2 was coexpressed in one or two nuclei in the perinuclear space of the multinuclei of MVECs , whereas MCL 1 , BCL XS / L , and BAX were all negative , indicating that the BCL 2 coding gene is expressed only in the corresponding one or two nuclei of the multinuclei . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent investigations using markers of apoptosis , ( including TUNEL , Bcl 2 and BAX ) , have established the presence of DNA damage in some neurons and in other cell types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : The purpose of this study was to determine whether there is an increase in expression of bcl 2 and related bcl 2 gene family members bcl 10 and bax in liver biopsy samples obtained from patients with either hepatitis C infection or cirrhosis . ^^^ Bcl 2 , bcl 10 , and bax , as well as other bcl 2 related proteins , function coordinately through homo and heterodimerization to regulate apoptosis . ^^^ METHODS : Sections cut from archived liver biopsy samples embedded in paraffin were probed with antibody specific for bcl 2 , bcl 10 , or bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cadmium treatment had no effects on either Bcl 2 or Bax protein expression in tumor xenografts , indicating that apoptotic pathways probably do not contribute to this anti neoplastic effect . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During this process , IL 6 downregulated expression of BCL 2 in 1A9 M cells and stimulated BCL XL expression , but had no effect on p 53 , Bax , or Bak gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased bcl 2 / bax ratio in B cell chronic lymphocytic leukemia is associated with a progressive pattern of disease . ^^^ In order to evaluate clinical implications of altered expression of bcl 2 and bax proteins in B cell chronic lymphocytic leukemia ( CLL ) we studied 27 patients with this disease . ^^^ In contrast bax expression was lower in progressive than in non progressive disease , therefore leading to a higher bcl 2 / bax ratio in patients of the former group . ^^^ If confirmed in longitudinal studies , quantitative cytofluorometric evaluation of bcl 2 and bax protein might help to identify patients with progressive disease who could possibly benefit from early therapy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cariporide moderately reduced apoptosis only in the reperfused left ventricle to values not significantly greater than those in sham operated animals , and this was associated with a significantly higher ratio of Bcl 2 to Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mechanistic studies indicated that PSP 94 enhanced the expression of proapoptotic protein Bax without affecting Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In comparison with A 1 , Bcl 2 was expressed at low levels and was up regulated by monocytes only at 21 h , while neither Bax nor Bcl xL levels were altered by monocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of cell survival during B lymphopoiesis : apoptosis and Bcl 2 / Bax content of precursor B cells in bone marrow of mice with altered expression of IL 7 and recombinase activating gene 2 . ^^^ Using immunofluorescence labeling , multiparameter flow cytometry , and a short term culture assay , we show that the apoptotic rates of precursor B cells , but not of more mature B cells , are enhanced by IL 7 gene deletion , associated with increased intracellular content of Bax and decreased Bcl 2 , while , conversely , an IL 7 transgene suppresses precursor B cell apoptosis and produces low Bax and high Bcl 2 levels . ^^^ During normal B cell development , high Bax / Bcl 2 ratios characterize cells undergoing greatest apoptotic cell death . ^^^ Pro B cells in RAG 2 / mice , all destined to abort , show elevated Bax levels and Bax / Bcl 2 ratios . ^^^ The results demonstrate that IL 7 strongly promotes in vivo cell survival and maintains antiapoptotic Bcl 2 / Bax ratios during the development of precursor B cells in mouse bone marrow . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At 10 9 M to 10 7 M , LPA and S1P significantly suppressed cellular levels of the apoptosis promoting protein Bax , without altering the levels of Bcl xL or Bcl 2 assessed by Western blots and immunoassays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression following methylazoxymethanol acetate induced apoptosis in the external granule cell layer of the developing rat cerebellum . ^^^ Since Bcl 2 protects a variety of cell types from programmed cell death , whereas Bax promotes apoptosis , the present study examines Bcl 2 and Bax proteins , and bcl 2 and bax mRNA expression in the developing cerebellum of the rat following methylazoxymethanol ( MAM ) acetate administration by using immunohistochemistry , Western blotting and Northern blotting . ^^^ Bcl 2 expression in the developing cerebellum is observed in proliferating and differentiating cells , whereas Bax expression is higher in differentiating cells than in proliferating cells during development . ^^^ Dying cells are not stained with Bcl 2 and Bax antibodies . ^^^ Furthermore , no modification in the intensity of Bcl 2 and Bax protein bands and in the intensity of Bcl 2 and bax mRNA bands on Western and Northern blots , respectively , were observed between control and treated rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no correlation between the susceptibility to gemcitabine and the endogenous expression of the B cell lymphoma 2 ( BCL 2 ) family proteins BCL 2 , BCL XL , myeloid cell leukemia 1 ( MCL 1 ) , BCL 2 associated 10 protein ( BAX ) , BCL 2 homologous antagonist / killer ( BAK ) and BCL XS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nonetheless , there were no significant alterations in levels of immunoreactive Bcl 2 , Bcl 10 ( L ) , Bax , Bad , and Bak , nor any evidence of cytochrome c release into cytosol and dissipation of delta ( psi ) m . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas Fas ligand / Fas and tumor necrosis factor ( TNF ) alpha / TNF receptor 1 levels were not altered by NOS 2 deficiency , transcript levels for p 53 were significantly lower in grafts from NOS 2 / recipients , coinciding with a significant increase in the antiapoptotic Bcl 2 / Bax balance and decrease in Bcl Xl levels . ^^^ When NOS 2 is present , p 53 might control NOS 2 mediated apoptosis by stimulating Bax and repressing Bcl 2 and Bcl Xl expression , which may activate the cell death program in the rejecting heart . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of Bcl 2 , Bcl xL / S , Bax and Bak expressions in colorectal carcinomas . ^^^ The immunohistochemical expressions of the apoptosis related proteins Bcl 2 , Bcl xL / S , Bax and Bak were investigated in tumor specimens selected from 58 consecutive patients undergoing surgery for advanced colorectal carcinoma . ^^^ In the normal colonic mucosa , Bcl 2 positive epithelial cells tended to be located at the base of the crypts , while the Bcl xL / S , Bax and Bak positive epithelial cells tended to be located at the luminal surface . ^^^ The intracellular expression patterns of Bcl 2 and Bax were diffuse cytoplasmic , whereas those of Bcl xL / S and Bak were granular cytoplasmic . ^^^ In the adenocarcinomas , the intracellular expression patterns of all antibodies were diffuse cytoplasmic , and the percentages of Bcl 2 , Bcl xL / S , Bax and Bak positive cases ( > 20 % of cancer cells labeled ) were 29 % , 43 % , 45 % and 69 % , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , p 53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines ( SKOV 3 , UL 1 , UL 3C ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fibronectin interaction with alpha4beta1 integrin prevents apoptosis in B cell chronic lymphocytic leukemia : correlation with Bcl 2 and Bax . ^^^ Quantitation of the apoptosis regulatory proteins Bcl 2 and Bax revealed that cells cultured on the H 89 fragment showed high / moderate levels of Bcl 2 ( with some interpatient variation ) and low levels of Bax resulting in an elevated Bcl 2 / Bax ratio . ^^^ These results indicate that adhesion of B CLL cells to fibronectin upregulate the Bcl 2 / Bax ratio and this may contribute to the anti apoptotic effect induced via alpha4beta1 integrin . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : BCL 2 , BCLXL , BCLXS , and BAX transcripts were amplified using reverse transcriptase polymerase chain reaction in 7 high grade gliomas ( HGGs ) , 7 ependymomas , and 6 cell lines ( 2 glioblastomas , 3 medulloblastomas , and 1 supratentorial primitive neuroectodermal tumor [ PNET ] ) . ^^^ Immunohistochemical staining for BCL 2 , BCLX , BAX , and p 53 was performed in 7 pediatric low grade gliomas ( LGGs ) and 7 pediatric HGGs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Following irradiation , Western analysis neither demonstrated any significant alteration in Bcl 2 , p29Bcl xL , p18Bcl xLdelta , or Bax ; nor induced CD 95 ( Fas receptor ) or Fas ligand expression in any cell type . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of Bcl 2 prevented cytochrome c release and reoxygenation injury without ameliorating ATP depletion or Bax translocation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The purpose of this study was to investigate apoptosis , proliferation , and the expression of apoptosis influencing proteins bcl 2 and bax and estrogen and progesterone receptors during breast carcinoma progression . ^^^ The expressions of bcl 2 , bax , and estrogen and progesterone receptors were studied immunohistochemically . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Long term lithium treatment suppresses p 53 and Bax expression but increases Bcl 2 expression . ^^^ Long term , but not acute , treatment of cultured cerebellar granule cells with LiCl induces a concentration dependent decrease in mRNA and protein levels of proapoptotic p 53 and Bax ; conversely , mRNA and protein levels of cytoprotective Bcl 2 are remarkably increased . ^^^ The ratios of Bcl 2 / Bax protein levels increase by approximately 5 fold after lithium treatment for 5 7 days . ^^^ Exposure of cerebellar granule cells to glutamate induces a rapid increase in p 53 and Bax mRNA and protein levels with no apparent effect on Bcl 2 expression . ^^^ Pretreatment with LiCl for 7 days prevents glutamate induced increase in p 53 and Bax expression and maintains Bcl 2 in an elevated state . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In search of the molecular mechanism responsible for the protective effect , we find that protection from UVA induced apoptosis is tightly correlated with NO mediated increases in Bcl 2 expression and a concomitant inhibition of UVA induced overexpression of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in glomerular disease . ^^^ Bcl 2 may account in part for the maintenance of hypercellularity in human glomerular diseases through preventing cell death and by counteracting bax which may be expressed to regulate excessive proliferation . ^^^ Thus , the balance of bcl 2 / bax expression may be critical in the course of human glomerular diseases . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of Bax and Bcl 2 expression in p 53 immunopositive breast cancers . ^^^ Bax and Bcl 2 are proteins that regulate programmed cell death and apoptosis . ^^^ In an effort to better understand the relation between p 53 and the in vivo control of the expression of Bax and Bcl 2 in adenocarcinomas of the breast , we evaluated by immunohistochemistry the expression of Bcl 2 and Bax in 149 invasive ductal carcinomas , 135 of which were chosen because of their p 53 immunopositivity . ^^^ Comparisons of the percentages of p 53 immunopositive tumor cells with the percentages of Bcl 2 and Bax positive cells ( as continuous variables ) revealed a significant inverse correlation between Bcl 2 and p 53 ( r = 0 . 41 , P < 0 . 001 ) but not between Bax and p 53 . ^^^ In the p 53 positive subset , the percentages of Bax and Bcl 2 immunopositive tumor cells were correlated positively ( r = 0 . 27 , P = 0 . 002 ) , suggesting that the expression of these genes may be co regulated to some extent in these breast cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Purified protein had an affinity for a Bax BH 3 domain peptide comparable to that for in vivo folded recombinant human Bcl 2 and suppressed caspase activation in a cell free assay for apoptosis . 1H NMR spectroscopy of rhBcl 2 , both free and complexed with the Bax BH 3 domain peptide , provided further evidence for the structural and functional integrity of the refolded protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The overexpression of Bax , a member of the Bcl 2 family , promotes cell death and the dimerization ( or oligomerization ) of Bax has been shown to be important for its function . ^^^ Furthermore , several binding assays demonstrated that Bcl XL , an anti apoptotic member of the Bcl 2 family , can bind to the oligomeric form of Bax without requiring Bax to dissociate to monomers . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All these events are prevented by p27Kip1 overexpression . p27Kip1 does not modulate Bcl 2 , Bcl 10 ( L ) , Mcl 1 and Bax protein level in leukemic cells but suppresses Mcl 1 expression decrease observed in mock transfected U 937 cells undergoing etoposide induced cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have previously shown that these cell lines express undetectable levels of the suppressor of apoptosis Bcl 2 and relatively high levels of the apoptosis inducer Bax ( Chresta et al . , 1996 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MycN overexpression and cytotoxic drugs also synergized to induce p 53 and Bax protein expression , while Bcl 2 and Bcl 10 ( L ) protein levels remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study EGF R , HER 2 / neu ( p 185 ) , p 53 , Mib 1 ( Ki 67 ) , Bax , Bcl 2 , ras expression and ploidy in borderline tumors of the ovary by assessing their frequency , and relationship to histologic type , tumor recurrence and survival . ^^^ Paraffin embedded sections were stained using monoclonal antibodies against EGF R , HER 2 / neu ( p 185 ) , p 53 , Mib 1 ( Ki 67 ) , Bax , Bcl 2 , and ras . ^^^ Expression of Bax , Bcl 2 , and ras was found in 37 % ( 20 / 54 ) , 28 % ( 15 / 54 ) and in 7 % ( 4 / 54 ) of the cases , respectively . ^^^ CONCLUSION : The data demonstrate expression of EGF R , p185 / HER 2 / neu , p 53 , Mib 1 ( Ki 67 ) , Bax , Bcl 2 , and ras in a subgroup of patients with ovarian borderline tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The relationship between cisplatin induced apoptosis and p 53 , bcl 2 and bax expression in human lung cancer cells . ^^^ OBJECTIVES : Given the roles of bcl 2 , bax and p 53 in apoptosis , we investigated the effect of their expression on the response to cisplatin in order to understand the molecular events of cisplatin resistance in lung cancers . ^^^ The endogenous levels of bcl 2 , bax and p 53 protein expression in lung cancer cells were assessed by Western blot analysis and DNA of polymerase chain reaction amplified exon 5 to 8 of p 53 gene was directly sequenced . ^^^ RESULTS : H 69 , which had bcl 2 expression , p 53 mutation and decreased expression of p 53 and bax , was relatively resistant to cisplatin and delayed and reduced apoptosis . ^^^ Although apoptosis was markedly reduced in cisplatin resistant sublines compared to their parental cells , there were no significant differences in the expression of p 53 , bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The fivefold elevation in steady state Bcl 2 concentration is not accompanied by detectable changes in the levels or cellular distributions of the related anti apoptotic regulator Bcl xL or of the proapoptotic regulators Bax and Bak and does not produce detectable effects on basal proliferation , differentiation , or death programs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We used antibodies specific for the human Bcl 2 , Mcl 1 , Bax , Bak and p 53 proteins to examine the expression of these apoptosis regulating genes in 49 archival specimens of patients with radically resected non small cell lung cancer ( NSCLC ) . ^^^ Tumour cells containing immunostaining for the antiapoptotic proteins Bcl 2 and Mcl 1 were present in 31 % and 58 % of the cases evaluated , respectively , whereas immunopositivity for the proapoptotic proteins Bax and Bak was found in 47 % and 58 % of the samples . p 53 immunopositivity was detected in 61 % of the samples . ^^^ The expression of Bcl 2 and p 53 and the expression of Mcl 1 and Bax showed a positive association ( P = 0 . 02 and P = 0 . 06 respectively ) , whereas the expression of Bax was inversely related to p 53 ( P = 0 . 008 ) . ^^^ Our results establish the frequent expression of the Bcl 2 family proteins Bcl 2 , Mcl 1 , Bax and Bak in NSCLC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 and members of the bcl 2 gene family , such as bcl 2 , bax and mcl 1 , were determined immunohistochemically with specific antibodies . ^^^ However , the extent of apoptosis did not correlate with the expression of p 53 , bcl 2 , bax and mcl 1 . ^^^ We conclude that , in the metaplasia dysplasia carcinoma sequence in the lung , the elevation of the AI % is an early event associated with cell proliferation activity , but is independent of the expression of p 53 , bcl 2 , mcl 1 and bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytofluorographic analysis and RT PCR reveal that MEC 1 and MEC 2 overexpress Bcl 2 together with Bax , express large amounts of Bcl xL and trace amounts of Bcl xS . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In MOLT 4 cells treated with Trolox after 10 irradiation , viability , DNA fragmentation , expression of p 53 , BCL 2 , BAX , active SAPK / JNK , active caspase 3 and the cleavage of PARP were measured by the trypan blue exclusion test , agarose gel electrophoresis and Western blotting . ^^^ The expression of BCL 2 and BAX , which would occur downstream from p 53 , was not changed by irradiation and Trolox treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of Bcl 2 protein family expression in undifferentiated and differentiated cells suggests that susceptibility of M 1 D cells to apoptosis may be controlled , in part , by expression of the proapoptotic alpha isoform of Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exposure of endothelial cells to hypoxia did not alter levels of proapoptotic and antiapoptotic Bcl 2 family members Bax and Bcl XL by immunoblot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cdk 2 was found to function upstream of the tumor suppressor p 53 , transactivation of the death promoter Bax , alterations of mitochondrial permeability , Bcl 2 , caspase activation , and caspase dependent proteolytic cleavage of the retinoblastoma protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
XRT was administered 24 h post infection , and Western blot analyses were conducted for p 53 , p21WAF1 / CIP1 , bax , and bcl 2 2 days after XRT . ^^^ No changes were observed for bax or bcl 2 expression with any of these treatments . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analyses of apoptosis and of the apoptosis regulatory proteins Bcl 2 , Bax , Bcl 10 , and Bad were done in 95 nontumorous and neoplastic pituitary tissues by terminal deoxynucleotide transferase mediated dUTP nick end labeling ( TUNEL ) , immunohistochemistry , and Western blotting . ^^^ The lowest levels of Bcl 2 , Bax , and Bcl 10 expression were in pituitary carcinomas as detected by immunostaining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chlorambucil resistance in B cell chronic lymphocytic leukaemia is mediated through failed Bax induction and selection of high Bcl 2 expressing subclones . ^^^ We have now monitored the in vitro viability of B CLL cells in relation to Bcl 2 and Bax expression over a 48 h time course following exposure to chlorambucil . ^^^ Taken together , these data add further weight to the suggestion that Bcl 2 / Bax ratios may be pivotal in determining the fate of B CLL cells . ^^^ Furthermore , the Bcl 2 / Bax ratios found in apoptotic B lymphocytes were remarkably similar in the treated , untreated and normal control cells , which suggests that there is a universal Bcl 2 / Bax ratio threshold for cell survival and cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , there was no difference between the two cell lines in the expression of Bcl 2 family proteins Bcl 2 , Bcl XL , Bcl XS , Bad , and Bax at the whole cell level , as analyzed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the expression of MCL 1 , an antiapoptotic member of the BCL 2 family , is downregulated during Na Sal induced cell death , whereas the expression of BCL 2 , BAX , and BCL XL is unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Spontaneous apoptosis in laryngeal squamous cell carcinoma is independent of bcl 2 and bax protein expression . ^^^ BACKGROUND : bcl 2 and bax genes are known to be involved in the control of apoptotic cell death , an important mechanism of growth regulation that influences the biologic behavior of tumors . ^^^ The aim of the current study was to investigate the relationship of bcl 2 and bax expression to the rate of spontaneous apoptosis in laryngeal carcinomas , and to assess its relations to clinicopathologic features of tumors . ^^^ METHODS : Immunohistochemical analyses for bcl 2 and bax were performed on paraffin embedded tissue sections from 134 primary laryngeal squamous cell carcinomas . ^^^ An inverse relation was found between bcl 2 and bax expression ( P < 0 . 004 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was performed to examine the expression of CD 3 , CD 20 , PCA 1 , CD 40 , CD40L , Bcl 2 , Bax , and Bcl 10 on T and B lymphocytes infiltrating labial salivary glands of SS patients . ^^^ Bcl 10 was also abundantly expressed on infiltrating mononuclear cells , but , Bax expression was relatively less than that of Bcl 2 or Bcl 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Distribution of apoptosis related proteins in the quail ovary during folliculogenesis : BCL 2 , BAX and CPP 32 . ^^^ It is suggested that follicular apoptosis is driven by the status of the BCL 2 : BAX rheostat , and that CPP 32 is a key effector of granulosa cell death . ^^^ In the present study , we have immunohistochemically localized two BCL 2 family members , BCL 2 and BAX , and one caspase , CPP 32 , in the quail ovary during folliculogenesis . ^^^ The present results support the BCL 2 : BAX rheostat hypothesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , both protective factors ( e . g . , Bcl 2 and Bcl xL ) and factors that promote hepatocyte death by apoptosis ( e . g . , Bax ) or necrosis ( e . g . , UCP 2 ) may be increased in fatty livers . ^^^ Immunohistochemistry showed striking induction of hepatocyte proteins that promote ( e . g . , Bax ) and inhibit ( e . g . , Bcl 2 and Bcl xL ) apoptosis in both groups with fatty liver . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , we examined the expression of the Bcl 10 and Bax genes , which are known to synergize and antagonize Bcl 2 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To explore the potential apoptotic nature of TGF beta 1 mediated antitumor effect against human prostate cancer cells , the expression of apoptotic proteins bcl 2 and bax was examined by Western blot analyses . ^^^ This induction of apoptosis in the LNCaP tumors with restored TGF beta 1 signaling was associated with decreased bcl 2 expression , increased bax , and caspase 1 immunoreactivty . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was found that VD 3 significantly induced the expression of Bcl 2 messenger RNA and protein in thyrocytes but had no effect on the expression of Bcl xl and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the role of such proteins in the regulation of apoptosis of eosinophils , the expression of Bcl 2 and homologues Bcl xL ( death antagonists ) , Bax , and Bcl xS ( death agonists ) were examined by immunoblot , flow cytometry , and reverse transcriptase polymerase chain reaction analysis . ^^^ Peripheral blood eosinophils were found to express constitutively Bax and Bcl 10 , but Bcl 2 was absent . ^^^ Culturing eosinophils in the presence of 100 pg / ml IL 5 for 24 h significantly reduced apoptosis ( P < 0 . 01 ) to 10 . 7 + / 2 . 6 % compared with 46 . 8 + / 7 . 4 % in the absence of IL 5 , and induced Bcl 2 mRNA and protein expression , with no detectable change in Bax , Bcl 10 , or beta actin as a control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Derangements in cell cycle control and apoptosis regulation might be responsible for the progression from metaplasia to dysplasia and adenocarcinoma We tested this hypothesis by performing cell cycle analysis , in situ detection of apoptosis , and evaluation for the immunohistochemical expression of proteins involved in proliferation ( Ki 67 ) , the control of apoptosis ( bcl 2 , bcl 10 and bax ) , and cell cycle regulation ( retinoblastoma and cyclin D 1 ) . ^^^ A statistically significant linear association was found between bcl 10 expression , bax expression , and the bcl 2 to bax expression ratio versus increasing histologic severity ( P = . 0004 , P = . 007 , and P = . 03 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twenty four formalin fixed , paraffin embedded IMTs for which clinical outcome was known were evaluated for cellularity , cytologic atypia , mitoses , ganglion like cells , inflammatory infiltrate , DNA ploidy by flow cytometric examination , and bax , bcl 2 , p 53 , and c myc expression by immunohistochemical analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Concomitant with apoptosis , elevated level of CPP 32 protease activity ( caspase 3 ) was observed , with decreases in Bcl 2 protein and increases in Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in proliferation ( Ki 67 ) , apoptosis , ER , progesterone receptor ( PgR ) , cyclin D 1 , p27kip1 , Bcl 2 and Bax expression were assessed during the 2 weeks after estrogen deprivation . ^^^ There were reductions in both Bcl 2 and Bax staining but these appeared to be greater for Bcl 2 , effectively decreasing the Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The genes encoding transforming growth factor beta receptor type 2 ( TGFbetaRII ) , insulin like growth factor 2 receptor ( IGFIIR ) , Bcl 2 associated 10 protein ( BAX ) , hMSH 3 and hMSH 6 have simple repeat sequences in their coding regions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ischemia induced increases in the cell death promoting protein , bax , may disrupt the bcl 2 / bax ratio necessary for normal neuronal functioning and thus promote transient ischemic death . ^^^ In order to locally maintain this critical bcl 2 / bax ratio and thus protect CA 1 neurons from delayed neuronal death , a herpes simplex viral ( HSV ) vector was used to selectively introduce human bcl 2 , under the control of the herpes IE 4 / 5 promoter , into the CA 1 region of the gerbil hippocampus . ^^^ Therefore , by altering the local ratio of bcl 2 / bax using the HSVbcl 2 vector one may protect CA 1 pyramidal cell from the delayed neuronal death of transient global ischemia . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of Bax , Bcl 10 , and Bcl 2 apoptotic regulatory proteins in colorectal carcinomas , and association with p 53 genotype / phenotype . ^^^ AIMS : Spontaneous apoptosis and expression of the apoptotic regulatory proteins Bax , Bcl 10 , and Bcl 2 were investigated in 50 colorectal carcinomas . ^^^ Staining intensities for Bax , Bcl 10 , and Bcl 2 were strong ; that is , equivalent to or greater than positive normal mucosal cells , in 11 of 50 , 20 of 49 , and 20 of 48 carcinomas . ^^^ Bax , Bcl 10 , and Bcl 2 protein expression were not correlated with tumour apoptosis or tumour DNA ploidy status . p 53 was expressed in 34 of 50 tumours and p 53 gene mutations were detected in 22 of 29 p 53 positive tumours analysed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry was performed on tissue sections using antibodies against bcl 2 , bcl 10 , bax , and bak . ^^^ Both bcl 2 and bax were diffusely cytosolic whereas bcl 10 and bak exhibited a distinct perinuclear distribution . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , no significant changes were detected in the expression of the mdm 2 , bcl 2 , bax , fas and fasl genes , suggesting the existence of other genes associated with apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using quantitative Western blotting we have determined the level of expression of BCL 2 , BAX , MCL 1 , and BCL 10 in lymphoblasts from 47 children with ALL ( 33 at presentation only , 4 at relapse only , and 10 at both presentation and on relapse ) . ^^^ BCL 2 , BAX , and MCL 1 were detected in all samples . ^^^ BCL 2 levels and the BCL / BAX and MCL 1 / BAX ratios were found to be significantly higher in B lineage as compared with T lineage disease ( P < . 003 , . 02 , and . 02 , respectively ) . ^^^ Kaplan Meier analysis showed a significant correlation between high BAX expression and an increased probability of relapse ( P < . 05 by the log rank test ) , suggesting that chemosensitivity in leukemic blasts may be regulated by factors that override the BCL 2 pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Methods of assaying Bcl 2 and Bax family proteins in yeast . ^^^ Certain proapoptotic members of the Bcl 2 family , Bax and Bak , have intrinsic cytotoxic activities in that they not only induce or sensitize mammalian cells to undergo apoptosis but also display a lethal phenotype when ectopically expressed in two yeast species Saccharomyces cerevisiae and Schizosaccharomyces pombe . ^^^ Furthermore , the antiapoptotic Bcl 2 and Bcl XL proteins can protect yeast against Bax mediated lethality , suggesting that the death regulatory functions of these Bcl 2 family proteins are well preserved in yeast . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 protein duels with its counteracting twin , a partner known as BAX . ^^^ When BAX is in excess , cells execute a death command ; but , when BCL 2 dominates , the program is inhibited and cells survive . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show here that transition from the pro B to pre B and then mature B cell stages of murine lymphopoiesis is accompanied by changes in proliferating cells in sensitivity to 10 irradiation induced apoptosis and that this is paralleled by variation in the ratio of anti ( Bcl 2 / Bcl chiL ) to pro ( Bax ) apoptotic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This review summarizes the recent initial studies on the in vitro channel activity of Bcl 2 , Bcl XL and Bax and offers some speculation as to the physiological role that these channels may play in the cell death pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Developmental expression patterns of Bcl 2 , Bcl 10 , Bax , and Bak in teeth . ^^^ The ontogenic profile of expression of four members of the Bcl 2 family ( Bcl 2 , Bcl 10 , Bax and Bak ) was examined in the mouse by immunohistochemistry using paraffin sections . ^^^ In general , contemporaneous co expression of the Bcl 2 and Bax proteins , and of the Bcl 10 and Bak proteins was noted in various types of cells during the developmental process , with the intensity of Bcl 2 > Bax and of Bak > Bcl 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sodium butyrate does not increase Fas receptor cell surface expression and does not modify cell levels of Bcl 2 , Bcl xL , Bcl xS and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Irradiation induces G2 / M cell cycle arrest and apoptosis in p 53 deficient lymphoblastic leukemia cells without affecting Bcl 2 and Bax expression . ^^^ Expression of Bcl 2 and Bax were not detectably altered after irradiation . ^^^ Thus , in these , and perhaps other leukemia cells , a p 53 and Bcl 2 / Bax independent mechanism is operative that efficiently senses irradiation effects and translates this signal into arrest in the G2 / M phase of the cell cycle and subsequent apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This sensitivity could be reverted to the parental phenotype by transforming the depleted cells with normal platelet mitochondria . mRNA expression of BAX , BCL 2 , MDR 1 , MRP , ERCC 1 and ERCC 2 , putatively associated with cisplatin resistance to apoptotic death was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An elevated bax / bcl 2 ratio corresponds with the onset of prostate epithelial cell apoptosis . ^^^ We studied this well characterized in vivo model of apoptosis to evaluate how the expression of two particular gene products , bcl 2 and bax , known to be important for the regulation of apoptosis were affected by castration . ^^^ Finally , SDS PAGE / Western blot analysis of bax and bcl 2 protein expression in the regressing rat prostate gland with bax and bcl 2 specific antibodies showed that the changes in the bax and bcl 2 protein levels were similar and consistent to that found for the mRNAs . ^^^ In summary , the expression of both bax and bcl 2 gene products are uniquely modulated during castration induced regression of the rat ventral prostate gland . ^^^ The changes we observed identify a transient but marked increase in the bax / bcl 2 expression ratio of the tissue that peaks on the second and third days after castration , coinciding with the peak periods of prostate cell apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this paper , we have further characterized the sensitive and resistant phenotypes and investigated whether a different expression of the apoptotic genes Fas , FasL , Bcl 2 , Bcl 10 and Bax is involved in the regulation of Dex mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we demonstrate that inhibition of the epidermal growth factor receptor tyrosine kinase activity with either an epidermal growth factor receptor antagonistic monoclonal antibody ( MoAb 425 ) or an epidermal growth factor receptor selective tyrosine kinase inhibitor ( AG 1478 ) downregulated Bcl 10 ( L ) expression in normal human keratinocytes but had no effect on expression of the pro apoptotic Bcl 2 homologs Bad , Bak , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
None of the cytokines affected expression of the proapoptotic protein Bax , suggesting that all three cytokines were specific for Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : The infection and apoptosis of CEM cells were associated with enhanced expression of Bax , Bcl 2 , Bcl 10 ( L ) and caspase 1 ( ICE ) . ^^^ The CD 8 cells of HIV infected individuals exhibited increased expression of Bcl 2 , Bcl 10 ( L ) , Bax and caspase 1 but , in contrast to the CD 4 subset , they showed elevated expression of p21CIP1 and p16INK4a compared with controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the ratio of expression of the pro apoptotic bax gene to the anti apoptotic bcl 2 gene was reduced in bcl 10 deficient cultures grown in ITS , suggesting that the interaction between these bcl 2 family members may , in part , regulate a Bcl xL independent survival pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Growth factors prevent changes in Bcl 2 and Bax expression and neuronal apoptosis induced by nitric oxide . ^^^ Incubation of neurons with SNP induced caspase 3 like activation following downregulation of Bcl 2 and upregulation of Bax protein levels in cultured neurons . ^^^ Pretreatment of cultures with either IGF 1 or bFGF prior to NO exposure inhibited caspase 3 like activation together with the changes in Bcl 2 and Bax protein levels . ^^^ These results suggest that the changes in Bcl 2 and Bax protein levels followed by caspase 3 like activation are a component in the cascade of NO induced neuronal apoptosis , and that the neuroprotective actions of IGF 1 and bFGF might be due to inhibition of the changes in the protein levels of the Bcl 2 family . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , increasing the R 1881 concentration in the presence of 2 . 0 microM 4 HPR increased apoptotic cell death . 4 HPR decreased prostate specific antigen ( PSA ) protein levels in conditioned medium and decreased PSA mRNA expression . 4 HPR also decreased the ratio of bcl 2 to bax mRNA expression in LNCaP cells by approximately 45 % , indicating that the apoptotic effects of 4 HPR may be mediated , at least in part , by alterations in the bcl 2 / bax regulated apoptotic pathway . ^^^ We concluded that ( 1 ) 4 HPR exerts growth suppressive and apoptotic effects on LNCaP cells , ( 2 ) 4 HPR can interact with androgen to suppress proliferation and induce apoptosis , ( 3 ) the apoptotic effects of 4 HPR may be mediated in part by the bcl 2 / bax pathway , and ( 4 ) a pro oxidant mechanism may contribute to the anti proliferative and apoptotic inducing effects of 4 HPR . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , addition of endostatin led to a marked reduction of the Bcl 2 and Bcl XL anti apoptotic protein , whereas Bax protein levels were unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the substantia nigra of three Parkinson ' s disease ( PD ) patients and three age matched individuals by in situ DNA end labeling ( ISEL ) and immunohistochemistry for the apoptosis regulating proteins Bcl 2 , Bax and Bcl 10 on 50 consecutive sections per patient . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 , Bcl 2 , Mcl 1 , Bax , and Bak was assessed by immunohistochemical analysis . ^^^ RESULTS : Tumor cells containing immunostaining for the antiapoptotic proteins Bcl 2 and Mcl 1 were present in 4 ( 15 % ) and 24 ( 92 % ) of the cases evaluated , respectively , whereas immunopositivity for the proapoptotic proteins Bax and Bak was found in 9 ( 35 % ) and 24 ( 92 % ) of the samples . ^^^ There was a positive correlation between the expression of Bcl 2 and Bax and between Mcl 1 and Bak . ^^^ CONCLUSIONS : Our results establish the frequent expression of the Bcl 2 family proteins Bcl 2 , Mcl 1 , Bax , and Bak in locally advanced head and neck cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the expression of the bcl 2 family oncoproteins bax , bak , bcl 2 , bcl xL , and mcl 1 in the course of differentiation of human keratinocytes cultured at low ( 0 . 15 mM ) and high ( 1 . 87 mM ) calcium concentrations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ninety archived paraffin embedded specimens from 25 patients ( two or more sequential biopsies each ) and eight control specimens were evaluated in immunohistochemically stained sections for tumor suppressor protein p 53 , p 53 binding protein mdm 2 , and apoptosis regulatory proteins Bcl 2 , Bcl 10 , Bax , and Bak . ^^^ Ten of 90 specimens were positive for Bcl 2 ( all were dysplasias or carcinomas ) , and only 2 / 90 specimens were positive for Bax . ^^^ These data show that apoptosis associated proteins are altered in variable patterns in both premalignant and malignant oral epithelial lesions . p 53 and especially Bak and Bcl 10 are expressed early ; Bax is largely absent ; and Bcl 2 and mdm 2 show sporadic expression in the development of oral premalignant and malignant disease . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunostaining with antibodies specific for p 53 , bcl 2 , bax , and MIB 1 was performed to evaluate expression of these proteins in formalin fixed , paraffin embedded specimens of 111 SCCHN patients treated with concurrent chemoradiotherapy ( carboplatin , 100 mg / m2 , four to six times every week ; total radiation therapy dose of 40 65 Gy over 4 6 . 5 weeks ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RNA expression studies , based on a multiplexed nuclease protection assay , demonstrated that cell cycle and apoptosis related genes were differentially expressed within 48 h of POH treatment ; p 21 ( Cip1 / WAF1 ) , bax , bad , and annexin 1 were induced ; cyclin E and cyclin dependent kinase 2 were repressed ; and bcl 2 and p 53 were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An increase in Bax expression and concurrent decrease in Bcl 2 expression are observed when SCC cells are exposed to C 225 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have examined , in situ , the influence of Bcl 2 over expression on the messenger RNA level of two pro apoptotic , bax and cpp 32 , and one anti apoptotic , bcl xl , regulators of neuronal death . ^^^ Thus , fatal axotomy induces cell death genes bax and cpp 32 messenger RNAs neosynthesis which is not prevented by athanatal Bcl 2 over expression . ^^^ This suggests that the protective effect of Bcl 2 results from interactions with Bax and CPP 32 at the post translation level without repercussion at the messenger RNA level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Evaluation of these cultures showed that E 1 ( ) E 4 ( + ) Ad vectors provide an `` antiapoptotic ' ' signal that , in association with an increase in the ratio of Bcl 2 to Bax levels , induces the endothelial cells to enter a state of `` suspended animation , ' ' remaining viable for at least 30 days , even in the absence of serum and growth factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , bcl 2 and their homologues regulate a distal step in an evolutionary very well conserved pathway of apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The extent of apoptosis was defined by morphological criteria and the TUNEL ( terminal deoxy nucleotidyl transferase mediated dUTP biotin nick end labelling ) assay . p 53 , bax , bcl 2 and cyclin D 1 expression was evaluated by immunocytochemistry . ^^^ There was also correlation between TUNEL reactivity and immunoreactivity of apoptosis regulatory proteins . p 53 ( r = 0 . 641 , P = 0 . 00023 ) , bcl 2 ( r = 0 . 642 , P = 0 . 00014 ) and bax ( r = 0 . 651 , P = 0 . 00002 ) . ^^^ Significant correlation was evident between the bax / bcl 2 ratio and TUNEL ( r = 0 . 652 , P = 0 . 00001 ) as well as between cyclin D 1 and TUNEL reactivity ( r = 0 . 577 , P = 0 . 00001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This process is , in part , regulated by the ratio of PCD inducers ( Bax ) or inhibitors ( Bcl 2 ) . ^^^ An abnormally high ratio of Bcl 2 to Bax prevents PCD , thus contributing to resistance to chemotherapeutic agents , many of which are capable of inducing PCD . ^^^ First , we determined whether bcl 2 and bax mRNA were expressed in three morphologically distinct NSCLC cell lines : NCI H 226 ( squamous ) , NCI H 358 ( adenocarcinoma ) , and NCI H 596 ( adenosquamous ) . ^^^ All three NSCLC cell lines expressed both bcl 2 and bax mRNA and had functional PCD pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytoprotective effects of hematopoietic growth factors on primary human acute myeloid leukemias are not mediated through changes in BCL 2 , bax , or p21WAF1 / CIP1 . ^^^ Although HGF can modulate the expression of apoptosis regulatory genes , including bcl 2 , bax , and p21WAF1 / CIP1 in cell lines , few data address whether HGF regulate the expression of these proteins in primary acute myeloid leukemia ( AML ) . ^^^ MATERIALS AND METHODS : We evaluated the expression of bcl 2 , bax , and p21WAF1 / CIP1 in primary samples from patients with AML in the presence and absence of HGF . ^^^ RESULTS : While a combination of steel factor ( SF ) and PIXY 321 inhibited etoposide induced apoptosis in 8 / 11 primary AML samples studied , Bcl 2 and bax protein levels were unaffected by exposure to HGF and / or etoposide . ^^^ CONCLUSIONS : These data indicate that HGF mediated inhibition of chemotherapy induced apoptosis in primary AML samples is not mediated through changes in Bcl 2 , bax , and p21WAF1 / CIP1 protein levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similar to self limited tumour growth , proliferation of atherosclerotic lesions in their cellular phase are controlled by p 53 , P 21 and the gene products of MDM 2 , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Susceptibility of differentiated thyrocytes in primary culture to undergo apoptosis after exposure to hydrogen peroxide : relation with the level of expression of apoptosis regulatory proteins , Bcl 2 and Bax . ^^^ We have studied 1 ) the kinetics and the amplitude of the apoptotic response to H2O2 and 2 ) the relationship between the extent of the apoptosis inducing effect of H2O2 , the H2O2 degradation activity , and the level of expression of apoptosis regulatory proteins , Bcl 2 and Bax , in pig thyrocytes in primary culture . ^^^ During the same period of culture , the Bcl 2 cell content progressively decreased , whereas that of Bax concomitantly increased ; thus , the Bcl 2 / Bax ratio varied from about 6 on day 1 to 0 . 5 on day 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : The prognostic significance of spontaneous levels of apoptosis and Bcl 2 , Bax , and Bcl 10 protein expression in follicular center lymphoma ( FCL ) is unknown . ^^^ The objectives of this retrospective study were ( 1 ) to investigate the relationship between pretreatment apoptosis levels and long term treatment outcome in patients with Stage 1 and 2 FCL ; ( 2 ) to define the incidence and patterns of Bax and Bcl 10 protein expression in human FC ; and ( 3 ) to determine the relationship of Bcl 2 , Bax , and Bcl 10 expression with spontaneous apoptosis levels and clinical outcome in localized FCL . ^^^ Expression of Bcl 2 , Bax , and Bcl 10 proteins was assessed using immunohistochemistry . ^^^ Expression of Bcl 2 , Bax , or Bcl 10 did not correlate with AI or clinical outcome . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting analysis showed that sequential treatment ( MALD / TPA ) increased Bcl 2 oncoprotein expression , whereas Bcl XL and Bax proteins were not changed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis of bcl 2 gene family member ( bax , bcl 2 , bclX s / l ) expression showed no correlation with HGF treatment , suggesting that HGF mediated apoptosis is bax independent . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and the expression of Bax and Bcl 2 in squamous cell carcinoma and adenocarcinoma of the uterine cervix . ^^^ The expression of Bcl 2 and Bax proteins was examined by immunohistochemical staining with appropriate antibodies . ^^^ CONCLUSIONS : Apoptosis appears to occur in the cancerous cells of invasive adenocarcinoma of the uterine cervix in association with a high level of expression of Bax but not of bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 homology 3 ( BH 3 ) domain is crucial for the death inducing and dimerization properties of pro apoptotic members of the Bcl 2 protein family , including Bak , Bax , and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family members Bcl 2 and Bcl xL suppress programmed cell death , whereas Bax promotes programmed cell death . ^^^ An increase in Bcl 2 but not Bcl xL or Bax , cleavage of caspase 1 , up regulation and cleavage of caspase 3 , and evidence for DNA fragmentation with both apoptotic and necrotic morphologies were found in tissue from traumatic brain injury patients compared with controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a pro apoptotic member of the Bcl 2 family , translocates from the cytosol to the mitochondria during programmed cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ability of ceramide to activate the apoptotic protease caspase 3 in HL 60 cells but not in B CLL cells , as well as the lack of correlation of ceramide mediated killing of different B CLL isolates with expression of the apoptosis regulating proteins bcl 2 and bax reinforce the conclusion that ceramide killing of B CLL cells is by a non apoptotic mechanism . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mediated by GAPDH binding , DES increases mitochondrial BCL 2 and BCL xL levels and decreases BAX levels thereby preventing the permeability transition pore ( PTP ) form opening and preventing apoptotic degradation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mutation of threonine 169 did not affect the binding of Bax to Bax , Bcl 2 , or Bcl 10 ( L ) . ^^^ This is the first report of the functional significance of missense mutations in BAX , or any other proapoptotic member of the Bcl 2 family , in primary human tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunostainings for the Bcl 2 family revealed no expression of Bax , Bad or Bak protein in any components , whereas Bcl 2 protein was detected in the periphery of basaloid cell layer , but not in the transitional or shadow cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the pro apoptotic proteins Bax , Bak and Bad , as well as the death suppressors Bcl 10 , Bcl 2 and Bcl w , are synthesised in mouse mammary gland , and dynamic changes in the expression profiles of these proteins occurs during development . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Reciprocal changes in the expression of Bcl 2 and Bax in hypoglossal nucleus after axotomy in adult rats : possible involvement in the induction of neuronal cell death . ^^^ Bcl 2 and Bax have been identified as inhibitor and promoter proteins , respectively , of apoptosis . ^^^ We analyzed the expression of Bcl 2 and Bax immunohistochemically in the hypoglossal nuclei of the adult rats following axotomy . ^^^ Quantitative analysis of the signal intensity for Bcl 2 and Bax in individual neurons showed that Bax immunostaining significantly increased 7 days after axotomy , while the intensity of Bcl 2 immunostaining decreased in most of Bcl 2 positive neurons . ^^^ Our results confirmed the occurrence of motoneuron cell death in adult rats after axotomy , and that a close temporal relationship exists between the reciprocal changes in Bcl 2 / Bax expression and the loss of motoneurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of oncoprotein Bcl 2 and bax protein in the infiltration zone and in the parenchyma of primary glia derived supratentorial neoplasms . ^^^ According to the recent studies the proper functional relation of bcl 2 and bax proteins may play a major role in one of the possible pathways leading to cell survival or to cell death . ^^^ We found that in the infiltration zone neurocytes and reactive glial cells had a strong expression of bcl 2 and weak expression of bax protein . ^^^ Thus , we have concluded that the use of bcl 2 and bax can be a useful tool in the evaluation of survival activity of non neoplastic cells in the infiltration zone and of the neoplastic cells within the tumour core . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using RT PCR it was shown that mRNA for several modulators of apoptosis ( Fas , Bcl 2 , Bcl xl , Bax , and ICE ) are expressed by both cell lines and ex vivo tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human keratinocyte terminal differentiation was accompanied by time related changes in the expression of cellular proteins involved in the control pathways of apoptosis , including downregulation of Bcl 2 and p 53 and upregulation of Bax , which coincided with the appearance of morphological signs of apoptosis . ^^^ E 6 expression led , however , to a significant reduction in cell stratification and cell death by apoptosis , which correlated with prolonged expression of Bcl 2 and reduced elevation of Bax levels that occurred concomitant with a complete loss of p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Like Bcl 2 , the biochemical mechanism of E1B 19K function includes binding to and antagonization of cellular proapoptotic proteins such as Bax , Bak , and Nbk / Bik . ^^^ A novel protein , Btf ( Bcl 2 associated transcription factor ) , that interacts with E1B 19K as well as with the antiapoptotic family members Bcl 2 and Bcl xL but not with the proapoptotic protein Bax was identified . btf is a widely expressed gene that encodes a protein with homology to the basic zipper ( bZip ) and Myb DNA binding domains . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Coimmunoprecipitation experiments showed that Bax Bax interactions are greater in the mitochondrial enriched membrane compartment of ALS motor cortex compared with controls , whereas Bax Bcl 2 interactions are lower in the membrane compartment of ALS motor cortex compared with controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : 10 irradiated Molt 4 cells and cell extract ( CE ) were used to analyse : ( 1 ) induced apoptosis ( Giemsa stain ) , ( 2 ) p 53 , Bcl 2 and Bax expressions ( immunoblot ) , ( 3 ) mitochondrial potential deltapsi ( m ) and ( 4 ) [ Ca2+ ] 1 ( flow cytometry ) , ( 5 ) caspase 3 activity , and ( 6 ) roles of [ Ca2+ ] and caspase 3 mediated pathways by inhibiting either or both pathways for induced apoptosis . ^^^ After 5Gy , p 53 was accumulated that upregulated Bax but which repressed Bcl 2 with time , resulting in a 7 fold increase in Bax / Bxl 2 at 6 h . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this lesion we identified apoptosis of vascular smooth muscle cells ( VSMCs ) by in situ DNA labelling and electron microscopy in the neointima on the 14th day after injury . mRNA expression levels of bcl 2 , bax , bcl 10 , p 53 and caspase 1 were determined by the reverse transcriptase polymerase chain reaction method both in injured and uninjured carotid arteries . ^^^ Neither bcl 2 nor bcl xl mRNA expression was detected in either injured or uninjured arteries , whereas bax and p 53 mRNA expression was identified and their mRNA levels were not altered after balloon injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recently , the proto oncogenes bcl 2 and bax have emerged as important regulators of the apoptotic form of cell death . ^^^ We examined UV irradiation elicited apoptosis and regulation of bcl 2 and bax expression both in vivo in human skin and in vitro in HeLa cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using in situ hybridization , we have studied the kinetics of expression of cpp 32 , bax and bcl xl mRNAs after a fatal lesion of the facial nerve in wild type and Bcl 2 transgenic mice , where cell death is known to be prevented . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The beta 2 adrenoceptor agonist clenbuterol modulates Bcl 2 , Bcl xl and Bax protein expression following transient forebrain ischemia . ^^^ Bcl 2 , Bax and Bcl xl proteins were detectable in the non ischemic hippocampus and the striatum . ^^^ Bcl 2 was up regulated in both detected regions at 24 h after ischemia , while the increase in Bax and Bcl xl protein expression had appeared already at 6 h and also 24 h after ischemia . ^^^ Clenbuterol not only increased Bcl 2 expression in the non ischemic hippocampus and striatum , but also up regulated Bcl 2 and down regulated Bax expression in the ischemic hippocampus and striatum . ^^^ The increase in the ratio of Bcl 2 and Bax may contribute to the anti apoptotic effect of clenbuterol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found up regulation of Bax and p21WAF1 expressions and down regulation of Bcl 2 and p 53 expression in genistein treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that auristatin PE up regulated the expression of wt p 53 , p21WAF1 and Bax , and down regulated Bcl 2 and cyclin B in HPAC cells , while only up regulation of p21WAF1 and Bax was observed in PANC 1 cells . ^^^ These results suggest that auristatin PE may induce apoptosis and p21WAF1 expression through p 53 dependent or independent pathways , and that up regulation of p21WAF1 and Bax and down regulation of Bcl 2 may be the molecular mechanism through which auristatin PE inhibits cell growth and induces apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 family proteins ( Bcl 2 , Bcl 10 , Bcl XL , Bcl Xs , BAX , BAD , MCL 1 ) and of Interleukin 1 converting enzyme ( ICE ) related proteins ( ICE , CPP 32 , ICH 1 ) was analyzed in acute leukemia cells by flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Collectively , these data indicate that the gonadotropin mediated inhibition of apoptosis in rabbit luteal cells involves enhanced expression of the oxidative stress response gene , manganese containing SOD , whose protein product may then function to protect luteal cells directly from the damaging effect of reactive oxygen species and / or indirectly by acutely down regulating expression of Bax , a prooxidant member of the Bcl 2 protein family . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no evidence showing that changes in the expressions of Bcl 2 , Bcl xL , Bak , and Bax lead directly to IFN alpha mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Diverse stimuli did not alter beta actin ( internal control ) expression , but increased calpain expression , and the upregulated bax ( pro apoptotic ) / bcl 2 ( anti apoptotic ) ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A complex mechanism of programmed cell death has been postulated , involving such factors as Fas , Bax and Bcl 2 ; tumour necrosis may also play a role . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis for Bcl 2 family of proteins showed that Bax was expressed at a 2 . 2 times lower level in 11ad cells than in KHM 11 cells while there was no difference in expression of Bcl 2 , Bcl Xs nor Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of other BCL 2 family members including BAX , BCL 2 , BCL 10 or MCL 1 were not consistently modulated by mutant or wild type p53val135 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Compared with ATP depleted controls , prior heat stress inhibited the pro apoptotic changes in the ratio of Bcl 2 to BAX , proteins known to regulate the apoptotic set point in renal cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Primary bovine glomerular endothelial cells were stimulated with TNF alpha or LPS , and apoptotic cell death was investigated by DNA fragmentation analysis , morphological studies , measurement of cytochrome c efflux and mitochondrial permeability transition , Bak , Bad , Bax , Bcl 2 , Bcl xL protein expression , and caspase 3 like protease activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both H2O2 and O 2 induced apoptosis of cardiomyocytes were associated with an increase in p 53 protein content , whereas protein levels of Bax and Bcl 2 were unaltered . ^^^ Furthermore , H2O2 elicited translocation of Bax and Bad from cytosol to mitochondria , where these factors formed heterodimers with Bcl 2 , which was followed by the release of cytochrome c , activation of CPP 32 , and cleavage of poly ( ADP ribose ) polymerase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of BAX and BCL 2 proteins remain unaltered during boswellic acid induced apoptosis . p 21 expression is induced by boswellic acids via a p 53 independent pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent results from our laboratory have indicated that ischaemia induced acute renal failure is associated with up regulation of two anti apoptotic Bcl 2 proteins ( Bcl 2 and Bcl XL ) in the damaged distal tubule and occasional up regulation of Bax in the proximal tubule . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the induction of apoptosis by phloretin , the expression of Bax protein in B 16 cells increased and the levels of p 53 , Bcl 2 , and Bcl XL proteins did not change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Gain of Bcl 2 is more potent than bax loss in regulating mammary epithelial cell survival in vivo . ^^^ The impact of gain of Bcl 2 function on mammary epithelial cell survival was compared with loss of Bax function during the two stages of mammary gland involution . ^^^ Partial loss of Bax was ineffective but increased cell survival 2 . 4 fold when combined with Bcl 2 gain . ^^^ Gain of Bcl 2 function is more potent than loss of Bax function in regulating mammary epithelial cell survival in vivo . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , M 21 melanoma cell interactions with denatured collagen , a known ligand for alpha ( 5 ) beta 3 , caused a 5 fold increase in the relative Bcl 2 : Bax ratio , an event thought to promote cell survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , and p 53 proteins in carcinogenesis of squamous cell lung cancer . ^^^ The expression of Bcl 2 , p 53 , and Bax in very early lung cancers or precancer lesions is not been fully understood . ^^^ RESULTS : The expression of the Bcl 2 and p 53 protein increased along with the advance of the morphological atypia in bronchial epithelial cells , whereas no difference in the Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Graves Basedow disease goiter : a model of Bax Bcl 2 regulated apoptosis . ^^^ This study demonstrates the involvement of a Bax Bcl 2 dependent apoptotic process in Graves Basedow thyroid disease , a pathological condition known for its spontaneously oscillating evolution . ^^^ Moreover , the thyroid follicle by accumulating apoptotic cells and bodies , allowed a tentative chronological ordering of apoptosis steps in correlation with Bax Bcl 2 tissue distribution and cellular pattern . ^^^ Our observations suggest that the initiation of apoptosis corresponds to a loss of cellular cohesion , a drop in Bcl 2 expression , and a delocalization of Bax from a putative Golgi storage location to a mitochondrial distribution . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some members of this family , such as Bcl 2 , inhibit apoptosis , whereas others , such as Bax , induce it . ^^^ The present study was performed to determine the apoptosis rate and mRNA and protein expression for Bax and Bcl 2 in the gastric mucosa of duodenal ulcer ( DU ) patients with H . pylori infection before and after H . pylori eradication . ^^^ The apoptosis was analyzed by means of terminal deoxyribonucleotide transferase mediated digoxigenin 11 deoxyuridine triphosphate biotin nick end labeling ( TUNEL ) staining , and the expression of mRNA for Bax and Bcl 2 by reverse transcription polymerase chain reaction ( RT PCR ) and Southern blot . ^^^ The infection was associated with significantly upregulated expression of mRNA and protein for Bax and suppressed mRNA and protein expression for Bcl 2 , as determined using RT PCR and Western blot analysis . ^^^ After eradication the Bax mRNA expression was still at an increased level , whereas the Bcl 2 mRNA expression remained suppressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Following etoposide exposure , cells were examined for cell cycle arrest , formation of hyperdiploid cells , apoptotic DNA degradation , loss of plasma membrane integrity , levels of expression of members of the Bcl 2 protein family , caspase activation , degradation of poly ( ADP ribose ) polymerase and movement of Bax from cytosol to cellular membrane fractions . ^^^ Surprisingly , Bcl 2 also blocked Bax membrane insertion . ^^^ In addition , Bcl 2 also prevented the increase in cellular levels of Bak , Bax and Bcl xL , along with degradation of actin and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased cardiomyocyte apoptosis and changes in proapoptotic and antiapoptotic genes bax and bcl 2 during left ventricular adaptations to chronic pressure overload in the rat . ^^^ Expression of the proapoptotic gene bax increased , whereas that of antiapoptotic gene bcl 2 decreased in LVH and LVD compared with SH . ^^^ LVH and LVD were accompanied by a dramatic cardiomyocyte upregulation of the proapoptotic gene bax and reduced bcl 2 / bax ratio , predisposing cardiomyocytes to apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical and molecular analysis of bax , bcl 2 and p 53 genes in laryngeal squamous cell carcinomas . ^^^ BACKGROUND : We investigated the role of apoptosis and its potential alterations in laryngeal squamous cell carcinomas ( LSCCs ) by evaluating bax , bcl 2 and p 53 protein expression in 50 cases and by characterising the molecular status of the bax and p 53 genes . ^^^ MATERIAL AND METHODS : p 53 and bax gene mutations were investigated by means of PCR / SSCP and direct DNA sequencing , and bax , bcl 2 and p 53 protein expression by means of immunohistochemistry . ^^^ RESULTS : We identified p 53 gene mutations in 17 / 50 cases ( 34 % ) ; p 53 expression in 26 of the 50 cases ( 52 % ) ; bcl 2 expression in 5 / 50 cases ( 10 % ) ; bax expression in 32 / 47 cases ( 68 % ) . 18 / 33 cases with a wild type p 53 gene overexpressed p 53 protein : 12 cases ( approximately 66 % ) were bax+ / bcl 2 . ^^^ CONCLUSIONS : Our observations suggest that the overexpression of p 53 may contribute to the repression of bcl 2 and the induction of bax expression in LSCCs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in Bcl 2 and Bax expression in rat tongue during 4 nitroquinoline 1 oxide induced carcinogenesis . ^^^ Bax forms homodimers and also heterodimers with Bcl 2 . ^^^ The function of the Bax Bax dimer in active cell death is antagonized by Bax Bcl 2 heterodimers . ^^^ Thus , the ratio of Bcl 2 and Bax should control the susceptibility of cells to those stimuli that induce apoptotic cell death . ^^^ In the present study , the changes in Bcl 2 and Bax expression in the tissue during carcinogenic transformation were examined immunohistochemically by means of the 4 nitroquinoline 1 oxide ( 4NQO ) induced carcinoma model . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify the relationship between the cell cycle and apoptosis , we investigated expression of p 27 , cyclin D 1 and apoptosis related proteins ( p 53 , Bax , Bcl 2 and c Myc ) in 60 cases of oral and oropharyngeal squamous cell carcinoma ( SCC ) using an immuno histochemical approach , and evaluated spontaneous apoptosis in vivo . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of Bcl 2 , Bax , and Bak expression in thyroid glands from patients with subacute thyroiditis . ^^^ To clarify a role of apoptosis and the expression of Bcl 2 family proteins in the pathogenesis of subacute thyroiditis ( SAT ) , we evaluated the expression of Bcl 2 , Bax , and Bak by immunohistochemistry and apoptosis by in situ end labeling of fragmented DNA in thyroid tissues from 11 patients with SAT . ^^^ Bcl 2 , Bak , and Bax were strongly expressed in the granulomas and regenerating thyroid follicular cells from patients with SAT . ^^^ Bcl 2 and Bak , but not Bax , were expressed in follicular cells from normal controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined Bcl 2 , Bax and p 53 expression in transitional epithelium , benign lesions derived from transitional epithelium ( Brunn ' s nests and inverted papillomas ) and transitional cell cancer ( TCC ) of the upper urinary tract and urinary bladder using immunostaining of cryostat sections . ^^^ Immunohistochemical staining showed that Bcl 2 was expressed only on basal layer cells , whereas Bax expression was restricted to superficial layers in normal transitional epithelium . ^^^ Taken together , 16 ( 17 % ) TCCs were positive for Bcl 2 , 62 ( 64 . 6 % ) TCCs were positive for Bax and 28 ( 29 % ) TCCs were positive for p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Co treatment did not significantly alter Bcl 2 , Bcl xL , Bax or Fas receptor ( FasR ) , but modestly increased Fas ligand ( FasL ) protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of constitutively activated Stat 3 correlates with altered Bcl 2 / Bax expression and induction of apoptosis in mycosis fungoides tumor cells . ^^^ The decreased ability of Stat 3 to bind DNA precedes dynamic alterations in the expression of anti apoptotic Bcl 2 and pro apoptotic Bax proteins ( decreased Bcl 2 expression and increased Bax expression ) and induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of Bcl 2 , Bax and Survivin genes in uterine cancer . ^^^ To investigate the role of the apoptosis related genes , Bcl 2 , Bax and Survivin genes were analyzed . ^^^ In 4 cervical and 2 endometrial cancer cell lines , the ratio of Bcl 2 to Bax expression was higher than the control ratio using Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The alteration of volume of lesioned cortical region , regional cerebral blood flow ( CBF ) , bcl 2 and bax expression at penumbra area were studied . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Histological measures include terminal vellus and anagen other ratios , hair shaft diameter , and morphologic features ( routine and immunohistochemical detection of bcl 2 , bax , p 53 , Ki 67 , cyclin D 1 , and hsp 70 ) before and after treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When BCL 2 and BAX protein levels were assessed using immunofluorescence , both proteins were detected in zygotes and in blastocysts . ^^^ This result supports a model in which the ratio of BCL 2 to BAX is altered in apoptotic embryos . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Downregulation of Bcl 2 , but not of Bax or Bcl 10 , is associated with T lymphocyte apoptosis in HIV infection and restored by antiretroviral therapy or by interleukin 2 . ^^^ The role of Bcl 2 , Bax , and Bcl 10 in the apoptosis of T lymphocytes in HIV infected individuals was investigated . ^^^ In contrast to Bcl 2 , no correlation is detectable between Bax or Bcl 10 expression and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exposure of undifferentiated U 937 cells to 50 microM etoposide for 6 h , that triggers apoptosis in 80 % cells , activates procaspase 2L , 3 and 8 , induces the mitochondrial release of cytochrome c and decreases Mcl 1 expression without modifying Bcl 2 , Bcl xL and Bax protein levels . ^^^ Exposure of TPA differentiated U 937 cells to 0 . 8 microg / ml cycloheximide for 24 h , that triggers apoptosis in 50 % cells , activates procaspase 2L , 3 and 8 , induces the mitochondrial release of cytochrome c and decreases Bcl xL expression without modifying Bcl 2 , Mcl 1 and Bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Colocalization of BAX and BCL 2 in small intestine and kidney biopsies with different degrees of DNA fragmentation . ^^^ Here , this `` apoptotic gradient ' ' is used to demonstrate the presence of BAX and BCL 2 proteins in the cytoplasm of cells at the onset of apoptosis . ^^^ In semithin serial sections of the small intestine , BAX , BCL 2 and DNA fragmentation were demonstrated . ^^^ BAX and BCL 2 are always colocalized and only in cells with fragmented DNA . ^^^ The gradient of BAX or BCL 2 staining is similar to the gradient of DNA fragmentation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , p21 / waf , bcl 2 , bax , Rb and Ki 67 proteins in colorectal adenocarcinomas . ^^^ This study investigated the combined immunoexpression of p 53 , p 21 , bcl 2 , bax , Rb and Ki 67 proteins in colorectal adenocarcinomas and correlated expression patterns with tumour stage and grade . ^^^ Paraffin sections from 98 cases of colorectal adenocarcinomas were stained by immunohistochemistry for p 53 , p 21 , bcl 2 , bax , Rb and MIB 1 ( Ki 67 ) proteins . ^^^ P 53 , p 21 , bcl 2 , bax , Rb and Ki 67 proteins were detected in at least 5 % of tumour cells in 63 / 98 , 72 / 98 , 52 / 98 , 96 / 98 and 98 / 98 adenocarcinomas , respectively . ^^^ Comparative study of the normal adenoma carcinoma tissues revealed abrogation of the normal immunotopography in adenomas and adenocarcinomas , and considerable modifications , increase or reduction , of the expression of p 53 , p 21 , bcl 2 , bax , Rb and Ki 67 proteins in adenocarcinomas when compared with normal mucosa and adenomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax , a stimulator of apoptosis , and Bcl 2 , an inhibitor of apoptosis , were quantitated by western blotting . ^^^ Accordingly , Bax expression was 2 . 7 fold higher in control rats , whereas Bcl 2 expression was 3 . 1 fold higher in PhIP treated rats , both changes being statistically different ( Student ' s t test , P < 0 . 05 ) . ^^^ Immunohistochemistry further confirmed a lower expression of Bax and higher expression of Bcl 2 in secretory alveolar epithelial cells of the PhIP treated mammary gland . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression in human myopathies . ^^^ Expression of the apoptosis related proteins Bcl 2 and Bax was analysed by means of immunohistochemistry in muscle biopsies from 13 patients suffering from different muscular diseases ( inclusion body myositis n=4 , polymyositis n=3 , Becker muscular dystrophy n=4 , necrotizing myopathy n=2 , and controls n=4 ) , in an attempt to learn about the role of these proteins in human muscle diseases associated with muscle fiber necrosis and regeneration . ^^^ Increased Bcl 2 and Bax immunoreactivity was observed as fine granular precipitates in the cytoplasm or as subsarcolemmal aggregates in pathological cases . ^^^ Increased Bcl 2 and Bax immunoreactivity was detected in some necrotic fibers , regenerating fibers , ring fibers and some apparently normal muscle fibers . ^^^ In addition , increased Bcl 2 and Bax immunoreactivity was observed in the periphery of rimmed vacuoles and in muscle fibers with abnormal mitochondria in patients suffering from inclusion body myositis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax and Bcl 10 expression following kainic acid administration at convulsant doses in the rat . ^^^ To assess the involvement of members of the Bcl 2 family in cell death or survival , immunohistochemistry , western and northern blotting to Bcl 2 , Bcl 10 and Bax , and in situ hybridization to Bax were examined at different time points after kainic acid treatment . ^^^ Dying neurons in the pyramidal cell layer of CA 1 and CA 3 areas , entorhinal and piriform cortices , and amygdala also expressed Bcl 2 , Bax and Bcl 10 following excitotoxicity , although many dying cells did not . ^^^ Western blots disclosed no modifications in the intensity of the bands corresponding to Bcl 2 , Bcl 10 and Bax , between control and kainic acid treated rats . ^^^ The present results suggest that cell death or survival does not correlate with modifications of Bcl 2 , Bax and Bcl 10 protein , and messenger RNA expression , but rather that kainic acid excitotoxicity is associated with Bax translocation to the nucleus in a subset of dying cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax and Bcl 2 proteins was investigated by immunohistochemical staining . ^^^ CONCLUSIONS : The current investigation indicates that low doses of RT result in apoptotic cell death in ISCC in association with the increased expression of Bax but not with increased Bcl 2 expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , bcl 2 , bax , cyclin D 1 , Ki 67 and E 6 protein was evaluated by immunocytochemistry . ^^^ Expression of bax and the bax / bcl 2 ratio showed an inverse correlation to histological grade ( p=0 . 0003 and 0 . 0001 , respectively ) . ^^^ Lowest levels of bax / bcl 2 ratio was also associated with HPV 16 and 18 infection ( p=0 . 0001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a death promoting member of the Bcl 2 family of proteins , triggered cell death when expressed in plants from a tobacco mosaic virus vector . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax protein is widely known as a pro apoptotic Bcl 2 family member that when overexpressed can trigger apoptosis in multiple cell types and is important for the developmental cell death of neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resistance of Crohn ' s disease T cells to multiple apoptotic signals is associated with a Bcl 2 / Bax mucosal imbalance . ^^^ Hence , the Bcl 2 / Bax ratio was significantly higher in CD and lower in UC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the mitochondrial factors Bcl 2 , Bcl 10 , and Bax was determined because each has been implicated in the regulation or release of cytochrome c at the level of the mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the pro apoptotic proteins ( Bax , Bak ) and anti apoptotic proteins ( Bcl 2 , Bcl 10 , Mcl 1 ) was studied by immunohistochemistry in 110 invasive ductal breast carcinomas . ^^^ Overall , Bcl 2 , Bcl 10 , Mcl 1 , Bax , Bak , ER , and p 53 were detected in 62 , 75 , 68 , 75 , 60 , 68 and 26 per cent of the cases respectively , but at different levels in each case . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of the anti apoptotic bcl xL transcript and the pro apoptotic bax mRNA was also detected but no alterations in bcl 2 or bag 1 mRNA levels were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate signal transduction mechanisms for the effect of integrins on cell survival in hepatic stellate cells , the expression of p 53 , Bcl 2 , and Bax was analyzed . ^^^ Incubation with soluble GRGDS peptide resulted in increased expression of p 53 and decreased the Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated the expression of apoptosis related factors , p 53 , Bax , Bcl 2 , and spontaneous apoptosis in 57 cases of oral and oropharyngeal squamous cell carcinoma ( SCC ) by immunochemical staining and ApopTag kit . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Genotoxic chemicals not only damage cellular DNA , but may also induce cell apoptosis if they are lethal to the cell . p 53 , Bcl 2 and Bax play important roles in the regulation of genotoxic chemical induced cell apoptosis . ^^^ Since the mechanisms by which cellular DNA damaged by different DNA damaging chemicals may not be the same , we studied the involvement of p 53 , Bcl 2 and Bax in apoptosis induced by methyl methanesulfonate ( MMS ) and hydrogen peroxide ( H2O2 ) . ^^^ Lethal MMS treatment also increased Bax , but not Bcl 2 expression , whereas in H2O2 induced apoptosis , the level of both Bcl 2 and Bax declined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have investigated the association of bax with microsomal , nuclear and mitochondrial membranes using a cell free system and found , contrary to bcl 2 , that bax binds poorly to these organelles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of the present study is to clarify ( 1 ) whether cardiac apoptosis occurs during the transition from compensated hypertrophy to decompensated heart failure , and ( 2 ) whether expression of the genes encoding Bax ( an apoptosis inducer ) and Bcl xL and Bcl 2 ( apoptosis inhibitors ) is altered during this transition . ^^^ The expression of bcl 2 , bcl xL and bax was analysed by Northern blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic extracts from 293 cells cleave Bcl 2 but not Bax , even though Bax is cleaved to an 18 kDa fragment in SK NSH cells treated with ionizing radiation . ^^^ In contrast to Bcl 2 , cleavage of Bax was only partially blocked by caspase inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The incidence of apoptotic nuclei ( < 1 % ) as evidenced by the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) method , and the histological distribution of immunoreactive Bcl 2 , Bax , and Bcl 10 proteins , were similar in placentae collected after delivery and before the onset of labor and in placental explants maintained overnight at 4 degrees C in a minimal salt Hepes medium . ^^^ This marked increase was associated with a decrease in the intensity of the Bcl 2 immunostaining and an increase in the intensity of Bax and Bcl 10 immunostaining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No significant changes in Bcl 2 , BclX , and Bax protein expression level were observed in the transfected clones as compared with the control H 460 cells whereas a 2 fold increase in Bak protein levels was noticed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression in naevi and melanomas and their relation to ploidy status and proliferation . ^^^ Twenty five naevi , 53 primary melanomas , 36 melanoma metastases were stained immunohistochemically for the presence of Bcl 2 , Bax and Ki 67 antigen in order to define the relation between apoptosis regulators and proliferative activity . ^^^ Bax demonstrated a tendency to increase and Bcl 2 was decreasing along with melanoma progression . ^^^ In the group of euploid cases Bcl 2 showed a strong significant correlation with Bax expression ( p = 0 . 0001 ) and both Bcl 2 and Bax correlated with Ki 67 expression and S phase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect was specific to bcl 2 , as expression of other members of the bcl 2 family ( bax , bcl 10 ( L ) , bcl 10 ( S ) , and bad ) was unaffected by estradiol treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas hyperthermia exerted no effect on the expression of Bcl 2 and Bax , heat induced a > 30 fold increase of tumor necrosis factor ( TNF ) alpha mRNA expression and a significant increase in TNF alpha protein secretion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis revealed that the transfected cell line showed overexpression of both bcl 2 and bax , and that PDT caused selective destruction of bcl 2 , leaving bax unaffected . ^^^ The greater apoptotic response by the transfected line is , therefore , attributed to the higher bax : bcl 2 ratio after photodamage . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We tested an unselected panel of 11 melanoma cell lines for sensitivity to CD 95 and the corresponding expression of CD 95 , CD95L , bcl 2 , bcl 10 , bcl xS , bax and FLIP proteins . ^^^ Apoptosis related proteins CD95L , bcl 2 , bcl 10 , bcl xS and bax were found to be heterogenously expressed in different melanoma cell lines tested . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Histopathological analysis of apoptosis , and expression of p 53 , bcl 2 , bax , and Ki 67 in laryngeal squamous cell carcinomas and dysplasia . ^^^ The purpose of this study is to determine the frequency of apoptosis in laryngeal carcinomas and to examine its relationship to the pathological parameters , including ki 67 expression , and to expression of p 53 , bcl 2 , and bax protein . ^^^ Expressions of p 53 , bcl 2 , and bax , i . e . apoptosis related genes , and ki 67 , a proliferation marker , were immunohistochemically examined . ^^^ No relationship was found between apoptotic index , ki 67 index , and expression of p 53 , bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to define the roles of p 53 in acquisition of drug resistance in ATL cell lines , we prepared several ATL cell lines which differed in sensitivity to adriamycin ( ADM ) , and examined their functional p 53 statuses , expressions of p 53 , p 21 , Bcl 2 , Bax , and cell cycles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In situ localization of p 53 , bcl 2 and bax mRNAs in rat ocular tissue . ^^^ We mapped p 53 , bcl 2 and bax mRNAs , which are known to have correlation with apoptotic cell death , in the rat ocular tissue . p 53 mRNA signals were present in several layers of retina , optic nerve , corneal epithelium , ciliary process and posterior surface of the iris . ^^^ While almost the same pattern as p 53 was recognized in the case of bcl 2 mRNA , bax signals were not found in ocular tissue except for ganglion cell layer ( GCL ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family of proteins plays a central role in the regulation of apoptotic cell death by suppressing the apoptosis while some others such as Bax proteins promote this process . ^^^ In the present study , the gastric epithelial apoptosis was determined by means of expression of Bax and Bcl 2 mRNA in the gastric mucosa following acute stress . ^^^ The apoptosis rate in the gastric mucosa was determined by the terminal deoxynucleotidyl transferase ( TDT ) mediated 2 deoxyuridine 5 triphosphate ( dUTP ) biotin nick end labeling ( TUNEL ) staining method and the expression of Bax and Bcl 2 mRNA was analyzed by RT PCR and southern blot hybridization . ^^^ The enhancement in gastric epithelial apoptosis accompanies the mucosal damage induced by stress and this appears to be triggered by the shift from the cell death effector Bax to the cell death repressor Bcl 2 protein . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mammary gland tissue , similar to many other tissues , expresses a number of different Bcl 2 relatives including bcl 10 , bax , bak , bad , bcl w , bfl 1 , bcl 2 as well as the bcl 2 binding protein Bag 1 . ^^^ Bcl 2 expression in human breast cancers has been associated with a good prognosis , while decreased Bax expression has been linked to poor clinical outcome . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Acute treatment of B6C3F1 mice with phenobarbital resulted in increased levels of bcl 2 and decreased levels of bax protein , while acute treatment with WY 14 , 643 resulted in increased bcl 2 and BAG 1 protein in the liver . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also found an up regulation of Bax and p21WAF1 expression and down regulation of Bcl 2 and c erbB 2 in genistein treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It is generally accepted that Bcl 2 exerts its antiapoptotic effects mainly by dimerizing with proapoptotic members of the Bcl 2 family such as Bax and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of this study was to investigate the presence of bcl 2 , Bax and oestrogen receptor proteins in secretory endometrium collected from ten patients with normal ovulatory cycles 4 or 6 days after the LH surge , and on the same days in a subsequent cycle in which the formation of secretory changes was inhibited by the administration of the antiprogestin mifepristone ( RU 486 ) 2 days after the onset of the LH surge . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neuron loss is not associated with increased vulnerability to nuclear DNA fragmentation , and nor is it accompanied by modifications in the expression of the proteins Bcl 2 and Bax , and transcription factors c Fos and c Jun , thus suggesting that these proteins are probably not involved in cell death in these disorders . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , cells expressing Tr PKCepsilon were resistant to apoptosis , which was associated with higher Bcl 2 levels , a marked impairment in p 53 stabilization , and dampened expression of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of expression levels of Bcl 2 protoncogene family members ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and Bax ) showed a downregulation of Bcl 10 ( L ) and Mcl 1 after CD 22 ligation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptotic proteins after depsipeptide exposure ( 0 . 015 micromol / L ) included no change in bcl 2 , elevation of bax , and decreased expression of p 27 . ^^^ These data demonstrate that depsipeptide has significant selective in vitro activity against human CLL cells concurrent with favorable alterations of the bcl 2 : bax protein ratio and decrease in p 27 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , the expression of four apoptosis related proteins ( bcl 2 , bcl 10 , bax and p 53 ) in 53 cases of HD was examined and the data were correlated with the genotype , the EBV status and the phenotype ( B , T or null ) of the neoplastic cells . ^^^ The H RS cells were bcl 2 positive in 19 / 53 ( 36 % ) , bcl 10 positive in 17 / 53 ( 32 % ) , bax positive in 1 / 53 , and p 53 positive in 41 / 53 ( 77 % ) cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , we found that this effect is mediated in the absence of changes in expression of Bcl 2 , Bcl Xl , and the pro apoptotic protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , an apoptosis promoting member of the bcl 2 family , may be a key factor influencing the chemosensitivity of tumor cells , however , its involvement in cellular sensitivity to anti cancer drugs remains uncertain in squamous cell carcinoma ( SCC ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased apoptosis in c myc hepatocytes was accompanied by increased p 53 , bax , and bak and decreased bcl 2 protein levels . ^^^ Phenobarbital , a potent liver tumor promoter , inhibited apoptosis in c myc hepatocytes but not in wild type hepatocytes , decreased p 53 and bax , and increased bcl 2 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Endogenous Apaf 1 also failed to coimmunoprecipitate with endogenous Bcl 2 or Bcl 10 ( L ) , or with two proapoptotic relatives ( Bax and Bim ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , the expression of bcl 2 family members ( bcl 2 , bax , bad , and bcl 10 ( s / l ) ) and caspases 1 , 2 , 3 , 4 , and 6 was investigated through a range of stages of chick lens development using immunocytochemistry , Western blotting , and affinity labelling for caspases using biotinylated caspase inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In VSMC transfected with antisense Rb ODN , bax , a promoter of apoptosis , was significantly increased in VSMC transfected with antisense Rb ODN ( P < 0 . 01 ) , whereas bcl 2 and Fas did not play a pivotal role in the induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To address a potential role for p 53 , Bcl 2 associated protein 10 ( Bax ) , and apoptosis in the processes associated with cell turnover during cystic medial degeneration ( CMD ) of the aorta . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Calcium ionophore , ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl 2 and Bax expression levels . ^^^ Bcl 2 and Bax expression levels in HT 1376 after ionomycin treatment were examined by Northern and Western blot analyses . ^^^ Ionomycin treatment caused a marked decrease in the ratios of Bcl 2 to Bax mRNA and protein in HT 1376 cells . ^^^ CONCLUSIONS : These findings suggest that ionomycin based therapy could be used as a novel therapeutic strategy for advanced bladder cancer through the effective induction of apoptosis by decreasing the ratio of Bcl 2 to Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX translocation is a critical event in neuronal apoptosis : regulation by neuroprotectants , BCL 2 , and caspases . ^^^ Overexpression of Bcl 2 neither altered the normal subcellular localization of BAX nor prevented its redistribution with deprivation but did inhibit the subsequent release of cytochrome c , caspase activation , and cell death . ^^^ BCL 2 acts primarily , if not exclusively , at the level of mitochondria to prevent BAX mediated cytochrome c release , whereas NGF , KCl , or cAMP may abort the apoptotic program at multiple checkpoints . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax was more highly expressed in cells cultured at high density , and on collagen vs . matrigel , whereas changes of Bcl 2 and Fas expression observed in culture appeared unrelated to the rate of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In using immunocytochemistry to seek putative death factors , we observed that squamous epithelial cells of the tongue were negative for Bax , a death factor in the Bcl 2 family of survival / death factors , and were also negative for p 53 , a tumor suppressor protein linked to apoptosis and Bax transcription . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similar changes in the expression of apoptosis effector genes , p 53 , and p 53 responsive genes p21Cip1 / WAF 1 / Sid1 ( p 21 ) , gadd 45 , bcl 2 and bax were observed in normal and XPD LCLs after treatment with doxorubicin , indicating that delayed apoptosis was not a consequence of defective transcription of these genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical staining with MIB 1 and for Bcl 2 and Bax enabled possible regulatory pathways to be investigated . ^^^ Bcl 2 expression was weak or absent in basal cells , and Bax was localized to upper prickle cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Drug induced apoptosis and p 53 , BCL 2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro . ^^^ Surgical specimens were examined for the frequency of apoptotic cells [ apoptotic index ( AI ) ] by a terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling method and for the expression of p 53 , BCL 2 and BAX by immunohistochemical staining . ( 2 ) Normal human diploid fetal lung fibroblast , IMR 90 and SV 40 transformed IMR 90 were exposed to 5 FU . ^^^ Apoptotic cells were detected by flow cytometry and BCL 2 and BAX mRNAs by real time quantitative RT PCR analysis . ^^^ RESULTS : ( 1 ) No significant difference in the AIs or in BCL 2 and BAX scores was observed between the 5 ' DFUR treated and control groups . ^^^ However , in the p 53 negative subgroup ( n = 36 ) , AI and BAX scores were higher and BCL 2 scores lower in the 5 ' DFUR group than in the control group ( P = 0 . 006 , 0 . 008 and 0 . 050 , respectively ) . ( 2 ) The sensitivity of IMR 90 was significantly decreased by SV 40 transformation and the 5 FU induced cytotoxicity was mainly due to induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the mutant c Jun clones exhibit increased apoptosis and elevated bax : bcl 2 ratios on expression of wild type p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential effects of chemotherapeutic agents on the Bcl 2 / Bax apoptosis pathway in human breast cancer cell line MCF 7 . ^^^ The present study explored the effects of three commonly used chemotherapeutic agents on the Bcl 2 / Bax apoptosis pathway and the interaction of these chemotherapeutic drugs with the estradiol mediated regulation of this pathway . ^^^ Our results showed that : ( 1 ) Treatment of MCF 7 cells with Adriamycin resulted in time and concentration dependent decreases in Bcl 2 and increases in Bax mRNA and protein levels . ( 2 ) Camptothecin elicited similar trends on Bcl 2 and Bax as Adriamycin , while etoposide , at 50 100 fold ( 1 5 microM ) the effective concentration of Adriamycin and camptothecin , only resulted in an increase in Bax mRNA levels . ( 3 ) Adriamycin and camptothecin , but not etoposide , were effective in suppressing estradiol stimulated increases in Bcl 2 mRNA levels . ^^^ Our study provides evidence that the Bcl 2 / Bax apoptosis pathway may be differentially regulated by chemotherapeutic agents . ^^^ In addition , interaction between these agents and estradiol on the Bcl 2 / Bax apoptosis pathway may also exist . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
E2F 1 overexpression was associated with a marked decrease in MDM 2 levels and no evidence of increased Bax levels , whereas p 53 and Bcl 2 levels remained undetectable . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , in 46 gliomas ( including astrocytomas , oligodendrogliomas , oligo astrocytomas , and glioblastomas ) , the relationship of apoptosis and necrosis and the expression of apoptosis promoting ( p 53 , bax , Fas , Fas L ) and inhibiting ( bcl 2 ) factors as well as of different isoforms of NOS ( NOSb , NOSe , NOSi ) and manganese superoxide dismutase ( MnSOD ) were studied . ^^^ Glial tumour cells displayed upregulation of bax , Fas , Fas L , p 53 , and bcl 2 but with no significant correlation with malignancy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PROBLEM : To determine the role of apoptosis regulating genes bax and bcl 2 in reproduction . ^^^ RESULTS : The bcl 2 family of apoptotic regulatory gene products interact and form dimers of anti and pro apoptotic proteins ( e . g . , bcl 2 and bax respectively ) , the ratio of which determines cell death or survival . ^^^ Menses is associated with increased apoptosis in the glands , a change in bcl 2 : bax ratio and increased levels of the pro apoptotic cytokine TNFalpha . ^^^ An increased bcl 2 : bax ratio and apoptosis in the syncytiotrophoblast characterizes failing first trimester pregnancies . ^^^ CONCLUSION : The ratio of bcl 2 : bax is crucial in the regulation of apoptosis , particularly in the human placenta . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study the possible roles of bcl 2 family proteins in oncogenesis and cytodifferentiation of odontogenic epithelium , the expression of bcl 2 , bcl 10 and bax proteins was examined immunohistochemically in tooth germs and various types of ameloblastoma . ^^^ In ameloblastomas , bcl 2 and bcl 10 proteins were expressed intensely in peripheral columnar cells and weakly in central polyhedral cells , while bax protein reactivity was low . ^^^ Basal cell ameloblastomas showed intense reactivity for bcl 2 and bcl 10 proteins , but slight or no reactivity for bax protein . ^^^ Malignant ameloblastomas showed decreased reactivity for bcl 2 protein and increased levels of bax protein as compared with benign ones . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax heterodimerizes with Bcl 2 , an inhibitor of apoptosis , and the ratio of Bax to Bcl 2 determines cellular apoptosis or survival . ^^^ Thus , we investigated apoptosis and the expressions of Bax , bax mRNA , and Bcl 2 in human arteriovenous ( AV ) fistulas used for hemodialysis , a representative of arteriosclerosis of the aforementioned type . ^^^ Bax and Bcl 2 were detected by immunohistochemistry and Western blot analysis . ^^^ Western blot analysis of Bax or Bcl 2 and in situ hybridization of bax mRNA confirmed the immunohistochemical data . ^^^ The apoptosis is probably induced by an increase in the Bax to Bcl 2 ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Susceptibility to apoptosis measured by MYC , BCL 2 , and BAX expression in arterioles and capillaries of adult spontaneously hypertensive rats . ^^^ We analyzed the expression of c myc , bcl 2 , and bax and in situ end labeling DNA fragmentation in vascular smooth muscle cells of arterioles and endothelial cells of arterioles and capillaries . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of BAX and BCL 2 expression in breast cancer cells by chemotherapy . ^^^ In this study we investigated regulation of BAX and BCL 2 expression by VP 16 and cyclophosphamide as a potential mechanism for the induction of breast cancer cell death induced by this regimen . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
With the combination , the expression of Bax and Bcl xS , the apoptosis enhancing proteins , was more up regulated and that of Bcl 2 and Bcl xL , the apoptosis suppressing proteins , was more down regulated compared to the use of EPA or TNP 470 alone , suggesting that their synergistic effect was due to an acceleration of apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the expression of the autosomal apoptosis regulator genes , BAX , bcl 2 , p 53 , and cyclic AMP responsive element modulator ( CREM ) in testis biopsies from infertile men demonstrated that BAX , bcl 2 , and p 53 immunoreactivity was absent irrespective of seminiferous tubule histology . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we demonstrate that constitutive expression of bcl xl but not bcl 2 , bcl xs , bak , bad , or bax was associated with apoptosis resistance after IL 2 deprivation in CTLL 2 cells that expressed Tax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , VK 2 treatment induced down regulation of BCL 2 and up regulation of BAX protein expression with concomitant activation of caspase 3 ( CPP 32 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl xL and Bcl 2 were protective without any change in the level of endogenous Bcl xL or Bax and inhibited hepatic caspase 3 like activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic protein bcl 2 was apparently up regulated in A549 / UCN cells , however , bcl xL , another antiapoptotic protein , was down regulated , without changes in bak and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immunoreactivities of Bcl 2 , Bax , and p 53 were assessed immunohistochemically in the kidneys . ^^^ The increased Bax , the increased p 53 , and the decreased Bcl 2 immunoreactivities were detected in H or O treated , but not in M treated , rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further characterize MPP ( + ) induced cell death and to explore the role of Bcl 2 related proteins in this death paradigm , we utilized a mesencephalon derived dopaminergic neuronal cell line ( MN9D ) stably transfected with human bcl 2 ( MN9D / Bcl 2 ) , its C terminal deletion mutant ( MN9D / Bcl 2Delta22 ) , murine bax ( MN9D / Bax ) , or a control vector ( MN9D / Neo ) . ^^^ In this death paradigm , overexpression of Bcl 2 but not its C terminal deletion mutant attenuated MPP ( + ) induced cell death whereas overexpression of Bax had no effect . ^^^ Taken together , these data indicate that ( 1 ) MPP ( + ) induces a distinct form of cell death which resembles both apoptosis and necrosis ; and ( 2 ) full length Bcl 2 counters MPP ( + ) induced morphological changes and cell death via a mechanism that is dependent on de novo protein synthesis but independent of cytosolic calcium changes , Bax expression , and / or activation of caspase ( s ) in MN9D cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Developing mice hearts on days 11 to 16 of gestation were studied using in situ end labeling of degraded DNA ( TUNEL ) , immunocytochemistry of regulatory genes Bcl 2 and Bax , and light and electron microscopy . ^^^ Immunocytochemical reaction for the apoptosis checkpoint proteins in the ventricular wall showed clearly more Bcl 2 positive cells than Bax positive cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify this mechanism , bcl 2 and bax expression were evaluated in relation to mutations of p 53 . ^^^ Immunohistochemistry was performed to evaluate bcl 2 and bax expression , and polymerase chain reaction single strand conformation polymorphism following direct sequencing was performed to investigate p 53 status . ^^^ A total of 79 carcinomas were bcl 2 positive , 146 carcinomas were bax positive , and 72 carcinomas had missense mutations of p 53 . ^^^ The frequency of a bcl 2 / bax ratio of > / =1 was significantly lower in tumors with mutations of the loop sheet helix motif than that in tumors with wild type p 53 ( P = 0 . 0240 ) . ^^^ The bcl 2 / bax ratio status was a significant factor for a prognosis in patients with NSCLC ( P = 0 . 0083 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expressions of Bcl 2 , Bax and Bak proteins in liver tissues of hepatitis B patients and their significance ] . ^^^ OBJECTIVE : To investigate the expressions of Bcl 2 , Bax and Bak proteins in liver tissues of hepatitis B patients and their relations to apoptosis and necrosis of hepatocytes . ^^^ METHODS : TUNEL assay and immunohistochemistry were used to detect hepatic apoptosis , Bcl 2 , Bax and Bak proteins expressions on hepatocytes respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic cell death , bax and bcl 2 expression during sheep mammary gland involution . ^^^ Involution in ovine mammary tissue was studied by light and electron microscopy , and bax and bcl 2 protein distribution was examined by immunohistochemistry from the last day of lactation until the 8th day of drying off . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Unlike other proapoptotic Bcl 2 family members , such as Bax and Bak , Bcl 10 ( S ) does not seem to induce cell death in the absence of an additional death signal . ^^^ Deletion mutants of Bax and Bcl 2 , which lacked BH 1 and BH 2 domains but contained a BH 3 domain , were able to antagonize the survival effect conferred by Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to test the hypothesis that increased apoptotic activity is connected with neuroendocrine differentiation and low differentiation degree in large cell carcinoma ( LCLC ) and is regulated by bcl 2 family proteins , we analysed the extent of apoptosis and tumor necrosis and their relation to the expression of bcl 2 , bax , bak and mcl 1 in 35 LCLCs , of which 20 were classified as large cell neuroendocrine lung carcinomas ( LCNEC ) and 15 as large cell non neuroendocrine lung carcinomas ( LCNNEC ) . ^^^ The extent and intensity of expression of the bcl 2 , bax , bak and mcl 1 proteins were studied by immunohistochemistry . ^^^ Immunohistochemically detected bax , bcl 2 and bak expression was independent of apoptotic index in both tumor types , while there was a statistically significant positive association between mcl 1 expression and apoptotic index in LCNNEC but not in LCNEC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The prognostic value of spontaneous apoptosis , Bax , Bcl 2 , and p 53 in oral squamous cell carcinoma of the tongue . ^^^ BACKGROUND : Bax , Bcl 2 , and p 53 proteins are involved in the regulation of apoptosis and have been reported to correlate with prognosis in several tumor types . ^^^ METHODS : Bax , Bcl 2 , p 53 , and the level of spontaneous apoptosis were evaluated in formalin fixed , paraffin embedded pretreatment specimens from 85 T 1 4 squamous cell carcinomas ( SCCs ) of the tongue by immunohistochemical methods . ^^^ For Bax and Bcl 2 evaluation , the fraction of tumor cells stained and the staining intensities were given scores that were added together , resulting in a final score . p 53 immunostaining was expressed as a percentage of positive cells . ^^^ Whereas low AI scores and low Bax expression correlated significantly with poor prognosis ( P = 0 . 0053 and P = 0 . 0012 , respectively ) , a low Bcl 2 expression was associated with a favorable clinical outcome ( P = 0 . 0262 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Oral squamous cell carcinoma is associated with decreased bcl 2 / bax expression ratio and increased apoptosis . ^^^ Expression of bcl 2 and bax and apoptosis were studied in fresh frozen samples of normal oral epithelium ( OE , n = 7 ) and oral squamous cell carcinomas ( OSCC , n = 16 ) by immunohistochemistry and the TUNEL method . ^^^ We conclude that , in OSCC , compared with OE , there is a decreased bcl 2 expression , a lowered bcl 2 / bax ratio and increased apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunocytochemistry and Western blotting we determined the expression of P glycoprotein ( Pgp ) , multidrug resistance associated protein ( MRP ) , glutathione S transferase pi ( GST pi ) , Bcl 2 , Bax , topoisomerase ( Topo ) 1 , 2 alpha and 2 beta in the human prostate cancer cell lines PC 3 , TSU Pr 1 , DU 145 and LNCaP derivatives LNCaP R , LNCaP LNO and LNCaP FGC . ^^^ The expression of Bcl 2 was restricted to TSU Pr 1 , whereas Bax was found in all cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results indicate that the activation of bcl 2 family genes such as bcl Xs and bax plays a crucial role in modulating apoptotic cell death in the sensitivity to anticancer drugs , and suggest that these proapoptotic genes might interact in the up regulation for activating downstream signals leading to apoptosis in gastric cancer cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 protein expression decreased , while bax protein expression increased from day 9 after transplantation . ^^^ Moreover , altered expression of bcl 2 and bax was accompanied by the increased proteolytic cleavage of poly ( ADP ribose ) polymerase ( PARP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of antiapoptotic bcl 2 and proapoptotic bax genes was reduced in K 562 VP16 cell line , while the content of bcl 2 mRNA was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No difference in the treatment induced modulation of proteins involved in cell cycle progression ( cyclin A , cdk 2 , pRB , E2F 1 ) and apoptosis ( bcl 2 , bax ) was observed in the three clones . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Comparison of BCL 2 and BAX protein expression with in vitro sensitivity to ARA C and 6TG in AML . ^^^ Activity of BCL 2 protein may be antagonised by BAX protein expression , thereby affecting cellular sensitivity to chemotherapeutic drugs . ^^^ The ratio of BCL 2 to BAX may be more relevant clinically , therefore cells from a further 9 patients were analysed for both proteins . ^^^ Whilst there was no overall relationship between BCL 2 / BAX ratios and sensitivity to ara C and 6TG , individual patients could be identified whose blast cells were resistant to ara C and had high BCL 2 / BAX ratios . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The decision between survival or death in response to an apoptotic stimulus is determined and regulated in part by oncoproteins which include proteins of the Bcl 2 family ( bcl 2 , bax , bcl xL ) and bcr abl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SMN was strongly expressed cytoplasmically in chronic peripheral neuropathies , in about 80 % of chronically denervated , very atrophic muscle fibers containing clumps of TUNEL positive pyknotic nuclei : about 60 % of those fibers also had cytoplasmic Bcl 2 and Bax immunoreactivity . ^^^ In regenerating muscle fibers of various myopathies SMN co localized with desmin , Bcl 2 and Bax ; it was also present at the postsynaptic domain of normal human neuromuscular junctions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of epidermal growth factor receptor ( EGF R ) and Bcl 2 was used as an indicator of stromal cell proliferation / survival , whereas Bax and the occurrence of apoptosis provided an index of cell death . ^^^ On the other hand , DB regression ( Days 14 21 ) was marked by an elevation in endogenous PKC activity and Bax expression ; EGF R and Bcl 2 were suppressed . ^^^ Additionally , antiprogestin ( RU 486 ) given on Day 9 induced PKC activity and Bax expression within 6 h and suppressed Bcl 2 and EGF R . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , but not Bax , correlates with estrogen receptor status and tumor proliferation in invasive breast carcinoma . ^^^ Bcl 2 and Bax have been demonstrated to be associated with apoptosis in breast carcinoma , and the ratio between Bax and Bcl 2 seems to be an important determinant of cellular sensitivity to induction of apoptosis . ^^^ However , little information is available on the relationship between Bcl 2 , Bax and the proliferative activity of breast carcinoma . ^^^ The purpose of this study was to investigate the significance of apoptosis related genes bcl 2 and Bax and their correlation with expression of p 53 , tumor proliferation defined by MIB 1 expression and estrogen receptor status . ^^^ Immunohistochemistry was performed to determine Bcl 2 , Bax , p 53 , estrogen receptor ( ER ) and MIB 1 expression in paraffin embedded tissues of 177 invasive breast cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of members of the Bcl 2 family of proteins , such as Bcl 2 , Bcl xL , Bax , and Bad , was unchanged by the overexpression of PHGPx in cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of the anti apoptotic protein Bcl 2 in MCF 7 cells inhibited SST induced apoptosis upstream of acidification by inhibiting p 53 dependent induction of Bax as well as by raising the resting pH ( 1 ) and attenuating SST induced decrease in pH ( 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the p 53 , Bax , and bcl 2 proteins and proliferating cell nuclear antigen ( PCNA ) were determined by immunohistochemical staining . ^^^ The expression of Bax protein was significantly greater in tumors with the wild type p 53 gene after chemotherapy . bcl 2 protein expression did not relate to p 53 gene status before or after chemotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intact plasmid DNAs were recovered by PCR amplification from the slices with bcl 2 or bcl 10 cDNAs but not from slices with empty vector or bax cDNA that promotes cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proteins of the Bcl 2 family that are known to inhibit apoptosis in mammalian cells overcome Bax induced growth arrest in yeast . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , changes in the expression of bcl 2 and bax were examined by western blot analysis . ^^^ In retinal cells going to apoptosis regardless of the stimulus , bcl 2 expression was decreased and bax expression was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax regulation of apoptosis in germ cells during prenatal oogenesis in the mouse embryo . ^^^ In this study we examined by immunohistochemistry and immunoblotting the expression of the anti and pro apoptotic proteins Bcl 2 and Bax in primordial germ cells and fetal oocytes during pre natal oogenesis in the mouse embryo . ^^^ While Bcl 2 and Bax were not detectable in primordial germ cells in vivo , both proteins were upregulated when they undergo apoptosis in culture . ^^^ These findings indicate that whereas a balance between Bcl 2 and Bax might regulate apoptosis of proliferating primordial germ cells under a partial control by SCF , Bax mediated apoptosis in meiotic oocytes may be due to intrinsic meiotic checkpoints which act to monitor aberrant DNA recombination rather than to a growth factor dependent process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The GnRH Ag induced decrease in the 18 kDa PBR protein also correlated with the reduction in the Bcl 10 ( L ) , but not Bcl 2 ( cell survival ) , gene product levels and the increase in the Bax ( cell death ) gene product expression in the luteal mitochondrial preparations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Release of mitochondrial cytochrome c into the cytosol was detectable immediately and accumulated over 2 hours after treatment while cytosolic levels of the proapoptotic Bcl 2 family member , Bax , decreased reciprocally over the same time period . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and its homologues , Bcl xL , Bcl xS , Bax , Bad , Bak and Bag 1 , was detected in all NHL cases , with wide variations between histological subtypes and within each subtype . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have found that , in addition to Bcl 2 and Bax , the expression levels of apoptosis inducers ( Bad , Bak ) and inhibitors ( Bcl xL , Mcl 1 ) were highly variable in blasts from 78 children with newly diagnosed acute lymphoblastic leukemia ( ALL ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 and bcl xL was higher and the expression of bax was lower in lower density cultures compared to higher density cultures at basal condition . ^^^ After incubation with stressors , bcl 2 and bcl xL expressions decreased and bax expression increased in both lower and higher density cultures . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The continuous high expression of Bcl 2 and the high ratio of Bcl 2 to Bax in the eyes of EMIU may possibly contribute to prevention of ocular tissue damage , and of inflammatory cells from undergoing apoptosis , thus resulting in chronic recurrent inflammation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Roles of p 53 , c Myc , Bcl 2 , Bax and caspases in glutamate induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor . ^^^ By using flow cytometric analysis , we examined the involvement of p 53 , c Myc , Bcl 2 and Bax in the glutamate induced cell death in cultured cortical neurons . ^^^ The temporal profile of p 53 , c Myc , Bcl 2 , Bax expression and caspases activation after glutamate treatment suggest that Bcl 2 , c Myc and caspase 3 play important roles in the excitotoxic neuronal cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 , bax , bcl 2 , and caspase 3 , which play critical roles in apoptotic signaling , was examined by Western blot analysis . ^^^ Expression of bax and active forms of caspase 3 was increased in the Ang II+PD 123319 group , whereas the expression of p 53 and bcl 2 was not significantly different in all groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Also , bcl 2 protein expression was downregulated in much greater extent than that of bax showing that depression of bcl 2 might be an important step during the EPA induced apoptosis in HL 60 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As Bcl 2 related proteins are involved in apoptotic process , we also evaluated their role by examining the expression of Bcl 2 , Bcl 10 ( L ) , and Bax on SEB FK 506 treated murine splenic T cells . ^^^ Although SEB injection slightly increased the expressions of Bcl 2 and Bax on V138+ T cells , FK 506 did not modulate Bcl 2 or Bax expression in these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Morphine treated Jurkat cells also showed a decreased expression of bcl 2 and an enhanced expression of bax . ^^^ The change in ratio of bax and bcl 2 seems to tilt the balance toward apoptosis , leading to the activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate if degeneration of motor neurons in amyotrophic lateral sclerosis ( ALS ) is related to altered levels of the apoptosis regulating proteins Bcl 2 and Bax . ^^^ MATERIAL AND METHODS : The immunoreactivity of Bcl 2 , Bax and ICH 1L were compared in ALS and control spinal cord motor neurons by immunohistochemical analysis and motor neurons with DNA fragmentation were identified by the TUNEL method . ^^^ RESULTS : The results demonstrate an increased expression of Bax in the ALS material as compared to controls but no change in Bcl 2 and ICH 1L expressions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of Akt kinase inhibits apoptosis and changes in Bcl 2 and Bax expression induced by nitric oxide in primary hippocampal neurons . ^^^ In addition , the P 13 kinase inhibitors blocked the effect of IGF 1 on down regulation in Bcl 2 and upregulation in Bax expression induced by NO . ^^^ Adenovirus mediated overexpression of the activated form of Akt significantly inhibited NO induced cell death , caspase 3 like activation , and changes in Bcl 2 and Bax expression . ^^^ These findings suggest that activation of Akt is necessary and sufficient for the effect of IGF 1 and is capable of preventing NO induced apoptosis by modulating the NO induced changes in Bcl 2 and Bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 , Bax , and Bak expression in short and long lived patients with diffuse large B cell lymphomas . ^^^ The expression of the apoptosis regulating proteins , Bcl 2 , Bcl 10 , Bax , and Bak , in the initial biopsy samples was examined with immunohistochemical methods . ^^^ An inverse association was found between length of patient survival and expression of Bcl 2 , Bcl 10 , and Bax . ^^^ Several combinations of protein expression , i . e . , Bcl 2 with Bax , Bcl 2 with Bcl 10 , and Bcl 10 with Bax , were different between the groups : a positive expression of these proteins was found in the short lived patients . ^^^ In diffuse large B cell NHL , Bcl 2 , Bcl 10 , and Bax expression alone or in combination is associated with chemoresistance and shortterm survival . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tamoxifen induced apoptosis in breast cancer cells relates to down regulation of bcl 2 , but not bax and bcl 10 ( L ) , without alteration of p 53 protein levels . ^^^ In this study , we investigated the effects of TAM on bcl 2 family gene products bcl 2 , bax , and bcl 10 ( L ) and on p 53 levels in estrogen receptor positive MCF 7 breast cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis regulating proteins bcl 2 , mcl 1 , and bax were visualized by immunohistochemistry . ^^^ No significant difference was found in the expression of bcl 2 , mcl 1 , and bax in BOOP and in UIP , suggesting that regulation of apoptosis might partly bypass the influence of these proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 also had no effect on the expression levels of proteins such as Fas , GADD 45 , Bax , Bcl 2 , Bcl 10 ( L ) or p 53 induced proteins ( PIGS ) in resting cells or after irradiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using the GM CSF dependent TF 1 myeloid leukaemia cell line , the authors show that the endogenous levels of BCL 2 and MCL 1 are downregulated upon GM CSF withdrawal , whereas the levels of BCL 10 ( L ) and Bax are unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , Bcl 10 , and Mcl 1 proteins and of the proliferation marker Ki 67 was evaluated by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 , p 21 ( waf / cip1 ) , c myc , bcl 2 , and bax was determined by western blot . ^^^ The protein level of p 53 , p 21 ( waf / cip1 ) , c myc , and bax was elevated while bcl 2 kept unchanged following drug exposure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fetal membranes also failed to exhibit significant immunoreactivity for BCL 2 but exhibited strong BAX immunoreactivity within the decidual layer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ginsenoside Rh 2 induces apoptosis independently of Bcl 2 , Bcl xL , or Bax in C6Bu 1 cells . ^^^ It was demonstrated that the expression of Bcl 2 , Bcl xL and Bax was not altered in ginsenoside Rh 2 treated C6Bu 1 cells . ^^^ These results suggest the existence of other apoptotic pathway that requires induction of apoptosis by ginsenoside Rh 2 rather than the pathway through Bcl 2 , Bcl xL or Bax in C6Bu 1 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Roles of p 53 , c Myc , Bcl 2 , Bax and caspases in serum deprivation induced neuronal apoptosis : a possible neuroprotective mechanism of basic fibroblast growth factor . ^^^ Using flow cytometric analysis , we examined the temporal changes of p 53 , c Myc , Bcl 2 , Bax expression in rat primary cortex neurons after serum deprivation . ^^^ Serum deprivation induced apoptosis accompanied by a rapid down regulation of p 53 , Bcl 2 and an up regulation of c Myc , Bax and caspase 3 activity . ^^^ Pretreatment with basic fibroblast growth factor prevented the apoptosis with an attenuation of the changes of p 53 , Bcl 2 , Bax levels and caspase 3 activity but had no effect on the change of c Myc level . ^^^ These results suggest that serum deprivation induces apoptosis through a signaling pathway involving p 53 , Bcl 2 , Bax , c Myc and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the antiapoptotic BCL 2 protein was decreased in diseased atria , whereas that of the proapoptotic BAX protein remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic cytosol facilitates Bax translocation to mitochondria that involves cytosolic factor regulated by Bcl 2 . ^^^ Proapoptotic members of the Bcl 2 family , including Bax , Bak , and Bid , directly trigger the mitochondrial release of apoptogenic cytochrome c and apoptosis inducing factor into the cytoplasm . ^^^ This apoptosis dependent promotion of Bax translocation was not seen with cytosol that was prepared from VP 16 treated cells expressing Bcl 2 . ^^^ Cytosol from cells with VP 16 induced apoptosis , but not that from normal cells or Bcl 2 expressing cells , induced cytochrome c release from isolated mitochondria , which , as assessed by immunodepletion experiments , was mainly mediated by Bax . ^^^ These results suggest that Bcl 2 exerts its antiapoptotic activity partly by inhibiting the translocation of Bax through the modification of cytosolic factors that are involved in such translocation during apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sodium butyrate also decreased the Bcl 2 level , whereas it increased the Bax level and stimulated the release of cytochrome c from the mitochondria , an event that was most likely responsible for the activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax content were evaluated either by flow cytometry after indirect immunolabelling or by Western blot . ^^^ The synthesis of Bcl 2 led to an increased Bcl 2 / Bax ratio solely in the cells with an apoptosis resistance phenotype . ^^^ They also confirm that the evolution of the Bcl 2 / Bax ratio reflects apoptotic ability better than the steady state level of Bcl 2 expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ratio of the expression of Bcl 2 to Bax or Bcl xL to Bax , and XIAP expression in synovial cells may not be directly associated with the susceptibility of synovial cells to apoptosis by LLL CHO . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bax and bcl xL expression in human sensitive and resistant leukemia cell lines . ^^^ Furthermore , the role of bcl 2 and bcl xL apoptosis inhibitors as well as bax expression ( apoptosis inducer ) in human sensitive leukemic cell lines ( CCRF CEM and HL 60 ) as compared to their resistant variants such as CCRF CEM / ACT400 , CCRF CEM / VCR1000 , HL 60 / IDA40 , HL 60 / DNR250 was evaluated . ^^^ Bcl 2 and bax were analyzed by both flow cytometry and ECL Western blot , bcl xL by ECL Western blot alone . ^^^ Comparison of the two sensitive cell lines demonstrated different bcl 2 , bax and bcl xL patterns . ^^^ The common characteristic of all resistant cell lines was the decreased expression of bax compared to bcl 2 or bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We determined the role of Bcl 2 family proteins Bcl 2 ( anti apoptotic ) and Bax ( pro apoptotic ) , activation of caspase 3 ( CPP32 / Yama ) and activation of PARP in CLL apoptosis . ^^^ All 21 analyzed CLL samples expressed Bcl 2 and Bax . ^^^ Four of 13 ( 31 % ) samples with a low Bcl 2 / Bax ratio exhibited in vitro prednisolone resistance , whereas eight of nine ( 88 % ) samples with a high Bcl 2 / Bax ratio were in vitro resistant ( < / =0 . 025 ) . ^^^ There was no significant correlation between clinical pre treatment status and Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax membrane insertion during Fas ( CD 95 ) induced apoptosis precedes cytochrome c release and is inhibited by Bcl 2 . ^^^ Overexpression of the anti apoptotic protein , Bcl 2 , prevented Bax insertion , cytochrome c release , complete processing of caspase 3 and 7 , and full activation of DEVD specific cleavage activity . ^^^ In addition , our findings indicate that prevention of Bax insertion into the mitochondria represents a novel mechanism by which Bcl 2 inhibits Fas induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The delay of neutrophil apoptosis was associated with markedly reduced levels of Bax , a pro apoptotic member of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , we characterized the regulation of antiapoptotic ( Bcl 2 , Bcl xL ) and proapoptotic ( Bad , Bax ) Bcl 2 family proteins in the rat heart during development and in oxidative stress induced apoptosis . ^^^ In unstimulated neonatal cardiac myocytes , Bcl 2 and Bcl xL were associated with the mitochondria , but Bad and Bax were predominantly present in a crude cytosolic fraction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis by S nitrosoglutathione and Cu2+ or Ni2+ ion through modulation of bax , bad , and bcl 2 proteins in human colon adenocarcinoma cells . ^^^ We also found that copper ions modulated the expression of bad , bax , and bcl 2 in GSNO treated HT 29 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis dysregulation is a major feature of CLL , and while no clear pattern has emerged , abnormal levels of bcl 2 are common in CLL and bcl 2 to bax ratios are also commonly disturbed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in oligodendrogliomas and their relationship to apoptosis . ^^^ The ratio of antagonists of apoptosis , such as Bcl 2 , to agonists , such as Bax , is thought to determine the outcome for a particular cell . ^^^ This study aimed to determine the expression of Bcl 2 and Bax proteins in a series of oligodendrogliomas and to relate the expression of these to measures of apoptosis . ^^^ Immunohistochemistry was used to detect the expression of Bcl 2 and Bax in an archival series of 32 oligodendrogliomas . ^^^ Bcl 2 and Bax were concluded to be expressed in a high proportion of oligodendrogliomas , suggesting that they may exert a regulatory role in cell death in these tumours . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the presence of starving medium alone or collagen 1 , the proapoptotic Bax was up regulated 2 2 . 5 fold , compared with soluble collagen 6 and fetal calf serum , whereas levels of the antiapoptotic Bcl 2 protein remained unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The differences of expression of BCL 2 , BCL 10 , BAX , and MCL 1 proteins of paired first resection and recurrence glioblastoma specimens were examined . ^^^ RESULTS : In the whole group , we found a significant up regulation of antiapoptotic BCL 2 ( median cumulative score of 15 in the primary , 19 at recurrence ; p < 0 . 0001 in the Wilcoxon test ) , BCLX ( median scores 20 and 25 , respectively , p < 0 . 0001 ) , and MCL 1 ( median scores 11 and 14 , p=0 . 0395 ) , and a significant down regulation of proapoptotic BAX ( median scores 14 and 11 , p < 0 . 0001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that Bcl 2 and Bax proteins were expressed in QGY 7703 cells . ^^^ At constant Bax protein levels , stable sense and antisense gene transfected QGY 7703 cells showed that constitutive expression of Bcl 2 could render the cells more resistant to Taxol and doxorubicin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumour proliferative rate ( evaluated as the ( 3 ) H thymidine labelling index , TLI ) , oestrogen and progesterone receptors ( ER and PgR , evaluated by the dextran coated charcoal method ) , nuclear DNA ploidy and the immunocytochemical expression of p 53 , bcl 2 and bax proteins were determined before primary treatment , at the time of diagnosis , and after primary chemotherapy , at surgery . ^^^ Such findings held true even within the sub set of patients who received adjuvant post operative chemotherapy , i . e . , those with node positive ( N ( + ) ) or ER ( ) / node negative ( N ( ) ) tumours , among whom also rapid proliferation or the presence of apoptosis favouring markers ( bcl 2 ( ) or bax ( + ) , singly and in association ) on surgical specimens identified a sub set of women who benefited from systemic treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The transformants were examined for the accumulation of p 53 , the induction of p21Waf1 / Cip1 / Sdi1 ( hereafter referred to as p 21 ) , and the other response of p 53 responsive genes ( MDM 2 , Bax , and Bcl 2 ) by Western blotting . ^^^ The 123A mutant did not induce p 21 , but normally responded to MDM 2 , Bax , and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the apoptosis related oncogenes bcl 2 , bax , and p 53 in Merkel cell carcinoma : can they predict treatment response and clinical outcome . ^^^ We used immunohistochemical methods to examine 25 Merkel cell carcinomas and 8 of their lymph node metastases to assess the status of the antiapoptotic gene bcl 2 and 2 proapoptotic genes , wild type p 53 and bax . ^^^ All tumors showed prominent bax immunopositivity ; 76 % were positive for bcl 2 , and only 28 % were positive for p 53 , the latter presumably reflecting mutated p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although the expression of the cell death promoting gene , bax , remained unchanged , a reduction of the bcl 2 / bax ratio , known to underlie the intracellular signal to apoptosis , was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Rat lung fibroblasts were treated with IL 1beta and / or TGF beta ( 1 ) and examined for expression of alpha SMA , iNOS , and the apoptotic regulatory proteins bax and bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bax , and Bcl 10 was not changed among Jurkat , Tax ( ) JPX 9 , and Tax ( + ) JPX 9 cells in the presence or absence of NF kappaB inhibitors . 10 chromosome linked inhibitor of apoptosis ( XIAP ) protein expression in Tax ( ) JPX 9 cells was significantly suppressed by NF kappaB inhibitors , however , its expression in Tax ( + ) JPX 9 cells was maintained even by the addition of NF kappaB inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Coincident with increased apoptotic susceptibility , WAS lymphocytes had markedly attenuated Bcl 2 expression , whereas Bax expression did not differ . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax : Bcl 2 ratio modulation by bryostatin 1 and novel antitubulin agents is important for susceptibility to drug induced apoptosis in the human early pre B acute lymphoblastic leukemia cell line , Reh . ^^^ The ratio of Bax to Bcl 2 protein can determine whether cells will die via apoptosis or be protected from it . ^^^ Reh was found to express a high basal level of Bcl 2 but was lacking of Bax protein expression . ^^^ Treatment with bryostatin 1 induced a down regulation in Bcl 2 protein that was not accompanied by an obvious Bax protein induction or apoptosis . ^^^ Western blots revealed that dolastatin 10 induced apoptosis was accompanied by the induction of Bax protein and the reduction in Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis in gestational trophoblastic disease is correlated with clinical outcome and Bcl 2 expression but not Bax expression . ^^^ In this study , we investigated apoptotic activity and the expression of two apoptosis regulatory genes , Bcl 2 and Bax , in an attempt to determine the role of apoptosis in GTD . ^^^ Expression of Bcl 2 and Bax were assessed immunohistochemically . ^^^ AIs of trophoblastic lesions in general inversely correlated with Bcl 2 expression ( P < . 001 ) , but no significant correlation was found between AI and Bax expression ( P > . 5 ) . ^^^ Bcl 2 expression is probably regulating apoptosis in normal placentas and GTD , whereas Bax expression is not . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In head injury , apoptosis has been recently implicated in many studies on animal brain samples the phenomenon of apoptotic gene expression ( bax and bcl 2 ) . ^^^ Immunohistochemical analysis was performed to detect the expression of bcl 2 , bax and p 53 using standard avidin biotin complex second antibody conjugate methodology utilising commercially available primary and secondary antibodies . ^^^ Our study showed that for the first time the strong expression of the pro apoptotic gene ( bax ) and low levels of the anti apoptotic gene ( bcl 2 ) , thus implicating the mechanism of apoptosis in brain injury following trauma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In apoptosis cascade , down regulation of Bcl 2 was seen at 12 hours and up regulation of Bax was seen at 24 hours , and caspase family ( caspase 3 / CPP32 , caspase 6 / Mch2 , and caspase 8 / FLICE protease ) activities peaked 72 hours after MNU treatment . ^^^ Therefore MNU induced photoreceptor cell death was attributed to DNA adduct formation restricted to photoreceptor cell nuclei leading to photoreceptor cell apoptosis by up regulation of Bax protein , down modulation of Bcl 2 protein , and activation of caspases 3 , 6 , and 8 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we analysed the expression of Bcl 2 and Bax , two proteins pivotal to the regulation of apoptosis , both immediately ex vivo and in viable and apoptotic sub populations following exposure to drug . ^^^ Our data suggest that high Bcl 2 / Bax ratios may be predictive of a drug resistant phenotype in B CLL cells and that modulation of these proteins is essential for the induction of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the yeast two hybrid system , Mcl 1 binds to the hypophosphorylated mutant of BAD and interacts preferentially with different proapoptotic ( Bax , Bak , Bok , Bik , and BOD ) compared with antiapoptotic Bcl 2 family members ( Bcl 2 , Bcl xL , Bcl w , Bfl 1 , CED 9 , and BHRF 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that neither p 53 nor Bcl 2 , Bcl XL and Bax protein expression changed during anoikis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 interaction in a transgenic mouse model of familial amyotrophic lateral sclerosis . ^^^ In asymptomatic transgenic mSOD 1 mice , expression of Bcl 2 , Bcl XL , Bad , and Bax does not differ from that in nontransgenic mice . ^^^ In contrast , in symptomatic mice , expression of Bcl 2 and Bcl XL , which inhibit apoptosis , is reduced , whereas expression of Bad and Bax , which stimulate apoptosis , is increased . ^^^ Relevant to the neuroprotective effects of Bcl 2 in transgenic mSOD 1 mice , overexpression of Bcl 2 increases the formation of Bcl 2 : Bax heterodimers , which abolish the Bax proapoptotic property . ^^^ That these changes contribute to the neurodegenerative process in this model of ALS is supported by our observations in double transgenic mSOD1 / Bcl 2 mice in which the pernicious increase of Bax is tempered by an increase in formation of Bcl 2 : Bax heterodimers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , the levels of p 21 and Bax protein were increased by ceramide in another hepatocarcinoma cell line , SK Hep 1 , while the Bcl 2 protein level was not changed . ^^^ Overexpression of Bcl 2 not only suppressed apoptosis but also completely prevented induction of p 21 and Bax caused by ceramide in SK Hep 1 cells . ^^^ Furthermore , overexpression of p 21 antagonized the death protective function of Bcl 2 and upregulated expression of Bax protein . ^^^ These results suggest that p 21 promotes ceramide induced apoptosis by enhancing the expression of Bax , thereby modulating the molecular ratio of Bcl 2 : Bax in human hepatocarcinoma cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism by which FDC spare malignant B cells from apoptosis did not involve alterations in levels of Bcl 2 , Bcl XL , or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of apoptosis regulating proteins bcl 2 , mcl 1 , bcl 10 , and bax in malignant mesothelioma . ^^^ We investigated apoptosis and the expression of bcl 2 , mcl 1 , bcl 10 , and bax in histological sections from 35 malignant mesotheliomas and 21 metastatic adenocarcinomas . ^^^ Moreover , the expression of bcl 2 , mcl 1 , bcl 10 , and bax were assessed by Western blotting in nonmalignant human mesothelial cells ( Met5A ) and seven malignant cell lines . ^^^ Patients with mesotheliomas showing a high apoptotic index ( > or =0 . 75 % ) had a worse prognosis ( P = 0 . 008 ) . bcl 2 positivity was observed in only seven cases , but bcl 10 , mcl 1 , and bax positivity was seen in all of them . ^^^ In immunoblotting experiments , all mesothelioma cell lines were negative for bcl 2 but positive for bcl 10 , mcl 1 , and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Age related hippocampal changes in Bcl 2 : Bax ratio , oxidative stress , redox active iron and apoptosis associated with aluminum induced neurodegeneration : increased susceptibility with aging . ^^^ In aged animals there is an initially intense immunopositivity at 3 hours for Bcl 2 , with negative staining for Bax . ^^^ By 72 hours , when apoptosis is strongly evident , Bcl 2 is negative and Bax strongly positive . ^^^ In contrast to the aged rabbits , young animals treated similarly with aluminum exhibit much less oxidative stress with no apoptosis , and maintain Bcl 2 immunopositivity and negative Bax staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We evaluated the role of the death genes p 53 , Bax , and Fas L ( ligand ) , survival gene Bcl 2 , interleukin converting enzyme ( ICE ) , and caspase 3 . ^^^ CsA induced the expression of p 53 ( P < 0 . 05 ) and Bax ( P < 0 . 01 ) and decreased that of Bcl 2 ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The extent of apoptosis and the immunohistochemical expression of bcl 2 and bax was also studied . ^^^ Strong bcl 2 expression was seen in 35 / 80 ( 44 % ) and strong bax expression in 20 / 80 ( 25 % ) lymphomas . ^^^ No significant association was found between the expression of bcl 2 or bax and the expression of the caspases . ^^^ Finally , the results suggest that the expression of bcl 2 or bax does not influence the expression of caspases 3 , 6 and 8 in malignant lymphomas to a significant degree . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The destruction of epithelial cells of Sjgren ' s syndrome patients is probably due to activation of several apoptotic pathways since epithelial cells express different apoptosis related molecules such as Fas , FasL , Bax , while mononuclear cells express Bcl 2 , Perforin and Granzymes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the pro apoptotic protein Bax and of the anti apoptotic protein Bcl 2 remained unchanged in MCF 7 and BT 20 cells upon GTN treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Clinical significance of CD 95 , Bcl 2 and Bax expression and CD 95 function in adult de novo acute myeloid leukemia in context of P glycoprotein function , maturation stage , and cytogenetics . ^^^ To study apoptosis regulating factors in acute myeloid leukemia ( AML ) , we investigated cell samples of adults with de novo AML by flow cytometry for constitutive expression levels of the apoptosis related molecules CD 95 ( n = 135 ) , Bcl 2 ( n = 131 ) , and Bax ( n = 66 ) , as well as spontaneous apoptosis in vitro ( n = 104 ) and susceptibility to anti CD 95 induced apoptosis ( CD 95 sensitivity ) ( n = 93 ) . ^^^ In stepwise logistic regression analyses , P gp function and the extent of spontaneous apoptosis in vitro as well as CD 95 sensitivity but not Bcl 2 , CD 95 , Bax , and CD 34 expression levels emerged as significant markers for response to induction chemotherapy . ^^^ We conclude that the constitutive expression of CD 95 and Bcl 2 , as well as CD 95 sensitivity and P gp function but not constitutive Bax expression depend on the maturation stage of leukemic cells in adult de novo AML . ^^^ P gp function , the extent of spontaneous apoptosis in vitro and CD 95 sensitivity are more predictive for response to induction chemotherapy in adult de novoAML than the constitutive expression levels of the apoptosis related molecules CD 95 , Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Damage induced apoptosis in intestinal epithelia from bcl 2 null and bax null mice : investigations of the mechanistic determinants of epithelial apoptosis in vivo . ^^^ The influence of bcl 2 and bax expression on apoptotic cell death in mouse intestinal epithelia was assessed using homozygously null mice . ^^^ These findings show that bcl 2 plays a key role in determining the sensitivity of colonic stem cells to damage induced death but that bax is not responsible for the p 53 dependent induction of apoptosis in this context . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These pre apoptotic events were associated with the cleavage of the caspase 3 , while the expression of Bcl 2 , Bcl XL and Bax proteins was not affected by the presence of Tat . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , bcl 10 ( s ) does not possess the 5th and 6th alpha helices ( thought to be the membrane spanning domains in bcl 2 , bcl 10 ( L ) , and bax ) and , therefore , should not be able to form membrane channels , thus eliminating this possible mechanism of action . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , and c Fos expression correlates to RPE cell apoptosis induced by UV light and daunorubicin . ^^^ The aim of this study was to determine the role of Bcl 2 , Bcl 10 L , Bax , and c Fos in regulation of apoptosis , induced by ultraviolet light A ( UV A ) and daunorubicin ( DNR ) , in retinal pigment epithelium ( RPE ) cells grown on bovine extracellular matrix ( ECM ) coated or uncoated plastic dishes . ^^^ Cellular expression of Bcl 2 , Bcl 10 L , Bax , and c Fos was determined by the use of antibodies and flow cytometry , Western blot analysis , and immunocytochemical staining . ^^^ RPE cells grown on ECM coated dishes expressed higher Bcl 2 levels and lower Bax levels compared to cells grown on uncoated dishes . ^^^ After UV A or DNR treatment , Bcl 2 , Bcl 10 L , Bax , and c Fos levels were differently regulated in cells grown on ECM coated dishes compared to cells grown on uncoated dishes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All TUNEL positive cells expressed the proapoptotic gene Bax , and 69 . 6+ / 4 . 6 % of the TUNEL positive cells expressed the antiapoptotic gene Bcl 2 ; the Bax expression was stronger than the Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the current study , the Lewis to F 344 rat cardiac allograft model was utilized as a means to immunohistochemically evaluate the expression of Bax , Bcl 2 , and factor 8 related antigen in transplant vascular disease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic status in prostate tumors was evaluated in situ , using the transferase deoxyuridine end labeling ( TUNEL ) assay , and the expression profile of two apoptotic proteins , bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 protein expression following radiation therapy versus radiation plus thermoradiotherapy in stage IIIB cervical carcinoma . ^^^ BACKGROUND : The relative amounts of Bcl 2 and Bax proteins determine cell survival or death following an apoptotic stimulus . ^^^ To clarify the molecular mechanism of cell death after radiotherapy or thermoradiotherapy and its relation to the response of AJCC / UICC Stage IIIB cervical carcinomas , the expression of Bax and Bcl 2 proteins was investigated both before and in the course of treatment given during this study . ^^^ The tumor sections were stained with anti Bax and anti Bcl 2 monoclonal antibody . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results demonstrate that Fas antigen , Fas ligand , c myb , c myc , bcl 2 , Bax and p 53 mRNAs are present in lacrimal tissues of F 1 , NOD , C3H , BALB / c , Tabby and Tfm mice . ^^^ Testosterone administration induced a significant increase in the lacrimal gland content of Bax mRNA , but a striking decrease in the lacrimal tissue level of bcl 2 mRNA in F 1 and C3H mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated how survival signals in the cellular microenvironment affect the expression , protein conformation , and protein protein interactions of the Bcl 2 family proteins Bax and Bcl 10 ( L ) and how changes in response to microenvironmental signals alter the response of cancer cells to the drug etoposide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , using yeast two hybrid and co immunoprecipitation studies , we show that RAD 9 , a human protein involved in the control of a cell cycle checkpoint , interacts with the anti apoptotic Bcl 2 family proteins BCL 2 and BCL 10 L , but not with the pro apoptotic BAX and BAD . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , an additional 18 kDa Bax related protein was expressed by the stimulation of G CSF or IL 6 , while Bcl 2 and Bcl 10 proteins remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mice were irradiated with a single dose ( 2 . 5 kJ / m ( 2 ) ) of UV from Kodacel filtered ( 290 400 nm ) FS 40 sunlamps and the skin tissues were analyzed at various times after irradiation for the presence of apoptotic cells and expression of p 53 , p 21 ( Waf 1 / Cip1 ) , bcl 2 , bax , and proliferating cell nuclear antigen . ^^^ In contrast , UV radiation induced high levels of bax at 24 to 72 hours after irradiation with a concomitant decrease in bcl 2 expression . ^^^ These results suggest that UV irradiation of mouse skin induces apoptosis mediated by the p53 / p21 / bax / bcl 2 pathway and that the dead cells are replaced by hyperproliferative cells , leading to epidermal hyperplasia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We assessed apoptosis by propidium iodide staining , by TUNEL nitrite production ( Griess reaction ) , by inducible nitric oxide synthase ( iNOS ) and Bcl 2 and Bax by Western blot . ^^^ A high rate of apoptosis was also associated with a decrease in the Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alteration of the Bcl 2 / Bax status of head and neck cancer cell lines by chemotherapeutic agents . ^^^ Among these genes , Bcl 2 and Bax control cell death , thus contributing to both tumor growth and drug sensitivity . ^^^ Then using four cell lines , which were KB ( Bcl 2+ / Bax+ ) , KCC L 871 ( Bcl 2+ / Bax ) , YCU T 891 ( Bcl 2 / Bax+ ) and TC 901 ( Bcl 2 / Bax ) , we investigated the impact of Bcl 2 / Bax status on sensitivity to the following chemotherapeutic agents ; paclitaxel , cisplatin , vincristine and 5 fluorouracil . ^^^ Immunohistochemistry and RT PCR showed that 71 % of the cell lines were Bcl 2 positive , 62 % were Bax positive , 38 % were Bcl XL positive and 62 % were Bcl XS positive . ^^^ In conclusion , Bcl 2 / Bax status was correlated with drug sensitivity and treatment with chemotherapeutic agents induced apoptosis in these cancer cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alterations in the expression of p 53 , bcl 2 and bax in the PKC isoenzyme overexpressing cells indicate that these proteins may be involved in the different sensitivities of these cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Flow cytometric analysis of HL 60 cells exposed to 2 AP showed appearance of a distinct peak representing the subdiploid cell population . 2 AP treatment decreased the ratio of anti apoptotic Bcl 2 to the death stimulating protein Bax , which may account for the molecular basis of apoptosis inducing activity of this chemopreventive organosulfur derivative . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and Bax in EGFR antisense transfected and untransfected glioblastoma cells . ^^^ The expression of bcl 2 and Bax in EGFR antisense transfected and un transfected glioblastoma cell line , U87E and U87V was studied by immunohistochemistry and western blotting . ^^^ Our results show that the expression of Bax was stronger and bcl 2 was weaker in EGFR antisense transfected cell line than the untransfected control . ^^^ Bcl 2 and Bax genes are probably involved in the reduction of malignancy of glioblastoma cell caused by the introduction of EGFR antisense into these tumor cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cartilage hair hypoplasia syndrome : increased apoptosis of T lymphocytes is associated with altered expression of Fas ( CD 95 ) , FasL ( CD95L ) , IAP , Bax , and Bcl 2 . ^^^ Increased apoptosis in CHH was associated with increased expression of Fas ( CD 95 ) , CD95L , and Bax and decreased expression of Bcl 2 and inhibitor of apoptosis protein ( IAP ) compared to the control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show that in B CLL cells levels of the death suppressor Bcl 2 correlated positively with those of the pro apoptotic protein Bax and of the cyclin dependent kinase ( cdk ) inhibitor p27Kip1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Through direct interaction with the voltage dependent anion channel ( VDAC ) , proapoptotic Bcl 2 family members such as Bax and Bak induce apoptogenic mitochondrial cytochrome c release and membrane potential ( Deltapsi ) loss in isolated mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was also accompanied by the up regulation of Bax , with modest down regulation of Bcl 2 protein expression , which changes the balance between pro and anti apoptosis molecules in favor of pro apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Two molecules belonging to the Bcl 2 family , Bcl 2 and Bcl 10 ( L ) , protect cells from Bax induced apoptosis and show distinct expression patterns in adult neurons , with downregulated Bcl 2 and highly upregulated Bcl 10 ( L ) expression . ^^^ To investigate the biological functions of these two molecules in Bax mediated apoptosis in neurons , we transduced various levels of Bcl 10 ( L ) or Bcl 2 via adenoviral vectors into nerve growth factor ( NGF ) treated PC 12 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mutant p 53 protein , Bcl 2 / Bax ratios and apoptosis in paediatric acute lymphoblastic leukaemia . ^^^ The anti apoptotic function of the Bcl 2 protein appears to be modulated by its ability to heterodimerize with other members of the gene family , predominantly Bax , a protein favouring induction of apoptosis . ^^^ Susceptibility to undergoing apoptosis may , therefore , be dependent on the ratio between Bcl 2 and Bax . ^^^ Both Bax and Bcl 2 are regulated by the tumour suppressor protein p 53 . ^^^ The present study therefore aims to study the significance of the Bcl 2 : Bax ratio , p 53 expression and apoptosis in paediatric acute lymphoblastic leukaemia ( ALL ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the proapoptotic protein bax and the antiapoptotic protein bcl 2 was determined with Western blot assay . ^^^ The increase in bax / bcl 2 ratio in DHA stimulated cells was not affected by ET 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Then we determined whether Bcl 2 , Bax , Bcl xL and Bcl xS , which regulate the release of cytochrome c from mitochondria , were altered in the ischemia tolerant CA 1 region . ^^^ Bcl 2 and Bax were up regulated in the ischemia tolerant group , but Bcl xL and Bcl xS showed no apparent difference in their expression . ^^^ These results suggest that cytochrome c release is prevented in CA 1 neurons in gerbils in which ischemia tolerance had been induced and that the altered ratio of Bcl 2 to Bax may play a part in this mechanism . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Knockout studies have shown that spontaneous apoptosis is independent of p 53 and Bax in both small and large intestine , whereas Bcl 2 only regulates spontaneous apoptosis in the colon . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This permeabilization event is at least in part under the control of the permeability transition pore complex ( PTPC ) , which interacts with oncoproteins from the Bcl 2 family as well as with tumor suppressor proteins from the Bax family , which inhibit or facilitate membrane permeabilization , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The predictive role of tumour proliferative rate and expression of p 53 , bcl 2 and bax proteins , alone and in association with tumour size , nodal involvement and oestrogen receptors ( ER ) , was analysed on 145 elderly patients ( > or =70 years of age ) with histologically assessed node positive breast cancers treated with radical or conservative surgery plus radiotherapy followed by adjuvant tamoxifen for at least 1 year . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the BCL 2 family proteins , BCL 2 , BAX , BCL ( XL ) and BAK have been determined in a panel of 12 human ovarian carcinoma cell lines encompassing a wide range in sensitivity to cisplatin . ^^^ Whereas BAX , BCL ( XL ) and BAK levels did not correlate with sensitivity , there was a statistically significant inverse correlation ( r = 0 . 81 ; P = 0 . 002 ) between growth inhibition by cisplatin and BCL 2 levels . ^^^ Two relatively sensitive lines ( A 2780 and CH 1 ) have been stably transfected with bcl 2 and bcl ( XL ) respectively and two relatively resistant lines ( A2780cisR and SKOV 3 ) stably transfected with bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of endogenous p 53 activity did not affect the sensitivity of TAD 2 cells to iodide , and Western blot analysis demonstrated that p 53 , Bcl 2 , Bcl XL , and Bax protein expression did not change when cells were treated with iodide . ^^^ This type of apoptosis is p 53 independent , does not require protein synthesis , and is not induced by modulation of Bcl 2 , Bcl XL , or Bax protein expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis resistant phenotype of A 11 cells was associated with the expression level of caspase 3 , but not with those of Bcl 2 , Bcl 10 ( L ) Bax , p27Kip1 and DAP kinase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because the apoptotic process is regulated by several oncoproteins , the expression of Bcl 2 and Bax was analyzed by immunohistochemistry . ^^^ CONCLUSION : These results suggest that hyperparathyroidism is associated with a compensatory increase in apoptosis , possibly favored by a diminished Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of Bcl 2 and Bax expression in relation to cell turnover and epithelial differentiation markers in the non lactating human mammary gland epithelium . ^^^ The expression of the apoptosis related proteins Bcl 2 and Bax was investigated by immunohistochemistry in the normal non lactating human mammary gland in relation to cell proliferation and apoptosis . ^^^ In order to characterize individual Bax / Bcl 2 immunoreactive cells , the epithelial markers cytokeratin 14 and 19 and the macrophage marker CD 68 were used . ^^^ Weak immunoreactivity for Bcl 2 and Bax was present throughout all epithelia , suggesting a balance between pro and antiapoptotic effects in the majority of epithelial cells . ^^^ However , specific cells showed a strong staining for Bax or Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : 15 specimens of pterygia from 15 eyes were examined , together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis ( p 53 , bcl 2 , and bax ) . ^^^ These cells were shown to express significant levels of p 53 and bax , as well as the apoptosis inhibiting protein bcl 2 . ^^^ In contrast , normal conjunctival specimens displayed no bcl 2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer , coupled with high levels of bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Influence of age on hypoxia / reoxygenation induced DNA fragmentation and bcl 2 , bcl xl , bax and fas in the rat heart and brain . ^^^ The ratios of bcl 2 / bax and of bcl xL / bax were higher in the old heart and brain compared to that in the young adult after hypoxia / reoxygenation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also found modest up regulation of Bax and down regulation of Bcl 2 , but there was a significant down regulation of c erbB 2 in flavopiridol treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Compatible with the more efficient induction of apoptosis , Adp 53 , but not Adp 21 , induced a marked increase in expression of the preapoptotic molecule BAX without a concomitant change in expression of the antiapoptotic mediator Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax regulate the channel activity of the mitochondrial adenine nucleotide translocator . ^^^ Bcl 2 family protein including anti apoptotic ( Bcl 2 ) or pro apoptotic ( Bax ) members can form ion channels when incorporated into synthetic lipid bilayers . ^^^ In contrast to the combination of Bax and ANT , ANT does not form active channels when incorporated into membranes with Bcl 2 . ^^^ Bcl 2 prevents channel formation by Atr treated ANT and neutralizes the cooperation between Bax and ANT . ^^^ Our data are compatible with a mnage  trois model of mitochondrial apoptosis regulation in which ANT , the likely pore forming protein within the PTPC , interacts with Bax or Bcl 2 which influence its pore forming potential in opposing manners . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study hypothesized that apoptosis regulatory genes other than Fas Fas ligand , such as p 53 , p 21 ( Waf1 / Cip1 ) , bcl 2 , bcl 10 , and bax , may also participate in epithelial cell apoptosis in this model . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The overexpression of the genes directly ( B cell leukemia / lymphoma 2 , BCL 2 ) or indirectly ( multidrug resistance gene 1 , MDR 1 ) involved in the suppression of apoptosis , as well as the low level expression of their antagonists ( BCL 2 associated 10 protein , BAX ) result in longer survival and the development of drug resistance in malignant cells . ^^^ AIM : The objective of this study was to design our own appropriate primers and to synthesize internal RNA standards and this way to evaluate the rate of expression of the MDR 1 , BCL 2 and BAX genes using a competitive reverse transcription and polymerase chain reaction ( cRT PCR ) . ^^^ The measured band ' s intensity of light and the calculated standard / patient ratio were employed in the analysis of the MDR 1 , BCL 2 and BAX gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of Bcl 2 family proteins ( Bax , Bcl 2 and Bcl 10 ) on cellular susceptibility to radiation in pancreatic cancer cells . ^^^ The aim of this study was to examine Bax , Bcl 2 and Bcl XL proteins in human pancreatic cancer cell lines and to clarify the mechanism of radiation resistance . ^^^ Western blotting showed that the basal level of the Bax / Bcl 2 ratio reflected the radiosensitivity of these cell lines , and Bax expression was obviously upregulated after irradiation in the presence of mutated p 53 , but Bcl 2 expression remained almost constant . ^^^ Both PANC 1 / Rad and AsPC 1 / Rad cells had greater Bcl XL expression than the parental cells , and the basal level of the Bax / Bcl 2 ratio was no longer predictive of radiosensitivity . ^^^ Upregulated expression of Bax protein after irradiation was not related to induction of apoptosis in these cells , suggesting that overexpression of Bcl XL and functional reconstruction of Bcl 2 family proteins are important factors in acquired radioresistance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD95L , CD 95 , apoptosis induced cysteine protease CPP 32 , apoptosis promoting protein Bax , and proliferating marker ( MIB 1 ) frequently were positive in the lymphoma cells of cases with angiodestruction , but there was no expression of apoptosis inhibitor protein Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased expression of several apoptosis related genes ( p 53 , fas , ratio of bax to bcl 2 , GAPDH ) in DS brains has been reported . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis in gallbladder carcinomas and dysplasias , its relation to the expression of caspases 3 , 6 and 8 and apoptosis regulating proteins bcl 2 , mcl 1 and bax . ^^^ In this study we investigated apoptosis and the expression of caspases 3 , 6 and 8 and bcl 2 , mcl 1 and bax in 39 gallbladder carcinomas and 7 epithelial dysplasias . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a proapoptotic member of the Bcl 2 family of proteins which localizes to and uses mitochondria as its major site of action . ^^^ Bax normally resides in the cytoplasm and translocates to mitochondria in response to apoptotic stimuli , and it promotes apoptosis in two ways : ( 1 ) by disrupting mitochondrial membrane barrier function by formation of ion permeable pores in mitochondrial membranes and ( 2 ) by binding to antiapoptotic Bcl 2 family proteins via its BH 3 domain and inhibiting their functions . ^^^ These observations reveal new functions for the alpha 5 alpha6 hairpin loop of Bax : ( 1 ) regulation of mitochondrial targeting and ( 2 ) modulation of binding to antiapoptotic Bcl 2 proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results demonstrate that induction of anti apoptotic bcl 2 related proteins at an early stage of differentiation is important for the maintenance of HCC T cell differentiation by antagonizing pro apoptotic molecules such as Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , and other members of the family , such as Bax , are located downstream to p 53 in the apoptotic pathway , and they contain negative or positive p 53 response elements . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , an apoptosis inhibitor , and Bax , an apoptosis promoter , show tightly regulated , hair cycle dependent expression patterns : during catagen , the distal ORS of the HF remains strongly positive for Bcl 2 and Bax ; in contrast , the proximal epithelial part of the HF loses most Bcl 2 expression while it remains strongly positive for Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In an effort to understand the molecular basis of lymphomagenesis in RARalpha deficient transgenic mice , we compared the levels of RARalpha to the levels of p 53 mRNA , and Bcl 2 , and Bax proteins in lymphoid and non lymphoid tissues and in lymphomas derived from the RARalpha deficient transgenic mice . ^^^ Bcl 2 to Bax ratios were highly increased in the lymphoid compartments ( spleen > bone marrow > thymus ) because of selective overexpression of Bcl 2 protein . ^^^ In summary , RARalpha downmodulation in this transgenic mouse model system was accompanied by p 53 downmodulation and deregulation of Bcl 2 to Bax ratios in the lymphoid compartments . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of p 53 altered the expression of Fas antigen ( Fas R ) , Bax and Bcl 2 ; however , it remains unclear how the loss of p 53 modifies the expression of these molecules . ^^^ The apoptosis related molecules examined were the Fas R , Bcl 2 , Bax , and Fas associated phosphatase 1 ( FAP 1 ) . ^^^ These data demonstrate that both the loss of p 53 and carcinogen stimulation are associated with altered expression of Fas R , Bcl 2 and FAP 1 , although the loss of p 53 is sufficient for altered expression of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the bcl 2 and bax oncoprotein in TCC and its clinical significances ] . ^^^ To investigate the role of bcl 2 ( an apoptosis suppressing oncogene ) and bax ( an apoptosis accelerating oncogene ) in the development of TCC . ^^^ We investigated bcl 2 and bax expression by means of immunohistochemical technique in 34 cases of TCC and in 9 cases of normal bladder tissue . ^^^ These results suggested that increased expression of bcl 2 and decreased expression of bax in TCC play an important role in the development of TCC . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To study the protective effect of melatonin against neuronal injury and the possible roles of alteration in the expression of bcl 2 and bax following brain ischemia . ^^^ The expression of bcl 2 and bax was analyzed by immunohistochemical method . ^^^ Melatonin given before the ischemia enhanced the expression of bcl 2 in the penumbra area and had no significant effect on the expression of bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatments caused PCD ( light microscopy and TUNEL assay ) with altered mRNA expression ( RT PCR ) of bax ( pro apoptotic ) and bcl 2 ( anti apoptotic ) genes , resulting in a high bax / bcl 2 ratio . ^^^ Pretreatment of cells with MDL 28170 ( a cell permeable and selective inhibitor of calpain ) prevented increase in bax / bcl 2 ratio , upregulation of calpain , degradation of 68 kDa NFP , and occurrence of PCD . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 : bax and bcl 2 : Bcl 10 ratios by image cytometric quantitation of immunohistochemical expression in ovarian carcinoma : correlation with prognosis . ^^^ Bax and bcl 10 are members of the bcl 2 family ; when overexpressed , they can counteract the ability of bcl 2 to inhibit apoptosis . ^^^ This suggests a model in which the ratios of bcl 2 to bax and bcl 10 can be used to determine response to therapy and prognosis . ^^^ The expression of bcl 2 , bax and bcl 10 was studied in 50 ovarian carcinomas . ^^^ The ratios were obtained by dividing the PPA of bcl 2 by the PPA of bax and bcl 10 . 17 of 50 ovarian carcinomas ( 34 % ) stained positively for bcl 2 , 39 for bax ( 78 % ) and 47 for bcl 10 ( 94 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined immunohistochemically 4 apoptosis regulating bcl 2 familial proteins ( bcl 2 , mcl 1 , bcl 10 , and bax ) in the biliary epithelium in 19 cases of primary biliary cirrhosis . ^^^ Bcl 2 and mcl 1 are inhibitors of apoptosis , bcl 10 , probably bcl XL in biliary epithelial cells , an inhibitor , and bax , a promoter of apoptosis . ^^^ Expression levels of mcl 1 , bcl 10 , and bax were similarly reduced to that of bcl 2 in these 2 diseases . ^^^ These findings suggest that bax is not important as a proapoptotic factor in the damaged bile ducts and that downregulation of bcl 2 and mcl 1 , and probably that of bcl XL , leads to a decrease in the threshold of apoptosis and increase in the vulnerability to apoptotic stimuli in these bile ducts , followed by the progressive apoptotic loss of interlobular bile ducts , in primary biliary cirrhosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To define apoptotic chondrocyte death and the expression of Bcl 2 , Bax , and Fas in human osteoarthritis ( OA ) cartilage . ^^^ Immunohistochemistry was done to study the expression of Bcl 2 , Bax , and Fas . ^^^ Apoptotic cells and Bcl 2 , Bax , and Fas positive cells were counted within defined microscopic fields . ^^^ Expression of Bcl 2 and Bax was verified by Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Elucidation of the signal transduction mechanism revealed involvement of protein tyrosine kinase , protein kinase C and ser / thr phosphatases with concomitant increase in the level of protein products of apoptosis associated genes p 53 , bax , bad , fas and fas ligand and cleavage of N terminal 23 kDa fragment of Bcl 2 that exhibited Bax like death effector properties . ^^^ The signal transduction cascade evoked by thymosin alpha 1 involves activation of protein kinase C with a decrease in the level of protein products of proapoptotic genes like bax and bad and increase in the protein products of bcl 2 gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , an increased expression of Bax and decreased expression of Bcl 2 ( both at the protein and mRNA level ) was found in lymphocytes from aged subjects . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and BAX expression in hyperplastic and dysplastic rectal polyps . ^^^ BACKGROUND / AIMS : Members of the gene family that includes BCL 2 and BAX are functionally antagonists in the apoptosis process and they have been observed in normal and neoplastic tissues . ^^^ The aim of this study is to investigate the combined effects of BCL 2 and BAX protein in normal mucosa , dysplastic and hyperplastic polyps of the rectum . ^^^ METHODOLOGY : We studied BCL 2 and BAX protein expression in 40 cases of adenomatous polyps all located in the rectum , with different dysplastic gradings , and the mean time in 10 cases of normal rectal mucosa . ^^^ CONCLUSIONS : These preliminary data suggest that BCL 2 and BAX confirm a probably different role in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the effect of mutant p 53 on camptothecin induced apoptosis and the expression of genes which are known to be involved in apoptosis or drug resistance , such as bcl 2 , bax , bak , p21waf1 , and P glycoprotein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No prognostic impact was shown in relation to the expression of p 53 and the apoptosis regulating molecules Bcl 2 and Bax . ^^^ In the multimodally treated esophageal cancer patients , strong expression of p21WAF1 and accumulation of p 53 were predictors of poor survival , whereas expression of Bcl 2 , Bax , and Bcl XL had no prognostic significance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In an effort to understand this role , we have used polyomavirus transformed pyF 111 rat fibroblasts , which are hypersusceptible to apoptosis as they constitutively hyperexpress PKC delta , but can not make the antiapoptotic Bcl 2 and Bcl 10 ( L ) proteins , while making the proapoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Results from Western blot analysis indicated that HepG 2 cells did not express either the death repressor Bcl 2 , or the death promoters Bcl XS and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Phenylbutyrate induced apoptosis and differential expression of Bcl 2 , Bax , p 53 and Fas in human prostate cancer cell lines . ^^^ STUDY DESIGN : We analyzed the differential expression of different apoptosis modulators , Bcl 2 , Bax , p 53 and Fas , for their potential role in PB induced apoptosis using relative quantitative flow cytometry ( FCM ) . ^^^ Bcl 2 was down regulated 30 75 % and the Bax : Bcl 2 ratio elevated in apoptotic PCa cell lines regardless of their androgen dependency or p 53 status . ^^^ FCM revealed a heterogeneous stimulatory effect on the expression of Bax and Bcl 2 in PC 3 PF cells at 0 . 5 2 . 5 mM PB . ^^^ CONCLUSION : Our data show that PB induced PCa apoptosis is associated with the relative repression of Bcl 2 and with up regulation of Bax and Fas proteins and that this PB induced apoptosis is independent of p 53 and androgen dependency status of PCa cell lines . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of BCL 2 , BCL 10 , and BAX in primary central nervous system lymphomas that occur in immunosuppressed patients . ^^^ The BCL 2 family proteins ( BCL 2 , BCL 10 , MCL 1 , and BAX ) and p 53 expression were studied by immunohistochemistry on paraffin slides . ^^^ In contrast , PCNSLs in immunosuppressed patients were shown to express high levels of BCL 2 , BCL 10 , and BAX in more than 80 % of tumor cells in 7 , 10 , and 11 cases , respectively . ^^^ In immunocompetent patients , only one case showed a high level of BCL 2 expression in more than 80 % of the cells , whereas BCL 10 and BAX were overexpressed in two cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax proteins are nuclear matrix associated proteins . ^^^ Bcl 2 and Bax proteins are implicated in the regulation of apoptosis . ^^^ The association of bcl 2 and Bax protein with nuclear matrix in glioblastoma cell line U 343 was studied by confocal microscopy and Western blotting . ^^^ Confocal microscopic images display that bcl 2 was localized at the peripheral of the nuclear matrix and Bax protein was located in the nuclear matrix . ^^^ Western blotting detected a 26 kDa bcl 2 band and a specific band of Bax at around 66 kDa in nuclear matrix proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have found that whereas TP 53 , BAK , ICH 1 Short form , and DAD 1 were expressed at constant levels , WAF 1 , PCNA , BAX were up regulated , ICH 1 Long form , DNA LIGASE 1 , and BCL 2 were down regulated . ^^^ A positive correlation of WAF 1 and BAX , and a borderline negative correlation with BCL 2 expressions were observed with micronuclei frequency for doses ranging from 0 . 5 to 4 Gy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study analyzes the temporal and spatial relationship between expression of pro and anti apoptotic members of the Bcl 2 gene family ( Bcl 2 , Bcl 10 ( L ) , Bax ) and epidermal growth factor ( EGF ) , insulin like growth factor ( IGF 1 ) , and transforming growth factor beta ( TGF beta ) , growth factors that are thought to be reparative in ARF . ^^^ In control kidneys , expression of Bcl 2 , Bcl 10 ( L ) was low in epithelium of distal tubules , Bax had low to moderate expression in the proximal tubule and had low expression in the distal tubule , EGF and IGF 1 had low to moderate expression in the distal tubule , and TGF beta had low expression in the proximal tubule . ^^^ In contrast , within 24 h of reperfusion , distal tubules showed a marked increase in expression of Bcl 2 and a moderate increase in Bcl 10 ( L ) and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , Bcl 10 ( L ) and Bcl 10 ( S ) expression in neoplastic and normal endometrium . ^^^ The two anti apoptotic proteins Bcl 2 and Bcl 10 ( L ) , and the two pro apoptotic proteins Bax and Bcl 10 ( S ) were measured by Western blotting in 51 neoplastic and 8 normal endometrial samples . ^^^ Despite the fact that the amounts of Bcl 2 , Bax and Bcl 10 ( L ) proteins in the neoplastic population were not significantly differently distributed according to the clinicopathological features of the patients , the differences observed between normal and neoplastic samples suggest that these proteins may play a role in endometrial carcinoma : long term follow up studies will be required to confirm this hypothesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We tend to give acquired findings into the correlation with expression of proteins Bcl 2 and Bax ( products of genes regulating apoptosis ) . ^^^ The level of expression of Bcl 2 and Bax was determined using standard three step immunohistochemical procedure . ^^^ Results were achieved by the comparison of apoptoic index and the level expression of Bcl 2 and Bax was semiquantitatively evaluated . ^^^ The low value of apoptotic index was mostly accompanied by the high expression of Bcl 2 and the Bax expression was not proportionally related to the value of apoptic index . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunofluorescence did reveal an increased nuclear translocation of p 53 suggesting activation of the protein , but such a translocation did not lead to significant changes in either the expression of the cyclin dependent kinase inhibitor , p 21 ( WAF1 / CIP1 ) the cell survival factor Bcl 2 or the pro apoptotic factor Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Other apoptosis related molecules , p 21 , Bcl 2 and Bax , that are in the cascade of p 53 pathway , were also examined . p 53 was expressed in cells residing in the vicinity of transection as early as 30 min . ^^^ In view of the results of the double staining of p 53 and Bcl 2 , Bax or TUNEL , a variety of apoptosis related molecules are expressed with p 53 , all within the first three days of injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immunohistochemical staining showed that the number of bcl 2 protein positive cells decreased , but the number of bax protein positive cells increased . ^^^ The mechanism of arsenic trioxide may mainly be inducing liver cancer cells to undergo apoptosis , which may be related to downregulate the expression of bcl 2 genes and upregulate the expression of bax genes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A significant increase and reduction in the number of bax and bcl 2 positive cells , respectively , following cisplatin treatment was observed in the cells of the organ of Corti , the spiral ganglion , and the lateral wall . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 inhibits Bax translocation from cytosol to mitochondria during drug induced apoptosis of human tumor cells . ^^^ High level overexpression of the anti apoptotic protein Bcl 2 prevented Bax redistribution to the mitochondria , caspase activation and apoptosis following exposure to staurosporine or etoposide . ^^^ These data confirm the role of Bax in mitochondrial cytochrome c release , and indicate that prevention of Bax translocation to the mitochondrial membrane represents a novel mechanism by which Bcl 2 inhibits drug induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No prognostic impact could be shown in relation to the expression of the proliferation regulating molecule p 53 and the apoptosis regulating molecules Bcl 2 and Bax . ^^^ Among multimodally treated esophageal cancer patients , again strong expression of p21WAF1 as well as accumulation of p 53 were predictors of poor survival , whereas expression of Bcl 2 , Bax and Bcl XL did not show any prognostic influence . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of tissue samples resulted in marked differences of bcl 2 and bax protein expressions during involution and between groups . ^^^ The bax expression was decreased by alpha tocopherol deprivation at 1 , 3 , and 5 d , but not at 8 d , while the bcl 2 score was higher only at 8 d ( 1 . 5 vs . 0 . 0 for experimental and control groups , respectively ) . ^^^ As a result , the bcl 2 to bax ratios were increased for the experimental group at 1 and 8 d . ^^^ During involution , apoptotic counts increased ( from 0 . 12 to 4 . 06 % ) , but no effects were detected in relation to bcl 2 to bax ratio and alpha tocopherol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family proteins govern this mitochondria dependent apoptosis pathway , with proteins such as Bax functioning as inducers and proteins such as Bcl 2 and Bcl 10 ( L ) serving as suppressors of cell death . ^^^ The BAR protein contains a SAM domain , which is required for its interactions with Bcl 2 and Bcl 10 ( L ) and for suppression of Bax induced cell death in both mammalian cells and yeast . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate whether overexpression of Bax , an apoptosis promoting gene , sensitizes KATOIII gastric cancer cells to apoptosis induced by chemotherapeutic agents , three stable cell lines of KATOIII transfected with Bax ( KATOIII Bax ) , Bcl 2 ( KATOIII Bcl 2 ) , or control pCI neo expression vector ( KATOIII pCI neo ) were established . ^^^ Our results showed that the sensitivity of the KATOIII Bax cells to chemotherapeutic agents was enhanced compared with that of the KATOIII pCI neo cells , and the KATOIII Bcl 2 cells were more resistant to these agents . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dysregulated expression of bcl 2 and bax in oral carcinomas : evidence of post transcriptional control . ^^^ A study was conducted to investigate the gene expression of bcl 2 and bax in oral squamous cell carcinomas . ^^^ We used reverse transcriptase polymerase chain reaction ( RT PCR ) to evaluate the expression of bcl 2 and bax mRNAs and the ratio of bcl 2 / bax mRNA , and employed immunohistochemistry to investigate the bcl 2 and bax encoded proteins . ^^^ It was observed that the expression level of bcl 2 mRNA or bax mRNA was not consistent with their protein level in some cases . ^^^ The ratio of bcl 2 / bax mRNA was higher in carcinomas than in the adjacent histologically normal oral epithelium , and higher ratios were seen in most of poorly differentiated carcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , the levels of BCL 2 protein were not modulated by As2O3 , but it did upregulate BAX protein in UF 1 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the regulation of apoptosis , we measured caspase 3 and caspase 8 activity by ELISA , and examined the Bcl 2 and Bax protein level by Western blot . ^^^ Overexpression of 1 kappa B delta N augmented TNF alpha induced caspase 3 and caspase 8 activity , but did not affect Bcl 2 or Bax protein expression . ^^^ CONCLUSION : Overexpression of 1 kappa B delta N potentiates TNF alpha induced apoptosis and augments caspase 8 and caspase 3 activity in rat MCs without changing Bcl 2 or Bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHOD : The expression of genes that promote ( c myc , Bax , BclxS ) or protect ( Bcl 2 , BclxL ) from cell death was studied by Northern blot , Western blot , and immunohistochemistry in murine kidneys following ARF induced by folic acid or in renal tubular epithelial cells ( MCT ) stressed in culture . ^^^ RESULTS : Renal mRNA levels encoding for c myc and BclxL were elevated in ARF while the Bcl2 / Bax ratio was decreased ( Bcl 2 decreased and Bax increased ; P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we show that inhibition of the proteasome activity in Bcl 2 overexpressing cells accumulates the proapoptotic Bax protein to mitochondria / cytoplasm , where it interacts to Bcl 2 protein . ^^^ Our studies strongly suggest that ubiquitin / proteasome mediated Bax degradation is a novel survival mechanism in human cancer cells and that selective targeting of this pathway should provide a unique approach for treatment of human cancers , especially those overexpressing Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Doxazosin modifies Bcl 2 and Bax protein expression in the left ventricle of spontaneously hypertensive rats . ^^^ OBJECTIVE : To investigate the molecular basis of apoptosis in the left ventricle of SHRs in terms of the expression of Bcl 2 protein ( which protects from apoptosis ) and Bax protein ( which acts as an apoptotic promoter ) . ^^^ RESULTS : The left ventricles of untreated SHRs showed a significant increase in Bcl 2 protein expression and a reduced presence of Bax protein . ^^^ The ratio of Bcl 2 : Bax in SHRs was higher than in WKY rats , suggesting an anti apoptotic state . ^^^ Bax promotes cell apoptosis when it is bound to Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase 8 and 3 expression , as well as two anti apoptotic proteins Bcl 2 and HSP 70 , and one proapoptotic protein Bax were also investigated by immunoblot allowing identification of several apoptotic pathways based on the behaviour of the different studied proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of TGF beta 1 , an auto / paracrine antiproliferative and apoptogenic factor on Bax transcript level ( RT PCR ) , subcellular distribution of Bax protein ( immunoelectron microscopy ) , Bcl 2 protein level and apoptotic cell number ( flow cytometry with FITC conjugated monoclonal anti Bcl 2 antibody and DNA stained with DAPI ) in HC 11 mouse mammary epithelial cells was examined . ^^^ Prolactin protected HC 11 cells against TGF beta 1 induced PCD , which could occur at two levels : 1 ) TGF beta 1 expression , through the decrease of TGF beta 1 transcript content , and 2 ) Bax / Bcl 2 checkpoint , through down regulation of Bax and up regulation of Bcl 2 . ^^^ In conclusion , Bax and Bcl 2 proteins are implicated in the mechanism of TGF beta 1 induced PCD and antiapoptotic action of prolactin in HC 11 mouse mammary epithelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : The purpose of the present study was to investigate whether apoptosis is triggered during ischemia ( 1 ) and reperfusion ( R ) and whether I / R induced apoptosis is correlated with changes in expression of Bcl 2 and Bax . ^^^ Densitometrically , Western blot analysis showed significant reduction in expression of Bcl 2 ( 16 + / 1 % ) and increase in Bax ( 29 + / 8 % ) after 6 h R in the necrotic area compared with normal tissue while expression of these two proteins was not changed in the PI group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study tested the hypothesis that IPC reduces ischemia / reperfusion induced myocardial apoptosis by inhibiting neutrophil ( PMN ) accumulation and altering expression of Bcl 2 and Bax proteins . ^^^ PMN accumulation was detected immunohistochemically with anti rat CD 18 antibody ( WT 3 ) and expression of Bcl 2 and Bax proteins was analyzed using Western blot assay . ^^^ Densitometrically , IPC significantly attenuated the ischemia / reperfusion upregulated expression of Bax protein in the ischemic zone from 204 + / 57 % in the control group to 76 + / 7 % ( P < 0 . 05 ) , while the expression of Bcl 2 was not different from the non ischemic zone in either group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis during reperfusion was associated with the cleavage of caspase 3 , the final down stream executioner caspase , whereas the protein levels of the anti apoptotic protein Bcl 2 and the pro apoptotic protein Bax were unchanged . ^^^ Inhibition of NO synthesis thereby activates the caspase cascade , whereas the Bcl 2 / Bax protein levels remained unchanged . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fibroblasts and protein products of Bcl 2 and Bax were measured by quantitative immunohistochemistry . ^^^ Blood pressure ( BP ) in SHR started to increase significantly at age 5 weeks ; ( 3 ) fibroblasts were significantly increased in the heart of SHR ; ( 4 ) the ratio of Bcl 2 / Bax was significantly reduced in SHR ; and ( 6 ) ramipril effectively reduced apoptosis and fibroblasts , and increased the ratio of Bcl 2 / Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis could be induced in cell lines that overexpressed Bcl 2 or Bcl XL when the cells were treated with anti Fas antibody , tumor necrosis factor alpha , staurosporine , or Bax , in addition to TC A . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , whereas bcl 2 was not detected , we observed increased expression of the proapoptotic protein bax , the release of cytochrome c from the mitochondria to the cytosol , and the proteolytic cleavage of poly ( ADP ribose ) polymerase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The oncoprotein bcl 2 inhibits many forms of apoptosis , whereas the related protein bax promotes apoptosis . ^^^ To evaluate the interaction of bcl 2 , bax , and apoptosis in the renal response to UUO , adult and neonatal rats were subjected to UUO or sham operation , and kidneys were harvested 14 days later . ^^^ Apoptotic cells were identified by the Tunel technique , and the distribution of bcl 2 and bax was determined by immunochemistry . ^^^ In both adults and neonates , there was diffuse tubular bcl 2 and bax staining of sham operated and intact kidneys . ^^^ The distribution of bax staining in tubules of the obstructed kidney overlapped that of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of anti apoptotic members of the Bcl 2 family such as Bcl 2 and Bcl 10 ( L ) has been implicated in cancer chemoresistance , whereas high levels of pro apoptotic proteins such as Bax promote apoptosis and sensitize tumor cells to various anticancer therapies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Among biologic variables presently under investigation , apoptosis markers , in particular Bcl 2 and Bax expression , are receiving much attention for their relationship with the cellular response to genotoxic damage in experimental tumors . ^^^ Conversely , no or weak Bcl 2 expression , alone or in association with bax overexpression , appears indicative of a radiation response , and preliminary emerging evidence supports the involvement of such an association of apoptosis related markers even as predictors of long term response to neoadjuvant cytotoxic treatment . ^^^ Although the findings of an involvement of Bcl 2 and Bax as determinants of treatment response should be confirmed within the context of randomized clinical trials , they indicate a combined consideration of proteins that negatively and positively regulate apoptosis in translational studies on the effect of chemical and physical agents at a cellular level . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Four hours after AMPH injection , increase in c myc and decreases in Bcl 2 and Bcl 10 ( L ) mRNAs occurred in all tissues , whereas p 53 , Bax , and Bcl 10 ( S ) mRNAs increased in N 30 and HCC . ^^^ These changes disappeared in liver and N 12 at 18 hours , but persisted in N 30 and HCC . c Myc , P 53 , Bcl 2 , and Bax proteins in normal liver and HCC + / AMPH showed similar patterns . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further analysis indicated that the expression of the anti apoptotic protein bcl 2 was greatly reduced in the NSCLC cell lines H 460 and A 549 ( both p 16 , +p53 , +Rb ) , again only by the 5th day after Adv / p16 infection , but no affect on Bax expression was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , BH 4 oligopeptides of Bcl 2 and Bcl 10 ( L ) , but not mutant peptides , were able to inhibit both VDAC activity on liposomes even in the presence of Bax and apoptotic Deltapsi loss in isolated mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , 9 cis RA induced transiently the expression of p 21 ( Waf1 / Cip1 ) , p 27 ( Kip 1 ) , p 300 , CBP , BAX , Bak and bcl 2 proteins , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Bcl 2 homolog encoded by Kaposi sarcoma associated virus , human herpesvirus 8 , inhibits apoptosis but does not heterodimerize with Bax or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The authors examined apoptosis and the expression of apoptosis regulating proteins Bcl 2 , Bax , Bak , and Mcl 1 in 21 typical and 10 atypical bronchopulmonary carcinoid tumors . ^^^ METHODS : Thirty one bronchopulmonary carcinoid tumors were examined by using in situ 3 ' end labeling of DNA ( TUNEL ) for apoptosis and immunohistochemical staining methods for Bcl 2 , Bax , Bak , Mcl 1 , p 53 , and Ki 67 in formalin fixed , paraffin embedded tissue specimens . ^^^ No relation between apoptosis and Bcl 2 , Bax , Bak , or Mcl 1 expression was found . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , point mutations of 2 highly conserved residues within the BH 1 and BH 2 domains that abrogate the interaction of bcl 2 with bax , or deletion of the N terminal BH 4 domain , completely eliminate the ability of this molecule to up regulate NF kappaB dependent transactivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in p21WAF1 , pRb , Mdm 2 , Bax and Bcl 2 expression in cervical cancer cell lines transfected with a p 53 expressing adenovirus . ^^^ However , Ad 5 p53 transfection did not change the levels of Bax and Bcl 2 proteins . ^^^ Our results suggested that , Bax and Bcl 2 may not be important for the apoptosis of these cells , whereas cleavage of Rb , and the decrease of p21WAF1 could play important roles in p 53 dependent apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was also an up regulation of Bax , which altered the Bax : Bcl 2 ratio , which may be responsible for mitochondrial depolarization reported to be involved prior to the induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , in this study , we examined molecular markers known or suspected to be important in prostate cancer progression and key regulators of cell growth and apoptosis : p 53 , p21WAF1 / CIP1 , Bcl 2 , Bax , retinoblastoma ( Rb ) , and p16INK4A / MITS1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Lovastatin treatment resulted in decreased expression of the antiapoptotic protein bcl 2 and increased the expression of the proapoptotic protein bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 , inhibitor of apoptosis , was decreased with modification , while that of bax , inducer of apoptosis , increased only at early stage of the apoptotic pathway and decreased later . ^^^ CONCLUSION : In human breast cancer cells the induction of apoptosis by taxol was closely associated with mitotic arrest of cell cycle , and altered expression of bcl 2 and bax gene , possibly playing an important role in regulating taxol induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical evaluation for apoptosis related proteins , p 53 , Bcl 2 , Bax , caspase 1 and caspase 3 was also performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this report , we show that the opposing actions of MR and GR on neuronal survival result from their ability to differentially influence the expression of members of the bcl 2 gene family ; specifically , in the rat hippocampus , activation of GR induces cell death by increasing the ratio of the proapoptotic molecule Bax relative to the antiapoptotic molecules Bcl 2 or Bcl 10 ( L ) ; the opposite effect is observed after stimulation of MR . ^^^ In addition , we show that GR activation increases and MR activation decreases levels of the tumor suppressor protein p 53 ( a direct transcriptional regulator of bax and bcl 2 genes ) , thus providing new information on the early genetic events linking corticosteroid receptors with apoptosis in the nervous system . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An immunohistochemical study showed that the lymphoma cells were positive for leukocyte common antigen , Epstein Barr virus , bax . and bcl XL , and negative for L 26 and bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pro apoptotic activity of the Bcl 2 family member Bax has been shown to be facilitated by homodimerization . ^^^ Here we show by co immunoprecipitation and FPLC analyses that while Bax multimerizes and forms heterodimers with Bcl 2 , there is no evidence for Bcl 2 homodimerization , even in conditions under which Bcl 2 protects cells from apoptosis . ^^^ Immunofluorescence studies confirmed that Bax can attract active , soluble Bcl 2 to mitochondrial membranes , but that nuclear / ER membrane bound Bcl 2 was incapable of dislocating soluble Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bcl 10 , and bax expression in dysembryoplastic neuroepithelial tumors . ^^^ BACKGROUND : Bcl 2 , bcl 10 and bax are regulatory proteins which are variably expressed in brain tissue and are known to be involved in the regulation of apoptosis ; bcl 2 and bcl 10 inhibit apoptosis and bax generally promotes apoptosis . ^^^ The oligodendroglial like cells of DNT stained positively for bcl 2 in 2 / 17 tumors , bcl 10 in 10 / 17 tumors , and bax in 12 / 17 tumors . ^^^ The neuronal cell component of the DNT stained positively with bcl 2 in 15 / 17 tumors , bcl 10 in 5 / 17 tumors , and bax in 8 / 17 tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In rat mesangial cells treatment with thapsigargin decreased the expression of BCL 2 protein and bcl 2 mRNA , whereas it did not alter the levels of BAX protein or bax mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hair follicle ( HF ) growth and regression is an exquisitely regulated process of cell proliferation followed by massive cell death and is accompanied by cyclical expression of the apoptosis regulatory gene pair , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 inhibits a Fas induced conformational change in the Bax N terminus and Bax mitochondrial translocation . ^^^ Overexpression of the anti apoptotic protein Bcl 2 inhibited both the conformational change of Bax as well as its relocalization to the mitochondria . ^^^ These results suggest that the mechanism by which Bcl 2 inhibits Bax mitochondrial translocation and subsequent amplification of the apoptotic cascade is not by providing a physical barrier to Bax , but rather by inhibiting an upstream event necessary for Bax conformational change . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression patterns of the calcium binding proteins calbindin and parvalbumin and of the apoptosis modulating proteins Bcl 2 , Bax , and Bcl 10 were studied in the cerebellum of patients with multiple system atrophy ( MSA ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We demonstrated in the yeast two hybrid system that BFL 1 interacts strongly with human BAX but is not able to form homodimers nor to interact with human BCL 2 or BCL xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein levels of Bcl 2 and Bad in HL 60 and Molt 4 did not significantly change , and that of Bax was decreased after a 3 day incubation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BCL 2 family includes both proapoptotic ( e . g . , BAX and BAK ) and antiapoptotic ( e . g . , BCL 2 and BCL 10 ( L ) ) molecules . ^^^ We examined the function of BAX and BCL 10 ( L ) using genetic and biochemical approaches in budding yeast because studies with yeast suggest that BCL 2 family members act upon highly conserved mitochondrial components . ^^^ We also assessed the function of BCL 10 ( L ) and BAX in the same strain of Saccharomyces cerevisiae with deletions of selected mitochondrial proteins that have been implicated in the function of BCL 2 family members . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results showed that ( 1 ) simultaneous incubation with carbachol dose and time dependently blocked the specific DNA ladder formation induced by exposure to A ( beta 31 35 ) and ( 2 ) the A ( beta 31 35 ) induced downregulation of bcl 2 and upregulations of bax , p 53 , and c fos genes were reversed or ameliorated by the coadministration of carbachol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed baseline expression of the anti apoptotic Mcl 1 and pro apoptotic Bax proteins in immunoblots of eosinophil lysates , but not Bcl 10 , Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using kidneys , histologic examination and immunohistochemical detection of proliferating cell nuclear antigen ( PCNA ) , BrdU , Bcl 2 , and Bax were performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND MATERIALS : Protein levels of p 21 ( WAF1 / CIP1 ) , p 53 , bax , and bcl 2 were determined in 8 carcinomas ( 3 mammary carcinomas designated MCa 4 , MCa 29 , and MCa 35 , 2 squamous cell carcinomas designated SCC 4 and SCC 7 , ovarian adenocarcinoma OCa 1 , hepatocarcinoma HCa 1 , and adenosquamous carcinoma ACa SG ) syngeneic to C3Hf / Kam mice using Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to understand the functional relationship between the anti and pro apoptotic regulatory proteins in the cells under oxidant stress , we have studied the level of expression of apoptosis regulatory proteins , bcl 2 and bax , in human leukemia HL 60 cells . ^^^ Kinetic study demonstrated that the levels of both bcl 2 mRNA and protein expression were decreased with the progression of cellular apoptosis whereas the level of bax mRNA was unchanged but the expressed bax protein was not detectable . ^^^ These results suggest that the regulation of bcl 2 , but not of bax are important factor in the oxidative stress induced apoptosis in HL 60 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differences in bcl 2 and bax independent function in regulating apoptosis in sensory neuron populations . ^^^ Bcl 2 and Bax are cytoplasmic proteins that have antagonistic actions on apoptosis . ^^^ In contrast , the elimination of both Bcl 2 and Bax had different consequences for these populations of neurons . ^^^ This suggests that for nodose neurons , the suppression of apoptosis by Bcl 2 is entirely dependent on the presence of Bax . ^^^ This suggests that for trigeminal neurons , Bcl 2 functions partly independently of Bax in regulating survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast to previous studies , we found no evidence of Bad binding to anti apoptotic Bcl 2 , Bcl XL or McI 1 , or of alterations in Bax heterodimers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These were examined for the presence of apoptotic cells by the terminal deoxynucleotide transferase mediated nick end labeling method as well as by immunohistochemistry to detect possible expression of the apoptosis related genes p 53 , bcl 2 , and bax . ^^^ RESULTS : Bax expression was detected in all patients , whereas bcl 2 expression was noted in six patients . ^^^ This may have important clinical significance in the development of bcl 2 homologs or bax inhibitors to prevent apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bax and Bcl 10 were stronger in umbilical artery than in the neonatal aorta , but Bcl 2 was weak in both arteries in immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , mutations or protein expression of p 53 , bcl 2 and bax are reported in 13 non Hodgkin ' s B cell lymphomas of the head and neck . ^^^ These results suggest that inactivation of p 53 may be closely related to development and / or progression of these NHLs , and that bcl 2 and bax protein may not have a role . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These findings indicate that p 27 ( kip 1 ) may play important roles in mediating ceramide induced apoptosis and its expression can be regulated by Bax and Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A pro apoptotic Bcl 2 family protein , Bax , is largely present in the cytosol of many cells , but redistributes to mitochondria after treatment with apoptosis inducing drugs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic bodies were counted under a fluorescence microscope , and immunostaining was performed using anti Fas , Fas ligand , Bcl 2 , Bax , and p 53 antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Deltamethrin induces altered expression of P 53 , Bax and Bcl 2 in rat brain . ^^^ In this study we investigated the effects of deltamethrin on the expression of P 53 , Bax and Bcl 2 in rat brain . ^^^ These results indicate that deltamethrin leads to the persistent increase of P 53 and Bax expression and transient elevation of Bcl 2 expression , resulting in an increased ratio of Bax to Bcl 2 , which may contribute to apoptotic cell death in rat brain following deltamethrin treatment . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , BMP 2 , osteocalcin , and TGF beta , which are bone differentiating markers , apoptosis associating factors ( i . e . , Bcl 2 , Bax , Fas , and Fas ligand ) , apoptotic cells detected by the in situ 3 ' end labeling method ( TUNEL ) , and proliferating cell nuclear antigen ( PCNA ) were immunohistochemically examined in the heads ( in particular , the jaw bone and tooth germs ) of human fetuses of 11 week pregnancy . ^^^ Bcl 2 was clearly and Bax was weakly positive in the cells throughout the dental lamina and enamel organ . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax proteins are present in interphase nuclei of mammalian cells . ^^^ However , confocal fluorescence microscopy of human breast cancer cells and rat colon cancer cells immunostained with commercial antibodies raised against different epitopes of the anti apoptotic Bcl 2 and the pro apoptotic Bax protein revealed that these proteins are not only present in the cellular cytoplasm , but also within interphase nuclei . ^^^ In human cells , certain epitopes of Bcl 2 , but not of Bax , were also found to be associated with mitotic chromatin . ^^^ Transfection of human bcl 2 and bax into rat cells resulted in cytoplasmic and nuclear Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : DNA laddering by electrophoresis and the TUNEL method in histology for the final stage of apoptosis , Annexin 5 labeling , evidence of caspase activation , cleavage of substrates , measurements of mitochondrial pro apoptotic and anti apoptotic factors ( Bcl 2 , Bax and others ) and determination of the mitochondrial transitional pore potential . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have monitored changes in the growth factor signalling molecule MAPKinase , in the cytoprotective protein Bcl 2 and in the cell death protein Bax , during maturation of proliferating and non proliferating AML blasts in vitro . ^^^ By immunofluorescence / flow cytometry , there was no significant change in Bcl 2 over 7 d of culture , while Bax increased , particularly in cytokine free cultures ( 2 . 2 fold ) , which led to a significant decrease in the Bcl 2 / Bax ratio . ^^^ PD 98059 , however , reduced cell numbers in 7 d liquid culture and , in cytokine supplemented cultures , this was associated with a 1 . 3 fold increase in Bcl 2 ( p = 0 . 012 ) and a 1 . 4 fold increase in Bax ( p = 0 . 02 ) . ^^^ A rise in Bcl 2 may not protect AML blasts in the face of elevated Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis is regulated by the action of the Bcl 2 family of proteins , which includes anti and pro apoptotic members such as Bcl xS and Bax . ^^^ The Bax induced cell death was inhibited by co expression of Bax with Bcl 2 or Bcl xL , but was not inhibited by Z VAD FMK , NGF , or the Bcl 2 ml 3 or deltaC 22 mutants . ^^^ These results therefore suggest that Bax induces a caspase independent cell death pathway which is blocked by Bcl 2 but not by the NGF signaling pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 counterpart , Bax , which promotes PCD , displayed an increasing logarithmic profile with ascending DeltapO ( 2 ) regimen , such that the ratio of Bcl 2 / Bax decreased as pO ( 2 ) increased . ^^^ Pretreatment of fATII cells with l buthionine ( S , R ) sulfoximine , an irreversible inhibitor of gamma glutamylcysteine synthetase , the rate limiting enzyme in the biosynthesis of glutathione ( GSH ) , enhanced Bax and p 53 expression over Bcl 2 . ^^^ The GSH analogue , gamma glutamylcysteinyl ethyl ester , down regulated Bax / p53 abundance but restored that of Bcl 2 , thereby increasing Bcl 2 / Bax . ^^^ The antioxidant and GSH precursor N acetyl l cysteine favored Bcl 2 at the expense of Bax / p53 , whereas pyrrolidine dithiocarbamate induced Bax against Bcl 2 , with mild effect on p 53 . ^^^ Sulfasalazine , a potent and specific inhibitor of NF kappaB , induced Bax at the expense of Bcl 2 , in a p 53 dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of the molecular markers of apoptosis in isolated peripheral blood lymphocytes revealed an increase in the Bax : Bcl 2 ratio after treatment with Bryo 1 in cycles 2 and 3 , with associated poly ( ADP ribose ) polymerase cleavage after Bryo 1 and 2 CdA treatment . ^^^ In summary , we found that sequential treatment with Bryo 1 and 2 CdA caused a significant reduction in peripheral blood lymphocytes ( CLL cells ) with simultaneous induction of differentiation and the initiation of the Bax : Bcl 2 apoptotic pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BAX and PKCalpha modulate the prognostic impact of BCL 2 expression in acute myelogenous leukemia . ^^^ Because BCL 2 function can be modulated by dimerization with family members , like BAX , or by phosphorylation by protein kinase C alpha ( PKCalpha ) , we hypothesize that the relative expression of these proteins in primary leukemic cells might alter the prognostic impact of BCL 2 expression . ^^^ We therefore measured BAX and PKCalpha protein levels in peripheral blood mononuclear cell lysates from 165 newly diagnosed AML patients and correlated the expression of these proteins with BCL 2 expression , patient survival , and remission induction success . ^^^ However , the ratio of either BCL 2 : BAX ( B 2 : BX ) or PKCalpha * B2 : BX ( PK * B2 : BX ) was highly prognostic . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related proteins , bcl 2 , Bax , Fas and Fas ligand ( L ) , in ovarian epithelial neoplasms together with its clinical relevance was examined by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis was determined by identification of morphological features of apoptosis using time lapse videomicroscopy , identifying morphological changes in nucleis using Hoechst staining , quantitation of DNA fragmentation , level of expression of bcl 2 and bax proteins and identification of the proteolytic cleavage of Poly ( ADP ) ribose polymerase ( PARP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein expression of p 53 , p 21 ( WAF1 / CIP1 ) , bcl 2 , Bax , cyclin D 1 and pRb in human colon carcinomas . ^^^ Some of the more heavily studied proteins of importance for the control of growth arrest and apoptosis are p 53 , p 21 , bcl 2 and bax . ^^^ Overexpression of bcl 2 protein is thought to reduce the apoptotic capacity , while bax protein seems to be necessary for induction of apoptosis . ^^^ In this study , we have immunostained tissues from 93 primary colon carcinomas and have examined the expression of p 53 , p 21 ( WAF1 / CIP1 ) , bcl 2 bax , pRb and cyclin D 1 for evaluation of their roles in colon cancer progression . ^^^ Cyclin D 1 , bcl 2 and bax protein failed to have independent prognostic impacts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
They were , however , associated with the enhanced accumulation of the cell death inhibitor Bcl 2 , but not the cell death stimulator BAX , and with the heightened accumulation of the cell death regulator tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR results showed that IN down regulated Bcl 2 mRNA expression , and did not change Bax mRNA expression ; Western blot results confirmed that IN inhibited Bcl 2 protein expression , no influence was found on the translative level of bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied 5 FU and ZD 1694 induced TS inhibition in relation to the expression of p 53 , p 21 , Bcl 2 and Bax in six colon carcinoma cell lines , two with a wild type ( wt ) p 53 ( Lovo , LS174T ) and four with a mutant ( mt ) p 53 ( WiDr , WiDr / F , HT 29 and SW 948 ) phenotype . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the molecular pathway to apoptosis induction , members of the Bcl 2 family of proteins were examined ( Bcl 2 , Bcl 10 , Bax , and Bak ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The differences in immunohistochemical expression of p 53 , bcl 2 , bax , estrogen receptor ( ER ) , and progesterone receptor ( PR ) were evaluated in 40 endometrioid and 21 papillary serous carcinomas of endometrium and correlated with known predictors of survival , such as grade and stage . ^^^ No significant difference was found in bcl 2 and bax expression between both histologic types . ^^^ Bax and PR immunoexpression correlated near the limit of statistical significance ( p = . 08 ) , whereas no relationship was found among bax , bcl 2 , and ER immunopositivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein expression of bax , bcl 2 , and p 53 in patients with non Hodgkin ' s gastric lymphoma : prognostic significance . ^^^ The biologic significance of bcl 2 , bax , and p 53 gene expression in patients with non Hodgkin ' s gastric lymphoma is unknown . ^^^ Paraffin embedded specimens from 36 patients who underwent primary resection of the stomach for gastric lymphoma were analyzed immunohistochemically for p 53 , bax , and bcl 2 gene expression . ^^^ Expression of bax was seen in 24 of 36 patients ( 66 . 7 % ) , p 53 expression was found in 8 of 36 tumors ( 22 . 2 % ) , and bcl 2 cytoplasmic staining was detected in 6 of 36 patients ( 16 . 7 % ) . ^^^ In the bcl 2 negative ( ) patient group ( 30 patients ) , those who were bax positive had a statistically significant better survival than those who were bax negative ( 63 . 3 % vs . 36 . 7 % , p = 0 . 03 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas previous studies were performed on ex vivo cultures or on peripheral blood , our investigations included in vivo analysis of IM tonsillectomy specimens ( 14 cases ) by in situ hybridization for viral RNA ( EBERs ) combined with immunohistochemistry ( IHC ; CD 3 , CD45RO , CD 20 , CD79a , Ki 67 , Bcl 2 , Bax , Fas , FasL ) and the TUNEL method . ^^^ Through altered expression of Bcl 2 / Bax and Fas / FasL , the activated T cells are subject to enhanced apoptosis while residing within the lymphoid tissue , which eventually allows the efficient silencing of this potentially damaging T cell response . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MNU effectively induced hair follicular cell apoptosis at the anagen stage by up regulation of Bax protein without down modulation of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The signal to enter the apoptotic pathway is mediated by a shift in the ratio of Bcl 2 : Bax . ^^^ While bax levels remain constant , bcl 2 transcription rate , steady state mRNA , and protein levels decrease . ^^^ These studies suggest that NF kappaB mediates PCD in pro B cells through transcriptional repression of the survival gene bcl 2 , thus shifting the bcl 2 : bax ratio in favor of death promoting complexes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The data show that the engagement of CD 4 separately from TCR influences the expression of the proapoptotic protein Bax independently of the anti apoptotic protein Bcl 2 , whereas Ag activation coordinately modulates both Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We first analyzed mRNA expression of pro apoptotic Bak and Bax along with that of anti apoptotic Bcl 2 and Bcl xL , using breast cancer specimens of 27 patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein expression of Bcl 2 , Bax , Fas / Apo 1 in acute myeloid leukemia . ^^^ The protein expression of bcl 2 , bax , Fas / Apo 1 in 19 cases of acute myeloid leukemia ( AML ) were investigated by Western blot and ApAAp techniques . ^^^ High expression of bcl 2 protein was found in most of the AML cases , and some of the cases expressed Fas / Apo 1 and bax . ^^^ Bax seemed to coexpress with bcl 2 and so appeared to be a bad prognostic factor instead of a good one . ^^^ Coregulation of bcl 2 , Bax and Fas / Apo 1 genes formed the apoptosis induced pathway , which is the biological factor affecting response to chemotherapy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Study on the relationship between the Bcl 2 / Bax ratio and the growth types of leukemic cells and drug resistance in acute myelogenous leukemia . ^^^ The ratio of Bcl 2 to Bax ( Bcl 2 / Bax ) in 40 patients with acute myelogenous leukemia ( AML ) were determined . ^^^ The results showed that Bcl 2 / Bax in AML was significantly higher than that in normal control ( P < 0 . 001 ) . ^^^ Bcl 2 / Bax ratio in colony growth group was higher than that in no growth group ( P < 0 . 05 ) . ^^^ Between drug resistance group and drug sensitivity group there was a significant difference in Bcl 2 / Bax ratio ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Importantly , transfection of NF kappaB decoy ODNs significantly increased protein of bcl 2 , an inhibitor of apoptosis , and did not alter bax , a promoter of apoptosis , thereby resulting in a significant increase in the ratio of bcl 2 to bax ( P < 0 . 01 ) . bcl 2 mRNA was also decreased by hypoxia , whereas transfection of NF kappaB decoy ODNs significantly attenuated decrease in bcl 2 mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Endogenous levels of both Bcl 2 and Bax protein and p 53 status were evaluated . ^^^ However , the sensitivity of these cells to Bax varied and appeared to be independent of both the status of p 53 and the endogenous levels of bcl 2 or Bax , critical molecules in the apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , p21 / waf1 , bcl 2 , bax , Rb and Ki 67 proteins in Hodgkin ' s lymphomas . ^^^ The aim was to investigate the combined immunoexpression of p 53 , p 21 , bcl 2 , bax , Rb and Ki 67 proteins in Hodgkin ' s lymphomas ( HL ) and correlate expression patterns with the histotype and the Epstein Barr Virus ( EBV ) status . ^^^ P 53 , p 21 , bcl 2 , bax , Rb and Ki 67 proteins were detected in Hodgkin and Reed Sternberg ( HRS ) cells in 35 / 56 , 56 / 56 , 24 / 56 , 23 / 56 , 56 / 56 and 56 / 56 cases of HL , respectively . ^^^ Comparison between bcl 2 and bax staining showed a statistically significant relationship ( p < 0 . 001 ) for coexpression ( 19 cases ) or absence of expression of both proteins ( 28 cases ) in HRS cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , immunohistochemistry was performed for an array of apoptosis related proteins , i . e . the recently described apoptosis specific protein cJun / AP1 ( ASP ) , the proto oncogenes c Jun , c Jun AP 1 , Bcl 2 , Bax , Bcl 10 , p 53 , CD 95 ( Fas / Apo 1 ) , activated caspase 3 , several heat shock proteins ( alpha B crystallin , ubiquitin ) , and alpha synuclein . ^^^ There were no significant differences in the expression of c Jun , ASP , Bcl 2 , Bax , and Bcl 10 in substantia nigra neurons between PD , DLB , and controls nor between cortical and subcortical neurons with and without Lewy bodies . ^^^ No expression of p 53 , and activated caspase 3 , or any of the examined stress proteins was seen in neurons , while reactive astroglia and microglia were decorated by antibodies to Bcl 2 , Bax , alpha B crystallin and less , to Bcl 10 and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using reverse transcriptase polymerase chain reaction , cloning , and sequencing techniques , we have found that HL 60 cells express bak , bik , bax , bad , bcl 2 , bcl xL , bcl w , bfl 1 , fas , and caspases 1 4 and 7 10 . ^^^ Peripheral blood neutrophils expressed bak , bad , bcl w , bfl 1 , fas , and caspases 1 , 3 , 4 , and 7 10 , but hardly expressed bcl 2 , bcl xL , bik , bax , and caspase 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , although Bcl 2 could be readily coimmunoprecipitated with Bax , associations with Apaf 1 were undetectable under conditions where Apaf 1 interactions with procaspase 9 were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate the molecular mechanism of apoptosis of infiltrating T and other cells occurring in the CNS during autoimmune encephalomyelitis , we determined the type of apoptotic cells and the localization of apoptosis related molecules ( Fas , FasL , Bax , Bcl 2 and active caspase 3 ) by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bax , bcl 10 ( L ) and bcl 10 ( S ) expression in neoplastic and normal cervical tissue . ^^^ We simultaneously assessed bcl 2 , bax , bcl 10 ( L ) and bcl 10 ( S ) expression levels by Western blotting on 53 primary untreated cervical cancers and 15 normal samples . ^^^ Bcl 2 showed a trend to be lower in neoplastic than in normal samples ( P < 0 . 01 ) , while no significant difference was observed for bax and bcl 10 ( L ) . ^^^ Bcl 2 , bax and bcl 10 ( L ) levels in responding and non responding patients were not differently distributed . ^^^ Bcl 2 , bax and bcl 10 ( L ) are likely to play a role in the natural history of cervical tumors , but their clinical significance in predicting response to treatment and clinical outcome needs long term follow up studies . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The family can be divided into two classes : those such as Bcl 2 and Bcl xL that suppress cell death , and others , such as Bak and Bax , that appear to promote apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This is opened by pro apoptotic members of the Bcl 2 family such as BAX and prevented by anti apoptotic members such as Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblots showed no downregulation of Bcl 2 or Bcl xL and no upregulation of Bax , whereas decreased mitochondrial membrane potential was readily measurable 24 h after cytokine deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The earliest event induced by drug exposure was increase in Bad protein levels , followed by Bcl 2 down regulation , cytochrome c release , and Bcl xL and Bax up regulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Certain BCL 2 family proapoptotic proteins such as BAX and BAK share extensive sequence homology with BCL 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Other apoptosis inducing genes downstream of c myc , p 53 , p21WAF1 / CIP1 and Bax were significantly highly induced , although there was no induction of Bcl 2 , which prevents apoptosis in core expressing cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The impact of bcl 2 expression and bax deficiency on prostate homeostasis in vivo . ^^^ The histologic appearance of the prostates from probasin bcl 2 transgenic mice or bax / mice did not differ from those of control littermates . ^^^ There was no difference between probasin bcl 2 transgenic mice , bax / mice , and control littermates in steady state levels of apoptosis . ^^^ Following castration our findings suggest that both bax and bcl 2 may each contribute to the androgen responsiveness of prostatic glandular epithelial cells . ^^^ A comparison between the apoptotic indices in the ventral prostate from the probasin bcl 2 and bax / mice following castration suggests that the presence of bcl 2 may be a more important indicator of androgen sensitivity than a deficiency of bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CONCLUSIONS : These data demonstrate , at the molecular level , that bcl XL is selected as an apoptosis protective gene in place of bcl 2 while bax retains its dominant proapototic role . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of BCL 2 , BAX and BAK in the trophoblast layer of the term human placenta : a unique model of apoptosis within a syncytium . ^^^ We used immunohistochemistry with monoclonal antisera against human BCL 2 and polyclonal antisera against human BAX and BAK to study paraffin embedded sections of human term placentae ( n=5 ) from uncomplicated pregnancies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Histologic sections from the left and right ventricles of 16 hearts with impending myocardial infarction were stained with terminal deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling ( TUNEL ) method and with antibodies to p 53 , bcl 2 , cpp 32 , FAS , FAS L , and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The over expression of RA 538 and ASc myc could down regulate expression of c myc , bcl 2 and cyclinD 1 gene and up regulate expression of bax gene , but it could not regulate expression of p 53 , p 16 , TGase , and ras gene . ^^^ RA 538 and ASc myc relate to c myc , bcl 2 , cyclinD 1 and bax gene closely and have noticeable biologic effects on gastric cancer cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have studied by immunocytochemistry , Western blotting and RT PCR the expression pattern of Bcl xL , Bcl 2 and BAX in the in vitro model of neuronal differentiation constituted by retinoic acid ( RA ) treated NTera 2 / D1 ( NT2 / D1 ) cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Glucose treatment also mediated an upregulation of the cardioprotective factor Bcl 2 but did not affect the cellular content of the proapoptotic factors Bax and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of Bax and Bcl 2 in the brains of hamsters infected with 263K scrapie agent . ^^^ To study the mechanism ( s ) of neuronal cell death during scrapie infection , we investigated the expression of Bax and Bcl 2 in brains of hamsters infected with 263K scrapie agent . ^^^ The expression of Bcl 2 mRNA was significantly decreased in the brains of 263K scrapie infected hamsters compared with controls , whereas the expression levels of Bax mRNA were significantly increased in scrapie infected brain . ^^^ The levels of Bax and Bcl 2 proteins in brains of scrapie and control animals reflected the difference in mRNA levels . ^^^ Immunoreactivity for Bax and Bcl 2 were found predominantly within neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further studies showed that Hibiscus PCA application reduced Bcl 2 protein expression to 47 % , and increased Bax protein expression to 181 % after 1 . 5 hr as compared with time 0 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Indeed , multiple pro apopotic signal transduction pathways converge on the proteins of the Bcl 2 / Bax family which , in concert with the so called permeability transition pore complex ( PTPC ) , regulate mitochondrial membrane barrier function . ^^^ Prominent oncogenes ( e . g . , c Myc , Ras , Raf , Bcl 2 ) and tumor suppressor genes ( e . g . , p 53 , Bax ) have been shown to modulate apoptosis via a direct or indirect effect on mitochondrial membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The purpose of this study was to determine whether regression of the decidua basalis ( DB ) , which begins on Day 14 of pregnancy in the rat , results from an intrinsic program of apoptosis regulated by Bax and Bcl 2 . ^^^ Expression of Bax and Bcl 2 and the incidence of apoptosis were evaluated throughout gestation by Western blot analysis and detection of DNA fragments . ^^^ Bax , the pro apoptotic protein , was expressed at a low level throughout pregnancy , whereas Bcl 2 , the pro survival partner , was most abundantly expressed on Days 8 and 10 , which are a time of proliferation and decidualization , and declined to barely detectable levels thereafter . ^^^ These changes resulted in a 12 fold increase in the Bax : Bcl 2 ratio on Day 17 as compared with Day 8 of pregnancy ( P < 0 . 05 ) . ^^^ Treatment with RU 486 on Day 9 enhanced Bax and suppressed Bcl 2 within 6 h , increasing the Bax : Bcl 2 ratio sixfold ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we report that TSP 1 mediates endothelial cell apoptosis and inhibits angiogenesis in association with increased expression of Bax , decreased expression of Bcl 2 , and processing of caspase 3 into smaller proapoptotic forms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results were correlated with expression patterns of Bcl 2 and Bax , two proteins related to cell survival and cell apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunohistochemical techniques and Western blot analysis , the possible role of Bcl 2 family members Bax , Bcl 2 , Bcl 10 ( s ) , and Bcl 10 ( l ) in male germ cell density related apoptosis and DNA damage induced apoptosis was studied . ^^^ Furthermore , as Bcl 10 ( l ) , but not Bcl 2 , is present in spermatogonia and spermatocytes , Bcl 10 ( l ) may regulate germ cell density , possibly in cooperation with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To determine the role of bcl 2 , bcl 10 and bax in melanocytic tumors we investigated the differential expression of these genes via RT PCR in tissue samples from human benign nevi , primary melanomas and melanoma metastases in comparison with normal skin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When p 53 , c Myc , Bcl 2 , Bcl 10 ( L ) , and Bax were measured by flow cytometry and the activities of caspase 1 and caspase 3 like protease determined with Ac YVAD AMC or Ac DEVD AMC as substrates , the profile of ROS induced changes in these apoptosis regulatory and effector proteins suggests that elevation of c Myc , p 53 , and Bax and activation of caspase 3 play an important role in the apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Functional inactivation of both p 21 and bax ( by overexpression of Bcl 2 ) , but not either alone , allowed some bypass of p 53 induced growth arrest , indicating that multiple transcriptional targets of p 53 may mediate its antiproliferative action . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It promotes apoptosis by inducing the expression of the proapoptotic bax gene and inhibiting the antiapoptotic bcl 2 gene . ^^^ Matrix metalloproteinase 2 , p 53 , bcl 2 , and bax gene expression patterns were studied with quantitative competitive polymerase chain reaction . ^^^ This induction coincided with an increase in the expressions of the proapoptotic genes p 53 and bax and a drop in the expression of the antiapoptotic gene bcl 2 . ^^^ Deoxyribonucleic acid fragmentation , associated with elevations in the levels of the two proapoptotic gene products evaluated ( p 53 and bax ) and a drop in the level of the antiapoptotic bcl 2 , was seen in premature rupture of the fetal membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further investigate the significance of BAX expression , we performed immunohistochemistry on archival bone marrow biopsies and compared BAX staining to BCL 2 immunostaining . ^^^ An increase ( P < 0 . 05 ) in expression of both BAX and BCL 2 was detected in MM patients compared with patients with reactive plasmacytosis . ^^^ There was no correlation between BAX or BCL 2 expression and response to chemotherapy or duration of response or between BCL 2 expression and survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After determining that there were no CuZnSOD mutations present , we measured superoxide production from mitochondria and manganese superoxide dismutase ( MnSOD ) , glutathione peroxidase , NFkappaB , Bcl 2 and Bax by immunoblot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have examined , in a rat glioma cell line A15A5 , the effect of the stable transfection of human bcl 2 , bax and bcl xl on MMPs expression . ^^^ In bcl 2 and bcl xl transfected cells , the transcription of MMP 9 was decreased compared to that of control or bax transfected cells . ^^^ These results suggest that the ratio between MMP 9 and its inhibitor TIMP 1 is tightly controlled in cells overexpressing bcl 2 related proteins ( i . e . , high ratio in bax transfected A15A5 and low ratio in bcl 2 transfected A15A5 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis of Bcl 2 , Bcl xL and Bax expression and immunofluorescence analysis of promyelocytic leukemia ( PML ) protein indicated that apoptosis induced by spicamycin and KRN 5500 was associated with down regulation of Bcl 2 expression and modulation of PML protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the absence of Bax protein , HepG 2 cells with elevated Bcl 2 protein levels did not exhibit any significant increase in chemosensitivity towards the drugs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cyclin dependent kinase ( CDK ) was regulated by CDK inhibitor ( CDKI ) ( p 16 , p 15 ) , and CDK CDKI complex control RB by phosphorylation . p 53 regulate RB pathway through p 21 . p 53 is involved in apoptosis through bcl 2 or Bax 3 . p 19 coded by exon 1 beta and exons 2 and 3 of p 16 gene , binds to MDM 2 and regulate p 53 pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Oncoproteins from the Bcl 2 family and tumor suppressor proteins from the Bax family interact with PTPC to inhibit or facilitate membrane permeabilization , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial function , that is , incorporation of 3 , 3 ' dihexyloxacarbocyanine iodide [ DiOC ( 6 ) ( 3 ) ] and generation of reactive oxygen species ( ROS ) as well as expression of c Myc and Bcl 2 family members ( Bcl 2 , Bcl 10 ( L ) , Bax ) were evaluated by multiparameter flow cytometry . ^^^ Moreover , Bax protein was overexpressed in postG ( median fluorescence intensity = 180 , range 168 186 ) compared with preG cultures ( median fluorescence intensity = 75 , range 68 80 ; p < 0 . 01 ) , while no differences in Bcl 2 , Bcl 10 ( L ) , and c Myc staining intensity were observed . ^^^ Our findings demonstrate a humoral mediated rHuG CSF induced dissipation of lymphocyte mitochondrial Deltapsi ( m ) ; these effects might be mediated by Bax overexpression , with imbalance between apoptosis promoting and apoptosis inhibiting Bcl 2 family members and with subsequent induction of mitochondrial permeability transition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis by EB 1089 was associated with down regulation of the Bcl 2 protein without change of the Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To this end , leukemic blasts from pediatric patients with T ALL were prospectively investigated as to their responsiveness to IL 7 , IL 4 , and IL 2 ( in terms of modulation of spontaneous apoptosis , assessed by flow cytometry ) , cytokine receptor expression profiles , and expression levels of Bcl 2 and Bax proteins . ^^^ Multivariate analysis of the apoptosis related parameters investigated , including spontaneous apoptosis , its inhibition by IL 7 , and expression levels of Bcl 2 and Bax , showed that only IL 7 responsiveness has an independent impact on early cytoreduction ( P < . 05 ) , thus indicating a potential prognostic relevance of IL 7 sensitivity in T ALL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have examined the effect of cisplatin alone and in combination with theophylline , a phoshodiesterase inhibitor , on modulation of Bcl 2 / Bax expression and induction of apoptosis in human granulosa cells transformed by stable transfection with mutant p 53 plus Ha ras . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell proliferation , apoptosis , Bcl 2 and Bax expression in obstructed opossum early metanephroi . ^^^ MATERIALS AND METHODS : We have used CUTO in opossum pups with early metanephric kidneys , sacrificed at two weeks , to evaluate the role of cell proliferation , apoptosis and apoptosis regulating genes , Bcl 2 and Bax . ^^^ Apoptosis in the tubules is accompanied by increased Bax and decreased Bcl 2 staining . ^^^ Bcl 2 and Bax in the nephrogenic zone are unchanged compared with sham , but cell proliferation is increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family proteins Bax and Bcl 2 are the major regulators of apoptosis . ^^^ The aim of this study was to elucidate the involvement of the Bax / Bcl 2 system in induction of apoptosis of the inflamed colonic epithelium in UC . ^^^ Expression of CD 95 , Bax , Bcl xL , and Bcl 2 proteins was determined by western blotting . ^^^ The Bax / Bcl 2 system did not seem to be involved in induction of apoptosis of epithelial cells in the inflamed colonic mucosa of UC . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Morphologic and molecular genetic characteristics of keratinization and apoptosis in squamous cell lung carcinoma ] . 46 samples of squamous cell lung carcinoma ( SqCLC ) from surgical patients were examined for biomolecular tumor markers ( pancytokeratins , c myc , p 53 , bcl 2 , Bax , Ki 67 , IGF system ) and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the apoptotic marker CD 95 was analyzed by flow cytometry and the relative levels of CD 95 ligand , Bcl 2 and Bax protein were measured by immunoblotting . ^^^ In addition , it was found that the expression of CD 95 ligand and Bax increased and Bcl 2 decreased in the activated T cells from old rats compared to the activated T cells from young rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cryptorchidism induces mouse testicular germ cell apoptosis and changes in bcl 2 and bax protein expression . ^^^ We investigated the possibility that the testicular germ cell loss may be due to a change in the testicular temperature and apoptosis through the interplay of bcl 2 and bax proteins . ^^^ To investigate the effect of temperature on apoptosis and the role of bcl 2 and bax proteins in apoptosis in adult mouse testis , we used the experimental unilateral cryptorchidism model . ^^^ Biotin avidin DCS system indirect immunofluorescence technique indicated that the bcl 2 protein was chiefly distributed in the cytoplasm of germ cells in normal testicles , whereas the bax protein was chiefly expressed in the cytoplasm of germ cells in 6 to 15 days operated testicles . ^^^ Western blot analysis showed that the bcl 2 protein down regulated and the bax protein up regulated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Fas , Fas ligand , Bcl 2 , and Bax was assessed using immunohistochemistry . ^^^ There was no difference in the expression of Bax or Bcl 2 between groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Using Western blotting and immunohistochemistry , the authors investigated the expression of bcl 2 , bcl xL , bax , caspase 1 , and caspase 3 in temporal cortex samples from patients who had undergone temporal lobectomy surgery for intractable epilepsy ( n = 19 ) . ^^^ Bcl 2 , bax , and caspase 3 immunoreactivity was increased predominantly in cells with the morphologic appearance of neurons , whereas bcl xL immunoreactivity was increased in cells with the appearance of glia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The `` Bcl 2 rheostat ' ' may be pitched against apoptosis in colon cancer , inasmuch as overexpression of Bcl 2 , downregulation of Bak , and mutation of Bax are common defects in colon tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The degree of apoptosis was analyzed in relation to several clinicopathologic parameters , cell proliferative activity , and immunohistochemical expression of apoptosis related proteins , including bcl 2 , bax , bcl 10 , bak , p 53 , p 21 ( WAF1 / CIP1 ) , Fas , and Fas ligand . ^^^ Many synovial sarcomas were diffusely positive for bcl 2 family proteins ( bcl 2 , bax , bcl 10 , and bak ) and were negative or only sporadically positive for Fas , Fas ligand , p 53 , and p 21 ( WAF1 / CIP1 ) proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition to routine histologic review , we studied 12 representative cases with immunohistochemistry to examine expression of Ki 67 , epidermal growth factor receptor ( EGFR ) , Bcl 2 , and Bax . ^^^ Bcl 2 and Bax ( anti and pro apoptosis proteins , respectively ) and EGFR were expressed similarly in both normal and hyperplastic epidermis overlying the KMN . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , Bax and Fas in oxyphil cells of Hashimoto thyroiditis . ^^^ Immunoreactivity for bcl 2 , Bax and Fas was analysed in 16 cases with Hashimoto thyroiditis . ^^^ Bax was found to be weakly reactive or negative in normal thyrocytes and was not up regulated in bcl 2 deficient epithelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry showed that the majority of vital CD68+ macrophages surrounding caseous foci are negative for the anti apoptotic protein bcl 2 , but positive for the pro apoptotic protein bax . ^^^ These results suggest that caseation is strongly associated with apoptosis of macrophages and T lymphocytes ; that the onset of apoptosis in macrophages may be promoted by the lack of bcl 2 and the abundance of bax ; and that activation induced cell death ( AICD ) may be responsible for the apoptosis of T cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Influence of Bax or Bcl 2 overexpression on the ceramide dependent apoptotic pathway in glioma cells . ^^^ In the present study , the effects of Bcl 2 and Bax on the ceramide mediated apoptotic pathways were examined in glioma cells overexpressing Bcl 2 or Bax . ^^^ The growth of C 6 cells stably overexpressing either Bcl 2 or Bax was almost equal to that of the vector transfected cells . ^^^ These results indicate that Bax promotes apoptosis regardless of ceramide formation and that Bcl 2 or Bcl xL prevents ceramide formation by repressing neutral sphingomyelinase as well as ceramide induced cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The second part was to investigate the effect of CLA feeding on the development of histologically identifiable premalignant lesions in the rat mammary gland , as well as on the quantification of apoptosis ( by terminal uridyltransferase nick end labeling assay ) and the expression by immunohistochemistry of apoptosis regulatory proteins ( bcl 2 , bak , and bax ) in normal versus premalignant mammary structures . ^^^ It also significantly increased apoptosis and reduced the expression of bcl 2 in these lesions , but it did not modulate the levels of bak or bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To determine the activity of MAPKs and the level of Bax , Bcl 2 and p 53 proteins known to be involved in the regulation of apoptosis in pancreatic acini subjected to stressful stimuli leading to cell death . ^^^ In UV B irradiated cells , we did not detect any significant JNK activation as well as any significant changes in Bax , Bcl 2 and p 53 levels assessed by means of Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transgenic animal models and expression studies have shown that caspases , Bcl 2 , Bax , and possibly Bcl 10 are necessary players for the control of programmed cellular death in retinal ganglion cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECT : In an attempt to understand the roles of several apoptosis related genes in human glioma cells , the authors investigated the relationship of wild type p 53 , interleukin 1beta converting enzyme ( ICE ) , caspase 3 ( CPP 32 ) , bax , and bcl 2 to the apoptotic response of three glioma cell lines after treatment with etoposide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the apoptosis cascade , upregulation of Bax , downregulation of Bcl 2 , and increased CPP 32 protease ( caspase 3 ) activity were seen 12 hours after MNU dosing . ^^^ Therefore , the pathogenesis of MNU induced cataract was associated with DNA adduct formation in the lens epithelial cell nuclei leading to apoptosis by upregulation of Bax protein , downmodulation of Bcl 2 protein , and activation of caspase 3 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we applied wild type p 53 gene transfer to the rat osteosarcoma cells by lipofection to investigate the effects on cell growth , expression of genes such as waf1 / p21 , bcl 2 , and bax , and nucleosomal DNA fragmentation due to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sex hormone induced mammary carcinogenesis in the female Noble rats : expression of bcl 2 and bax in hormonal mammary carcinogenesis . ^^^ Based on these understandings , in the present study we examined the expression of Bcl 2 and Bax in pre malignant mammary glands from rats treated with different protocols of sex hormones for 7 weeks as well as sex hormone induced mammary tumours . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In investigating homeostatic cell death mechanisms in gustatory renewing epithelium , we observed that taste receptor cells were selectively immunopositive for the bcl 2 family death factor , Bax , and for the protease Caspase 2 ( Nedd2 / Ich1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We concentrated our observations on the apoptosis associated proteins bax and bcl 2 , on cytokeratin and vimentin as markers of differentiation , on desmosomes and on the antiadhesive epithelial membrane antigen episialin . ^^^ We found that the susceptibility of the surviving cells to apoptotic stimuli , measured by the bax / bcl 2 ratio , was much higher after a combined thermochemotherapy than after chemotherapy or thermotherapy alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , survival of the islet cells cultured under varying conditions was evaluated , and an assessment was performed of mRNA expression of Bcl 2 and Bax proteins . ^^^ The TUNEL technique and RT PCR showed , respectively , no major difference in cell survival and expression of mRNA encoding for Bcl 2 and Bax protein between control islets and islets kept for 24 h in the presence of high glucagon or high glucose . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in apoptosis related protein ( p 53 , Bax , Bcl 2 and Fos ) expression with DNA fragmentation in the central nervous system in rats after closed head injury . ^^^ At 4 h , Bax protein expression was elevated with a concomitant down regulation of Bcl 2 expression . ^^^ Double immunolabelling study has shown the colocalization of Bax immunoreactivity with Bcl 2 and p 53 . ^^^ Present findings suggest that the upregulation of p 53 and a shift in the ratio of Bcl 2 to Bax may contribute to neuronal apoptosis in the CNS after closed head injury . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related proteins , BCL 2 , BAX , FAS , FAS L and PCNA in liver biopsies of patients with chronic hepatitis B virus infection . ^^^ The expression of FAS , FAS L , BAX , BCL 2 , ICE and PCNA in the liver biopsies of 55 patients ( 14 HBsAg positive , 20 patients with alcoholic hepatopathy , 21 patients with other hepatopathies ) was tested by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , UA did not affect both the level of p 53 expression and the alteration of the balance between Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study the regulation of apoptosis in the endometrium during the normal menstrual cycle , the expression of the apoptosis related proteins Bcl 2 and Bax and their correlation to serum estradiol and progesterone , as well as to a replication related antigen Ki 67 were analyzed . ^^^ Apoptosis was studied quantitatively by Southern blot analysis of internucleosomal cleavage of genomic DNA , and qualitatively by using in situ 3 ' end labeling of fragmented DNA , and Bcl 2 , Bax and Ki 67 were detected and quantified immunohistochemically . ^^^ In human endometrium , apoptosis occurs predominantly in the late secretory and menstrual phases , and is related to alterations in the expression of Bcl 2 and Bax . ^^^ A decrease in the Bcl 2 / Bax ratio in the early secretory phase precedes DNA fragmentation and may predispose the cells to apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To explore the potential involvement of Bcl 2 family members in this process , the expression and localization of some Bcl 2 family proteins ( Bcl 2 , Bcl xL , Bcl w , Bak , Bax , and Bad ) and p 53 were analyzed during testicular development in the rat by Western blotting and immunohistochemistry . ^^^ The dynamic changes in the expression profiles of Bcl 2 family proteins are consistent with a model in which germ cells are primed for apoptosis during the first cycle of spermatogenesis by de novo expression of the death effectors Bax and Bad in a p 53 dependent manner and these proteins are prevented from triggering further apoptosis after the first spermatogenic cycle has been set up by anti apoptotic Bcl 2 family proteins Bcl xL and Bcl w . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time bcl 2 was down regulated as shown by Western blotting and flow cytometry , while no induction of bax was observed by ATRA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : The p 53 , bcl 2 , and bax genes are known to be involved in control of cell cycle progression and regulation of apoptotic cell death . ^^^ METHODS : Paraffin embedded tissue sections of 88 laryngeal squamous cell carcinomas that were diagnosed and treated between 1986 and 1996 were investigated for p 53 , bcl 2 , and bax protein expression by immunohistochemistry . ^^^ Other combinations of genes , i . e . , bcl 2 to bax and p 53 to bax ratios , were not associated with patient outcome . ^^^ However , protein levels of p 53 , bcl 2 , and bax were unrelated to proliferation and apoptosis of tumor cells . ^^^ The rate of apoptosis mainly counterbalanced proliferative activity but appeared not to be significantly influenced by p 53 , bcl 2 , and bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expressions of p 53 , Bcl 2 , Bax gene and proliferating cell nuclear antigen ( PCNA ) were also examined by immunohistochemical staining . ^^^ There was no significant correlation between AI and Bcl 2 or Bax staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax , and Bak protein expression in the hepatocytes was determined by immunohistochemistry method . ^^^ CONCLUSION : The overexpression of Bax , Bak proteins may play a role in HepG ( 2 ) cell apoptosis induced by ethanol and can be blocked effectively by Bcl 2 adenovirus vector . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prostate tumours from an Asian population : examination of bax , bcl 2 , p 53 and ras and identification of bax as a prognostic marker . ^^^ We examined 62 archival samples of prostate tumours from Asians of non Japanese origin for the over expression of p 53 , for the possible presence of mutated ras genes , for the overexpression of the bcl 2 and bax proteins , as well as directly for the presence of apoptotic cells by the TUNEL methodology . ^^^ While bcl 2 expression was not observed in any sample , bax expression was noted in 76 % of samples and was associated with a significantly worse patient prognosis both overall ( P < 0 . 005 ) and specifically in Chinese patients ( P < 0 . 02 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Barrett ' s esophagus , apoptosis and cell cycle regulation : correlation of p 53 with Bax , Bcl 2 and p 21 protein expression . ^^^ Bax is a member of the bcl 2 family whose function is that of cell death promoter . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of bcl 2 family gene transfectants revealed a down regulation of TNF induced apoptosis by bcl 2 and bclX overexpression , and an up regulation by bax gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of Bax / Bcl 2 proteins in pyogenic granuloma : a comparative study with granulation tissue and capillary hemangioma . ^^^ METHODS : PG ( 15 cases ) , GT ( 14 cases ) and capillary hemangioma ( CH , 8 cases ) were compared using in situ 3 ' tailing reaction ( ISTR ) , a histochemical method for identifying apoptotic cells , and immunohistochemistry for Ki 67 , Bcl 2 and Bax proteins , in order to clarify and involvement of apoptosis in the difference of biological characteristics between PG and GT . ^^^ RESULTS : PG showed significantly lower ISTR labeling indices than GT and CH and more frequent Bcl 2 / Bax expression than GT , whereas Ki 67 labeling indices were variable from case to case and did not show any significant differences among any groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We analyzed the relations between radiosensitivity and clinicopathological factors ( gender , tumor location , histological factors , and clinical tumor node metastasis stage ) , expression of apoptosis related proteins ( p 53 , bax , bcl 2 ) , apoptotic index using the terminal deoxynucleotidyltransferase mediated nick end labeling method , expression of cell proliferation related proteins ( Ki 67 labeling index and epidermal growth factor receptor overexpression ) and microvessel density ( MVD , vessels / field = 0 . 391 mm 2 ) in biopsy specimens from 31 LSCC patients given radiotherapy ( total radiotherapy dose of 52 70 Gy over 4 6 . 5 weeks ) . ^^^ No significant association between clinicopathological factors , or of overexpression of p 53 , bax , bcl 2 , epidermal growth factor receptor , or apoptotic index , with radiosensitivity was found . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fas , FasL , Bax , Bcl 2 , and pro and active caspase 3 were studied by immunohistochemistry . ^^^ The results indicate that type 2 cells can enter the Fas / FasL / caspase 3 pathway regulated by Bax and Bcl 2 . ^^^ High Bcl 2 : Bax levels favor type 2 cell survival and a low rate of apoptosis during hyperplasia . ^^^ Low Bcl 2 : Bax levels favor type 2 cell apoptosis during resolution . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bcl 10 ( L ) , and Bax was evaluated in both cell types by Western blot analysis . ^^^ C 6 glioma cells strongly expressed Bcl 10 ( L ) and only weakly expressed Bcl 2 and Bax , whereas SY5Y neuroblastoma cells expressed lower levels of Bcl 10 ( L ) and higher levels of both Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study examined the expression of some Bcl 2 family members , including Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and Bax , in response to cytokine stimulation in TF 1 and JYTF 1 cells in which SCF costimulation is differentially required for optimal proliferation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , mutation analysis was performed for Transforming growth beta type 2 receptor ( TGF beta R 2 ) , bcl 2 associated 10 protein ( BAX ) , Insulin like growth factor 2 receptor ( IGF 2 R ) , human MutS homolog 3 ( hMSH 3 ) , human MutS homolog 6 ( hMSH 6 ) , human MutS homolog 2 ( hMSH 2 ) , and human MutL homolog 1 ( hMLH 1 ) genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proliferating cells and apoptosis related gene products were assessed by immunohistochemistry for Ki 67 , p 53 , Bcl 2 , and Bax antigens . ^^^ Although the incidence of expression was significantly higher in carcinomas for p 53 and in adenomas for Bcl 2 than the others , the expression of apoptosis related gene products ( p 53 , Bcl 2 and Bax ) was similar among polypoid , flat elevated and depressed tumours . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An increase in the antiapoptotic Bcl 2 and Bcl xL protein levels and a decrease in the proapoptotic Bax and Bcl xS protein levels were also detected by Western blot analysis after SNAP treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax are differentially expressed in hyperplastic , premalignant , and malignant lesions of mammary carcinogenesis . ^^^ Here we evaluated for the first time the expression of cell death related proteins Bcl 2 and Bax in hyperplastic , premalignant ( carcinoma in situ ) , or malignant ( carcinoma ) lesions of mammary carcinogenesis ; we also assessed whether these proteins are involved in mediating Vz induced cell death in tumors . ^^^ We found that Bcl 2 and Bax were equally expressed in epithelial cells of terminal end buds , ducts , and alveoli . ^^^ However , in myoepithelial cells , the level of Bax expression was much higher than the level of Bcl 2 expression . ^^^ Bcl 2 and Bax levels in hyperplastic lesions were similar to those of normal mammary epithelial cells but lower in most carcinomas in situ and carcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 but not Bax or p 53 correlates with in vitro resistance to a series of anticancer drugs in breast carcinoma . ^^^ Among the factors controlling this process , a significant role is played by bcl 2 , bax and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that treatment of MCF 7 cells with Mlt for 24 h before the addition of atRA decreased the protein levels of the death suppressor , Bcl 2 , and increased , although with different time courses , the levels of the death promoters , Bax and Bak ; however , there was no change in the levels of the tumor suppressor gene , p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 10 ( L ) and Bid decreased significantly in the cadmium treated cells , although no apparent change in Bcl 2 and Bax expression was found . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Flow cytometric evaluation of p 53 , p 21 , Bcl 2 and Bax revealed no differences in in vitro expression levels between normal control and non lesional melanocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Deparaffinized tissue sections from 22 OLP and 10 control oral biopsy specimens were immunohistochemically stained with anti Bcl 2 , anti Bcl 10 , anti Bax and anti TNF alpha antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The oncoproteins Bcl 2 and Bax , the tumor suppressor gene product p 53 , TUNEL ( TdT [ terminal deoxynucleotidyl transferase ] dUTP nick end labeling ) and the cell cycle antigen Ki 67 were studied in 71 cases of mucoepidermoid carcinoma originating in the oral minor salivary glands . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Herein , we showed that inhibition of ERK1 / 2 activities caused ( 1 ) a G 1 arrest ; ( 2 ) a down regulation of the expression levels of the anti apoptotic homologs Bcl 2 , Mcl 1 , and Bcl 10 ( L ) without affecting the pro apoptotic levels of Bax and Bak ; ( 3 ) a promotion of caspases 3 , 6 , 8 , and 9 activities ; ( 4 ) a stimulation of PARP cleavage ; and ( 5 ) a programmed cell death by apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic members of the Bcl 2 protein family , including Bid and Bax , can activate apoptosis by directly interacting with mitochondria to cause cytochrome c translocation from the intermembrane space into the cytoplasm , thereby triggering Apaf 1 mediated caspase activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanisms of cell death and the role of Bcl 2 and Bax in a BCNU treated rat glioma cell line were investigated . ^^^ Our results indicate that apoptosis occurs only at a high concentration of BCNU with elevated levels of Bax and a reversed ratio of Bax / Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Importantly , the early release of cytochrome c from mitochondria closely correlates with the degradation of Bcl 2 and Bcl xL and a decrease in the ratios of Bcl 2 and Bcl xL to Bax during STA . ^^^ These findings suggest that the degradation of Bcl 2 and Bcl xL may favor Bax to induce cytochrome c release from mitochondria , which subsequently activates downstream caspases in STA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intravenous brain derived neurotrophic factor reduces infarct size and counterregulates Bax and Bcl 2 expression after temporary focal cerebral ischemia . ^^^ In this experiment we studied the effect of intravenous BDNF delivered after focal cerebral ischemia on neurological outcome , infarct size , and expression of proapoptotic and antiapoptotic proteins Bax and Bcl 2 , respectively . ^^^ The animals were then killed , and brains underwent either 2 , 3 , 5 triphenyltetrazolium chloride staining for assessment of infarct volume or paraffin embedding for morphology and immunohistochemistry ( Bax , Bcl 2 ) . ^^^ Bax positive neurons were significantly reduced in the ischemic penumbra in BDNF treated animals ( P : < 0 . 05 , unpaired , 2 tailed t test ) , whereas Bcl 2 positive neurons were significantly increased in this area ( P : < 0 . 001 , unpaired , 2 tailed t test ) . ^^^ As shown here , one possible mechanism of action of neuroprotection of BDNF after focal ischemia appears to be counterregulation of Bax / Bcl 2 proteins within the ischemic penumbra . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In studies of apoptosis associated proteins , BMP 2 was seen to down regulate the expression of Bcl 10 ( L ) ; however , BMP 2 had no effects on the expression of Bcl 2 , Bax , or Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CGP57148B treatment down regulated antiapoptotic XIAP , cIAP 1 , and Bcl 10 ( L ) , without affecting Bcl 2 , Bax , Apaf 1 , Fas ( CD 95 ) , Fas ligand , Abl , and Bcr Abl levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Stretching of Adp53m infected myocytes prevented stimulation of p 53 function that conditioned the expression of p 53 dependent genes ; quantity of angiotensinogen ( Aogen ) , AT ( 1 ) , and Bax decreased , whereas Bcl 2 increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The data also indicate that Bcl xL and Bax possess an ability to regulate mitochondrial membrane permeability independently of other Bcl 2 family members . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the current study , we investigated the mechanism by which tBid regulated cytochrome c release in terms of its relationship to mitochondrial permeability transition and Bax , another Bcl 2 family protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
One such gene is BAX , a proapoptotic member of the Bcl 2 family of proteins , which plays a key role in programmed cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of Bcl 2 , Bax and cytochrome c following focal cerebral ischemia and effect of hypothermia in rat . ^^^ Immunohistochemical analysis revealed Bcl 2 was induced in many neurons of HT group , while Bax and cytochrome c was induced in few neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of human osteoblasts with vitamin K 2 clearly suppressed Bax expression of the cells , although the expression of Bcl 2 was not influenced by vitamin K 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression of Bcl 2 , Bax and ICH 1 L in squamous cell cancer of the vulva . ^^^ STUDY DESIGN : Slides of 72 vulvar squamous cell carcinomas were stained immunohistologically for Bcl 2 , Bax and ICH 1 L . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mitochondrial permeability transition ( PT ) pore , also called the mitochondrial megachannel , is a multiprotein complex formed at the contact site between the mitochondrial inner and outer membranes , exactly the same location at which Bax , Bcl 2 and Bcl XL are particularly abundant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The latter molecule transactivates the expression of pro apoptotic genes , such as bax , and that of the cyclin dependent kinase inhibitor p 21 , whereas it down regulates the expression of the anti apoptotic protein bcl 2 . ^^^ Other protective effects of NO on apoptosis rely on the stimulation of cGMP dependent protein kinase ( PKG ) , modulation of the members of the bcl 2 / bax family that control the mitochondrial pore transition permeability , induction of the heat shock protein HSP 70 and interaction with the ceramide pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax expression and chlorambucil induced apoptosis in the T cells and leukaemic B cells of untreated B cell chronic lymphocytic leukaemia patients . ^^^ There was no differential expression of Bcl 2 or Bax between the CD 4 ( + ) and CD 8 ( + ) cells of B CLL patients , isolated by immunomagnetic separation . ^^^ We found correlations in the leukaemic B cells between chlorambucil ID ( 50 ) values and both Bcl 2 expression ( P=0 . 006 ) , and Bcl 2 / Bax ratios ( P=0 . 002 ) , suggesting a role for the Bcl 2 / Bax ratio in predicting the response of untreated CLL patients to cytotoxic treatment . ^^^ Chlorambucil produced almost identical changes in Bcl 2 and Bax expression in normal T cells and leukaemic B cells triggered to die by apoptosis , which together with the correlation between Bcl 2 and chemosensitivity confirms a pivotal role for Bcl 2 in regulating a distal step in the apoptotic pathway following cytotoxic cellular damage . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in cells isolated from B chronic lymphocytic leukemia patients at different stages of the disease . ^^^ In the present study we examined the expression of bcl 2 and bax , which has an antagonistic role against the function of bcl 2 , in cells from CLL patients at different stages of the disease , by immunoblot analysis . ^^^ In this study we found a trend of association between bcl 2 and bax levels . ^^^ Analysis including all patients revealed that 65 % of the patients who expressed high levels of bcl 2 had high levels of bax ( p = 0 . 058 ) . ^^^ Of patients in stage C , 45 % expressed high levels of both bcl 2 and bax while 50 % and 42 % of patients at stages A and B had low levels of both proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 3 modulates expression of Bax and Bcl 2 and potentiates p 53 independent radiation induced apoptosis in human breast cancer cells . ^^^ In MCF 7 , an increase in Bad and Bax protein expression and a decrease in Bcl 10 ( L ) protein and Bcl 2 protein and mRNA were observed . ^^^ In T47D , Bax and Bad proteins were up regulated ; Bcl 2 protein is undetectable in these cells . ^^^ Thus , IGFBP 3 increases the expression of pro apoptotic proteins and apoptosis both basally and in response to IR , suggesting it may be a p 53 independent effector of apoptosis in breast cancer cells via its modulation of the Bax : Bcl 2 protein ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Msh 2 , Mlh 1 , Fhit , p 53 , Bcl 2 , and Bax expression in invasive and in situ squamous cell carcinoma of the uterine cervix . ^^^ Immunoreactivity of Msh 2 , Mlh 1 , Fhit , p 53 , Bcl 2 , and Bax proteins was examined by immunohistochemical staining with appropriate antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and occurrence of Bcl 2 , Bak , Bax , Fas and FasL in the developing and adult rat endocrine pancreas . ^^^ At each time point , histologic sections were treated with the direct fluorescein labelled TUNEL method and immunostained for pancreatic hormones ( glucagon , insulin ) , apoptotic promoters ( Bak , Bax , Fas , Fas Ligand ) as well as for the anti apoptotic peptide Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : During induction of apoptosis , the pro apoptotic member of the Bcl 2 protein family ( Bax ) undergoes translocation to the mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For instance , analysis of bcl 2 , bax , p 53 , and caspase knockout mice while establishing distinct roles for each of these apoptotic players , they also provided valuable information for the design of specific inhibitors of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Western blot analysis showed that the heat shock induced Hsp 72 concomitant with an increase in Bcl 2 : Bax ratio . ^^^ An increase in the Bcl 2 : Bax ratio may in part explain the molecular mechanism of the effect of heat shock pretreatment . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , elucidation of the mechanism of apoptosis induction in thymocytes with respect to the involvement of apoptosis related genes revealed that the death pathway followed an interleukin 1 beta converting enzyme dependent , Fas mediated apoptotic cascade , with a concomitant increase in the protein products of the bax , bad , p 53 , fas and fasL genes and cleavage of the 23 kD N terminal fragment of Bcl 2 that exhibited Bax like death effector properties . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : By counting apoptotic biliary cells and by immunostaining apoptosis related proteins in normal liver , fatty liver , and those with acute viral hepatitis , chronic viral hepatitis , and hepatitis virus related cirrhosis , it was found that the larger the intrahepatic bile ducts became , the more biliary epithelial cells underwent apoptosis . bcl 2 , an inhibitor of apoptosis , was diffusely expressed in the interlobular bile ducts , but rarely detectable in the large and septal bile ducts . bcl XL and mcl 1 , inhibitors of apoptosis , and bax , a promoter of apoptosis , were diffusely expressed along the intrahepatic biliary tree . ^^^ CONCLUSION : The ratio of bax to bcl 2 , as well as the expression of CD 95 which differed at the interlobular versus large and septal bile ducts , may be responsible for the unique distribution of apoptotic biliary cells and involved in the homeostasis of the intrahepatic biliary tree . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the leukemic phases , the clonal cells were activated blasts expressing elevated levels of wild type ( wt ) p 53 , Bcl 2 , Bcl 10 ( L ) , and Bax , while Bak expression increased during the decline of lymphocytosis . ^^^ Bax heterodimerized with Bcl 2 but not with Bcl 10 ( L ) . ^^^ The anti apoptotic Bcl 2 / Bax heterodimers peaked during early leukemic phases and declined during regression . ^^^ The elevation in Bcl 2 , Bcl 10 ( L ) and Bax expression during early leukemic phases seems to result from cell activation since a similar increase was induced by activating the remission phase leukemic cells in culture . ^^^ The data suggest that wt p 53 , Bcl 10 ( L ) , and Bcl 2 / Bax heterodimers support the accumulation of activated leukemic cells during the leukemic phases , while Bax and Bak may be involved in their decline during regression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine the role of apoptosis in epithelial lung cells we investigated glyoxal dependent changes in pro and antiapoptotic mediators bax and activated caspase 3 , and galectin 3 and bcl 2 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 2 , its receptors , and bcl 2 and bax genes in normal , hyperplastic and carcinomatous human prostates : immunohistochemical comparative analysis . ^^^ The presence of interleukin 2 ( IL 2 ) and its receptors ( Ralpha , Rbeta , Rgamma ) , and their relationship with the products of bcl 2 and bax genes was studied in normal prostates , benign prostatic hyperplasia ( BPH ) , and prostatic cancer ( PC ) by ELISA , Western blot , and immunohistochemistry . ^^^ In BPH , immunoreactions were similar to that of normal prostates ( bax ) , similar to that of PC ( IL 2 and its three receptors ) , or intermediate between that of normal prostates and that of PC ( bcl 2 ) . ^^^ The increased expression of both bcl 2 and bax in PC might be involved in the higher apoptosis index reported in PC specimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of Bcl 2 and Bax in eutopic endometrium from women with endometriosis . ^^^ OBJECTIVE : To evaluate and compare spontaneous apoptosis and Bcl 2 and Bax expression in eutopic endometrium from women with and without endometriosis . ^^^ DESIGN : Apoptosis and Bcl 2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis . ^^^ MAIN OUTCOME MEASURE ( S ) : Apoptotic cells were detected with use of the dUTP nick end labeling ( TUNEL ) assay ; Bcl 2 and Bax expressions were assessed with use of immunohistochemical techniques . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We demonstrate that cell death induction is only partly dependent upon caspase activation and is not associated with modulation of bcl 2 , bax , or p 53 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the 8 / 61 A cells accumulated p 21 and Bax proteins while the level of the anti apoptotic protein Bcl 2 decreased implying a posttranscriptional regulation of apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of Bax and Bcl 2 in the assessment of cellular dynamics in fine needle samples of primary breast carcinomas . ^^^ Bax and Bcl 2 are members of a gene family that influence apoptosis and have been used as surrogate markers in the evaluation of this process . ^^^ Sixty three fine needle tumor samples from an equal number of patients with breast carcinomas were analyzed for Bax , Bcl 2 , and DNA content by flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In whole cells , the mutant protein efficiently heterodimerized with Bax , but exhibited impaired homodimerizationrelative to wild type Bcl 2 . ^^^ Together , the data indicate that the cysteine residues in Bcl 2 contribute , but are not absolutely essential , to the ability of Bcl 2 to homodimerize , heterodimerize with Bax , and suppress apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent experiments demonstrate that expression of several apoptotic regulatory proteins ( such as Bcl 2 , Bax , p 53 , and Survivin ) are differentially regulated according to the phases of the cell cycle . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
N terminal cleavage of bax by calpain generates a potent proapoptotic 18 kDa fragment that promotes bcl 2 independent cytochrome C release and apoptotic cell death . ^^^ Unlike the full length Bax , Bax / p18 did not interact with the antiapoptotic Bcl 2 protein in the mitochondrial fraction of drug treated cells . ^^^ In contrast , transfection of a cloned Bax / p18 cDNA into multiple human cancer cell lines targeted Bax / p18 to mitochondria , which was accompanied by release of cytochrome c and induction of caspase 3 mediated apoptosis that was not blocked by overexpression of Bcl 2 protein . ^^^ Therefore , Bax / p18 has a cytochrome c releasing activity that promotes cell death independent of Bcl 2 . ^^^ Finally , Bcl 2 overexpression inhibited etoposide induced calpain activation , Bax cleavage , cytochrome c release , and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of TP , estrogen receptor , Bcl 2 , Bax , p 53 , c erbB 2 and MIB 1 was examined by immunohistochemical methods . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further study by co transfection experiments indicated that Bid but not Bax ( both were pro apoptotic proteins of Bcl 2 family ) blocked the inhibitory effect of Bcl 2 on Fas mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was accompanied by upregulation of p 53 , Caspase 3 , and bax , whereas bcl 2 was downregulated as revealed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Myocyte apoptosis was measured by DNA laddering ; BCL 2 , BAX , Na ( + ) Ca ( 2+ ) exchanger , and SERCA 2 proteins by western blot ; and brain natriuretic peptide ( BNP ) , SERCA 2 mRNA by RT PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tempol did not affect the relative levels of Bax and Bcl 2 , whereas p 21 ( WAF1 / CIP1 ) was enhanced in a concentration and time dependent fashion ; this effect was partially inhibited by N acetylcysteine , was maintained for up to 8 h after Tempol removal , and seemed to depend on continuing protein synthesis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Translocation of Bax to mitochondria led to MMP , which was inhibited by microinjected Bcl 2 protein or bcl 2 transfection . ^^^ Our results establish that the following molecular sequence governs apoptosis of Env induced syncytia : Bax mediated / Bcl 2 inhibited MMP > AIF release > Cyt c release > caspase activation > nuclear apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In 56 specimens [ CCH , n=1 ; MTC with CCH , n=26 ; MTC , n=20 ; lymph node metastasis ( LNM ) , n=9 ] from 46 patients [ multiple endocrine neoplasia type 2a ( MEN2a ) , n=24 ; MEN2b , n=2 ; familiar MTC ( FMTC ) , n=4 ; sporadic MTC , n 16 ] and 3 cases of non neoplastic CCH , proliferation activity ( MIB 1 ) , the rate of apoptosis [ dUTP nick end labelling ( TUNEL ) ] and expression of p 53 , bcl 2 , bcl 10 and bax were investigated and compared with clinical data . ^^^ In MEN associated CCH and small MTC , bcl 2 was strongly expressed , bcl 10 was moderately expressed and bax was only weakly expressed . ^^^ Advanced tumours and LNM did show a more heterogeneous bcl 2 staining accompanied by an increased bax expression and accelerated proliferation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Fas and Bcl 2 did not change appreciably over the 8 d culture , but there was a gradual increase in Bax expression ; medium change , FCS and DMSO all partly inhibited Bax expression . ^^^ Proteolytic cleavage of poly ( ADP ribose ) polymerase and expression of Bax are likely to be mechanistically involved with the control of spontaneous apoptosis in ARL 6 cells , whereas changes in the levels of Fas and Bcl 2 do not play a role . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential regulation of Bcl 2 and Bax expression in cells infected with virulent and nonvirulent strains of sindbis virus . ^^^ In this study we examined the effects of Sindbis virus on cell apoptosis and on the expression of Bcl 2 and Bax . ^^^ SVA , which infects cells in a persistent manner , induced up regulation of bcl 2 mRNA and Bcl 2 protein , whereas SVNI induced an increase in Bax levels . ^^^ Our results indicate a differential regulation of Bcl 2 and Bax expression by SVA and SVNI , which may be associated with the apoptotic potential of the viruses . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Heterodimerization of Bcl 2 and Bcl 10 ( L ) with Bax and Bad in colorectal cancer . ^^^ The rate of cell loss owing to apoptosis is mediated by competitive dimerization with selective pairs of cell death antagonists ( Bcl 2 , Bcl 10 ( L ) ) and agonists ( Bax , Bad ) . ^^^ We analyzed the expression of Bcl 2 , Bcl 10 ( L ) , Bax , and Bad in normal appearing mucosa and colorectal tumor tissues by Western blotting after immunoprecipitation . ^^^ Compared with the ratio of Bax Bcl 2 / total Bax in normal mucosa , the ratio was significantly reduced in tumors ( p = 0 . 02 ) . ^^^ A reduced heterodimerization of Bax with Bcl 2 may contribute to the development of colorectal cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of p 53 , Bax , and Bcl 2 , determined by immunoblotting , revealed a dose dependent increase in p 53 and Bax , but the level of Bcl 2 remained unchanged . ^^^ These data suggest that the high constitutional level of Bcl 2 may protect melanocytes from UVB induced injury , and that apoptotic death of melanocytes may be induced by the elevation and redistribution of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bax , and caspase 3 in the brain of the developing rat . ^^^ A quantitative immunoblotting technique was used to examine the temporal expression of bcl 2 , bax , and two isoforms of caspase 3 ( an active 20 kDa isoform and the inactive 32 kDa precursor ) throughout the developing neuraxis . ^^^ In general , the expression of bax increased and the ratio of bcl 2 expression to bax expression decreased concurrent with published data on the onset of NOND in a given area . ^^^ Reductions in caspase expression ( and not bcl 2 and bax expression ) are key to ending the period of NOND . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ordering of ceramide formation , caspase activation , and Bax / Bcl 2 expression during etoposide induced apoptosis in C 6 glioma cells . ^^^ In addition , exposure of cells to etoposide resulted in decreased expression of Bcl 2 with reciprocal increase in Bax protein . z VAD . ^^^ FMK , a broad spectrum caspase inhibitor , failed to suppress the etoposide induced ceramide formation and change of the Bax / Bcl 2 ratio , although it did inhibit etoposide induced death of C 6 cells . ^^^ Reduced glutathione or N acetylcysteine , which could reduce ceramide formation by inhibiting sphingomyelinase activity , prevented C 6 cells from etoposide induced apoptosis through blockage of caspase 3 activation and change of the Bax / Bcl 2 ratio . ^^^ In contrast , the increase in ceramide level by an inhibitor of ceramide glucosyltransferase 1 , D threo 1 phenyl 2 decanoylamino 3 morpholino 1 propanol caused elevation of the Bax / Bcl 2 ratio and potentiation of caspase 3 activation , thereby resulting in enhancement of etoposide induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax mammalian regulators of apoptosis are functional in Drosophila . ^^^ CED 9 belongs to the Bcl 2 family which includes negative ( Bcl 2 ) and positive ( Bax ) regulators of apoptosis . ^^^ Expressing bcl 2 or the pro apoptotic mammalian bax in the developing eye and wing alters these structures , bcl 2 increasing the number of cells , while bax reduces the number of cells . ^^^ In addition , the functional interaction between Bcl 2 and Bax is conserved . ^^^ These results indicate that factors necessary for the activity of bcl 2 and bax are present in Drosophila . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tolerance Inducing dose of 3 nitropropionic acid modulates bcl 2 and bax balance in the rat brain : a potential mechanism of chemical preconditioning . ^^^ The current study investigated the mRNA and protein expression of the antiapoptotic bcl 2 and the proapoptotic bax . two antagonistic members of the bcl 2 gene family , in response to a single dose of 3 NPA , to global cerebral ischemia reperfusion . and to the combination of both 3 NPA pretreatment and subsequent global cerebral ischemia reperfusion . ^^^ Brain homogenates of adult Wistar rats ( n = 25 ) were analyzed for bcl 2 and bax mRNA expression using a new highly sensitive and quantitative polymerase chain reaction ( PCR ) technique that allows real time fluorescence measurements of the PCR product ( LightCycler ; Roche Diagnostics , Mannheim , Germany ) . ^^^ Immunohistochemistry was applied to serial brain sections of additional rats ( n = 68 ) to determine amount and localization of the respective Bcl 2 and Bax protein expression in various brain areas . ^^^ The authors found single dose 3 NPA treatment to be associated with an elevated bcl 2 : bax ratio ( increased bcl 2 expression , decreased bax expression ) , both on the transcriptional ( mRNA ) and the translational ( protein ) level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / Bax protein ratio predicts 5 fluorouracil sensitivity independently of p 53 status . p 53 tumour suppressor gene is involved in cell growth control , arrest and apoptosis . ^^^ Moreover , after 5 FU exposure , Bax and Bcl 2 proteins regulation was under p 53 protein control and a statistically significant relationship ( r = 0 . 880 , P = 0 . 0097 ) was observed between Bcl 2 / Bax ratio and 5 FU sensitivity . ^^^ In conclusion , whatever p 53 status , Bcl 2 or Bax induction and Bcl 2 / Bax protein ratio were correlated to 5 FU sensitivity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of allyl sulfur compounds and garlic extract on the expression of Bcl 2 , Bax , and p 53 in non small cell lung cancer cell lines . ^^^ DAS and garlic extract treatment of H 460 cells induced a rise in the level of Bax and a fall of Bcl 2 level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax , Bcl 2 , and P 53 in progressive multifocal leukoencephalopathy . ^^^ Cellular factors , Bax and Bcl 2 , are two essential downstream elements involved in p 53 dependent apoptosis . ^^^ To determine whether association of JC virus with p 53 protein affects the expression of Bax and Bcl 2 in viral infected cells in progressive multifocal leukoencephalopathy ( PML ) , we studied the expression of Bax , Bcl 2 , and p 53 in 14 cases from 13 PML patients by using paraffin immunohistochemistry . ^^^ The expression pattern of Bax positive / Bcl 2 negative in viral infected glial cells suggests that the oligodendrocyte may be undergoing apoptosis which may in turn contribute to the demyelinating process in PML . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 blocks cytochrome c release and apoptosis but has no effect on Bax translocation , suggesting that Bax translocation acts upstream of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
UVC induced apoptotic symptoms such as morphological changes , DNA fragmentation , Bcl 2 and Bax protein expression were examined in primary splenocyte cultures from young ( 3 months ) and old ( 24 months ) rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no correlation between expression of members of the Bcl 2 family ( Bcl 2 , Bcl xL , Bax , and Bak ) and TRAIL sensitivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the multidrug resistance glycoprotein 1 ( MDR 1 ) , bcl 2 and bax was quantitatively assessed by immunoblotting . ^^^ Correlation analysis showed that : ( 1 ) resistance was associated with longer DTs , but this was generally abated by a 96 h exposure ; ( 2 ) with a 3 h exposure , the combination of MDR , bcl 2 and bax parameters with DT ( DT + MDR + bcl 2 bax ) best correlated with IC 50 values ( r = 0 . 77 ) ; ( 3 ) with a 96 h exposure , in spite of the generally decreased IC 50 values , a combination of MDR 1 , bcl 2 and bax parameters ( MDR + bcl 2 bax ) best correlated with the IC 50 values ( r = 0 . 71 ) . ^^^ CONCLUSIONS : These results suggest that the exposure duration , DT , and expression of MDR 1 , bcl 2 and bax each contribute to paclitaxel sensitivity of human colorectal carcinoma cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After PDT , expression of Bcl 2 , Bcl 10 ( L ) , Bax , and Bak was also examined in cell lysates by Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , DR ( + ) leukaemia like cells also overexpress Bcl 2 , while bax expression is suppressed , compared with the wild type ( wt ) parental haematopoietic stem cell line . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , it was suggested that butein induces apoptosis via down regulation of Bcl 2 expression and promotion of Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The release of cytochrome c can be influenced by different Bcl 2 family member proteins , including , but not limited to , Bax , Bid , Bcl 2 , and Bcl 10 ( L ) . ^^^ Bax and Bid potentiate cytochrome c release , whereas Bcl 2 and Bcl 10 ( L ) antagonize this event . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study was designed to 1 ) investigate whether apoptosis is responsible for the atresia of nonovulatory dominant follicle ( DF ) , 2 ) to determine if atresia of a nonovulatory DF is associated with alterations in Bcl 2 and Bax expression , 3 ) to test whether progesterone P ( 4 ) has a direct effect on apoptosis in bovine follicles , and 4 ) to study the pattern of expression of Bcl 2 and Bax in follicles at different developmental stages ( small , medium , and large ) . ^^^ Expression of Bcl 2 and Bax proteins was examined by Western blot analysis . ^^^ Furthermore , both Bcl 2 and Bax were found to be present in all DFs ; however , the ratio of Bcl 2 and Bax protein levels was significantly reduced by 24 h of P ( 4 ) treatment compared with DFs from the control group ( P < 0 . 05 ) . ^^^ In addition , the pattern of expressions of Bcl 2 and Bax in follicles at different developmental stages ( small , medium , and large ) was studied . ^^^ The ratio of Bcl 2 and Bax protein levels was variable in small follicles ; however , Bax protein level was always relatively higher than that of Bcl 2 in medium and large follicles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This is accompanied by progestin up regulation of the antiapoptotic protein bcl xL , no effect on the proapoptotic protein bax , and , surprisingly , diminution of the antiapoptotic protein bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with this , overexpressing active Akt rescues cells from apoptosis without altering expression levels of endogenous Bcl 2 , Bcl 10 , or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of Bax and Bcl 2 in snuff and non snuff associated oral squamous cell carcinomas . ^^^ MATERIALS AND METHODS : OSCC from Sudanese toombak users ( n = 13 ) and non users from the Sudan ( n = 6 ) and Norway ( n = 24 ) were analysed for bax , bcl 2 and Ki 67 immunohistochemically . ^^^ No significant differences were detected in apoptosis , bax , bcl 2 and Ki 67 in OSCC from the Sudan in relation to toombak use or p 53 gene status . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the expression of several genes involved in differentiation , apoptosis and chemoresistance : ets 1 , bax , bcl 2 , bag 1 , bcl 10 , mdr 1 and mrp . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of TBI on regional cellular patterns of expression of survival promoting proteins such as Bcl 2 , Bcl xL , and extracellular signal regulated kinases , and death inducing proteins such as Bax , c Jun N terminal kinase , tumor suppressor gene , p 53 , and the caspase family of proteases are reviewed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of these , hsp 70 , CAS , bcl 2 and bax were studied . ^^^ In addition , the expression of the bcl 2 / bax protein decreased significantly in these two cell lines treated with TCHQ but not PCP . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Messenger RNA expression levels of c myc , p 21 , bax , bcl 2 , and bcl 10 were not changed by the treatment with troglitazone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , expression of antiapoptotic bcl 2 or proapoptotic Bax did not change significantly under the experimental conditions used , indicating that the regulation of apoptosis is complex and may be influenced by different factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ado induced cell death was characteristic of apoptosis , and Ado increased the expression of a pro apoptotic product Bax but decreased that of an anti apoptotic product Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Under this condition , bcl 2 mRNA levels were significantly increased over time by retinoid treatment , whereas bax mRNA levels were not affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Various factors which may modulate the apoptotic response to DNA damage include the p 53 status of the cell , levels and activity of the Bax and Bcl 2 families of proteins , activation of NF kappa B , relative levels of insulin like growth factor and insulin like growth factor binding proteins , activation of MAP kinases and PI3 / Akt kinases , ( the absence of ) ceramide generation and the CD 95 ( APO1 / Fas ) signaling pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no significant differences in the level of expression of Fas , Bcl 2 , and Bax between the three groups but addition of transforming growth factor ( TGF ) beta 1 significantly inhibited Fas mediated apoptosis in hypertrophic scar derived and normal skin derived fibroblasts and neutralization of autocrine TGF beta 1 with anti TGF beta 1 antibody abrogated the resistance of keloid derived fibroblasts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some authors have proposed that apoptosis is dependent on induction of the mitochondrial permeability transition pore ( PTP ) , and that activators of apoptosis such as Bax work through activation of PTP , whereas inhibitors of apoptosis such as Bcl 2 work through inhibition of PTP , and the consequent activation or inhibition of PTP dependent release of mitochondrial apoptotic factors , including cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Physiologic concentrations of genistein ( < or = 20 microM ) decreased LNCaP viable cell number in a dose dependent manner , induced a G ( 1 ) cell cycle block , decreased prostate specific antigen mRNA expression , and increased p 27 ( KIP 1 ) and p 21 ( WAF 1 ) ( mRNA and protein ) but had no effect on apoptosis or the mRNA expression of the apoptosis and cell cycle related markers bcl 2 , bax , Rb , and proliferating cell nuclear antigen . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical stains for cytokeratins , involucrin , and apoptosis related proteins such as Bcl 2 , Bcl 10 ( L ) , and Bax were done . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
None of the factors studied was of independent prognostic significance by itself , but when Bax and Bcl 2 expression data were considered together , this combined variable was of independent prognostic significance ( P = . 0115 ) , together with residual disease status ( P = . 0016 ) , differentiation grade ( P = . 0014 ) , and the presence of ascites ( P = . 0122 ) . ^^^ Patients with a long median survival ( 104 months ) could be discriminated from those with a short one ( 16 months ) by combining the individual patients ' expression data for p 53 , Bax , and Bcl 2 with their residual disease status ( P < . 00001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential sensitivity of MCF 7 and LCC 2 cells , to multiple growth inhibitory agents : possible relation to high bcl 2 / bax ratio . ^^^ It was found that bcl 2 protein and mRNA were elevated and that bax protein and mRNA were reduced in LCC 2 compared with MCF 7 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Endonuclease activity and expression of the antiapoptotic protein Bcl 2 and the proapoptotic proteins Bax and caspase 3 were also evaluated in LV tissue . ^^^ Western blotting for Bcl 2 , Bax and caspase 3 showed increased expression of Bcl 2 , decreased expression of caspase 3 and no change in Bax in MET treated compared with untreated dogs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that dexamethasone activated endogenous and exogenous GR inhibit p 53 dependent functions , including transactivation , up ( Bax and p 21 ( WAF1 / CIP1 ) ) and down ( Bcl 2 ) regulation of endogenous genes , cell cycle arrest and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , mitochondria are the major sites of action of Bax and other apoptotic regulatory proteins of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify possible pathways of apoptosis , we evaluated the expression of phosphorylated p 38 mitogen activated protein kinases , bax , bcl 2 , cytochrome c , and peroxisome proliferator activated receptor alpha ( PPAR alpha ) with Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of Mo7e leukemia cells is associated with the cleavage of Bcl 2 into a shortened fragment that is not functional for heterodimerization with Bcl 2 and Bax . ^^^ Both Bcl 2 / Delta34 and Bax proteins generated in wheat germ extract were readily relocated to the mitochondria isolated from control Mo7e cells . ^^^ Insertion of Bax , but not Bcl 2 / Delta34 , into mitochondria triggered a rapid release of cytochrome c from the mitochondria . ^^^ Coimmunoprecipitation studies showed that , unlike Bcl 2 , the cleaved Bcl 2 fragment was no longer functional for dimerization with either Bcl 2 or Bax . ^^^ Taken together , these findings showed that the integrity of Bcl 2 is necessary for its function of heterodimerization with Bax , which appears to be one of the mechanisms of antiapoptotic effect of Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Conversely , BAX and BCL 2 , two other genes associated with regulation of apoptosis , showed no overall correlation with drug sensitivities . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis revealed that the content of Bcl 10 ( L ) ( but not of Bcl 2 , BAX , Bad , and Bim ) significantly decreased in thymocytes after CLP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To delineate further the pathways involved , we examined the temporal changes in proapoptotic Bax and antiapoptotic Bcl 2 in rat testes after transient exposure to heat ( 43 degrees C for 15 min ) . ^^^ Despite the striking redistribution , Bax levels remained unchanged as determined by Western analysis ; Bcl 2 levels increased significantly by 6 h after heat exposure . ^^^ Reverse transcription polymerase chain reaction analysis indicated no change in either Bax or Bcl 2 mRNA levels in response to heat , suggesting the involvement of post transcriptional rather than transcriptional mechanisms mediating their activity . ^^^ The marked subcellular redistribution of Bax prior to activation of apoptosis and the increase in Bcl 2 suggest an involvement of Bcl 2 family members in heat induced apoptotic death of germ cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of spontaneous apoptosis , bcl 2 and bax were evaluated in 56 newly diagnosed AML patients to determine if they correlated with a response to cytotoxic therapy . ^^^ Spontaneous apoptosis was lower , but bcl 2 , bax and the bcl 2 / bax ratio were higher in AML compared with normal individuals . ^^^ There was a significant reduction in bcl 2 and bax expression when AML patients entered complete remission and also in relapsed AML patients who entered a second remission . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The low incidence of T cell apoptosis in AE was associated with significantly increased levels of Bcl 2 relative to Bax ( P < 0 . 0001 ) and significantly decreased CD 95 L expression ( P < 0 . 002 ) compared with the resolving MR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We conducted a retrospective immunohistochemical evaluation of the prognostic significance of the expression of p 53 and the related proteins Bax , Bcl 2 , growth arrest and DNA damage ( Gadd 45 ) , murine double minute 2 ( Mdm 2 ) and p 21 ( WAF1 / CIP1 ) in chemonaive tumours taken from 66 patients with ovarian cancer . ^^^ The expression of each of the following proteins was significantly associated in the tumours ( P < 0 . 05 unless otherwise stated ) : Bax with Bcl 2 ( P < 0 . 01 ) ; Bax with Mdm 2 ; p 21 ( WAF1 / CIP1 ) with Gadd 45 ( P < 0 . 01 ) ; p 21 ( WAF1 / CIP1 ) with p 53 ; p 53 with Mdm 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis did not display variations in the expression of p 53 , Bcl 2 , Bcl XL , and Bax proteins during the treatment with AMD and DEA . ^^^ These data indicate that AMD induces cytochrome c release from mitochondria , triggering apoptosis through an iodine independent mechanism , and that this process is not mediated by modulation of p 53 , Bcl 2 , Bcl XL , or Bax protein expression and does not involve the generation of free radicals . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in the human corpus luteum during the menstrual cycle and in early pregnancy : regulation by human chorionic gonadotropin . ^^^ To investigate the relationship between apoptosis and the Bcl 2 / Bax system in the human corpus luteum ( CL ) , the frequency of apoptosis and expression of Bcl 2 and Bax were examined in the CL during the menstrual cycle and in early pregnancy . ^^^ In contrast , Bax immunostaining was observed in the regressing CL , but not in the midluteal phase and early pregnancy . bcl 2 messenger ribonucleic acid ( mRNA ) levels in the CL during the menstrual cycle were highest in the midluteal phase and lowest in the regressing CL . ^^^ Western blot analyses revealed that Bcl 2 expression was significantly lower in the regressing CL than in the midluteal phase and early pregnancy , and that Bax expression was , in contrast , significantly higher in the regressing CL than in the midluteal phase and was remarkably low in the CL of early pregnancy . ^^^ When corpora lutea of the midluteal phase were incubated with hCG , hCG significantly increased the mRNA and protein levels of Bcl 2 and significantly decreased those of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The resistance of U 266 cells to anti Fas did not appear to reflect dysregulation of Bcl 2 , Bcl 10 ( L ) , and Bax , because these proteins were expressed in both RPM 18226 and U 266 cells to similar levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax immunohistochemistry in denervation reinnervation and necrosis regeneration of rat skeletal muscles . ^^^ Bcl 2 and Bax immunohistochemistry was examined in the skeletal muscle of rats after cutting the sciatic nerve , as a model of denervation and reinnervation , and in the anterior tibialis muscle of rats after an intramuscular injection of metoclopramide , as a model of necrosis and regeneration of muscle fibers , to better understand the role of these proteins in muscle disorders . ^^^ Bcl 2 and Bax immunoreactivity was limited to macrophages in necrotic muscle fibers at 24 h after the intramuscular injection of metoclopramide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced by droloxifene and c myc , bax and bcl 2 mRNA expression in cultured luteal cells of rats . ^^^ In the present study , the expression of c myc , bax and bcl 2 mRNA in cultured rat luteal cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed . ^^^ The results showed that the c myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased , while the bcl 2 mRNA level exhibited no changes . ^^^ The increase of c myc mRNA expression might be one of the initiating factors and the elevated ratio of bax / bcl 2 mRNA was also probably involved in this effect . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using Western blotting analysis , we found that the gossypol induced apoptosis may not be involved in the regulation of p 53 but possibly associated with the regulation of bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate whether PR deterioration is associated with cell turnover ( meningiomas grow much faster in vitro than in vivo ) , the relationship between expression of the apoptotic proteins Bcl 2 and Bax and PR expression was investigated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At or above this concentration , c jun N terminus kinase was activated and Bax and Bcl 2 expression were both elevated . ^^^ The elevated Bcl 2 protein might neutralize the proapoptotic effect of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In cervical tumors , inactivation of the p 53 tumor suppressor protein by the E 6 gene product of high risk HPVs and mutation of the p 53 gene in UADT is associated with alterations in the apoptotic regulatory bcl 2 and bax genes , leading to downregulation of programmed cell death ( PCD ) and increased cell proliferation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax alpha , Bcl 2 , p 53 , and caspase 3 proteins was assessed by Western blot assays . ^^^ Angiotensin 2 did not modify Bcl 2 , p 53 , and BAX mRNA in cells from the two strains of rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For this reason we investigated the relationship between expression of bcl 2 , the pro apoptotic protein Bax and MIB 1 with clinical drug resistance in Hodgkin ' s lymphoma . ^^^ Tissue sections from both groups of patients were stained for bcl 2 , Bax and MIB 1 . ^^^ In the non responding patients Reed Sternberg cells expressed bcl 2 more frequently using a cut off point of < 25 % to indicate negative cells ( P = 0 . 04 ) , whereas no differences were observed in the expression of Bax and MIB 1 . ^^^ In non responding patients the lymphocytes surrounding Reed Sternberg cells expressed bcl 2 less frequently ( P = 0 . 002 ) , using a cut off point of < 25 % , whereas no difference was observed in the expression of Bax and MIB 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blots were used to determine expression of the components involved in the initiation ( Fas , Fas Ligand , FADD ) , regulation ( Bcl 2 , Bax ) and execution ( caspase 2 and caspase 3 ) of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase 3 ( a protease which mediates apoptosis ) immunoreactivity was absent in the choroid plexus epithelial cells in the control rats and following altitude exposure ; Bcl 2 ( anti apoptotic protein ) and Bax ( pro apoptotic protein ) immunoreactivity were upregulated at 3 h 2 days following the altitude exposure when compared to the controls but not in longer surviving rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study evaluated whether induction of Bax initiates apoptosis and whether Bax overexpression enhances apoptosis induced by several chemotherapeutic agents in DLD 1 colon cancer cells , which originally express a high level of endogenous Bax protein and a low level of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic Bax is a member of the Bcl 2 family proteins , which have a key role in regulating programmed cell death . ^^^ Bax contains a BH 3 domain heterodimerizing with Bcl 2 and a hydrophobic transmembrane segment to be inserted in specified organelle membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical study was done to detect P glycoprotein , multidrug resistance associated protein , lung resistance protein , glutathione S transferase pi , p 53 , Bcl 2 , Bax , topoisomerase 1 , IIalpha and IIbeta , and Ki 67 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in Bax and Bcl 2 protein levels were determined by Western blot analyses . ^^^ Effect of Bcl 2 overexpression on induction of Bax in response to butyrate pretreatment was studied in cells transfected with Bcl 2 . ^^^ Treatment of cells over expressing Bcl 2 with BA did not show induction of Bax suggesting that higher levels of Bcl 2 can block butyrate induced increase in levels of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we found that I3C up regulates Bax , down regulates Bcl 2 and , thereby , increased the ratio of Bax to Bcl 2 favoring apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cellular markers associated with apoptosis Fas , Fas ligand ( FasL ) , Bcl 2 , and Bax were examined by immunohistochemistry . ^^^ Expression of Fas , FasL , Bcl 2 , and Bax in corneal cells showed dynamics similar to that of keratocyte depletion and endothelial changes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bax protein and Bcl 2 protein , which are known to control apoptosis , were not altered by heating . ^^^ It was also concluded that heat induced apoptosis is lessened by p 53 and that Bcl 2 and Bax are not involved in the induction of apoptosis by hyperthermia . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Real time quantitative reverse transcriptase polymerase chain reaction assays were developed and validated to quantitate expression of p 53 , the p 53 downstream effector gene p 21 , and the apoptosis related genes , bax , bcl 2 , and survivin . ^^^ There was no consistent pattern to the changes in bax , bcl 2 , or survivin after SCH 58500 treatment with or without paclitaxel between tumor types , although there were consistent responses within individual tumor lines . ^^^ The mRNA ratios for bax / bcl 2 and bax / survivin were also not informative across tumor types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was analyzed immunohistochemically with BAX and BCL 2 antibodies and by the TUNEL method . ^^^ TUNEL , BCL 2 , and BAX results did not correlate with SYT SSX fusion type . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential regulation of P 53 , c Myc , Bcl 2 , Bax and AFP protein expression , and caspase activity during 10 hydroxycamptothecin induced apoptosis in Hep G 2 cells . 10 Hydroxycamptothecin ( HCPT ) , a DNA topoisomerase 1 ( Topo 1 ) inhibitor , exhibited a remarkable apoptosis inducing effect on human hepatoma Hep G 2 cells . ^^^ We studied the effect of HCPT upon the expression of P 53 , c Myc , Bcl 2 , Bax and alpha fetoprotein ( AFP ) proteins , and caspase ( caspase 1 and caspase 3 ) activity of Hep G 2 cells . ^^^ It showed that HCPT at a dose of 0 . 1 microg / ml increased the expression of P 53 , c Myc and Bax protein , and decreased the expression of Bcl 2 and AFP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Adenosine attenuates reperfusion induced apoptotic cell death by modulating expression of Bcl 2 and Bax proteins . ^^^ This study tests the hypothesis that infarct reduction with adenosine ( Ado ) is associated with inhibition of apoptotic cell death by modulating expression of anti apoptotic Bcl 2 and pro apoptotic Bax proteins and reducing neutrophil accumulation . ^^^ Expression of Bcl 2 and Bax proteins was analyzed using Western blot assay . ^^^ Densitometrically , Ado and CGS 21680 at reperfusion significantly increased the expression ( % of normal myocardium ) of downregulated Bcl 2 from 45+ / 6 % in Control group to 78+ / 12 % * and 69+ / 10 % * , respectively , and attenuated expression of upregulated Bax from 198+ / 16 % in Control group to 148+ / 10 % * and 158+ / 12 % * , respectively . ^^^ In conclusion , the present study suggests that inhibition of apoptosis by Ado at reperfusion involves alterations in anti apoptotic Bcl 2 and pro apoptotic Bax proteins and neutrophil accumulation , primarily mediated by an adenosine A2A receptor . * P < 0 . 05 5 Control group . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decreases occurred in bcl 2 ( antiapoptotic ) , bax alpha , bad , and bok ( proapoptotic ) and cell cycle control elements ( cyclins ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 expression did not change in any of the groups , whereas Bax expression increased with ischemia reperfusion and correlated well with the degree of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Jak 3 selectively regulates Bax and Bcl 2 expression to promote T cell development . ^^^ Here we report that Jak 3 regulates T lymphopoiesis at least in part through its selective regulation of Bax and Bcl 2 . ^^^ Jak 3 deficient thymocytes express elevated levels of Bax and reduced levels of Bcl 2 relative to those in wild type littermates . ^^^ The data support the concepts that Jak kinases regulate cell survival through their selective and cell context dependent regulation of pro and antiapoptotic Bcl 2 family proteins and that Bax and Bcl 2 play distinct roles in T cell development . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of other apoptosis related genes ( bcl 2 , bcl XL , bax , bad , caspase 3 ) was not affected by light exposure or the lack of c Fos in knockout mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical study on the distribution of Bcl 2 and Bax in the central nervous system of the transgenic mice expressing a human Cu / Zn SOD mutation . ^^^ In the present study , we performed immunohistochemical studies to investigate the changes of Bcl 2 and Bax in the central nervous system of the transgenic mice expressing a human Cu / Zn SOD mutation . ^^^ Our present study demonstrated the distribution of Bcl 2 and Bax in detail using immunohistochemical methods through the central nervous system of the transgenic mice , for the first time . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Arsenic trioxide could strongly inhibit the growth of human hepatocarcinoma cells QGY 7701 and QGY 7703 with the cell cycle arrested on S phase , and induce the apoptosis of the cells with bcl 2 gene expression down regulated and bax and Fas gene expression up regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Peripheral lymphocytes of 39 SSc patients and 47 healthy control persons were studied for apoptosis , Bcl 2 and Bax levels , expression of Fas ( CD 95 ) and activation markers ( CD 25 , HLA DR ) as determined by fluorocytometry . ^^^ RESULTS : SSc lymphocytes ( mainly CD 4 ( + ) ) expressed increased amounts of Bcl 2 , while Bax was not elevated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There are either genetic or acquired conditions creating unbalance of Bax / Bcl 2 families proapoptotic and prooncogenic factors , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also transfected and expressed the human pro apoptotic gene bax and the anti apoptotic gene bcl 2 in MGH U 1 cells and compared cell survival after drug treatment with that of control cells transfected with the vector alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analyses showed that bone marrow precursors express appreciable levels of caspase 3 and caspase 9 but no or very low levels of c fms ( M CSF receptor ) and the apoptosis regulators 10 linked inhibitor of apoptosis protein ( XIAP ) , c IAP 1 , Bcl 2 and Bax . ^^^ The differentiation of BMDM is associated with a steady and gradual increase in the levels of c fms , XIAP , c IAP 1 , Bcl 2 and Bax , reaching maximal levels by day 7 . ^^^ Unlike bone marrow precursor cells , mature BMDM ( day 7 10 ) were resistant to apoptosis induced by M CSF depletion , which includes the activation of caspase 3 and caspase 9 and the degradation of XIAP , Bcl 2 and Bax proteins in the process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of Bcl 2 , Bax , and Bak in spontaneous apoptosis and proliferation in neuroendocrine lung tumors : immunohistochemical study . ^^^ Expression of Bcl 2 and Bax decreases in small cell lung carcinoma ( SCLC ) with metastases indicating attenuation of apoptosis and development of metastatic clones resistant to apoptosis induces . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because cell survival and apoptosis are regulated , in part , by the relative abundance of proteins of the Bcl 2 family , we hypothesized that lung cells dying during exposure would show increased expression of pro apoptotic members , such as Bax , whereas surviving cells would have increased expression of anti apoptotic members , such as Bcl 10 ( L ) . ^^^ The hypothesis is tested in the current study by determining which Bcl 2 genes are regulated by hyperoxia , with specific focus on correlating expression of Bax and Bcl 10 ( L ) with morphologic evidence of apoptosis or necrosis . ^^^ Adult mice exposed to greater than 95 % oxygen concentrations for 48 to 88 hours had increased whole lung mRNA levels of Bax and Bcl 10 ( L ) , no change in Bak , Bad , or Bcl 2 , and decreased levels of Bcl w and Bfl 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of the Bcl 2 family members analyzed , a significant difference was observed in the transcription of Bax mRNA , which was up regulated early after irradiation in B 6 + / + cells , but not B 6 me / me cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that Ag specific CD 8 ( + ) memory T cells contain high steady state levels of Bcl 2 , BAX : , IFN gamma , and lung Kruppel like factor ( LKLF ) , and decreased levels of p 21 and p 27 mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Association of smoking with apoptosis regulated proteins ( Bcl 2 , bax and p 53 ) in resected non small cell lung cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both anti apoptotic ( Bcl W , Bcl 10 ( L ) ) and pro apoptotic ( Bad , Bak , Bax ) members of the Bcl 2 family were expressed in developing skeletal muscle in vivo . ^^^ Loss of Bcl 2 did not affect expression of other family members , because neonatal muscles of wild type and Bcl 2 null mice had similar amounts of Bcl 10 ( L ) , Bcl W , Bad , Bak , and Bax mRNAs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In separate experiments , brains of sham operated control and HI only animals and animals subjected to HI plus C 2 ceramide or DMSO infusion were sampled 6 hours , 24 hours , and 5 days after treatments and analyzed for Bcl 2 , Bcl xl , and Bax expression ( Western blotting ) , and apoptosis ( TUNEL assay ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine Bcl 2 and Caspase family dependent apoptotic pathways in telencephalic neurons , we compared the effects of cytosine arabinoside ( AraC ) , a known neuronal apoptosis inducer , on wild type , Bcl 10 ( L ) , Bax , Caspase 9 , Caspase 3 , and p 53 deficient telencephalic neurons in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of apoptosis and Bcl 2 / Bax in the development of tubulointerstitial fibrosis during experimental obstructive nephropathy . ^^^ Tissue was immunohistochemically stained for alpha smooth muscle actin ( alpha SMA ) , proliferating cell nuclear antigen ( PCNA ) , Bcl 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Baseline multidrug resistance protein ( MRP ) , Her 2 / neu , and epidermal growth factor receptor ( EGFR ) expression , and pre and posttherapy bax and bcl 2 expression were determined by Western blot analysis . p 53 status was determined by sequencing . ^^^ Responding tumors had a higher bax / bcl 2 ratio 24 h after therapy , but the difference was only marginally significant ( 2 . 8 for responding tumors versus 1 . 1 for nonresponding tumors , P = 0 . 07 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To begin elucidating the mechanism whereby bryostatin 1 potentiated the effects of CHOP in the mouse model ; we studied the effect of bryostatin 1 on Bax , Bcl 2 , and poly ( ADP ribose ) polymerase proteins in vitro and in vivo . ^^^ Bax protein increased in a time dependent manner without any measurable change in Bcl 2 expression . ^^^ One possible mechanism may be the modulatory effects of bryostatin 1 on the Bax : Bcl 2 family of apoptosis regulatory proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The combined functions of proapoptotic Bcl 2 family members bak and bax are essential for normal development of multiple tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relationships of Bax expression to the histological grades of DCIS & IDC , and to the expression of Ki 67 , ER , p 53 , cerbB 2 & Bcl 2 are described . ^^^ The expression of Bax , Ki 67 , ER , p 53 , cerbB 2 and Bcl 2 proteins is determined immunohistochemically . ^^^ Cases were regarded positive for Bax , Bcl 2 and cerbB 2 when they showed either moderate or strong staining for these markers . ^^^ Bcl 2 is associated with good prognostic markers in both DCIS and IDC , whereas the regulation of Bax is complex and does not necessarily correlate with mutant p53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The PTPC is formed in the IM / OM contact site and contains the two most abundant IM and OM proteins , adenine nucleotide translocator ( ANT , in the IM ) and voltage dependent anion channel ( VDAC , in the OM ) , the matrix protein cyclophilin D , which can interact with ANT , as well as apoptosis regulatory proteins from the Bax / Bcl 2 family . ^^^ Ca ( 2+ ) , atractyloside , thiol oxidation , the pro apoptotic HIV 1 protein Vpr , etc . ) and is enhanced by Bax and inhibited by Bcl 2 , as well as by ADP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Progress has been made especially in the elucidation of the mechanisms of action of the Bcl 2 family members , in detail the formation of channels and their regulation in the mitochondrial membranes , conformational changes in Bax and Bak , and crosstalk of death receptor triggered apoptosis to the mitochondria by activation of Bax via Bid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) analysis did not show consistent differences in the expression of Bcl 2 and Bax between Fas sensitive and Fas resistant cell lines examined . ^^^ These findings indicated that the presence or absence of mRNA expression of DcR 3 , FAP 1 , Bcl 2 and Bax did not always correlate with relative sensitivity to Fas mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix . ^^^ Bcl 2 protein together with the pro apoptotic protein bax , are thought to function by forming homo and heterotypic dimers which control the progression to apoptosis . ^^^ In this immunohistochemical study we investigated the expression of bcl 2 and bax apoptosis related proteins in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix . ^^^ Twenty four cervical intraepithelial neoplasias grade 1 2 ( CIN I / II ) , 38 grade 3 ( CIN 3 ) , and 53 invasive squamous cell carcinomas ( ISCC ) were investigated by immunohistochemical staining for bcl 2 and bax protein . ^^^ Our results suggest that alterations of bcl 2 and bax expression may occur as a relatively early event in cervical tumorigenesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bax xL , and bcl xS in endometrial and cervical tissues . ^^^ We assessed the protein expression levels of bcl 2 , bax , bcl xL , and bcl xS in a group of 51 endometrial cancers and 8 normal samples as well as in 59 cervical neoplasms and in 15 normal cervical tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human prostate stromal ( PS ) cells were evaluated for apoptosis ( TUNEL assay ) , mRNA levels of key cell cycle regulators influencing apoptosis ( p 53 , Bax and Bcl 2 ) using quantitative RT PCR and cytotoxicity after Ad CMV p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study examines the contributions of apoptosis related proteins ( Bcl 2 , Bcl XL , Bax , and p21WAF1 / CIP1 ) in the regulation of T cell death induced by butyric acid , using p 53 knock out ( p 53 / ) and wild type ( p53+ / + ) mice . ^^^ These results suggest that butyric acid mediated apoptosis of murine T cells takes place via a pathway that is independent of p 53 , and is followed by the p 53 regulated proteins Bax and p21WAF1 / CIP1 , which lower the levels of the apoptosis antagonists Bcl 2 and Bcl XL in cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of Bcl 2 protein in breast lesions : correlation with Bax , p 53 , Rb , C erbB 2 , EGFR and proliferation indices . ^^^ Expression of bcl 2 protein was investigated and correlated with Bax , p 53 and Rb proteins , c erbB 2 , EGFR and the proliferation indices PCNA , Ki 67 and MIB 1 as well as with the conventional clinicopathological parameters in 95 cases for breast cancer tissue and 20 cases of benign hyperplastic lesions . ^^^ Bcl 2 and Bax proteins immunoreactivity was detected in normal , hyperplastic and neoplastic breast epithelium . ^^^ However , a strong positive relationship was detected between bcl 2 and Bax ( p = 0 . 008 ) , estrogen ( ER ) ( p = 0 . 007 ) and progesterone receptors ' ( PgR ) status ( p = 0 . 0003 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : The techniques used herein determined accumulation of paclitaxel / PSC 833 induced apoptotic cells with sub G 0 ( hypodiploid ) DNA content and blocked in the G2 / M phase of the cell cycle , internucleosomal DNA fragmentation , poly ( ADP ribose ) polymerase cleavage , Bcl 2 modulation and Bax up regulation , without any significant alterations in the levels of Bcl xL , CD95 / Fas or Fas L proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Independent of which bcl 2 family member was dysregulated , resistance was associated with altered regulation in the subcellular localization of bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , bcl 2 , and bax as predictors of response to radiotherapy in esophageal cancer . ^^^ We evaluated whether expression of three proteins regulating apoptosis , p 53 , bcl 2 , and bax , could predict the effect of radiotherapy in esophageal cancers . ^^^ Biopsy specimens from the 25 patients showed expression of p 53 , bcl 2 , and bax to be 48 . 0 % , 32 . 0 % , and 76 . 0 % respectively . ^^^ Effectiveness of radiotherapy was correlated with p 53 expression ( p = 0 . 047 ) , but bcl 2 and bax expression showed no relationship to effectiveness of radiotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the cytoprotective oncoprotein Bcl 2 stabilizes the mitochondrial membrane barrier function , whereas the tumor suppressor protein Bax permeabilizes mitochondrial membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the Bcl 2 family of proteins constitutes a critical checkpoint in apoptosis , acting upstream of the apoptotic machinery , we investigated the expression of six Bcl 2 homologs ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , Bak , Bad ) and one non homologous associated molecule ( Bag 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The myocardial expression of apoptosis related genes , Fas , CPP 32 , Bcl 2 and Bax , were detected with RT PCR method . ^^^ RESULTS : Myocardial expression of Fas , CPP 32 and Bcl 2 were increased in acute myocardial ischemic rats , but no significance was detected in Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of proapoptotic ( Bad , Bak , Bax ) and antiapoptotic ( Bcl 2 , Bcl XL ) genes was analyzed by quantitative RT PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of hyperbaric oxygen on the expression of proteins Bcl 2 and Bax in the gerbil hippocampus CA 1 following forebrain ischemia reperfusion ] . ^^^ METHODS : Changes of the expression of protein Bcl 2 or Bax were observed in CA 1 region of gerbil hippocampus following HBOT on ischemia reperfusion 3 days using the method of labelled streptavidin biotin ( LSAB ) immunohistochemistry staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 gene and its family genes Bax , Bcl Xl as well as Fas / Apo 1 and their clinical significance in acute leukemia . ^^^ OBJECTIVE : To study the suppressing genes of apoptosis , namely Bcl 2 , its family genes Bax , Bcl Xl , and the inducing gene of apoptosis Fas / Apo 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In both PEM and J 774 cells , mRNA expression of the anti apoptotic gene , A 1 , was selectively induced by BCG treatment as compared with other bcl 2 family members ( bcl w , bcl 2 , bcl xl , bcl xs , bax , bak , bad ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax homologs regulate the integrity of the mitochondrial outer membrane , which may also serve as a scaffold for the apoptotic machinery . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunofluorescence and Western blot analysis , we show that whilst Apaf 1 is a predominantly cytoplasmic protein , Bcl 2 , Bcl xL and Bax mostly reside on nuclear / ER and mitochondrial membranes . ^^^ This pattern of localization is maintained when the proteins are co expressed in both normal and apoptotic cells , suggesting that Bcl 2 , Bcl xL or Bax do not significantly sequester cytoplasmic Apaf 1 to intracellular membranes . ^^^ Based on these data , we propose that Apaf 1 is not a direct , physiological target of Bcl 2 , Bcl xL or Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intracellular Bcl 2 and Bax expression was measured by fluorocytometry and the rate of apoptosis was determined by the TUNEL technique and propidium iodide staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of p 53 and BAX mutations , loss of heterozygosity , p 53 and BCL 2 expression and apoptosis in basal cell carcinoma in Korean patients . ^^^ METHODS : A collection of 33 cases of BCC were screened for mutations of p 53 and BAX genes , p 53 and BCL 2 expression , loss of heterozygosity ( LOH ) and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of BAX and Bcl 2 , which regulate apoptosis , were studied by immunohistochemistry . ^^^ Furthermore , germinomas showed greater expression of BAX than did glioblastomas , while the expression of Bcl 2 was weak in both tumor types . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
GnRH Bik / Bax / Bak chimeric proteins target and kill adenocarcinoma cells ; the general use of pro apoptotic proteins of the Bcl 2 family as novel killing components of targeting chimeric proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No detectable levels of p 53 , Bax , Bcl 2 and the subunit p 20 of interleukin 1 beta converting enzyme ( ICE ) were found by Western blot , but the active subunit p 17 of 32 kDa putative cysteine protease ( CPP 32 ) was detected at 3 , 6 , and 9 h after ricin treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In MDA MB 231 cells which possess a mutant p 53 , adriamycin induced G 1 arrest and apoptosis was also associated with a concomitant up regulation of WAF1 / CIP1 mRNA while these cells did not express bax or bcl 2 messages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study relationship of decidual and villous cells apoptosis and its control gene B cell lymphoma / leukemia 2 ( bcl 2 ) , bcl 2 associated 10 protein ( bax ) with medical abortion . ^^^ The cell cycles of DNA of the decidual and villous cells stained with Propidium Iodide ( PI ) were analyzed and bcl 2 and bax gene protein labeled with immuno fluorescence were detected by flow cytometry . ^^^ Decidual bcl 2 / bax ratio were 1 . 4 + / 0 . 7 , 0 . 9 + / 0 . 3 , 1 . 4 + / 0 . 7 and villous bcl 2 / bax ratio were 1 . 0 + / 0 . 4 , 0 . 8 + / 0 . 3 , 1 . 4 + / 0 . 6 in the 3 groups respectively . ^^^ Both decidual and villous bcl 2 / bax ratio of exp . 2 group was significantly lower than that of control ( P < 0 . 05 0 . 01 ) which was in consistent with apoptosis . ^^^ The descent of bcl 2 / bax ratio could be one of the factors of inducing decidual and villous apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Up regulation of the CDK inhibitor p 27 was observed , which may lead to G2 / M cell accumulation , but no substantial changes in expression of Bcl 2 and Bax proteins were found . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and Bax expression was detected by confocal laser scanning microscope . ^^^ RESULTS : The morphological and biochemical changes characteristic of apoptosis were partially restored after treatment with sodium ferulate , Immunofluorescence assay showed a decrease in BCL 2 and an increase in Bax oncogene proteins in lymphocytes treated with H2O2 and an improvement of cell apoptosis was found after sodium ferulate treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Members of the Bcl 2 family of proteins are important regulators of programmed cell death pathways with individual members that can suppress ( eg Bcl 2 , Bcl XL ) or promote ( eg Bax , Bad ) apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , EGF did not affect the levels of several proteins that regulate apoptotic pathways , including bcl 2 , bclXL , bax and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In response to oncogene activation , p 53 mediates apoptosis through a linear pathway involving bax transactivation , Bax translocation from the cytosol to membranes , cytochrome c release from mitochondria , and caspase 9 activation , followed by the activation of caspase 3 , 6 , and 7 . p 53 mediated apoptosis can be blocked at multiple death checkpoints , by inhibiting p 53 activity directly , by Bcl 2 family members regulating mitochondrial function , by E1B 19K blocking caspase 9 activation , and by caspase inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify the mechanism of ischemic tolerance induced by HBO , we investigated the effect of HBO on immunoreactivity to Bcl 2 and Bax , apoptosis regulating protein , or Mn SOD , a radical scavenging system , in the CA 1 sector of the gerbil hippocampus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Tax in a human T cell line , Jurkat , induced the expression of the Bcl xL gene , but did not significantly affect the expression of the other apoptosis related genes , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Five pancreatic cell lines were investigated for the expression of FasL / Fas , DcR 3 , DR 4 , DR5 / TRAIL , DcR 1 , DcR 2 , and other death pathways related molecules such as Bax , bcl xL , bcl 2 , FADD , and caspase 3 by flow cytometry , immunoblotting , and RT / PCR , both semiquantitative and real time ( TaqMan ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pancreas was dissected and immunohistochemistry was performed for analysis of the expression of the apoptosis promoting protein bax and the apoptosis inhibiting protein bcl 2 , and for BrdU and CCK A receptor localization . ^^^ Immunohistochemistry revealed decreased tissue expression of bax but unaffected expression of bcl 2 . ^^^ Immunohistochemistry showed increased expression of bax , whereas bcl 2 remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The importance of biological factors ( bcl 2 , bax , p 53 , PCNA , MI , HPV and angiogenesis ) in invasive cervical cancer . ^^^ OBJECTIVE : The present study was designed to analyse the relationship between apoptosis related proteins ( bcl 2 and bax ) , tumour suppressor protein p 53 , proliferation markers ( PCNA and mitotic index ) , human papillomavirus ( HPV ) and angiogenesis in cervical cancer and their impact on clinical outcome . ^^^ STUDY DESIGN : Tumours from 111 patients were assessed by immunohistochemistry for the expression of bcl 2 , bax , p 53 and PCNA , by PCR for the presence of HPV DNA , for the quantification of the mitotic index and the microvessel density ( CD 31 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The latter retained an increased susceptibility to tRA induced apoptosis compared with normal media SMCs . tRA induced apoptosis associated with an increased ratio of bax to bcl 2 by bax overexpression and cleavage of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : For immunohistochemistry , tissue sections of 32 patients were treated with an antigen retrieval METHOD : Primary antibodies against the apoptosis related proteins , bcl 2 , bcl 10 , bax , and bak were applied . ^^^ Most of the outer layer cells were predominantly stained by the bcl 2 antibody , while most of the inner layer cells were stained by antibodies against the apoptosis modulating proteins , bax and bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The deficiency of PAI 1 in the medium caused a significant reduction in Bcl 2 and Bcl XL mRNAs and an increase in Bcl XS and Bax mRNAs in PC 12 neurons at 3 h . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Fas , proapoptotic proteins , were detected homogeneously throughout both ventricles in the neonate , while Bcl 2 , an antiapoptotic protein , was not detectable . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Western blot analysis showed the apoptosis of the granulosa cells induced by palmitic acid to be accompanied by the down regulation of an apoptosis inhibitor , Bcl 2 , and the up regulation of an apoptosis effector , Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The adenovirus Bcl 2 homologue , E1B 19K , inhibits TNF alpha mediated apoptosis , interacts with Bax , and blocked the formation of the 500 kDa Bax complex . ^^^ Exposure of the Bax amino terminus facilitates E1B 19K Bax binding , which prevented exposure of the carboxy terminal Bax Bcl 2 homology region 2 epitope . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overall , the current study demonstrated that oxidative stress induced apoptosis in human aortic endothelial cells through the downregulation of bcl 2 , translocation of bax , and upregulation of p 53 , probably through NF kappaB activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bcl 10 , and Bax proteins in cultured fibroblasts was studied by Western blotting . ^^^ Up regulation of the Bax : Bcl 2 ratio in SSc fibroblasts by Bcl 2 antisense oligonucleotides restored their susceptibility to Fas mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Can expression of apoptosis genes , bcl 2 and bax , predict survival and responsiveness to chemotherapy in node negative breast cancer patients . ^^^ The objective of this study was to test the hypothesis that the expression of apoptosis genes , bcl 2 and bax , predicts survival and responsiveness to chemotherapy in node negative breast cancer patients . ^^^ METHODS : One hundred thirty premenopausal women with primary breast carcinoma were studied for the expression of bcl 2 and bax genes . ^^^ The relationship between the expression of bcl 2 and bax proteins and a series of markers of known prognostic value [ such as tumor size , nuclear grade , receptors of the steroid hormones estrogen ( ER ) and progesterone ( PgR ) ] . ^^^ RESULTS : Sixty ( 46 % ) and sixty four ( 49 % ) breast cancer cases were found positive for bcl 2 and bax , respectively , as indicated by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Mcl 1 expression were downregulated in both flavopiridol and fludarabine induced apoptotic cells , but the increase in Bax expression that accompanied fludarabine induced apoptosis was not evident in flavopiridol treated cells . ^^^ In addition , Bcl 2 : Bax ratios were not predictive of flavopiridol cytotoxicity ( P = 0 . 82 ) , whereas they were highly predictive of in vitro responsiveness to fludarabine ( P = 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also investigated intracellular levels of Bcl 2 , bcl xl and bax in CD 4 ( + ) and CD 8 ( + ) CVI T cells , as well as the bax / Bcl 2 ratio , using flow cytometry techniques but could not detect any differences between CVI and normal subjects . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ATF 3 strongly downregulated CRE dependent transcription , while ATF 3 did not affect the expression levels of Bcl 2 , Bcl 10 , or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because IL 6 has been reported to induce apoptosis by downregulating expression of Bcl 2 , we analyzed the expression of apoptosis related Bcl 2 , Bax , and cytochrome C by Western blot analysis . ^^^ Bax protein increased in a time dependent manner , and the ratio of Bax to Bcl 2 rose markedly after PDT in LLC IL 6 cells . ^^^ These results suggest that the increased sensitivity of LLC IL 6 cells to PDT induced cytotoxicity results from the high ratio of Bax to Bcl 2 in the IL 6 dependent apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In both cell populations we did not observe differences in the expression patterns of COX 2 , Bcl 2 , Bcl ( XL ) , Bax , and Akt / PKB activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis regulatory protein bcl 2 and bax in endometrium of polycystic ovary syndrome ] . ^^^ OBJECTIVE : To study the expression and implication of apoptosis regulatory protein bcl 2 and bax in endometrium of polycystic ovary syndrome ( PCOS ) . ^^^ METHODS : The apoptosis regulatory protein expression of bcl 2 and bax protein in endometrium were investigated by immunohistochemical method in 18 PCOS and 27 normal subjects proliferative phase 10 and secretory phase 17 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we report that a toxic regimen of METH did cause significant increases in the pro death Bcl 2 family genes BAD , BAX , and BID . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis included immunostaining for Fas , FasL , Bcl 2 , and Bax ; in situ apoptosis detection ( TUNEL assay ) ; RT PCR amplification for IFN gamma ; and measurement of ocular nitrite levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overall , results support the hypothesis that BH 3 peptide releases cytochrome c by a Bax dependent process that is independent of the mitochondrial permeability transition pore but regulated by Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As revealed by immunoblotting , bax protein was expressed in HUVECs treated with 1 mg / ml etoposide whereas bcl 2 protein disappeared . ^^^ These findings provide evidence of relationships between endothelial toxicity of anticancer drugs and the key role of bcl 2 for resistance of endothelium cells toward apoptosis ; moreover lack of p 53 and bax in quiescent cells contributes to resistance of endothelial cells to DNA damaging agents . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A panel of human B lineage lymphoma cell lines differing in cancer drug resistance status and Bcl 2 / Bax expression were used to study the contribution of mitochondrial based perturbations and regulation in differential induction of apoptosis . ^^^ The cancer drug resistant cell lines EW 36 and CA 46 , overexpressing Bcl 2 and deficient in Bax , respectively , were both resistant to mitochondrial toxicant induced cleavage of poly ( ADP ribose ) polymerase ( PARP ) and morphologically detectable apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced in the IL 3 dependent murine pro B lymphocytic ( FL5 . 12 ) cell line by the 5 lipoxygenase activating protein inhibitor MK 886 is accompanied by the rapid loss of the anti apoptotic bcl 10 ( L ) and bcl 2 , but not the proapoptotic bax proteins ( Datta et al . , J . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We therefore assessed expression levels of the anti apoptotic proteins BCL 2 and BCL XL and the pro apoptotic proteins BAX and BCL XS in T / null cell ALCL using immunohistochemical methods and correlated the findings with ALK expression and apoptotic rate ( AR ) , the latter assessed by a modified Tdt mediated dUTP nick end labeling assay . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , we have demonstrated for the first time that a proapoptotic member of the Bcl 2 family , Bax , is required for TRAIL mediated apoptosis in HCT 116 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of prenatal exposure to ethanol on the expression of bcl 2 , bax and caspase 3 in the developing rat cerebral cortex and thalamus . ^^^ Effectors such as bcl 2 , bax , and caspase 3 can determine whether a neuron survives or dies . ^^^ Samples were collected from fetuses ( gestational day ( G ) 16 and G 19 ) and pups ( postnatal day ( P ) 0 through P 30 ) and examined for bcl 2 , bax , or caspase 3 expression using a quantitative immunoblotting procedure . ^^^ Prenatal exposure to ethanol reduced cortical bcl 2 expression , but not bax expression on P 6 . ^^^ Hence , the bcl 2 / bax ratio was lower in Et treated rats than in controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also checked the levels of BAX / BCl 2 , caspases ( for e . g . , 9 , 7 , 3 , 8 ) , PARP , cytochrome c and Apaf 1 . ^^^ Moreover , the increase of apoptosis in vitro is associated with increased and decreased expression of apoptotic protein BAX in sense clones ( SNB 19 ) and antisense clones ( Hs 683 ) respectively , when compared to controls and vice versa with Bcl 2 the anti apoptotic protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To define the mechanism of the cytokine production enhancements and inhibitions by SOM and SP , we examined the expression of apoptosis modulator , p 53 , Bcl 2 , Bax , inducible nitric oxide synthase ( iNOS ) , Fas , caspase 8 and nitric oxide ( NO ) in thioglycolate elicited peritoneal macrophages . ^^^ These data suggest that apoptosis by SOM may occur by a Bax and NO independent p 53 accumulation , and through Fas and caspase 8 activation pathways , and that the inducible expression of Bcl 2 and NO production by SP may contribute to prevent the signals of apoptosis by Bax , and via Fas and caspase 8 activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BH 3 only Bcl 2 family members are coordinately regulated by the JNK pathway and require Bax to induce apoptosis in neurons . ^^^ Here we show that the BH 3 only Bcl 2 family members , Dp5 / Hrk and Bim , are induced upstream of the Bax checkpoint in neuronal apoptosis in a manner that shows significant dependence on JNK signaling . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although expression of bax mRNA and its protein product were enhanced , while bcl 2 protein was decreased by CDDP in COLO 357 cells , expression of mRNA of the bcl 2 family and protein product were not influenced by CDDP in T3M4 cells . ^^^ Increased expression of bax and reduced expression of bcl 2 are involved in the growth inhibitory effect of CDDP on pancreatic cancer cells with wild type p 53 gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Appearance of shortened Bcl 2 and Bax proteins and lack of evidence for apoptosis in rat forebrain after severe experimental traumatic brain injury . ^^^ To investigate whether apoptosis plays a role in traumatic brain injury ( TBI ) , we examined the expression of Bcl 2 and Bax proteins and the release of mitochondrial cytochrome c in rat brains using Western blot analysis . ^^^ Treatment of animals with an antioxidant N acetylcysteine administered ip greatly diminished the levels of shortened Bcl 2 and Bax proteins . ^^^ These findings suggest that the induction of shortened Bcl 2 and Bax proteins in rat brains may be associated with reactive oxygen species generated after TBI . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A possible role of Bcl 2 and Bax in hypoxia induced apoptosis in different cell types has been proposed . ^^^ The induction of apoptosis in hypoxic hearts correlated with a significant decrease of Bcl 2 protein level , whereas Bax protein expression was increased . ^^^ However , Bcl 2 and Bax protein levels essentially remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bcl 2 , and NF kappaB expression in sanguinarine induced bimodal cell death . ^^^ The apoptosis related proteins Bax , Bcl 2 , and NF kappaB were analyzed in sanguinarine induced apoptosis and blister cell death ( BCD ) of K 562 erythroleukemia cells and in sanguinarine treated high Bcl 2 expressing JM 1 pre B lymphoblastic cells , utilizing immunofluorescence flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These effects include the following : ( 1 ) induction of cell apoptosis and cell cycle modulation ; ( 2 ) inhibition of cell proliferation ; ( 3 ) downregulation of bcl 2 , bcl 6 , proliferating cell nuclear antigen , and prostate specific antigen proteins ; ( 4 ) downregulation of androgen receptor ( AR ) ; and ( 5 ) upregulation of p 53 , bax , and p 21 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It is known that Bax , caspase 3 , and p 53 proteins are death promoting factors , whereas Bcl 2 protein is a death antagonist . ^^^ We immunohistochemically examined the expression of Bax and apoptosis related proteins such as caspase 3 , p 53 , and Bcl 2 in 76 patients with human esophageal squamous cell carcinoma ( SCC ) including dysplasia to determine the relationship of expression of each protein to tumor behavior and patients ' prognosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In 204 cases the operative material was examined taking into account : 1 . the age , sex , size of tumor , macroscopic features , stage , angioinvasion and perineural invasion , 2 . cancer response to the treatment , 3 . immunohistochemical factors being the expression of p 53 , MIB 1 , bcl 2 , bax . ^^^ In operative material after radio chemotherapy a statistically significant dependence occurred among all immunohistochemical markers ; regression of the tumor mass less than 2 / 3 correlated with the low expression of p 53 , MIB 1 , bax and bcl 2 . 4 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting analysis and flow cytometric studies revealed that HGF inhibited doxorubicin and etoposide induced decreases in the levels of the anti apoptotic proteins Bcl 10 ( L ) , and to a lesser extent Bcl 2 , without inducing changes in the pro apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bax and bcl xl in human gliomas : a re appraisal . ^^^ We have analyzed the expression of the anti apoptotic proteins bcl 2 , bcl xl and that of bax , a pro apoptotic protein , in human WHO grade 2 astrocytomas ( LGA ) and WHO grade 4 glioblastoma multiforme ( GBM ) . ^^^ IHC indicated that the expression of bcl 2 was inversely correlated to the grade of the tumors ( i . e more cells were bcl 2 positive in LGA than in GBM ) while the expression of bax was unaffected by the grade of the tumor . ^^^ In contrast , immunoblots revealed a parallel increase in the expression of bcl 2 and bax from the low to high grade tumor , suggesting a co regulation of the expression of these two proteins during tumoral progression . ^^^ Confocal analyses provide us with another possible level of complexicity in the regulation of apoptosis in these tumors , as these markers exhibited different subcellular localizations : bcl 2 was strictly associated with mitochondria and bcl xl was present in both cytosolic and mitochondrial compartments while bax was found essentially in the cytosol of the tumoral cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Modulation of angelica sinensis polysaccharides on the expression of nitric oxide synthase and Bax , Bcl 2 in liver of immunological liver injured mice ] . ^^^ OBJECTIVE : To investigate the modulation of Angelica Sinensis Polysaccharides on the expression of nitric oxide synthase and Bax , Bcl 2 in the liver of immunological liver injured mice . ^^^ The effects of Angelica Sinensis Polysaccharides on the expression of nitric oxide synthase , Bcl 2 , and Bax gene were assessed by immunohistochemistry , and the nitric oxide ( NO ) production , alanine transaminase ( ALT ) activity , glutathione S transferase ( GST ) activity in serum were determined . ^^^ The contents of iNOS and Bax were 17 . 8 times ( P < 0 . 001 ) and 1 . 31 times ( P < 0 . 05 ) of control values , respectively . cNOS expression was not obviously changed , and no Bcl 2 expression was found in immunological liver injured mice . ^^^ Angelica Sinensis Polysaccharides of 30mg / kg could reduce the levels of NO , sALT , and sGST by 24 . 6 % , 40 . 8 % , and 18 . 4 % , respectively ; the expression of iNOS and Bax decrease by 84 . 2 % and 37 . 1 % ; and the expression of cNOS , Bcl 2 increase by 66 . 8 % and 3 . 38 times , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although Fas mediated apoptosis involved changes in Bcl 2 , Bax , and Bad regulatory proteins , such alterations were not observed with Tat . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : To assess the relation between growth factors , growth factor receptors , p 53 , bcl 2 and bax expression , and the rate of apoptosis in invasive breast cancer patients . ^^^ Besides , the expression patterns of the bcl 2 , bax and p 53 gene products were investigated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bax / Bcl 2 ratio determines the susceptibility of human melanoma cells to CD95 / Fas mediated apoptosis . ^^^ The assignment of Bax / Bcl 2 ratios by quantitative Western blotting in 11 melanoma cell populations revealed a relation to the susceptibility to CD 95 mediated apoptosis . ^^^ We could show that a low Bax / Bcl 2 ratio was characteristic for resistant cells and a high Bax / Bcl 2 ratio was characteristic for sensitive cells . ^^^ The Bax / Bcl 2 ratio was also in clear correlation with sensitivity to another cell death inducer , N acetylsphingosine . ^^^ Furthermore , Bcl 2 overexpression abolished apoptosis triggered by both apoptotic stimuli , confirming the critical role of the Bax / Bcl 2 ratio as a rheostat that determines the susceptibility to apoptosis in melanoma cells by regulating mitochondrial function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Application of 5 LO or 5 LO activating protein directed inhibitors , but not a cyclooxygenase inhibitor , reduced growth , increased apoptosis , down regulated bcl 2 , up regulated bax , and increased G 1 arrest . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study examined ( a ) the seroprevalence of HP , its cytotoxic protein , CagA , and cytokines ( tumor necrosis factor alpha , interleukins 1beta and 8 ) in 80 patients with colorectal cancers , before and after the removal of tumor , compared with 160 age and gender matched controls ; ( b ) the gene expression of gastrin and its receptors ( CCKB R ) in the cancer tissue , ( c ) the plasma levels and tumor tissue contents of gastrin , and ( d ) the mRNA expression of COX 1 , COX 2 , and apoptotic proteins ( Bax and Bcl 2 ) in cancer tissue and intact colonic mucosa . ^^^ Anti HP IgG , anti CagA IgG seroprevalence , and cytokine levels were analyzed by enzyme linked immunosorbent assay tests ; gene expressions of gastrin , CCKB R , COX 1 , COX 2 , Bax , and Bcl 2 by reverse transcriptase polymerase chain reaction ; and gastrin by radioimmunoassay . ^^^ We conclude that colon adenocarcinoma and its resection margin overexpress gastrin , its receptors , CCKB R , and COX 2 , and that HP infection may contribute to colonic cancerogenesis via overexpression of gastrin and COX 2 , which may account for the stimulation of the tumor growth and the reduction in apoptosis as documented by enhanced mRNA expression of anti apoptotic Bcl 2 over proapoptotic Bax proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the induction of chemoresistance , the appearance of a functional P glycoprotein ( P gp ) , in addition to the expression of anti apoptotic Bcl 2 , Bcl XL and pro apoptotic Bax proteins was assessed . ^^^ Parental cells which are sensitive to dox , have no P gp activity and express Bcl 2 and Bax . ^^^ In addition , the synthesis of Bcl 2 appeared to be replaced by Bcl XL while that of Bax remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of the apoptosis regulating proteins ( BCL 2 , BAK , and BAX ) were determined by Western blotting . ^^^ RESULTS : PS 341 decreased BCL 2 , without effect on BAX or BAK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , huperzine A partly reversed the down regulation of anti apoptotic Bcl 2 and the up regulation of pro apoptotic Bax and P 53 proteins and reduced the apoptosis that normally followed beta amyloid injection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , estrogen receptors , progesterone receptors , and apoptosis associated proteins , Fas , Fas ligand , and Bax were expressed in both endometriosis and carcinoma tissues of the tumor , whereas Bcl 2 was not expressed in either tissue of the tumor . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AdL540Q or Ad 1 536 infection also inhibited GH ( 4 ) cell growth and induced apoptosis , increasing the expression of the proapoptotic Bax protein and decreasing the expression of antiapoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , U 69593 increased the content of the anti apoptotic members of the Bcl 2 family of proteins , the Bcl 2 and Bcl 10 ( L ) , whereas it had no significant effect on the apoptosis promoting homologues Bax , Bcl 10 ( S ) and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of p 53 , bax and bcl 2 gene expression in patients with laryngeal carcinoma . ^^^ AIM : This study was designed to examine the prognostic significance of the coexpression of three genes ( bax , bcl 2 and p 53 ) which play a critical role in the apoptotic mechanisms in patients with squamous cell laryngeal carcinoma . ^^^ MATERIALS AND METHODS : The immunohistochemical expression of bcl 2 , bax and p 53 genes was retrospectively examined in 38 patients with squamous cell laryngeal carcinoma and in five controls ( necrotomic tissue ) . ^^^ Patients who were bcl 2 ( ) / p53 ( ) ( n=25 ) or bax ( + ) / bcl 2 ( ) ( n=13 ) had a significantly worse overall survival ( P=0 . 0305 and P=0 . 0482 , respectively ) . ^^^ Similarly , patients who were bax ( + ) / bcl 2 ( ) / p53 ( ) ( n=11 ) also had a worse 5 year survival compared with the rest of the group ( P=0 . 0088 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR studies showed up regulated p 53 and Bax but lowered Bcl 2 mRNA levels with H2O2 treatment . ^^^ Preincubation with HupA ( 1 microM ) significantly prevented the cells from apoptosis , attenuated H2O2 induced over expression of Bax and p 53 , and rehabilitated the level of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The neurons died by a caspase dependent mechanism after inhibition of PI 3 kinase , and were also killed by antisense Bcl xL and antisense Bcl 2 or by overexpression of Bcl xS , Bad , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In mutant K ras pancreatic carcinoma cells , programmed cell death correlates with expression and an increase , respectively , in MDA 7 and BAX proteins and increases in the ratio of BAX to BCL 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax and interleukin 1beta converting enzyme increased in the mastitic tissue at 24 h and 72 h postinfection , whereas Bcl 2 expression decreased at 24 h but did not differ significantly from the control at 72 h postinfection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a pro apoptotic member of the Bcl 2 family , is a cytosolic protein that inserts into mitochondrial membranes upon induction of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using mid gestation human jejunum and colon organotypic cultures , we analyzed the impact of growth factors ( namely insulin ; 10 microg / ml ) and pharmacological compounds that inhibit signal transduction molecules / pathways ( namely tyrosine kinases , Fak , P 13 K / Akt , and MEK / Erk ) on cell survival and Bcl 2 homolog expression ( anti apoptotic : Bcl 2 , Bcl 10 ( L ) , Mcl 1 ; pro apoptotic : Bax , Bak , Bad ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study the relationship between exposure to 60 Hz magnetic fields ( MFs ) and breast cancer , cell cycle distribution , apoptosis , and the expression of related proteins ( p 21 , Bax , and Bcl 2 ) were determined in MCF 7 cells following exposure to magnetic fields ( 60 Hz , 5 mT ) alone or in combination with 10 rays . ^^^ Similarly , 60 Hz MFs did not induce apoptosis or the expression of Bax and Bcl 2 , two proteins related to apoptosis . ^^^ However , exposure of cells to 60 Hz MFs for 24 h after irradiation by 10 rays ( 12 Gy ) significantly decreased apoptosis and Bax expression but increased Bcl 2 expression . ^^^ The effects of exposure to 60 Hz MFs on 10 ray induced apoptosis and Bax and Bcl 2 expressions were not observed at 72 h . ^^^ These data suggest that exposure to 60 Hz MFs has no effects on the growth of MCF 7 cells , but it might transiently suppress 10 ray induced apoptosis through increasing the Bcl 2 / Bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis was used to measure the expression of the antiapoptotic protein Bcl 2 and apoptotic protein Bax . ^^^ These changes were associated with a 27 % decrease in Bcl 2 protein , a 42 % increase in Bax and a 57 % reduction in the ratio of Bcl 2 / Bax . ^^^ This effect was associated with an increase in oxidative stress , up regulation of Bax protein and down regulation of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Specifically , this report examined expression of apoptotic promoters Bax and Bad and apoptotic inhibitors Bcl 2 and Bcl 10 ( all members of the Bcl 2 protein family ) . ^^^ Expression of Bcl 2 , Bcl 10 , Bax , and Bad and the extent of apoptosis were determined by flow cytometric analysis of freshly isolated cells and cells cultured with TGF beta ( 1 ) and FL effectors . ^^^ Thus , addition of TGF beta 1 further reduced the already low Bcl 2 : Bax ratio in CD 34 ( + ) CD 38 ( / low ) cells . ^^^ This suggests that the preferential induction of apoptosis in primitive cells by TGF beta ( 1 ) may be due to its further reduction of the Bcl 2 : Bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : Bcl 2 proto oncogene , an inhibitor of apoptosis and Bax proto oncogene , an inducer of apoptosis play critical roles in the molecular circuit controlling apoptosis in cardiac muscle . ^^^ The ratio of Bax to Bcl 2 proto oncogene determines survival or death after an apoptotic stimulus . ^^^ We speculated that susceptibility of myocytes to apoptosis determined as the Bax / Bcl 2 ratio might vary with the severity of heart failure . ^^^ METHODS AND RESULTS : We studied immunohistochemically 108 endomyocardial biopsy specimens from 30 patients with idiopathic dilated cardiomyopathy ( mild heart failure , n=14 ; moderate or severe heart failure , n=16 ) with the use of Bcl 2 and Bax monoclonal antibodies . ^^^ Patients with mild heart failure showed significantly increased Bax / Bcl 2 ratio than the patients with advanced heart failure ( 1 . 59+ / 1 . 26 vs . 0 . 34+ / 0 . 43 , P=0 . 002 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pro apoptotic proteins bax and bak were overexpressed after treatment , while the protein level of bcl 2 remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Initiation of apoptosis in LA treated MCF10A cells was detected by mitochondrial localization of the Bax apoptotic protein , which was prevented by overexpression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Curcumin induced cell death was mainly due to apoptosis in which a prominent downregulation of Bcl 2 and upregulation of Bax were involved . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , gene expression of cyclooxygenase 1 ( COX 1 ) and COX 2 and apoptosis related proteins such as Bax and Bcl 2 are discussed . ^^^ Gene expression of bcl 2 was detected in all , while bax only in about 50 % of GC samples . ^^^ CONCLUSIONS : Infection with H . pylori , especially that expressing CagA positivity , is primum movens in developing GC and MALT lymphoma and the upregulation of growth factors , particularly of gastrin , and COX 2 and dysregulation of the Bax / Bcl 2 system seem to contribute to gastric cancerogenesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , a pro apoptotic Bcl 2 family protein , Bax , but not Bad or Bid , was translocated from cytosol to mitochondria in TCR stimulated thymocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transient expression of wild type or mitochondrially targeted Bcl 2 induces apoptosis , whereas transient expression of endoplasmic reticulum targeted Bcl 2 is protective against Bax induced cell death . ^^^ Additionally , we find that transiently expressed ER targeted Bcl 2 inhibits cell death induced by Bax overexpression . ^^^ Furthermore , the ability of ER targeted Bcl 2 to protect against Bax induced apoptosis suggests that the ER localization of Bcl 2 may play an important role in its protective function . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis and immunohistochemistry were performed to examine the expression of p 53 , proliferating cell nuclear antigen , Bax , Bcl 2 , p21WAF1 / Cip1 and Gadd 45 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The earliest detectable effect of photodamage is the selective loss of the antiapoptotic protein bcl 2 leaving the proapoptotic protein bax undamaged . ^^^ Based on results reported here we propose that the apoptotic response to these photosensitizers is derived from selective photodamage to the antiapoptotic protein bcl 2 while leaving the proapoptotic protein bax unaffected . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses revealed an increased bax / bcl 2 ratio in MCF 7 CM treated FHSO 6 cells . ^^^ These data indicate that FasLigand secreted by BCCs induces apoptosis and necrosis of human preosteoblastic stromal cells through caspase cascade modulated by the bax and bcl 2 protein level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot technique was used for the determination of proapoptotic protein ( bax ) , antiapoptotic protein ( bcl 2 ) , apoptotic fragments of poly ADP ribose polymerase , and ICAM 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , phosphatase inhibitor okadaic acid induces apoptosis in U 937 cells via a mechanism that appears to involve caspase 3 activation , but not modulation of Bcl 2 , Bax , and Bcl 10 ( L ) expression levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Without cisplatin treatment , Bcl 2 and Bax were expressed in H460 / CIS cells , but not in parental cell . ^^^ Our data suggested that p 53 , Bax and Bcl 2 were up regulated in H460 / CIS cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Programmed cell death ( PCD ) in many systems is controlled by relative amounts of the apoptosis regulating proteins Bax and Bcl 2 through homo or heterodimerization . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic value of p 53 , p21 / WAF1 , Bcl 2 , Bax , Bak and Ki 67 immunoreactivity in pT 1 G3 urothelial bladder carcinomas . pT 1 G3 bladder carcinomas are heterogeneous with respect to tumor recurrence and progression . ^^^ Our goal was to determine the prognostic value of p 53 , p21 / WAF1 , Bcl 2 , Bax , Bak , and Ki 67 immunoreactivity in these tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of regulating molecules was analyzed by using western blotting for p 53 , Bcl 2 , Bax , Bcl XL , Bcl XS , and p 21 ( WAF1 / CIP1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate the mechanisms of neuronal cell death , hippocampal sections of 20 patients dying from bacterial meningitis were investigated by immunohistochemistry using antibodies against the proform of caspase 3 and the active enzyme , bcl 2 , bax and p 53 . ^^^ The median density of immunoreactive neurons was 2 . 3 / mm2 ( procaspase 3 ) , 0 . 9 / mm2 ( activated caspase 3 ) , 1 . 8 / mm2 ( bcl 2 ) , 1 . 1 / mm2 ( bax ) and 0 . 4 / mm2 ( p 53 ) . 80 % of neurons immunoreactive for active caspase 3 had an apoptotic morphology , whereas only 10 % of all procaspase 3 stained neurons showed signs of apoptosis . ^^^ Neuronal expression of caspase 3 , bcl 2 and bax suggests an involvement of these proteins in neuronal death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Herein , a focal point is the mitochondrial control of specific death enzymes so called caspases by members of the pro apoptotic Bax and BH 3 subfamily or the anti apoptotic Bcl 2 subfamily . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While bcl 2 , bax , NFkappaB and p 53 gene expression were spontaneously upregulated , bcl xL and p21WAF1 gene expression decreased and IkappaB remained unchanged during the activation process in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased expressions of Bax and Bad were detected in HAECs incubated for 24 h with gly ox HDL , but gly ox HDL failed to interfere with the expression of Bcl 2 and Bcl 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND / AIM : EAT / mcl 1 ( EAT ) , an immediate early gene , functions in a similar way to bcl 2 in neutralising Bax mediated cytotoxicity , suggesting that EAT is a blocker of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bcl 10 , and Bax expression by immunohistochemistry in inclusion body myositis : a study of 27 cases . ^^^ CONTEXT : Bcl 2 , Bcl 10 , and Bax are among the variety of proteins that have been described as being involved in the regulation of apoptotic cell death . ^^^ Bcl 2 and Bcl 10 ( L ) inhibit apoptosis , and Bax is proapoptotic . ^^^ OBJECTIVE : To evaluate the expression of Bcl 2 , Bcl 10 , and Bax in inclusion body myositis ( IBM ) . ^^^ We examined muscle specimens from 27 patients ( 17 men , 10 women ) with IBM to evaluate Bcl 2 , Bcl 10 , and Bax expression by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) alpha mediated death signaling induces oligomerization of proapoptotic Bcl 2 family member Bax into a high molecular mass protein complex in mitochondrial membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
GFP , results in highly specific cytotoxicity in LNCaP cells , and that consequent overexpression of Bax in prostate carcinoma , even in the context of high levels of Bcl 2 protein , resulted in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : In the current study , the expression of the apoptosis related molecules p 53 , Bax , Bcl 2 , Fas ( CD 95 ) , Fas Ligand and perforin was examined in 7 patients with a sarcoma of the pulmonary artery . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HuH 7 cells do not express Bcl 2 ; however , down regulation of Bcl xL expression preceded activation of the caspase cascade in GGOH treated HuH 7 cells , while Bax expression was not changed by GGOH treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The potential role of bcl 2 , bax , and Ki 67 expression in thymus of patients with myasthenia gravis , and their correlation with clinicopathologic parameters . ^^^ OBJECTIVE : The aim of this study was to evaluate bcl 2 , bax ( apoptotic oncoproteins ) , and Ki 67 ( cell proliferation marker ) expression in thymus of patients with myasthenia gravis ( MG ) and to determine the potential correlation with clinicopathologic parameters . ^^^ On paraffin sections , the streptavidin biotin technique , using antibodies to bcl 2 , bax , and Ki 67 , was employed , and in situ hybridization with digoxigenin labeled probes to bcl 2 and bax was performed . ^^^ CONCLUSIONS : In patients with MG who underwent MMT , bcl 2 , bax , and Ki 67 expression correlates positively or reversibly with the microscopic findings of thymus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and the expression of Bax and Bcl 2 in hyperplasia and adenocarcinoma of the uterine endometrium . ^^^ This study was designed to investigate the occurrence of apoptosis and the expression of Bcl 2 and Bax proteins in endometrial tumours of corpus uteri . ^^^ The expression of Bcl 2 and Bax proteins was also investigated using immunohistochemical staining with appropriate antibodies . ^^^ Neoplastic cells in adenocarcinoma might show apoptosis in association with a decreased expression of Bcl 2 and an increased expression of Bax . ^^^ Therefore , the frequency of apoptosis and the expression of Bcl 2 and Bax might be correlated with carcinogenesis in the uterine endometrium of humans . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We investigated the expression of Bcl 2 , Bcl 10 , and Bax using immunohistochemistry in 86 ESCCs , and scored the expression by the weighted score . ^^^ The inverse relationship between Bcl 2 and Bcl 10 expression was detected ( P = 0 . 001 ) , while the positive one between Bcl 10 and Bax expression was detected ( P = 0 . 014 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 , BCL 10 ( L ) sequester BH 3 domain only molecules preventing BAX and BAK mediated mitochondrial apoptosis . ^^^ Comparison of wild type versus mutant BCL 2 , BCL 10 ( L ) indicates these antiapoptotics sequester BH 3 domain only molecules in stable mitochondrial complexes , preventing the activation of BAX , BAK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Fas , Fas ligand ( Fas L ) , ERK , MEK , Bcl 2 , Bax , N myc , c myc , pro caspase 2 and active caspase 3 was examined by immunohistochemistry in the cerebellum of six patients with sporadic CJD , three patients with olivopontocerebellar atrophy ( OPCA ) and six age matched controls . ^^^ These results indicate that enhanced Fas , Fas L , MERK , ERK , Bax and granular active caspase 3 expression is not lethal to Purkinje cells in CJD , whereas increased Bcl 2 and N myc does not preclude per se cell death or death survival in CJD and OPCA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For investigations on bcl 2 , bax , p 53 and the active form of caspase 3 , cell cultures of hLECs were pretreated with mitomycin C and processed for immunocytochemistry thereafter . ^^^ We further obtained evidence of the upregulation of the intracellular apoptotic signalling cascade , represented by bcl 2 and bax , the transcription factor p 53 and the active form of caspase 3 , after pretreatment with mitomycin C . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased expression of the anti apoptotic factor Bcl 2 or loss of pro apoptotic Bax expression attenuated dopamine induced apoptosis , suggesting the apoptosis proceeds through a mitochondrial pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Second , rolipram suppresses the expression of anti apoptotic members of the Bcl 2 family and induces the pro apoptotic protein Bax , thereby shifting the balance between pro and anti apoptotic members of the Bcl 2 family towards a pro apoptotic direction . ^^^ In conclusion , by inducing apoptosis , by enhancing apoptosis induced by fludarabine , by suppressing Bcl 2 , Bcl 10 and by inducing Bax expression , PDE 4 inhibitors may add a new therapeutic option for patients with B CLL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Domoic acid induced neuronal damage in the rat hippocampus : changes in apoptosis related genes ( bcl 2 , bax , caspase 3 ) and microglial response . ^^^ Neuronal degeneration and microglial reaction in the hippocampus were investigated , and the temporal profile of bcl 2 , bax , and caspase 3 genes in cell death or survival was assessed following the administration of DA . ^^^ Immunoreactivity of Bcl 2 and Bax was increased considerably at 16 hr and 24 hr in the neurons of the hippocampus following DA administration . ^^^ RT PCR analysis revealed that bcl 2 and bax mRNA transcripts in the hippocampus were significantly increased at 16 hr and gradually decreased at 24 hr following the administration of DA . ^^^ Although bax and bcl 2 mRNA expression is rapidly induced at early stages , in situ hybridization analysis revealed complete loss of bcl 2 , bax , and caspase 3 mRNA at 24 hr after DA administration in the region of neuronal degeneration in the hippocampus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrially dependent apoptosis involves proteins like BAX that increase mitochondrial membrane permeability and promote apoptosis and proteins like BCL 2 that decrease mitochondrial membrane permeability and reduce apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
GDNF protects against aluminum induced apoptosis in rabbits by upregulating Bcl 2 and Bcl XL and inhibiting mitochondrial Bax translocation . ^^^ This mode of administration induces cytochrome c release from mitochondria , a decrease in Bcl 2 in both mitochondria and endoplasmic reticulum , Bax translocation into mitochondria , activation of caspase 3 , and DNA fragmentation . ^^^ Coadministration of glial cell neuronal derived factor ( GDNF ) inhibits these Bcl 2 and Bax changes , upregulates Bcl XL , and abolishes the caspase 3 activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the levels of Bcl 2 and Bax , well known proteins involved in apoptosis , were evaluated . ^^^ Stable high glucose induced apoptosis in human umbilical vein endothelial cells , a phenomenon accompanied by a significant decrease of Bcl 2 and a simultaneous increase of Bax expression . ^^^ In this condition , Bcl 2 was not detectable , whereas Bax expression was significantly enhanced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
K compounds downregulated Bcl 2 , while Bax remained unchanged , reducing the ratio of Bcl 2 to Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 1beta , interferon gamma , and TNF alpha caused no major change in either islet cell survival or Bcl 2 and Bax mRNA expression after a 12 h incubation ; however , a marked increase in the amount of dead cells , with a major decrease of Bcl 2 mRNA expression , was observed after 72 h . ^^^ The presence of Th 2 , but not of Th 3 , cytokines significantly reduced beta cell death , without any major effect on Bcl 2 and Bax mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Electric microscope , TUNEL and immunohistochemical S P technique were used to detect the apoptosis , the expression of apoptosis related gene bcl 2 and bax and proliferation cell nuclear antigen ( PCNA ) of smooth muscle cells ( SMCs ) in vein graft . ^^^ CONCLUSIONS : The imbalance of proliferation and apoptosis may be related to the vessel remodeling and vein graft stenosis , and bcl 2 and bax protein may involve in the regulation of apoptosis of VSMC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tripterine could upregulate Bax , c myc expression and downregulate bcl 2 expression at protein level . ^^^ CONCLUSION : Tripterine can efficiently induce HMC 1 cell apoptosis , occurring mainly in S phase , which is correlated with upregulating Bax , c myc expression and downregulating bcl 2 expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered expression of bcl 2 mRNA and Bax in hippocampus with focal cerebral ischemia model in rats . ^^^ METHODS : Focal ischemic model in rats and dot plot hybridization were used to make an observation on the level of bcl 2 mRNA and Bax mRNA expression in the hippocampus cells after a focal ischemic injury , compared to the controls in a pseudo operation . ^^^ RESULTS : Bcl 2 mRNA was remarkably overexpressed in hippocampus area on the ischemic side after 2 hours ' ischemia and then 24 hours ' reperfusion , but the expression of Bax mRNA was increased without statistically significance . ^^^ CONCLUSION : Bcl 2 may promote the survival of neuron but Bax may induce apoptosis of the cells in hippocampus area of this model . ^^^ It is also suggested the biologic therapeutic studies , e . g . , up regulation of the expression of the bcl 2 mRNA or down regulation of expression of the Bax mRNA should be further performed , which may induce the tolerance of the brain cells to the ischemic insult . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , fumonisin B ( 1 ) had no effect on expression of bcl 2 family genes ( bax , bcl 2 , and bcl 10 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cellular apoptosis susceptibility gene expression in endometrial carcinoma : correlation with Bcl 2 , Bax , and caspase 3 expression and outcome . ^^^ Using specific antibodies for the cellular apoptosis susceptibility ( CAS ) gene , caspase 3 , Bcl 2 , and Bax , we examined the protein expression in 89 endometrial carcinomas and in 56 samples of nonneoplastic adjacent endometrium for comparison . ^^^ In nonneoplastic endometrium , CAS protein was expressed in 70 . 6 % , Bax in 64 % , caspase 3 in 52 % , and Bcl 2 in 87 % . ^^^ In neoplastic tissue , CAS was present in 93 % of the tumors , Bax in 88 % , caspase 3 in 77 % , and Bcl 2 in 51 % . ^^^ Bcl 2 : Bax ratio was > 1 in 9 cases ( 10 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Medial VSMC positive for BAX , a proapoptotic protein of the B cell CLL / lymphoma 2 ( BCL 2 ) family , showed colocalization of IGF 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To reveal whether modulation of death and survival genes is involved in leptin attenuation of follicular apoptosis , we examined the expression of the survival gene Bcl 2 and the death gene Bax in Western blots of ovarian homogenates . ^^^ Bax expression was elevated only 3 . 4 fold ( P < 0 . 001 ) , leading to an increase in the Bcl 2 / Bax ratio of 4 . 7 fold ( P < 0 . 001 ) . ^^^ Our data suggests that leptin may be involved in accelerating follicular maturation by attenuating follicular atresia and increasing the ratio of Bcl 2 / Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we report that the proapoptotic Bcl 2 family member Bax also mediates apoptosis triggered by Myc and inhibits Myc induced lymphomagenesis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Based on the antiapoptotic characteristics of BCL 2 , we further hypothesized that if a BAX component was evident then BCL 2 overexpression may be hepatoprotective . ^^^ In conclusion , these data suggest for the first time that BAX may be an early determinant of APAP mediated hepatotoxicity and that BCL 2 overexpression unexpectedly enhances APAP hepatotoxicity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of p 53 , p21 / waf1 , rb , p 16 , cyclin D 1 , p 27 , Ki 67 , cyclin A , cyclin B 1 , bcl 2 , bax and bak proteins and apoptotic index in normal thymus . ^^^ The immunohistochemical expression of p 53 , p 21 , Rb , p 16 , cyclin D 1 , Ki 67 , cyclin A , cyclin B 1 , p 27 , bcl 2 , bax , and bak proteins and the apoptotic index ( Al ) were investigated in 20 normal thymuses ( 8 adults , 3 adolescents , 5 infants and 4 newborns ) . ^^^ The expressions of Bax and bak were more widespread in both the medulla and cortex , suggesting that these proteins play a broader role than bcl 2 in the regulation of thymic apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic Bax and antiapoptotic Bcl 2 were not significantly upregulated or downregulated by expression of p 53 , p51A , or p73beta , except for p 53 expressing 70W cells , which contained a larger amount of Bax protein than LacZ expressing cells . ^^^ It is also suggested that in melanoma cells ( 1 ) cellular target protein ( s ) other than Bcl 2 and Bax might be responsible for induction of p51A mediated apoptosis and ( 2 ) caspase 3 is not always involved in the apoptosis by p 53 family members . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic lymphocytes and Bax and Bcl 2 proteins related to apoptosis were observed with in situ terminal labeling and immunocytochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Immunostaining with antibodies specific to p 53 , bcl 2 , bax , and MIB 1 was performed to evaluate expression of these proteins in formalin fixed , paraffin embedded specimens of 59 patients treated with concurrent chemoradiotherapy ( carboplatin , 100 mg / m2 , four to six times every week ; total radiation dose of 40 65 Gy over 4 6 . 5 weeks ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After neutrophils were incubated with HPWE , expression of Bcl 2 family [ antiapoptotic ( Bcl 2 , Bcl XL and Mcl 1 ) and proapoptotic ( Bax , Bak and Bcl XS ) ] was determined by RT PCR and Western blotting , respectively . ^^^ The expression of Bax and Bak was upregulated and Bcl 2 , Bcl XL and Mcl 1 downregulated in HL 60 cells during neutrophilic differentiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Gene expressions of gastrin , CCK ( B ) R , COX 1 , COX 2 and Bax and Bcl 2 was examined using RT PCR , while gastrin was measured by RIA . ^^^ CONCLUSIONS : 1 ) Colorectal carcinoma and its resection margin overexpress gastrin and receptors for gastrin ( CCK ( B ) R ) , and COX 2 ; 2 ) here , we propose that an increased plasma level of gastrin should be considered as suitable biomarker of colorectal cancer , 3 ) HP infection may contribute to colonic cancerogenesis by enhancing expression of gastrin and COX 2 , they may account for stimulation of the tumor growth , angiogenesis and reduction in apoptosis as evidenced an increased ratio of mRNA expression for anti apoptotic Bcl 2 over proapoptotic Bax proteins and 4 ) HP positive patients who develop colorectal cancer should be subjected to the HP eradication ; this is expected to reduce hypergastrinemia and to attenuate COX 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This is closely associated with [ 1 ] the down regulation of Bcl 2 and Bcl xL proteins and [ 2 ] upregulation of Bax and Bad , whose gene products are known to be involved the regulation of apoptosis in mammalian cells . ^^^ These findings suggest that modulation of Bax , Bcl xL , Bcl 2 and Bad proteins by ICI may be , in part , responsible for the anti proliferative and apoptotic effect of ICI seen clinically and in animal models . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , when AS IRE 4 was placed in 5 ' UTR of Bax gene , a pro apoptotic protein in the Bcl 2 protein family , we observed a preferential cell killing in breast cancer cells that overexpress HER 2 / neu . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transfection experiments demonstrated that Bcl 2 L 10 can block apoptosis induced by interleukin 3 withdrawal and Bax expression , by prevention of cytochrome C release , caspase 3 activation and mitochondrial membrane potential collapse . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Bcl 2 , Bax and p 21 positivity were detected immunohistochemically on paraffin embedded pre treatment biopsies from 83 patients with invasive transitional cell cancer ( TCC ) of the bladder , treated with radiotherapy . ^^^ Positivity was defined as > 10 % of tumor cells staining positive for Bcl 2 , Bax and p 21 , and > 20 % for p 53 . ^^^ RESULTS : Bcl 2 positivity was found in 63 % , Bax was positive in 52 % and p 21 in 55 % of cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Short chain fatty acids inhibit invasive human colon cancer by modulating uPA , TIMP 1 , TIMP 2 , mutant p 53 , Bcl 2 , Bax , p 21 and PCNA protein expression in an in vitro cell culture model . ^^^ To test this hypothesis , primary human invasive colonocytes were isolated from fresh surgical specimens and treated with 0 . 01 mol / L acetate , propionate or butyrate ; cell invasion , cell adhesion , F actin polymerization , urokinase plasminogen activator ( uPA ) , tissue inhibitor matrix metalloproteinase ( TIMP ) 1 , TIMP 2 and mutant p 53 , Bcl 2 , Bax , p 21 and proliferating cell nuclear antigen ( PCNA ) protein expression levels were examined . ^^^ Protein expression levels of mutant p 53 , p 21 , Bax , Bcl 2 and PCNA were significantly altered by each of the SCFA tested ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A significant decrease in Bcl 2 expression , increase in Bax expression , a loss of mitochondrial membrane potential ( delta psi m ) and a marked increase in cytochrome c expression were found only in cells treated with hyperthermia and AAPH . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 antisense in combination with Chlorambucil resulted in a more marked reduction in Bcl 2 protein expression ( p = 0 . 003 ) , enhanced Bax expression ( p < 0 . 0001 ) and increased apoptosis when compared to cells incubated with Chlorambucil alone ( p = 0 . 03 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sparse motor neurons were immunoreactive for bcl 2 , bax , bak , and CM 1 on serial sections through the spinal cord and motor cortex of individual sALS patients and controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , changes in the apoptosis regulatory proteins , daxx , bcl 2 and bax were analysed . ^^^ HDAC inhibitors induced downregulation of daxx , but no significant changes were detected in bcl 2 or bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , IPC induced upregulation of antiapoptotic gene bcl 2 and downregulation of pro apoptotic gene bax are decreased and increased , respectively , in the AG 490 treated hearts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , an increase in Bax protein and a decrease in Bcl 2 protein were detected in the mitochondrial fraction 2 h after treatment following the increases in p 53 protein level and DNA binding activity of NF kappa B . ^^^ These results led to the conclusion that Trp P 1 mainly drives the caspase 8 mediated pathway that involves Bid , accompanied by a delay in the p53 / NF kappa B mediated side pathway that involves Bax , Bcl 2 , and caspase 9 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was assessed by the in situ end labeling of double stranded DNA breaks , and by immunohistochemical characterization of cellular markers associated with apoptosis ( Fas , FasL , Bcl 2 , and Bax ) . ^^^ Expression of Bcl 2 and Bax mRNA in the corneal stroma and endothelium was separately analyzed by a semiquantitative reverse transcriptase polymerase chain reaction . ^^^ Following exposure to camptothecin , keratocytes from patients with Fuchs dystrophy responded with an increased level of Bax and a low level of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At that time , the whole kidney mRNA expression of the apoptosis inhibitory genes bcl xL and bcl 2 , as well as that of the apoptosis promotor bax , was significantly reduced . ^^^ Immunohistochemistry of kidney specimen showed suppression of bcl 2 in the distal tubule but up regulation in the proximal tubule , whereas bax protein was more strongly expressed in the distal tubule . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas low doses induced p21 / Cip1 / Waf1 and HDM 2 , high doses induced only GADD 45 and BAX increasing the BAX : BCL 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Radiation plus CE induced the up regulation of Bax and the down regulation of Bcl 2 in T cells in comparison with radiation alone . ^^^ These results suggest that CE inhibits the signal transduction pathway of apoptosis induced by radiation , regardless of the expression of Bcl 2 or Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twenty five thyroid tumors were investigated by immunohistochemistry for the presence of apoptosis , expression of p 53 , bax and bcl 2 . ^^^ In nine adenomas ( average age 40 ) the rate of p 53 positive cells was 5 . 7 % , while it was 13 . 3 % for bax and 41 . 7 % for bcl 2 in the tumor cells . ^^^ In six follicular carcinomas ( average age 56 ) p 53 positivity was obtained for 71 . 1 % , while bax and bcl 2 positivity constituted 3 . 4 % and 2 . 5 % , respectively . ^^^ In ten papillary adenocarcinomas ( average age 42 ) p 53 was positive in 39 . 6 % , but bax and bcl 2 were positive in 3 . 4 % and 3 % of the tumor cells , respectively . ^^^ Determination of p 53 , bax and bcl 2 ratio in thyroid tumors may contribute to the differentiation between follicular adenomas and carcinomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On this basis , we investigated the combined effect of Bcl 2 and Bax expression , known regulators of apoptotic processes , in the activation of apoptosis in breast cancer . ^^^ MATERIALS AND METHODS : In this study we investigated 76 T 1 ductal invasive breast cancers and 76 normal epithelium samples for Bcl 2 and Bax expression by immunohistochemistry , for apoptosis by tunel assay and for DNA content and proliferative activity by flow cytometry . ^^^ CONCLUSION : This report suggests that Bax over expression could accelerate apoptotic cell death by counteracting the ability of Bcl 2 to inhibit apoptosis . ^^^ These data also suggest that the ratio Bcl 2 / Bax and their relationship with the activation of apoptosis could be used as predictive indicators of breast cancer patients ' prognosis and response to conventional therapy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A significant decrease in BCL XL protein expression was noted with BAK , BAX , and BCL 2 unchanged , and this was corroborated at the transcriptional level with selectively decreased bcl xl mRNA production after sodium butyrate exposure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We simultaneously examined the expression of p 73 , c erbB 2 , ras , p 53 , Mdm 2 , p 27 , DCC , hMLH 1 , hMSH 2 , bcl 2 , Bax and NF kappaB , by immunocytochemistry , in both primary and metastatic melanoma cell lines derived from melanoma patients . p 73 was expressed in 7 / 8 cell lines , but stronger expressed in the metastatic cells than in the primary melanoma cells . c erbB 2 was detected in all 8 cell lines and ras in 2 / 5 metastases . p 53 was found in all the cell lines and Mdm 2 in 1 / 8 of the cell lines . ^^^ The data demonstrate that overexpression of p 73 , c erbB 2 , p 53 and bcl 2 , and loss of Mdm 2 and Bax may interact and play important roles in the development and aggressiveness of human melanoma . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment with CAPE caused rapid activation of caspase 3 after 4 h , down regulation of Bcl 2 expression after 6 h , and up regulation of Bax expression after 16 h . ^^^ These results suggest that CAPE is a potent apoptosis inducing agent ; its action is accompanied by activation of caspase 3 , down regulation of Bcl 2 , and up regulation of Bax in human leukemic HL 60 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis inducing protein , Bax , was expressed more in each of the compartments of depressed type than in those of elevated type , while there were no significant differences in expression of anti apoptotic protein , Bcl 2 , between the two types . ^^^ Moreover , the apoptosis index ( % ) of Bax positive gastric cancer was significantly higher in the basal compartment ( P = 0 . 03 ) , compared to that of Bax negative gastric cancer , while there were no significant differences in apoptosis index ( % ) in any compartment between Bcl 2 positive and Bcl 2 negative gastric cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Simultaneously , obvious G 1 arrest and apoptosis as well as the alteration of cyclin D 1 , bcl 2 and bax gene expression were observed in the infected tumor cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , the apoptotic regulatory molecules Bcl 2 , Bcl xL , and Bax , do not appear to play a significant role in the regulation of eosinophil apoptosis in the schistosome granuloma . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of 5 hydroxytryptamine 1A receptor agonist BAY 10 3702 on BCL 2 and BAX proteins level in the ipsilateral cerebral cortex of rats after transient focal ischaemia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The prognostic significance of apoptosis associated proteins BCL 2 , BAX and BCL 10 in clinical nephroblastoma . ^^^ Various apoptosis associated regulatory proteins , such as Bcl 2 , Bax and Bcl 10 , may contribute to the rate of apoptosis in neoplasia . ^^^ Generally , Bcl 2 , Bax and for Bcl 10 ( S / L ) were expressed in the blastemal and epithelial components of Wilms ' tumour . ^^^ Immunoreactive blastema cells were found in 53 % , 41 % and 38 % of tumours for Bcl 2 , Bax and for Bcl 10 ( S / L ) , respectively . ^^^ Univariate analysis showed that blastemal Bcl 2 expression and the Bcl 2 / Bax ratio were indicative for clinical progression , whereas epithelial staining was of no prognostic value . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Reduced expression of BAX is associated with poor prognosis in patients with epithelial ovarian cancer : a multifactorial analysis of TP 53 , p 21 , BAX and BCL 2 . ^^^ Using molecular and immunohistochemical analyses we examined TP 53 and its downstream genes p 21 , BAX and BCL 2 in ovarian tumour tissues and have evaluated the results in relation to clinico pathological parameters , clinical outcome and response to platinum based chemotherapy . ^^^ Those patients who simultaneously expressed BAX and BCL 2 had a longer progression free and overall survival compared to patients whose tumours did not express BCL 2 ( P = 0 . 05 and 0 . 015 respectively ) . ^^^ We conclude that BAX expression may represent a prognostic indicator for patients with ovarian cancer and that the combined evaluation of BAX and BCL 2 may provide additional prognostic significance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also demonstrated that elevated bcl 2 phosphorylation induced by vinorelbine is paralleled by decrease of a complex formation between bcl 2 and bax , cleavage of poly ( ADP ) ribose polymerase ( PARP ) protein , activation of caspase 7 , and apoptosis . ^^^ The levels of bcl 2 phosphorylation , bax , and PARP were not significantly affected by 2 ' amino 3 ' methoxyflavone ( PD 98059 ) , an ERK kinase specific inhibitor . ^^^ Thus , our data suggest that the apoptosis induced by vinorelbine in MCF 7 cells is mediated through the bcl 2 phosphorylation / bax / caspases pathways , and that activation of ERK 2 by vinorelbine does not directly lead to the drug mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As2O3 had no effect on the expression of the apoptosis related genes like Bcl 2 , Bcl xL / S , Bax , ICH 1L , p 53 , PARP , either , but downregulated PML protein expression in K 562 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We studied changes in body weight , lean body mass ( LBM ) , apoptotic index ( AI ) and expression of Bax and Bcl 2 in skeletal muscle in VX 2 carcinoma bearing rabbits . ^^^ Bax expression was detected in proportion to the AI , but no Bcl 2 expression was detected in either the experimental or control groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment with etoposide did not decrease the ratio of anti apoptotic Bcl 2 and Bcl xL proteins to pro apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the channel activity correlates with presence of proapoptotic Bax in the mitochondrial outer membrane and is absent in mitochondria from cells overexpressing antiapoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dissociation of Bax from a Bcl 2 / Bax heterodimer triggered by phosphorylation of serine 70 of Bcl 2 . ^^^ In binding assays using the phosphorylation specific antibody against pS 70 Bcl 2 , the induction of serine 70 phosphorylation 70 results in a loss of the binding ability of Bcl 2 to Bax , a pro apoptotic partner , and induces subsequent cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DESIGN AND METHODS : The aim of the present study was to explore the simultaneous expression of proteins related to both apoptosis ( APO2 . 7 , bcl 2 , bax ) and multidrug resistance ( MDR 1 , MRP , LRP ) in the different blast cell subpopulations detected at diagnosis in a group of 72 elderly patients with AML . ^^^ These results translated into a significantly ( p < 0 . 0001 ) higher bcl 2 / bax ratio for the CD34+ blast cells as compared to that of the two CD 34 blast cell subpopulations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Aloe emodin induced apoptosis of CH 27 cells involved modulation of the expression of Bcl 2 family proteins , such as BclX ( L ) , Bag 1 , and Bak , and was associated with the translocation of Bak and Bax from cytosolic to particulate fractions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bak , Bcl 2 , caspases and p 53 ) have been identified in a unicellular eukaryote , previous reports contain several implications of the apoptotic behaviour of yeasts ( i . e . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of droloxifene on apoptosis and Bax , Bcl 2 protein expression of luteal cells in pseudopregnant rats . ^^^ AIM : To study the effects of droloxifene on apoptosis and the expression of Bax and Bcl 2 protein in corpus luteum of pseudopregnant rats . ^^^ Expression of Bax and Bcl 2 protein was observed by immunohistochemistry analysis . ^^^ In pseudopregnant rats , the expression of Bax and Bcl 2 protein was found in the cytoplasm of luteal cells . ^^^ However , no obvious differences in the intensity or localization could be found during various days of the pseudopregnancy , while an increase in Bax and a decrease in Bcl 2 protein expression could be induced by droloxifene treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we report that enforced expression of either Bax or Bak via adenoviral gene delivery results in the accumulation of the proteins in the endoplasmic reticulum ( ER ) and mitochondria , resulting in early caspase independent BCL 2 sensitive release of the ER Ca ( 2+ ) pool and subsequent Ca ( 2+ ) accumulation in mitochondria . ^^^ Our results demonstrate that the effects of the `` multidomain ' ' proapoptotic BCL 2 family members Bak and Bax involve direct effects on the endoplasmic reticular Ca ( 2+ ) pool with subsequent sensitization of mitochondria to calcium mediated fluxes and cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced by droloxifene and C myc , Bax , Bcl 2 protein expression in corpus luteum of pregnant rats . ^^^ AIM : To investigate the effects of droloxifene on apoptosis of luteal cells in pregnant rats , and analyze the possible relationships between the expression of C myc , Bax , Bcl 2 protein in corpus luteum and apoptosis of luteal cells induced by droloxifene . ^^^ Ovaries were collected on d 4 or d 8 for detecting the apoptosis of luteal cells by hematoxylin eosin staining and terminal deoxyribonucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labelling ( TUNEL ) , and observing the expression of C myc , Bax , Bcl 2 protein in corpus luteum by immunohistochemistry . ^^^ In corpus luteum of droloxifene treated rats , the increased expression of C myc and Bax protein could be observed on d 4 and d 8 , respectively , whereas no obvious changes could be found in the expression of Bcl 2 protein . ^^^ An increased expression of C myc protein and Bax / Bcl 2 ratio could be induced by droloxifene , which might be associated with the mechanisms of apoptosis of luteal cells induced by droloxifene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was accompanied by upregulation of caspase 3 and 8 activity , and increased p 27 , bax , caspase 3 expression , while the levels of p21cip / waf and bcl 2 were unchanged or decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In particular , the occurrence of this process , which is less represented than we would have expected , is mediated by the balance between the well known Bcl 2 protein family members , Bad , Bax and Bcl 2 , related to the pathway PI 3 kinase / AKT 1 , which is known to deliver a survival signal . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The search for the corresponding effector genes revealed that the expression of FKBP 25 , FKBP 38 and FKBP 52 ( analysis by reverse transcription polymerase chain reaction ( RT PCR ) did not change following H ( 2 ) O ( 2 ) or FK 506 , and this was also true for the expression of apoptosis related genes caspase 3 , bax , bcl 2 and bcl xL ( analysis by Multiplex PCR ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although no difference in DNA fragmentation , Bax expression , or Bcl 2 expression was detected among wt p 53 transfectants and parental SKLMS 1 cells after Dox treatment , MDR 1 P gp expression was decreased in wt p 53 transfectants compared with parental SKLMS 1 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Twenty four mediastinal teratomas ( 18 mature and 6 immature ) were examined for apoptosis by 3 ' end labeling of DNA and stained immunohistochemically for proliferating cell nuclear antigen , Bcl 2 , Bax , p 53 protein , and alpha fetoprotein ( AFP ) expression in formalin fixed , paraffin embedded specimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In comparison , strong expression of Bax , Bak and p 21 ( waf1 / cip1 ) and suppressed expression of Bcl 2 , Bcl 10 ( L ) and COX 2 were observed in the Mn SOD antisense group and the expression pattern of these proteins was the inverse in the empty vector group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SFV mediated expression of a cloned pro apoptotic Bax gene by the vector , however , enhanced apoptosis induction both in AT 3 Bcl 2 cells and standard BHK 21 cells . ^^^ Bax expressing particles were shown to inhibit the growth of AT 3 Neo and AT 3 Bcl 2 tumours in nude mice , as did high titre EGFP expressing particles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis indicated that Tyrphostin AG 1024 induced apoptosis was associated with a downregulation of expression of phospho Akt 1 , increased expression of Bax , p 53 and p 21 , and a decreased expression of bcl 2 expression , especially when combined with irradiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of the proapoptotic Bcl 2 family member , Bax , was previously shown to induce caspase independent apoptosis , and we find that Bax is activated and translocates from the cytosol to the mitochondria in C . psittaci infected cells . ^^^ C . psittaci induced apoptosis is inhibited in host cells overexpressing Bax inhibitor 1 and is inhibited through overexpression of Bcl 2 , which blocks both caspase dependent and independent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of LMP 1 on the balance of Bcl 2 and Bax ratio may play a role in inducing susceptibility to cisplatin induced cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis was used to identify the expression of bcl 2 and bax genes . ^^^ The cell cycle of HL 60 cells was arrested in G2 / M phase , and expression of bcl 2 gene was decreased while that of bax was increased . ^^^ The inhibition might be relative to the apoptosis induced by erianin and the altered expression of bcl 2 and bax genes in HL 60 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Flow cytometric assay was used to quantify the apoptotic cells and measure the percentage of cells with positive Bcl 2 and Bax proteins . ^^^ Caspase 3 activity was determined by fluorescent spectrofluorometer and the expressive bcl 2 and bax mRNA by semiquantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ CONCLUSION : Ginsenoside Rg 1 protected PC 12 cells against apoptosis by inhibiting the activation of caspase 3 and regulating the ratio of Bcl 2 to Bax protein . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PhIP treated cells also showed higher expression of Bcl 2 and lower expression of Bax , consistent with a possible antiapoptotic action of PhIP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Adriamycin treatment modestly up regulated Apo2L / TRAIL R 2 ( DR 5 ) and had no effect on the expression of Fas associated death domain , c FLIP , Bcl 2 , Bcl ( xL ) , Bax , and IAP family members ( cIAP 1 , cIAP 2 , XIAP , and survivin ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunohistochemistry , we evaluated the expression of beta catenin , cyclooxygenase 2 ( Cox 2 ) , p 53 , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Oncol . , 16 : 333 338 , 2000 ) that genistein induces cell cycle arrest and apoptosis by up regulating p 21 ( WAF 1 ) and Bax , and down regulating cyclin B 1 and Bcl 2 in a head and neck cancer cell line . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EGCG also caused a decrease in the Bcl 2 and Bcl 10 ( L ) proteins , an increase in the Bax protein , and activation of caspase 9 , suggesting that EGCG induces apoptosis via a mitochondrial pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although GzmB directly cleaves the Bcl 2 family member BID on target cell entry , Bid deficient ( and Bax , Bak doubly deficient ) cells are susceptible to GzmB induced death , even though they fail to release cytochrome c from mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting showed that IL 15 treated Colo 320 cells exhibited downregulation of p21Waf1 and Bax , and upregulation of Bcl 2 , phospho AKT , MMP9 / MMP2 , and VEGF . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Despite the drug resistant phenotype , high Bcr Abl levels concomitantly increased the expression of p 53 , p 21 , Bax and down regulated Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The susceptibility of the developing brain to hypoxia depends on several factors : the lipid composition of the brain cell membrane ; the rate of membrane lipid peroxidation and the status of anti oxidant defenses ; the development and modulation of the NMDA receptor sites ; the intracellular Ca ( 2+ ) influx mechanisms ; expression of apoptotic and antiapoptotic genes such as Bax and Bcl 2 ; and the activation of initiator caspases and caspase 3 , the `` executioner ' ' of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Targeted gene disruptions of members of the bcl 2 and caspase gene families have demonstrated particularly significant roles for bcl 10 , bax , caspase 9 and caspase 3 in mammalian brain development . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because apoptosis seems to play a role in tumor response to radiotherapy , the current study investigates whether there is a correlation between the ratio of bcl 2 oncoprotein and bax expression in rectal adenocarcinoma and the clinical response to radiotherapy . ^^^ Immunohistochemical study , using the streptavidin biotin complex technique and monoclonal antibody to bcl 2 and polyclonal antibody to bax protein was used on paraffin sections . ^^^ Cases were considered positive if at least 5 % of tumor cells displayed cytoplasmic staining for bcl 2 or bax . ^^^ In each tumor , the bcl 2 / bax ratio was calculated dividing the percentage of bcl 2 positive cells by the percentage of bax positive cells . ^^^ The bcl 2 / bax ratio was greater in group A ( 1 . 3 + / 0 . 1 ) compared with group B ( 0 . 49 + / 0 . 1 ) , and was correlated with poor responsiveness to radiotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The authors recently showed variable subcellular immunoreactivity of the Bcl 2 and Bax proteins after fixation of cell monolayers with acetone , methanol , or paraformaldehyde ( PF ) followed by methanol ( PF / methanol ) . ^^^ In addition , Western blot analysis demonstrated loss of Bcl 2 and Bax protein during acetone or methanol fixation , whereas PF fixation before methanol permeabilization markedly reduced this loss . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The animals were sacrificed on day 8 after ulcer induction and the following parameters were analysed ; ulcer area ( planimetry ) , gastric blood flow ( GBF ) ( H 2 gas clearance method ) , gastric secretion , plasma levels of proinflammatory cytokines such as IL 1beta and TNFalpha , mucosal gene expression for cyclooxygenases ( COX 1 / 2 ) , apoptosis related proteins ( Bax , Bcl 2 ) , TNFalpha , IL 1beta and vascular endothelial growth factor ( VEGF ) . ^^^ The impairment of ulcer healing in LPS treated rats was associated with a significant decrease in GBF , increased mRNA expression for IL 1beta , TNFalpha , the rise in plasma IL 1beta and TNFalpha levels , an overexpression of COX 2 and VEGF and imbalance in the ratio between pro apoptotic Bax and antiapoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , we expanded our investigations to examine the effect of cisplatin induced ERK activation on the expression of p 53 targeted genes that have been shown to be important in the cellular response to DNA damage including Bax , Bcl 2 , Bcl x 1 , Cyclin G , Gadd 45 , p21WAF1 , and Mdm 2 . ^^^ In the ovarian carcinoma cell line A 2780 , cisplatin was shown to induce expression of p21WAF1 , Gadd 45 and Mdm 2 , but cisplatin had no effect on expression of Bax , Bcl 2 , Bcl x 1 , or Cyclin G . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The reduction in proapoptotic bax and in antiapoptotic bcl 2 mRNA levels in the whole hippocampus , suggests that local variations in these molecules could account for both neuronal viability of the CA 1 CA3 and granular cell death detected by morphological means and observed after ADX . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of different fibroblasts in media containing low level of serum and interleukin 1 beta were studied by detecting Bax and Bcl 2 protein expressions and specific DNA ladder by means of cell culture , immunohistochemistry and agrose electrophoresis . ^^^ In low level of serum , hardly occurs apoptosis of fibroblasts from keloids in correspondence with no changes of the radio of Bax / Bcl 2 proteins , and apoptosis of fibroblasts from normal skins occurs in correspondence with increasing of the radio of Bax / Bcl 2 proteins , whereas , slightly occurs apoptosis of fibroblasts from hypertrophic scars in correspondence with no change of the radio of Bax / Bcl 2 proteins . 2 . ^^^ In media containing interleukin 1 beta apoptosis of all three kinds of fibroblasts occurs , whereas , apoptosis of fibroblasts from keloids and hypertrophic scars takes place more seriously than that from normal skins in correspondence with increasing of the Bax / Bcl 2 radio of fibroblasts from keloids , decreasing of the Bax / Bcl 2 radio of ones from normal skins and no change of the Bax / Bcl 2 radio of ones from hypertrophic scars . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / Bax protein expression in heart , slow twitch and fast twitch muscles in young rats growing under chronic hypoxia conditions . ^^^ The expression of Bax and Bcl 2 proteins was determined by immunohistochemistry and Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related proteins : RAIDD , ZIP kinase , Bim / BOD , p 21 , Bax , Bcl 2 and NF kappaB in brains of patients with Down syndrome . ^^^ No significant change was detected in Bax , RAIDD ( receptor interacting protein ( RIP ) associated ICH 1 / CED 3 homologus protein with death domain ) , ZIP ( Zipper interacting protein ) kinase and NF kappaB p 65 levels in both regions , although frontal cortex levels of RAIDD , Bcl 2 and p 21 levels tended to increase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , phosphorylation of Bcl 2 induced by microtubule targeting drugs did not alter its ability to associate with Bcl 2 ( homodimerization ) , Bax , BAG 1 , or other Bcl 2 binding proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An interaction of Bax and Bcl 2 is very important to decide cell life or death , and Bcl 2 phosphorylation may control this interaction : Paclitaxel treatment induced Bcl 2 phosphorylation and typical apoptosis , whereas hyperthermia induced not Bcl 2 phosphorylation but nuclear translocation and failed to induce apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic indices ( AI ) in situ for 25 adenomas and 77 adenocarcinomas were visualized by TdT mediated dUTP nick end labeling ( TUNEL ) , Ki s 5 labeling indices ( KI ) , bcl 2 , bax , waf 1 and p 53 were immunostained with ABC method . ^^^ The well differentiated or early stage lesions with intensive bcl 2 / bax expression were significantly more likely to have low AI . p 53 accumulation and waf 1 depression were mainly related to KI , whereas bax and waf 1 overexpression led to a comparatively higher AI / KI ratio . bcl 2 and KI were found to be independent risk factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas ( TDT ) mediated deoxyuridine triphosphate ( dUTP ) biotin nick ending labeling ( TUNEL ) ; bax and bcl 2 were detected by immunohistochemical staining ( SP ) . ^^^ RESULTS : In NTP group apoptosis index ( AI ) of cytotrophoblasts ( CT ) and syncytiophoblasts ( ST ) in placenta was ( 1 . 1 + / 0 . 9 ) % and ( 41 . 8 + / 1 . 5 ) % ; bax positive rate was ( 1 . 0 + / 0 . 9 ) % and ( 28 . 9 + / 9 . 7 ) % ; bcl 2 was ( 2 . 2 + / 0 . 8 ) % and ( 22 . 9 + / 0 . 7 ) % respectively ; bax / bcl 2 was 0 . 7 1 . 7 . ^^^ In SPIH group , AI was ( 4 . 3 + / 1 . 2 ) % and ( 45 . 3 % + / 1 . 4 ) % ; bax was ( 2 . 2 + / 0 . 8 ) % and ( 42 . 5 + / 11 . 7 ) % ; bcl 2 was ( 3 . 2 + / 0 . 8 ) % and ( 23 . 3 + / 7 . 8 ) % ; bax / bcl 2 was 1 . 0 3 . 2 . ^^^ The difference between SPIH and NTP was significant respectively ( P < 0 . 01 ) ; bax / bcl 2 was balance in NTP , while the balance was broken in SPIH . ^^^ The balance and imbalance of apoptosis and bax / bcl 2 affect the structure and the function of placenta , and then may further affect the outcome of pregnancy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The expression of bcl 2 , bax , Fas and FasL were detected with immunohistochemistry method in 45 cases of NSCLC . ^^^ RESULTS : 23 ( 51 % ) cases of NSCLC were bcl 2 positive , 38 ( 84 % ) were bax positive ( P < 0 . 01 ) , and there was no significant correlation between the expression of bcl 2 and bax protein , but a positive relationship was found between the expression of Fas and FasL ( P < 0 . 01 ) . ^^^ The positive rate of bcl 2 expression decreased in advanced stages of NSCLC ( P < 0 . 05 ) , the bax expression was lower in cases with poor cell differentiation ( P < 0 . 05 ) , and the FasL expression was higher in adenocarcinoma ( P < 0 . 01 ) . ^^^ The simultaneous expression of bcl 2 and bax protein was closely correlated with TNM stages ( P < 0 . 05 ) , the differences of bcl 2 protein expression alone in NSCLC with cell differentiation were statistically significant ( P = 0 . 033 ) . ^^^ CONCLUSIONS : Both bcl 2 , bax , Fas and FasL may involve in the regulation of apoptosis and may play an important role in the occurrence and progression of NSCLC . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study the effect of estradiol ( E 2 ) and tamoxifen ( TAM ) on the apoptosis regulatory genes ( bax , bcl 2 ) and mdr 1 in MCF 7 cells . ^^^ METHODS : Immunohistochemistry , in situ hybridization were used to observe the changes in bax , bcl 2 and mdr 1 genes after treatment with E 2 and TAM . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , Bcl 2 gene expression and Bax gene expression were observed with in situ hybridization . ^^^ The Bcl 2 mRNA expression in T lymphocytes was significantly increased in different time points than in normal controls ( P < 0 . 01 ) , but Bax mRNA expression in T lymphocytes was remarkably decreased ( P < 0 . 01 ) . ^^^ The rates of Bcl 2 / Bax mRNA could not reach the state of the controls until 72 hours from the begining of the stimulation with the anti CD3+ . ^^^ CONCLUSIONS : The inhibited apoptosis might be one of the reasons for the chronic persistent inflammation in the airways in asthmatic patients and the imbalance between Bcl 2 gene expression and Bax gene expression may be one of the molecular mechanisms for the inhibited apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Data about apoptotic index , bcl 2 and bax protein expression were available in the other 91 patients . ^^^ It was also correlated with bcl 2 , bax , p 53 , p21WAF1 / CIP1 , and mdm 2 expression levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Among them are Bcl 2 family members , Noxa , PUMA , and Bax . ^^^ The Bax protein belongs to the multidomain Bcl 2 family , while Noxa and PUMA are BH 3 domain only proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The role of bcl 2 and bax genes and programmed cell death in the temporomandibular joint development ] . ^^^ OBJECTIVE : To evaluate the roles of bcl 2 , bax gene and programmed cell death ( PCD ) in the TMJ development . ^^^ METHODS : PCD , expression of both mRNA and protein of bcl 2 and expression of bax protein were observed by Tdt mediated dUIP nick end labeling ( TUNEL ) , mRNA in situ hybridization and immunohistochemical staining in TMJ of SD rats during series prenatal and postnatal developmental periods . ^^^ The expression of bcl 2 protein showed the same distribution as bcl 2mRNA , but gradually decreased in late hypertrophic zone , compared with strong expression of Bax throughout condylar cartilage . ^^^ CONCLUSIONS : PCD of condylar cartilage is regulated by bcl 2 and bax gene , the number and site of PCD are attributed to the change of bcl 2 and bax gene , and bcl 2 is an important gene in maintaining survival of chondrocytes during the proliferation , differentiation and maturation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the effects of cadmium on the bcl 2 family of proteins bcl 2 , bax , bad , and bcl xS / L in cadmium induced cytotoxicity . ^^^ Western blot analyses revealed that cadmium markedly increased endogenous bcl 2 protein ( to 3 4 times the level in wild type cells ) earlier than metallothionein induction , but that the metal did not enhance the induction of bax , bad , or bcl xS proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , SDF 1 significantly increased the viability of CD 34 ( + ) T cell precursors modulating the expression of BCL 2 and BAX genes , and stimulated the proliferation of CD 34 ( + ) thymic precursor cells , particularly in synergy with interleukin 7 ( IL 7 ) , but not with other cytokines , such as stem cell factor or flt 3 ligand . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show that BAX and BAK , two closely related death promoting members of the BCL 2 family , are expressed in Sertoli cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells , the nasopharyngeal carcinoma cell line CNE 2 was used to identify the effect of heparin on apoptosis associated with the expression of c myc , bax , bcl 2 proteins by use of Hoechst 33258 staining , terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) , agarose gel electrophoresis , and flow cytometry , as well as Western blot analysis . ^^^ Western blot analysis showed that levels of bcl 2 , bax and c myc were significantly overexpressed by treatment with the increase of heparin concentrations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treated JM 1 cells , on the other hand , showed an increase in the expression of Bcl 2 protein in both forms of cell death , but failed to show Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression in human endometriotic and adenomyotic tissues . ^^^ OBJECTIVE : To determine the Bcl 2 and Bax expressions in endometriotic and adenomyotic tissues . ^^^ In addition , to evaluate the Bcl 2 / Bax status during the menstrual cycle in these tissues . ^^^ Bcl 2 and Bax expressions were investigated on 56 formalin fixed and paraffin embedded tissue by immunohistochemical staining and electron microscopy . ^^^ We have found a stronger inverse correlation between the expression of Bcl 2 and Bax in endometriosis than in adenomyosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Light microscope , transmission electron microscope , DNA electrophoresis , flow cytometry and RT PCR assay were used to observe the changes of human leukemia cells in morphology and biochemistry , and Bcl 2 / Bax expression after exposing to FCNNAV . ^^^ RT PCR detection showed the Bcl 2 gene expression of HL 60 cells was down regulated by FCNNAV , whereas the Bax expression was unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We hypothesized that estrogen might promote the development of lupus by altering apoptosis of bone marrow cells , perhaps through regulation of the apoptotic proteins Bax and Bcl 2 . ^^^ We compared the effects of estrogen ( E 2 ) and thrombopoietin ( TPO ) on the expression of Bax or Bcl 2 in bone marrow cells isolated from female non lupus ( NZW or NZB parental strains ) or lupus prone ( NZB and NZW cross ; NZB / W ) mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , while expression levels of apoptosis inhibitors , Bcl 2 and Bcl XL were increased following UC treatment , that of an apoptosis promoter , Bax , was decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of both anti apoptotic ( such as Bcl 2 and Bcl 10 ( L ) ) and proapoptotic ( such as Bax ) proteins is markedly elevated in the liver of human ALD and chronically ethanol fed IL 6 ( + / + ) mice . ^^^ On the contrary , induction of Bcl 2 and Bcl 10 ( L ) is not observed in the liver of chronically ethanol fed IL 6 ( / ) mice , whereas expression of Bax protein remains elevated . ^^^ Injection of IL 6 markedly induces expression of Bcl 2 and Bcl 10 ( L ) but not Bax in the liver . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : Apoptosis index ( AI ) , Bcl 2 , and Bax have shown prognostic significance in head and neck squamous cell carcinoma ( HNSCCa ) . ^^^ MATERIALS AND METHODS : Formalin fixed paraffin embedded tissue samples of 10 normal oral mucosa , 15 reactive / dysplastic lesions , and 17 HNSCCa lesions studied previously were subjected to the terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP labeling ( TUNEL ) assay as well as immunohistochemical staining against Bcl 2 , Bax , and p 53 . ^^^ RESULTS : Compared with normal oral mucosa , the AI , Bcl 2 , Bax , Bcl 2 / Bax intensity and frequency ratios , and mutant p 53 intensity significantly changed in reactive / dysplastic and HNSCCa lesions ( P < . 001 for all ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Bcl 2 , an antiapoptotic protein , showed a strong tendency to decrease with age , whereas Bax , a proapoptotic protein , remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As biomarkers , expression of Bcl 2 , Bax , Bcl 10 , and the accumulation of P 53 protein were also studied immunohistochemically . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ratios of the apoptosis regulating proteins Bcl 2 to Bax and Bcl 10 ( L ) to Bax were higher in cells overexpressing wild type , but not NLS mutated , PTHrP compared with control cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We conclude that a central role exists for the AHR in transducing the actions of PAHs in fetal ovarian germ cells , and that the proapoptotic Bcl 2 family member , Bax , is a required mediator of PAH induced oocyte loss in female fetuses exposed to PAHs in utero . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The regulation of apoptosis by Bcl 2 , bcl 10 ( L ) , Bcl 2alpha and Bax in chronic liver disease ] . ^^^ OBJECTIVE : To analyse the expression of Fas and Fas ligand ( FasL ) in patients of chronic hepatitis , liver cirrhosis and hepatocellular carcinoma and to study the regulation of apotosis by Bax and Bcl 2 subfamilies . ^^^ The expression level of Bax subfamily in the chronic hepatitis group was significantly higher than that of Bcl 2 subfamily . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression and distribution of Fas , FasL , Bcl 2 and Bax proteins in the specimens were investigated with immunohistochemical methods . ^^^ Positive rates of Fas , FasL , Bcl 2 and Bax in thyroid cells of NTG ( 35 % approximately 47 % ) were significantly lower than those in thyroid cells of HT ( 88 % approximately 94 % , P < 0 . 01 ) . ^^^ The intensity of positive immunostaining for Fas , FasL , Bcl 2 and Bax in thyroid cells of HT was significantly higher than that of the controls ( P < 0 . 01 ) . ^^^ Apoptosis and strongly positive immunostained thyroid cells with HT for Fas , FasL and Bax were mainly distributed in follicles adjacent to lymphocytic infiltrates , whereas strongly positive stained thyroid cells for Bcl 2 were mainly located in the area remote from the infiltrating lymphocytes . ^^^ Staining of infiltrating lymphocytes for Fas , FasL , Bcl 2 and Bax was weak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of receptor blockade and injury on the expression of viability related genes were also assessed by comparing relative bcl 2 , bax , and p 53 messenger RNA levels using in situ hybridization analysis . ^^^ Spironolactone and RU 486 decreased basal bcl 2 messenger RNA levels in CA 1 and dentate gyrus but did not affect basal bax or p 53 levels . ^^^ Injury decreased bcl 2 messenger RNA levels in dentate gyrus but did not affect bax or p 53 levels in vehicle pretreated animals . ^^^ RU 486 modulation of bcl 2 , bax , or p 53 messenger RNA expression does not predict neuronal viability at this time point , suggesting that RU 486 mediated preservation of CA 1 neurons does not involve transcriptional regulation of these cell death related genes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nitration of Bax and Bcl 2 proteins during hypoxia in cerebral cortex of newborn piglets and the effect of nitric oxide synthase inhibition . ^^^ Previous studies have shown that cerebral tissue hypoxia results in increased expression of Bax protein , thereby altering the ratio of Bax to Bcl 2 or formation of Bax / Bcl 2 heterodimer . ^^^ The present study tests the hypothesis that tissue hypoxia will result in nitration of Bax and Bcl 2 proteins in the neuronal nuclei of newborn piglets . ^^^ We speculate that , during hypoxia , nitration of Bax and Bcl 2 proteins may regulate heterodimer formation and activation of programmed cell death mechanisms . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : To detect cell specific apoptosis factors , Fas and Fas ligand , and the common intracellular apoptosis modulators , interleukin 1 beta converting enzyme ( ICE ) like protease ( caspase 1 ) , Bcl 2 , Bcl xL and Bax in lens epithelial cells ( LEC ) of human cataracts . ^^^ METHODS : Reverse transcriptase polymerase chain reaction ( RT PCR ) was used to detect Fas , Fas ligand , caspase 1 , Bcl 2 , Bcl xL and Bax , after cDNA was synthesized from the total RNA isolated from human cataractous LEC obtained by capsulotomy during cataract surgery . ^^^ RESULTS : Fas , caspase 1 , Bcl 2 , Bcl xL and Bax mRNA were detected by RT PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results suggest that GH acts as survival factor during in vitro culture and reduces apoptosis by altering the bax to bcl 2 ratio during early embryogenesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of upstream events of apoptosis pathway showed that the expression of Bax was induced and bcl 2 was inhibited by Cpd 5 treatment . ^^^ PD 98059 , a mitogen activated protein kinase kinase inhibitor , and glutathione , an anti thiol oxidant , not only blocked Cpd 5 induced ERK phosphorylation , but also antagonized the activation of CPP 32 , the altered Bcl 2 / Bax expression , and DNA fragmentation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Taken together , KR 31378 strongly inhibited cell death in HUVECs in association with antiapoptotic effects , which were accompanied by up regulation of Bcl 2 protein expression and down regulation of Bax protein and suppression of cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In conclusion , cilostazol and its analogs exert a strong protection against apoptotic cell death by scavenging hydroxyl radicals and intracellular ROS with reduction in TNF alpha formation and by increasing Bcl 2 protein expression and decreasing Bax protein and cytochrome c release . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of Bcl 2 delays Bax conformational change , cytochrome c release , and apoptosis induced by EpoB . ^^^ Taken together , these findings demonstrate that EpoB induces apoptosis through a Bcl 2 suppressible pathway that controls a conformational change of the proapoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No changes in the expression of Bax , Bid and Bik ( pro apoptotic proteins ) , or Bcl 2 ( an anti apoptotic protein ) were detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thirty eight serrated adenomas were examined by the nick end DNA labeling method and immunostained for CD 95 ( Fas ) , bcl 2 , bax , and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect was correlated with a marked decrease of Bcl 2 mRNA expression ( without any major change of Bax mRNA ) and a marked increase of inducible nitric oxide synthase mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As apoptosis is regulated by several proteins , we investigated the expression of p 53 , Bax and Bcl 2 in maxillary SCC before treatment and after preoperative chemoradiotherapy using an immunohistochemical approach . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bax neutralizing antibodies , cDNA expression of a p53R273H dominant negative mutant , and caspase inhibitors prevent Abeta 1 42 mediated human neuronal cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We evaluated the expression of apoptosis regulating proteins ( p 53 , Bcl 2 , Bax , Bak and Mcl 1 ) in paraffin embedded tissues of 33 patients with diffuse large B cell non Hodgkin ' s lymphoma , and assessed the relationship of these proteins to clinical outcome and response to chemotherapy . ^^^ Bcl 2 , Bax , Bak and Mcl 1 proteins , though highly expressed in almost all cases were not associated with prognosis or response to treatment . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and BAX mRNA concentration profile in fibrillary astrocytoma . ^^^ A high level of the BCL 2 protein and the lack of apoptosis promoting protein BAX are beginning to be treated as markers of cellular resistance to anti neoplastic drugs . ^^^ The molecular analysis conducted in order to determine the sensitivity of the tumour to radio or chemotherapy included the determination of the number of mRNA BCL 2 alpha and beta molecules and of BAX in 1 microg total RNA obtained from microscope slides . ^^^ A higher expression of BAX than of BCL 2 alpha is a prognosis for a positive result of chemo or radiotherapy . ^^^ A trace number of mRNA BCL 2 beta molecules and a smaller number of mRNA BCL 2 alpha molecules than mRNA BAX is a good prognosis for therapy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , in HCa 1 , the application of a priming dose increased radiation induced Bcl XL and Bcl XS , but not Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect was not apparently mediated through downregulation of the PK hBcl 2 transgene or via delocalization of the Bcl 2 protein , and a direct interaction of HBx with Bcl 2 , Bcl 10 ( L ) or Bax could not be evidenced in yeast two hybrid assays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of Caki 1 cells with 5 FU enhanced the expression of p 53 and bax , but had no effect on the expression of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of renal cell carcinoma and apoptotic related genes bcl 2 and bax ] . ^^^ METHOD : We detected the apoptotic status and the expression of apoptotic related gene bcl 2 and bax protein in 48 cases of RCC with TdT mediated dUTP biotin nick end labeling ( TUNEL ) and immunohistochemistry methods . ^^^ RESULT : 22 cases showed low expression of bcl 2 protein with an average apoptotic index ( AI ) of 1 . 35 and 26 cases had high expression of bcl 2 with an AI of 1 . 04 ( P < 0 . 05 ) . 34 cases showed low expression of bax protein with average AI of 1 . 03 and 14 cases had high expression of bax protein with an average AI of 1 . 63 ( P < 0 . 01 ) . ^^^ The stronger bcl 2 expression , the weaker bax expression and the less number of apoptotic cells . ^^^ CONCLUSION : both bcl 2 and bax take part in the occurrence and progression of RCC . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cellular apoptosis , proliferation and bcl 2 Bax expression in colorectal carcinoma and their association with tumor prognosis ] . ^^^ OBJECTIVE : To investigate the relationship between cellular apoptosis , proliferation and bcl 2 / bax expression in the tissue of colorectal carcinoma and biological behavior of the tumor . ^^^ METHOD : The apoptotic cells index ( AI ) in situ were identified by the terminal deoxynucleotidyl transfer mediated dUTP biotin nick end labeling ( TUNEL ) and immunohistologic staining for Ki 67 proliferation index ( KI ) , bcl 2 / bax protein expression was performed on archival material from 77 adenocarcinomas . ^^^ There was a positive relationship between the AI and KI in each specimen . bcl 2 and bax was expressed as 54 . 5 % , 74 % in carcinoma and highly differentiated carcinoma . ^^^ CONCLUSION : The data indicate that abnormally exchanged AI / KI , bcl 2 / bax expression appears to be an event associated with tumor progression and apoptosis might also reflect the proliferative activity of human colorectal carcinoma . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The reduction in infarct size was accompanied by decreases in myocardial lipid peroxidation and in proapoptotic Bax and proinflammatory interleukin 1beta protein abundance , concomitant with an increase in antiapoptotic Bcl 2 protein level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This signaling pathway can be modulated by different pro and anti apoptotic proteins such as Bax and Bcl 2 , which are localized at the mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression patterns of cell cycle and / or apoptosis associated p 27 , p 21 ( cip / waf ) , bax , bcl 2 , cyclin A and ( pro ) caspase 3 were studied using quantitative Western blotting . ^^^ TSA increased activation of caspase 3 and expression of cyclin A , p2l ( cip / waf ) , bax and ( pro ) caspase 3 , while bcl 2 was downregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : In our earlier series we showed that ciprofloxacin inhibits bladder tumor cell growth with concomitant S / G2M cell cycle arrest and reported an increased Bax to Bcl 2 ratio in cells undergoing cell death . ^^^ Our findings also showed Bcl 2 dependent subcellular redistribution of Bax to the mitochondrial membrane in ciprofloxacin treated bladder tumor cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The regulatory proteins Bcl 2 , Bax , and Bak constitute a diffusion driven molecular switch with inherent damping of apoptosis induction , thereby controlling the apoptosis reaction cascade under noisy , external apoptosis inducing conditions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein kinase A RIalpha antisense inhibition of PC3M prostate cancer cell growth : Bcl 2 hyperphosphorylation , Bax up regulation , and Bad hypophosphorylation . ^^^ In addition , Bcl 2 hyperphosphorylation ; increase in the proapoptotic proteins Bax , Bak , and Bad ; and Bad hypophosphorylation occurred in the antisense treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : BAX , a proapoptotic member of the BCL 2 family of proteins , has been detected in Hodgkin and Reed Sternberg ( HRS ) cells of Hodgkin ' s disease ( HD ) , but its clinical significance is unknown . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dynamics of expression of apoptosis regulatory proteins Bid , Bcl 2 , Bcl 10 , Bax and Bak during development of murine nervous system . ^^^ We have used immunohistochemistry and immunoblotting to examine the expression of Bid and four other Bcl 2 family proteins ( Bcl 2 , Bcl 10 , Bax and Bak ) in the developing and adult murine central nervous system ( CNS ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of our study was to determine whether mRNA levels of Mdm 2 , Bcl 2 , Bcl 10 ( L ) , Bad , and Bax are independent prognostic parameters for outcome . ^^^ In patients who subsequently failed to enter complete remission ( CR ) , high levels of Bad , Bax and Bax high / Bad high were associated with an increased relative risk ( RR ) to die from tumor ( RR = 5 . 0 for Bad , 3 . 4 for Bax and 6 . 14 for Bax high / Bad high ) . ( 2 ) Following ICT , high expression of Bax ( P= 0 . 005 ) and high Bcl 2 / Bax ratios ( P = 0 . 004 ) were independent predictors of unfavorable outcome , regardless of response to ICT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As shown by flow cytometry and confirmed by Western blotting , apoptosis was associated with a decrease in the ratios of Bcl 2 / Bax and Bcl ( XL ) / Bax , due to an increase in the level of Bax , but no change in that of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of pro and anti apoptotic proteins p 53 , p 21 , MDM 2 , BCL 2 , BCL 10 ( L ) , BCL 10 ( S ) , and BAX , and caspase 3 activity was determined in circulating blasts collected from the peripheral blood of children with leukemia prior to , and at serial time points following chemotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a proapoptotic member of the Bcl 2 family , localizes largely in the cytoplasm but redistributes to mitochondria in response to apoptotic stimuli , where it induces cytochrome c release . ^^^ Expression of Akt did not alter the levels of Bax , Bcl 2 , Bcl 10 ( L ) , or phosphorylated JNK under the conditions used , suggesting that there were alternative mechanisms for Akt in the suppression of Bax translocation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of this study was to evaluate the expression of anti and pro apoptotic proteins , ( Bcl 2 , Bax ) , and to correlate this with the appearance of some nuclear receptors and cell cycle related proteins in androgen sensitive and androgen insensitive prostate cancer cell lines , LNCaP and DU 145 , after the treatment by androgen antagonist bicalutamide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IFN gamma or TRAIL treatment alone did not change expression of other pro or antiapoptotic proteins such as DR 4 , DR 5 , FADD , Bax , IAP 1 , XIAP , Bcl 2 , and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Preconditioning was found to target specifically the mitochondrial control of apoptosis in EC with increased protein levels of Bcl 2 , decreased protein levels of Bax , and decreased cytosolic release of cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunohistochemistry and Western blotting , Bcl 2 and Bax expression was determined in a panel of multidrug resistant RCC lines featuring Pgp and / or at MDR . ^^^ In the drug resistant sublines , some decreased Bax and strongly increased Bcl 2 expression was seen by immunohistochemistry indicating prevention of apoptosis as a distinct feature of MDR in RCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related genes such as Bax , Bcl 10 ( L ) , Bcl 2 or caspase 8 were reduced by p27Kip1 transduction compared with that of beta actin , whereas p27Kip1 transduction did not affect the expression level of Fas or the Fas ligand . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : To clarify whether nitrotyrosine expression is associated with apoptosis and / or angiogenic factors in ESCC , we have analyzed the relationship between nitrotyrosine presence and the apoptosis related proteins , Bcl 2 and Bax , or CD 34 , the marker of vascular endothelial cells , by an immunohistochemical approach . ^^^ The correlation between nitrotyrosine presence and Bcl 2 , Bax expression or apoptotic index ( AI ) was not significant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , Bcl 2 and Bax expression was investigated by Northern blot and immunocytochemistry while the p 53 mutation status was analyzed by DNA sequencing . ^^^ No correlation became evident between responsiveness to topotecan and the expression levels of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the role of apoptosis related proteins ( Myc , Bax , Bcl 2 , and Bcl 10 ( L ) ) in muscular damage in obstructed rat ureters . ^^^ The numbers of apoptotic cells in the smooth muscle layer correlated significantly with the expressions of Myc and Bax ( r = 0 . 7360 and 0 . 7432 , respectively ; both P < 0 . 005 ) , and with the expression index of Bax / Bcl 2 and Bax / Bcl 10 ( L ) ( r = 0 . 8909 and 0 . 8592 , respectively ; both P < 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in the apoptosis related proteins , bcl 2 , bcl xL , and bax were analyzed by Western blotting . ^^^ Moreover , treatment with AG 490 inhibited IL 6 induced proliferation of ACHN and 769P cells and induced apoptosis with the down regulation of bcl 2 and the up regulation of bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Infection of metastatic melanoma cells with Ad . mda 7 results in an increase in cells in the G ( 2 ) / M phase of the cell cycle and changes in the ratio of pro apoptotic ( BAX , BAK ) to anti apoptotic ( BCL 2 , BCL XL ) proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 : Bax ratio was also not significantly changed in calnexin deficient cells regardless of whether the ER stress was induced with thapsigargin or not . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 family proteins ( Bcl 2 , Bcl 10 , Bax , Bak and Bim ) in human lymphocytes . ^^^ We investigated the expression of Bcl 2 , Bcl 10 , Bax , Bak and Bim in human lymphocytes using flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
They had been investigated by the in situ end labeling and immunohistochemical staining to detect the apoptotic cells and the expression of Fas , FasL , bcl 2 and Bax . ^^^ There was significant positive correlation between the number of the apoptotic cells and that of the cells expressing Fas , FasL and Bax , respectively , and there was significant negative correlation between the number of the apoptotic cells and that of the cells expressing bcl 2 . ^^^ In SS lacrimal glands , the expression of Fas , FasL and Bax may promote apoptosis , and the expression of bcl 2 may inhibit apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We focused on the apoptotic signaling molecules cytochrome c , Bcl 2 and Bax . ^^^ Moreover , there is a loss of Bcl 2 from mitochondria while , in contrast , Bax accumulates in this organelle following t BHP treatment . ^^^ This event is stimulated by Bcl 2 disappearance from mitochondria and Bax recruitment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time points protein expression levels of p 53 , p21 / WAF1 , Bax , and Bcl 2 and the activity of caspase 3 were measured . ^^^ The protein expression levels of p 53 , p21 / WAF1 , Bax , and Bcl 2 varied in time , but were not related to the apoptotic behavior of the cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 10 , Bcl 2 , Bax , and Bak in endarterectomy and atherectomy specimens . ^^^ The aim of this study was to determine the expression of Bcl 2 , Bcl 10 , Bax , and Bak in relation to apoptosis in advanced atherosclerotic lesions . ^^^ In conclusion , increased Bax and Bak coupled with lack / paucity of Bcl 2 and Bcl xL are associated with SMC apoptosis in advanced lesions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alteration of the Bcl 2 : Bax ratio in the placenta as pregnancy proceeds . ^^^ In particular , we evaluated the expression of Bcl 2 and Bax and their ratio during the first and third trimester . ^^^ These data indicate that Bcl 2 and Bax are spatio temporally regulated during placental development and that the different expression of the above mentioned genes is at least in part responsible for the delicate balance between cell proliferation and programmed cell death in the human placenta during pregnancy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of excessive iodine on apoptosis and expression of bcl 2 , bax genes in the cells of brain and thyroid gland in guinea pigs ] . ^^^ OBJECTIVE : To investigate the effects of excessive iodine intake on apoptosis and the expression of bcl 2 , bax genes in the cells of brain and thyroid gland . ^^^ Apoptosis rate and expression of bcl 2 , bax gene in the cells of cerebral cortex , hippocampus and thyroid gland in guinea pigs were quantitatively determined by flow cytometry and immunofluorescent techniques . ^^^ The level of expression of bcl 2 gene was notably decreased ( P < 0 . 05 ) , but that of bax was notably increased ( P < 0 . 01 ) , in the cerebral cortex , hippocampus and thyroid gland in the group with excessive iodine than in the control one . ^^^ The ratio of bcl 2 to bax in the cerebral cortex , hippocampus and thyroid gland of the group with excessive iodine was decreased significantly ( P < 0 . 01 ) than that of the control one . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Murine embryonic fibroblasts from bax ( / ) bak ( / ) mice exposed to nitric oxide during hypoxia did not die , indicating that pro apoptotic Bcl 2 family members are required for NO induced apoptosis during hypoxia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Samples from the regions corresponding to the penumbra showed markedly reduced Bcl 2 protein level and , in contrast , high levels of Bax protein and cytochrome c release . ^^^ Cilostazol decreased Bax protein and cytochrome c release and increased the levels of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the results of immunofluorescence and RT PCR assays showed that the apoptosis regulator , Bcl 2 was downregulated after treatment with IL 13 toxin , while Bax was upregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the apoptosis of retinal capillary cells in early diabetic rats and observe the expression of apoptosis related genes ( bcl 2 , Bax ) in retinas of early diabetic rats . ^^^ In ABC staining method , two genes ' ( bcl 2 , Bax ) proteins were expressed in retinal vessels . ^^^ Moreover , pigment epithelial cells in DM 3 showed Bcl 2 positive reaction that again extended further to inner rod segments and ganglion cells in DM 6 , while in DM 6 , Bax was expressed in ganglion cells . ^^^ CONCLUSIONS : The nature of pericyte dropout in early diabetic rats is apoptosis that also exists in endothelial cells ; the expression of Bax and bcl 2 in retinas of early diabetic rats is enhancing . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The growth rate , ultrastructure , density of apoptotic cells , rate of bcl 2 and bax oncoprotein expression and its positive cell density in transplanted tumor of nude mice from colorectal carcinoma were investigated by employing transmission microscopy , in situ end labeling technique and immunohistochemical staining . ^^^ Many tumor cells at varied apoptotic stages could be observed in transplantation tumor tissue in the bifidobacterium prevention group and their density of apoptotic cells , rate of bcl 2 and bax oncoprotein expression and its positive cell density were significantly higher in the group with bifidobacterium prevention , as compared with the control group ( P < 0 . 01 ) . ^^^ But , expression rate of bcl 2 oncoprotein turned contrary to that of bax oncoprotein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was evaluated by : deoxyribonucleic acid ( DNA ) fragmentation electrophoresis , DNA content , annexin 5 binding , apoptosis related markers ( Fas , B cell lymphona leukaemia 2 ( Bcl 2 ) , Bax , and CD 25 ) , and by electron microscopy . ^^^ In asthma , this effect was associated with a significant decrease of Bcl 2 expression , and with an increase of Bax / Bcl 2 ratio . ^^^ Moreover , in IL 2 stimulated PBTs from both asthmatics and normal subjects , FP was able to induce apoptosis and to reduce Bcl 2 , Fas and CD 25 expression , whereas negligible effects were detected on Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax , Bcl 2 , p 53 , and p 21 was examined by Western blot . ^^^ The ratio of Bax / Bcl 2 was elevated , and p 53 and p 21 mRNA were over expression . ^^^ CONCLUSION : Heparin and ADM appear to interact in a synergistic manner , which may be related to the over expression of p 53 and p 21 mRNA and the elevated ratio of Bax / Bcl 2 mRNA . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : In order to make clear how As ( 2 ) O ( 3 ) targets the cells of esophageal carcinoma , the morphology of mitochondria and expression of bcl 2 / bax were selected to study the SHEEC 1 cell line during early apoptosis . ^^^ The expression of bcl 2 and bax in floating cells were determined by immunohistochemical procedures . ^^^ In all groups , down regulated expression of bcl 2 and over expression of bax were always found in damaged cells . ^^^ CONCLUSION : In the SHEEC 1 cell line morphological changes of mitochondria are the early events in apoptosis induced by As ( 2 ) O ( 3 ) and apoptosis is closely related to over expression of bax and down regulating expression of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : p 53 , bcl 2 , Fas , bax expression were detected immunohistochemically in 61 cases of colorectal adenoma with either mild or severe dysplasia and carcinoma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression rate of bax was the opposite to that of bcl 2 . ^^^ The mechanism of cell death may be correlated with apoptosis , and bcl 2 and bax gene may play an important role in regulating cerulenin induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In 102 cases of STSs , apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method and expression of Bcl 2 , Bax , MIB 1 and p 53 protein was examined immunohistochemically . ^^^ The mean values of positive cell staining for Bcl 2 , Bax , MIB 1 and p 53 protein were 32 . 1 , 40 . 8 , 17 . 0 and 20 . 3 % , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bcl 10 , Mcl 1 , Bax and Bak in human uterine leiomyomas and myometrium during the menstrual cycle and after menopause . ^^^ To investigate the expression of Bcl 2 , Bcl 10 , Mcl 1 , Bax and Bak proteins in human uterine leiomyomas and homologous myometrium during the menstrual cycle and after menopause . ^^^ The expression of Bcl 2 , Bcl 10 , Mcl 1 , Bax and Bak in leiomyomas ( n=24 ) and myometrial samples ( n=22 ) from women with leiomyomas was measured by immunohistochemistry and Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Bcl 2 , Bax , Bcl 10 , Fas ( CD 95 ) and tumor necrosis factor ( TNF ) receptor expression was determined by flow cytometry in control and mitomycin C treated Tenon fibroblasts . ^^^ RESULTS : Tenon fibroblasts constitutively express Bcl 2 , Bax , and Bcl 10 in culture . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Bcl 2 protein expression was significantly up regulated in the clones while no significant changes in Bax , Bcl ( xL ) , P glycoprotein and Hsp 70 protein expression and no consistent changes in p 53 expression were detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Estrogen receptor ( ER ) , HER2 / neu , metablastin , beta tubulin 3 and 4 , microtubule associated protein 4 ( MAP 4 ) , bcl 2 , bax , and cyclooxygenase 2 ( COX 2 ) gene expression were measured using real time quantitative polymerase chain reaction ( PCR ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor cell resistance to death receptor induced apoptosis through mutational inactivation of the proapoptotic Bcl 2 homolog Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of anti apoptotic Bcl 2 and pro apoptotic Bax was analyzed by semiquantitative Western blot analysis and activity of caspases 3 , 8 and 9 by substrate cleavage assays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of oncogenic transcription factors , c Myc , B Myc , Max and Mad , and apoptotic genes , namely Bcl 2 , Bax and Bad , was increased after FB 1 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this work , we demonstrate that treatment of VSMCs with simvastatin and atorvastatin inhibited Bcl 2 expression in a time and dose dependent manner , while Bax expression was not modified . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of protein bcl 2 , bax have no change in response to squamocin treatment in HL 60 cells , whereas stress activated protein kinase ( SAPK / JNK ) was activated after treatment with squamocin in HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax was also detected in dying T cells , which can explain the absence of the protective effect of Bcl 2 . because Bax inhibits Bcl 2 death repressor activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
VCA induced apoptosis by down regulation of Bcl 2 and by up regulation of Bax functioning upstream of caspase 3 in both cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Archival tissue from six chronic pancreatitis cases was compared with six normal controls using TUNEL and immunohistochemistry for CD 3 , CD 20 , CD 68 , MIB 1 , Bcl 2 , Bax , Fas , Fas ligand , retinoblastoma protein ( Rb ) , and tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) and 2 ( TIMP 2 ) . ^^^ Acinar Bcl 2 expression exceeded islet expression in chronic pancreatitis and controls while Bax was strongly expressed by a subset of islet cells and weakly by centroacinar cells . ^^^ Acinar Bcl 2 and islet Bax expression indicates complex AI control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Large cell lung carcinoma and adenomatous squamous lung carcinoma demonstrated the highest proliferation and spontaneous apoptosis of tumor cells with accumulation in these cells of p 53 , bcl 2 and bax comparing to non small cell lung carcinoma ( NSLC ) with adenomatous differentiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The predictive value of bcl 2 , bax , bcl xL , bag 1 , fas , and fasL for chemotherapy response in advanced breast cancer . ^^^ In 126 patients , histological blocks of primary tumors were available for immunohistochemical analysis of bax , bcl 2 , bcl xL , bag 1 , fas and fasL . ^^^ RESULTS : Of the investigated factors , bag 1 correlated positively with bax , bcl 2 , and fasL , and fasL correlated positively with fas and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of thymidylate synthase ( TS ) , p 53 , retinoblastoma protein ( Rb ) , Fas receptor , Fas ligand , bcl 2 , mcl 1 , bax , and bcl 10 was measured using immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In both systems , expression of the anti apoptotic protein Bcl 2 was markedly increased , whereas expression of the pro apoptotic protein BAX was reduced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of mRNA levels of p 53 regulated genes involved in cell cycle control ( p 21 , gadd 45 , and cyclin G ) or apoptosis ( bax and bcl 2 ) showed that during chronic genotoxic stress , bone marrow cells from p53+ / + mice expressed significantly higher levels of a majority of these genes compared to p53+ / bone marrow cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ribonuclease protection assays compared the effects of acetaminophen and E 2 on expression of selected genes ( c myc , c fos , cyclin D 1 , bcl 2 , bax , gadd 45 , mcl 1 , p 53 , p 21 ( CIP1 / WAF1 ) , and bcl xL ) in E 2 responsive breast cancer ( MCF 7 ) and endometrial adenocarcinoma ( Ishikawa ) cells as well as in E 2 nonresponsive ( MDA MB 231 ) breast cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bid is the only known Bcl 2 family member that can function as an agonist of proapoptotic Bcl 2 related proteins such as Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since no consistent differences in Bcl 2 , Bcl 10 ( L ) or Bax expression were seen in the STS and radiation resistant neuroblastomas , it suggests that a unique mitochondrial signaling factor ( s ) is responsible for the defect in cytochrome c release in this sub group of tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical studies of regulator proteins p 53 , Bcl 2 , Bax , Ki 67 , and CD 95L showed that apoptosis suppression , allowing unlimited proliferation of tumor cells , plays an important role in the carcinogenesis of uveal melanoma . ^^^ Prognostic significance of p 53 , Bcl 2 , Bax , and CD 95L was demonstrated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The two parent proteins increase the acetyl DEVD al sensitive caspase 3 like activity in both HEK 293 cells and Telencephalon specific murine neurons , modulate Bax and bcl 2 expressions , and enhance cytochrome C translocation into the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BPA increased the expression of Bax and concomitantly decreased the expression of Bcl 2 at both protein and mRNA levels of granulosa cells . ^^^ Up regulation of Bax and down regulation of Bcl 2 were suggested to be involved in this apoptotic effect . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The other ovary was used for immunohistochemical analysis of selected members of the B cell lymphoma / leukemia 2 family of protooncogenes ( Bax , Bcl 2 , Bcl 10 ) , proliferating cell nuclear antigen ( PCNA ) , and cyclin dependent kinase 2 ( Cdk 2 ) . ^^^ Further , the results indicate that Smad 3 may regulate the expression of Bax and Bcl 2 , but not Bcl 10 , Cdk 2 , and PCNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The findings of samples from penumbral zone , which showed markedly reduced Bcl 2 protein level and increased Bax protein and cytochrome c release , were wholly reversed by treatment with KR 31378 . ^^^ In conclusion , postischemic treatment with KR 31378 provided significant levels of cortical neuroprotection in association with inhibition of apoptotic cell death through the up regulation of Bcl 2 expression , and the down regulation of Bax protein and cytochrome c release . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In patients with untreated CD there was a 2 . 3 fold higher expression of Bak mRNA ( p = 0 . 026 ) , without significant differences in the expression of related genes bax or bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show that in lymphatic cells Par 4 overexpression decreases the level of Bcl 2 , whereas Bax , the proapoptotic counterpart of Bcl 2 , retains unaltered levels . ^^^ Our study is the first to identify Par 4 as a proapoptotic protein in lymphatic cells , outlining a model of action evaluating the role of Bcl 2 / Bax , as well as demonstrating the impact of Par 4 expression on PARP cleavage , disruption of mitochondrial membrane potential , caspase activation , and interactions with inhibitors of apoptosis proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Stable transfection of a kinase dead , dominant negative PKCzeta mutant in U 937 cells decreases Bcl 2 expression while increasing the expression of Bax and several procaspases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Baxpsi tumors exhibit a slow proliferation in Swiss nude mice and this feature can be circumvented by the co expression of the Bcl 2 transgene , the functional antagonist of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To determine whether hyperoxia induced TUNEL represented apoptosis , we labeled sections with anti Bax ( proapoptotic ) and anti Bcl 2 ( antiapoptotic ) . ^^^ The large effects of hyperoxia on TUNEL were not accompanied by substantial effects on Bax , Bcl 2 , or M 30 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of an anti apoptotic NF kappaB target , Bcl 2 , were increased fourfold whereas proapoptotic proteins Bax and Bak decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , ML 1 cells grown on the clinostat showed elevated amounts of the apoptosis associated Fas protein , of p 53 , and of bax but showed reduced quantities of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Diazoxide increased the levels of Bcl 2 and inhibited the association of Bax with mitochondria in neurons exposed to an apoptotic insult , suggesting that activation of mitochondrial ATP sensitive potassium channels may stabilize mitochondrial function by differentially modulating proapoptotic and antiapoptotic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resistance of colon cancer cells to long term 5 fluorouracil exposure is correlated to the relative level of Bcl 2 and Bcl 10 ( L ) in addition to Bax and p 53 status . ^^^ In addition , we found that high levels of anti apoptotic Bcl 2 and Bcl 10 ( L ) proteins combined with a low level of Bax were correlated to high 5 FU resistance of wild type p 53 cell lines . ^^^ In conclusion , the relative levels of Bcl 2 , Bcl 10 ( L ) and Bax may altogether contribute to determine the resistance of a majority of colon tumor cells to long term 5 FU treatment , whatever their p 53 status . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA expression levels of the bcl 2 genes family was changed as follows : bax increased up to 40 % in CCI vs the sham rats , while bcl 2 did not change ; bcl xS massively decreased ( by 70 % and 100 % ) , while bcl xL increased ( by 40 % ) in CCI rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting was used to evaluate the following in proteins : poly ( ADP ribose ) polymerase ( PARP ) , caspases 8 and 3 , Bcl 2 , heat shock protein ( HSP ) 70 , Bax , Bak and Stat 1 ( a protein known to be inducible by IFN gamma ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of bcl 2 , bcl 10 , bax , p 53 and p 21 were determined by Western blotting , and celi cycle analysis was determined by flow cytometry . ^^^ Neither IL 1 nor ceramide induced apoptosis in EEC , but they increased bcl 2 levels and decreased bax and p 21 levels without affecting bcl 10 and p 53 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of nitric oxide ( NO ) donor sodium nitroprusside ( SNP ) on the proliferation and apoptosis and on Bcl 2 , Bax and p 53 proteins of pulmonary fibroblasts were investigated by using MTT cleavage assay , agarose gel electrophoresis and flow cytometric analysis . ^^^ In the meantime , the level of Bcl 2 decreased and that of Bax increased , while the p 53 remained unchanged . ^^^ Down regulation of Bcl 2 and up regulation of Bax are implicated in the molecular mechanisms of this action . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression were examined by Western blot and bcl 2 mRNA expression was examined by Northern blot . ^^^ However , PGA 1 did not alter Bax protein expression resulting in an increase in the ratio of Bcl 2 to Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Western and Northern blot analyses , as well as coimmunoprecipitation assays , revealed that in both MCF 7 and MDA MB 231 cells , DIM treatment decreased total transcript and protein levels of the apoptosis inhibitory protein Bcl 2 , and the amount of Bcl 2 bound to the pro apoptotic protein Bax . ^^^ DIM treatment also caused an increase in Bax protein levels , but did not affect the level of Bax that was bound to Bcl 2 . ^^^ These results demonstrate that DIM can induce apoptosis in breast cancer cells independent of estrogen receptor status by a process that is mediated by the modulated expression of the Bax / Bcl 2 family of apoptotic regulatory factors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Coronary reperfusion following ischemia : different expression of bcl 2 and bax proteins , and cardiomyocyte apoptosis . ^^^ To this end , we studied the expression of the pro and anti apoptotic factors , bax and bcl 2 , respectively , in reperfused ischemic hearts and in hearts injected with bFGF or saline . ^^^ Sections from the left ventricle were stained with anti bcl 2 antibody and anti bax antibody using the TUNEL method which detects apoptosis . ^^^ Ischemia / reperfusion increases the overall expression of both bcl 2 and bax proteins , but bcl 2 is lost from the reperfused area as indicated by TUNEL staining . ^^^ Accordingly , the ratio of bcl 2 to bax was reduced in the reperfused area , indicating a pro apoptotic trend . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This decrease in cellular Bcl 2 staining was not accompanied by a concomitant loss of staining for the pro cell death Bax protein , suggesting that post traumatic neuronal death in the cortex may be dependent on altered cellular ratios of Bcl 2 : Bax . ^^^ No changes in either cellular Bcl 2 or Bax expression were observed in the thalamus or white matter at any time post injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IFN gamma promoted TRAIL mediated activation of caspase 8 , Bcl 2 interacting domain death agonist ( Bid ) degradation , Bcl 2 associated 10 protein ( Bax ) translocation to mitochondria , cytochrome c release to the cytosol and activation of caspase 9 in these cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Lymphocytes treated by extracorporeal photopheresis demonstrate a drop in the Bcl 2 / Bax ratio : a possible mechanism involved in extracorporeal photopheresis induced apoptosis . ^^^ Previous work has demonstrated a close association between the genes p 53 , Bcl 2 and Bax and apoptosis induced by UV irradiation . ^^^ METHOD : Lymphocytes were isolated immediately before treatment and immediately prior to re infusion and tested for intracellular levels of p 53 , Bcl 2 and Bax proteins . ^^^ RESULTS : No increase in p 53 expression was observed at re infusion ; however , the mean fluorescent intensity ratio of the apoptotic inhibitor protein Bcl 2 to the apoptosis inducing protein Bax dropped significantly . ^^^ CONCLUSION : The early apoptosis observed in ECP treated lymphocytes at re infusion might be attributed to dysregulation in the expression of the apoptotic genes Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 , bax and p 53 protein in the basal cell carcinoma ] . ^^^ The expression of bcl 2 , bax and p 53 protein was studied in the basal cell carcinoma with 32 patients by immunohistochemical method . ^^^ The results showed that bcl 2 protein was positive in 26 patients ( 81 . 2 % ) , bax protein was positive in 9 patients ( 28 . 1 % ) , and p 53 protein was positive in 13 patients ( 40 . 6 % ) ; there is a negative correlation between the expression of bcl 2 and bax protein ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Indeed , DC activation effectively inhibited DC apoptosis , which was predominantly accompanied by the upregulation of Bcl 10 ( L ) and to a lesser extent Bcl 2 , while Bax and FLICE inhibitory protein ( FLIP ) remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro . ^^^ The objectives of this study are : ( 1 ) to investigate whether differences in the quality of immature oocytes and embryo fragmentation are associated with apoptosis ; and ( 2 ) to study the pattern of Bcl 2 and Bax expression in oocytes and embryos to help elucidate their potential roles in the regulation of apoptosis during development . ^^^ The expression of Bcl 2 and Bax in oocytes and embryos of different qualities and stages was determined using western blotting . ^^^ This implies that the ratio of Bcl 2 to Bax may be used to gauge the tendency of oocytes and embryos towards either survival or apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We characterized the immunohistochemical expression of p 53 , Mdm 2 , Bcl 2 , and Bax in 51 intracranial ependymomas . ^^^ The expression of Mdm 2 , Bcl 2 , and Bax were not related to survival . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , ER , PR , bcl 2 and bcl 2 associated 10 protein ( Bax ) expression levels were determined in meningioma cytosols . ^^^ The expression levels of the anti and pro apoptotic proteins , bcl 2 and Bax , respectively , were determined by immunoblotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis regulating proteins p 53 , Bcl 2 , and Bax in primary resected esophageal squamous cell carcinoma . ^^^ We used antibodies specific for the human p 53 , Bcl 2 and Bax proteins to examine the expression of these apoptosis regulating proteins in 40 archival specimens of patients with primary resected esophageal squamous cell carcinoma . ^^^ The overall expression of p 53 , Bcl 2 , and Bax was 73 % , 18 % , and 100 % , respectively . ^^^ No significant correlations were found between the expression of p 53 , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Results of immunohistochemistry showed a significantly positive relationship between the expression of p 53 and Bax and Bcl 2 proteins , but not Waf 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of lead acetate on the apoptosis and the expression of bcl 2 and bax genes in rat brain cells ] . ^^^ OBJECTIVES : To explore the effect of lead acetate on the apoptosis of rat brain neural cells and the relationship between the apoptosis and the bcl 2 as well as bax gene expression . ^^^ The rates of apoptosis and the expression of bcl 2 ( Bcl 2 ) and bax ( Bax ) in neural cells from cerebral cortex , hippocampus and carebellum were measured respectively by flow cytometry ( FCM ) . ^^^ The expression of bcl 2 was significantly decreased , whereas bax was significantly increased , in neural cells from cerebral cortex , hippocampus and cerebellum in every lead acetate treatment group ( FI ) compared with the control group , and there was a significant dose response relationship ( r = 0 . 886 , 0 . 787 and 0 . 832 respectively for bcl 2 , r = 0 . 971 , 0 . 988 and 0 . 991 respectively for bax ) . ^^^ The value of Bcl 2 / Bax in every treatment group decreased significantly compared with control , and there was a nice dose response relationship ( r = 0 . 863 , 0 . 829 and 0 . 999 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression and significance of apoptosis related protein p 53 , Bcl 2 , and Bax during the development of oral squamous cell carcinoma ( SCC ) . ^^^ RESULTS : In normal mucosa , the positive rate of p 53 , Bcl 2 and Bax were 0 % , 20 % and 60 % . ^^^ In dysplasia epithelia , the positive rate of p 53 is increased ( P < 0 . 05 ) , the positive rate of Bcl 2 and Bax remained no significant change ( P > 0 . 05 ) , but the positive intensity in severe dysplasia was higher than in mild group . ^^^ In SCC , the positive rate of Bcl 2 increased significantly ( compared with dysplasia , P < 0 . 05 ) , while the expression of Bax was decreased with the increase of SCC histological grade . ^^^ Further analysis showed the correlation was evident in p 53 and Bax in dysplasia , and in p 53 and Bcl 2 in SCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The grafted veins were harvested at 3 , 1 , 2 and 4 week respectively after the operation . intimal hyperplasia ( IH ) , smooth muscle cell ( SMC ) , proliferation , p 53 , bcl 2 and bax were observed pathologically and immunohistochemically . ^^^ There was no significant difference in expression of P ( 53 ) between the two groups , and there was a significant increase in bax / bcl 2 in the radiation group at 2 weeks ( t = 9 . 783 , P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we examined the lobe specific and age dependent expression of Bcl 2 and Bax proteins . ^^^ In all three lobes , Bcl 2 was detected in epithelial cells , but not in stromal cells , whereas Bax protein was localized in both cell types . ^^^ In the dorsal and lateral lobes , neither Bcl 2 nor Bax expression was significantly altered after castration . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of ischemia reperfusion ( IR ) and ischemic preconditioning ( IP ) on hemodynamics , epicardial electrography , myocardial infarct size , cardiomyocytic apoptosis and gene proteins involving apoptosis ( Fas , Bcl 2 and Bax ) were observed in aneasthetized rabbit myocardium . ^^^ Bcl 2 / Bax ratio of IR group was lower than that in non ischemic myocardium ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , rifampicin down regulated the expression of Bax and CD95L and up regulated the expression of Bcl 2 , Bcl xL , and Flice inhibitory protein L ( FLIPL ) ; however , rifampicin had no effect on CD 95 or XIAP expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bax , bcl 2 , caspase 3 , and AT ( 1 ) and AT ( 2 ) receptors was examined by Western blot analysis . ^^^ The bax to bcl 2 ratio , expression of the active form of caspase 3 ( 17 kDa ) , and activity of caspase 3 in the hearts of the ANG 2 group increased more than twofold above controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Peptides corresponding to the BH 3 domains of Bax ( BaxBH 3 ) or Bcl 2 ( Bcl2BH3 ) are potent inducers of apoptosis when fused to the Atennapedia plasma membrane translocation domain ( Ant ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ceramide induced apoptosis is accompanied by accumulation of p 53 followed by an increase of Bax and decrease of Bcl 2 levels . ^^^ Inhibition of p 53 expression with p 53 antisense oligonucleotides inhibits apoptosis and prevents the increase in Bax and decrease in Bcl 2 . ^^^ These results suggest that p 53 regulates the ratio Bcl 2 / Bax and the expression / activation of caspases during ceramide induced apoptosis in SKN SH cells . ^^^ Caspase inhibition did not alter the expression of p 53 , Bcl 2 and Bax . ^^^ Thus ceramide induced reduction in the Bcl 2 / Bax ratio , increase in caspase activity , and apoptosis is dependent upon increases in cellular p 53 levels which play a critical role in the regulation of apoptotic cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bad , Bax , Bcl 10 ( S ) ) were reduced 2 4 fold in the resistant cell line , whereas the anti apoptotic proteins Bcl 2 and Bcl 10 ( L ) were expressed at similar levels in both cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cellular commitment to apoptosis is partly regulated by the Bcl 2 family proteins , which includes the death antagonists Bcl 2 and Bcl 10 ( L ) , and death agonists Bax and Bad . ^^^ Cellular expression of Bcl 2 , Bcl 10 ( L ) , Bax or Bad in MS patients was independent of the expression of other apoptotic regulatory molecules , such as Fas receptor protein or FLIP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the chemopreventive potential of ITCs while reproducing the circumstances of dietary contact with sulforaphane , we studied proliferation , apoptosis induction and p 53 , bcl 2 and bax protein expression in Jurkat T leukemia cells by sulforaphane , the ITC generated in situ in a quantitative manner by Myr starting from glucoraphanin ( GRA ) . ^^^ Cell cycle progression , apoptosis and expression of p 53 , bax and bcl 2 proteins were analyzed by flow cytometry . ^^^ Moreover , it markedly increased p 53 and bax protein expression , and slightly affected bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When compared to the primary tumor , more anti apoptotic bcl 2 and less pro apoptotic bax were expressed in the liver and lymph node metastatic cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IVIG decreased the intracellular expression of anti apoptotic proteins of the Bcl 2 family ( A 1 and Bcl XL ) while IVIG increased the intracellular expression of pro apoptotic proteins ( Bax and Bcl XS ) in the TNF alpha stimulated HUVECs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We measured mRNA and protein expression of heme oxygenase ( HO ) 1 , Bcl 2 and Bax at different time points after Gln administration . ^^^ The ratios of Bcl 2 / Bax mRNA expression after Gln administration peaked at 3 h and reduced thereafter until 24 h . ^^^ Bcl 2 immunoreactive protein was enhanced in Gln group , whereas Bax was faintly detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of rhIL 6 on Bcl 2 and Bax expression and apoptosis after anoxia reoxygenation in cultured rat hippocampal neurons ] . ^^^ The purpose of the present study was to determine the effects of recombinant human interleukin 6 ( rhIL 6 ) on the Bcl 2 and Bax expression and apoptosis after anoxia reoxygenation in cultured rat hippocampal neurons . ^^^ The expression of Bcl 2 and Bax was revealed immunocytochemically using the antiserum against Bcl 2 and Bax . ^^^ The results showed that in cultured hippocampal neurons the Bcl 2 expression decreased while Bax expression and the percentage of apoptotic neurons increased after anoxia reoxygenation compared with those before anoxia . ^^^ In comparison with the control , after anoxia reoxygenation the Bcl 2 expression in hippocampal neurons was higher than that in rhIL 6 group ; however the Bax expression and the percentage of the apoptosis were decreased in rhIL 6 group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Particular attention is paid to the role of cytochrome c release from mitochondria and alterations in the pro and anti apoptotic proteins , Bax and Bcl 2 , respectively . ^^^ Our results demonstrate that a tissue specific adaptation of the Bcl 2 / Bax ratio occurs with age and may directly influence the release of cytochrome c . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR studies revealed no major change of iNOS and Bax mRNA expression and a marked decrease of Bcl 2 mRNA expression in the islets cultured with FFA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RANKL had no effect on H / RS cell survival in culture , and it did not modulate the expression of bcl 2 , bcl xL , bax , or inhibitors of apoptosis proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Total RNA was extracted from cells at different treatment doses and subjected to reverse transcriptase polymerase chain reaction for the semiquantitative analysis of COX 2 , Bcl 2 , and bax expression . ^^^ Treatment of both cell lines with dmPGE ( 2 ) resulted in dose dependently higher expression of COX 2 , Bcl 2 , and bax mRNA compared with untreated cells . ^^^ These effects were associated with overexpression of COX 2 and an increase in the ratio of Bcl 2 : bax mRNA . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The expression of Bcl 2 , Bax , caspase 1 ( ICE ) and caspase 3 ( CPP 32 ) were examined in bilateral testicular specimens from 26 infertile men with varicocele and six normal testicular specimens , using the avidin biotin peroxidase complex method . ^^^ RESULTS : Bax , ICE , and CPP 32 were expressed in germ cells , while Bcl 2 was not . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate this hypothesis further , we designed a novel approach to correlate immunoexpression of Ki 67 , bcl 2 and bax in the spindle cell and in the solid glandular component of biphasic synovial sarcomas . ^^^ METHODS AND RESULTS : Immunohistochemistry for vimentin ( 10 cases ) , bcl 2 ( 10 cases ) , Ki 67 ( 10 cases ) , cytokeratin ( 10 cases ) , and bax ( eight cases ) , and double immunostaining for vimentin / Ki67 ( 10 cases ) , vimentin / bcl 2 ( nine cases ) , cytokeratin / Ki67 ( 10 cases ) , and cytokeratin / bcl 2 ( 10 cases ) assays were performed in 10 cases of primary biphasic synovial sarcoma . ^^^ On conventional immunohistochemistry , the solid / glandular component revealed more expression of Ki 67 , bax and cytokeratin than the spindle cell component ( P=0 . 0004 , P=0 . 082 , and P < 0 . 0001 , respectively ) ; on the other hand , the latter showed higher expression of bcl 2 and vimentin than the former ( P=0 . 0281 and P=0 . 059 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SN 50 induced apoptosis in MM cell lines and patient cells ; down regulated expression of Bcl 2 , A 1 , 10 chromosome linked inhibitor of apoptosis protein ( XIAP ) , cellular inhibitor of apoptosis protein 1 ( cIAP 1 ) , cIAP 2 , and survivin ; up regulated Bax ; increased mitochondrial cytochrome c release into the cytoplasm ; and activated caspase 9 and caspase 3 , but not caspase 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related parameters ( Bax , Bcl 2 , CD 95 , and CD 95 induced apoptosis ) did not account for differential susceptibility to drug induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of proteins and cytokines participating in the apoptotic process ( caspase 1 , caspase 3 , Fas [ CD 95 ] , Fas ligand [ Fas L ] , perforin , granzyme A , Bax , WAF 1 , Bcl 2 , interleukin 2 [ IL 2 ] , IL 18 , and granulocyte macrophage colony stimulating factor ) in tissue in the acute and convalescent stages of dysentery was quantified at the single cell level by in situ immunostaining . ^^^ Significantly reduced cell death during convalescence was associated with a significant up regulation of Bcl 2 , Bax , and WAF 1 expression in the rectum compared to that in the acute phase of infection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate the effect of neoadjuvant chemotherapy on gastric cancer , we examined the correlation between induction of apoptosis and expression of p 53 , Bcl 2 , and Bax . ^^^ There was no significant correlation between AI and expression of Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical studies of Bcl 2 , Bax , cytochrome c , Apaf 1 , activated caspase 3 and human inhibitor of apoptosis protein XIAP , and immunoblotting of Apaf 1 and XIAP were also carried out . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We searched for immunoreactivity of glial cells for the apoptotic related markers death receptor ( DR ) 3 , Bax and Bak , the anti apoptotic marker Bcl 2 and the cell cycle marker Ki 67 . ^^^ The pro apoptotic markers Bax , Bak and DR 3 were all modestly increased in glial cell cytoplasm in CPM , while there was no change in expression of Bcl 2 , which was only sparsely detected in glial cells both in controls and cases of CPM . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : We tested the hypothesis that apoptotic trophoblasts from pregnancies associated with fetal growth restriction caused by preeclampsia or cigarette use exhibit enhanced expression of the proapoptotic proteins p 53 and Bax and diminished expression of the antiapoptotic protein Bcl 2 . ^^^ The expression of Bax , Bcl 2 , Bak , and Bcl 10 ( L ) was analyzed by immunoblotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Multidrug resistance ( MDR 1 ) , multidrug resistance associated protein ( MRP ) , glutathione S transferase pi ( GST pi ) , Topoisomerase 2 alpha ( TOPO IIalpha ) , thymidylate synthase ( TS ) , thymidine kinase ( TK ) , Ki 67 , BAX and BCL 2 genes were analysed in a series of 30 renal cell carcinomas ( RCC ) and 16 biopsies from adjacent normal kidney ( ANK ) tissue using reverse transcription PCR ( rt PCR ) . ^^^ BAX and BCL 2 expression in RCC was significantly higher than that of ANK ( 0 . 51 + / 0 . 08 versus 0 . 18 + / 0 . 12 , p = 0 . 0001 ; 0 . 73 + / 0 . 16 versus 0 . 5 + / 0 . 22 , p = 0 . 01 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , Bimgamma mRNA , similar to the BimEL protein , is up regulated in the majority of the prostate cancer cell lines studied , whereas several other proapoptotic Bcl 2 proteins , including Bax , Bak , and Bad , are down regulated in prostate cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Homozygous deficiency for bax , a proapoptotic member of the Bcl 2 family , saved the same proportion of neurons as GDNF , suggesting that GDNF alone was sufficient to maintain all `` rescuable ' ' MNs for at least 1 week . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the Bcl 2 family is critically involved in the regulation of apoptosis , we investigated the protein expression of Bcl 2 , Bcl 10 ( L ) , and Bax in peripheral blood mononuclear cells ( PBMC ) of 23 MS patients and 29 control subjects . ^^^ In contrast to Bcl 10 ( L ) , no differences were found in the protein expression of Bcl 2 and Bax between patients and controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Evaluation of mRNA and protein levels of the apoptotic regulatory proteins Bcl 2 and Bax showed a prominent decrease in the amounts of Bcl 2 mRNA and protein in aged eggs , whereas Bax mRNA levels did not appear to be changed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High mutation levels of cell cycle control genes such as p 53 , p 16 , p 21 , SMAD 4 , and cyclin D 1 are found , and there is abnormal expression of apoptotic genes , such as bcl 2 , bcl XL , and bax . ^^^ However , the inactivation and loss of expression of p 16 , p 53 , and p 21 , and the expression of several apoptotic genes , such as bax and bcl 2 , have not been found to be of any prognostic significance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The function of the second domain is still unknown . vMIA does not share any significant amino acid sequence homology with Bcl 2 , and , unlike Bcl 2 or Bcl 10 ( L ) , it does not bind BAX or VDAC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pro apoptotic BCL 2 family member , BAX , is also activated faster in cells expressing normal CFTR than in those with mutant CFTR under these conditions , and artificial glutathione depletion increases the extent of BAX activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and expression of Bcl 2 , Bcl XL , and Bax in renal cell carcinomas . ^^^ The present study analyses the inter relationship between the expression of representatives of the anti apoptotic ( Bcl 2 , Bcl XL ) or pro apoptotic ( Bax ) Bcl 2 proteins , incidence of apoptosis , and mitosis in a selected small group of 22 graded RCCs that had paired normal renal tissue , or non neoplastic tissue in the renal biopsy specimen . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased cardiomyocyte apoptosis following ischemia and reperfusion in diet induced hypercholesterolemia : relation to Bcl 2 and Bax proteins and caspase 3 activity . ^^^ In this study , we examined whether hypercholesterolemia increases the extent of myocardial reperfusion injury by aggravating cardiomyocyte apoptosis and the effects of hypercholesterolemia on the expression of Bcl 2 and Bax proteins and the activation of caspase 3 . ^^^ Western blot analysis showed that , in the nonischemic myocardium , hypercholesterolemic rabbits exhibited an approximately 50 % increase in the expression of Bcl 2 ( P < 0 . 05 ) , but not Bax , than control rabbits . ^^^ However , compared with controls , hypercholesterolemic rabbits exhibited a more pronounced decrease in the expression of Bcl 2 ( 42 + / 4 vs . 26 + / 2 % , P < 0 . 01 ) and a similar extent of increase in the expression of Bax in the ischemic myocardium . ^^^ This study demonstrates that although hypercholesterolemia is associated with an increased myocardial Bcl 2 / Bax ratio at baseline , it still significantly exacerbates cardiac reperfusion injury , not only by increasing the infarct size but also by increasing the extent of cardiomyocyte apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By using a ribonuclease protection assay , the ratios of both pro and antiapoptotic mRNA ( bcl 10 , bcl 2 , bax , caspase 2 , and caspase 3 ) to the housekeeping glyceraldehyde phosphate dehydrogenase ( GAPDH ) gene were determined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Involvement of nuclear factor kappa B , Bax and Bcl 2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells . ^^^ These effects were found to correlate with a shift in Bax / Bcl 2 ratio more towards apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : We examined the expression of Bcl 2 , Bcl xl , Bax , caspase 3 , and inducible nitric oxide synthase in infiltrating mononuclear cells of colorectal cancer tissues and also in colorectal cancer tissues . ^^^ The increased apoptosis might have resulted from an imbalance of antiapoptotic and proapoptotic molecules , as reflected by reduction of Bcl 2 level and elevation of Bax level . ^^^ The elevated caspase 3 levels found in this study could be a downstream effector of the Bcl 2 and Bax apoptotic pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 and bax proteins in spinal cord was detected with immunohistochemistry . ^^^ CONCLUSIONS : NGF can protect the injured nerve tissues through stimulating the expression of bcl 2 protein , inhibiting the expression of bax protein and inhibiting the neuronal apoptosis after SCI . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the absence of IL 7 , pro T cells undergo programmed cell death , which has previously been associated with a decline in Bcl 2 and translocation of Bax from cytosol to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the potential role of the BCL 2 gene family ( BAX , BCL 2 , MCL 1 , and BCL XL ) in ovarian cancer development and progression , mRNA expression levels of these genes were measured using semi quantitative PCR in epithelial ovarian tumor tissues and normal ovaries . ^^^ These results suggest that increased MCL 1 expression may play an important role in replacing the functions of increased BAX and decreased BCL 2 in ovarian carcinoma cells , thereby promoting cell survival , and resulting in a poor prognosis for patients with ovarian cancer . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate the biochemical processes involved in the pathogenesis of B CLL and MCL , we analyzed the expression level of a set of genes that play central roles in apoptotic or cell proliferation pathways and of candidate genes from frequently altered genomic regions , namely ATM , BAX , BCL 2 , CCND 1 , CCND 3 , CDK 2 , CDK 4 , CDKN1A , CDKN1B , E2F1 , ETV 5 , MYC , RB 1 , SELL , TFDP 2 , TNFSF 10 , and TP 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein levels of vascular endothelial growth factor ( VEGF ) , endothelial nitric oxide synthase ( eNOS ) , Bax , and Bcl 2 were determined by Western blot analysis in hindlimbs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To analyze the underlying mechanisms of drug resistance , we compared endogenous Bax and Bcl 2 expression to ex vivo response to eight drugs , and to survival in 39 B CLL patients . ^^^ In contrast to the ex vivo data , neither Bax or Bcl 2 expression nor doxorubicin sensitivity were associated with increased survival whereas sensitivity to fludarabine correlated with better overall survival ( P = 0 . 031 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using a quantitative solid phase radioimmunoassay ( RIA ) , we measured the cellular protein levels of Bcl 2 , cyclin D 1 , PCNA , ATM , Fas , Bax , retinoic acid receptor alpha ( RARalpha ) , retinoic acid receptor beta ( RXRbeta ) , Flt 1 , VEGF , and cellular beta 2 microglobulin in 230 samples of CLL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased expression of the pro apoptotic Bax together with decreased Bcl 2 suggests Bax mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes of bcl 2 and bax mRNA expressions in the ethanol treated mouse brain . ^^^ To characterize the biochemical mechanism of cell death induced by ethanol intoxication , we examined expression of mRNAs of bcl 2 and bax genes in the brain , which are related to apoptosis , by using the reverse transcription polymerase chain reaction method ( RT PCR ) . ^^^ We found that bcl 2 or bax mRNA expressions in the brain were changed after short term ethanol exposure . ^^^ These results suggest that bcl 2 or bax may have functional significance about ethanol intoxication . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Mtb induced apoptosis was associated with a speedy and transient increase in expression of Bax protein , a proapoptotic member of the Bcl 2 family , and a more prominent reduction in expression of the antiapoptotic protein Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis , cell proliferation , and Bcl 2 and Bax expression were examined at the epithelial and stromal levels in the eutopic endometrium from patients with endometriosis before and after 30 days of daily exposure to COCs and from controls . ^^^ MAIN OUTCOME MEASURE ( S ) : Apoptotic cells were detected by using the dUTP nick end labeling assay ; Ki 67 , Bcl 2 , and Bax expressions were assessed by using immunohistochemical techniques . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Rasagiline increased the levels of bcl 2 and bcl 10 ( l ) mRNA at 100 10 nM and 100 10 pM , but not the level of pro apoptotic bax mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , gene expression analysis revealed that EGCG prevented both the 6 OHDA induced expression of several mRNAs , such as Bax , Bad , and Mdm 2 , and the decrease in Bcl 2 , Bcl w , and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The prognostic importance of p 53 , bcl 2 , and bax in early stage epithelial ovarian carcinoma treated with adjuvant chemotherapy . ^^^ In a series of 109 patients with epithelial carcinomas in FIGO stages IA IIC , a number of clinicopathologic prognostic factors ( age , FIGO stage , histopathologic type , and tumor grade ) were studied in relation to the biologic factors p 53 , bcl 2 , and bax , which are important regulators of apoptosis . ^^^ The combined bcl 2 bax expression was related to histopathologic subtype ( P = 0 . 045 ) and tumor grade ( P = 0 . 022 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , we investigated the ability of synthetic peptides derived from proteins of the Bcl 2 family , namely , the NH 2 terminal region of Bcl 2 ( Bcl2 _ syn ) , a central domain of Bax ( Bax _ syn ) , and a central domain of Bak ( Bak _ syn ) to interfere with the apoptotic process in LLC PK 1 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This substance promoted cell death via a mitochondrial signaling pathway , that is , by activation of Bid and Bax , loss of the mitochondrial transmembrane potential , release of cytochrome c , and activation of effector caspases , even under conditions of Bcl 2 overexpression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Malignant pleural mesothelioma lines and tumors rarely express the antiapoptotic Bcl 2 protein but routinely express the antiapoptotic protein Bcl xl and the proapoptotic proteins Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis of the protein extracts from these cells showed down regulation of Bcl 2 , altering the ratio of Bax : Bcl 2 favoring apoptosis . ^^^ Further studies in this area are warranted as the roles of p21WAF1 , Bax / Bcl 2 and NF kappaB may be important molecular events in mediating the antiproliferative and apoptosis inducing effect of etoposide in combination with ciprofloxacin in HRPC cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our data demonstrated that Gyp induced apoptotic cell death was accompanied by up regulation of Bax , Bak and Bcl 10 ( L ) , and down regulation of Bcl 2 and Bad , while it had no effect on the level of Bag 1 protein . ^^^ Taken together , these results suggest that treatment of human hepatoma cells with Gyp induced apoptosis through the up regulation of Bax and Bak , and down regulation of Bcl 2 , release of mitochondrial cytochrome c and activation of caspase cascade . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After cyanide ( 100 500 microm ) treatment for 24 h , two pro apoptotic Bcl 2 proteins , Bcl 10 ( S ) and Bax were up regulated as shown by western blot and RT PCR analysis . ^^^ The expression levels of two antiapoptotic Bcl 2 proteins , Bcl 2 and Bcl 10 ( L ) , remained unchanged after cyanide treatment , whereas the mRNA levels of Bcl 10 ( S ) and Bax began to increase within 2 h and their protein levels increased 6 h after treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also immunohistochemically assessed for expression of BCL 2 ( anti apoptosis ) , BAX ( pro apoptosis ) , and P 53 and correlated the results with apoptosis and proliferation . ^^^ We detected a high AI ( median 8 . 1 % ) in 11 cases of SPTCL , and 12 cases had low BCL 2 and high BAX expression . ^^^ BCL 2 expression inversely correlated with AI ( P < . 001 ) and BAX ( P < . 001 ) . ^^^ Our findings demonstrate that SPTCLs have a high AI that may be explained by differential expression of BCL 2 and BAX in the neoplastic cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting for Bcl 2 family members revealed no significant changes in the expression levels of Bcl 2 , Bcl 10 ( L ) , Bax or Bak following gene or ' chemogene ' therapy with E2F 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also sought to determine whether such an antiapoptotic effect was related to Bcl 2 / Bax protein and caspase 3 activity . ^^^ Kidney function , histomorphologic findings , in situ terminal deoxynucleotidyl transferase ( TdT ) mediated deoxyuridine triphosphate biotin nick end labeling assay , expressions of Bcl 2 and Bax proteins , and caspase 3 enzymatic activity were compared for the different treatment groups . ^^^ The decreased expression of Bcl 2 and the ratio of Bcl 2 to Bax protein seen in cyclosporine treated rat kidneys were significantly increased after colchicine treatment , accompanying a suppression of caspase 3 activity ( P < . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Four molecules of the Bd 2 family ( BID , Bcl 2 , Bax , Bcl 10 ( L ) ) have been reported to be deaved during apoptosis , as has a cellular inhibitor of apoptosis ( XIAP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , it counteracted the effect of TNP BSA on the expression of the Bcl 2 family , resulting in down regulation of Bax and Bad and up regulation of Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to increase the diagnostic specificity , a complex immunohistochemistry panel has been performed consisting of Bcl 2 , Ki 67 , Bax and Pituitary Adenylate Cyclase Activating Peptide ( PACAP ) , somatostatin , VIP and Calcitonin Gene Related Peptide ( CGRP ) . ^^^ The relationship between Bcl 2 and Bax is decisive in generating the final response to the input apoptotic signals . ^^^ We noted a small number of Ki 67 positive cases , which signifies a low mitotic activity of these tumors and a relatively high number of Bax positivities ( 32 . 9 % ) and the much lower number of Bcl 2 positivities ( 11 . 39 % ) , and could explain the benign behaviour of paragangliomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Studies with mouse embryo fibroblasts deficient for the BCL 2 family multidomain proapoptotic proteins BAX and BAK have revealed that both of these proteins are essential for apoptosis induced by multiple stimuli , suggesting that these proapoptotic proteins are functionally overlapping in these cells [ M . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Blood and liver were analyzed for plasma aminotransferase activity , liver histology , liver apoptotic nuclei , mRNA of several cytokines ( tumor necrosis factor [ TNF ] alpha , interleukin [ IL ] 1beta , IL 6 , and IL 10 ) , apoptotic ligands ( TRAIL ) , cytokine receptors ( TNFRp 55 ) , pro and antiapoptotic regulators / adaptors ( Fas receptor , FasL , FADD , TRADD , RIP , Bak , Bax , Bcl 10 , Bcl 2 and Bcl w ) , and caspase 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The presentations were ( 1 ) Mitochondrial regulation of ethanol induced hepatocyte apoptosis : possible involvement of proapoptotic Bcl 2 family protein Bax , by Masayuki Adachi and Hiromasa Ishii ; ( 2 ) Effects of ethanol on mitochondrial reactive oxygen species production and oxidative protein modification , by Shannon M . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Day 20 placentas from viable foetuses were examined for immunoexpression of ( a ) cytokines : tumour necrosis factor alpha ( TNF alpha ) , TNFR 1 receptor ( p 55 ) , leptin and leptin receptor , ( b ) apoptosis : TdT mediated dUTP nick end labelling ( TUNEL ) positive cells , bax and bcl 2 . ^^^ Trophoblast giant cells in VAF placentas exhibited reduced bax but no change in bcl 2 . ^^^ CONCLUSIONS : Maternal vitamin A deficiency is associated with abnormal placental apoptosis induced by neutrophil derived TNF alpha acting through the TNFR 1 ( p 55 ) and / or a change in the bcl 2 / bax ratio in the trophoblast giant cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antisense treatment was associated with an increase in bax and a decrease in bcl 2 secondary to increased synthesis of bax and degradation of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MCF 7 cells were cultured with either 1 nM melatonin , 100 nM D 3 or its diluent to determine their effects on cell proliferation , cell viability , cell cycle phase distribution , population of apoptotic cells , and expression of p 53 , p21WAF1 , bcl 2 , bcl 10 ( L ) and bax proteins . ^^^ No significant changes in bcl 2 , bcl XL and bax mRNAs were detected after treatment with melatonin whereas in D 3 treated cells , a significant drop in bcl XL was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The neuroprotective mcchanisms involve inactivation of NMDA receptors , decreased expression of pro apoptotic proteins , p 53 and Bax , enhanced expression of the cytoprotective protein , Bcl 2 , and activation of the cell survival kinase , Akt . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Reduced expression of both Bax and Bcl 2 is independently associated with lymph node metastasis in human breast carcinomas . ^^^ In this study , we evaluated the expression patterns of Bcl 2 , Bcl xL , and Bax protein in 126 primary invasive breast carcinomas , and the association with other clinicopathological parameters . ^^^ Reduced expression of both Bax and Bcl 2 was associated with lymphnode metastases in univariate analyses ( one way ANOVA ) as well as in multivariate analysis ( binary logistic regression ) ( Bcl 2 p=0 . 003 univariate , p=0 . 01 multivariate , Bax p=0 . 05 univariate , p=0 . 03 multivariate ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed no detectable changes in the steady state levels of Bcl 10 ( L ) , Bax , and Bid , although TNF suppresses Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TUNEL staining detected apoptotic T cells at low frequency corresponding to an increased expression of the anti apoptotic molecules Bcl 2 and Bcl 10 ( L ) and a reduced expression of the pro apoptotic molecules Bad , Bax , and Fas ligand in CD 4 and CD 8 T cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The heterozygotes for p 53 , bcl 2 and bax responded similarly to the wild types , so that no gene dosage effects were identified . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Addition of hydrogen peroxide ( 25 microM ) enhances the amount of bax mRNA , while the level of bcl 2 mRNA remains unchanged . ^^^ These results indicate that hyperthermic pre treatment may exert its anti apoptotic function not only by enhanced expression of constitutive as well as inducible HSPs but also by lowering the level of bax transcripts and thereby increasing the Bcl 2 / Bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the expression of endogenous TIMP 1 , matrix metalloprotein 2 ( MMP 2 ) , and MMP 9 , as well as apoptosis related genes Bcl 2 and Bax were investigated . ^^^ Finally , TIMP 1 over expression inhibited the expression of Bax but with no effect on the expression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This may indicate that p 14 ( ARF ) triggers a so far unknown activator of mitochondrial apoptosis which can be inhibited by Bcl 2 but which acts either independently or downstream of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed no detectable change in the steady state levels of Bax , Bcl 2 , and Bcl 10 ( L ) following raloxifene treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , Bcl 2 and Bax were cleaved in XK 469 treated cells . ^^^ Importantly , in these PA 1 cells expressing DN casp 9 , the activation of caspases including caspases 3 , 7 and 9 , and cleavage of Bax and Bcl 2 were inhibited , suggesting that the activation of the mitochondrial pathway is required for XK 469 induced anticancer activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the presence or absence of bcl 2 and bax was determined by Western blot analysis of control , 6 weeks of denervation , and 10 weeks of denervation specimens . ^^^ Western blot analysis revealed that adherent satellite cells from all groups expressed both bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , activin showed a weak , but significant , mitogenic effect on HEC 50 cells without modifications in Bax and Bcl 2 mRNA and protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The flavones also increased the content of Bcl 2 in the cell , resulted in its phosphorylation , and in contrast decreased the Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased accumulation of p 53 caused expression of Bax , a pro apoptotic member of the Bcl 2 family that is known to cause apoptosis via release of cytochrome c and caspase activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Lithium inhibits aluminum induced apoptosis in rabbit hippocampus , by preventing cytochrome c translocation , Bcl 2 decrease , Bax elevation and caspase 3 activation . ^^^ Intracisternal administration of aluminum into rabbit brain induces cytochrome c release , decreases levels of the anti apoptotic proteins Bcl 2 and Bcl 10 ( L ) , increases levels of the pro apoptotic Bax , activates caspase 3 , and causes DNA fragmentation as measured by the TUNEL assay . ^^^ Pretreatment for 14 days with 7 mm of lithium carbonate in drinking water prevents aluminum induced translocation of cytochrome c , and up regulates Bcl 2 and Bcl 10 ( L , ) down regulates Bax , abolishes caspase 3 activity and reduces DNA damage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect was not the consequence of a change in the protein expression levels of Bcl 2 or Bax induced by lovastatin . ^^^ The cytotoxic activity of lovastatin was higher in Pgp expressing cell lines , but did not correlate with the multidrug resistance ( MDR ) related proteins LRP , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry assay ( IHC ) and Western blot analysis show down regulation of bcl 2 gene and up regulation of bax gene and c myc gene in the treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro apoptotic members of the Bcl 2 family can be subdivided in two classes according to their structure : a group including Bax , Bak , and Bok that display Bcl 2 homology ( BH ) 1 , BH 2 and BH 3 domains and a second group including Bid ( BH 3 interacting domain death agonist ) , Bad , Bim ( Bcl 2 interacting mediator of cell death ) and several others that contain only a BH 3 domain , the BH 3 only proteins . ^^^ Bim ( L ) , although unable to activate Bax , can directly inhibit Bcl 2 or Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neuronal death by apoptosis in the hippocampus was investigated by immunohistochemistry using bcl 2 , bax and caspase 3 antibodies . ^^^ Results of reverse transcriptase polymerase chain reaction analysis showed that bcl 2 and bax mRNA was constitutively expressed in the hippocampus of control rats and showed no significant change in the dexamethasone treated rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
N cadherin intercellular adhesion mediates a 3 . 5 fold increase in Bcl 2 protein expression , whereas the level of the proapoptotic protein Bax remains constant . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further studies demonstrated that the expression of Bcl 2 and Bcl 10 ( L ) decreased in both mRNA and protein levels , whereas p 53 and Bax increased after anti NS 1 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To probe the molecular mechanisms of cellular resistance to apoptosis , melanoma cell lines were analyzed for expression of apoptosis regulators ( apoptotic protease associated factor 1 , FLIP , caspase 8 , caspase 9 , caspase 3 , cellular inhibitor of apoptosis , Bcl 2 , or Bax ) ; no correlation was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Investigation of Bcl 2 , Bax , Fas , Fas ligand ( FasL ) and activated caspase 3 , key regulators of apoptosis that can be modulated by cytokines , revealed complex alterations of mRNA and protein levels in MCP 1 ( / ) and CCR 2 ( / ) mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Components associated with Fas dependent apoptosis ( caspase 3 , bcl 2 and bax ) were characterized using western immunoblot following exposure to serum derived membrane fragments . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the ZS cells , bax and mcl 1 were also approximately 50 % higher compared with ZN controls , whereas bcl 2 mRNA was increased compared with ZA cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated the expression of Mcl 1 , Bcl 2 , Bax , and BAD at 13 21 gestational wk in the human fetal ovary and of Mcl 1 in the adult ovary . mRNA expression of Mcl 1 and its short form Mcl 1s , Bcl 2 , Bax , and BAD was demonstrated in fetal ovary by RT PCR . ^^^ Bax and BAD immunostaining was detected in both somatic and germ cells in the fetal ovary , whereas Bcl 2 was restricted to somatic cells : no changes in expression were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in hypokalemic nephropathy in rats . ^^^ METHODS : To investigate mechanisms of apoptosis in this model , we assessed expression of Bcl 2 and Bax , using immunohistochemistry and reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Bcl 2 protein distributed in the tubules of the OM was significantly decreased in potassium depleted and potassium repleted rats compared with control rats , while immunoreactivity for Bax protein tended to increase above control levels in potassium depleted rats . ^^^ RT PCR for bcl 2 and bax demonstrated a significant decrease in levels of bcl 2 mRNA in potassium depleted and potassium repleted rats relative to those in controls . ^^^ Expression of bax mRNA in potassium depleted and potassium repleted rats tended to increase , while ratios of bcl 2 mRNA to bax mRNA significantly decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Enhanced expression of Bax itself did not inhibit the growth rate of infected cells and did not influence expression of Bcl 2 and Bcl xL . ^^^ Caspase activation and / or an imbalance in Bax and Bcl 2 / Bcl xL expression may be the reasons for the augmentation of cytotoxicity by these drugs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical demonstration of apoptosis regulated proteins , Bcl 2 and Bax , in resected non small cell lung cancers . ^^^ OBJECTIVE : Bcl 2 and Bax proteins regulate apoptosis independently , cooperatively , or both in vitro . ^^^ METHODS : Bcl 2 protein , Bax protein , and spontaneous apoptosis were evaluated retrospectively in 70 resected specimens from NSCLC patients . ^^^ Immunohistochemical ( IHC ) tests were used to assess the expression of Bcl 2 and Bax in the samples . ^^^ No clear associations were found among Bcl 2 expression , Bax expression , and AI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To determine the role of cell proliferation and apoptosis in uterine leiomyoma growth , we studied protein expression of two major regulatory proteins of apoptosis Bcl 2 ( anti apoptotic ) and Bax ( pro apoptotic ) and two endogenous markers of cell replication proliferating cell nuclear antigen ( PCNA ) and Ki 67 in tumors and matched myometrium from premenopausal women . ^^^ Our results showed that the apoptosis regulating proteins ( Bcl 2 and Bax ) were expressed in the cytoplasm of the leiomyoma and myometrial smooth muscle cells throughout the menstrual cycle . ^^^ Bax expression differed from Bcl 2 in that it also was found in the cytoplasm of vascular smooth muscle cells of the myometria and tumors . ^^^ Both tumors and myometrial samples expressed 26 kDa and 21 kDa proteins that reacted with antibodies directed towards Bcl 2 and Bax , respectively . ^^^ Our results suggest that altered apoptosis by overexpression of Bcl 2 or by decreased expression of Bax does not appear to be a major factor in uterine leiomyoma growth . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decreased Bax expression in CTPE was confirmed by Western blots , which also revealed increased expression of anti apoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Monospecific polyclonal antibodies were used for immunohistochemical detection of Bcl 2 , Bax , XIAP , activated ( cleaved ) caspase 3 and 6 , and cleaved DNA Fragmentation Factor 40 ( DFF 40 ) using paraffin embedded tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of key proteins involved in DNA damage recognition ( p 53 ) , cell cycle arrest ( ATM , p 16 and p21 / WAF1 ) and apoptosis ( Bcl 2 and BAX ) were studied . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , NCTD treatment also increased the phosphorylation of Bcl 2 and Bcl 10 ( L ) but did not affect the expression of Bax or Bad . ^^^ Bcl 2 phosphorylation appears to inhibit its binding to Bax since less Bax was detected in immunocomplex with Bcl 2 in NCTD treated HepG 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also analyzed E cadherin , mitogen activated protein kinase isoforms ERK 1 and ERK 2 , Bcl 2 and BAX proteins during the rescue of MCF 7 cells from OA induced cell death , and found that their expression followed temporally defined patterns . ^^^ Cellular levels of E cadherin returned to control levels within the first days of the rescue , followed by ER , ERK 1 , and ERK 2 , and finally by Bcl 2 and BAX proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative analysis of apoptosis and the apoptosis regulatory genes Bcl 2 , Bcl xL , and Bax were performed by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate digoxigenin nick end labeling staining and real time PCR , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 and Bax was examined using reverse transcription polymerase chain reaction and Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The expression of various oncoproteins ( p 21 , EGFR 1 , erbB 2 , c jun , c myc , bcl 2 , Bax ) and suppressor gene products ( WT 1 , RB gene , p 53 ) was studied in normal urothelium , transitional cell carcinoma ( TCC ) cell lines and their MDR sublines by using specific antibodies and an indirect immunofluorescence flow cytometric method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Detailed tissue expression of bcl 2 , bax , bak and bcl 10 in the normal human pancreas and in chronic pancreatitis , ampullary and pancreatic ductal adenocarcinomas . ^^^ METHOD : Expression of bcl 2 , bax , bcl 10 , bak and p 53 was determined in formalin fixed paraffin wax embedded archival specimens of normal pancreatic tissue ( n = 7 ) , chronic pancreatitis ( n = 7 ) , pancreatic ductal adenocarcinoma ( n = 23 ) and ampullary cancer ( n = 7 ) by immunohistochemistry using specific antibodies . ^^^ RESULTS : In normal pancreas and chronic pancreatitis tissues , bcl 2 , bax and bcl 10 were predominantly expressed in ductal epithelial cells while p 53 was not detected . ^^^ In pancreatic ductal adenocarcinoma and ampullary cancer , bcl 2 was not detected compared with expression seen in normal acini ( p < 0 . 01 ) , minor ( p < 0 . 001 ) and major ducts ( p < 0 . 01 ) , bax expression was reduced with respect to minor ducts ( p < 0 . 01 ) but no different from normal acini or major ducts . bak and bcl 10 were more strongly expressed in malignant epithelia compared with acini and major ducts but reduced when compared with minor ducts ( p < 0 . 01 ) . ^^^ CONCLUSION : Pancreatic and ampullary cancer are associated with absent bcl 2 expression . bax , bak and bcl 10 expression was reduced compared with normal minor ducts whilst bak and bcl 10 expression was increased when compared with major ducts . bcl 10 expression correlates with survival following resection and may represent a potential prognosis marker . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ratio between bax and bcl 2 protein increased significantly in the CsA group ( 5 . 3 fold ) , compared with the VH group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In adult rat ventricular myocytes ( isolated with depyrogenated digestive enzymes to minimize tolerance ) , LPS ( 10 ng / ml ) decreased the ratio of Bcl 2 to Bax at 12 h ; increased caspase 3 activity at 16 h ; and increased annexin 5 , propidium iodide , and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling staining at 24 h . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of inducible nitric oxide synthase ( iNOS ) , bcl 2 , bax , and caspase 3 in DMV and NA of rats after right vagotomy was studied . ^^^ RT PCR analysis revealed the enhanced bcl 2 and reduced bax mRNA levels and subsequent up regulation of both bcl 2 and bax mRNA in right sides of the vagotomized brainstems at 1 day and 5 days postoperation , respectively . ^^^ In situ hybridization analysis confirmed the up regulation of bcl 2 and bax mRNA in right DMV and NA of the rats at 5 days following operation . ^^^ Immunohistochemistry analysis showed up regulated Bcl 2 immunoreactivity and undetectable changes in Bax immunoreactivity in DMV and NA of rats at 1 day after vagotomy , whereas enhancement of both Bcl 2 and Bax immunoreactivity was observed at 5 days postoperation . ^^^ Furthermore , our results imply that the apoptosis pathway mediated by Bcl 2 , Bax , and caspase 3 may be activated in vagal motoneurons after right vagotomy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis and immunostaining for alpha smooth muscle actin ( alpha SMA ) , Bax , Bcl 2 , and p 53 were performed . ^^^ A time dependent decrease in Bax protein level and an increase in Bcl 2 protein level were observed in BDL rats treated with Sm 10 . ^^^ Treatment of Sm 10 induced cytoplasmic sequestration of p 53 , downregulation of Bax , and upregulation of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in HA induced apoptosis correlated with a reduction in Bcl 2 , a potent cell death inhibitor , and an increase in Bax protein levels , which heterodimerizes with and thereby inhibits Bcl 2 . ^^^ Both of the antioxidants vitamin C and vitamin E prevented the dysregulation of Bcl 2 and Bax in HA treated endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase activation and IAP , Bcl 2 , Bad , and Bax expression were examined using Western blot analysis , and NF kappaB activation was determined via electrophoretic mobility shift assay ( EMSA ) . ^^^ There was no difference in IAP , Bcl 2 , Bad , and Bax expression between nontransfected , AdlacZ transfected , and AdIkappaB transfected cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The paraffin sections were used to determine apoptotic cells by TdT mediated dUTP biotin nick end labeling method , to identify bcl 2 , bax , fas , fasL and proliferating cell nuclear antigen ( PCNA ) by immunohistochemistry , to demonstrate fas and fasL mRNA by in situ hybridization . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of the AGP at the molecular level was associated with the low protein expression of the c myc and bcl 2 genes and the high protein expression of the p 53 , bax , fas and fas L genes , as well as the cell factor TGF beta ( 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Peptides derived from BH 3 domains of Bcl 2 family members : a comparative analysis of inhibition of Bcl 2 , Bcl 10 ( L ) and Bax oligomerization , induction of cytochrome c release , and activation of cell death . ^^^ Although the precise mechanism of Bcl 2 action remains elusive , current evidence indicates that Bcl 2 inhibits apoptosis by binding and inhibiting pro apoptotic molecules such as Bax . ^^^ Several studies have shown that the BH 3 domains of Bcl 2 and Bax are critically important for Bax / Bcl 2 heterodimerization . ^^^ In this report , we designed and synthesized peptides based on the BH 3 domains of three distinct Bcl 2 family members , Bcl 2 , Bax and Bad . ^^^ In vitro interaction assays were used to compare the abilities of the different peptides to inhibit Bax / Bcl 2 and Bax / Bcl 10 ( L ) heterodimerization , as well as Bcl 2 and Bax homodimerization . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Its biological function can be inhibited by proapoptotic proteins such Bak , Bad , and Bax by forming complexes mediated primarily by the Bcl 2 homology 3 ( BH 3 ) domain . ^^^ By using a competition assay , several peptides derived from the BH 3 region of Bak , Bad , Bax , and Bcl 2 were investigated . ^^^ Bad and Bak BH 3 peptides compete efficiently with IC ( 50 ) values of 0 . 048 and 1 . 14 microM , respectively , while the peptides from the BH 3 region of Bcl 2 and Bax compete weakly . ^^^ The relative binding order of the peptides ( Bad > Bak > Bcl 2 > Bax > mutated Bak ) correlates well with previously published results . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative reverse transcription PCR analysis revealed that the differential expression of BAX , BCL 2 , and caspase 3 genes , specific in rAd p 53 induced apoptotic cells , was not altered in rAd p 21 induced apoptotic cells , suggesting p 21 ( WAF1 / CIP1 ) induced apoptosis through a pathway distinguishable from p 53 induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using reverse transcription polymerase chain reaction , Bcl 10 ( L ) and Bcl 2 were overexpressed in Q versus P AML cells , whereas no difference in Bcl XS and Bax expression was found . ^^^ Furthermore , the Bcl 10 ( L ) / X ( S ) but not the Bcl 2 / Bax ratio was higher in Q AML compared with normal CD34+ Q cells ( P = 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl 2 and bax mRNA by bovine trabecular meshwork cells . ^^^ Proliferation index , apoptosis index , bcl 2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling , flow cytometry and in situ hybridization . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To study the apoptotic death of olfactory receptor neurons in nasosinusitis , we made an inflammation model in rat infected with STAPHYLOCOCCUS : The histochemical changes in olfactory epithelium were examined using antibodies against protein gene product 9 . 5 ( PGP 9 . 5 ) , single strand DNA ( ssDNA ) , Bcl 2 and Bax that might be involved in the apoptosis of olfactory receptor neurons . ^^^ Bcl 2 and Bax were detected only on the infected side in the early stages . ^^^ The Bcl 2 / Bax family seems to play an important role in the apoptosis induced by infection , but not in the apoptosis on the non infected side . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High D glucose significantly increased bax protein , but not bcl 2 , and activated caspase 3 like and 9 , whereas HGF significantly increased bcl 2 expression without affecting bax level and attenuated the increase in caspase 3 and 9 activity . ^^^ Importantly , this bax translocation was also completely blocked by overexpressed bcl 2 . ^^^ These findings suggest that HGF can activate bcl 2 expression and inhibit translocation of bax protein upstream of the mitochondria , thereby leading to the inhibition of caspase 3 and 9 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The effects of olomoucine and bohemine were studied on the following parameters : ( 1 ) cell proliferation , by measurement of DNA content ; ( 2 ) viability , by the MTT ( 3 [ 4 , 5 dimethylthiazol 2 yl ] 2 , 5 diphenyltetrazolium bromide ) and / or XTT ( 2 , 3 bis [ 2 methoxy 4 nitro 5 sulfophenyl ] 2H tetrazolium 5 carboxanilide ) test ; and ( 3 ) the expression of p 53 , pRB , Bcl 2 , Bax , p 16 , p 21 , p 27 , cyclins A , B , D 1 , E , p 34 ( cdc 2 ) , and the androgen receptor ( AR ) , by western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hydroxychloroquine induced apoptosis of chronic lymphocytic leukemia involves activation of caspase 3 and modulation of Bcl 2 / bax / ratio . ^^^ The expression of bcl 2 and bax proteins was significantly modified after incubation with the drug and HCQ activity against CLL cells occurred independently of the presence of IL 4 , sCD40L and bone marrow stromal cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Expression of Bcl 2 , Bax , and Ki 67 was immunohistochemically investigated in both normal skin and BCC cells , as well as in the epidermis overlying BCC . ^^^ In BCC , cells stained homogeneously for Bcl 2 , whereas Bax and Ki 67 showed scattered staining patterns . ^^^ Simultaneous expression was seen for Bcl 2 and Bax in 80 + / 7 % of the tumor cells , and co expression of Bcl 2 and Ki 67 in 20 + / 7 % of the tumor cells . ^^^ The cells expressing Bcl 2 and Ki 67 , but lacking expression of Bax , the progressive fraction , comprised on average 7 + / 3 % of the tumor cell population . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The opposite action of anti apoptotic proteins ( Bcl 2 family ) and pro apoptotic proteins ( p 53 , Bax , Bak ) regulates the activation of caspases that are the effectors proteases of the cell suicide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Department of Veterans Affairs Cooperative Study Program ( VA CSP 268 ) were evaluated for the expression of Bcl 2 , Bcl 10 ( L ) , and Bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We studied the dynamic changes of apoptosis of mouse thymus lymphocytes and the expression of bax and bcl 2 gene products after 2 , 4 , 6 and 8 Gy of whole body gamma irradiation using in situ terminal labeling , DNA electrophoresis and immunohistochemical techniques . ^^^ Abnormal expression of bcl 2 and bax gene products were shown in irradiated lymphocytes . ^^^ Bcl 2 and Bax proteins may regulate the process of lymphocyte apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examinations by the TUNEL method and immunohistochemistry using anti Fas , Bax , and Bcl 2 antibodies were conducted . ^^^ Bax and Fas were expressed on the remaining liver cells , while Bcl 2 was seen on the infiltrating lymphocytes . ^^^ The frequency of TUNEL positive liver cells showed a significant correlation with Bax expression and the grades of inflammatory cell infiltration , but a poor correlation with Fas and Bcl 2 expression . ^^^ Fas and Bcl 2 might not be involved in the apoptosis of liver cells , but the findings suggested that Bax might play a role in inducing liver cell apoptosis , though the mechanism could not be explained . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The changes in mitogen induced proliferation in DES treated female mice were not mirrored by similar changes in the relative numbers of CD 90 ( + ) or CD45R ( + ) cells , or in ratios of anti apoptotic Bcl 2 to apoptotic Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of p 53 and Bax proteins were elevated , while Bcl 2 protein was downregulated in TCE or PERC treated p 53 WT H 460 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Investigation of bax , bcl 2 , bcl 10 and p 53 gene polymorphisms in multiple sclerosis . ^^^ To analyze genetic differences in the apoptosis regulating factors bcl 2 , bax , bcl 10 and p 53 we investigated polymorphisms of these genes in 105 patients with a relapsing remitting disease course and 99 controls by PCR SSCP and direct sequencing . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The involvement of apoptosis in these slices was suggested by using the terminal transferase mediated biotinylated UTP nick end labeling ( TUNEL ) technique , and immunohistochemistry for the apoptosis related markers Bax and Bcl 2 . ^^^ In 3 NP exposed slices , the pro apoptotic protein Bax was abundantly expressed , whereas the anti apoptotic protein Bcl 2 was not expressed in striatal neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the prognostic significance of p 21 ( WAF1 / CIP1 ) expression and its relationship with p 53 accumulation and other apoptotic markers such as Bax , Bcl 2 , and apoptotic index in relation to disease specific survival in oral tongue squamous cell carcinoma ( SCC ) . ^^^ RESULTS : Expression of p 21 ( WAF1 / CIP1 ) correlated inversely with T classification and clinical stage ( P = . 02 and P = . 006 , respectively ) but not with N classification , tumor differentiation , or apoptosis related variables such as p 53 accumulation , apoptotic index , and Bax and Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Anti apoptotic effect of succinyl gelatine in a liver ischaemia reperfusion injury model ( Bcl 2 , Bax , Caspase 3 ) . ^^^ The expression of Bcl 2 , Bax and caspase 3 were analysed by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , it was shown that ethanol induces increases in levels of bax and caspase 3 and a decrease in bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis in normal rat embryo tissues during early organogenesis : the possible involvement of Bax and Bcl 2 . ^^^ In this study , we investigated the relationship between apoptosis and the expression of both Bax and Bcl 2 during the early organogenesis period ( 9 . 5 11 . 5 days of gestation ) of rat embryos . ^^^ Real time RT PCR analysis of whole embryos revealed that the expression of bax mRNA was constant at days 9 . 5 to 11 . 5 , while the expression of bcl 2 mRNA gradually increased . ^^^ Immunohistochemical studies of Bax and Bcl 2 expression revealed that these apoptotic cells were exactly positive to Bax in mirror sections , while their expression of Bcl 2 was generally too low to be detected . ^^^ It was similarly observed that apoptotic cells detected in the cardiogenic area at 9 . 5 days of gestation disappeared with the formation of the primitive heart tube accompanied by a strong expression of both Bcl 2 and Bax in the developmental process of the primitive heart . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied proliferation by counting mitotic figures and by MIB 1 labeling , apoptosis by TUNEL and expression of proteins related to cell cycle regulation by immunohistochemical analysis of p 53 , p 21 , bcl 2 and bax expression in 48 low grade gliomas . ^^^ No significant differences were observed in MIB LI , TUNEL LI , bcl 2 and bax expression . ^^^ In the gliomas without radiotherapy ( n = 15 ) , no differences in mitotic rate , TUNEL LI , p 53 , p 21 , bcl 2 and bax expression were found between primary tumors and their recurrences . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human Meissner corpuscles express Bcl 2 but not Bax protein . ^^^ Bcl 2 and Bax proteins play major roles in the control of apoptosis . ^^^ The Bcl 2 / Bax ratio is considered a marker of a cell ' s susceptibility to apoptotic stimuli . ^^^ Immunohistochemical expression of Bcl 2 and Bax in Meissner corpuscles was investigated in 30 human skin samples . ^^^ All of the Meissner corpuscles showed immunoreactivity for Bcl 2 and Bax was negative . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We aimed to determine bcl 2 and bax gene expression in oral epithelial dysplasias ( OED ) in relation to apoptosis and proliferation . ^^^ Samples of normal oral epithelium ( OE , n=7 ) , focal epithelial hyperplasia ( n=9 ) , mild ( n=9 ) , moderate ( n=8 ) and severe ( n=18 ) OED were evaluated by immunohistochemistry , the TUNEL method and in situ hybridisation for bcl 2 and bax mRNA . bcl 2 mRNA and protein were markedly decreased in the basal parts of moderate and severe OED compared with the basal layer of OE ( P < 0 . 001 ) and correlated with a 3 4 fold increase in apoptosis and increased proliferation . bax mRNA and protein were not significantly altered in normal , hyperplastic and dysplastic epithelia . ^^^ From OE to severe OED , there was an inverse relationship between the bcl 2 / bax ratio and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative analysis of expression levels of bax , bcl 2 , and survivin in cancer cells during cisplatin treatment . ^^^ In the present study , to evaluate good markers of chemo sensitivity or chemo resistance of cancer cells , the correlation between occurrence of apoptosis and the changes in expression levels of messenger RNA ( mRNA ) of three genes ( bax , bcl 2 , and survivin ) in cancer cell lines during CDDP treatment were investigated . ^^^ The changes in expression levels of three genes ( bax , bcl 2 , and survivin ) during CDDP treatment were evaluated by real time reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ These findings indicate that during chemotherapy , changes in expression levels of bax , bcl 2 , and survivin may provide information about chemo sensitivity or the chemo resistance of tumors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Incubation with IC 50 concentrations of antisense ferritin ODNs caused reduction in cell volume , condensation of nuclear structures and lower levels of Bcl 2 mRNA and protein compared to control cells , but Bax mRNA and protein levels remained unchanged . ^^^ Further , the antisense ferritin ODNs promote programmed cell death with low ratios of Bcl 2 to Bax mRNA and protein expression providing evidence that antisense ferritin ODNs specifically inhibit MCF 7 breast carcinoma cell growth through increased apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High bad and bcl xL gene expression and combined bad , bcl xL , bax and bcl 2 mRNA levels : molecular predictors for survival of stage 2 soft tissue sarcoma patients . ^^^ Samples from 82 STS patients were analyzed for mRNA expression of bad , bax , bcl xL and bcl 2 by a high throughput quantitative RT PCR approach , using validated assays based on TaqMan technology . ^^^ Considering STS patients of tumor stage 2 , multivariate Cox analysis revealed that bad mRNA values > or = 10 ( p=0 . 0039 ; RR=9 . 08 ) , bcl xL > or = 1 . 5 ( p=0 . 067 ; RR=4 . 59 ) , bax > or = 0 . 005 ( p=0 . 1 ; RR=2 . 84 ) and bcl 2 < 3 ( p=0 . 42 ; RR=1 . 7 ) were associated with a poor prognosis . ^^^ There was a 14 . 5 fold and 6 . 5 fold increase in the risk for the combinations of high bax / bcl xL mRNA ( p=0 . 018 ) and bax / bcl 2 mRNA expression ( p=0 . 017 ) , respectively . ^^^ In conclusion , high bad mRNA levels and combined values of bad / bcl xL bax / bcl xL and bax / bcl 2 appear to be independent prognostic factors at least for stage 2 STS patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Momordin 1 treatment also gradually decreased the expression of . the anti apoptotic protein Bcl 2 , but increased the expression of the pro apoptotic protein Bax . ^^^ In this study we showed that momordin 1 induced apoptosis of HL 60 cells by reduction of the Bcl 2 : Bax ratio and by activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a proapoptotic member of the Bcl 2 family of proteins , resides in the cytosol and translocates to the mitochondrial membrane upon induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A recombinant adenovirus expressing wild type Bax induces apoptosis in prostate cancer cells independently of their Bcl 2 status and androgen sensitivity . ^^^ The adenoviral co transfection system induced Bax protein expression and apoptosis even in these Bcl 2 transfected cell lines . ^^^ Taken together , our results suggest that this Bax expression system might represent a useful gene therapy strategy when applied to the treatment of prostate cancer and its efficacy would be independent of the Bcl 2 status and androgen sensitivity of these cancers . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For this we studied the effect of CA4P on selected markers of apoptosis [ caspases 9 and 3 , poly ( ADP ribose ) polymerase ( PARP ) , bcl 2 , and bax ] and G 2 M protein regulators ( p 53 , MDM 2 , 14 3 3sigma , GADD 45 , cdc 2 , cdc 25 , chk 1 , wee 1 , p 21 , and cyclin B 1 ) . ^^^ No change in bcl 2 or bax protein expression was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax in chewing tobacco induced oral cancers and oral lesions from India . ^^^ To investigate the involvement of apoptosis regulating proteins in oral cancer in Indian patients , primarily associated with chewing tobacco habits , immunohistochemical expression of bcl 2 and bax was examined in 63 oral squamous cell carcinomas , and 31 putative premalignant lesions . ^^^ Our studies revealed overexpression of tumor specific cytoplasmic bcl 2 in 56 % and bax in 43 % oral cancers . ^^^ The oral cancers in the Indian patients are preceded by premalignant oral lesions ; hence oral lesions were examined for bcl 2 and bax expression . ^^^ We observed aberrant expression of bcl 2 in 16 % oral lesions comprising leukoplakias and SMF and bax in 55 % oral lesions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While a significant decrease in bcl 2 , an anti apoptotic molecule , occurred in the cells overexpressing mutant PS 2 , no significant change was observed in bax , a pro apoptotic molecule , as compared with the cells overexpressing wild type PS 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the end of the experiment , the whole CNS of each animal was collected for histopathology and immunohistochemistry for apoptotic markers ( BAX , BCl 2 and CPP 32 ) and for glial fibrillary acid protein ( GFAP ; vimentin ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The role of Bax and Bak , 2 proapoptotic proteins of the Bcl 2 family , was analyzed in primary B cell chronic lymphocytic leukemia ( CLL ) cells following in vitro treatment with fludarabine , dexamethasone , or the combination of fludarabine with cyclophosphamide and mitoxantrone ( FCM ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Paraffin embedded archival tissue from 103 N0M0 consecutive patients with invasive bladder cancer ( 28 T 1 , 57 T 2 , 13 T 3 and 5 T 4 ) was immunostained for bcl 2 , bax , bcl XL , bcl Xs , p 53 , Ki 67 and with an anti single stranded DNA monoclonal antibody recognizing the apoptotic cells . ^^^ RESULTS : Most tumours were immunoreactive for bax ( 73 . 1 % ) and bcl XL ( 80 . 9 % ) whereas bcl 2 and bcl XS expression was comparatively less common ( 44 . 4 and 28 . 9 % , respectively ) . ^^^ On the contrary , bax and bcl 2 positivity was unrelated to stage or grade . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DEX increases the expression of anti apoptotic Bcl 2 and Bcl 10 ( L ) proteins , decreases the expression of pro apoptotic Bax and inhibits Bad translocation thereby preventing the release of cytochrome c , the activation of caspases , and cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis , as well as increased mitochondrial efficiency , superoxide dismutase activity , and Bcl 2 : Bax ratio . ^^^ In addition to adaptations to antioxidant defenses , other adaptive mechanisms occurred in the mitochondria such as an increase in the respiratory P / O ratio and an increase in the Bcl 2 : Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results indicated a `` two step ' ' mechanism of the induction of apoptosis in tumor cells , by PA , i . e . ( 1 ) activation of the immune system of the host to release different apoptogenic factors like tumor necrosis factor alpha ( TNF alpha ) and nitric oxide ( NO ) ; and ( 2 ) induction of EAC apoptosis by these soluble immune mediators through the up regulation of pro apoptotic factors ( p 53 and Bax ) and down regulation of anti apoptotic factor ( Bcl 2 ) , resulting in the activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In response to various apoptotic stimuli , Bax , a pro apoptotic member of the Bcl 2 family , is oligomerized and permeabilizes the mitochondrial outer membrane to apoptogenic factors , including cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , in Bcl 2 transfected cells Bak activation was also blocked , while Bax was still partially active in agreement with differential regulation of these two proteins . ^^^ This enhanced apoptosis was preceded by enhanced Bak and Bax activation , and both responses as well as apoptosis were blocked in transfectants overexpressing Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Based on the genes studied , we could propose a general pathway for the cell reaction according to the surface treatments used : ( 1 ) metal ion release changes the time course of gene expression in the FAK pathway ; ( 2 ) once the accumulation of metal ions released from the Ti surface exceeds a threshold value , cell growth is diminished and apoptosis may be activated ; ( 3 ) PTK up regulation is also induced by metal ion release ; ( 4 ) the expression of Bcl 2 family and Bax may suggest that metal ions induce apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed down regulation of c Myc and of the anti apoptotic proteins Bcl 2 and Bfl 1 as well as high levels of the pro apoptotic proteins Bax and Bid . ^^^ Conversely , cells stably expressing NTD MR exhibited increased expression of Bcl 2 and Bfl 1 and diminished levels of Bid and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the intracellular level , prolonged morphine administration resulted in an upregulation of the proapoptotic caspase 3 and Bax proteins but a downregulation of the antiapoptotic Bcl 2 protein in the spinal cord dorsal horn . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for Fas , Bcl 2 , Bax , Bcl xl , inducible nitric oxide synthase ( iNOS ) , and cyclooxygenase 2 ( COX 2 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
For this reason , we monitored the pattern of expression of two members of the Bcl 2 family , Bcl 2 and Bax , in control and vasoactive intestinal peptide treated specimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 homology ( BH ) 3 only pro apoptotic Bcl 2 family protein Bim plays an essential role in the mitochondrial pathway of apoptosis through activation of the BH 1 3 multidomain protein Bax or Bak . ^^^ Taken together , these findings indicate that BimEL may activate Bax by damaging the mitochondrial membrane structure directly , in addition to its binding and antagonizing Bcl 2 / Bcl XL function . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Negative correlation between the ratio of Bax to Bcl 2 and the size of tumor treated by culture supernatants from Kupffer cells . ^^^ The levels of Bax , Bcl 2 and iNOS were analyzed in an in vivo mouse tumor model , which was treated with culture supernatant from activated Kupffer cells . ^^^ The results showed that the expression of Bax significantly increased while the expression of Bcl 2 decreased when tumor cells were treated with culture supernatants from activated Kupffer cells . ^^^ The alteration of Bax and Bcl 2 levels resulted in an increase in the ratio of Bax to Bcl 2 , which had negative correlation with the size of tumor and positive correlation with the expression of iNOS . ^^^ In conclusion , culture supernatants from activated Kupffer cells were able to change the balance between Bax and Bcl 2 in favor of the former . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
LOX inhibitor treatment also reduced the levels of anti apoptotic proteins Bcl 2 and Mcl 1 and increased the levels of the pro apoptotic protein bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Evidence has emerged to suggest that Bcl 2 , Bax , JNK , and caspases are implicated in neurotoxic effects due to in vivo MPTP administration to mice . ^^^ Different neuronal cell lines such as MN9D cells , SH SY5Y cells , cerebellar granule neurons , cortical neurons , and GH 3 cells were also tested to investigate the possible involvement of Bcl 2 , Bax , and caspases in in vitro MPP ( + ) induced neurotoxicity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In drug sensitive 32D parental and low Bcr Abl expressing cells , pro apoptotic Bcl 2 family members , Bax and Bad translocate from the cytosol to the mitochondrion following a cytotoxic insult . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of proapoptotic BAX and BAD and anti apoptotic BCL 2 was examined in single embryos using RT PCR and immunohistochemistry . ^^^ BAX and BCL 2 mRNAs were expressed throughout development , whereas BAD mRNA was expressed mainly after compaction . ^^^ Simultaneous expression of BAX and BCL 2 proteins within individual embryos was confirmed using immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 40 ligation and TNF alpha prevented release of cytochrome c and activation of caspase 3 , which could not be explained by effects on the expression of Bcl 2 , Bcl 10 ( L ) or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To analyze the mechanisms of the apoptotic activity of Cl F araA , we sought to determine the effects of the drug on the levels of Bcl 2 family proteins ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , Bak ) and cell survival signals via Akt . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes ( mRNA , protein ) in expression of major proapoptotic p 53 , p21WAF1 / CIP1 , bax , bcl Xs genes , and the antiapoptotic bcl 2 gene were observed in malignant MCF 7 and MDA MB 231 cells and in benign MCF 10a human mammary tumor cells in culture . ^^^ Apparent antiapoptotic effects of increased bcl 2 expression in MDA MBA 231 cells were countered by increased proapoptotic p21WAF1 / CIP1 , Bax , and Bcl Xs proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ribonuclease protection assay was used to investigate the expression of selected apoptosis related genes of the Bcl 2 family , pro apoptotic Bax , Bad and anti apoptotic Bcl 2 , during differentiation of murine embryonic stem cells ( ES ) mediated by all trans retinoic acid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The up regulation of bax and bcl 2 , both members of the bcl 2 family , indicate that the predominant effectors involve permeability transition pores in the mitochondrial membrane and subsequent caspase activation which confers the typical morphological and biochemical features of apoptosis such as DNA fragmentation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Image cytometric bcl 2 : bax and bcl 2 : bcl 10 ratios in invasive breast carcinoma : correlation with prognosis . ^^^ Some of the members , such as bcl 2 and bcl 10 ( L ) , inhibit cell death , whereas others , such as bax and bcl 10 ( S ) , promote cell death . ^^^ We evaluated the ratios of bcl 2 : bax and bcl 2 : bcl 10 expression by image cytometry in invasive breast carcinoma to determine prognostic significance . ^^^ DESIGN : Five micron sections of formalin fixed , paraffin embedded tissue from 88 invasive breast carcinomas were immunostained using steam antigen retrieval , an avidin biotin complex technique with automated stainer and primary antibodies against bcl 2 ( 1 / 160 ; Dako , Carpenteria , CA ) , bax ( 1 / 1 , 500 ; PharMingen , San Diego , CA ) , and bcl 10 ( 1 / 1 , 500 ; PharMingen ) . ^^^ Positive controls were tonsil ( bcl 2 ) and normal breast ( bax and bcl 10 ) tissue samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Attention has been focused on the connections between nuclear PKC delta activation and the expression of cell death regulators ( Bcl 2 family proteins Bad , Bax and Bcl 2 ) and cell death effector caspase 3 ( CPP 32 ) which lead to the cleavage of cytoskeletal and nuclear proteins and induction of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have investigated whether subsets of indolent B cell non Hodgkin ' s lymphoma ( IB NHL ) differ in the expression of the bcl 2 family members ; 116 cases of IB NHL , composed of chronic lymphocytic leukemia ( CLL , n = 48 ) , follicular lymphoma ( FL , n = 38 ) , marginal zone B cell lymphoma ( MZBCL , n = 15 ) , and mantle cell lymphoma ( MCL , n = 15 ) , were investigated for expression of bcl 2 , bcl 10 , mcl 1 , bax , and bak proteins by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of PC 12 cells was also assessed by PARP cleavage detection , Western blot analysis of Bax and Bcl 2 proteins , and DNA laddering on agarose gel electrophoresis . ^^^ The proapoptoic protein Bax was dominantly expressed , whereas Bcl 2 was slightly down regulated by SAM . ^^^ SAH weakly induced the expression of Bax and slightly decreased Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , the down regulation of bcl 2 protein by ectopic PAR 4 expression altered bcl 2 : bax ratio in PC 3 / PAR 4 cells and this led enhanced radiosensitivity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Total endothelial cell growth factor deprivation gave a significant increase in apoptosis accompanied by a decrease of Bcl 2 in apoptotic cells while Bcl xl and Bax levels were unaffected . ^^^ Our data indicate that anti apoptotic protein Bcl 2 and pro apoptotic protein Bax are reciprocally regulated during apoptosis , whilst Bcl xl is essentially unaffected . ^^^ This implies that Bcl 2 / Bax ratio rather than Bcl xl controls apoptosis in primary endothelial cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the role of nitric oxide ( NO ) and death regulatory genes , bcl 2 and bax , in human endometria apoptosis . ^^^ DESIGN : Expression of bcl 2 , bax , NO synthases ( NOS ) , and the apoptotic effect of L arginine on endometrial explants in vitro . ^^^ Immunostaining of Bcl 2 was almost absent in the secretory endometria , whereas Bax increased in the stroma at the end of the menstrual cycle , coincident to the decrease in the bcl 2 / bax mRNA relative ratio ( P < . 05 ) observed in secretory endometria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AT1R and AT2R protein expression ( Western immunoblot ) , CM apoptosis ( terminal deoxynucleotidyl transferase mediated 2 ' deoxyuridine 5 ' triphosphate nick end labelling assay and nuclear morphology ) and apoptotic markers ( Bax , Bcl 2 , caspase 3 , p 53 ) were assessed in LV tissue . ^^^ RESULTS : Compared with IR controls , CN improved peak systolic pressure , LV developed pressure and positive dp / dt , and increased AT2R ( not AT1R ) protein , but did not change the level of apoptosis or the expression of Bax , Bcl 2 , caspase 3 or p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A survey of molecules involved in TRAIL or Bax mediated apoptotic pathways showed no significant change in expression of death receptors , death decoy receptors ; FLIP ; Bcl 2 ; Bcl xS ; Bax ; Bak ; XIAP or caspase 2 , 7 , 8 , or 9 in either DLD1 / Bax R or DLD1 / TRAIL R cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bcl 2 , and p 53 expression in endometrial cancer . ^^^ BACKGROUND : It has not been fully clarified whether alteration of Bax and other apoptosis relating proteins of Bcl 2 and p 53 is involved in endometrial carcinogenesis . ^^^ METHODS : A total of 56 frozen tissues , which included 14 normal endometria , 13 endometrial hyperplasias ( 10 without atypia and 3 with atypia ) , and 29 endometrial carcinomas , were examined for the expression of Bax , Bcl 2 , and p 53 using immunohistochemistry . ^^^ RESULTS : Both Bax and Bcl 2 were distinctly expressed in the normal proliferative phase endometrium . ^^^ A decreased Bcl 2 / Bax ratio in the secretory phase endometrial gland cells due to suppressed Bcl 2 expression was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , Rho inhibition reduced expression of antiapoptotic Bcl 2 and Mcl 1 and increased proapoptotic Bid but had no effect on Bax or FLIP protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , bcl 2 suppresses both bax and rpr induced mitochondrial defects while the caspase inhibitor p 35 is specific to the rpr pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of dl praeruptorin A on interleukin 6 level and Fas , bax , bcl 2 protein expression in ischemia reperfusion myocardium . ^^^ Apoptosis related protein Fas , bax , and bcl 2 expression was detected by immunohistochemistry and computer image analysis system . ^^^ RESULTS : Pd Ia 2 . 0 mg / kg 4 lowered serum IL 6 level and Fas , bax , bcl 2 expression under conditions with hypotension and without changes on heart rate , but increased the ratio of bcl 2 / bax . ^^^ There existed a close linearity and positive correlation between IL 6 level and Fas , bax , bcl 2 expression . ^^^ The reason might be associated with modulating the expression of some immediate early genes including IL 6 , Fas , bax , and bcl 2 in ischemia reperfusion myocardium . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immunohistochemistry assay was used to detect the protein levels of Bcl 2 , Bcl xl , Bax , inducible nitric oxide synthase ( iNOS ) , neuronal NOS ( nNOS ) , and cleaved caspase 3 . ^^^ Moreover , Rg 1 elevated the levels of cleaved caspase 3 , Bax , and iNOS , but reduced the levels of Bcl 2 and Bcl xl ( P < 0 . 01 ) . ^^^ CONCLUSION : Rg 1 has protective effect against MPTP induced apoptosis and this effect may be attributed to enhancing Bcl 2 and Bcl xl expression , reducing Bax and iNOS expression , and inhibiting activation of caspase 3 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax immunoreactivity were examined in the spinal nucleus of the bulbocavernosus ( SNB ) , an androgen sensitive motor pool of adult rats . ^^^ Hormone manipulations did not affect Bcl 2 or Bax staining in the retrodorsolateral nucleus ( RDLN ) , a relatively androgen insensitive nucleus at the same spinal level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased Bcl 2 and reduced Bax expression in infected macrophages in slowly progressive primary murine Mycobacterium tuberculosis infection . ^^^ In vitro studies have shown that MTB can induce and inhibit apoptosis by causing the expression of Bax and Bcl 2 , respectively , suggesting that the infected cells ' fate depends on pro and antiapoptotic signals . ^^^ The aim was to study the pattern and distribution of Bcl 2 and Bax in cellular infiltrates of MTB infected B6D2F1 hybrid mice and correlate the expression with the presence of MTB antigens ( MAgs ) . ^^^ Using formalin fixed lung tissues ( n = 45 ) , our results showed a significant difference in the percentage of Mphis stained for Bcl 2 or MAgs and Bax ( P < 0 . 0001 ) . ^^^ Bcl 2 expression was increased in a population of Mphis and corresponded in intensity , colocalization and percentage with that of MAgs on the same cells , while Bax expression was reduced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell survival indices including proliferating cell nuclear antigen ( PCNA ) , Bcl 2 , Bcl xL and Bax were measured by immunohistochemistry and Western blots . ^^^ Bcl 2 was enhanced in the HGF transfected UUO rats , while no changes of Bcl xL and Bax were found . ^^^ Bcl 2 rather than Bcl xL or Bax may contribute to the anti apoptotic function of HGF . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A shift in the Bax / Bcl 2 balance may activate caspase 3 and modulate apoptosis in experimental glomerulonephritis . ^^^ Of the known regulators of apoptosis , the best characterized is the Bax to Bcl 2 ratio . ^^^ Here , using the nephrotoxic nephritis ( NTN ) model , we evaluated Bax / Bcl 2 in relation to changes in the apoptosis co ordination enzyme , caspase 3 . ^^^ These were analyzed for apoptosis ( in situ end labeling of fragmented DNA , light and electron microscopy ) , Bax / Bcl 2 protein ( Western blotting ) , mRNA ( Northern blotting ) and distribution ( immunohistochemistry ) , as well as caspase 3 activity ( substrate cleavage assay ) , inflammation ( ED 1 staining ) , proliferation ( proliferating cell nuclear antigen staining ) and fibrosis ( Masson ' s Trichrome staining ) . ^^^ RESULTS : Bax mRNA was significantly increased while that of Bcl 2 was decreased throughout the time course ( +265 % and 62 % by day 45 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and apoptosis related factors Bcl 2 , Bax , tumor necrosis factor alpha , and NF kappaB in human endometrial hyperplasia and carcinoma . ^^^ Bcl 2 and Bax were expressed in normal and hyperplastic endometrium , and the Bcl 2 / Bax ratio was lower in endometrial carcinoma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Indeed , the antiapoptotic protein Bcl 2 was augmented in these same areas , with no change in the proapoptotic protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and BAX protect adult mice from lethal Sindbis virus infection but do not protect spinal cord motor neurons or prevent paralysis . ^^^ Both endogenous and overexpressed BCL 2 and BAX proteins protect newborn mice from fatal SV infection by blocking apoptosis in infected neurons . ^^^ This vector has allowed assessment of the effects of BCL 2 and BAX on both mortality and paralysis in these hosts . ^^^ Similar to newborn hosts , weanling mice infected with dsNSV encoding BCL 2 or BAX survived better than animals infected with control viruses . ^^^ This finding indicates that BCL 2 and BAX both protect neurons that mediate host survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No correlation was found between apoptosis sensitivity and the expression of TNF receptor 1 or the expression of Bax , Bcl 2 and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The induction of apoptosis by GEN was associated with a 52 . 8 % decrease ( p=0 . 001 ) in the immunoreactivity to antiapoptotic Bcl 2 and with a 195 . 9 % ( p=0 . 001 ) increase in the immunoreactivity to proapoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis regulating factors Bcl 2 , Bax and TNF alpha , transcription factor NF kappaB and Caspase 3 , a key executioner protein in apoptotic cell death , were studied by immunohistochemistry and in situ hybridisation . ^^^ The apoptosis regulating proteins Bcl 2 and Bax were expressed constantly in the CL throughout the luteal phase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The `` BH 3 only ' ' proteins of the BCL 2 family require `` multidomain ' ' proapoptotic members BAX and BAK to release cytochrome c from mitochondria and kill cells . ^^^ Another subset characterized by the BH 3 peptides from BAD and BIK can not directly activate BAX , BAK but instead binds antiapoptotic BCL 2 , resulting in the displacement of BID like BH 3 domains that initiate mitochondrial dysfunction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cellular responses to TF induced apoptosis were demonstrated to be associated with the p 53 signaling pathway , including induction of p 53 , p21 / Cip1 , p27 / Kip1 , bax protein expression and inhibition of bcl 2 protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , immunoreactivities detection of Bcl 2 ( which promotes cell survival ) , Bax ( which promotes cell death ) and P 53 ( product of tumor suppressor gene p 53 ) showed that the combination group apparently attenuated P 53 immunoreactivities expression whereas augmented the immunoreactivities expression ratio of Bcl 2 to Bax in cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of p 53 , Bcl 2 , Bax , and Fas proteins in squamous cell carcinomas from immunosuppressed renal transplant recipients and immunocompetent individuals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis after COX 2 inhibition with SC 58635 ( 50 micromol / L ) was independent of BCL 2 , BAX , and the phosphorylation status of AKT / PKB and BAD , but correlated with activation of caspase 9 , caspase 3 , and caspase 6 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Likewise , no changes were found in mRNA levels of the apoptosis related genes bcl 2 and bax in any of 24 brain regions examined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 437 mediated apoptosis was not associated with the modulation of Bcl 2 , Bax , or Mcl 1 levels , but was associated with the cleavage of the antiapoptotic protein Bcl 10 ( L ) to a proapoptotic 18 kD form . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic BAD ( Bcl 2 associated death protein ) has been shown to dissociate from its sequestered site with the molecular chaperone protein 14 3 3 and displace proapoptotic BAX ( Bcl 2 associated 10 protein ) from antiapoptotic BCL Xl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analyses of bcl 2 , bax , bcl 10 , and steroid receptors were performed in 22 endometrial carcinomas , 26 endometrial hyperplasias , and 19 cases of normal cyclical endometrium . ^^^ In hyperplasias , there was a significant positive correlation between bcl 2 and PR and between bcl 2 and bax and a negative correlation between ER and bax . ^^^ There was a statistically significant difference for bcl 2 ( p = 0 . 001 ) and bax ( p = 0 . 001 ) between the hyperplasia and carcinoma groups . ^^^ There was increased expression of bax , decreased expression of bcl 2 , and persistence of bcl 10 protein in advanced endometrial carcinomas . ^^^ Our findings show that ovarian hormones have a regulatory role on bcl 2 protein and that there is a correlation between other members of the bcl 2 family ( bcl 10 and bax ) and steroid hormone receptors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was carried out in 58 / 74 carcinomas by using a panel of monoclonal and polyclonal antibodies against estrogen receptor ( ER ) , progesterone receptor ( PR ) , HER 2 / neu , Bcl 2 , Bax , Fas and DNA fragmentation factor ( DFF ) . ^^^ Statistically significant associations were also found between malignant appearing microcalcifications and HER 2 / neu positivity ( p < 0 . 01 ) , Bax positivity ( p < 0 . 01 ) , Fas positivity ( p < 0 . 05 ) and DFF positivity ( p < 0 . 01 ) , whereas no statistical correlation was found with Bcl 2 positivity ( p > 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ischemia reperfusion alone caused contractile dysfunction without significant myocardial necrosis ( left ventricular pressure volume curves ; 1 % triphenyltetrazolium chloride staining ; creatine kinase release ) or apoptosis ( terminal deoxynucleotidyl transferase mediated nick end labeling [ TUNEL ] analysis ; immunoblotting for poly ( ADP ribose ) polymerase [ PARP ] cleavage ; activation of caspases 3 , 8 , and 9 ; expression of Bax / Bcl 2 ) . ^^^ Dopamine also increased cellular content of pro apoptotic Bax while decreasing anti apoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , Trp P 1 upregulated anti apoptotic factors Bcl 2 and Bcl XL and downregulated pro apoptotic factor Bax in mitochondria 1 hr after injection , indicating that Trp P 1 also stimulated anti apoptotic signals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In CLL cells taken from 37 previously treated and untreated CLL patients , we investigated the influence of bendamustine alone , and in combination with fludarabine , on the induction of apoptosis and changes of Bcl 2 and Bax expression on mRNA and protein level using the RNase protection assay or flow cytometry , respectively . ^^^ The level of the initial Bcl 2 and Bax protein and the m RNA expression remained unchanged during the incubation with bendamustine . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Corticosterone differentially regulates bax , bcl 2 and bcl 10 mRNA levels in the rat hippocampus . ^^^ It is concluded that the up regulation of bax may explain the apoptosis observed in DG after ADX , while the bcl 2 induction may correspond to a compensatory mechanism protecting the cells from death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Progesterone , oestradiol , androstenedione and testosterone concentrations were determined by radioimmunoassay ; caspase 3 expression was assessed by immunohistochemistry ; bcl 2 , bax and P ( 450arom ) expression were assessed by RT PCR ; and apoptosis was detected by in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labelling . ^^^ This action may be direct or mediated by steroid production or by bcl 2 bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study aimed to carry out the comparative analysis of the molecular markers expression ( P 53 , Ki 67 , Her 2 / neu , Bcl 2 , Bax , ER , FasL and CD 95 ) at the cytologic and the correspondent histologic samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The behaviour of Bcl 2 , Bax and Bcl 10 in Darier ' s disease . ^^^ There is little information on the behaviour of Bcl 2 , Bax and Bcl 10 in DD . ^^^ OBJECTIVES : To investigate the dynamic control and the behaviour of Bax , Bcl 2 and Bcl 10 in DD . ^^^ CONCLUSIONS : The decrease or absence of Bcl 2 and Bcl 10 and the imbalance of Bax in the epithelial cells of affected DD skin is likely to be an important control point determined by the genetic mutation of SERCA 2 , which modifies the programme of the antiapoptotic proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 , Mcl 1 , Bcl xL / S , Bcl w , Bax , Bak , and Bad were shown to be expressed in both malignant and non neoplastic , normal plasma cells . ^^^ Quantitative analysis revealed that the malignant phenotype seemed to correlate with an elevated expression of Mcl 1 , a decreased expression of Bax and , to a lesser extent , an increased Bcl 2 / Bax expression ratio . ^^^ Furthermore , the anti apoptotic proteins Bcl 2 , Mcl 1 and Bcl xL were down regulated , while the expression of the pro apoptotic Bax protein was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We conclude , therefore , that IL 4 mediated apoptosis may participate in regulating catagen formation in the hair follicle , acting selectively on cultured FK and being independent of bcl 2 and bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro apoptotic Bcl 2 family members such as bax or bak are clearly required to initiate cytochrome c / apaf 1 / caspase 9 mediated cell death during oxygen deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also detected the expression of Bax , Bak , p 53 , Bcl 2 , Bcl 10 ( L ) , AIF and MRP 1 by Western blots . ^^^ Bcl 2 protein expression was significantly increased in p 50 , p 46 and p 33 transfected cells , while the expression of Bax , Bak , p 53 , Bcl 10 ( L ) and MRP 1 was essentially unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also evaluated by reverse transcriptase polymerase chain reaction ( RT PCR ) the expression of bax , bcl 2 , and caspas 3 , genes involved in apoptosis . ^^^ RT PCR analysis indicated in islets exposed to high FFA or glucose levels an increase of bax ( proapoptotic gene ) , a reduction of bcl 2 ( antiapoptotic gene ) , and a slight ( although not significant ) increase in caspase 3 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic members , such as Bcl 2 itself , are antagonistic to other family members , which prominently include Bax amongst a host of other proapoptotic proteins homologous to Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hepatocellular carcinoma and markers of apoptosis ( bcl 2 , bax , bcl 10 ) : prognostic significance . ^^^ Patients with tumors expressing promoters of apoptosis ( bax ) versus inhibitors of apoptosis ( bcl 2 , bcl 10 ) may have increased survival . ^^^ Seventy HCC were immunostained for bcl 2 , bax , and bcl 10 . ^^^ Staining frequency for bcl 2 , bax , and bcl 10 was 20 % , 66 % , and 60 % , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of KIT , CD 34 , vimentin , alpha smooth muscle actin ( SMA ) , s 100 , p 53 , ki 67 , bcl 2 and bax expression was performed on 20 surgically resected GIST . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transient overexpression of proliferating cell nuclear antigen was followed by increasing expression of p 53 , p 21 , Bax and caspases , whereas Bcl 2 and heat shock proteins were progressively repressed . ^^^ In these conditions , levels of proliferating cell nuclear antigen , Rb , Bcl 2 and heat shock proteins were persistently elevated , while expression of p 53 , p 21 , Bax and caspases gradually decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We examined cell population , proliferation , apoptosis , and immunohistochemical expression of apoptosis related proteins , Bcl 2 and Bax , in renal glomeruli of the Otsuka Long Evans Tokushima Fatty ( OLETF ) rat , an animal model of human type 2 diabetes . 10 , 30 , 50 , and 70 week old rats were used ( n = 5 8 ) . ^^^ Augmented expression of Bax , but not of Bcl 2 , was evident in glomeruli of OLETF rats during the late stage , which contributed to an increased Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study therefore , examines the expression of active and proactive caspase 3 , and the bax , bcl 2 and bcl 10 members of the bcl 2 family , to characterise the temporal profile of apoptosis in a model of traumatic brain injury in rats that produces significant diffuse axonal injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 1beta induced CFb apoptosis was associated with an increase in p 53 and Bax protein expression with no changes in Bcl 2 or Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
G ( 2 ) M arrest was associated with phosphorylation of Bcl 2 ( but not BAD , Bax , or Bcl XL ) : both of these end points were abrogated by treatment with a calcium chelator . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial damage and bcl 2 to bax balance play a central role in ischemia dependent apoptosis while angiotensin 2 and beta ( 1 ) adrenergic stimulation may be major causes of receptor mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Concomitant with the expression of p 53 and c Myc , decreased level of Bcl 2 and increased level of Bax protein were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent genetic studies with fibroblasts derived from mutant mouse embryos indicate that a class of the BCL 2 family proapoptotic proteins ( designated BH 123 or multidomain proteins ) such as BAX and BAK constitutes an essential component of the core apoptosis machinery in animal cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proliferation rate of the cells was determined by methyl thiazolyl tetrazolium ( MTT ) assay and positive expression of apoptosis associated proteins Bcl 2 and Bax were observed by means of flow cytometry . ^^^ RESULTS : It was observed that mifepristone at the dose of 1 . 25 micromol / L reversed the resistance of COC1 / DDP cells to cisplatin , and Bcl 2 protein expression was deceased from ( 23 . 8067+ / 0 . 4382 ) % to ( 19 . 3967+ / 0 . 6866 ) % ( P=0 . 000 ) while Bax protein expression increased from ( 12 . 75+ / 0 . 2524 ) % to ( 25 . 5967+ / 0 . 8834 ) % ( P=0 . 000 ) . ^^^ CONCLUSION : Mifepristone may act to enhance the sensitivity of COC1 / DDP cells to cisplatin , possibly through regulating Bcl 2 and Bax protein expressions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The degraded fragments of DNA in the cells were observed by agarose electrophoresis , the cell apoptosis rate determined by Annexin / V PI staining and p 53 , Bax and Bcl 2 proteins expression detected by flow cytometry . ^^^ RESULT : NADH not only inhibited the apoptosis induced by UVB irradiation , but also up regulated the expression of Bcl 2 protein and down regulated expressions of p 53 and Bax proteins ( P < 0 . 01 ) . ^^^ CONCLUSION : NADH significantly inhibits apoptosis induced by UVB irradiation possibly by the mechanism of up regulating Bcl 2 expression and down regulating p 53 and Bax expressions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cerebral cortical neuronal nuclei were isolated and nuclear proteins were separated electrophoretically and probed with specific antibodies against Bcl 2 or Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Survival probability depends on multiple biologic factors , including overexpression of Bcl 2 , p 53 , Bax , Bcl 10 ( L ) , MIB 1 , and apoptotic index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The resistance of this cell subset to starvation induced programmed cell death , lasting from 48 to 96 hours , is accompanied by a rise of mitochondrial adenosine triphosphate ( ATP ) , a high nicotinamide adenine dinucleotide ( NAD ) / reduced nicotinamide adenine dinucleotide ( NADH ) ratio , and by the up regulation of expression of the antiapoptotic proteins Bcl 2 and Bcl 10 , together with an increase in the cytoplasmic , inactive , form of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The different results obtained with these RMS cell lines does not appear to be related to expression of pax 3 FKHR , p 21 , Bax or Bcl 2 but may in part be due to differential regulation of p 53 target genes , such as MDM 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We hypothesized that apoptosis is a downstream event in erectile dysfunction , and pro apoptotic ( Bak and Bax ) and anti apoptotic ( Bcl 2 and Bcl 10 ) factors are involved in the etiology of aging erectile dysfunction . ^^^ Gene expression of pro apoptotic ( Bak and Bax ) and anti apoptotic ( Bcl 2 and Bcl 10 ) factors were then analyzed by reverse transcription polymerase chain reaction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax expression for hepatocellular apoptosis in a murine endotoxin shock model . ^^^ The aim of this study was to establish the relation of the time course of hepatic parenchymal cell apoptosis and necrosis in a murine endotoxin shock model and to characterize the role of the gene products of Bcl 2 and Bax in endotoxin mediated hepatocellular apoptosis . ^^^ Furthermore , Bcl 2 and Bax were analyzed by Western blotting . ^^^ Western blot analysis revealed that the apoptosis was accompanied by a decrease of Bcl 2 protein and a relative increase of Bax protein . ^^^ The apoptotic index decreased at 24 hours after the treatment synchronously with an increase in the ratio of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Semiquantitative RT PCR and Western blotting revealed activation of cell death related genes Bak and Bax of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The expression levels of mRNA for MDR 1 , MRP 1 , MRP 2 , p 53 , p 21 , Bax , and Bcl 2 were measured by real time quantitative polymerase chain reaction in HeLa and its MDR 1 overexpressing sublines . ^^^ The increase in Bax mRNA to Bcl 2 mRNA ratio after treatment with CDDP was suppressed in MDR 1 overexpressing cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , to the authors knowledge the clinicopathologic importance of the expression of apoptosis related genes ( bcl 2 , bax , and survivin ) in HCC remains unclear . ^^^ The expression levels of bcl 2 , bax , survivin , and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) mRNA were analyzed using real time reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ The bcl 2 / bax ratio and survivin / GAPDH ratio of the tumors were compared with clinicopathologic findings . ^^^ RESULTS : The bcl 2 / bax ratios of the tumors were not different from those of noncancerous liver tissue samples obtained from regions distant from the tumors and were not found to correlate with apoptosis or with clinical importance in HCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Unexpectedly , induction of apoptosis using IL 2 Bax was preceded by enhanced expression of newly synthesized Bax protein and suppression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 and bax genes during microcystin induced liver tumorigenesis ] . ^^^ METHODS : The two stage medium term tumorigenesis theory was applied to establish the animal model , and the effect of MC in liver tumor formation was evaluated by the Albert gamma GT methods , and then , the immunohistochemical technique and image analysis were used to study the expression of the bcl 2 and bax genes during tumorigenesis . ^^^ The intensity and areas of bax gene expression in the pure MC toxin group were significantly lower than those in the DEN control group ( P < 0 . 05 ) . ( 3 ) MC increased the expression of bcl 2 gene . ^^^ CONCLUSION : ( 1 ) MC can strongly promote liver tumorigenesis . ( 2 ) The changes of bcl 2 and bax gene expression possibly play an important role in the MC induced liver tumor formation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Genistein treatment resulted in the increase of Bax expression and its translocation into mitochondria , whereas expression levels of Bcl 2 remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Role of apoptosis , bcl 2 and bax protein expression in premature rupture of fetal membranes . ^^^ OBJECTIVE : To examine the degree of apoptosis in human fetal membranes associated with premature rupture of membranes ( PROM ) as compared with normal pregnancies and to evaluate the expression of proapoptotic bax and antiapoptotic bcl 2 gene products . ^^^ The membranes of both groups were then stained with bcl 2 and bax antibodies by using the standard steptavidin biotin immunoperoxidase method . ^^^ Both bax and bcl 2 expression was observed in 40 % of control cases and in 57 % and 50 % of cases with PROM , respectively , mostly in the chorionic trophoblastic cells . ^^^ The PROM and control groups showed no statistically significant difference in terms of bcl 2 and bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AT1R and AT2R protein expression ( Western immunoblot ) , CM apoptosis ( terminal deoxynucleotidyl transferase mediated 2 ' deoxyuridine 5 ' triphosphate nick end labelling assay and nuclear morphology ) and apoptotic markers ( Bax , Bcl 2 , caspase 3 , p 53 ) were assessed in LV tissue . ^^^ RESULTS : Compared with IR controls , CN improved peak systolic pressure , LV developed pressure and positive dp / dt , and increased AT2R ( not AT1R ) protein , but did not change the level of apoptosis or the expression of Bax , Bcl 2 , caspase 3 or p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of anti apoptotic proteins Bcl 2 and Bcl 10 ( L ) were increased , and the cellular levels of pro apoptotic proteins Bid and Bax were reduced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This cell death can be blocked by genetic deletion of Bax , a member of the pro apoptotic Bcl 2 family , cycloheximide an inhibitor of macromolecular synthesis or expression of dominant negative c jun . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interleukin 1 beta increases the BCL 2 / BAX ratio in Kaposi ' s sarcoma cells . ^^^ Using both an immunohistochemical and an immunoblot approach , we found that IL 1 increased the expression of Bcl 2 and decreased that of Bax , while having no effect on the expression of Bclx ( L ) , Fas and CD 40 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Amount of spontaneous apoptosis detected by Bax / Bcl 2 ratio predicts outcome in acute myeloid leukemia ( AML ) . ^^^ Bcl 2 and Bax oncoproteins were evaluated in 255 de novo AML patients ( pts ) by flow cytometry using an anti bcl 2 monoclonal antibody ( MoAb ) and an anti bax MoAb . ^^^ The results were expressed as an index ( bax / bcl 2 ) obtained by dividing bax mean fluorescence intensity ( MFI ) and bcl 2 MFI . ^^^ Lower bax / bcl 2 ratio was associated with French American British ( FAB ) M 0 M1 classes ( P = . 000 01 ) and CD 34 more than 20 % ( P < . 000 01 ) . ^^^ There were striking inverse correlations between CD 34 or CD 117 MFI and bax / bcl 2 values ( r = . 40 , P < . 000 001 and r = . 29 , P = . 000 002 ) , confirming that immaturity is consistent with this index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify the subcellular mechanisms of age associated dysregulation of apoptosis and the effects of CR , we analyzed the expression of genes promoting apoptosis ( p 53 , Fas receptor , Fas ligand , TNF receptor 1 , TNFalpha , Bax , TGF beta 1 ) and genes preventing apoptosis ( Bcl 2 and Bcl XL ) in the livers of 3 , 6 , 15 , and 24 month old male F 344 rats that were either fed ad libitum or subjected to a 30 % reduction in food intake ( CR ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Apoptosis ( by annexin 5 binding and the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling method ) , cellular reactive oxygen species ( ROS ) formation ( by fluorescent probe 2 ' 7 ' dichlorofluorescin diacetate and FACScan flow cytometry ) , and Bcl 2 and Bax proteins ( by immunostaining ) were studied in a human proximal tubular cell line ( HK 2 ) grown in a medium with physiologic ( 5 . 5 mM ) or high ( 30 mM ) glucose concentrations for 48 hours . ^^^ High glucose mediated apoptosis was associated with a decrease in the expression of Bcl 2 ( 87 % ) and a twofold increase in the expression of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The effects of TGF beta 1 on mesangial cell apoptosis , p 53 phosphorylation , Bax and Bcl 2 levels , caspase 9 activity , and cleavage of PARP were examined . ^^^ RESULTS : TGF beta 1 ( 2 ng / mL ) , which up regulates CK 2 activity , induces apoptosis in murine mesangial cells together with p 53 serine389 phosphorylation , up regulation of Bax , suppression of Bcl 2 , destabilization of mitochondrial permeability transition pores , stimulation of caspase 9 activity and activation of PARP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic effects of beta lapachone were associated with marked induction of p 53 phosphorylation and Bax protein without altering the expression of p 53 and Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Essential role for the BH 3 only protein Bim but redundant roles for Bax , Bcl 2 , and Bcl w in the control of granulocyte survival . ^^^ We have used gene targeted and transgenic mice to investigate the roles of the prosurvival molecules Bcl 2 and Bcl w and their proapoptotic relatives Bax and Bim in spontaneous and stress induced apoptosis of granulocytes from bone marrow or the peritoneum . ^^^ Bim deficiency , like Bcl 2 overexpression , rendered granulocytes resistant to cytokine withdrawal and cytotoxic drugs , but absence of Bax alone had no protective effect . ^^^ Granulocyte colony stimulating factor ( G CSF ) , a key survival factor for granulocytes , enhanced viability of cells lacking bcl 2 , bcl w , bax , or bim , indicating that none of these genes alone is the essential target of this cytokine ' s prosurvival function . ^^^ These results indicate that the BH 3 only protein Bim and possibly also Bmf are critical initiators of spontaneous and drug induced apoptosis of granulocytes , whereas Bcl 2 , Bcl w , and Bax act in a redundant manner in regulating granulocyte survival and death , respectively . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The release of cytochrome c is the central gate in turning on / off apoptosis , and is regulated by the interaction of proapoptotic proteins , including Bid , Bax and Bak , and antiapoptotic proteins including Bcl 2 and Bcl 10 ( L ) , and a specific class of inhibitors of apoptosis proteins ( IAPs ) including Akt , survivin , and heat shock proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The upregulation of proapoptic proteins , like Bax ( a member of the Bcl 2 family proteins ) , caspases and cytochrome c , with or without the downregulation of antiapoptic proteins , including Bcl 2 ( another member of the Bcl family ) , Akt and inhibitory apoptotic proteins ( IAPs ) , has been documented in different cardiac disease conditions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of bax , bcl 2 and bcl xL on regulating apoptosis in tissues of normal liver and hepatocellular carcinoma . ^^^ AIM : To investigate the expression of bax , bcl 2 and bcl xL mRNA in the tissues of normal liver and hepatocellular carcinoma ( HCC ) , and analyze the relationship between the expression of bax , bcl 2 and bcl xL mRNA and clinical parameters of HCC patients . ^^^ METHODS : The expression of bax , bcl 2 and bcl xL mRNA of normal liver and HCC was measured by Northern blot . ^^^ RESULTS : A very low mRNA level was indicated at bax , bcl 2 and bcl xL in the HCC tissues in contrast to the tissues of normal liver by Northern blot analysis . ^^^ The analyses of mRNA level revealed that HCC tissues exhibited a mean 7 . 6 fold decrease in bax , 4 . 2 fold in bcl 2 and 3 . 5 fold in bcl xL in comparison with normal control tissues , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An increase in low molecular weight DNA fragmentation indicative of apoptosis and an increase in the levels of Bax protein with no changes in Bcl 2 protein levels were evident in early antral follicles incubated for 24 h with Inh . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A decrease in the anti apoptotic protein , Mcl 1 , was detected in emodin treated HL 60 cells , whereas other Bcl 2 family proteins including Bax , Bcl 2 , Bcl XL , and Bad remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , RT PCR analysis indicated that MI / R significantly increased the ratio of Bax mRNA to Bcl 2 mRNA in aged rats compared to that of young rats ( 3 . 51 vs . 0 . 74 ) . ^^^ Furthermore , aged rats exhibit an increase in the ratio of Bax mRNA to Bcl 2 mRNA and cardiomyocyte apoptosis following MI / R , which may explain , at least in part , the enhanced myocardial dysfunction . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of c erbB 2 , p 53 , Bcl 2 , Bax , c myc and Ki 67 in apocrine metaplasia and apocrine change within sclerosing adenosis of the breast . ^^^ In 64 cases of APM and 18 cases of AA we examined for expression of c erbB 2 , p 53 , Bcl 2 , Bax , c myc and Ki 67 proteins using immunocytochemistry . c erbB 2 expression was seen in 55 . 6 % of AA cases and in 10 . 9 % of APM cases . p 53 expression was detected in 27 . 8 % of AA cases but only 1 . 6 % of APM cases . ^^^ All cases of AA and APM were negative for the anti apoptotic protein Bcl 2 , but all the APM and 33 . 3 % of AA cases showed cytoplasmic positivity for Bax , a pro apoptotic protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was studied by the terminal deoxynucleotidyl transferase ( TdT ) mediated deoxy UTP nick end labeling ( TUNEL ) method , and immunohistochemistry was used to detect p 53 , caspase 3 and 6 , the antiapoptotic proteins Bcl 2 and Bcl 10 , and the proapoptotic proteins Bax and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated its occurrence and time course in rat hindlimb skeletal muscles during the first 3 weeks of postnatal development , its morphological and biochemical features , and the concomitant expression of Bax , Bcl 2 , and Bcl 10 ( L ) . ^^^ Constitutive levels of Bax , Bcl 2 , and Bcl 10 ( L ) were detected by means of reverse transcriptase polymerase chain reaction ( RT PCR ) analysis at all ages examined , with a moderate increase around the period of maximal apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study was designed to investigate the expression of hypoxia inducible facter 1 alpha ( HIF 1 alpha ) and its relationship to bcl 2 , Bax , PCNA in lung cancer . ^^^ METHOD : Immunohistochemical streptavidin / peroxidase ( SP ) was used to examine the expression of HIF 1a , bcl 2 , Bax , and PCNA in 60 cases of lung cancer . ^^^ The positive rate of bcl 2 , Bax , and PCNA were 31 . 7 % ( 19 / 60 ) , 40 . 0 % ( 24 / 60 ) , 76 . 7 % ( 46 / 60 ) , respectively . ^^^ Inverse relationship was found between the expression of HIF 1 alpha and bcl 2 ; while the correlation of HIF 1 alpha and Bax was positive ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related proteins , poly ( ADP ribose ) polymersase ( PARP ) , bcl 2 and Bax protein levels were measured by Western blot . ^^^ Western blot analysis showed that PARP was proteolysized and Bax protein level was enhanced , whereas bcl 2 protein level was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Notably , expression of Bcl 2 prevented not only the oligomerization of Bax and Bak , but also the association between these two proteins in energy deprived cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An association of Bcl 2 phosphorylation and Bax localization with their functions after hyperthermia and paclitaxel treatment . ^^^ Therefore , we focused on the localization and phosphorylation of Bcl 2 and Bax , key mediators of the apoptotic pathway , after hyperthermia and paclitaxel treatment of PC 10 squamous cell carcinoma cells that excessively expressed Bcl 2 and Bax in the cytoplasm . ^^^ Paclitaxel treatment markedly induced qualitative changes in Bcl 2 , whereas hyperthermia did only quantitative changes in Bax . ^^^ The levels of Bax increased gradually with the duration of hyperthermia , whereas Bcl 2 levels slightly decreased . ^^^ Both treatments disturbed the heterodimerization of Bax with Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The correlation coefficients between Bcl 2 and MRP , Bcl 2 and Bax , p 53 and p 21 , as well as Ki 67 and PCNA were positive and significant , indicating their possible coregulated expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While Bcl 2 and Bax expression did not change , Bcl 10 ( L ) was down regulated and Bcl xs was up regulated after being exposed to flavopiridol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro and antiapoptotic proteins such as Bax and Bcl 2 complete in granulosa cells , in order to maintain or not the follicle alive . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , immunoblotting revealed that EGC treatment was correlated with a decrease in Bcl 2 and an increase in Bax level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that NF kappaB inhibition up regulated Bax ; down regulated the anti apoptotic proteins Bcl 2 , A 1 , and cIAP 2 ; and induced loss of the mitochondrial transmembrane potential and caspase independent , calpain dependent apoptosis in Rb cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 homology 3 ( BH 3 ) domain is present in most members of the Bcl 2 protein family and is required to confer the death inducing properties of pro apoptotic members , including Bax , Bak , Bad , and Bik , in cell based assay systems . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In 30 consecutive children with ALL treated with prednisone we determined changes in the expression of Bcl 2 , Bax and Bcl xl proteins in leukemic lymphoblasts and related these to clinical features and rate of prednisone induced apoptosis . ^^^ At diagnosis , we detected expression of Bcl 2 and Bcl xl protein in 28 samples , while Bax expression protein was detected in 21 of the 30 patients . ^^^ Prednisone treatment induced a decrease in Bcl 2 and Bcl xl levels in 17 and 16 of the 28 patients , respectively , while Bax protein increased in 14 of the 21 patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
And Bcl 2 over expression attenuates NPPA induced apoptosis by inhibiting caspase 3 activation , and subsequently inhibits calpain autolysis and Bax cleavage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To test this hypothesis , we treated endometrial cancer cells ( Ishikawa cell line ) with quercetin , and cell proliferation , expression of growth signal genes ( EGF , VEGF , and TGF alpha ) , cell cycle genes ( p 53 , p 21 , p 73 , and cyclin D 1 ) , and apoptosis related genes ( bcl 2 and bax ) were analyzed . ^^^ Other genes such as TGF alpha , p 53 , p 21 , p 73 , bcl 2 and bax were not significantly changed with quercetin treatment in Ishiwaka cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 members can either be anti ( Bcl 2 , Bcl 10 ( L ) , Bcl w ) or pro apoptotic ( Bax , Bak , Bid , Bad , Bcl 10 ( S ) ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of anti apoptotic proteins ( Bcl 2 , Bcl 10 ( L ) ) and pro apoptotic proteins ( Bax , Bcl 10 ( S ) , Bad , and Bak ) in response to cryo injury varied in this cell line panel . ^^^ The basal level of Bcl 2 / Bax protein ratio correlated inversely to the apoptotic rate . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CD 40 , CD 154 , Bax and Bcl 2 expression in Sjgren ' s syndrome salivary glands : a putative anti apoptotic role during its effector phases . ^^^ Our aim was therefore to further evaluate the expression of the pro and anti apoptotic Bax and Bcl 2 proteins . ^^^ We found the expression of Bcl 2 but rarely Bax in SS SG IMCs . ^^^ Bcl 2 in epithelial cells was sparse , while Bax expression occurred frequently and with no significant difference between the patient groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The morphologic observation and the expression of PCNA , bcl 2 , bax protein and bcl 2 mRNA were determined by microscope , immunohistochemistry , in situ hybridization and image analysis . ^^^ RESULTS : The expression of bax , bcl 2 protein and bcl 2 mRNA were higher than control group ( P < 0 . 01 ) . ^^^ CONCLUSIONS : HMBA could inhibit the MEC 1 cell proliferation by down regulation of PCNA expression , it could also induce apoptosis effectively that might be through the way of up regulation of bax and bcl 2 gene expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro death Bax increased , whereas anti death Bcl 2 and Bcl XL decreased , and apoptotic TUNEL positive cells were detected in the hippocampus of klotho mutant mice at the age of 7 wk . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Semi quantitative reverse transcription PCR was performed to detect the mRNA expression of bcl 2 c myc survivin bax s100A ( 2 ) TNFalpha TGFbeta ( 1 ) and IL 6 in the small cell lung cancer cell line NCI H 446 treated with antisense bcl 2 oligodeoxynucliotide . ^^^ RESULTS : bcl 2 AS PS ODN treatment could induce apoptosis , accompanied with 72 . 71 % up regulation of IL 6 and 65 . 90 % down regulation of TNFalpha , whereas little or no effect was seen on c myc survivin bax s100A ( 2 ) and TGFbeta ( 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 on the endoplasmic reticulum regulates Bax activity by binding to BH 3 only proteins . ^^^ Furthermore , we show that Bcl 2Cb5 can inhibit the activation of a constitutively mitochondrial mutant of Bax , indicating that an intermediate between Bcl 2 on the ER and Bax on the mitochondria must exist . ^^^ These data suggest that Bcl 2 indirectly protects mitochondrial membranes from Bax , via BH 3 only proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Predictive value of P 53 , BCL 2 , and BAX in advanced head and neck carcinoma . ^^^ Because the apoptotic process appears to be involved in the response to treatment of chemotherapy and radiotherapy , we investigated the prognostic value of the expression of three apoptosis associated genes ( p 53 , Bax , and Bcl 2 ) in tumor biopsies from patients with locally advanced head and neck carcinoma . ^^^ Using specific monoclonal antibodies , immunohistochemical staining for p 53 , Bax , and Bcl 2 was performed on tumor material from 43 patients before their scheduled adjuvant chemoradiotherapy . ^^^ Bax staining was positive in 8 cases ( 19 . 5 % ) , p 53 in 19 ( 47 . 5 % ) , and Bcl 2 in 4 patients ( 10 . 8 % ) . ^^^ These results suggest that p 53 , Bax , and Bcl 2 expression are not significant predictive factors of response to induction treatment in locally advanced head and neck carcinoma and that their routine use as prognostic markers can not be recommended . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We previously observed that the cyclin dependent kinase inhibitor p 16 ( INK4a ) and the proapoptotic Bcl 2 homolog Bax are positive prognostic factors and identify patients with good prognosis in esophageal squamous cell carcinoma ( SCC ) . ^^^ Protein expression profiles of cyclin D 1 , p 16 ( INK4a ) , Rb , p 21 ( CIP / WAF 1 ) , p 53 , Bax and Bcl 2 were analyzed by immunohistochemistry and compared to p 53 mutational status , as determined by SSCP PCR of exons 5 8 . ^^^ Loss of p 16 ( INK4a ) , Rb , p 21 ( CIP / WAF 1 ) or Bax and overexpression of cyclin D 1 were associated individually with shorter overall survival , while Bcl 2 expression and p 53 mutation were not of prognostic relevance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expressions of p 53 , bcl 2 , and bax were determined by Flow cytometry analysis . ^^^ Anti HPV 16 E6 ribozyme did significantly upregulate expression of p 53 , bax protein , and downregulate the expression of bcl 2 protein before and after radiotherapy ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using cultured prostate cancer ( PC ) cell lines , LN CaP and ALVA 31 , we studied the effects of 1alpha , 25 ( OH ) 2 Vitamin D 3 ( VD 3 ) on expression of several apoptosis regulating proteins including : ( a ) Bcl 2 family proteins ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , and Bak ) ; ( b ) the heat shock protein 70 binding protein BAG1L ; and ( c ) IAP family proteins ( XIAP , cIAP 1 , and cIAP 2 ) . ^^^ VD 3 induced decreases in levels of antiapoptotic proteins Bcl 2 , Bcl 10 ( L ) , and Mcl 1 , BAG1L , XIAP , cIAP 1 , and cIAP 2 ( without altering proapoptotic Bax and Bak ) in association with increases in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MCF 7 and ZR 75 breast cancer cells infected with an adenovirus constitutively expressing high levels of cyclin D 1 demonstrated widespread mitochondrial translocation of Bax and cytochrome c release that was approximately doubled after the addition of all trans retinoic acid ( RA ) or Bcl 2 antisense oligonucleotide . ^^^ Although constitutive Bcl 2 expression prevented Bax activation , it did not alter cyclin D 1 induced cell cycle arrest , illustrating the independence of these events . ^^^ Whereas high cellular levels of Bcl 2 prevent mitochondrial Bax translocation , lower levels can prevent apoptosis by inhibition of caspase activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical staining was performed to determine the tumor expression of p 53 protein , Bax , Bcl 2 , and survivin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the antiapoptotic proteins Bcl 2 and Mcl 1 was significantly decreased after LOX inhibitor treatment while that of the proapoptotic protein bax was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic effect of HA 14 1 , a Bcl 2 interacting small molecular compound , requires Bax translocation and is enhanced by PK 11195 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antisense oligonucleotides complementary to Bax transcripts reduce the susceptibility of B cell chronic lymphocytic leukaemia cells to apoptosis in a bcl 2 independent manner . ^^^ Furthermore , the reduction in Bcl 2 expression led to an increase in apoptotic cell death that was associated with an increase in Bax expression . ^^^ In this present study antisense oligonucleotides directed towards the Bax translation initiation sequence were employed to determine whether Bcl 2 and Bax protein expression were interdependent upon one another and whether Bcl 2 antisense induced apoptosis was mediated through a p 53 dependent cell death pathway . ^^^ Furthermore , these changes were independent of Bcl 2 expression suggesting that the previously observed increase in Bax expression following exposure of B CLL cells to Bcl 2 antisense oligonucleotides was probably caused by the induction of apoptosis rather than a rheostatic response to the reduction in Bcl 2 protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was shown that after treatment with 1 micro mol / L DNR , the fluorescence intensity index ( FI ) of both bcl 2 and c myc in HL 60 cells decreased , the FI of Bax , caspase 3 increased at 2 h , but decreased at 5 h , the FI of NF kappaB increased . ^^^ The mechanism was supposed by suppressing the expression of bcl 2 and c myc and activating the expression of Bax and caspase 3 , NF kappaB and ROS had the marked correlation with the apoptosis process , but the ceramide synthase was n ' t associated with it . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial outer membrane permeabilization by proapoptotic Bcl 2 family proteins , such as Bax , plays a crucial role in apoptosis induction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It has been reported that VCD induced follicle loss occurs via a cell death process involving elevated expression of Bax , a proapoptotic Bcl 2 family member , and increased caspase 3 like activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis regulating genes , bcl 2 and bax , and human telomerase reverse transcriptase messenger RNA expression in adrenal tumors : possible diagnostic and prognostic importance . ^^^ BACKGROUND : Apoptosis suppressing gene , bcl 2 , and apoptosis inducing gene , bax , are expressed in various tumors . ^^^ The purpose of this investigation was to determine whether bcl 2 or bax gene expression differs between benign and malignant adrenal tumors , and whether hTERT gene expression correlates with the prognosis of patients with adrenal cancer . ^^^ METHODS : Expression of bcl 2 and bax from 52 adrenal surgical specimens was analyzed by reverse transcription polymerase chain reaction . ^^^ RESULTS : All normal adrenal tissues ( n = 7 ) and benign adrenal tumors ( n = 41 ) expressed bcl 2 and bax , whereas all adrenocortical cancers ( n = 4 ) expressed bcl 2 but not bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Topographic association of gastric epithelial expression of Ki 67 , Bax , and Bcl 2 with antralization in the gastric incisura , body , and fundus . ^^^ The aim of this study was to determine the topographic associations of Ki 67 ( a protein expressed in proliferative cells ) , Bax ( a pro apoptotic protein ) , and Bcl 2 ( an antiapoptotic protein ) expression with antralization . ^^^ METHODS : In each of 104 patients , eight biopsy specimens were taken from the gastric antrum , incisura , body , and fundus for the determination of H . pylori infection , histological changes , and epithelial expression of Ki 67 , Bax , and Bcl 2 . ^^^ At the incisura , Ki 67 LI was greater ( 47 vs 32 , p < 0 . 001 ) , Bax LI was lower ( 22 vs 30 , p < 0 . 05 ) , and prevalence of Bcl 2 overexpression was higher ( 44 % vs 18 % , p < 0 . 001 ) in the presence ( vs absence ) of antralization . ^^^ CONCLUSIONS : Antralization at the incisura is topographically associated with increased cell proliferation , reduced Bax expression , and Bcl 2 overexpression , which implies that antralization may be an important histological marker for future cancer risk . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although an activating conformational change in Bax also was evident in the apoptotic cells , enforced overexpression of Bcl 2 was insufficient to prevent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Doxorubicin did not affect steady state levels of Bax , Bcl 2 and Bcl 10 ( L ) in the majority of the prostate cancer cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exposure to DX after radiation in PC 3 / vector significantly abrogated radiation induced Bcl 2 upregulation , with a concomitant induction of bax protein . ^^^ In PC 3 / Bcl 2 transfectants , DX exposure after IR caused an induction of bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We find that Bax , a proapoptotic member of the Bcl 2 family , translocates to discrete foci on mitochondria during the initial stages of apoptosis , which subsequently become mitochondrial scission sites . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A dose related increase in the expression of Fas antigen , Bax and Caspase 3 , as well as a decrease in the expression of Bcl 2 , were determined for the adriamycin treated cardiomyocytes using Northern blot analysis , reverse transcriptase polymerase chain reaction ( RT PCR ) and ribonuclease protection assay . ^^^ RT PCR also revealed increased Fas antigen expression , decreased Bcl 2 expression , and no change in Bax expression for the adriamycin treated human umbilical vein cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The release of cytochrome c can be influenced by different Bcl 2 family member proteins , including Bax , Bid , Bcl 2 , and Bcl 10 ( L ) . ^^^ Bax and Bid potentiate cytochrome c release , whereas Bcl 2 and Bcl 10 ( L ) antagonize this event . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DES induced the expression of 12 genes ( bad , bax , bcl 10 , caspase 1 , p 53 , cyclin D 3 , GADD 45 , p 21 , p 15 , p 27 , p 57 and Skp 1 ) and down regulated the expression of eight genes ( bcl 2 , caspase 2 , caspase 7 , caspase 8 , E 124 , iNOS , mdm 2 and NFkappab 1 ) at twofold or greater levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of fas , p 53 , bax , and bcl 2 protein were determined by flow cytometric analysis ( FCM ) . ^^^ RESULTS : Antisense alpha 1 , 4Gal T oligonucleotide signifantly inhibited cell growth ( P < 0 . 01 ) , induced the apoptosis ( P < 0 . 05 ) , downregulated expression of bcl 2 protein ( P < 0 . 01 ) and upregulated expressions of fas and bax protein ( P < 0 . 01 ) , but did not influence p 53 expression in glioma cell line SWO 38 . ^^^ CONCLUSIONS : Antisense alpha 1 , 4Gal T oligonucleotide can significantly inhibit proliferation and induce apoptosis in human gliomas cell line SWO 38 , which maybe due to the interactions of fas , bax , and bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was used to examine the expression of Bax , Bcl 2 after treatment of triptolide and celastrol . ^^^ They both increased expression of Bax and decreased expression of Bcl 2 in the SHG 44 cells . ^^^ CONCLUSION : Triptolide and celastrol inhibit the proliferation of glioma cells in vitro , which was associated with promoting the expression of Bax and inhibiting the expression of Bcl 2 and accelerating cell apotosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This is not likely to be due to altered apoptotic signaling since the expression of Bax and Bcl 2 was unchanged and the induction of Fas / CD95 / APO 1 receptor and caspase 8 was the same in p 53 ( / ) and p 53 wt cells on treatment with doxorubicin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At 2 , 4 , 6 , 8 and 12 weeks after the injection , the apoptotic fibroblast count in the scar was determined by TUNEL method , and the expressions of apoptosis related modulation proteins p 53 , bcl 2 , bax were detected by immunohistochemistry method . ^^^ Furthermore , the bax expression was increased and that of p 53 and bcl 2 was decreased significantly in M group . ^^^ CONCLUSION : Matrine might obviously enhance the fibroblast apoptosis in rabbit ear hypertrophic scar , and up regulate the expression of apoptosis related modulation protein bax and down regulate the expression of p 53 and Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cell growth inhibition of cells correlated well with increase in Bax : Bcl 2 ratio and poly ( ADP ribose ) polymerase ( PARP ) cleavage . ^^^ Western blot analysis of cells isolated from s . c . tumors showed no induction of the Bax protein and a very low Bax : Bcl 2 ratio of approximately 0 . 3 in correlation with minimum PARP cleavage . ^^^ However , there was no change of apoptosis related molecules such as PARP , Bax , and Bcl 2 or reduction in tumor weight in association with Topo IIbeta inhibition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In MB 468 cells with overexpression of Bcl 2 ( 468 / Bcl 2 ) , FP induced Bax conformational change and apoptosis were inhibited , whereas the FP mediated decline in the levels of IAP proteins , Mcl 11 and Bcl 10 ( L ) remained unaltered . ^^^ Taken together , these findings suggest that the superior sequence dependent anti breast cancer activity of Epo B followed by FP may be due to FP induced Bax conformational change and down regulation of the antiapoptotic IAP , Bcl 10 ( L ) , and Mcl 1 proteins , but this treatment may not overcome the resistance to apoptosis of breast cancer cells conferred by overexpression of Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The expression of apoptosis associated proteins ( bcl 2 , bcl xL , bax , bcl xs ) , the activity of caspase 3 and cleavage of poly ADP ribose polymerase ( PARP ) were determined with Western blot analysis in the cisplatin resistant cell ( COC1 / DDP ) and cisplatin sensitive human ovarian cancer cell ( COC 1 ) . ^^^ RESULTS : The expression of bcl 2 and bcl XL in COC1 / DDP cell was significantly higher than that in COC 1 cell , whereas the expression of bax showed no change in COC1 / DDP and COC 1 . ^^^ CONCLUSIONS : Cisplatin resistance in human ovarian cancer cell lines may associated with the overexpression of anti apoptotic protein bcl 2 and downregulation of caspase 3 activity , but not associated with the expression of bax and bcl xs . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Flow cytometry and a panel of antibodies were used to analyze the expression of MDR proteins ( P gp , MRP , LRP , BCRP , GST pi ) and apoptosis modulating proteins ( bcl 2 , bcl 10 , bax , bad ) in MDR cell line HL 60 / VCR and drug sensitive cell line HL 60 . ^^^ The levels of apoptosis modulating proteins ( bcl 2 , bcl 10 , bad ) were ( 2 . 48 , 1 . 25 , 1 . 08 fold ) higher in HL 60 / VCR than in HL 60 , while the pro apoptosis protein bax contrarily decreased in HL 60 / VCR . ^^^ CONCLUSION : Various MDR mechanisms were involved in multi drug resistance HL 60 / VCR cell line , which including increasing expression of drug resistance protein ( P gp , MRP , BCRP , and GST pi ) ; the apoptosis modulating proteins ( bcl 2 , bcl 10 , bax , and bad ) might take part in the mechanism of drug resistance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , tea polyphenols and tea pigments induced the expression of p21WAF1 protein , inhibited the expression of Bcl 2 protein and induced the expression of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Electrophoresis and Western blotting were used to analyze DNA fragmentation and expression of DNA fragmentation factor ( DFF 45 ) , Bcl 2 , Bcl xL and Bax genes respectively . ^^^ The anti apoptotic genes Bcl 2 and Bcl xL were down regulated and the pro apoptotic gene Bax was expressed at higher levels compared with the euthyroid state . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax proteins in liver tissues of chronic viral hepatitis ] . ^^^ The results showed that Bcl 2 and Bax were mainly colocated in cytoplasm of the mononuclear cells ( MN ) in the areas of focal necrosis , bridging necrosis and piecemeal necrosis ( PN ) . ^^^ Some hepatocytes surrounding these areas expressed Bcl 2 and Bax or expressed Bax only . ^^^ In the severe viral hepatitis , survival hepatocytes also expressed Bcl 2 and Bax . ^^^ It is suggested that the expression of Bcl 2 and Bax in the liver tissues correlated with the apoptosis of hepatocytes in CH and influenced the progress of severe viral hepatitis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also show that signals transmitted by tyrosine 1062 mediate proapoptotic events like Bcl 2 down regulation and Bax upregulation , and that adoptive overexpression of Bcl 2 overcomes RET / PTC induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The translocation of Bax alpha , a pro apoptotic member of the BCL 2 family from the cytosol to mitochondria , is a central event of the apoptotic program . ^^^ Taken together , our results suggest that Bax is targeted to mitochondria by its NT and thus through a pathway that is unique for a member of the BCL 2 family . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment with G 3139 led to a sequence specific reduction of Bcl 2 protein levels within 4 days of exposure in 10 out of 11 clinical samples from patients with chemosensitive and multidrug resistant disease , without significant reduction of alpha Actin , Bax , Bcl XL , or Mcl 1 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Deferoxamine mesylate induced a small increase in the endogenous expression of both the bcl 2 and bax genes in the MCF 7 cells and this can be prevented by ferric chloride . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of p 53 , p21WAF 1 and Bax increased , in contrast to the gradually decreasing expression of Bcl 2 , as observed by immunoblotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We detected apoptosis with the in situ terminal deoxynucleotidyl transferase assay and flow cytometry , and measured the expression of several apoptotic regulatory proteins ( Bcl 2 , Bax , Bclx , NF kappa B ) with Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results were as follows : Ad RA 538 and Ad ASc myc could strongly inhibit cell growth and induce apoptosis of SGC 7901 cells in vitro and in vivo , and could down regulate expression of c myc , bcl 2 and cyclinD 1 gene , up regulate expression of bax gene . ^^^ They relate to c myc , bcl 2 , cyclinD 1 and bax gene closely and play a key role on biologic effects in gastric cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apo2L / TRAIL induced the expression of pro apoptotic proteins , Bad and Bax ; downregulated the anti apoptotic proteins , Bcl 2 and Bcl xL ; and activated caspases 3 , 7 , 8 , 9 and 10 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax expression in rat cerebral ischemic preconditioning ] . ^^^ OBJECTIVE : To study the expression of apoptosis regulated genes bcl 2 and bax nocoprotein expression and its significance in rat cerebral ischemic preconditioning . ^^^ METHODS : With immunohistochemical assay , bcl 2 and bax expression were studied in rat brains which were divided into four groups : the sham operation control group , the cerebral ischemic group and the cerebral ischemic preconditioning group . ^^^ CONCLUSIONS : The expression of bcl 2 and bax oncoprotein after sublethal ischemic preconditioning might be associated with the preconditioning protection against neuronal damage following subsequent lethal ischemia . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Study on histology and the expression of Bax and Bcl 2 after cerebral contusion in rats ] . ^^^ To set up the experimental model of focal cerebral contusions in rats , and to study the histology and the expression of Bax and Bcl 2 after cerebral catusions . ^^^ The expression of Bax and Bcl 2 increased after injury . ^^^ There existed a relationship between increased Bax / Bcl 2 and different intervals after brain injury . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we investigated whether the neuroprotection previously seen with dexmedetomidine or S ( + ) ketamine involves regulation of proapoptotic ( Bax and p 53 ) and antiapoptotic ( Bcl 2 and Mdm 2 ) proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , anti apoptotic protein Bcl 2 was down regulated resulting in decrease in Bcl 2 / Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The percentage of apoptosis by flow cytometry and Fas , Bax , and Bcl 2 protein expression by immunoblotting were evaluated at entry in patients and control subjects and repeated after 12 months in group 3 . ^^^ In group 3 , apoptosis and the Bax / Bcl 2 ratio decreased at 1 year ( P < 0 . 0001 , P < 0 . 05 respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism of protection afforded by muscarinic receptor activation from camptothecin induced apoptotic signaling involved blockade of mitochondrial cytochrome c release associated with a bolstering of mitochondrial bcl 2 levels and blockade of the translocation of Bax to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Study on apoptosis and expression of P 53 , bcl 2 , Bax in cardiac myocytys of congestive heart failure induced by ventricular pacing . ^^^ The apoptosis and the expression of p 53 , bcl 2 and Bax in myocytes of chronic rapid ventricular pacing induced congestive heart failure ( CHF ) in rabbits were investigated . ^^^ By using TUNEL technique the apoptosis in the myocytes in the rabbit model was studied and the expression of p 53 , bcl 2 and Bax in myocytes was detected by using immunohistochemical method . ^^^ Myocytes positive for p 53 immunoreactivity ( 18 . 86 + / 8 . 48 vs 5 . 06 + / 0 . 87 , P < 0 . 01 ) and positive for Bax immunoreactivity ( 7 . 15 + / 1 . 91 vs 0 . 43 + / 0 . 09 , P < 0 . 01 ) were increased in P group as compared with those in C group , while the myocytes positive for bcl 2 immunoreactivity ( 7 . 08 + / 1 . 05 vs 14 . 97 + / 4 . 47 , P < 0 . 01 ) and the ratio of bcl 2 / Bax were decreased in P group as compared with those in C group . ^^^ The expression of p 53 and Bax was increased , while the expression of bcl 2 was inhibited . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and Bax genes in facial neurons and their regulation of neuron apoptosis following facial neurotmesis in rats ] . ^^^ OBJECTIVE : The aim of this study is to investigate the relationship between the expression changes of bcl 2 and Bax genes and their functions in facial neuronal apoptosis after amputating the facial nerve . ^^^ The changes of expression of bcl 2 and Bax genes in the facial neurons and facial neuronal apoptosis were observed after the facial nerve amputation using the methods of ABC immunohistochemical staining and TUNEL . ^^^ RESULTS : The expression of bcl 2 and Bax genes and the apoptosis of facial neurons were significantly higher in the experimental group than those of the control group from the 3rd to 21st day after amputation , but the bcl 2 / Bax significantly decreased compared with that of the control group . ^^^ CONCLUSION : This indicated that bcl 2 and Bax might play an important regulation role in the process of facial neurons apoptosis following facial nerve amputation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increased apoptosis was accompanied by decreased antiapoptotic bcl 2 mRNA expression among bcl 2 related genes ( bcl 2 , bax , bak , mcl 1 , and bcl 10 ( L / S ) ) , and the effect was also more prominent in the cagA ( + ) strains . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax modulate adenine nucleotide translocase activity . ^^^ Conversely , Bax , which displaces Bcl 2 from ANT in apoptotic cells , inhibits ADP / ATP exchange through a direct action on ANT . ^^^ These data are compatible with a model of mitochondrial apoptosis regulation in which ANT interacts with either Bax or Bcl 2 , which both influence ANT function in opposing manners . ^^^ Bcl 2 would maintain the translocase activity at high levels , whereas Bax would inhibit the translocase function of ANT . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of vasostomy on expression of Bcl 2 and Bax gene in rat spermatogenic cells ] . ^^^ The objective of this study was to explore the change of Bcl 2 and Bax gene expression in rat spermatogneic cells after vasostomy . ^^^ A rat model with vasoligation and vasostomy was set up and then used for detecting the protein expression of Bcl 2 and Bax gene at 4 , 8 , 12 , 16 weeks after vasostomy by means of immunohistochemistry . ^^^ In conclusion , the change of Bcl 2 protein expression observed in this study is indicative of the anti apoptosis function of Bcl 2 gene in both the status of being vasoligated and vasostomized , whereas the consistent Bax protein expression before and often the repatency of the vas suggests further researches into its effect on apoptosis of spermatogenic cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent work shows that two pro apoptotic Bcl 2 family proteins , Bax and Bid , as well as the mitochondrion specific lipid cardiolipin may cooperate in chemically defined liposomes to generate a protein permeable conduit , relaunching the debate on the identity of the pore responsible for mitochondrial membrane permeabilization during apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of PIC BE on the expression of mdr 1 , bcl 2 and bax genes and their protein products ( P gp , Bcl 2 and Bax ) was observed respectively in a multidrug resistance ( MDR ) cell variant K562 / ADM . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis was performed to determine BRCA 1 and the apoptosis related proteins bcl 2 , Bax and p 53 in paraffin embedded tissues of 156 sporadic invasive ductal carcinomas . ^^^ BRCA 1 expression was positively correlated with Bcl 2 expression ( p = 0 . 0008 ) , but no relationship between BRCA 1 expression and Bax or p 53 expression could be established . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The rates of apoptosis and alterations of Bcl 2 and Bax expression were analyzed by flow cytometry . ^^^ Induction of apoptosis was accompanied by the down regulation of Bcl 2 expression , but there was little alteration of Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Characterization of Bax and Bcl 2 in apoptosis after experimental traumatic brain injury in the rat . ^^^ This study was undertaken to fulfill the need for additional data on the dynamics of Bax and Bcl 2 expression in conjunction to the cell death that ensues following experimental brain contusion . ^^^ Expression of Bax and Bcl 2 were analyzed by immunohistochemistry and in situ hybridization . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Acinar cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling ( TUNEL ) , caspase 3 activity , and Bax / Bcl 2 expression . ^^^ In addition , the exocrine pancreas of Cx 32 deficient mice showed a decreased number of TUNEL positive acinar cells and decreased caspase 3 activity but no change in Bax or Bcl 2 pancreatic expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis triggered by P . urinaria extract in Lewis lung carcinoma cells was associated with the down regulation of Bcl 2 gene expression , but not with p 53 , p 21 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results demonstrated a tendency for stronger and more frequent expressions of c myc , Bak and Bax despite a rather weaker expression of Bcl 2 in cancer tissues from the elderly compared with those from the younger patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nonetheless , there were no significant alterations in the level of Bcl 2 , Bcl 10 ( L ) , Bax and Bak by the proteasome inhibitor , nor any evidence of cytochrome ( cyt ) c release into cytosol from dying cells , suggesting that cyt c is not involved . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of known effector molecules mediating mitochondrial signaling , such as Bax and Bid , and the antagonistic molecule Bcl 2 did not influence sensitivity of B CLL cells to TC A . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of apoptosis related proteins , Bcl 2 , Bax , and c Myc , was measured by an immunohistochemical method , and c Myc mRNA expression was determined by the reverse transcription polymerase chain reaction . ^^^ The expression of the Bax and c Myc proteins and of c Myc mRNA was increased , whereas Bcl 2 expression was decreased after co culture for 48 hours . ^^^ CONCLUSIONS : Helicobacter pylori induced apoptosis in gastric epithelial cells is mediated by altered expression of the products of the Bcl 2 , Bax , and c Myc genes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Ki 67 , p 53 , Bax and Bcl 2 proteins was determined by immunohistochemical staining . ^^^ After chemotherapy , the expression of Bax protein in responders was more frequent and Bcl 2 protein expression was less frequent than in non responders . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alteration of the expression of Bcl 2 , Bcl 10 , Bax , Fas , and Fas ligand in the involved skin of psoriasis vulgaris following topical anthralin therapy . ^^^ In normal skin , keratinocytes expressed low to absent levels of Bcl 10 and Bax , while Bcl 2 was detected only in melanocytes in basal layers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate a cause and effect relationship between these two phenomena , we evaluated the effects of RU 486 treatment on the expression of two apoptosis related genes , bax and bcl 2 , known to be regulated through NF kappa B binding on their promoter . ^^^ RT PCR demonstrated that RU 486 significantly induced bax mRNA levels , while suppressing mRNA of bcl 2 . ^^^ This study demonstrates that the inhibition of growth and apoptosis of human endometrial cells by RU 486 involves stimulation of NF kappa B binding with subsequent modulation of apoptosis regulatory genes bax and bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein expression of apoptosis associated gene ( Bcl 2 , Bax , c Jun ) were examined by using immunocytochemical SABC method . ^^^ The expression of Bcl 2 was lowered , while Bax and c Jun protein were increased . ^^^ TXR could inhibit the apoptotic process via regulating the expression of bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fifty excisional biopsy samples were studied by immunohistochemical technique for the differential expressions of bcl 2 , bax , bcl 10 and alpha smooth muscle actin . ^^^ Bcl 2 , bcl 10 and bax were expressed in 34 ( 68 % ) , 38 ( 76 % ) and 41 ( 82 % ) specimens , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Epoxomicin also increased the expression of Bax and decreased that of Bcl 2 , which may cause mitochondrial dysfunction and release of free radicals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The neutrophils showed signs of apoptosis with increased caspase activity , condensed nuclei , and perinuclear clustering of mitochondria to which the proapoptotic Bcl 2 member Bax had translocated already . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , expression of the apoptotic regulators bcl 2 and bax was analyzed . ^^^ CD 40 expression correlated with bax but not with bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We investigated proteins involved in regulation of apoptosis ( p 53 , BAX , BCL 2 , and BCL 10 ( L ) ) , cell cycle control [ p 21 and retinoblastoma protein ( RB ) ] , and drug export and inactivation [ P glycoprotein , multidrug resistance associated protein ( MRP ) 1 , MRP 2 , breast cancer resistance protein , lung resistance protein , metallothionein , and glutathione S transferase pi ] immunohistochemically in samples of unselected GCT patients ( n = 20 ) , patients with advanced metastatic disease in continuous remission after first line chemotherapy ( n = 12 ) , and chemotherapy refractory patients ( n = 24 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Phytosphingosine induced mitochondrial translocation of Bax from the cytosol without changes in the protein levels of Bcl 2 , Bcl xL , and Bax . ^^^ In addition , Bcl 2 / Bax interaction was diminished after addition of phytosphingosine . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell cycle perturbations and apoptosis were evaluated by flow cytometry , mitotic index by microscopic analysis , bax , bcl 2 , and p 53 by immunohistochemistry , and cyclin B expression by Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bak expression was compared with our previous investigated apoptosis related parameters such as apoptotic index ( AI ) , Bax and Bcl 2 expression , and p 53 accumulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA , Bcl 2 and Bax genes in mice bearing H 22 solid hepatic carcinoma . ^^^ Immunohistochemical staining showed that the number of Bcl 2 protein positive cells were decreased , but the number of Bax protein positive cells were increased . ^^^ The mechanism of anticancer effect of artesunate may be related to down regulation of the expression of PCNA and Bcl 2 genes and up regulation of the expression of Bax gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot was made to analyse protein levels of anti apoptotic Bcl 2 and proapoptotic Bax . ^^^ L 1 tacrine significantly increased the expression of Bcl 2 protein level and delayed the staurosporine induced increase of Bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of apoptosis related genes BCL 2 , BAX , BCL2L1 , BCL2A1 , MCL 1 , DAPK 1 and MYC was studied by quantitative real time polymerase chain reaction on total RNA samples from patients with acute lymphoblastic leukaemia ( ALL , n = 16 ) , acute myeloid leukaemia ( AML , n = 27 ) , chronic myeloid leukaemia ( CML , n = 12 ) , mantle cell lymphoma ( MCL , n = 19 ) and chronic lymphoid leukaemia ( CLL , n = 32 ) . ^^^ BCL 2 , BAX , BCL2A1 , MCL 1 , DAPK 1 and MYC were overexpressed in all patient groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , bax and bcl 10 protein expression were detected by standard avidin biotin peroxidase method . ^^^ Bcl 2 , bax and bcl 10 expression were detected in 84 % , 80 % , and 76 % , respectively , of normal squamous mucosa . ^^^ Pre therapeutic bcl 2 , bax and bcl 10 protein expression ( 27 % , 75 % , and 87 . 5 % , respectively ) were not associated with tumor response or resistance to therapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax expression in advanced non small cell lung cancer : lack of correlation with chemotherapy response or survival in patients treated with docetaxel plus vinorelbine . ^^^ Surgical series of non small cell lung cancer ( NSCLC ) pathologic samples have shown that the expression of the proteins bcl 2 and bax , which regulate cell death , may have prognostic implications . ^^^ In order to determine the rate of bcl 2 and bax expression in advanced NSCLC and assess the impact on chemotherapy response and patient survival , we performed immunohistochemistry on biopsy samples from patients enrolled in a phase I / II trial of vinorelbine plus docetaxel . ^^^ The goal of this study was to determine the feasibility of this analysis , and to observe any differences in response rate or survival based on bcl 2 or bax staining results . ^^^ Bcl 2 staining was positive in 5 / 31 samples ( 16 % , 95 % CI 3 29 % ) and bax was positive in 19 / 28 samples ( 68 % , 95 % CI 51 85 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bcl 2 , fas and Fas L antigen expression in human seminoma : correlation with the apoptotic index . ^^^ Apoptosis plays a crucial role in the regulation of spermatogenesis in male germ cells and is , at least in part , modulated by Bcl 2 , Bax , and the Fas pathway . ^^^ The expression of Bax , Bcl 2 , Fas and Fas ligand ( Fas L ) in human seminoma was evaluated and correlated with the apoptotic index . ^^^ Twenty nine classical seminomas were examined by immunohistochemistry and Western blotting using antibodies against Bax , Bcl 2 , Fas and Fas L . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Expressions of T cell apoptosis promoting and inhibiting genes , including Fas , FasL , Bcl 2 , Bax , TNFR 1 and TNFR 2 , as well as activity of cysteine proteinase in cascade connection , such as Caspase 8 and Caspase 3 were determined by TUNEL labeled flow cytometry and fluorescence real time quantitative RT PCR technique . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Flow cytometric assay was performed to quantify the apoptotic cells and detect the positive percentage of Bcl 2 and Bax proteins in PC 12 cells . ^^^ The expression of bcl 2 and bax mRNA was identified by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Simultaneously , the expression of Bcl 2 protein and mRNA were increased , and the Bax protein and mRNA were decreased . ^^^ CONCLUSION : Rg 1 protects against dopamine induced apoptosis in PC 12 cells and this effect might be attributed to its regulation of the ratio of Bcl 2 to Bax and its inhibition of the activation of CPP32 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since selegiline has an antioxidant antiapoptotic effect , we proposed to determine whether selegiline attenuates cardiac oxidative stress and myocyte apoptosis in chronic heart failure by modulating Bcl 2 and Bax protein expression , and whether the effects are associated with the improvement of cardiac function . ^^^ After 8 weeks of treatment , animals were studied for arterial norepinephrine and left ventricular systolic function ( fractional shortening and dP / dt ) , and were then sacrificed for measuring the stable oxidative product of myocardial mitochondrial DNA ( mtDNA ) 8 oxo 7 , 8 dihydro 2 ' deoxyguanosine ( 8 oxo dG ) , myocyte apoptosis by monoclonal antibody to single stranded DNA , and Bcl 2 and Bax protein expression by Western blot and immunohistochemistry . ^^^ Rapid cardiac pacing increased plasma norepinephrine , cardiac oxidative stress and myocyte apoptosis , reduced Bcl 2 and the Bcl 2 to Bax ratio . ^^^ Selegiline treatment in chronic heart failure animals reduced plasma norepinephrine , cardiac oxidative stress and myocyte apoptosis , prevented the changes of Bcl 2 and Bcl 2 to Bax ratio , and improved left ventricular fractional shortening and dP / dt . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sequential analyses were made following ethanol exposure on these two postnatal days , with assessments of NTFs nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and neurotrophin 4 ( NT 4 ) ; apoptosis related proteins Bcl 2 , Bcl xl , Bax , Akt and c jun N terminal kinase ( JNK ) ; antioxidants superoxide dismutase , glutathione reductase and catalase ; and ROS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was used to examine expression of BCL 2 , BAX , P 53 , CD 40 and CD40L in archival tissues of patients included in various treatment trials for transitional cell carcinoma ( TCC ) of the bladder . ^^^ Median survivals for the various markers were as follows : BAX positive patients 110 m vs BAX negative patients 18 m ( P=0 . 0002 ) ; CD40L positive patients 95 m vs CD40L negative patients 45 m ( P=0 . 04 ) ; BCL 2 positive patients 44 m and BCL 2 negative patients 74 m , ( P=0 . 64 ) ; CD 40 positive patients 110 m and CD 40 negative patients 45 m ( P=0 . 12 ) ; and P 53 positive patients 80 m and P 53 negative patients 45 m ( P=0 . 58 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of HSP 70 and messenger RNA ( mRNA ) , apoptosis , and apoptosis associated genes such as Bcl 2 and Bax were studied . ^^^ In livers subjected to longer duration of warm ischemia , necrosis and apoptosis were evident and Bcl 2 mRNA expression and Bcl 2 / Bax protein ratio were markedly diminished . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of apoptosis associated gene fas , bax , p 53 and p 21 were up regulated ; the expression of bcl 2 was down regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis levels were quantified by use of a fluorescent terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling ( TUNEL ) stain , and Bcl 2 and Bax were measured by semiquantitative immunohistochemical assays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell proliferation , apoptosis , and expression of Bcl 2 and Bax in non lactating human breast epithelium in relation to the menstrual cycle and reproductive history . ^^^ Cell proliferation , apoptosis , and the expression of Bcl 2 and Bax were investigated in breast tissue of healthy premenopausal women in order to study the effect of the menstrual cycle and reproductive history on the cell turnover in the non lactating mammary gland epithelium . ^^^ Immunohistochemistry was used to detect the proliferation associated antigen Ki 67 , as well as Bcl 2 and Bax . ^^^ Staining patterns for Bax and Bcl 2 showed characteristic changes due to the menstrual cycle with a maximum of immunoreactivity for Bcl 2 in the follicular phase and for Bax in the luteal phase . ^^^ However , there was no statistically significant association between Bcl 2 / Bax immunoreactivity and menstrual cycle or reproductive parameters . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of IFN a is not accompanied by a modulation of CD 95 , CD95L , p 53 , p 21 ( WAF 1 ) , p 27 , bcl 2 , bcl xL , bax , NFkappaB , or IkappaB gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mouse uterine epithelial apoptosis is associated with expression of mitochondrial voltage dependent anion channels , release of cytochrome C from mitochondria , and the ratio of Bax to Bcl 2 or Bcl 10 . ^^^ In addition , the apoptotic index showed good correlation with the release of cytochrome c from mitochondria , activation of caspase 3 , which was immunohistochemically detected only in the epithelium , and the mRNA and protein ratios of Bax : Bcl 2 and Bax : Bcl 10 in the uterus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Enhanced expression of Bax : Bcl 2 ratio was detected in placenta and decidua in the death group compared with that in the survival group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 and Bax was examined using reverse transcription polymerase chain reaction and Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As the factors regulating cell death in benign schwannomas are not well understood , we investigated the immunoexpression of the Fas Fas L system , as well as that of the anti apoptotic factor Bcl 2 and the pro apoptotic factor Bax in 14 sporadic vestibular schwannomas , and related the findings to the MIB 1 labeling index as a marker for cell proliferation . ^^^ Bcl 2 expression was found in the cytoplasm of 9 tumors ( 64 % ) , and Bax was found in 10 out of 14 schwannomas ( 71 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The downregulated bcl 2 expression ( all P < 0 . 05 in 2 and 4 wk ) and the upregulated bax expression ( P < 0 . 01 in 2 wk ; P < 0 . 05 in 4 wk ) were detected by immunohistochemistry , in comparison with control groups . ^^^ Gene bcl 2 and bax protein expression were consistent with the suppression of intimal proliferation and the enlargement of luminal areas in corresponding sections . ^^^ CONCLUSION : As ( 2 ) O ( 3 ) induces apoptosis of VSMCs and inhibits experimental restenosis effectively after artery injury , via downregulation of bcl 2 and upregulation of bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Before IL 2 culture , TIL showed undetectable or very low levels of T cell receptor ( TCR ) epsilon chain , p 56 ( lck ) , Fas ligand ( FasL ) and Bax expression , while Bcl 2 values were elevated . ^^^ Cancer cells were characterised by low or absent Fas and Bcl 2 and high Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although concentrations of Bcl 2 , Bax , FLICE inhibitory protein ( FLIP ) , and Fas ligand were unaffected by MG 132 or actinomycin D , both treatments led to a significant increase of p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the cold storage , there was no significant change in the Bcl 2 to Bax protein ratio , mitochondrial location of cytochrome C or caspse 3 activity . ^^^ However , during rewarming , the Bcl 2 to Bax ratio increased , cytochrome C was translocated to cytosol , and caspase 3 was activated : events and timing were consistent with the occurrence of apoptosis during rewarming . ^^^ Addition of antioxidants ( deferoxamine or 2 methyaminochroman ) to the storage solution suppressed mitochondrial pore opening and swelling , Bcl 2 to Bax ratio increase , cytochrome C translocation , caspase 3 activation as well as rewarming induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aortic arch of each animal was sectioned and stained with antibodies against SMC , endothelial cells , macrophages and oxidised LDLs or for proteins involved in apoptotic pathways such as Fas , Bax , Bcl 2 , and caspase 3 . ^^^ Fas , Bax and caspase 3 signals were mainly located in the endothelium and SMC proximal to the lumen , whereas Bcl 2 colocalized with macrophages and SMC deeper in the lesions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was quantified by DEVDase ( caspase 3 like ) activity assay and immunoblot analysis of apoptosis regulatory proteins ( Bax and Bcl 2 ) . ^^^ As a mechanism of cell deletion consistent with apoptosis induction , ventricles showed increased caspase 3 activation ( > 4 . 5 fold ) as well as Bax to Bcl 2 protein ratio ( > 3 . 2 fold ) within 2 weeks of valsartan or enalapril treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 family proteins ( Bcl 2 , Bax and Bcl 10 ( L ) ) was analyzed by flow cytometry showing high expression of the three proteins which was not significantly modified after treatment with either PSC 833 or CsA on the sensitive as well as on the resistant cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting demonstrated that , although expression of Bax and Bcl 2 were not changed by heat shock , p 53 expression was elevated in LK 2 , but not LU65A cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of 17 AAG induced apoptosis : role of Bcl 2 , Bcl XL , and Bax downstream of 17 AAG mediated down regulation of Akt , Raf 1 , and Src kinases . 17 allylamino demethoxy geldanamycin ( 17 AAG ) inhibits the chaperone function of heat shock protein 90 ( Hsp 90 ) and promotes the proteasomal degradation of its misfolded client proteins . ^^^ Treatment with 17 AAG triggered the B cell lymphoma 2 ( Bcl 2 ) associated 10 protein ( Bax ) conformational change associated with apoptosis , while Bax deficient cells were resistant to 17 AAG induced apoptosis . ^^^ In addition , in HL 60 / Bcl 2 and HL 60 / Bcl xL cells , which ectopically express Bcl 2 and Bcl xL respectively , 17 AAG induced Bax conformational change , cytosolic accumulation of cyt c and Smac / DIABLO , and apoptosis were markedly inhibited . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transient SMC apoptosis occurred in the aortic media with a peak around day 5 of treatment , with increases in the Bax to Bcl 2 protein ratio ( > 3 fold ) , in active caspase 3 ( 5 . 6 fold ) , in TUNEL positive nuclei ( 19 fold ) , preceding by 24 hours the peak activation of capase 9 ( 3 . 8 fold ) , and significant reductions in SMC number ( 46 % ) and aortic cross sectional area ( 8 . 5 % ) at 5 . 5 days . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of Bid induced apoptosis by Bcl 2 . tBid insertion , Bax translocation , and Bax / Bak oligomerization suppressed . ^^^ Bcl 2 also prevented Bid induced Bax translocation from cytosol to the membrane bound organellar fraction . ^^^ Finally , Bcl 2 diminished Bid induced oligomerization of Bax and Bak within the membrane bound organellar fraction , shown by cross linking experiments . ^^^ Critical steps blocked by Bcl 2 included tBid insertion , Bax translocation , and Bax / Bak oligomerization in the mitochondrial membranes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The inner ears were examined with TUNEL ( in situ terminal dUTP nick end labeling ) and immunocytochemistry of the apoptosis regulatory proteins Bcl 2 and Bax . ^^^ Moreover , no cells staining positive for Bcl 2 and Bax are found in any structures of the inner ears . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was also evident that the expression of Bax was increased , whereas the level of Bcl 2 was decreased , in glioma cells treated with the supernatant from the activated macrophages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High toxic concentration of DA ( 500 microM ) , R APO ( 50 microM ) , melatonin ( 50 microM ) , and EGCG ( 50 microM ) exhibited a similar profile of proapoptotic gene expression , increasing the level of bax , caspase 6 , fas ligand , and the cell cycle inhibitor gadd 45 genes , while decreasing antiapoptotic bcl 2 and bcl xL . ^^^ Melatonin displayed an extremely low index of mortality , which may be partially explained by the observation that a high concentration did not significantly affect the expression of mitochondrial Bcl 2 family members , bcl 2 and bax . ^^^ Protein analysis of Bcl 2 , Bax , and activated caspase 3 correlated with the gene expression pattern . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
KR 31372 and diazoxide enhanced DNA fragmentation associated with increase in phosphatase and tensin homolog deleted from chromosome 10 ( PTEN ) and decrease in serine / threonine kinase phosphorylation , which were accompanied by augmented Bax and cytochrome c release , and suppressed Bcl 2 in HUVECs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a potent pro apoptotic member of the Bcl 2 protein family that localizes to the mitochondrial membrane during apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , F 11782 induced apoptosis in P 388 cells was associated with an increased expression of the pro apototic Bax protein , without significant changes in the level of the anti apoptotic Bcl 2 protein , and with modification at the mitochondrial membrane function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that p 53 and Bax expression were dramatically increased at the same time points , whereas Bcl 2 expression was significantly reduced at all time points after deltamethrin treatment . ^^^ Further , we found that nitric oxide synthase inhibitor N ( G ) nitro L arginine prevented deltamethrin induced neuronal apoptosis and altered expression of p 53 , Bax , and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , to investigate whether Bcl 2 and / or Bax is involved in the mechanism of leptin induced adipose tissue apoptosis . ^^^ MEASUREMENTS : DNA fragmentation and Bcl 2 and Bax protein levels were determined in inguinal ( ING ) , epididymal ( EPI ) and retroperitoneal ( RP ) white adipose tissues and the interscapular brown adipose tissue ( BAT ) . ^^^ The other fat pads exhibited a general trend to increase the Bcl 2 / Bax ratio . ^^^ After recovery , apoptosis was not detected , Bcl 2 protein had increased in ING , and the Bcl 2 / Bax ratio had risen overall . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : In a synergistic fashion , Gem 231 and HCPT induced growth arrest , apoptosis , and changes in cell morphology ; down regulated RIalpha expression ; down regulated Bcl 2 and promoted its hyperphosphorylation ; up regulated the proapoptotic proteins Bax and Bad ; and promoted hypophosphorylation of Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cell lysates were analyzed by immunoblotting techniques for the expression of key genes targeted by ciprofloxacin ( p21WAF1 , Bax and Bcl 2 ) . ^^^ There was a significant increase in the Bax / Bcl 2 ratio with translocation of Bax , a pro apoptotic protein , to mitochondria with concomitant activation of caspase 3 . ^^^ These effects of ciprofloxacin were mediated by cell cycle arrest at S G2 / M phase of the cell cycle , Bax translocation to mitochondrial membrane and by increasing the Bax / Bcl 2 ratio in PC 3 prostate cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of Bcl 2 and Bax may enhance neuroblastoma survival . ^^^ It was hypothesized that the expression of proapoptotic ( Bax ) and prosurvival ( Bcl 2 and Mcl 1 ) proteins would be altered in neuroblastoma cells grown in a cell culture model of metastatic neuroblastoma . ^^^ Bcl 2 , Mcl 1 , and Bax mRNA were measured with reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ CONCLUSIONS : When neuroblastoma cells placed in coculture , the prosurvival protein , Bcl 2 , is upregulated whereas the proapoptotic protein , Bax , is downregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To investigate the apoptosis in esophageal cancer cells induced by resveratrol , and the relation between this apoptosis and expression of Bcl 2 and Bax . ^^^ Immunohistochemical staining was used to detect the expression of apoptosis regulated gene Bcl 2 and Bax . ^^^ Immunohistochemical staining showed that after the treatment of EC 9706 cells with resveratrol ( 10 mmol / L ) for 24 to 96 hours , the PRs of Bcl 2 proteins were apparently reduced with treated time ( P < 0 . 05 ) and the PRs of Bax proteins were apparently increased with treated time ( P < 0 . 05 ) . ^^^ This apoptosis may be mediated by down regulating the apoptosis regulated gene Bcl 2 and up regulating the expression of apoptosis regulated gene bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To investigate the apoptosis in gastric cancer cells induced by paclitaxel , and the relation between this apoptosis and expression of Bcl 2 and Bax . ^^^ Immunohistochemical staining was used to detect the expression of apoptosis regulated gene Bcl 2 and Bax . ^^^ Paclitaxel could reduce the expression of apoptosis regulated gene Bcl 2 , and improve the expression of apoptosis regulated gene Bax . ^^^ This apoptosis may be mediated by down expression of apoptosis regulated gene Bcl 2 and up expression of apoptosis regulated gene Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Precursors from aged animals were deficient in ability to modulate expression of apoptosis regulatory genes Bax , Bcl 2 , and Bcl 10 in comparison to B cell precursors from young animals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An immunohistochemical study of proteins linked with cell death and cell survival demonstrated reduced expression of Fas , Fas L , Bcl 2 and Bax in swollen motor neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical analysis of Bax , Bcl 2 , and Bcl 10 ( L ) was performed in tissue specimens of patients with RA and compared to normal synovial tissue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TGF beta also increased the intracellular level of peroxides measured with carboxydichlorodihydrofluorescein , but it did not affect the protein levels of Bcl 2 , Bax , Bad , or p 53 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
They interact with the anti apoptotic Bcl 2 family members and induce apoptosis by a mechanism that requires the presence of at least one of the multidomain pro apoptotic proteins Bax or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Investigation of the mitochondrial apoptosis pathway showed a consistent pro apoptosis profile , namely , upregulation of Bax , Bax alpha , cytochrome c , and Apaf 1 , and low bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A similar result was obtained upon the substitution of glycine 145 with alanine in the BH 1 domain ( Bcl 2 / G145A ) , which failed to interact with either Bax or Bak . ^^^ Pro apoptotic Bax and Bak have been known to be activated in response to antitumor drugs , and Bcl 2 / G145A as well as Bcl 2 / DeltaBH1 also accelerated Bax or Bak induced apoptosis in HEK293T cells . ^^^ These two mutants still retained the ability to interact with wild type Bcl 2 and Bcl xL , and abrogated the inhibitory effect of wild type Bcl 2 or Bcl xL on Bax or Bak induced apoptosis . ^^^ In addition , immunoprecipitation studies revealed that Bcl 2 / DeltaBH1 and Bcl 2 / G145A interrupted the association between wild type Bcl 2 and Bax / Bak . ^^^ Taken together , our results demonstrate that Bcl 2 / DeltaBH1 or Bcl 2 / G145A acts as a dominant negative of endogenous anti apoptotic proteins such as Bcl 2 and Bcl xL , thereby enhancing antitumor drug induced apoptosis , and that this dominant negative activity requires both a failure of interaction with Bax and Bak through the BH 1 domain of Bcl 2 and retention of the ability to interact with Bcl 2 and Bcl xL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Evaluation of clinical significance of TP 53 , BCL 2 , BAX and MEK 1 expression in 229 ovarian carcinomas treated with platinum based regimen . ^^^ In cell line studies , BCL 2 , BAX , as well as novel MEK 1 protein levels have strong influence on ovarian cancer response to cisplatin based chemotherapy . ^^^ Another novel finding is that CR was negatively influenced by high BAX expression in all patients group ( P=0 . 047 ) and by BCL 2 expression in the TP 53 ( ) group ( P=0 . 05 ) . ^^^ BCL 2 and BAX , but not MEK 1 expressions have predictive value in ovarian cancer patients treated with platinum based chemotherapy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MPP ( + ) increased levels of Bax , Bcl 2 and Bcl 10 ( L ) proteins approximately 2 fold over 24 hr , with Bax increases occurring first ; Bax did not increase in rho ( 0 ) cells . ^^^ The Bax increase , but not that of Bcl 2 or Bcl 10 ( L ) , was dependent on nitric oxide ( NO ) and seemed post transcriptional . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The long term NO generator diethylenetriamine NO ( DETA NO ) reproduced the post transcriptional Bax protein increase , but did not increase Bcl 2 or Bcl 10 ( L ) proteins . ^^^ Like MPP ( + ) , DETA NO caused an early decrease in Bcl 2 mRNA , did not increase Bax protein in rho ( 0 ) cells and caused caspase and cycloheximide dependent apoptosis and caspase independent cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in other apoptotic proteins , including Bcl 2 , Bax , Mcl 1 , and 10 linked inhibitor of apoptosis ( XIAP ) , were not observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Murine endothelial cells and human T lymphocytes lacking PECAM 1 were found to be far more sensitive than their PECAM 1 expressing counterparts to multiple death signals that stimulate Bax , a multidomain , proapoptotic member of the Bcl 2 family that plays a central role in mitochondrial dysfunction dependent apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We performed double stainings of BAFF together with one of the markers , CD 21 , CD 68 , CD 40 , Fas , Bcl 2 or Bax , and monitored apoptosis among BAFF expressing cells by using the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate digoxigenin nick end labeling method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Urinary outflow obstruction increases apoptosis and deregulates Bcl 2 and Bax expression in the fetal ovine bladder . ^^^ While 30 days of obstruction in utero between 75 and 105 days gestation resulted in overall growth of the fetal bladder as assessed by weight , protein , and DNA measurements , we found that apoptosis , as assessed by in situ end labeling , was up regulated in fetal bladder detrusor muscle and lamina propria cells and that this was accompanied by a down regulation of the anti death protein Bcl 2 and an up regulation of the pro death protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cytosolic domain of BCL w consists of 8 alpha helices , which adopt a fold similar to that of BCL xL , BCL 2 , and BAX proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Modulation of pro apoptotic ( Bax ) and anti apoptotic ( Bcl 2 ) gene expression in isolated porcine hepatocytes perfused within a radial flow bioreactor after low temperature storing . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) was used in order to amplify specific mRNAs for Bcl 2 and Bax genes , by using appropriate primers ; beta actin primers were used as control . ^^^ Total RNA was extracted by northern blotting analysis and for Bcl 2 , Bax and beta actin RNA messenger detection , RT PCR amplification was used . ^^^ Freshly isolated hepatocytes perfused into the RFB showed a progressive increase of MEGX while a loss in Bax expression was paralleled by an increase in Bcl 2 expression , in comparison to starting hepatocytes . ^^^ After 4 degrees C storage and warm reperfusion , MEGX production was preserved in 24 h and 48 h stored bioreactors as well as a sharp increase of Bcl 2 and a decrease of Bax mRNAs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This review focuses on novel strategies to induce apoptosis in glioma cells by transduction with adenoviral vectors carrying a variety of apoptosis related genes , including Fas ligand , Fas , FADD , caspase 8 , p 53 , p33ING1 , p73alpha , Bax , Apaf 1 , caspase 9 , IkappaBdN , caspase 3 , Bcl 2 , and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that the expression of apoptosis related proteins p 53 , C myc , and Bax were up regulated whereas the expression of Bcl 2 was down regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The paraquat mediated gene or protein expression of proapoptotic Bax , Bcl w , and Bcl 10 ( S ) , cell survival / death factors GADD 45 , MDM 2 , c Myc , and caspase 3 was upregulated , but that of antiapoptotic Bcl 2 was downregulated in the GPX 1 / mice vs . the WT mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After 24 h occlusion , the hearts of the surviving rats ( group F : n=13 , group C : n=10 ) were excised to assess minimum infarct wall thickness and infarct size , determine the number of TUNEL positive cardiomyocytes and to analyze Bcl 2 and Bax expression by immunohistochemical staining and Western blotting . ^^^ Western blot analysis showed greater expression of Bcl 2 in group F than in group C ( p < 0 . 05 ) , but similar expression of Bax in the 2 groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Molecular markers of outcome after radiotherapy in patients with prostate carcinoma : Ki 67 , bcl 2 , bax , and bcl 10 . ^^^ The current study examined the association between expression levels of Ki 67 , bcl 2 , bax , and bcl 10 in pretreatment biopsy specimens and patient outcome after definitive radiotherapy alone . ^^^ Expression levels of Ki 67 ( MIB 1 staining ; n = 106 patients ) , bcl 2 ( n = 77 patients ) , bax ( n = 70 patients ) , and bcl 10 ( both long and short splice variants ; n = 72 patients ) were determined by immunohistochemical staining . ^^^ RESULTS : High Ki 67 LI ( > 3 . 5 % ) expression was observed in 33 % of patients , overexpression of bcl 2 was observed in 16 % of patients , altered bax expression was observed in 23 % of patients , and altered bcl 10 expression was observed in 53 % of patients . ^^^ Kaplan Meier survival estimates of freedom from biochemical failure ( bNED ) and the log rank test revealed significantly lower rates in association with high Ki 67 LI , positive bcl 2 , and altered bax staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objective of this study was to access the prognostic significance of the expression of proliferating cell nuclear antigen ( PCNA ) and the apoptosis related genes ( bax , bcl 2 , and p 53 ) in laryngeal carcinoma patients . ^^^ We immunohistochemically studied the expression of PCNA and bax , bcl 2 , and p 53 genes in their tumor specimens . ^^^ Similarly , the presence of PCNA led to an unfavorable clinical outcome in those patients who were bax negative , bcl 2 negative , and p 53 negative ( 0 % versus 50 % , p = . 0278 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The activation of caspases after brain ischaemia is largely consequent on the translocation of Bax , Bak , and other BH 3 only members of the Bcl 2 family to the mitochondrial outer membrane and the release of cytochrome c , procaspase 9 , and apoptosis activating factor 1 ( Apaf 1 ) from the mitochondrial intermembrane space . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have screened oocytes and embryos for a panel of genes including beta actin ( expressed in 24 / 28 oocytes , 6 / 6 pronuclear embryos ) , the integrins beta 1 ( 17 / 28 oocytes , 6 / 6 pronuclear embryos ) and beta 5 ( 8 / 28 oocytes , 5 / 6 pronuclear embryos ) , and the apoptotic regulators BCL 2 ( 20 / 28 oocytes , 2 / 6 pronuclear embryos ) and BAX ( 21 / 28 oocytes , 5 / 6 pronuclear embryos ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whether conventional hypothermic CPB induces myocyte apoptosis in dog hearts and modulation of bcl 2 , bcl xl , bax , bad , and caspase 3 pathways in this setting was investigated . ^^^ Immunohistochemistry and flow cytometry were employed for detection of expressions of bcl 2 , bcl xl , bax and bad proteins . ^^^ The results of immunohistochemistry demonstrated that bcl 2 , bcl xl , bax and bad proteins were constitutionally present on the sarcolemma of the LV myocytes . ^^^ FACS results showed that , after CPB , expressions of bax and bad in CPB group were significantly upregulated , while the expressions of bcl 2 and bcl xl were not significantly changed in both groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NO 2 in supernate , the proliferation and apoptosis of TM cells and mRNA expression of bcl 2 and bax were measured by Griess reagent , terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) , MTT assay and in situ hybridization , respectively . ^^^ The results showed that L arginine with concentration > or = 1 10 10 ( 4 ) mol / L could induce apoptosis of the TM cells and inhibit the proliferation of TM cells through increasing the NO levels , down regulating bcl 2 mRNA expression and up regulating bax mRNA expression ; L NAME with concentration > or = 1 10 10 ( 5 ) mol / L could induce the proliferation of the TM cells through suppressing the production of NO . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Excessive apoptosis is an undisputed mechanism in these processes , mediated by decreased expression of apoptosis inhibiting genes ( Bcl 2 , HGF , IGF , BMP 7 ) , and overexpression of pro apoptotic genes like Bax and TGF beta . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To clarify the basis for cell killing during prostate cancer radiotherapy , we determined the IR induced expression of several apoptotic ( bax , bcl 2 , survivin and PARP ) and G 1 cell cycle checkpoint ( p 53 and p 21 ( WAF1 / Cip1 ) ) related proteins , in both normal ( PrEC epithelial and PrSC stromal ) and malignant ( LNCaP , DU 145 and PC 3 ; all epithelial ) prostate cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This dose of HupA also reduced the upregulation of the pro apoptotic gene , bax , downregulation of the anti apoptotic gene , bcl 2 , and antagonized the decrease in immunoreactive caspase 3 proenzyme . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To dissect apoptotic genes governing the survival of colorectal carcinoma cells , we employed RNAi to silence Bcl 2 and Bcl 10 ( L ) in isogenic clones of p53+ / + and p 53 / cells , and of Bax+ / and Bax / cells . ^^^ The `` Bcl 2 / p53 axis ' ' requires Bax and caspase 2 as essential apoptotic mediators . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a crucial mediator of the mitochondrial pathway for apoptosis , and loss of this proapoptotic Bcl 2 family protein contributes to drug resistance in human cancers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of BID , a `` BH 3 domain only ' ' Bcl 2 family member , triggers the oligomerization of proapoptotic family members Bak or Bax , resulting in the release of mitochondrial proteins to cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine p 75 ( NTR ) dependent apoptosis in tumor cells , we demonstrated that a dose dependent increase in p 75 ( NTR ) expression was associated with a concomitant increase in the mitochondrial proapoptotic effector proteins Bad , Bax and Bik and a decrease in the mitochondrial prosurvival effector proteins phospho Bad , Bcl 2 and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antioxidants , ( ) epigallocatechin gallate and quercetin , inhibited endothelial apoptosis , enhanced the expression of bcl 2 protein and inhibited the expression of bax protein and the cleavage and activation of caspase 3 . ^^^ The antiapoptotic activity of flavonoids appears to be mediated at the mitochondrial bcl 2 and bax gene level . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate the interaction between gastrin , cyclooxygenase 2 ( COX 2 ) , hepatocyte growth factor ( HGF ) and apoptosis related proteins ( Bax , Bcl 2 , caspase 3 , survivin ) in cultured gastric epithelial cancer cells . ^^^ Using RT PCR and Western blot , we examined the mRNA and protein expression for COX 2 , HGF and apoptosis related proteins ( Bax , Bcl 2 , caspase 3 and survivin ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate whether the Bcl 2 gene family is involved in modulating mechanism of apoptosis and change of cell cycle protein induced by curcumin in acute myeloid leukemia HL 60 cell line and primary acute myelogenous leukemic cells , the Bcl 2 family member Mcl 1 , Bax and Bak and cell cycle proteins including P27kipl , P21wafl , cyclin D 3 and pRbp were selected and their expression detected by SABC immuno histochemical stain method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of ginsenoside Re on cardiomyocyte apoptosis and expression of Bcl 2 / Bax gene after ischemia and reperfusion in rats . ^^^ To observe the effect of ginsenoside Re on cardiomyocyte apoptosis and Bcl 2 / Bax gene expression after ischemia ( 30 min ) and reperfusion ( 6 h ) in rats and to elucidate the possible mechanisms of ginsenoside Re on inhibition of cardiomyocyte apoptosis , the ischemia / reperfusion heart model was established by ligating the left anterior descending branch of coronary artery in Wistar rats . ^^^ The mRNA and protein expression of Bcl 2 and Bax genes were studied by in situ hybridization and immunohistochemical staining . ^^^ The differences was significant between two groups ( P < 0 . 01 ) ; ( 3 ) Gene expression of Bcl 2 and Bax were increased significantly in the ischemia / reperfusion group and ginsenoside Re treated group when compared with the sham operation group . ^^^ There was no significant difference in the gene expression of Bcl 2 between the ginsenoside Re treated group and ischemia / reperfusion group ( P > 0 . 05 ) , but gene expression of Bax was decreased significantly in the ginsenoside Re treated group as compared with the ischemia / reperfusion group ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transcription factor GATA 6 , cell proliferation , apoptosis , and apoptosis related proteins Bcl 2 and Bax in human fetal testis . ^^^ We have studied GATA 6 expression in human fetal testis using in situ hybridization and immunohistochemistry and compared these results with the expression of the apoptosis related proteins Bcl 2 and Bax . ^^^ Bax was present throughout the fetal period , whereas Bcl 2 expression decreased toward term . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of PARP , Bcl 2 and Bax were examined by Western blot assay . ^^^ After 48 hours incubation with tributyrin at 2 mmol / L ( 1 ) , the level of Bcl 2 protein was lowered , and the level of Bax protein was increased in SGC 7901 , accompanied by PARP cleavage . ^^^ CONCLUSION : Tributyrin could inhibit the growth of gastric cancer cells effectively in vitro by inhibiting DNA synthesis and inducing apoptosis , which was associated with the down regulated Bcl 2 expression and the up regulated Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunofluorometric study of Bcl 2 and Bax expression in clinical fresh tumor samples from breast cancer patients . ^^^ BACKGROUND : The balance between the expression of the antiapoptotic gene Bcl 2 and the proapoptotic gene Bax is considered a good indicator of the apoptotic activity of tumor cells . ^^^ Bcl 2 and Bax expression seem also to individually play a prognostic role in breast cancer . ^^^ MATERIALS AND METHODS : Fresh tumor specimens from 86 breast cancer patients were studied by means of immunofluorocytometry for the expression of the apoptosis associated Bcl 2 and Bax genes . ^^^ Bax expression did not correlate with any other clinical or biological parameters of the tumors , including Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Aberrant bcl 2 and bax protein expression related to chemotherapy response in neuroblastoma . ^^^ In this study we investigated the immunocytochemical expression , before and after a trial of chemotherapy of the bcl 2 and bax proteins in 15 cases of NB , including 8 stroma poor and 7 stroma rich NBs . ^^^ We conclude that bcl 2 and bax expression , before the treatment , could be of prognostic value in neuroblastomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Germ cell apoptosis and expression of Bcl 2 and Bax following testicular torsion / detorsion in rats ] . ^^^ OBJECTIVES : To investigate the relationship between germ cell apoptosis and expression of Bcl 2 and Bax in experimental torsed / detorsed testes of the adult male rats . ^^^ The expression of Bcl 2 and Bax were detected by immunohistochemical method . ^^^ RESULTS : In the torsed testes , the apoptosis index of germ cell and Bax expression significantly increased compared with that in the control group ( P < 0 . 01 ) while Bcl 2 expression obviously decreased ( P < 0 . 01 ) . ^^^ CONCLUSIONS : The germ cell apoptosis is highly associated with expression of Bcl 2 and Bax in experimental testicular torsion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We therefore developed a four color flow cytometry method that enables establishment of apoptosis related protein expression such as Bcl 2 , Bcl 10 ( L ) , Mcl 1 and Bax at diagnosis and in MRD . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
LPA prevented translocation of Bax from cytosol to mitochondria and increased the expression of the antiapoptotic Bcl 2 mRNA and protein . ^^^ These data indicate that LPA protects IEC 6 cells from camptothecin induced apoptosis through G ( 1 ) coupled inhibition of caspase 3 activation mediated by the attenuation of caspase 9 activation due to diminished cytochrome c release , involving upregulation of Bcl 2 protein expression and prevention of Bax translocation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , LDFRT treatment in both cell lines with or without Paclitaxel down regulated nuclear factor kappa B activity and BCL 2 protein expression and simultaneously up regulated BAX protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BH 3 ( Bcl 2 homology 3 ) only proteins of the Bcl 2 family activate Bax or Bak during apoptosis to promote the release of pro death factors sequestered in the mitochondrial intermembrane space . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To address this issue , we have assessed the effects of the anticancer agent doxorubicin ( DX ) on a panel of human tumor cell lines originally derived from untreated patients and tried to establish a correlation between cell response and a number of parameters , including drug accumulation and / or drug efflux ; differences in expression and / or subcellular distribution of proteins involved in the apoptotic process ( e . g . , p 53 , Bcl 2 , Bax ) and intracellular signal transducers ( PKCalpha ) ; changes in key detoxification processes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hearts were investigated using stereological methods , electron microscopy , immunohistology for the proliferation marker proliferating cell nuclear antigen , the pro and anti apoptotic proteins Bax and Bcl 2 as well as the TUNEL technique . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These changes are suppressed by Bcl 2 / Bcl XL facilitated by Bax , and mediated at least by so called permeability transition pore complex which is one of possible mechanisms involved in mitochondrial membrane permeabilization ( MMP ) . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was used on paraffin sections to detect LMP 1 / EBV , CD 15 and the apoptosis related proteins ( bcl 2 , bax , bcl 10 , mcl 1 and CD 95 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , examination of the levels of apoptosis regulatory proteins showed that , while levels of p 53 , Bax and p 21 are higher , that of anti apoptotic Bcl 2 is undetectable in cells treated with 2 ME compared with untreated controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of HSP 72 , B cell lymphoma gene product 2 ( Bcl 2 ) and Bax were evaluated by Western blot analysis . ^^^ The protective effects of the induction of HSP 72 were associated with an increased renal Bcl 2 / Bax ratio and the reduction of TUNEL positive cells in the outer stripe of outer medulla . ^^^ The protective effects of HSP 72 are associated with an increased Bcl 2 / Bax ratio and less apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Samples of normal sigmoid colon mucosa , obtained by colonoscopic check once a year during the intervention period , were used to investigate COX 2 , cell proliferation ( Ki 67 expression ) , p 53 , Bcl 2 and Bax by immunostaining and determine the apoptosis index ( AI ) by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling ( TUNEL ) in 21 and 20 patients in experimental and comparison groups , respectively , who completed the 2 years of the intervention . ^^^ After 24 months , the AI and positive cells of Bax and the ratio of Bax / Bcl 2 in normal sigmoid colon mucosa for the experimental group was significantly increased , whereas this change was not found in comparison group . ^^^ These observations demonstrated for the first time that increased intake of n 3 PUFAs promotes apoptosis of normal colon mucosa in human which is related to effect on Bax or the balance of Bax and Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , altered basal expression patterns of Bcl 2 and Bax , two key regulators of apoptosis , were found in the LFS lymphocytes when compared with controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Calcitriol induced cell death was regulated by members of the Bcl 2 family of apoptosis regulatory proteins , as shown by calcitriol induced up regulation of proapoptotic Bax and Bak and the lack of calcitriol induced cytotoxicity in Bcl 2 overexpressing insulinoma cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No significant difference was observed in the expression of Bcl 2 family members ( Bcl 2 , Bax ) between Turner ' s syndrome cord blood and normal cord blood lymphocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic members , like Bax , contain three of the four Bcl 2 homology regions ( BH 1 3 ) , while BH 3 only proteins , like Bim , possess only the short BH 3 motif . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we show that Cidofovir induces a downregulation of the EBV oncoprotein LMP 1 associated with a decrease of the antiapoptotic Bcl 2 and an increase of the proapoptotic Bax protein in Raji ( BL ) and C 15 ( NPC ) cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This lithium protection was correlated with up regulation of cytoprotective Bcl 2 and down regulation of apoptotic proteins p 53 and Bax , and neurons showing DNA damage and caspase 3 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The issue of whether the Fas and Fas ligand ( FasL ) system and / or the Bax and Bcl 2 system is involved in the induction of germ cell apoptosis in normal and damaged testes will then be addressed , including a special consideration of the ischemia reperfusion model , the endocrine disruptor treated model , and others . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results revealed that the expression of bcl 2 and bcl 10 ( L ) decreased after 5 and 3 h p . i . , respectively ; while bax and procaspase 3 expression increased with respect to control as a function of p . i . times and at 7 h p . i . they were not observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We observed downregulation of the expression of inhibitors of apoptosis proteins ( IAPs ) Bcl 2 , Bcl 10 ( L ) , and survivin and upregulation of pro apoptotic p 53 and Bax as assessed by Western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study sought to describe the expression of survivin in a cohort of 90 benign meningiomas , together with the pattern of expression of other genes involved in the apoptotic process , namely bax and bcl 2 . ^^^ Survivin expression was noted in 94 % ( 85 / 90 ) of samples and was not correlated with the expression of either bax or bcl 2 or with clinicopathological factors . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , evodiamine increased the expression of the apoptosis inducer Bax , but decreased the expression of the apoptosis suppressor Bcl 2 in mitochondria . ^^^ Taken together , our data indicated that evodiamine alters the balance of Bcl 2 and Bax gene expression and induces apoptosis through the caspase pathway in HeLa cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Assessments were made of neurotrophic factors nerve growth factor , brain derived neurotrophic factor , neurotrophin 3 , and neurotrophin 4 ; apoptosis related proteins Bcl 2 , Bcl xl , Bax , Bcl xs , Bad , phosphorylated Bad , phosphorylated Akt , and phosphorylated c Jun N terminal kinase ; and the antioxidants superoxide dismutase , glutathione reductase , and catalase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) assay was used to determine apoptosis as the mechanism of cytotoxicity ; alterations in bcl 2 and bax expression were determined using Western blot analysis . ^^^ The combination of a PKC inhibitor with CPT 11 or SN 38 led to decreased expression of the antiapoptotic protein bcl 2 , and increased expression of the proapoptotic protein bax . ^^^ The induction of apoptosis was secondary to a decrease in bcl 2 and an increase in bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We followed body weight gain with growth , measured cardiac hypertrophy after a 2 wk swim exercise program , markers of apoptosis ( Bcl 2 , BAX , BNIP 1 , caspase 3 activation ) , oxidative stress ( heme oxygenase 1 , protein carbonyl levels ) , the chaperone protein clusterin , and the transcriptional activator early growth response gene 1 ( Egr 1 ) in hearts of nonexercised and exercised rats on neonate day 38 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using RNase protection assays , we showed that IL 6 enhanced Fas induced apoptosis and expression of Bax in normal Fb , but inhibited apoptosis and induced expression of Bcl 2 in IPF Fb . ^^^ Densitometry of Western blots revealed a Bcl 2 / Bax ratio 0 . 15 + / 0 . 01 in normal Fb compared with 12 . 05 + / 1 . 0 in IPF Fb . ^^^ Upregulation of Bcl 2 in normal Fb and Bax in IPF Fb were both STAT 3 dependent . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After 24 hours , apoptosis , mitochondrial membrane integrity , and Bcl 2 and Bax expression were immunocytochemically determined . ^^^ Neuronal apoptosis was accompanied by mitochondrial membrane depolarization , an increase in the amount of photoreceptors expressing Bax , and a decrease in those expressing Bcl 2 . ^^^ DHA blocked the paraquat induced increase in Bax expression and remarkably upregulated Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the apoptosis related proteins p 53 , p 21 , Rb , bcl 2 , bax , c myc , Fas and Fas L was assessed immunohistochemically . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 overexpression did not alter overall Bax expression , but inhibited cytosolic accumulation of cytochrome c and caspase 3 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the 7th , 14th and 28th day after the treatment , the hepatocellular carcinoma nodules were obtained and the morphologic changes of hepatocellular carcinoma cells were observed under light and electron microscopes ; Immunohistochemistry ( S P methods ) was employed to detect the expression of bcl 2 , bax and PCNA in hepatocellular carcinoma tissues ; flow cytometry ( TUNEL assay ) was used to detect the apoptosis of liver cancer cells and the change of cytokinetics . ^^^ The expression of bcl 2 and bax was related to the dose of As ( 2 ) O ( 3 ) . ^^^ But the incidence of apoptosis was not the highest status and the ratio of bcl 2 / bax was at the lowest when the highest dose of As ( 2 ) O ( 3 ) was used . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Three days to 12 weeks after MPTP treatment , a detectable reduction of tyrosine hydroxylase immunoreactivity in the amacrine cells was observed , with an increase of Bcl 2 expression in the Mller glial cells , and a de novo expression of Bad and Bax in the retinal ganglion cells , optic nerve fibers and plexiform layers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
They communicate this information to the pro apoptotic ' multidomain ' members Bax or Bak a process that is antagonized by anti apoptotic members of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since Bcl 2 family proteins are key regulators of apoptosis , we examined the effects of H2O2 on the expression of principal Bcl 2 family proteins ( Bcl 2 , Bcl xL , Bax , Bad ) in neonatal rat cardiac myocytes . ^^^ Bcl 2 , Bax , and Bad were all down regulated in myocytes exposed to 0 . 2 mm H2O2 , a concentration that induces apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic index , the rate of proliferation , and the expression of the anti apoptotic Bcl 2 protein and pro apoptotic Bax protein were assessed in surgically excised tumours . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The GAPDH binding is associated with decreased synthesis of pro apoptotic proteins like BAX , c JUN and GAPDH but increased synthesis of anti apoptotic proteins like BCL 2 , Cu Zn superoxide dismutase and heat shock protein 70 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of the intrinsic ( mitochondrial ) pathway following focal ischemia is triggered by Bax translocation to and competition with Bcl 2 and other members of the Bcl 2 family in the mitochondria membrane that is followed by cytochrome c release to the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , tea and tea pigments inhibited the expression of Bcl 2 protein and induced the expression of Bax protein ( P < 0 . 05 or P < 0 . 01 ) . ^^^ The study provided evidence supporting that PCNA LI , AgNORs , Bcl 2 , Bax and ras p 21 protein could be used as effective biomarkers for colorectal carcinogenesis in human chemopreventive trials . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 , bax , and proliferation cell nuclear antigen ( PCNA ) of rat liver cancer cells on the 7th day after drug administration was determined by using immunohistochemical technique . ^^^ The expression intensity of bcl 2 or bax varied depending on the dose administrated . ^^^ Downregulation of bcl 2 / bax was observed , accompanied with upregulation of apoptosis . ^^^ However , the ratio of bcl 2 / bax and the percentage of apoptosis were not the utmost when the dose administered was the highest . ^^^ Apoptosis of tumor cells is closely associated with down regulation of the ratio of bcl 2 / bax , but that may not be the only dominant factor . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exceptionally , the one female injected with the IL R peptides showed a low level of the liver TGF alpha cDNA together with the pancreatic expressions of Bax ( 140 bp ) , bcl 2 and like interleukin converting enzyme ( LICE ) ( 318 bp ) cDNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax and p 53 proteins in pituitary adenomas : an immunohistochemical study . ^^^ The purpose of this study was to investigate the expression of apoptosis related proteins including Bcl 2 , bax and p 53 in pituitary adenomas and its correlation with hormone function , tumor size , local control , and proliferative activity . ^^^ STUDY DESIGN : The expression of Bcl 2 , Bax and p 53 proteins and hormonal function were determined in formalin fixed , paraffin embedded tissue from 41 untreated pituitary adenomas using immunohistochemistry . ^^^ Immunohistochemically , 31 adenomas ( 75 % ) showed bcl 2 positivity , 37 ( 90 % ) bax positivity , and 7 ( 17 % ) p 53 positivity . ^^^ CONCLUSIONS : We think that apoptosis related proteins such as Bcl 2 , Bax and p 53 may be significantly related to hormone function and local control in pituitary adenomas . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent studies on cells derived from mice deficient in both multi domain pro apoptotic genes of the Bcl 2 family , Bax and Bak , suggest that one or other of these proteins are required for the release of apoptogens such as cytochrome c from mitochondria . ^^^ In addition BH 3 only proteins of this family such as Bid are suggested to act as critical death inducing ligands via interactions with pro and anti apoptotic Bcl 2 family proteins with Bax or Bak at the mitochondrial surface . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proteasome inhibitors did not alter the levels of expression of the proapoptotic Bcl 2 family proteins , Bax and Bid , prior to the onset of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We previously reported that the leukemic cell death induced by an N terminally truncated Bax ( deltaN Bax : corresponding to amino acid 112 192 of full length Bax ) was not blocked by Bcl 2 or Bcl 10 ( L ) owing to the lack of the BH 3 domain needed to interact with the antiapoptotic Bcl 2 family molecules . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 expression is markedly increased in TGF beta 1 treated pre B cells , whereas cellular FLICE like inhibitory protein long ( c FLIPL ) , Bcl XL , Bax , and Bad expression remains unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The ratio of the anti apoptotic Bcl 2 to proapoptotic Bax expression increased , and expression of p 53 and interleukin 1beta converting enzyme activity decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study examines the effect of simultaneous targeting of COX 2 and PPARgamma on the proliferation of human breast cancer cells and on the expression of Bcl 2 , BAX , and caspases 3 and 9 , modulators of apoptotic cell death . ^^^ These effects were accompanied by upregulation of BAX and caspases 3 and 9 mRNA expression and downregulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : 56 adenomas ( < or = 10 mm ) from 39 patients were analysed for APC , CTNNB 1 and K ras mutations , allelic imbalance on 1p and 18q , microsatellite instability and immunohistochemical expression of HLA DR , BAX , BCL 2 and Ki 67 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined the impact of beta AR blockade with metoprolol on myocardial remodeling , apoptosis , pro apoptotic ( Fas , Fas ligand , Bax , and Bcl 10 ( S ) ) and anti apoptotic ( Bcl 10 ( L ) and Bcl 2 ) gene expression , and Bcl 10 ( L ) and Bcl 10 ( S ) protein in post infarction HF in rats . ^^^ Fas , Bax , and Bcl 2 mRNA expression was unchanged . ^^^ Metoprolol treatment resulted in : ( 1 ) improved LV remodeling ( P < 0 . 025 ) , ( 2 ) reduced myocardial apoptosis ( P < 0 . 005 ) , and ( 3 ) selective reduction in myocardial Bcl 10 ( S ) expression ( P < 0 . 001 ) without change in Fas , Fas ligand , Bax , Bcl 2 , or Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our findings suggest that this apoptotic cellular modulation is brought about by up regulation of the proapoptotic Bcl 2 family proteins Bax and Bim and down regulation of antiapoptotic Bcl 2 in mitochondrial fractions . ^^^ Additionally , ARF seems to down regulate Bcl 2 in a p 53 dependent manner while up regulating Bax / Bim via a p 53 independent pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of p 53 , p 21 , Bcl 2 , Bax , Bcl xL , and Bcl xS in the 22 HCC cases detected by western bioting was quantified with a densitometer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pituitary prolactin , growth hormone , Bcl 2 , Bax and PCNA concentrations were analysed by western blot analysis . ^^^ No change in the concentration of Bcl 2 or Bax , proteins implicated in apoptosis , was detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : We show that CD34+ AML fractions are more resistant to apoptosis than are corresponding CD 34 AML fractions , and that this is paralleled by higher Bcl 2 , Bcl xL , Mcl 1 , Pgp and lower Bax expression levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypoxia induced cell cycle arrest at the G0 / G1 phases and massive cell apoptosis after 24 hours through a reduction in the Bcl 2 to Bax ratio , downregulation of Bcl XL and Mcl 1 , and upregulation of caspase 3 and caspase 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , ethanol exposed cells display a blunting of TNF alpha induced Akt activation and Bcl 2 antagonist of cell death phosphorylation that may account , in part , for the increased sensitivity of the mitochondria to Bax mediated damage . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of bcl 2 , bax , and p 53 expression in diffuse large B cell lymphoma . ^^^ Because apoptosis related proteins may play an important role in predicting the prognosis of DLCL , the current study investigated the prognostic significance of a high level of bcl 2 , bax , and p 53 expression in relation to clinical characteristics in patients with DLCL . ^^^ Paraffin embedded specimens from 94 patients with de novo DLCL were analyzed immunohistochemically for bcl 2 , bax , and p 53 gene expression . ^^^ The responses to therapy and survival were then analyzed in 94 uniformly staged patients . bcl 2 expression was identified in 24 patients ( 26 . 4 % ) , bax expression in 35 patients ( 37 . 6 % ) , and p 53 expression in 21 patients ( 22 . 6 % ) . bax expression proved to be a statistically significant prognostic factor in predicting the overall survival ( OS ) ( P = 0 . 0015 ) and disease free survival ( DFS ) ( P = 0 . 0052 ) , regardless of other clinical factors or immunohistological results . ^^^ A multivariate analysis revealed that IPI score , bulky mass , and bax expression were all significantly associated with the DFS or OS . bax and bcl 2 should be considered as independent biologic prognostic parameters in DLCL , thereby aiding in the identification of patient risk groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relationship between expression of Bax and Bcl 2 proteins and apoptosis in radiation compound wound healing of rats . ^^^ OBJECTIVE : To study the relationship between the expression of Bax , Bcl 2 proteins , and apoptosis in radiation compound wound healing of rats . ^^^ METHODS : Apoptosis , Bax and Bcl 2 proteins were estimated by in situ terminal labeling ( TUNEL ) and immunohistochemical methods . ^^^ CONCLUSIONS : Bax and Bcl 2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transient transfection of cells with antisense oligonucleotides to Bax or transient overexpression of Bcl 2 prevented VES induced mitochondrial permeability transition and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , the proteins of the p 53 regulated genes , p 21 ( WAF 1 ) and Bax , were increased with a similar time , while Bcl 2 and Bcl 10 ( L ) expression was lowered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study investigated expression levels of the anti apoptotic proteins BCL 2 , BCL XL and MCL 1 and the pro apoptotic proteins BAX and BCL XS in a series of 112 peripheral T cell lymphomas ( PTCLs ) classified according to the WHO classification . ^^^ Using immunohistochemical methods and a 10 % cut off , each protein was detected in a subset of PTCLs : BCL 2 in 46 % , BCL XS in 49 % , BAX in 57 % , BCL XL in 57 % , and MCL 1 in 65 % . ^^^ The proliferation index , assessed by the MIB 1 antibody , correlated with expression levels of MCL 1 ( R = 0 . 42 , p = 0 . 003 ) , BCL 2 ( R = 0 . 32 , p = 0 . 027 ) , BAX ( R = 0 . 33 , p = 0 . 014 ) , and BCL XL ( R = 0 . 34 , p = 0 . 015 ) ( Spearman rank ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study examined cutaneous T cell lymphoma ( CTCL ) cell lines and cutaneous lesions for the presence of Bcl 2 gene family members and found that the two apoptosis inhibiting members Bcl xL and Mcl 1 and the two apoptosis supporting members Bad and Bax were expressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The single knockout of Bax or Bak ( but not Bid ) or the transfection enforced expression of mitochondrion targeted ( but not endoplasmic reticulum targeted ) Bcl 2 conferred protection against CPX ( but not NFX * ) induced MMP and death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objective was to evaluate the immunohistochemical expression of p 53 , p 21 , bax , bak , fas , bcl 2 and bcl 10 proteins in 10 endometriomas , 20 benign ovarian tumours ( 10 mucinous , 10 serous ) and 30 malignant ovarian tumours ( 9 mucinous , 19 serous ; 2 endometrioids ) . p 53 positive cells ( mean+ / SD ) in endometriomas , and benign and malignant tumours were 1 . 9+ / 3 . 2 , 0 and 16 . 2+ / 33 . 0 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The detected pattern of the p53 / bcl 2 ratio in HAK suggests an important role for another gene : the proapoptotic gene bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relationship between p 53 , bax and bcl 2 expression and cell apoptosis in intrahepatic cholestasis of pregnancy ] . ^^^ OBJECTIVE : To investigate the changes of gene p 53 , bax and bcl 2 expression and to study the role on cell apoptosis in placenta through apoptosis in placenta with intrahepatic cholestasis of pregnancy ( ICP ) . ^^^ METHODS : TdT mediated dUTP nick end labeling ( TUNEL ) and immuno histochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index and the expression of p 53 , bax and bcl 2 in placenta tissues . ^^^ The expression of bcl 2 of these positions in ICP group was lower than that in control group ( P < 0 . 01 ) p 53 , bax and bcl 2 expressions in ( chi + / s ) % in the syncytoitrophoblast cells of placenta with ICP is 75 . 9 + / 8 . 2 , 65 . 8 + / 13 . 8 , 45 . 3 + / 11 . 3 . ^^^ Abnormal overexpression of p 53 , bax and the low expression of bcl 2 on placental tissue of ICP was the main reason of placental apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Using TdT mediated dUTP biotin nick end labeling ( TUNEL ) for cell apoptosis and immunohistochemical staining for proliferating cell nuclear antigen ( PCNA ) , Bcl 2 and Bax protein expression . ^^^ RESULTS : In SCC of tongue , the positive rates of apoptosis index ( AI ) , Bcl 2 , Bax and PCNA were 55 . 8 % , 65 . 4 % , 73 . 1 % and 100 . 0 % respectively . ^^^ In the univariate Kaplan Meier analysis , low expression of Bax , high expression of PCNA , Bcl 2 / Bax expression ratio > 1 and high staging were all associated with lower survival rate ( Log rank test , P < 0 . 05 , respectively ) , AI was only correlated with grading . ^^^ CONCLUSIONS : The levels of Bax and PCNA expression and Bcl 2 / Bax expression ratio have independent prognosis value in squamous cell carcinoma of the tongue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Lymphoma development in Bax transgenic mice is inhibited by Bcl 2 and associated with chromosomal instability . ^^^ Bax is a Bcl 2 family member that promotes apoptosis but has paradoxical effects on lymphoma development in p 53 deficient mice . ^^^ To better understand the mechanism of Bax induced lymphoma development , the effect of Bax levels , p 53 status and Bcl 2 coexpression on lymphoma development were determined . ^^^ Importantly , coexpression of Bcl 2 delayed lymphoma development in Lck Bax transgenic mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further Western blot analysis showed PIF increased the cytosolic content of cytochrome c , as well as expression of the pro apoptotic protein bax but not the anti apoptotic protein bcl 2 , which were both attenuated by 50 microM EPA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was revealed that LIGHT treatment resulted in down regulation of anti apoptosis Bcl 2 family member : Bcl 2 , Bcl 10 ( L ) , Bag 1 , and Mcl 1 ; up regulation of pro apoptosis Bcl 2 family member : Bak and Ser ( 112 ) phosphor Bad ; down regulation of pro apoptosis Bcl 2 member Bax ; the other pro apoptosis member Bid remains unaltered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression and distribution of apoptotic gene Bcl 2 and Bax on olfactory of rat , and its relation with the FGF and to investigate the mechanism of senile dysosmia . ^^^ The positive expression rate of Bax in senile rats was a little stronger than that in young rats , but the differences were not statistically significant . ( P > 0 . 05 ) CONCLUSION : The expression of FGF and anti apoptotic gene Bcl 2 on olfactory bulb decrease with the increase of age . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The proliferation cell nuclear antigen ( PCNA ) , apoptosis associated gene protein ( Bcl 2 , Bax ) were determined in 26 tissue samples of nasal polyps and 14 controls from normal inferior turbinates respectively . ^^^ RESULTS : ( 1 ) The positive expression e of PCNA , Bcl 2 and Bax were significantly higher in epithelium of nasal polyps than the controls , and the expression rate of Bcl 2 / Bax , however , tended equipoise . ( 2 ) The expression of Bcl 2 were significantly stronger than Bax in glands , eosinophils of nasal polyps tissue , and there were not significant differences in inferior turbinates . ^^^ CONCLUSION : There is strongly proliferation activity in the epithelium of nasal polyps , and expression imbalance of Bcl 2 / Bax may be one of the important factors of eosinophilia in nasal polyps tissue . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bcl 2 and Bax protein were detected by immunohistochemical method . ^^^ The level of Bcl 2 protein and ratio of Bcl 2 to Bax protein of HCC cells were significantly lower in Groups A , B , C and D than those in the non TACE group ( P < 0 . 05 ) . ^^^ CONCLUSION : Preoperative TACE regimens may enhance apoptosis of HCC cells by up regulating the expression of Bax protein and down regulating the expression of Bcl 2 protein and ratio of Bcl 2 to Bax protein expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IGFBP 5 induced apoptosis was associated with a transcriptional increase in expression of the proapoptotic regulator bax and decrease in the anti apoptotic bcl 2 compared with vector controls . ^^^ IGFBP 5 expressing tumors also had a significantly elevated level of bax mRNA and decreased levels of bcl 2 mRNA compared with vector tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of anti apoptotic members of the Bcl 2 family such as Bcl 2 and Bcl XL has been implicated in cancer chemoresistance , whereas high levels of pro apoptotic proteins such as Bax promote apoptosis and sensitize tumor cells to various anticancer therapies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was not accompanied by increased ara CTP formation , DNA incorporation of [ 3H ] ara C , or altered expression of Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 and BAX expression and cell proliferation , after partial hepatectomy with and without ischemia , on cholestatic liver in rats : an experimental study . ^^^ This study investigates the expression apoptosis associated genes bcl 2 and bax , and the presence of apoptosis and cell proliferation after PHx , in normal and jaundiced rats with or without superimposed ischemia . ^^^ Liver tissue was obtained and pathologic examination included : ( a ) H & E stain , ( b ) in situ hybridization ( detection of bcl 2 and bax mRNA ) in paraffin sections , ( c ) Western blot analysis for the evaluation of bcl 2 and bax protein levels , ( d ) in situ hybridization ( TUNEL ) for the detection of apoptotic bodies , and ( e ) immunohistochemical stains ( streptavidin biotin method ) in paraffin sections to detect cells that ( 1 ) express bcl 2 and bax proteins and ( 2 ) undergo proliferation ( Ki67+ cells ) . ^^^ After hepatectomy , there was an early decrease in the protein and mRNA levels of antiapoptotic gene bcl 2 and a late increase of proapoptotic gene bax and the ABI , compared to controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DNA ladders were measured to evaluate adipocyte apoptosis , and RT PCR was used to analyze the expression of bcl 2 and bax gene . ^^^ RESULTS : ( 1 ) Central administration of Y 5 receptor antisense ODN significantly decreased body weight , fat pads , and average adipocyte area . ( 2 ) DNA fragmentation was presented after electrophoresis at both epididymal and retroperitoneal adipose tissue . ( 3 ) The expression of bcl 2 gene was downregulated , while the expression of bax was upregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Different expression patterns of Bcl 2 , Bcl xl , and Bax proteins after sublethal forebrain ischemia in C57Black / Crj6 mouse striatum . ^^^ Although changes in the expression of apoptosis related proteins ( Bcl 2 , Bcl xl , and Bax ) have been considered to be crucially important in ischemic injury , the roles these proteins play in ischemic preconditioning induced by sublethal forebrain ischemia have not been elucidated . ^^^ Therefore , we investigated the transcription and expression of Bcl 2 , Bcl xl , and Bax in striatum of mice subjected to sublethal forebrain ischemia and lethal ischemia , with or without ischemic preconditioning . ^^^ CONCLUSIONS : Upregulation of Bcl 2 and Bcl xl but not Bax may have a role in protective ischemic preconditioning . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Fas and Bcl 2 family genes ( Bax , Bcl 2 , Bcl xL , Bcl xS ) were not affected by NS 398 treatment in all three cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Fas antigen , p 21 , p 53 , bax and bcl 2 related to radiation induced apoptosis was comparable to that of the caffeine untreated group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The compound activated caspase 1 and 3 ( but not caspase 8 ) and increased the ratio of bax to bcl 2 mRNA via reduction of bcl 2 mRNA expression in the same cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also analyzed the relationship between TCF 4 gene splicing isoforms , proliferation ( proliferating cell nuclear antigen labeling index ) , and apoptosis [ antiapoptotic factors ( Bcl 2 and Bcl 10 ( L ) ) , proapoptotic factors ( Bak and Bax ) , and caspase 3 ] in RCC samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ras , Bcl 2 and Bax were quantified by Western blotting . ^^^ These effects were associated with changes in cell cycle distribution , induction of DNA fragmentation and a decrease in the Bcl 2 to Bax ratio , which are features of apoptotic cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PURPOSE : We hypothesized that apoptosis is a downstream event in erectile dysfunction , and pro apoptotic ( Bak and Bax ) and anti apoptotic ( Bcl 2 and Bcl 10 ) factors are involved in the etiology of diabetes induced erectile dysfunction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the process , the expression of COX 2 mRNA and protein and Bcl 2 protein was down regulated , while that of Bax and Bak proteins was up regulated , and the activity of caspase 3 was elevated . ^^^ CONCLUSION : TGZ inhibits cell proliferation and induces apoptosis in HepG 2 cells , which may be associated with the activation of caspase 3 like proteases , down regulation of the expression of COX 2 mRNA and protein , Bcl 2 protein , the elevation of PGE 2 levels , and up regulation of the expressions of Bax and Bak proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to investigate pathways of apoptosis in mouse uterine epithelium and decidua during early pregnancy ( day 4 . 5 7 . 0 post coitum ) , we have investigated different proteins such as TNFalpha , TNF receptor 1 , Fas ligand , Fas receptor 1 , Bax and Bcl 2 as well as caspase 9 and caspase 3 using immunohistochemistry . ^^^ Bax and Bcl 2 revealed a complementary expression pattern with Bax in the primary and Bcl 2 in the adjacent decidual zone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR and Western blot analyses showed that immobilization stress increased and decreased the expression of pro apoptotic gene bax and anti apoptotic bcl 2 genes , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Temporal alterations in cellular Bax : Bcl 2 ratio following traumatic brain injury in the rat . ^^^ Using immunohistochemistry , Western analysis and in situ hybridization , the expression of the anti cell death protein , Bcl 2 , and the pro cell death protein , Bax , was evaluated following lateral fluid percussion ( FP ) brain injury of moderate severity ( 2 . 3 2 . 6 atm ) in adult male Sprague Dawley rats . ^^^ By 2 h post injury , a marked reduction of cellular Bcl 2 immunoreactivity ( IR ) and a mild decrease in cellular Bax IR were observed in the temporal and occipital cortices , and in the hippocampal CA 3 ipsilateral to the site of impact . ^^^ These decreases in Bcl 2 and Bax IR appeared to precede the overt cell loss in these regions that was evident at 24 h . ^^^ Immunoblot analysis supported the immunohistochemical data , with a modest but significant reduction in the intensities of both the Bcl 2 and Bax protein bands at 2 h ( p < 0 . 05 compared to sham levels ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results indicate that AM protects against myocardial infarction , arrhythmia , and apoptosis in I / R injury via suppression of oxidative stress induced Bax and p 38 MAPK phosphorylation and activation of the Akt Bad Bcl 2 signaling pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax proteins were estimated by western immunoblotting method . ^^^ The levels of Bcl 2 and Bax remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In both cases , Bcl 2 expression was consistently down regulated , whereas levels of other members of the Bcl 2 family , such as Bax and Bcl 10 , did not change in paclitaxel resistant cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis also showed an increased Bax : Bcl 2 ratio , indicating the induction of the mitochondria mediated cell death pathway in the lesion and penumbra . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The expressions of apoptosis associated genes ( bcl 2 , bax , bcl 10 ( L ) and bcl 10 ( S ) ) and the activity of caspase 3 were studied by reverse transcription polymerase chain reaction ( RT PCR ) and western blot in the cisplatin resistant ( A2780 / DDP , COC1 / DDP ) and sensitive human ovarian cancer cells ( A 2780 and COC 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in expression of apoptosis associated ( Bcl 2 and Bax ) and regeneration associated ( GAP 43 ) proteins . ^^^ The expression of Bcl 2 and Bax proteins was studied with immunohistochemistry , immuoblotting and RT PCR in the uterine horn and uterine cervix projecting neurons of the inferior mesenteric ganglion ( IMG ) of the sexually immature gilts after partial or total hysterectomy . ^^^ The weak immunoreactivity to Bcl 2 and GAP 43 and moderately intense immunoreactivity to Bax was revealed in all FB+ ( FB+ ) neurons of control and hysterectomized pigs . ^^^ No difference in the intensity of immunostaining for Bcl 2 , Bax and GAP 43 was found between control and hysterectomized gilts . ^^^ Immunoblotting revealed the presence of Bcl 2 and Bax proteins in IMGs of control and hysterectomized animals and no difference in the band intensities between control and experimental groups was detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When compared with undifferentiated controls , ATRA induced Bcl 2 expression , while loss of Bax expression was observed only in cells differentiated by ATRA + BMP 6 . ^^^ The high Bcl 2 / Bax ratio in cells differentiated by ATRA and ATRA + BMP 6 , respectively , correlated with the survival of these cells in serum free media . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we found that HDB induced apoptotic cell death was accompanied by upregulation of cyclin D 3 , Bax , and p 21 and down regulation of Bcl 10 ( L ) , while HDB had no effect on the levels of Bcl 2 and Bad protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
D 3 induced apoptosis of retinoblastoma cells is associated with reciprocal changes of Bcl 2 and bax . ^^^ Apoptosis of Y 79 cells in response to 1 , 25 ( OH ) ( 2 ) D ( 3 ) was demonstrated by the means of TdT dUTP terminal nick end labelling ( TUNEL ) , annexin 5 staining , and detection of DNA fragmentation on agarose gels . 1 , 25 ( OH ) ( 2 ) D ( 3 ) induced programmed death of Y 79 cells was accompanied by a concentration dependent increase in Bax protein and a reduction in Bcl 2 content . ^^^ These findings suggest that 1 , 25 ( OH ) ( 2 ) D ( 3 ) inhibits the growth of retinoblastoma cells by causing cell cycle arrest and apoptosis . 1 , 25 ( OH ) ( 2 ) D ( 3 ) induced programmed death of retinoblastoma cells appears to involve reciprocal changes in Bcl 2 and Bax proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Abeta peptides , used at concentrations able to induce oxidative stress , elicit a classic type of neuronal apoptosis involving mitochondrial regulatory proteins and pathways ( i . e . affecting Bax and Bcl 2 protein levels as well as release of cytochrome c in the cytosol ) , poly ADP rybose polymerase cleavage and activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Four hours after 30 minutes of incomplete cerebral ischemia , the brains were removed for determination of the expression of the apoptosis regulating proteins Bax , Bcl 2 , p 53 , and Mdm 2 using immunofluorescence and Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To confirm inhibition of apoptosis with ATA , densitometrically , Bcl 2 ( % of normal myocardium ) was significantly increased vs . control ( 102+ / 12 * vs . 68+ / 9 ) and Bax as well as the activated caspase 3 were significantly reduced vs . control ( 108+ / 17 * vs . 194+ / 42 and 29+ / 4 * vs . 174+ / 43 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In these conditions , microscopic analysis revealed chromatin condensation in dying cells and the Bcl 2 , Bcl 10 ( L ) / Bax mRNA balance was altered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : In order to study the ki 67 , bcl 2 , bax proteins expression and significance in the benign and malignant epithelium of the larynx . ^^^ METHOD : An immunohistochemical S P assay was used to detect the expression of ki 67 , bcl 2 , bax in LSCCs , epithelial dysplasia , papillomas , simple hyperplasia and normal laryngeal epithelium . ^^^ Ki 67 , bax , Bcl 2 could help in estimation the premalignant stages of the larynx . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Adp14 / p53 treatment leads to an elevated bax : bcl 2 ratio and induction of apoptosis in vitro and in vivo , coupled with a failure of the tumor cells to induce neovascularization in vivo . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that I3C inhibited the growth of breast cancer cells and induced apoptosis in these cells , concomitant with upregulation of Bax , and downregulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Remarkably , these events occurred in the absence of any reduction in the expression of the Bcl 2 family members Bcl 2 , Mcl 1 , and Bcl xL or any change in the proapoptotic molecules Bad or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of survivin , Fas , bcl 2 and bax in bone marrow cells from acute myeloid leukemia patients and its clinical significance ] . ^^^ To study the clinical significance of the expression of antiapoptosis gene , survivin and bcl 2 , and proapoptosis gene , Fas and bax , in acute myeloid leukemia ( AML ) , RT PCR was used to examine the expression of survivin and flow cytometry ( FCM ) to detect the expression of Fas , bcl 2 , bax and bcl 2 / bax ratio in 68 cases of AML . ^^^ The survivin positive NR cases showed a significantly decreased Fas and increased bcl 2 expression as compared with pretreatment expression ( P < 0 . 001 ) . bcl 2 / bax ratio was also significantly higher in NR cases . ^^^ In conclusion : 70 . 6 % AML cases showed positive for survivin expression with a lower CR rate , the survivin positive AML showed a low Fas with high bcl 2 expression and bcl 2 / bax ratio as compared to the survivin negative cases . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to study the effects of losartan on cardiomyocyte apoptosis following ischemia ( 0 . 5 h ) and reperfusion ( 48 h ) in vivo and bcl 2 and bax gene expression , TUNEL staining method , immunohistochemistry and in situ hybridization histochemistry ( ISHH ) were used to monitor the apoptotic cells , mRNA and protein of gene expression , respectively . ^^^ Bcl 2 / bax ratio was 1 . 413 ( control group ) , 1 . 376 ( sham operation group ) and 2 . 016 ( losartan treated group ) respectively . ^^^ The mechanism might be that bax gene expression was inhibited to increase bcl 2 / bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The treatment of these cells with anti Id resulted in no change in Fas ligand and Bax expression , but the expression of Bcl 2 was decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cells overexpressing cyclin E display a marked decrease in the expression of Bcl 2 , an antiapoptotic protein , and increased levels of the proapoptotic proteins Bad and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , all four proteins with caspases 3 , 9 , Bcl 2 and Bax were highly expressed between embryonic day 19 and 1 week age of early postnatal development , but thereafter the expression dramatically declined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of cell death by the BH 3 only Bcl 2 homolog Nbk / Bik is mediated by an entirely Bax dependent mitochondrial pathway . ^^^ Immunoprecipitation studies revealed that Nbk interacts with Bcl 10 ( L ) and Bcl 2 but not with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was reported that SCF / c kit , Bcl 2 and Bcl xl inhibited the apoptosis while caspase 3 , Fas , Bax and clusterine stimulated it . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study the alteration of bax , bcl 2 , p 170 expressing and the second effect on the adriamycin ( ADR ) induced cytotoxicity in acquired tamoxifen ( TAM ) resistant MCF 7 cell line . ^^^ METHODS : The bax , bcl 2 , p 170 protein expressions were detected by flow cytometry respectively , as well as the content of intracellular ADR . ^^^ RESULTS : The percentages of bax , bcl 2 expressions in the acquired TAM resistant MCF 7 were down regulated from 53 . 17+ / 1 . 45 % to 28 . 70+ / 1 . 41 % ( P < 0 . 01 ) , 41 . 53+ / 2 . 17 % to 37 . 87+ / 1 . 86 % P > 0 . 05 respectively , the p 170 was up regulated from 27 . 43+ / 2 . 16 % to 32 . 13+ / 1 . 31 % ( P < 0 . 05 ) , after a 16 week treatment with 1 10 10 ( 7 ) mol / L TAM . ^^^ CONCLUSION : The acquired TAM resistant MCF 7 accompanied with a declined ADR cytotoxicity , down regulation of bcl 2 / bax induced apoptosis and up regulation of p 170 induced drug efflux may had a synergic devotion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of either phosphatidylinositol 3 kinase or AKT led to a decreased protein level of the antiapoptotic gene BCL 2 and an increased protein level of the proapoptotic gene BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlation with bax and bcl 2 expression , microsatellite instability status , and outcome . ^^^ Results were compared with clinicopathologic factors , bcl 2 and bax expression , microsatellite instability ( MSI ) status , and prognosis . ^^^ Increased expression of IGF IR and bcl 2 ( > 50 % cells ) was seen in 60 cases ( 67 % ) and 15 cases ( 17 % ) , respectively ; loss of PTEN was seen in 13 cases ( 15 % ) and of bax in 11 cases ( 12 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By its mechanism of inhibiting protein degradation , PS 341 targets a wide range of pathways that are relevant to tumor progression and therapy resistance , and can directly modulate expression of cyclins , p 27 ( Kip 1 ) , p 53 , NF kappaB , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
G / GO induced apoptosis determined by poly ( ADP ribose ) polymerase cleavage , an increase in expression of p 53 and Bax , and a decrease in Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , no significant change in mitochondrial membrane potential and expression of Bax and Bcl 2 was observed . ^^^ These results indicate that hyperthermia and AAPH induce enhanced apoptosis and subsequent cell killing via two pathways ; a pathway dependenton increase in LPO and [ Ca2+ ] 1 , and a pathway associated with cytochrome c release and subsequent caspase activation without changes of mitochondrial membrane potential and Bax / Bcl 2 expression in these cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A decrease in the anti apoptotic protein , Mcl 1 , was detected in QUE treated HL 60 cells , whereas other Bcl 2 family proteins including Bax , Bcl 2 , Bcl XL , and Bag remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The tumors ' location , size , histologic characteristics , immunophenotype , proliferative activity index ( assessed by proliferating cell nuclear antigen ( PCNA ) and Ki 67 immunoreactivity ) and the apoptotic markers bcl 2 and bax , were considered as potential prognostic factors and were correlated with patient survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Brain damage was measured 22 days after injury . bcl 2 , bax , and cytochrome c where measured by Western blot 24 h after injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Formalin fixed and paraffin embedded tissues from 25 samples of lentigo maligna , 20 samples of solar keratoses , and 5 samples each of normal sun exposed and non sun exposed skin ( controls ) were immunostained with antibodies directed against the proapoptotic markers bax and p 53 , the antiapoptotic marker bcl 2 , and the proliferation marker ki 67 . ^^^ Eight percent of the lentigo maligna samples were positive for keratinocyte expression of bcl 2 , 24 % were positive for p 53 , and 76 % were positive for bax ; respective findings for solar keratoses were 35 % , 85 % , and 90 % . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIMS : To correlate the expression of a series of apoptotic and oncogene markers ( including p 53 , Bcl 2 , BAX , Bcl XL , p21WAF , 1 / CIP1 , cyclin D 1 , HER 2 / neu ) in thymic epithelial tumours with histological type , stage and resectability and to determine whether the information on HER 2 / neu would be valuable in identifying patients who are eligible for anti HER 2 / neu treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , treatment of cells with LY 294002 , to inhibit phosphoinositide 3 kinase ( PI3K ) , caused downregulation of Bcl 2 and Mcl 1 and allowed deltaMEKK 1 : ER * to elicit a robust apoptotic response characterized by activation of Bax and caspases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The study of molecular biological markers for prediction of recurrence free survival in breast cancer stage 1 IIA demonstrates that high expression of thymidilate synthetase and high maximal density of microvessels are prognostically effective and that the prognosis is influenced by expression of both Bcl 2 and Bax . ^^^ Low recurrence free survival was observed in Bcl 2 / Bax patients ( 31 % , median 44 months ) while such survival was high in Bcl 2+ / Bax patients ( 86 % , median was not reached ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell death , Bcl 2 , Bax , and the cerebellum . ^^^ Bax and Bcl 2 are prototypical members of a large family of Bcl 2 like proteins that play a key role in regulating programmed cell death in many cell types . ^^^ The purpose of this review is to summarize recent findings about the role of Bcl 2 and Bax in regulating programmed cell death in the cerebellum during normal development and in a mouse model of cell autonomous and target related cell death , the Lurcher mutant . ^^^ Both Bcl 2 and Bax are expressed in the developing cerebellum and recent studies of Bcl 2 transgenic mice and Bcl 2 or Bax knock out mutants have shown that both proteins are likely to play a role in regulating cell death among Purkinje cells , granule cells and olivary neurons . ^^^ For example , the number of Purkinje cells is increased by over 30 % in one line of Bcl 2 overexpressing transgenics and in Bax knock out mutants , suggesting that both proteins may be involved in naturally occurring Purkinje cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , it down regulated the expression of Bcl 2 and poly ( ADP ribose ) polymerase , but up regulated the expression of caspase 3 and Bax in dopaminergic ( SK N SH ) neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate molecular events in the apoptosis , protein expressions of Bcl 2 , Bax , Fas , and FasL were measured by Western blotting using specific antibodies in HL 60 cells . ^^^ The level of Bcl 2 remained largely unchanged , but the Bax , Fas , and FasL expression showed up regulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interferon alpha induced apoptosis in U 266 cells is associated with activation of the proapoptotic Bcl 2 family members Bak and Bax . ^^^ Here , we show that both proapoptotic Bcl 2 family members Bak and Bax were activated by IFNalpha , strictly in correlation with the induction of apoptosis . ^^^ Overexpression of Bcl 2 blocked apoptosis induced by IFNalpha totally abolished Bak activation , as well as decreased the amount of activated Bax . ^^^ We conclude that IFNalpha induces Bak and Bax activation via distinct mechanisms involving an unknown protease , and that their activation is regulated by Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
STUDY DESIGN : Tissue sections from 60 NSCLCs were assessed for the expression of p 53 , Ki 67 , bcl 2 , bax and FasL using immunohistochemistry and antibodies . ^^^ Bcl 2 and bax quantified immunostaining demonstrated a weak negative correlation with shape factor values ( P = . 039 and P = . 025 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : After a 4 day incubation with various concentrations of AGE ( 8 , 32 , 125 , 500 , 2 , 000 mg / L ) which were prepared in vitro by incubating bovine serum albumin ( BSA ) with glucose , we studied the degree of apoptosis and the expressions of apoptotic genes ( Bax and bcl 2 ) in BRPs by staining with the Annexin 5 FITC and propidium iodide ( PI ) , terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) and immunohistochemistry . ^^^ CONCLUSIONS : AGE can induce a significant apoptosis of BRPs in a dose dependent manner and the rate of apoptosis was determined by the Bax / bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic bcl 2 family members , Bax and Bak , are essential for developmental photoreceptor apoptosis . ^^^ The purpose of this study was to characterize the roles in retinal development of the proapoptotic Bcl 2 family members Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the anti apoptosis proteins , Bcl 2 and Mcl 1 expression decreased time dependently while Bax expression increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of these cells caused by Phx was unlikely to be associated with p 53 , Bax , and Bcl 2 , because the levels of these proteins were not altered regardless of the presence or absence of Phx . ^^^ The present results suggest that Phx demonstrates antitumor activity against human endometrial adenocarcinoma cell lines EN and KLE cells , by inducing both cell cycle accumulation at S and G2M and apoptosis associated with p 53 , Bcl 2 and Bax insensitive pathways . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine what factors can precisely predict the radio sensitivity or radio resistance of early stage laryngeal squamous cell carcinomas ( LSCC ) , protein expression of p 53 , Bcl 2 and Bax and loss of heterozygosity ( LOH ) at 3p and 9p loci were investigated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , up regulation of Bax and down regulation of Bcl 2 was also observed indicating that these two apoptotic regulators may play important roles in terazosin mediated cell death pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR demonstrated that the pro apoptic gene bax was induced while the anti apoptic gene bcl 2 and cell cycle active gene PCNA were reduced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immediately after isolation , there was no difference in Bcl 2 or bax expression among the four different samples , or in Fas and FasL mRNA . ^^^ However , when cultured , lymphocytes treated with ECP showed a rapid downregulation of Bcl 2 , an upregulation of bax with an increased bax / Bcl 2 ratio , a decrease in bcl 2 mRNA and an increase in Fas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of survivin gene and its relation with the expression of bcl 2 and bax protein in epithelial ovarian cancer ] . ^^^ OBJECTIVE : To study the expression of apoptosis related gene survivin and its relation with expression of bcl 2 , bax protein in epithelial ovarian cancer . ^^^ Expression of bcl 2 and bax was detected by immunohistochemistry streptomycin avidin biotin peroxidase complex ( SABC ) method , and correlation between them was analyzed . ^^^ Expression of bcl 2 and bax protein was positively and negatively correlated with expression of survivin gene respectively . ^^^ CONCLUSION : survivin gene may play an important role in pathogenesis of ovarian cancer , apoptosis related gene bcl 2 may have a synergic role and bax have an antagonistic role with survivin gene in formation and progression of ovarian cancer . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no difference found in the expression of proapoptotic ( Bax and Bcl 10 ( s ) ) or antiapoptotic ( Bcl 2 and Bcl 10 ( L ) ) genes nor in the expression of the tumor suppressor gene p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression and function of apoptosis related genes Bcl 2 / Bax and Fas / Fas L in the course of stress ulcer ] . ^^^ OBJECTIVE : To investigate the correlation between the expressions of apoptosis promoting gene Bax , apotosis inhibiting gene Bcl 2 , and Fas / FasL in gastric mucosa in the course of stress ulcer . ^^^ The expression of Bcl 2 / Bax protein was examined by immunohistochemical assay . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ZD 1839 alone induced accumulation of cells in the G0 / G1 phase of the cell cycle at 24 h accompanied by a concomitant increase in p 21 , p 27 and Bax , a significant decrease in Bcl 2 and a decrease in Akt phosphorylation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in the expression of caspase 3 , bax , bcl 2 and p21cip / waf were assessed by quantitative Western blotting . ^^^ The combination effectively up regulated caspase 3 and bax and down regulated bcl 2 and p21cip / waf . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : With the methods of in situ hybridization and immunohistochemistry staining , PKC and apoptosis gene ( Bcl 2 , Bax ) expression were measured between nasal polyps from 26 patients and inferior turbinate mucous membrane tissue ( ITMMT ) from 20 normal persons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , EGCG had a concurrent effect on two important transcription factors p 53 and NF kappaB , causing a change in the ratio of Bax / Bcl 2 in a manner that favors apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of BclXS protein was not detected by conventional Western blotting and the expression of Bcl 2 and Bax protein was not altered by wortmannin in all three cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Likewise , protein expression levels of Bcl 2 and Bax genes in these tumors , determined by Western blot analysis , showed no treatment related stimulation of apoptotic process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The analysis of the bcl 2 family of proteins demonstrates that bcl 2 , bax and bcl XL are involved in triggering apoptosis in spheroids exposed to 5 Gy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that codeinone enhanced the Pro apoptotic Bax protein expression , but reduced the anti apoptotic Bcl 2 protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , EGCG increased Bcl 2 expression but decreased Bax expression , causing an increase in the Bcl 2 to Bax ratio . ^^^ In conclusion , this study demonstrates that EGCG promotes keratinocyte survival and inhibits the UV induced apoptosis via two mechanisms : by phosphorylating Ser 112 and Ser 136 of Bad protein through Erk and Akt pathways , respectively , and by increasing the Bcl 2 to Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of B cell leukaemia / lymphoma 2 ( Bcl 2 ) , but not those of Bcl 2 associated 10 protein ( Bax ) or Bcl 10 ( L ) , were down regulated concentration dependently by magnolol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro apoptotic activity of transiently expressed BCL 2 occurs independent of BAX and BAK . ^^^ Overexpression of BCL 2 in a Bax null human colon cancer cell line ( HCT116Bax / ) induces efficient cell death . ^^^ The pro apoptotic activity of BCL 2 is also observed in a Bax null cells in which BAK expression is inhibited by stable RNAi expression . ^^^ Our results suggest that BCL 2 contains an intrinsic pro apoptotic activity and can induce apoptosis independent of BAX and BAK under specific conditions . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further understand such merosin driven survival signaling , we analyzed the expression of five Bcl 2 homologs ( Bcl 2 , Bcl 10 ( L ) , Bax , Bak , Bad ) and one non homologous associated molecule ( Bag 1 ) in normal and merosin deficient myotubes , with or without pharmacological inhibitors for Fyn and p 38 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In situ hybridization and RT PCR were used to detect the expression level of bcl 2 and bax . ^^^ Through the methods of in situ hybridization and RT PCR , we found the expression of bcl 2 increased whereas bax decreased significantly in the hypoxic group . ^^^ CONCLUSION : The alternation in bcl 2 and bax expression induced by hypoxia play an important role in the pulmonary artery remodeling which is the main pathologic change of pulmonary hypertension secondary to hypoxia . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MEASUREMENTS : Clinical and serologic characteristics ; liver biopsy ; bcl 2 rearrangement , Bcl 2 expression , and the ratio of Bcl 2 to Bax in total peripheral blood mononuclear cells and cell subgroups ; and sequence analysis of the junction of bcl 2 and IgH joining segments in positive samples . ^^^ Overexpression of Bcl 2 protein and a high ratio of Bcl 2 to Bax were observed in samples from patients with bcl 2 rearrangement . ^^^ The high ratio of Bcl 2 to Bax in patients with bcl 2 rearrangement and disappearance of the rearrangement with antiviral therapy suggest that the translocation is associated with the antiapoptotic function of Bcl 2 and that HCV infection is linked to inhibition of B cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , DOX treatment also upregulated Bax and downregulated Bcl 2 levels in the cells . ^^^ The catalase treatment however only suppressed DOX induced Bax upregulation but had no effect on Bcl 2 downregulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A pro apoptotic protein Bax is a Bcl 2 family member and forms homodimers and also heterodimerizes with death antagonists , Bcl 2 and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of myocardial Bcl 2 and Bax was increased in CR compared with both NL ( Bax , 4 . 3 fold ; P < 0 . 01 ; Bcl 2 , 5 . 9 fold ; P < 0 . 01 ) and IsCM ( IsCM : Bax , 2 . 2 fold ; P < 0 . 05 ; Bcl 2 , 3 . 2 fold ; P < 0 . 01 ) groups . ^^^ The rate of apoptosis and the Bcl 2 / Bax ratio independently correlated to graft survival in CR ( activation of caspase 9 : r = 0 . 87 ; P < 0 . 01 ; Bcl 2 / Bax : r = 0 . 57 ; P = 0 . 05 ) . ^^^ Compared with native atherosclerosis , coronary arteries with CAV showed more medial apoptosis ( 7 . 8 fold ; P < 0 . 01 ) and higher Bcl 2 levels ( 5 . 1 fold ; P < 0 . 01 ) with lower Bax levels ( threefold ; P < 0 . 05 ) in the intima . ^^^ These results indicate that abnormal Bcl 2 and Bax expression in myocardium and coronary arteries of cardiac allografts with CR is distinct from that in IsCM and suggest that balancing Bcl 2 to Bax in transplanted hearts promotes long term graft survival . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because there are multiple members of the Bcl 2 family ( 24 members to date ) , these data indicate that , under different biological conditions , different proteins may be responsible for activating apoptosis and provide evidence for a differential regulation of the multidomain Bcl 2 protein encoding genes , bax and bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As shown by the immunoblot analysis , our data clearly demonstrated that sanguinarine treatment to HaCaT cells resulted in a dose dependent ( a ) increase in the level of Bax with a concomitant decrease in Bcl 2 levels and ( b ) increase in Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METH induced apoptotic cell death and mRNA expression of pro apoptotic proteins ( bax and DP 5 ) , but not anti apoptotic proteins ( bcl 2 and bcl XL ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , there was a downregulation of Bcl 2 , shifting the equilibrium Bcl 2 / Bax toward the apoptotic pathway . ^^^ The cadmium induced decrease in Bcl 2 was inhibited , whereas the Bax levels were maintained closer to the control values . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis was performed to detect the changes of apoptosis related genes bcl 2 and bax protein expression . ^^^ CONCLUSION : Tubeimoside 1 can induce the apoptosis of CNE 2Z cells , and the induction of apoptosis by tubeimoside 1 is closely associated with downregulation and phosphorylation of bcl 2 and bax activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The percent age of apoptotic cells and positive expressions of p 53 , bax and bcl 2 , fas , fasL proteins were determined by FCM . ^^^ NADH could not only eliminate the apoptosis induced by 10 ray irradiation , but also up regulate expression of bcl 2 protein and down regulate expression of p 53 , bax , fas and fasL proteins ( P < 0 . 05 ) . ^^^ CONCLUSION : NADH has marked anti radiation effect , its mechanism may be associated with up regulation of bcl 2 expression and down regulation of p 53 , bax fas and fasL expression , as well as decline of intracellular ROS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased rate of apoptosis of epithelial cells in SS may result from either the imbalance between the down regulated apoptosis inhibitor Bcl 2 and the up regulated apoptosis inducer Bax , or the autocrine and / or paracrine Fas / FasL interaction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis index increased by 36 % and the expression of Caspase 3 and Bax and Bcl 2 increased markedly , but the expression of NF kappa B was decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twenty four , forty eight , and seventy two hours later , nested RT PCR was used to detect t the expression of PPAR gamma mRNA , Western blotting technique was used to detect the expression of PPAR gamma protein , MTT staining was used to observe the proliferation of cells induced by PPAR gamma agonists , TUNEL method was used to observe the apoptosis of cells affected by the ligands of PPAR gamma , the expressions of bax , and bcl 2 mRN and proteins were examined by in situ hybridization and immunohistochemistry , and the expression of caspase 3 was detected by immunohistochemistry . ^^^ Caspase 3 and bax / bcl 2 play a role in this process , PPAR gamma is so important in the pathogenesis and / or progression of lung cancer that it may be a novel therapeutical target against lung cancer . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas the inhibitors of apoptosis , bcl 2 and Rb , were upregulated over 2 . 5 fold in infected ( 48 h ) alveolar epithelial cells , the proapoptotic genes , bad and bax , were downregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a member of the Bcl 2 family , translocates to mitochondria during apoptosis , where it forms oligomers which are thought to release apoptogenic factors such as cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hepatitis C virus NS5A as a potential viral Bcl 2 homologue interacts with Bax and inhibits apoptosis in hepatocellular carcinoma . ^^^ These results suggest that HCV NS5A as a Bcl 2 homologue interacts with Bax to protect p 53 negative HCC cells from NaPB induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the p 53 , Bax and Bcl 2 mRNA was measured by RT PCR amplification . ^^^ These observations were supported by the observed increase in the expression of the proapoptotic genes , p 53 and Bax , together with a decrease in the expression of the antiapoptotic gene , Bcl 2 , and consequently an increase in the Bax : Bcl 2 ratio in tumors from lutein fed mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the expression of bcl 2 , c myc and bax genes involved in apoptosis as well as p 65 , which function in this process is unknown , were determined . ^^^ Semi quantitative RT PCR showed a decrease in bcl 2 and an increase in bax , c myc and p 65 expression in HL 60 cells treated with carboplatin in combination with amifostine as compared to the cells treated only with carboplatin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis with LightCycler quantitative RT PCR demonstrated the decrease of bcl 2 and increase of bax mRNA levels . ^^^ These results suggest that MEHP can induce apoptosis in U 937 cells through modulation of the balance of bcl 2 / bax in part by PPARgamma activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inasmuch as Bcl 2 suppresses while Bax accelerates apoptosis , treatment of cells with morphine reduced the expression of Bax and enhanced the expression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we also measured the expression of Bcl 2 and Bax by quantitative RT PCR . ^^^ Results of this study suggest that the ion pair influenced ERK 1 and TNFR 1 signaling pathways and affected thiol metabolism , whereas Bcl 2 and Bax were expressed at late stages of the death process . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , overexpression of Bcl 2 also inhibited the activation associated conformational change of the proapoptotic protein Bax , and higher doses of Pc 4 and light were required to activate Bax in cells expressing high levels of Bcl 2 . ^^^ PDT regimens that photodamage Bcl 2 lead to activation of Bax , induction of apoptosis and elimination of the otherwise resistant tumor cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related proteins ( bax , bcl 2 , and p 53 ) and apoptosis were evaluated in these segments by immunocytochemical stain and terminal deoxynucleotidyl transferase mediated DNA nick end labeling assay . ^^^ There were significant differences in numbers of segments with overexpression of bcl 2 and bax in the study and control groups . ^^^ The myocardial contractile reserve was inversely associated with an overexpression of bcl 2 ( P < . 01 ) rather than bax . ^^^ In conclusion , the expression of bax and bcl 2 proteins in patients with DCM is enhanced and independent of p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because of structural homology with the transmembrane domain of Bcl 2 , the proapoptotic protein Bax has been proposed to be anchored to the outer membrane of mitochondria through its carboxy terminal end ( CT ) . ^^^ We took advantage of the absence of Bcl 2 family members in yeast to further investigate the role of Bax CT in its mitochondrial association and function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax protein in endometriosis ] . ^^^ OBJECTIVE : To study the pathophysiological effect of the expression of the apoptosis regulating protein Bcl 2 and Bax in endometrosis . ^^^ Bcl 2 and Bax expression were examined by immunohistochemical ABC staining with specific monoclonal antibodies . ^^^ The expression of Bcl 2 or Bax in the eutopic endometrium was the same as the normal endometrium and their immunoreactivity was found predominately in the glandular epithelial cells , showing obvious cyclic changes . 2 . ^^^ The Bcl 2 and Bax expression in adenomyosis was not the cyclic change in ectopic glandular cells . 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of ischemic preconditioning on myocardial bcl 2 , bax , p 53 gene expression during ischemia / reperfusion period in rabbits ] . ^^^ OBJECTIVE : To investigate the effects of ischemic preconditioning on myocardial bcl 2 , bax , p 53 gene expression during ischemia / reperfusion period in rabbits . ^^^ Apoptosis index ( AI ) and protein expression of Bcl 2 , Bax , and p 53 in the border zone of myocardium of ischemic area at risk were obtained with a flow cytometry . ^^^ Bcl 2 expressions was higher in Group IP than in Group IR , while bax and p 53 expressions were lower in Group IP than in Group IR . ^^^ CONCLUSION : The rabbit myocardial apoptosis induced by ischemic preconditioning inhibited ischemia reperfusion in vivo partly related to the modulating of bcl 2 , bax and p 53 expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nuclear translocation of p 53 accompanied the transactivation of bax and downregulation of bcl 2 . ^^^ The pyruvate related redox manipulation inhibited the H2O2 induced p 53 activation , restored the downregulated bcl 2 and the upregulated bax , and hence enhanced the bcl 2 / bax expression ratio . ^^^ Furthermore , it is suggested that stabilizing the expression of bcl 2 and bax genes by metabolic antioxidants may be an effective strategy for endothelial protection against oxidative stress . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As ( 2 ) O ( 3 ) induced apoptosis was associated with reduced deltapsi ( m ) , enhanced generation of intracellular ROS , decreased levels of intracellular GSH , release of cytochrome c and AIF from the mitochondria , activation of caspases , down regulation of Bcl 2 and Bcl 10 ( L ) , and up regulation of Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis in MDA MB 231 cells was independent of the P53 / p21 pathway mechanism and was accompanied by an increased ratio of Bax to Bcl 2 and c myc levels and activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These effects are involved in the inhibition of DNA fragmentation and up regulation of anti apoptotic protein Bcl 2 and down regulation of pro apoptotic protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIMS : To investigate whether specific patterns in the expression of apoptosis related proteins correlate with carcinoma type and / or prognosis METHODS : The expression of Fas , Bcl 2 , Bax , Bcl xl , cyclooxygenase 2 ( COX 2 ) , and inducible nitric oxide synthase ( iNOS ) was studied immunohistochemically and the extent of apoptosis and proliferation was investigated in 11 cases of intestinal type and in eight cases of diffuse type carcinoma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We characterized immunohistochemically the expression of cell cycle regulators p 63 , CD 29 , PCNA , p 53 , pro and antiapoptotic proteins bcl 2 , bax , caspase 3 and DNA breaks , as well as keratin 10 , 16 and 17 . ^^^ The intensity of bcl 2 staining was higher at the edge of diabetic ulcers compared with venous ulcers , whereas the intensity of bax staining was similar . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl 2 mRNA was decreased , p 21 ( WAF1 / CIP1 ) and bax mRNA was increased ( P < 0 . 05 ) , the expression of PCNA , Bcl 2 , and cyclin B 1 protein were decreased , Bax and p 21 ( WAF1 / CIP1 ) protein were increased in SKOV ( 3 ) exposed to 20 micromol / L Genistein for 48 hours ( P < 0 . 01 ) . ^^^ The onset of apoptosis in ovarian carcinoma cell is related to the up regulation of bax and down regulation of bcl 2 in mRNA and protein level induced by Genistein . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The activity of p 53 was assessed by the expression of selected p 53 regulated targets , i . e . p 21 ( WAF1 / CIP1 ) gene , mdm 2 , bcl 2 and bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis of the expression of various Bcl 2 family members in betulinic acid treated cells showed , surprisingly , a suppression of the expression of the proapoptotic protein Bax but no changes in Mcl 1 or Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the frequency of apoptosis in oral leukoplakia and its relationship with pathological parameters , including Ki 67 expression , and with the expression of p 53 , Bcl 2 and Bax proteins . ^^^ Based on counts of 10 fields at a magnification of 10 400 , mitotic , apoptotic and Ki 67 indices were calculated , and p 53 , Bcl 2 and Bax expressions were recorded . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax protein levels were markedly elevated in several tissues at different ages and Bcl 2 / Bax ratios were lower in many dwarf tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Enforced expression of PIM 2 ( 34 kDa ) kinase does not appear to regulate expression of BCL 2 , BCL xL , BIM , or BAX proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we have evaluated the upregulation of several genes associated with delayed cell death ( c fos , bax , and bcl 2 ) during the initial 24 h of transient middle cerebral artery occlusion ( MCAo ) in the rat and the effects of postinjury treatment with the NR2B subunit specific NMDA receptor antagonist CGX 1007 ( Conantokin G , Con G ) . ^^^ No significant differences were measured in either bcl 2 or bax mRNA expression between treatment groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast to Bax , the pro apoptotic BH 3 only Bcl 2 proteins Bim and Harakiri / DP5 were down regulated by kainic acid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor analysis showed that : a ) by 37 days post NMBA , control mice showed approximately 7 tumors and by 84 days approximately 10 tumors / forestomach ; b ) mice receiving FHIT virus at 2 or 42 days post NMBA showed significantly reduced tumor burdens ; c ) Fhit was still expressed at 82 days postinfection ; d ) control viral infection had no effect on tumor development ; and e ) reduced Bcl 2 , increased Bax expression , and increased TUNEL positive apoptotic nuclei characterized the restored epithelia of FHIT transduced forestomachs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , there was no change detected in Fas , Fas associated death domain , Bcl 2 , Bcl xl , Bax , p 53 , or XIAP ( 10 linked inhibitor of apoptosis protein ) expression up to 12 h after poly ( 1 : C ) transfection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , BBSKE decreased the protein level of Bcl 2 but increased the level of Bax , and induced apoptosis in PC 3 and DU 145 human prostate cancer cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chronic hypoxia protects against gamma irradiation induced apoptosis by inducing bcl 2 up regulation and inhibiting mitochondrial translocation and conformational change of bax protein . ^^^ Expression of Bax , Bcl 2 and P 53 was assessed by immunofluorescence and Western blotting . ^^^ Under chronic hypoxia , Bcl 2 increased considerably in cells compared to control , while Bax and P 53 did not change . ^^^ In conclusion , chronic hypoxia : 1 ) seems to slow down cell growth of KB 3 1 cells without inducing apoptosis , 2 ) induces Bcl 2 overexpression and prevents radiation induced apoptosis by inhibiting ROS production and altering Bax subcellular redistribution and conformational changes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To test this hypothesis , we treated endometrial cancer cells ( Ishikawa cell line ) with resveratrol ( 1 , 10 , 50 and 100 micro M ) for 1 , 3 , 5 and 7 days , and analyzed for growth signal genes ( EGF and VEGF ) , cell cycle regulatory genes ( p 53 and p 21 ) , and apoptosis related genes ( bcl 2 and bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with the induction of apoptosis , 15 deoxy PGJ 2 increased the expression of proapoptotic proteins caspase 3 , caspase 9 , and Bax but down regulated antiapoptotic protein Bcl 2 . 15 Deoxy PGJ 2 also activated extracellular signal regulated kinase ( ERK ) 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of Hsp 10 and Hsp 60 increased the abundance of the anti apoptotic Bcl xl and Bcl 2 , and reduced the protein content of the pro apoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The anti apoptotic Bcl 2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of carvedilol on cardiomyocyte apoptosis and expression of bcl 2 , bax genes following ischemia ( 0 . 5 h ) and reperfusion ( 48 h ) in vivo and the possible biological mechanism of carvedilol inhibiting cardiomyocyte apoptosis were studied . ^^^ Immunohistochemistry and in situ hybridization histochemistry ( ISHH ) were used to detect the expression of bcl 2 and bax genes . ^^^ Bcl 2 / bax ratio was 1 . 07 + / 0 . 14 ( I / R group ) , 1 . 28 + / 0 . 16 ( sham operated group ) , 2 . 5 + / 0 . 26 ( carvedilol treated group ) respectively with the difference being very significant between carvedilol treated group and I / R group ( P < 0 . 05 ) . ^^^ It was indicated that carvedilol could inhibit cardiomyocyte apoptosis following ischemia and reperfusion , which was related to the increased bcl 2 / bax ratio due to inhibition of bax gene expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of sodium butyrate on transcription of Bax and Bcl 2 was analyzed by RT PCR . ^^^ RT PCR showed sodium butyrate had little effects on transcription of Bax and Bcl 2 in HeLa cells . ^^^ CONCLUSION : Sodium butyrate can induce apoptosis in HeLa cells without changing the expression of Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TC resulted in the degradation of Bcl 2 , the translocation of Bax from the cytosol to mitochondria , and the release of cytochrome c from mitochondria . ^^^ Stable overexpression of human Bcl 2 could reduce the apoptosis of TC treated cells by blocking the translocation of Bax and the release of cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of a major apoptosis resistant gene , bcl 2 , was markedly suppressed at a period of the provirus expansion and bax was also down regulated . p 53 was consistently expressed at high levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In LNCaP cells , p 53 , p 21 , Bax , and hypophosphorylated retinoblastoma tumor suppressor protein ( pRb ) levels increased , whereas Bcl 2 , pRb protein , and E2F transcription factor 1 ( E2F1 ) levels decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because Bcl 2 family members are intimately involved in cell death processes , the present study used dopaminergic cultures from control , Bcl 2 overexpressing , or Bax deficient genetically modified animals to determine the in situ effects of parkinsonism inducing toxins . ^^^ MPP ( + ) mediated cell death was attenuated by Bcl 2 but did not require Bax . ^^^ Accordingly , mutations or deletions within Bax heterodimerization domains , BH 1 , BH 2 , or BH 3 had no effect on Bcl 2 ' s ability to prevent cell death , whereas the cell death suppressing BH 4 domain did . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altogether , these data indicate that 4 HPR induces MMP through an ROS mediated pathway that involves the obligatory contribution of the proapopotic Bcl 2 family members Bax and / or Bak . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After antigen driven expansion , the majority of T cells involved in an immune response die rapidly by apoptosis dependent on the Bcl 2 related proteins , Bim and Bax or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , in contrast to the anti apoptotic Bcl 2 proteins , BHRF 1 does not bind tightly to peptides derived from the pro apoptotic proteins Bak , Bax , Bik , and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Analysis of the expression of the members of the Bcl 2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl 2 , Bcl XL , Bcl XS , or Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULT ( S ) : In stroma , protein expression of estrogen receptor alpha , Bcl 2 , and Bax was higher in PCOSE than in NE ; epithelial cells had a greater expression of androgen receptor in the nucleus and a lower expression in the cytoplasm of PCOSE . ^^^ The bax mRNA expression was higher in PCOSE , and bcl 2 mRNA expression was similar between groups . ^^^ A higher bcl 2 / bax relative ratio in PCOSE was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A decrease in the anti apoptotic protein , Mcl 1 , was detected in HT and NE treated HL 60 cells , whereas other Bcl 2 family proteins including Bax , Bcl 2 , Bcl XL , and Bag remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative Western blots were performed to detect changes in bax , bcl 2 and caspase 3 . ^^^ Quantitative Western blots showed a shift of the bax : bcl 2 ratio toward proapoptotic bax in ADA treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The combination treatment of docetaxel plus PD 98059 also increased cellular apoptosis , which was in part due to the inactivation of Bcl 2 by increasing phosphorylated Bcl 2 by more than 6 fold and Bax expression by 3 fold over each agent alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased expression of Bcl 2 and Bax , but not of Bcl 10 , occurs in the penumbra at the time when Bax translocates from the cytosol to the mitochondria , cytochrome c is released to the cytoplasm and active caspase 3 is expressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We measured the expression of p 53 , p 21 , bcl 2 , bax and E2F 1 . ^^^ UV radiation induced high levels of bax at 2 and 24 h after irradiation with a concomitant decrease in bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , when the B CLL patients were analysed according to P2X7 genotype ( 1513 A / A versus 1513 A / C ) , there was no significant difference in age at diagnosis , stage at diagnosis , lymphocyte doubling time , time to first treatment , progression free survival and overall survival , and neither was there any evidence of bias in terms of VH gene mutational status , CD 38 expression , in vitro sensitivity to fludarabine or expression of Bcl 2 , Bax or Mcl 1 between the two groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To explain this intriguing differential sensitivity between unstimulated CD34+ cells versus those stimulated by Epo + KL , we examined the expression of apoptosis regulating genes ( FLIP , BCL 2 , BCL XL , BAD and BAX ) in these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantification of Bax and Bcl 2 in polymorphonuclear leukocytes from haemodialysis patients : relation to hydrogen peroxide . ^^^ BACKGROUND : Bax and Bcl 2 are two apoptosis related molecules that play an important role in determining cell fate following oxidative injury . ^^^ In the present study , we explored the relation of hydrogen peroxide ( H2O2 ) generation by polymorphonuclear cells ( PMNs ) to the cytosolic expression of Bax and Bcl 2 proteins and apoptosis in haemodialysis ( HD ) patients . ^^^ Bax and Bcl 2 expression was detected by flow cytometry using FITC conjugated antibodies . ^^^ To examine the effect of H2O2 on Bcl 2 and Bax expression , PMNs from control subjects were briefly exposed to H2O2 ( 0 . 1 100 microM ) for 10 min and then washed and cultured for 6 h , with or without catalase , a H2O2 detoxifying molecule . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression and subcellular distribution of Bcl 2 and BAX proteins in serum starved human keratinocytes and mouth carcinoma epidermoid cultures . ^^^ Using specific antibodies a study of the expression and subcellular distribution of Bcl 2 , BAX , caspase 3 and cytochrome c in normal human keratinocytes and mouth carcinoma slowly ( HN ) and rapidly growing ( KB ) cells has been carried out . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis and immuno histochemistry were used to verify inducible Bcl 2 and to analyze Bcl 2 and a proapoptotic member of the Bcl 2 family , Bax , subcellular distribution . ^^^ In the absence of bcl 2 overexpression and after treatment with PAF , Bax translocated to mitochondria , and mitochondrial membrane potential collapsed within 1 h , followed by caspase 3 activation , which peaked at 6 h with an ensuing DNA fragmentation maximizing at 18 h . ^^^ After IPTG induction of bcl 2 expression , PAF failed to induce DNA fragmentation , caspase 3 activation , Bax translocation , or a collapse of mitochondrial membrane potential . ^^^ These data are the first to show that PAF can activate apoptotic machinery in enterocytes via a mechanism involving Bax translocation and collapse of mitochondrial membrane potential and that both of these events are under control by bcl 2 expression levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To gain insight into the molecular mechanisms underlying chemotherapeutic drug resistance , we examined the role of the Bcl 2 family members Bcl 2 and Bax in cell death in the melanoma cell line G 361 following stimulation with cisplatin ( CDDP ) or dacarbazine ( DTIC ) . ^^^ CDDP induced apoptosis and secondary necrosis were accompanied by activation of caspase 3 and modulation of Bcl 2 family members Bcl 2 and Bax . ^^^ On Western blotting Bax was seen to be upregulated with concomitant downregulation of Bcl 2 . ^^^ Flow cytometry , which enables measurement of protein at the single cell level , revealed that Bcl 2+ / Bax cells were decreased , with a slight concomitant rise in Bcl 2 / Bax+ cells on stimulation with CDDP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These events were associated with Bcl 2 cleavage , Bax , Bak , and Bad accumulation , mitochondrial translocation of Bax , abrogation of Mcl 1 , Bcl xL , and XIAP upregulation , and a marked induction of JNK and p 53 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SFN induced apoptosis was associated with up regulation of Bax , down regulation of Bcl 2 and activation of caspases 3 , 9 and 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical staining was used to examine leukocyte differentiation antigens ( LCA , L 26 , UCHL 1 , PS 1 ) , viral gene products ( LMP 1 , EBNA 2 , BZLF 1 ) and cellular oncoproteins ( p 53 , C myc , Bcl 2 and Bax ) . ^^^ Positive rates of p 53 , C myc , Bcl 2 and Bax expression were 83 . 33 % , 100 % , 95 . 83 % , 91 . 67 % , respectively , in 24 cases of the EBV induced lymphomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cycloheximide and actinomycin D delay death and affect bcl 2 , bax , and Ice gene expression in astrocytes under in vitro ischemia . ^^^ Expression of bcl 2 ( alpha and beta ) , bax , and Ice in astrocytes under similar ischemic conditions , as measured by quantitative reverse transcription polymerase chain reaction , indicated that ischemia down regulated bcl 2 ( alpha and beta ) and bax . ^^^ CHX reduced the expression of bcl 2 ( alpha and beta ) but increased bax and Ice expression . ^^^ It is hypothesized that the balance of proapoptotic ( Bad , Bax ) and antiapoptotic ( Bcl 2 , Bcl Xl ) proteins determines apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of Bcl 2 , Bcl 10 and bax protein expression in psoriasis . ^^^ METHODS : Twenty six lesional biopsy samples of 26 patients with psoriasis and five control specimens from normal skin were studied by immunohistochemical method for the differential expression of pro apoptotic bax and antiapoptotic bcl 2 and bcl 10 proteins . ^^^ RESULTS : Compared with the normal epidermis , bcl 2 expression was significantly reduced , whereas bax and bcl 10 were significantly overexpressed in the psoriatic epidermis . ^^^ The localization of bcl 2 / bax / bcl 10 proteins in the psoriatic epidermis did not show a significant deviation from that in the normal epidermis . ^^^ CONCLUSION : These findings indicate a discordant expression of bcl 2 and bax / bcl 10 in psoriatic epidermis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family member Bax is an apoptosis promoting protein that normally resides in an inactive state within the cytoplasm of healthy cells . ^^^ Protein protein interactions between Bax and other Bcl 2 family members are strongly implicated in Bax activation , but a compelling case has recently been made for the involvement of lipids in this process as well . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some key molecules involving in proliferation ( cyclin E and cyclin dependent kinase 4 ) and apoptosis ( bcl 2 and bax ) were also determined , so as to interpret the mechanisms of the alteration of tissue repairing cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No alterations in Bax and Bcl 2 expression were found during in vivo chemotherapy , and CD 95 expression and sensitivity remained low , indicating the induction of apoptosis independent of the CD 95 system or regulation of protein levels of Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase was associated with no change in GLUT 1 ( blood brain barrier / glial isoform ) , monocarboxylate transporter isoforms 1 and 2 , synapsin 1 ( neuronal marker ) , or Bax ( proapoptotic protein ) but with a modest increase in Bcl 2 ( antiapoptotic mitochondrial protein ) protein concentrations . ^^^ At 24 h of reoxygenation , the increase in GLUT 3 disappeared but was associated with a decline in Bcl 2 protein concentrations and the Bcl 2 : Bax ratio , an increase in caspase 3 enzyme activity ( apoptotic effector enzyme ) , and extensive DNA fragmentation , which persisted later in time ( 48 h ) only in the hippocampus . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SNAP ( 0 . 5 mM ) lowered the expression of Fas and p 53 in luteinized granulosa cells , but Bcl 2 expression was increased , and Fas ligand or Bax remained unchanged . ^^^ Using L NAME ( 0 . 5 and 5 . 0 mM ) , the expression of p 53 and Bax was increased , but Bcl 2 was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nimesulide inhibits tumor growth in mice implanted hepatoma : overexpression of Bax over Bcl 2 . ^^^ Nimesulide also decreased cyclooxygenase 2 ( COX 2 ) , prostaglandin E 2 ( PGE 2 ) and Bcl 2 expression , while increased the level of Bax protein . ^^^ CONCLUSION : Nimesulide suppresses tumor growth and induces apoptosis by inhibiting COX 2 and PGE 2 expression , which may be related to overexpression of Bax over Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : TUNEL staining , immunohistochemical staining for Ki 67 , p 53 , Bcl 2 , Bcl 10 ( L ) and Bax were performed on paraffin embedded specimens obtained from 11 H . pylori negative controls , 20 H . pylori positive chronic gastritis , 10 chronic gastritis with intestinal metaplasia ( IM ) , 11 adenoma , and 7 adenocarcinoma patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Addition of fatty acids to liposomes in the presence of cations greatly enhanced the permeabilizing activity of Bax , a pro apoptotic Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These Grim GH 3 activities do not require Bax or Bak function , revealing GH 3 activity as the first proapoptotic stimulus able to trigger the mitochondrial death pathway in mammalian cells in the absence of multidomain proapoptotic Bcl 2 proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This review focuses on the interactions of two of the major pro apoptotic Bcl 2 family members , Bax and Bid , with mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic protein BCL 2 prevents this mitochondrial loop , while the `` multidomain ' ' proapoptotic proteins BAX and BAK are crucial to initiate it . ^^^ BCL 2 , BAX and BAK localize also to the endoplasmic reticulum ( ER ) , the main intracellular Ca ( 2+ ) store . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Specifically rasagiline activates Bcl 2 , Bcl xl , protein kinase C ( PKC ) and reduces Bax in a variety of cells including PC 12 and neuroblastoma human dopamine derived SH SY5Y cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in rhesus monkey testis during germ cell apoptosis induced by testosterone undecanoate . ^^^ To study the molecular mechanism of apoptosis induced by a high dose of testosterone undecanoate ( TU ) , the authors examined the temporal changes in proapoptotic Bax and antiapoptotic Bcl 2 in TU treated monkey testes . ^^^ The expression of Bcl 2 and Bax was assessed by immunohistochemical method and Western blot . ^^^ As compared with that of normal testes , the levels of Bcl 2 protein increased significantly from 7 to day 14 while that of Bax protein was almost unchanged in the testes from day 7 up to day 60 after TU treatment . ^^^ Therefore , it is suggested that Bax may not play a role in initiating germ cell apoptosis induced by TU injection and that the evaluation in Bcl 2 expression may represent a survival mechanism for the remaining germ cell . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In other studies , silibinin caused a moderate increase in phospho Bcl 2 , without any noticeable changes in total Bcl 2 levels , and down regulated bax levels moderately . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related molecules , bcl 2 , bax , caspase 3 , death receptor , Fas , and death substrate , poly ( ADP ribose ) polymerase ( PARP ) were studied . ^^^ Pro apoptotic bax and caspase 3 were downregulated , whereas bcl 2 was upregulated in the glycine treated animals ( P < . 02 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A significant decrease in Bcl 2 mRNA and a significant increase in Bax and p 53 mRNA further characterized the IUGR kidney . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Results indicate that Bcl 2 and caspase 2 expression is enhanced , while bax , GADD 45 and mdm 2 expression is reduced in irradiated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A 1 base pair deletion at the poly ( G ) 8 tract and no expression of Bax and Bcl 2 proteins were identified in one microsatellite instability high endometrial endometrioid carcinoma . ^^^ Immunohistochemical staining for Bax and Bcl 2 proteins was negative on the tumor specimen that had this 1 base pair deletion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The animals were then killed , and brains underwent paraffin embedding for hematoxylin eosin staining , in situ terminal deoxynucleotidyl transferase mediated deoxy UTP nick end labeling ( TUNEL ) staining and immunohistochemistry ( bax , bcl 2 ) . ^^^ About 95 % neurons in hippocampal CA 1 area entered apoptosis and only 2 % 7 % pyramidal neurons stayed alive due to an inhibition of bcl 2 expression and an increase in bax expression . ^^^ Infusion of pergolide could induce the expression of bcl 2 and reduce the expression of bax . ^^^ All these results indicate that pergolide plays an important role in the protection of hippocampal neurons from apotosis through upregulating the expression of bcl 2 protein and reducing the expression of bax protein . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It induced apoptosis as shown by : ( 1 ) decreased Bcl 2 and Bcl 10 ( L ) proteins and increased Bax protein ; ( 2 ) activation of caspase 3 ; and ( 3 ) increased shedding of apoptotic cells in the medium . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of magnesium sulfate on neuron apoptosis and expression of caspase 3 , bax and bcl 2 after cerebral ischemia reperfusion injury . ^^^ OBJECTIVE : To investigate the effects of magnesium sulfate on neuron apoptosis and the expressions of caspase 3 , bax and bcl 2 after cerebral ischemia reperfusion injury . ^^^ The neuron apoptosis was detected by the terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP fluorescence nick end labeling ( TUNEL stain ) , and the protein expressions of caspase 3 , bax and bcl 2 were detected by immunohistochemisty stain . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : With in situ hybridization and immunohistochemistry staining methods , PKC , pro apoptotic , and anti apoptotic gene ( Bax , bcl 2 ) expressions were measured in nasal polyp tissues from 26 patients and inferior turbinate mucosa tissues ( ITMTs ) from 20 healthy persons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intriguingly , Western blot analyses demonstrated significant down regulation of Bcl 2 protein , but no alteration in Bax protein expression in the SN after thrombin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression profiles of various apoptosis regulating proteins ( including Bcl 2 , Bax , p 53 and caspase 3 ) were well correlated to the alterations of nuclear morphology depicted by 4 , 6 diamidino 2 phenylindole ( DAPI ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of Bax translocation through phosphorylation at Ser 70 of Bcl 2 by MAP kinase in NO induced neuronal apoptosis . ^^^ The molecular mechanism of Bcl 2 phosphorylation and its relationship to Bax is largely unknown . ^^^ Here we show that the phosphorylation of Bcl 2 is involved in the intracellular translocation of Bax from cytosol to mitochondria in NO induced neuronal apoptosis . ^^^ Furthermore , we investigated whether Bcl 2 phosphorylation affected Bax translocation , using mutant Bcl 2 expression vectors . ^^^ Cortical neuronal cells overexpressing the Bcl 2 mutant S70A ( which can not be phosphorylated ) prevented the translocation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax are involved in experimental cryptorchidism induced testicular germ cell apoptosis in rhesus monkey . ^^^ Using an experimental cryptorchidism model in rhesus monkey , we have investigated the relationship between apoptosis and the Bcl 2 family members , Bcl 2 and Bax . ^^^ The expressions of Bcl 2 and Bax in the testes during the heat stress induced testicular germ cell apoptosis were detected by immunohistochemistry and Western blot techniques . ^^^ The subcellular redistribution of Bax and the increase in Bcl 2 expression in the cryptorchid testis suggest an involvement of Bcl 2 family members in heat stress induced germ cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA expression of Bax and Bcl 2 was investigated by quantitative real time PCR . ^^^ The mRNA levels of both Bax and Bcl 2 were increased after 30 min . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Microvasc Res 66 : 91 101 , 2003 ) ; this was associated with increased expression of apoptosis related bcl 2 and decreased expression of bax gene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ATRA , 9 cis RA and 4 HPR initiated the highest level of apoptosis as determined by mitochondrial Bax translocation , while only ATRA and 9 cis RA strongly reduced Bcl 2 and survivin protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , we found that formation of TR3 / RXRalpha heterodimers in the nucleus and their subsequent translocation into the cytoplasm , in association with regulation of apoptosis related proteins Bcl 2 , Bcl xl and Bax , was critical for apoptosis induction by ATRA in breast cancer cells MCF 7 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In situ hybridization studies of treated cultures revealed that expression of the apoptotic suppressor , bcl 2 , remained consistently high throughout the culture period , whilst the apoptotic inducer , bax , was not expressed until day 23 . ^^^ Quantification of these data showed a gradual shift in the ratio of the expression level of bcl 2 and bax in favour of bax with time in culture , particularly from day 23 onwards . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of IFNgamma on caspase 3 , Bcl 2 and Bax expression , and apoptosis in rabbit placenta . ^^^ The purpose of this study was to determine whether apoptosis in placenta was affected by IFNgamma , which can induce abortion , and whether the effect of IFNgamma on apoptosis resulted from an intrinsic program of apoptosis , which was regulated by Bcl 2 and Bax . ^^^ Expression of Bcl 2 and Bax in placenta was evaluated by immunoblot analysis and immunohistochemistry study . ^^^ Both Bcl 2 and Bax expression was decreased 0 . 44 fold and 0 . 46 fold by 50 , 000 IU IFNgamma and 0 . 41 fold and 0 . 03 fold by 100 , 000 IU IFNgamma . ^^^ This resulted in change of a 0 . 07 fold increase in the Bax : Bcl 2 ratio in 50 , 000 IU IFNgamma treated groups and 0 . 41 fold increase in 100 , 000 IU IFNgamma treated groups as compared with those in control groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition to the CD 95 and TNFalpha systems , the expression of apoptotic molecules bcl 2 , b myc , c myc , bax , max , mad and IL1alpha was induced by FB 1 in TKO mice to a greater extent than in WT animals ; many of these factors also had a higher constitutive expression in TKO animals than WT mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic family proteins such as Bcl 2 and Bcl 10 ( L ) function , at least in part , by binding proapoptotic members such as Bax and Bak and thereby preventing release of apoptotic proteins , including cytochrome c , from the mitochondria . `` BH 3 only ' ' members of the family disrupt this interaction by binding , via their BH 3 domain , to a hydrophobic pocket on the surface of the antiapoptotic members . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The second protocol details subcellular fractionation of adipose tissue for the extraction of protein in the mitochondrial and cytosol fractions and the measurement of apoptotic protein ( Bcl 2 and Bax ) levels in each fraction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we found that NPCs deficient in Apaf 1 , or both the pro apoptotic multidomain Bcl 2 family members Bax and Bak , were resistant to cytosine arabinoside and gamma irradiation induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate the role of mitochondria after infection of cells with ncp BVDV , expression of Bcl 2 and Bax were analysed . ^^^ It was shown that while Bax expression was not affected , the anti apoptotic Bcl 2 protein was upregulated , presumably suppressing initiation of cell death and enabling persistent infection in vitro . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , evodiamine induced activation of caspase 3 resulted in the down regulation of anti apoptotic Bcl 2 expression and up regulation of proapoptotic Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , chemotherapy did not cause a compatible change in expression levels of proteins such as Bcl 2 , Bcl 10 ( L ) , Bax , cIAP 2 , XIAP and survivin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , roscovitine modified the expression of cytochrome c ( Cyt c ) , Bcl 2 and Bax , which are involved in the apoptotic intrinsic pathway induced by S / K deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the mechanisms responsible for survival and apoptosis / anoikis in normal human intestinal epithelial crypt cells , we analyzed the roles of various signaling pathways and cell adhesion on the expression of six Bcl 2 homologs ( Bcl 2 , Bcl XL , Mcl 1 , Bax , Bak , Bad ) in the well established HIEC 6 cell model . ^^^ For example , the inhibition of the PI 3 K / Akt 1 pathway down regulated Bcl XL , Mcl 1 , and Bad , while at the same time up regulating Bax , whereas the inhibition of Fak up regulated both Bax and Bak , down regulated Bad , and did not affect the other Bcl 2 homologs analyzed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax in extrahepatic biliary tract carcinoma and dysplasia . ^^^ AIM : To compare the difference of expression of Bcl 2 and Bax in extrahepatic biliary tract carcinoma and dysplasia , and to analyze the role of Bcl 2 and Bax proteins in the progression from dysplasia to carcinoma and to evaluate the correlation of Bcl 2 / Bax protein expression with the biological behaviors . ^^^ METHODS : Expressions of Bcl 2 and Bax were examined immunohistochemically in 27 cases of extrahepatic biliary tract carcinomas ( bile duct carcinoma : n=21 , carcinoma of ampulla of Vater : n=6 ) , and 10 cases of atypical dysplasia . ^^^ A semiquantitative scoring system was used to assess the Bcl 2 and Bax reactivity . ^^^ Bcl 2 and Bax activities were more intense in carcinoma than in dysplasia , with no significant difference in Bcl 2 expression ( P=0 . 110 ) , and significant difference in Bax expression ( P=0 . 038 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , beta sitosterol induced apoptosis in HT 116 cells was associated with a decreased expression of the anti apototic Bcl 2 protein and mRNA and a concomitant increase of the pro apototic Bax protein and mRNA , and with release of cytochrome c from the mitochondria into the cytosol . beta sitosterol treatment also inhibited the expression of cIAP 1 without significant changes in the level of cIAP 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the induction of apoptosis , expression of p 53 and p21WAF1 was upregulated , phosphorylation of IkappaB alpha was blocked , caspase substrates poly ( ADP ribose ) polymerase ( PARP ) and lamin B were cleaved , and Bcl 2 and Bax protein expression remained unchanged . ^^^ However , Bcl 2 and Bax are not associated with manumycin mediated apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This family of proteins now includes both anti apoptotic molecules such as Bcl 2 and Bcl 10 ( L ) , and pro apoptotic molecules such as Bax , Bak , Bid , and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was preceded by increases in tissue ( but not plasma ) angiotensin 2 concentration ; enhanced expression of phosphorylated mitogen activated protein ( MAP ) kinases p 38 , JNK , and ERK ; and augmented ratios of the proapoptotic protein Bax to the antiapoptotic protein Bcl 2 . ^^^ ACE inhibition ( enalapril ) prevented increases in tissue angiotensin 2 concentration , phosphorylated ERK expression , Bax / Bcl 2 ratio , and cellular apoptosis , but did not affect total cell death , leukocyte infiltration , JNK or p 38 activation , and reduced but did not eliminate tissue fibrosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further investigate the underlying mechanisms , caspase 3 activity assay and mRNA expression of Bax , Bcl 2 , and growth hormone secretagogue receptor ( GHS R ; the supposed hexarelin binding site ) were examined . ^^^ These results suggest that hexarelin abates cardiomyocytes from ANG 2 induced apoptosis possibly via inhibiting the increased caspase 3 activity and Bax expression induced by ANG 2 and by increasing the expression of Bcl 2 , which is depressed by ANG 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The distribution of Bcl 2 and Bax in the cytosol of aged rats dramatically decreased the Bcl 2 / Bax ratio , a probable indicator of neuronal vulnerability , which was restored upon treatment with PBN . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of hypocapnia ( PaCO 2 27 mmHg ) on CaM kinase 4 activity , Bax / Bcl 2 protein expression and DNA fragmentation in the cerebral cortex of newborn piglets . ^^^ Bcl 2 protein density was the same in both groups , resulting in an increased ratio of Bax / Bcl 2 in the HC group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our objective was to determine the influence of a specific COX 2 inhibitor , rofecoxib ( Vioxx ) , on serum and tumor levels of gastrin and its precursor , progastrin , as well as on tumor gene expression of COX 2 , peroxisome proliferator activated receptor gamma ( PPARgamma ) , and apoptosis related proteins ( Bax and Bcl 2 , caspase 3 , and survivin ) . ^^^ Tumor gene and protein expressions of COX 2 , PPARgamma , Bax and Bcl 2 , caspase 3 , and survivin were determined by RT PCR and western blot . ^^^ Treatment of GC patients with Vioxx resulted in a significant decrease in plasma and tumor contents of both progastrin and gastrin , and this was accompanied by the increment in tumor expression of COX 2 , PPARy , Bax , and caspase 3 with a concomitant reduction in Bcl 2 and survivin expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Thus , oxygen deprivation induced cell death in fibroblasts with deregulated expression of c Myc is independent of p 53 or HIF 1 status , but is dependent on the Bcl 2 family member Bax or Bak to initiate mitochondrial dependent cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The rate of apoptosis and expression of Bcl 2 and Bax in leukocytes of acute myeloblastic leukemia patients . ^^^ The rate of apoptosis as well as expression of Bcl 2 and Bax was evaluated before and after induction therapy in leukocytes of 70 patients with acute myeloblastic leukemia ( AML ) , retrospectively divided into group A ( with longer survival ) and group B ( with shorter survival ) . ^^^ In this group , the Bcl 2 / Bax ratio was similar to the control and remained unchanged after treatment . ^^^ In both groups of untreated patients , western blot analysis revealed the presence of prominent additional bands reacting with anti Bcl 2 or anti Bax antibody , which were undetectable in control leukocytes . ^^^ In conclusion , the lack of therapy induced enhancement in leukocyte apoptosis , an increased ratio of Bcl 2 / Bax as well as persistent presence of abnormal Bcl 2 and Bax protein bands after induction therapy in AML patients may be considered as factors associated with unfavorable clinical outcome . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In hypoxic renal cells , Bax and Bak , 2 pro apoptotic proteins of the Bcl 2 family , collaborate to permeabilize the mitochondrial outer membrane to intermembrane proteins such as Cyt c , although Bax , per se , appears to play the dominant role . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We examined mitochondrial signaling , focusing on Bcl 2 and Bax by real time polymerase chain reaction ( PCR ) , immunocytochemistry , and immunoprecipitation . ^^^ The expression of Bcl 2 mRNA decreased to 0 . 45 + / 0 . 15 fold at 24 hours , whereas that of Bax increased to 7 . 3 + / 1 . 2 fold 1 hour after reoxygenation , accompanied by translocation from the cytosol to mitochondria . ^^^ Both overexpression of Bcl 2 and inhibition of Bax protected GENs from hypoxic injury . ^^^ CONCLUSION : We conclude that changes of quantity and localization of Bcl 2 and Bax contribute to hypoxia mediated apoptosis of GENs in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA for bax and bcl 2 was determined by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The bax mRNA was enhanced , while bcl 2 mRNA was reduced , after exposure to ioversol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic index was increased 4 7 fold , Bcl 2 protein expression decreased , Bax increased , and caspase 9 , caspase 6 , and caspase 7 were activated but not caspase 3 and caspase 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression level of some of these proteins , such as BCL 2 , BAX , and caspase 3 , has been shown to be predictive of ultimate outcome in hematopoietic tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Upon loss of the MCL 1 BAK complex , BAK complexed with either BAX in proapoptotic E1B mutant adenovirus infected cells , or with the adenovirus BCL 2 homolog E1B 19K in cells infected with the wild type virus in which apoptosis is inhibited . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ether lipid resistant S49ar cells were cross resistant to extracellular stress factors ( cold shock , heat shock , H2O2 , dimethylsulfoxide ) and to radiation induced apoptosis but not to physiological apoptotic signals ( dexamethasone , growth factor deprivation , thapsigargin , C 2 ceramide ) and expressed similar levels of the apoptosis regulating proteins Bcl 2 , Bcl 10 , Bax , Bad and Bak as did the parent S49wt cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Central to the regulation of the mitochondrial checkpoint is a complex three way interplay between members of the BCL 2 family , which are comprised of an antiapoptotic subgroup including BCL 2 itself , and the proapoptotic BAX , BAK and BH 3 domain only subgroups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Some studies have indicated that the death of Purkinje cells occurs by apoptosis , and the secondary loss of granule neurons has been shown to require the pro apoptotic Bcl 2 family member Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We searched evidence for apoptotic cell death in temporal lobes resected from drug resistant epilepsy patients with MTS by using the terminal deoxynucleotidyl transferase ( TdT ) and digoxigenin 11 dUTP ( TUNEL ) method and immunohistochemistry for Bcl 2 , Bax , and caspase cleaved actin fragment , fractin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax proteins in rat liver fibrosis model and the role of IFN gamma ] . ^^^ OBJECTIVE : To study the expression of Bcl 2 and Bax in rat liver fibrosis model induced by CCl 4 and the role of IFN gamma . ^^^ Bcl 2 and Bax proteins expression were detected by immunohistochemistry . ^^^ Compared with that in fibrosis model rats , the expression of Bax was significantly lower in rats treated with IFN gamma ( 9 . 80 % + / 3 . 75 % vs 5 . 85 % + / 2 . 35 % , t=2 . 98 , P < 0 . 01 ) , but the expression of Bcl 2 was not significantly different ( t=1 . 49 , P > 0 . 05 ) , however it was significantly lower in fibrous septae in IFN gamma treated group than in model group ( 6 . 58 % + / 4 . 13 % vs 9 . 46 % + / 4 . 29 % , t=2 . 80 , P < 0 . 05 ) . ^^^ CONCLUSION : The expression of Bcl 2 and Bax increases in liver fibrosis model rats , and IFN gamma can promote myofibroblasts apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 in SMMC 7721 cells treated by 3 mmol / L or 10 mmol / L aspirin decreased markedly , but no effect on Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hydrogen peroxide increases a 55 kDa tyrosinase concomitantly with induction of p 53 dependent p 21 waf1 expression and a greater Bax / Bcl 2 ratio in pigmented melanoma . ^^^ In the latter cells , this response also increases p 53 concomitant with joint induction of p 53 activated proteins like the cell cycle inhibitor p21WAF1 and pro apoptotic bax , with no comparable effect on expression of anti apoptotic bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA expression levels of HO 1 , VEGF ( 165 ) , Bcl 2 , Bax , and hypoxia inducible factor 1alpha were quantified by real time reverse transcriptase PCR , and the HO 1 and VEGF proteins were analyzed by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When c Myc , Bcl 2 and Bax expression and caspase 3 activity were measured , using Ac DEVD AMC as a substrate , the changes in these apoptosis regulatory and effector proteins suggested that the elevation of c Myc , decrease in Bcl 2 : Bax protein ratio , and caspase 3 activation all play a key role in apoptosis . ^^^ In addition , c Myc expression decreased and Bcl 2 : Bax ratio increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We aimed to describe the expression of Bax , Bcl 2 , iNOs and Hsp 70 in the endothelium and myocytes of both ventricles and to see if there is any relationship with clinical donor organ failure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , expression of Mcl 1 , Bid , Bcl 2 , and Bax were investigated in septic neutrophils . ^^^ Mcl 1 , Bid , Bcl 2 , and Bax mRNA expression was detected by reverse transcriptase polymerase chain reaction and protein determined by Western blot analysis . ^^^ No Bcl 2 mRNA or protein was detected in neutrophils from patients or controls , and detectable Bax protein and mRNA levels remained unchanged in all samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in the p 53 expression and SAPK / JNK activation after 10 irradiation was also inhibited by a Trolox treatment , but the expression of BCL 2 and BAX , which would occur downstream from p 53 , was not changed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic action of prolactin is associated with up regulation of Bcl 2 and down regulation of Bax in HC 11 mouse mammary epithelial cells . ^^^ The effect of prolactin on apoptosis and the expression of bcl 2 and bax in HC 11 mouse mammary epithelial cells were investigated . ^^^ In conclusion , the results presented indicate that stimulation of bcl 2 expression with simultaneous suppression of bax may be key events in the mechanism of antiapoptotic action of prolactin in HC 11 mammary epithelial cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This includes well established events such as activation of BCL 2 via translocations in follicular lymphoma , as well as more recent observations implicating activation of Bcl 10 ( L ) expression and frameshift and missense mutations of BAX and BCL 2 in cancer . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Evaluation of apoptosis associated gene expression in NaBT pre treated cultures incubated with DEX or DEX+PRL indicated that the apoptosis resistance did not stem from altered bcl 2 or bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax , Bcl 2 , Waf 1 , and PCNA gene products in an immortalized human endothelial cell line undergoing p 53 mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Subsequently , increased Bax expression was observed in the cytoplasm of dying cells located in the infarct , whereas an increased Bcl 2 and hsp 72 staining was detectable in survival cells and reactive glia present at the periphery of the lesion . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax modify this pathway or directly regulated by p 53 . ^^^ Among four cell lines RT 4 with wt p 53 , low ratio of Bcl 2 to Bax and induction of p21WAF1 / CIP1 after ADM treatment , was the most sensitive to induction of apoptosis . ^^^ Thus , p 53 , p21WAF1 / CIP1 , Bcl 2 and Bax status might determine susceptibility of bladder cancer cells to ADM induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to understand a possible involvement of genes regulating apoptotic signal pathways , expression levels of bcl 2 , bax , dapk 1 , myc , bad , wt 1 , and mcl genes were analyzed during ATRA treatment in five APL patients with t ( 15 ; 17 ) using Real time PCR ( LightCycler ) . ^^^ Ratio of bcl 2 to bax may be more important for this regulation than the ratio of bcl 2 to bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Human prolactin and its antagonist , hPRL G129R , regulate bax and bcl 2 gene expression in human breast cancer cells and transgenic mice . ^^^ To gain insight into the molecular mechanisms involved in human prolactin receptor antagonist ( hPRL G129R ) induced apoptosis , we used real time reverse transcription polymerase chain reaction to measure bax and bcl 2 gene expression in 11 human breast cancer cell lines following treatment with hPRL and hPRL G129R . ^^^ We also measured bax and bcl 2 gene expression in the mammary glands of transgenic mice expressing hPRL or hPRL G129R . ^^^ A time course study of hPRL and antagonist treatment in T 47D cells indicated changing bax / bcl 2 mRNA ratios beginning at 24 h . ^^^ We found that bax / bcl 2 mRNA ratios were significantly elevated in seven of the 11 hPRL G129R treated cell lines , as well as in the hPRL G129R transgenic mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of PC 12 cell cultures with Abeta ( 25 35 ) did not significantly alter total cellular expression levels of Bax , a proapoptotic Bcl 2 family member , or levels of Bcl XL , an antiapoptotic analogue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OA induced apoptosis involves disruption of F actin cytoskeleton , activation of caspase 3 , collapse of mitochondrial membrane potential , DNA fragmentation and decreased levels of monomeric Bcl 2 and Bax proteins . ^^^ All the agents tested were unable to obliterate changes in F actin levels , caspase 3 activation or DNA fragmentation , but all of them prevented OA induced decrease of mitochondrial potential and changes in Bax / Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Characteristics of Bcl 2 and Bax expressions in rat brain after experimental intracerebral hemorrhage and intervention of naoyian granule ] . ^^^ OBJECTIVE : To investigate the characteristics of Bcl 2 and Bax expressions in rat brain after experimental intracerebral hemorrhagic ( ICH ) , and to determine the protective mechanisms of the Traditional Chinese medicine complex , naoyian granule ( NYA ) . ^^^ Bcl 2 and Bax expressions in the rat brain after ICH were detected by immunohistochemistry method . ^^^ Both Bax and Bcl 2 expressed simultaneously in the marginal region of the cerebral hemorrhagic core . ^^^ CONCLUSION : The expressions of Bcl 2 and Bax after the cerebral hemorrhage are related to the degree of injury , the time , and the location of the brain tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Lithium increased the activities of MEK , ERK 1 / 2 , RSK 1 , and CREB , up regulated the expression of Bcl 2 , and down regulated the expression of Bax in rat brain hippocampus and frontal cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro death Bax increased , while anti death Bcl 2 and Bcl XL decreased , and apoptotic TUNEL positive cells were detected in the hippocampus of klotho mutant mice at the age of 7 weeks . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examination of apoptosis related proteins by immunoblot analysis revealed levels of BCL 2 , BCL 10 ( L ) , and Bax to be unaffected by celecoxib . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the basis that Bax and Bad were augmented in B 1a cells , and Bcl 2 and Bcl xL reduced , we conclude that the disappearance of B 1a cells , but not B 1b , in IL 10 / mice results from their enhanced susceptibility to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MIF , like G CSF , delayed cleavage of the proapoptotic members of the Bcl 2 family Bid and Bax in neutrophils , suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Modulation of apoptotic responses by over expression of anti apoptotic proteins ( NF kappaB , IAPs and Bcl 2 ) , or attenuation of pro apoptotic proteins ( PTEN and Bax ) may be responsible for the variations in sensitivity of these cell lines to hormone and chemotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In patients with severe dilated cardiomyopathy , we evaluated cardiomyocyte apoptosis in relation to the transmural distribution of Bax and Bcl 2 proteins ( 2 molecules inhibiting or promoting apoptosis , respectively ) and left ventricular wall stresses . ^^^ The expression of Bcl 2 and Bax proteins were determined with both Western analysis and immunohistochemistry . ^^^ The ratio of Bax to Bcl 2 proteins ( Bax / Bcl 2 ) was similar in the midmyocardium or subepicardium , but increased in the subendocardium , where it was directly related to systolic wall stress ( y = 0 . 009x 0 . 629 ; r 2 = 0 . 85 , P < . 001 ) . ^^^ CONCLUSIONS : In patients with dilated cardiomyopathy , in whom cardiomyocyte apoptosis is an important cause of cell loss , apoptosis is more extensively localized in the subendocardium and strictly related to ventricular wall stresses and the Bax / Bcl 2 ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis by p 53 , bax , bcl 2 , and p 21 expressed in colorectal cancer . ^^^ METHODS : In 30 colorectal cancer patients , we examined the mRNA expression of p 53 , bax , bcl 2 , and p 21 ( WAF 1 ) , and we also investigated the development of tumor cell apoptosis , using a terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP biotin nick end labeling ( TUNEL ) method . ^^^ CONCLUSIONS : The present study findings suggest that tumor cell apoptosis is ( 1 ) strongly inhibited by p 21 , ( 2 ) induced by bax , and ( 3 ) influenced by bcl 2 , which , presumably , inhibits tumor cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of this study was to evaluate odontogenic myxomas for the expression of cell cycle protein Ki 67 , apoptosis regulating proteins Bcl 2 , Bcl XL , Bak , and Bax , and matrix metalloproteinases MMP 2 , MMP 3 , and MMP 9 . ^^^ Twenty six paraffin embedded tissue sections were used in a standard immunohistochemistry protocol and incubated with one of the following antibodies : Bcl 2 , Bcl XL , Bak , Bax , or Ki 67 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , while anti apoptotic markers , Bcl 2 and phosphorylated Bad , were down regulated , pro apoptotic markers , active caspase 3 and Bax , were up regulated , resulting in the appearance of apoptotic cells in the penile tIssues of OLETF rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As a complement to this , the apoptotic regulators Bax , Bcl 2 , caspase 3 and phosphorylated c jun were studied . ^^^ The relative mRNA levels for both Bax and Bcl 2 were higher at 4 days after transection when compared to normal . ^^^ Immunoreactivity for Bax , Bcl 2 as well as for caspase 3 and phosphorylated c jun , indicative of cell death , localized to True Blue identified retinal ganglion cells 4 days after injury . ^^^ In addition , optic nerve transection caused an increase in Bim , Bax , and Bcl 2 mRNA levels in optic nerve and superior colliculus . ^^^ Our results suggest that Bim is involved in injury induced retinal ganglion cell death and indicate that the increase in Bim and Bax expression promote cell death of axotomized retinal ganglion cells whereas the elevation in Bcl 2 in retina may contribute to the control of the extent of apoptosis after the optic nerve transection . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Because Bcl 2 family proteins show structural similarity to diphtheria toxin ( DT ) , we constructed a unique retroviral IT using Bax , a proapoptotic member of the Bcl 2 family , in place of DT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intermittent hypoxia attenuates ischemia / reperfusion induced apoptosis in cardiac myocytes via regulating Bcl 2 / Bax expression . ^^^ Expressions of apoptosis related proteins , Bax and Bcl 2 , in cytosolic and membrane fraction were detected by Western Blotting . ^^^ Intermittent hypoxia attenuated ischemia / reperfusion induced apoptosis via increasing the ratio of Bcl 2 / Bax , especially in membrane fraction . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the end of experiment , the animals were killed by decapitation , blood samples were obtained , thyroid tissues were collected and processed for quantitative evaluation of immunohistochemical PCNA ( marker of cell proliferation ) , Bax ( pro apoptotic marker ) and Bcl 2 ( anti apoptotic marker ) staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of the NO production led to decreased rate of apoptosis accompanied by downregulation of the proapoptotic molecule Bax and increased expression of the antiapoptotic molecule Bcl 2 and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor expression of Ki 67 , BCL 2 , BAX , P 53 or c erbB 2 proteins was evaluated immunohistochemically on paraffin embedded tissues and their prognostic impact analyzed . ^^^ BCL 2 , BAX and P 53 proteins were expressed in 52 , 54 and 71 % of the tumors , respectively , while HER 2 protein was overexpressed in 16 % . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The evaluations of bax and bcl 2 expression were utilized by immunohistochemistry . ^^^ Apoptotic cells expressed and contained significantly higher proportions of both bax and bcl 2 proteins ( P < 0 . 05 ) . ^^^ Apoptosis induced by TNF alpha can increase the expressions of bax and bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry assay ( SP method ) was applied to determine the localization and immunoreactive intensity of proliferating cell nuclear antigen ( PCNA ) , estrogen receptor ( ER ) , progesterone receptor ( PR ) , B cell leukemia lymphoma 2 ( bcl 2 ) , bcl 2 associated 10 protein ( bax ) and CD ( 44v6 ) . ^^^ CONCLUSIONS : The study indicates that high dose progesterone could inhibit the growth , promote apoptosis and inhibit metastasis of endometrial carcinoma , MIF could inhibit the growth and promote apoptosis , MIF + MPA could more strongly inhibit the growth , promote apoptosis and inhibit metastasis of endometrial carcinoma than MIF or MPA , and synergistic effect was observed on the expression of PCNA , ER , PR , bax and bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While the expression of p 53 was increased , the level of Bcl 2 and Bax was decreased . ^^^ Our experiments suggest that BAG 1 inhibits anti cancer drug induced apoptosis through apoptosis regulation pathways that may involve the mitochondrial Bcl 2 / Bax ratio , p 53 , and differential anti cancer drug mediated cytochrome c release . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Potential role of bcl 2 and bax mRNA and protein expression in chronic hepatitis type B and C : a clinicopathologic study . ^^^ Bcl 2 oncoprotein regulates programmed cell death by providing a survival advantage to rapidly proliferating cells , and bax protein promotes apoptosis by enchanting cell susceptibility to apoptotic stimuli . ^^^ In this study , we assessed the expression of bcl 2 and bax in liver biopsies from patients with chronic hepatitis ( CH ) Type B ( HBV ) and C ( HCV ) . ^^^ Pathologic examination included the following : ( 1 ) immunohistochemical stains in paraffin sections for bcl 2 and bax protein expression , ( 2 ) Western blot analysis ( bcl 2 and bax protein levels evaluation ) , ( 3 ) ISH ( detection of bcl 2 and bax mRNA ) , and ( 4 ) ISH ( TUNEL ABI [ apoptotic body index ] ) . ^^^ In CH cases , both bcl 2 and bax protein and mRNA were detected in portal and intralobular lymphocytes and in cholangiolar epithelial cells in interface areas and fibrous bands . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , apoptosis occurred in both cell lines treated with either PGZ or ATRA , which was associated with a downregulation of bcl 2 and an upregulation of bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Leukaemia cell apoptosis by beta2Mim correlated with an increase in calcium influx , decreased bcl 2 protein and mRNA levels , increase in BAX gene expression and a marked rise in BCL2 / BAX mRNA ratios . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the function of apoptosis in esophageal cancer cells induced by soybean isoflavone , and the relation between this apoptosis and expression of bcl 2 and bax . ^^^ Immunohistochemical staining and reverse transcription polymerase chain reaction were used to detect the expression of apoptosis regulated gene bcl 2 and bax . ^^^ Soybean isoflavone reduced the expression of apoptosis regulated gene bcl 2 , and improving the expression of apoptosis regulated gene bax . ^^^ This type of apoptosis might be mediated by down expression of apoptosis regulated gene bcl 2 and up expression of apoptosis regulated gene bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Early works in the area of tumor biology such as the role of p 53 , bcl 2 , bax , MEN 1 , FGF TGF PDGF and VEGF expression are of interest and need to be explored further . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Cell survival rate was measured by trypan blue , apoptosis rate by TUNEL method and the expression of bcl 2 , bax and bad by flow cytometry . ^^^ The expression of bax and bad ( especially the former ) , but not bcl 2 of SMMC 7721 cells transfected with antisense DNMT 1 gene fragment was markedly higher than those of SMMC 7721 cells transfected with sense DNMT 1 gene fragment or empty vector . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The apoptotic cells , bcl 2 and bax were detected through immunocytochemical method ( ICC ) and Tolt mediated 10 duTP nick end labeling ( TUNEL ) . ^^^ Computer image analysis system was used to quantify the expression of bcl 2 and bax by detecting the absorbance value of positive products . ^^^ As time went on , the expression of bcl 2 was decreased and in every group , with the change in absorbance value of bcl 2 significantly different from 24 to 36 hours ( P < 0 . 05 ) , whereas that of bax remained almost unchanged . ^^^ The ratio of bax / bcl 2 was increased with the increase in the concentration of MLT . ^^^ CONCLUSION : Melatonin may induce apoptosis in the hepatocarcinoma cells which is concentration and time dependent , in which bcl 2 and bax are involved . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 mRNA decreased 2 . 4 to 3 . 0 fold , whereas Bax mRNA was unchanged . ^^^ The Bax to Bcl 2 protein ratio increased by 1 . 6 fold . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Freshly separated peripheral blood lymphocytes were stained for apoptosis markers ( CD 95 , Bax , Bcl 2 , TNF receptor ) and for an activation marker ( CD 45 RO ) , and the apoptosis frequency of cells bearing these markers were assessed by the terminal deoxynucleotidyl transferase mediated dUTP digoxigenin nick end labeling method and nuclear condensation analysis with laser scanning cytometry . ^^^ In patients with RA , decreased Bax expression and decreased apoptosis rate within the Bax positive cells were found , whereas Bcl 2 expression was elevated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was assessed by TUNEL , BAX , and BCL 2 immunohistochemical assays . ^^^ Chronic alcoholics compared with controls showed a significantly higher apoptotic index in TUNEL ( 2 . 35 % + / 0 . 25 % versus 0 . 18 % + / 0 . 03 % , P < 0 . 001 ) , BAX ( 9 . 16 % + / 2 . 00 % versus 0 . 66 % + / 0 . 22 % , P < 0 . 001 ) , and BCL 2 muscle assays ( 8 . 08 % + / 0 . 20 % versus 0 . 83 % + / 0 . 20 % , P = 0 . 001 ) , respectively . ^^^ In addition , these apoptotic indexes were higher in alcoholics with skeletal myopathy compared with in those without skeletal myopathy ( 3 . 04 % + / 0 . 36 % versus 1 . 65 % + / 0 . 26 % , P = 0 . 004 for TUNEL ; 17 . 00 % + / 2 . 78 % versus 1 . 33 % + / 0 . 22 % , P < 0 . 001 for BAX ; and 15 . 13 % + / 3 . 2 % versus 1 . 03 % + / 0 . 33 % , P < 0 . 001 for BCL 2 assays , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , HLA class 1 expression was expressed more frequently in tissues with high apoptotic activity and was significantly associated with the expression of the proapoptotic bax gene ( P = 0 . 02 ) , and was inversely associated with expression of the antiapoptotic bcl 2 gene ( P = 0 . 03 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to delineate the mechanism ( s ) , glutamate induced cell death of HT 22 cells was assessed by measuring ( a ) DNA fragmentation in the presence or absence of 11 equine estrogens ( components of the drug CEE ) ; ( b ) cell death and ( c ) levels of anti apoptotic ( Bcl 2 ) and proapoptotic ( Bax ) proteins in the presence or absence of two equine estrogens , Delta 8 , 17beta E 2 and 17beta estradiol ( 17beta E 2 ) by LDH release assay and Western blot analysis respectively . ^^^ Western blot analysis indicated that glutamate also significantly decreased the levels of Bcl 2 and increased Bax levels . ^^^ This glutamate induced change in the ratio of Bcl 2 to Bax was reversed by estrogens with Delta 8 , 17beta E 2 being more potent . ^^^ CONCLUSIONS : In HT 22 mouse hippocampal cells , glutamate induced apoptosis that was associated with DNA fragmentation , morphological changes and up regulation of the pro apoptotic protein Bax and down regulation of the anti apoptotic protein Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of Bcl 2 and Bax oncoproteins in rabbit craniomandibular joint . ^^^ The purpose of this study was to elucidate the expression of proto oncogene Bcl 2 ( anti apoptotic ) and Bax ( pro apoptotic ) in fibrocartilage of the disc and hyaline cartilage of the condyle in the rabbit craniomandibular joint ( CMJ ) . ^^^ Sections were processed by the immunohistochemical techniques using mouse anti Bcl 2 and anti Bax antibodies . ^^^ The cytoplasm of chondrocytes in the disc exhibited a high intensity for Bcl 2 , while Bax activity was only sporadically observed . ^^^ We have shown that Bcl 2 and Bax proteins are present in CMJ cartilage and their expression patterns suggest that these oncoproteins are involved in chondrocyte survival or death via apoptotic pathways . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate the role of Bcl 2 in the development of glomerular lesions in human IgA nephropathy ( IgAN ) , we applied immunohistochemistry coupled with in situ hybridization to detect the expression of Bcl 2 products and their association with Bax , p 27 ( kip 1 ) , and p 57 ( kip 2 ) in modulating the apoptotic , proliferative , and sclerotic events in progressive glomerular injury . ^^^ Bcl 2 downregulation in progressive IgAN was associated with an increased Bax / Bcl 2 ratio in glomerular epithelial cells and correlated with the downregulation of high endogenous podocyte p 27 ( kip 1 ) and p 57 ( kip 2 ) expression . ^^^ Bax / Bcl 2 ratios positively correlated with glomerular cell apoptosis and the degree of glomerulosclerosis , whereas p 27 ( kip 1 ) and p 57 ( kip 2 ) expression levels were inversely correlated with mesangial hypercellularity and glomerulosclerosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pretreatment tumors ( n = 54 ) were analyzed for epidermal growth factor receptor ( EGF R ) , bax , and bcl 2 expression by immunohistochemistry and for p 53 mutations by direct DNA sequencing of exons 5 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tissue sections were examined immunohistochemically for expression of proapoptotic proteins ( Bax , p 53 ) , antiapoptotic proteins ( Bcl 2 , Mcl 1 ) , and spontaneous apoptosis ( M 30 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mcl 1 and Bcl 2 / Bax ratio are associated with treatment response but not with Rai stage in B cell chronic lymphocytic leukemia . ^^^ Although both Bcl 2 / Bax ratio and Mcl 1 have been identified to be of clinical relevance in B cell chronic lymphocytic leukemia ( CLL ) , there is controversy regarding their role ; further , their relative importance is not well delineated . ^^^ Expression of Bcl 2 , Bax , the Bcl 2 / Bax ratio , and Mcl 1 in 51 consecutive previously untreated CLL patients and 16 controls was determined by Western blotting . ^^^ The Bcl 2 / Bax ratio was significantly associated with treatment response only when CR and PR were considered together ( 0 . 89 + / 0 . 53 [ CR + PR ] vs . 3 . 38 + / 4 . 47 [ NR ] , P = 0 . 0118 ) . ^^^ Low Mcl 1 appears to be a requirement for CR , while low Bcl 2 / Bax ratio is indicative of some response to conventional treatment . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Encapsulated islet function , islet vitality , and islet cell replication were assessed , as well as the mRNA expression of Bcl 2 , Bax , inducible nitric oxide synthase , and monocyte chemoattractant protein 1 in encapsulated islets after 48 h of culture together with microcapsules with macrophage overgrowth . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Differential expression of Akt / protein kinase B , Bcl 2 and Bax proteins in human leiomyoma and myometrium . ^^^ The expression and activation of serine / threonine protein kinase , Akt , in leiomyoma and in adjacent myometrium of human uteri was studied parallel with the changes of Bcl 2 , Bax proteins , estrogen and progesterone receptors during menstrual cycle and early stage of the menopause . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of p 53 , bcl 2 , and Bax expression in early breast cancer . ^^^ The purpose of this study was to determine the prognostic value of bcl 2 , Bax , and mutant p 53 in stage 1 breast cancer . ^^^ Paraffin embedded formalin fixed tissues were immunostained with bcl 2 , Bax and p 53 monoclonal antibodies using a standard avidin biotin peroxidase reaction . ^^^ Neither bcl 2 nor Bax expression was associated statistically with disease free or overall survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic proteins in the temporal cortex in schizophrenia : high Bax / Bcl 2 ratio without caspase 3 activation . ^^^ Bcl 2 interacts with the proapoptotic Bax protein at an upstream checkpoint to regulate the activation of apoptosis by caspase 3 and other proteolytic caspase proteins . ^^^ A high Bax / Bcl 2 ratio is associated with greater vulnerability to apoptotic activation , while a high caspase 3 level is often associated with apoptotic activity . ^^^ It was hypothesized that the Bax / Bcl 2 ratio , but not caspase 3 , would be high in the temporal cortex of patients with chronic schizophrenia . ^^^ METHOD : Bax , Bcl 2 , and caspase 3 proteins were measured by semiquantitative Western blot in Brodmann ' s area 21 ( middle temporal gyrus ) of postmortem tissue from patients with schizophrenia ( N=15 ) , bipolar disorder ( N=15 ) , or major depression ( N=15 ) and nonpsychiatric comparison subjects ( N=15 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis was used to identify the expression of bcl 2 and bax . ^^^ The expression of bcl 2 was decreased and the expression of bax remained almost unchanged along with the increase of BBSKE concentration . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To begin elucidating the mechanism of genistein induced sensitization of WSU DLCL ( 2 ) cells to CHOP chemotherapy in this xenograft mouse model , we studied the in vitro effect of genistein on WSU DLCL ( 2 ) growth inhibition , cell cycle , Bax : Bcl 2 ratio , NF kappaB DNA binding , and apoptosis in vitro . ^^^ At 30 micro M , genistein inhibited the growth significantly , induced G ( 2 ) M arrest , increased Bax : Bcl 2 ratio , decreased NF kappaB DNA binding , and induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The change in the expression of Bcl 2 , Bcl 10 ( L ) , and Bax in response to hop bitter acids was studied , and the Bcl 2 protein level slightly decreased ; however , the Bcl 10 ( L ) protein level was obviously decreased , whereas the Bax protein level was dramatically increased , indicating that the control of Bcl 2 family proteins by hop bitter acids might participate in the disruption of mitochondrial integrity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in the expression levels of cyclin dependent kinase inhibitors , p 21 and p 27 , an antiapoptotic factor , Bcl 2 , and a proapoptotic factor , Bax , were also investigated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In epithelial cells , NO increased apoptosis by 2 . 1 + / 0 . 2 fold , augmented mRNA expression of Bax , and reduced expression of Bcl 2 compared with basal cultures . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Importantly , this reduction in UV mediated apoptosis was associated with downregulation of the proapoptotic Bax protein , with no effect on Bcl 2 expression , suggesting that this antiapoptotic role of p 16 is mediated via the intrinsic mitochondrial pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We screened Bcl w , Bcl 10 , and Bax ( members of Bcl 2 family ) using polymerase chain reaction and singlestrand conformation polymorphism analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of the apoptotic mediators tested , the anti apoptotic protein Bcl 10 ( L ) was strongly down regulated by combined treatment of the cells with SBHA and TRAIL but not by the HDAC inhibitor alone , while little or no change in the expression of other Bcl 2 family members highly expressed in MM cells , including Mcl 1 and Bax , was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Myocardial injury was determined by triphenyltetrazolium chloride ( TTC ) staining , terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) , bax , bcl 2 , poly ( ADP ) ribose polymerase ( PARP ) cleavage , caspase 3 , 8 , and 9 cleavage and activity , Fas ligand ( FasL ) , and Fas activated death domain ( FADD ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of p 53 , Bcl 2 , Bax , Ki 67 and PCNA protein were detected by immunohistochemical method . ^^^ Expression of p 53 protein in HCC cells increased with the increase of pathological grades ( P < 0 . 05 ) , and correlated positively with expressions of Ki 67 and PCNA protein , and negatively with Bcl 2 to Bax protein expression rate and AI ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Semi quantitative RT PCR was used to measure Bid , Bax and Bcl 2 mRNA expressions . ^^^ Expressions of Bid , Bax and Bcl 2 in resection margin tissues without H pylori infection , with cagA H pylori infection and cagA+ H pylori infection increased significantly in turn ( Bid , 0 . 377 , 0 . 686 and 0 . 939 respectively , P < 0 . 05 ; Bax , 0 . 353 , 0 . 645 and 1 . 001 respectively , P < 0 . 05 ; Bcl 2 , 0 . 371 , 0 . 487 and 0 . 619 respectively , P < 0 . 05 ) . ^^^ In H pylori negative specimens , expressions of Bid and Bax correlated negatively with that of Bcl 2 respectively in adenocarcinoma tissues ( Bid vs Bcl 2 , r= 0 . 409 , P < 0 . 05 ; Bax vs Bcl 2 , r= 0 . 451 , P < 0 . 05 ) . ^^^ In H pylori positive specimens , expressions of Bid and Bax did not correlate with that of Bcl 2 in adenocarcinoma tissues ( Bid vs Bcl 2 , r=0 . 187 , P > 0 . 05 ; Bax vs Bcl 2 , r=0 . 201 , P > 0 . 05 ) , but correlated positively with that of Bcl 2 respectively in resection margin tissues ( Bid vs Bcl 2 , r=0 . 331 , P < 0 . 05 ; Bax vs Bcl 2 , r=0 . 295 , P < 0 . 05 ) . ^^^ CONCLUSION : H pylori may enhance Bid , Bax and Bcl 2 mRNA levels and cause deregulation of these apoptosis associated genes expressions , which may play a role during development of gastric adenocarcinoma induced by H pylori . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We performed semi quantitative RT PCR to examine the expression level of bak , bax , bcl 2 and bcl xL in 70 % hepatectomized rat livers during the whole regeneration process and compared to that of the sham and normal groups . ^^^ RESULTS : The expression of bak and bax was decreased whereas that of bcl 2 and bcl xL was increased in hepatectomized animals compared to normal liver at most time points . ^^^ CONCLUSION : The expression changes of bak , bax , bcl 2 and bcl xL genes are altered not only due to regeneration , but also due to effects of surgical operations . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic value of immunohistochemical expression of p 53 , bax , Bcl 2 and Bcl xL in resected non small cell lung cancers . ^^^ METHODS AND RESULTS : We investigated the immunohistochemical expression of p 53 and Bcl 2 gene family members ( bax , Bcl 2 and Bcl xL ) in 94 non small cell lung cancer specimens to establish the role of these genes in lung cancer pathogenesis , and to evaluate their prognostic importance . ^^^ Finally , we found no correlation between frequent detection of aberrant p 53 protein and expression of either Bcl 2 , bax or Bcl xL or with patient survival time . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the apoptotic process involves activation of caspase 9 , a decrease of Bcl 2 as well as Bcl XL levels , and an increase of Bax levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of biochemical markers associated with apoptosis ( bax , bcl 2 and caspases 3 , 8 & 9 ) was investigated using immunohistochemistry . ^^^ Despite the TUNEL and EM changes , immunohistochemistry failed to detect any changes in the ratio of bax to bcl 2 in tibial chondrocytes of STR / ort mice . ^^^ Both bcl 2 and bax levels decreased with age in morphologically normal STR / ort and control CBA cartilage . ^^^ Absence of changes in bax to bcl 2 ratio in STR / ort chondrocytes indicate that the mitochondrial pathway of apoptosis is unlikely to be involved . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
O ( 6 ) MeG triggered apoptosis in proliferating lymphocytes was preceded by a wave of DSBs , which coincided with p 53 and Fas receptor upregulation , while Fas ligand , Bax and Bcl 2 expression was not altered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To identify specific genes affected by ErbB 2 mediated NF kappaB activation , a group of IR responsive elements Cyclin B 1 , Cyclin D 1 , Bcl 2 , Bcl / XL , BAD and BAX were evaluated . ^^^ Basal levels of prosurvival elements Cyclin B 1 , Cyclin D 1 , Bcl 2 and Bcl / XL but not apoptotic BAD and BAX were upregulated in MCF 7 / ErbB2 cells with striking enhancements in Bcl 2 and Bcl / XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After treatment , lesions were excised and stained for CD 20 , CD 3 , CD 4 , CD 56 , bcl 2 , bax , caspase 3 , and p 53 . ^^^ No differences were seen in either bax or bcl 2 staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax deficient and bcl 2 transgenic bone marrow cells were used to assess the importance of the mitochondria in IL 4 mediated effects . ^^^ Accordingly , IL 4 mediated apoptosis was inhibited by Bax deletion or bcl 2 overexpression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family members ( Bcl 2 , Bax , Bcl w and Bcl 10 ( L ) ) are crucial integrators of signals for cell survival and death ; the pro or antiapoptotic activities of these proteins are regulated by their subcellular localization . ^^^ Bcl 2 directly inserts into the membranes , where it acts ; however , Bax requires a stimulus dependent translocation from an inactive cytosolic to an active membrane inserted state . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Temporal changes in PTHrP , Bcl 2 , Bax , caspase , TGF beta , and FGF 2 expression following growth plate irradiation with or without radioprotectant . ^^^ Immunohistochemical staining for PTHrP , Bcl 2 , Bax , caspase 3 , FGF 2 , and TGF beta was performed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recent studies have uncovered a number of Bcl 2 related gene products that regulate apoptosis either negatively or positively , and Bcl 2 forms heterodimers with at least one of these proteins , Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Release of cytochrome c from mitochondria into the cytosol was distinct 9 h after irradiation , while the levels of most apoptosis related molecules such as Fas , FasL , Bcl 2 , Bax and p 53 were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot and immunofluorescence analysis revealed that the anti apoptotic Bcl 2 and Bcl xL levels were downregulated , together with the pro apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This requirement can be circumvented by a concomitant deletion of Bax , a pro apoptotic Bcl 2 family member , thus allowing an examination of the role of neurotrophins in axon growth independently of their function in cell survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
G110R substitution confers on Bcl 2 ( 1 ) substantially increased binding affinity to Bak , Bad and Bax BH 3 peptides , demonstrating that R 110 is a key contributor to the BH 3 binding affinity of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The extent of apoptosis was determined using TdT dUTP nick end labeling ( TUNEL ) and immunohistochemistry of apoptosis regulators caspase 3 , caspase 9 , Bax , and Bcl 2 . ^^^ Bax expression was present in TUNEL+ apoptotic cells , and Bcl 2 was rarely present in the atherosclerotic wall . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this paper we demonstrate that the SAH region in Siva 1 is sufficient to specifically interact with the anti apoptotic members of the BCL 2 family such as BCL XL and BCL 2 but not its apoptotic member BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TRAIL induced regulation of Bcl 2 family of proteins included an up regulation in Bcl 2 / Bcl XL and a down regulation in Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis modulatory activities of transiently expressed Bcl 2 : roles in cytochrome C release and Bax regulation . ^^^ Moreover , we have shown that unlike the pro apoptotic protein Bid , the toxicity associated with the transient expression of Bcl 2 occurs independent of the activity of the endogenous Bax . ^^^ Finally , we found that in spite of its intrinsic toxicity , transiently expressed Bcl 2 is fully capable of blocking the ectopically expressed Bax from localizing to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results demonstrated that high concentrations of MTBITC can also induce apoptosis , through an increase of p 53 and bax , but not bcl 2 , protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This cell death involves neither subcellular redistribution of p 53 nor transcriptional regulation of p 53 target genes such as Bax , Ras , Puma or Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of cobalt and chromium ions on bcl 2 , bax , caspase 3 , and caspase 8 expression in human U 937 macrophages . ^^^ The purpose of this study was to analyze the effect of Co ( 2+ ) and Cr ( 3+ ) ions on the expression of bcl 2 , bax , caspase 3 and caspase 8 to better understand the mechanisms leading to ion induced apoptosis in macrophages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When cells were co transfected with the GFP gene and the bcl 2 family genes bcl 2 , bcl 10 ( L ) , and bax , mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Anti apoptotic Bcl 2 and Bcl XL , pro apoptotic Bax , caspase 3 and caspase 9 expression and activation were analysed using Western immunoblots and densitometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Weak expression of other bcl 2 family member proteins , bax , bcl 2 and bcl xL , were also found in the immunoblots . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Morphological alterations of 4 , 6 diamidino 2 phenylindole ( DAPI ) labeled nuclei , DNA fragmentation patterns on agarose gels , as well as expression profiles of the apoptosis related regulatory proteins Bax and Bcl 2 showed that apoptosis was prevalent in younger animals , whereas only necrosis was detected in hippocampi of rats treated at 7 days . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the cyclin dependent kinase ( CDK ) inhibitor p21WAF1 / CIP1 was markedly reduced , but neither Bcl 2 nor Bcl xL or Bax were modulated by c Src inhibition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 protein expression were examined by histochemistry . ^^^ Bax expression was also increased in the hippocampal CA 1 region at 72 hours after seizures ( P < 0 . 001 ) , but Bcl 2 expression did not increase , while Bcl 2 / Bax ratio decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results came along with a low level of the anti apoptotic proteins Bcl 2 and Bclx ( L ) in Wmyo , whereas there was an increase in the level of the pro apoptotic molecule Bax when compared to the results obtained for Fb and Hmyo . ^^^ Hmyo showed a higher level of Bcl 2 compared to Fb but no difference in the Bax or Bclx ( L ) level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TUNEL method was adopted used to detect the apoptosis of neurons after irradiation , then immunohistochemistry was used to detect Bcl 2 , Bax expression in all brain tissue . ^^^ RESULTS : TUNEL positive rate , Bax and Bcl 2 positive cell numbers could be found in decranium + radiation group [ ( 26 . 45 + / 9 . 27 ) % , ( 23 . 5 + / 3 . 58 ) , ( 11 . 1 + / 2 . 55 ) respectively ] . ^^^ But the difference was not found in the ratio of Bax to Bcl 2 . ^^^ Bax , Bcl 2 gene participated in regulation of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA expression levels of Bcl 2 , Bax , inducible nitric oxide synthase ( iNOS ) , and monocyte chemoattractant protein 1 ( MCP 1 ) were assessed , as well as the amount of nitrite and MCP 1 in the culture medium . ^^^ Hypoxia was not associated with changes in the Bcl 2 / Bax mRNA ratio , but it did increase the expression of iNOS and MCP 1 mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Determination of the in vivo effects of cladribine alone and its combination with cyclophosphamide or cyclophosphamide and mitoxantrone on Bax and Bcl 2 protein expression in B CLL cells . ^^^ The present study investigated a correlation between expression of Bcl 2 family members , Bax and Bcl 2 ( the Bax / Bcl 2 ratio values ) in B CLL cells in vivo and the response of these cells to chemotherapy . ^^^ Western blot technique combined with videodensitometry was used for Bax and Bcl 2 determination in homogenate , nuclear and postnuclear fractions of mononuclear cells isolated from peripheral blood of B CLL patients treated with cladribine alone ( C ) , and in combination with cyclophosphamide ( CC ) or mitoxantrone and cyclophosphamide ( CMC ) . ^^^ The Bax / Bcl 2 ratio values were changed in B CLL cells originated from blood samples of patients treated by the three therapy protocols , and was the most elevated in the case of CMC treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we extended those investigations to determine whether apoptotic cell death and associated Bcl 2 and Bax proteins were modulated by tomato sauce intervention . ^^^ Tomato sauce consumption did not affect Bcl 2 expression but decreased Bax expression in carcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Paraffin embedded pre therapy biopsy specimens from these patients were used for immunostaining to evaluate the relationships between local tumor control and expression of the following previously reported predictive factors for local recurrence of head and neck cancer treated by radiotherapy : Ki 67 , Cyclin D 1 , CDC25B , VEGF , p 53 , Bax and Bcl 2 . ^^^ There were no significant associations between local control and expression of Ki 67 ( P = 0 . 467 ) , Bcl 2 ( P = 0 . 127 ) , Bax ( P = 0 . 242 ) , p 53 ( P = 0 . 262 ) , Cyclin D 1 ( P = 0 . 245 ) , CDC25B ( P = 0 . 511 ) or VEGF ( P = 0 . 496 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decreases in cellular metabolism may sensitize cells to activation and action of the pro apoptotic Bcl 2 family members , Bak and Bax , and promote apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that cytosolic localization of endogenous wild type or trans activation deficient p 53 was necessary and sufficient for apoptosis . p 53 directly activated the proapoptotic Bcl 2 protein Bax in the absence of other proteins to permeabilize mitochondria and engage the apoptotic program . p 53 also released both proapoptotic multidomain proteins and BH 3 only proteins [ Proapoptotic Bcl 2 family proteins that share only the third Bcl 2 homology domain ( BH 3 ) ] that were sequestered by Bcl xL . ^^^ We propose that when p 53 accumulates in the cytosol , it can function analogously to the BH 3 only subset of proapoptotic Bcl 2 proteins to activate Bax and trigger apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We assessed survivin expression in ovarian carcinoma , correlating results with expression of other anti apoptotic ( bcl 2 , bcl 10 , mutant p 53 ) and pro apoptotic ( bax ) markers , with prognostic parameters , and prognosis . ^^^ Paraffin embedded sections of 49 ovarian carcinoma were immunostained for survivin , bcl 2 , bcl 10 , bax , and p 53 . ^^^ Frequency of survivin , bcl 2 , bcl 10 , bax , and p 53 was 73 . 5 % , 36 . 7 % , 93 . 9 % , 77 . 6 % , and 60 . 4 % , respectively . ^^^ Survivin expression did not correlate with bcl 2 , bcl 10 , or bax expression , stage , or overall or disease free survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tea pigments inhibited the proliferating cell nuclear antigen labeling index ( PCNA LI ) , the expression of Bcl 2 protein and ras p 21 protein , and induced the expression of Bax protein in rat colorectal cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Secondly , EGR 1 protein can eliminate `` induced radiation resistance ' ' by inhibiting the functions of radiation induced pro survival genes ( NFkappaB activity and bcl 2 expression ) and activate proapoptotic genes ( such as bax ) to confer a significant radio sensitizing effect . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Molecular markers in breast cancer : can we use c erbB 2 , p 53 , bcl 2 and bax gene expression as prognostic factors . ^^^ We examined the prognostic value of c erbB 2 , p 53 , bcl 2 and bax overexpression in breast cancer . ^^^ Immunostaining for c erbB 2 , p 53 , bcl 2 and bax gene expression was performed on 121 paraffin embedded specimens of Stage 1 , 2 and 3 breast cancer patients diagnosed and treated in Hippokration Hospital , Athens Medical School , between 1986 and 1992 . ^^^ The primary tumor from 27 ( 24 . 1 % ) , 69 ( 59 % ) , 18 ( 15 % ) and 63 ( 53 . 4 % ) patients stained positively for c erbB 2 , p 53 , bcl 2 and bax gene expression , respectively . ^^^ C erbB 2 , p 53 , bcl 2 and bax genes have limited prognostic value . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression levels of p 53 , Bcl 2 , Bcl xL , Bad , Bax , survivin , Caspase 3 and poly ( ADP ribose ) polymerase ( PARP ) were evaluated by immunoblot analysis . ^^^ During the induction of apoptosis , expression of Bcl 2 , Bcl xL and survivin was decreased , and that of p 53 , Bad and Bax was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We previously demonstrated the Korean mistletoe lectin ( Viscum album var . coloratum , VCA ) induced apoptosis by down regulation of Bcl 2 and telomerase activity and by up regulation of Bax through p 53 and p 21 independent pathway in hepatoma cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Diploid human lung fibroblasts were incubated with 3 9 uM Na2CrO4 , and RNA was isolated at 4 , 8 , and 24 h , as well as 24 h after Cr ( 6 ) exposure was terminated ( recovery ) . mRNA expression was quantitated by RNase protection assay with a 32P labeled multi transcript probe containing gene sequences for the cdk inhibitors , p21waf1 / cip1 , p27kip1 , p16INK4a , p15INK4b ; the pro apoptotic proteins bcl XS and bax ; the anti apoptotic proteins bcl W , bcl XL , and bcl 2 , GADD 45 , and cyclin A . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlation of bcl 2 and bax with apoptosis in human pituitary adenomas . ^^^ Bcl 2 oncogene and Bax gene play an important role in regulating apoptosis . ^^^ In the present study , the expression of bcl 2 and bax was investigated and correlated with apoptosis in a series of 81 pituitary adenomas . ^^^ Bcl 2 and bax proteins were localized by immunohistochemistry and the histoscore ( HSC ) was assessed by multiplying the immunohistostaining grade ( 1 to 4 ) by the staining intensity grade ( 1 to 3 ) . ^^^ According to bcl 2 / bax HSC the tumors were separated in group A when > or = 1 and group B when < 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This evidence that ER induced apoptosis mediated by the mitochondrial pathway was additionally supported by the finding that levels of Bcl 2 , Bcl xl , and XIAP protein were significantly lower ( P < 0 . 01 ) , and levels of Bax and Apaf 1 were elevated ( P < 0 . 02 ) in ER carcinomas versus those carcinomas from control or ER REP rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the effect of HIV 1 gp 160 protein and morphine on murine macrophage and human monocyte apoptosis . gp 160 not only promoted murine macrophage apoptosis but also enhanced macrophage iNOS expression / NO generation . gp 160 also altered macrophage bax and bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An early ( 2 3 days post CCI ) E2F1 and p 53 independent apoptosis appeared in the spinal cord as the pro apoptotic bax gene increased ( 320 + / 19 % ) , followed by an increased expression of the anti apoptotic bcl 2 and bcl xL genes ( 60 + / 11 % and 110 + / 15 % , respectively ) 7 days from CCI . ^^^ Despite the MPEP treatment , which normalised bax / bcl 2 and bcl xL / bcl xS ratios at all times post CCI , mechanical hyperalgesia reappeared by 7 days after CCI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relative to H alone , the level of 10 rhod 1 acetoxymethyl ( AM ) labeled [ Ca ( 2+ ) ] m was increased , and the frequency of cell death ( propidium iodide ( PI ) staining ) and apoptotic cells ( terminal deoxynucleotidyl transferase ( TdT ) mediated UTP nick end labeling [ TUNEL ] ) , confirmed by Annexin 5 , were augmented at the end of Re , along with appearance of cytosolic cytochrome c , activation of caspase 3 , and increased ratio of Bax and Bcl 2 . ^^^ In contrast , the levels of [ Ca ( 2+ ) ] m and the number of PI and TUNEL positive cells were significantly reduced to a level comparable to Sham control when cariporide ( 20 micromol / l ) was administered before H and during Re , respectively , associated with a reduction in cytosolic cytochrome c , caspase 3 activity and ratio of Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using RNA protection assays we found constitutive expression of most bcl 2 members with high levels of bcl 2 , bcl w , bad , bak , bax , and the bcl 2 / bax ratio , compared to normal PBL . ^^^ Spontaneous apoptosis of B CLL cells by in vitro culture resulted in decreased bcl 2 , bcl w , bfl 1 , mcl 1 , bak , bax , and bcl 2 / bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resveratrol treatment also up regulated the Bax protein and mRNA expression in a dose dependent manner ; however , Bcl 2 and Bcl xL levels were not significantly affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 : bax was proapoptotic with imexon in C2E3 , whereas bcl 2 : bax was independent of steroid resistance , chemotherapy sensitivity , and chemotherapy resistance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell cycle perturbations and apoptosis were assessed by flow cytometry ; BAX , BCL 2 , and P 53 expression was evaluated by Western blot ; and DNA damage was assessed using the alkaline Comet assay . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neutralization of endogenous BAFF and APRIL by soluble TACI and BCMA decoy receptors attenuates the survival of NHL B cells , decreases activation of the prosurvival transcription factor NF kappaB , down regulates the antiapoptotic proteins Bcl 2 and Bcl 10 ( L ) , and up regulates the proapoptotic protein Bax . ^^^ Conversely , exposure of NHL B cells to recombinant or myeloid cell derived BAFF and APRIL attenuates apoptosis , increases NF kappaB activation , up regulates Bcl 2 and Bcl 10 ( L ) , and down regulates Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decreased Bcl 2 protein levels and increased Bax protein levels were positively correlated with elevated expression of p 53 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pro apoptotic proteins of the Bcl 2 family , Bax and Bak , exhibited conformational changes in neural precursors expanding in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By its mechanism of inhibiting protein degradation , PS 341 targets a wide range of pathways relevant to tumor progression and therapy resistance and can directly modulate expression of cyclins , p 27 ( Kip 1 ) , p 53 , nuclear factor kappaB , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the downregulation of Bcl 2 and no changes in Bax protein levels in curcumin plus radiation treated PC 3 cells , together , altered the Bcl 2 : Bax ratio and this caused the enhanced radiosensitization effect . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Mouse myocardium of ischemia / reperfusion model was observed for evaluating the activity of superoxide dismutase ( SOD ) , the level of malondialdehyde ( MDA ) , the apoptotic index of cardial myocyte and the expression of apoptosis correlation protein bcl 2 and bax . ^^^ And it could depress cardial myocyte apoptosis caused by ischemia / reperfusion through up regulating bcl 2 expression and down regulating bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Recently , p 53 has been shown to directly activate the pro apoptotic Bcl 2 protein Bax allowing for mitochondrial membrane permeabilization and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PTPC may also interact with tumor suppressor proteins ( i . e . , Bax and Bid ) , oncoprotein homologues of Bcl 2 and some viral proteins , which can regulate apoptosis induced by pore opening . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antagonism between apoptotic ( Bax / Bcl 2 ) and anti apoptotic ( IAP ) signals in human osteoblastic cells under vector averaged gravity condition . ^^^ The Bax triggers cytochrome c release from mitochondria , which can be blocked by the Bcl 2 . ^^^ The mRNA levels for Bax , Bcl 2 , XIAP , and caspase 3 genes were analyzed by semi quantitative RT PCR . ^^^ Twenty four hours after starting clinostat rotation , the ratios of Bax / Bcl 2 mRNA levels ( indicator of apoptosis ) were significantly increased to 136 % of the 1G static controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objectives of this in vivo study were to investigate the effect of zinc on : tumorogenicity in nude mice , zinc accumulation in tumor tissues , and the levels of mitochondrial membrane permeability related proteins , Bax / Bcl 2 . ^^^ The levels of Bax and Bcl 2 in the tumor tissues were determined by Western blot analyses . ^^^ The inhibitory effect of zinc appears to result from zinc induced apoptosis by regulation of mitochondrial membrane permeability related Bax / Bcl 2 proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No modifications of Bcl 2 , Bcl 10 , and Bax levels were observed , as well as no changes in Pi3K / Akt and JAK / STAT pathways that are often constitutively active in these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the chemopreventive potential of isothiocyanates , we studied proliferation , apoptosis induction and p 53 , bcl 2 and bax protein expression in Jurkat T leukemia cells by the isothiocyanate sulforaphane . ^^^ Moreover , it markedly increased p 53 and bax protein expression , and slightly affected bcl 2 expression . ^^^ Moreover , sulforaphane induced apoptosis ( and also necrosis ) , mediated by an increase in the expression of p 53 , whereas it exerted little effect on bcl 2 and bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical status of p 53 , MDM 2 , bcl 2 , bax , and ER in invasive ductal breast carcinoma in Tunisian patients . ^^^ Study of the status of its target gene expression showed that MDM 2 was overexpressed in 43 tumors ( 61 % ) , bcl 2 in 29 ( 41 % ) , and bax in only 9 ( 12 % ) . ^^^ The accumulated p 53 was significantly associated with MDM 2 positive , bcl 2 negative , and ER negative tumors ( P = 0 . 024 , P = 0 . 000027 , and P = 0 . 000008 , respectively ) , whereas with bax the correlaton was not significant . ^^^ A significantly higher fraction of p 53 positive cells was observed in ER negative SBRII SBRIII tumors than in ER positive SBRI SBRII tumors ( P = 0 . 000066 ) . bcl 2 positive tumors were significantly correlated with ER positive / SBRI SBRII tumors ( P = 0 . 007 ) , but negatively correlated with p53 / bax ( P = 0000004 ) . ^^^ Regulation of the apoptotic process is involved in IDC ; bcl 2 is associated with a good prognostic marker ( ER positive and SBRI 2 ) , whereas the regulation of bax is complex and does not necessarily correlate with the overexpression of p53 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
High glucose did not induce the expression of an antiapoptotic protein Bcl 2 , nor did it reduce a proapoptotic protein Bax , but it did inhibit a hypoxia induced downregulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We demonstrate constitutive expression of BAX in virtually all embryos at all stages of development , and variable expression of BCL 2 , BCL XL , BCL W , MCL 1 BAK , BAD , BOKL , BID , BIK , and BCL XS . ^^^ Protein expression was confirmed for BCL 2 , MCL 1 , BCL 10 , BAX , BAD , and activated caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Total 40 nasal polyps biopsies were detected the expression of Bcl 2 , Bax , Bcl xL as well as Fas protein on eosinophils by immunohistochemistry . ^^^ RESULTS : In nasal polyps , the expression of Bcl 2 , Bax , Bcl xL and Fas protein on eosinophils are 5 % ( 2 / 10 ) , 65 % ( 26 / 40 ) , 40 % ( 16 / 40 ) and 90 % ( 36 / 40 ) respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we investigated the role of Bax , a proapoptotic Bcl 2 family protein , in acute ethanol induced hepatocyte apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Low doses of Taxol enhanced Bcl 2 phosphorylation and led to its degradation observed on the background of a sustained or increasing Bax level and accumulation of survivin and 10 chromosome linked inhibitor of apoptosis . c jun NH ( 2 ) terminal kinase activation was essential for the apoptosis in anaplastic thyroid cancer cells , whereas Raf / MAPK kinase / ERK and phosphatidylinositol 3 OH kinase / Akt were likely to comprise main survival mechanisms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , the bcl6 / CD10 / MUM1 / CD138 differentiation immunophenotypic profiles were studied in relation to ( a ) the apoptotic index , ( b ) the apoptosis associated bcl 2 family proteins bcl 2 , bcl xl , bax , bak , bad and bid , ( c ) the proliferation index ( Ki 67 ) and ( d ) the cell cycle proteins cyclin A , cyclin B 1 , cyclin D 3 , cyclin E , p 53 , Rb , p 16 and p 27 in 79 cases of diffuse large B cell lymphomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : p 53 , its upstream regulators ( p 14 ( ARF ) and HDM 2 ) , and downstream effectors of the apoptotic pathway ( BAX and BCL 2 ) were studied in 118 NSCLC specimens . p 53 was analyzed by single stranded conformation polymorphism analysis covering exons 2 11 and by immunohistochemistry ( IHC ) . p 14 ( ARF ) was analyzed by methylation specific polymerase chain reaction and IHC . ^^^ BAX and BCL 2 were analyzed by IHC . ^^^ Of 118 NSCLC specimens , expression of BAX and BCL 2 expression were detected in 46 tumors ( 39 % ) and 17 tumors ( 14 % ) , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tissue oxidative stress measured by GSH / GSSG was increased in the heart with increased oxidation product of mitochondrial DNA , 8 oxo 7 , 8 dihydro 2 ' deoxyguanosine , increase of Bax , decrease of Bcl 2 , and increase of apoptotic myocytes as measured by anti single stranded DNA monoclonal antibody . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase 8 cleaves full length Bid , resulting in truncated p 15 tBid . p 15 tBid is potently apoptotic and activates the multidomain Bcl 2 protein , Bax , resulting in release of cytochrome c from mitochondria . ^^^ Furthermore , it does not require Bid to interact directly with other Bcl 2 family proteins , such as Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A contemporary view of this process is that in so called type 2 cells death receptor activated caspase 8 cleaves the Bcl 2 family member Bid , which generates a truncated Bid fragment that collaborates with Bax , another Bcl 2 relative , to promote the release of mitochondrial factors necessary for activation of executioner caspases and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis and Bcl 2 , Bax in hemangioma and vascular malformations ] . ^^^ OBJECTIVE : To study the expression of apoptosis and their regulatory genes , Bcl 2 , Bax in hemangioma and vascular malformations . ^^^ The expression of Bcl 2 , Bax and Ki 67 in endothelial cells were investigated by immunohistochemical S P method . ^^^ Bcl 2 , Bax might play an important regulatory role in apoptosis in endothelial cells of strawberry hemangioma . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the ASID peptide increased ( P < 0 . 0005 ) the number of cells stained positive for Bcl 2 , while significantly ( P < 0 . 05 ) decreasing the percentage of cells stained positive for BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The clinicopathologic parameters , flow cytometric parameters , p 53 , p 27 , bax , HER 2 / neu , and bcl 2 by immunostaining , presentation of port site implants , management of the individual patient , and long term outcome were analyzed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Stable cells that express Bax [ Bax GFP ( green fluorescent protein ) ] , a proapoptotic member of the Bcl 2 family , were also established . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ki 67 , Bcl 2 , and Bax were also examined to investigate whether CD 10 expression is associated with tumor proliferation index or factors of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Survivin , bcl 2 , bax , and bcl 10 gene expression in sentinel lymph nodes from melanoma patients . ^^^ PURPOSE : The expression of apoptosis related genes , such as survivin , bcl 2 , bcl 10 , and bax , has been evaluated by reverse transcriptase polymerase chain reaction ( RT PCR ) and by immunohistochemistry in sentinel lymph nodes ( SLNs ) from melanoma patients and then correlated to the outcome of patients . ^^^ After RNA extraction , an RT PCR followed by Southern blot hybridization was performed to detect survivin , bcl 2 , bcl 10 , and bax mRNA . bcl 2 , survivin , and bax gene expression was evaluated , whenever possible , also by immunohistochemistry at the protein level . ^^^ We did not find a significant correlation between bcl 2 , bax , and bcl 10 gene expression and outcome of patients . ^^^ CONCLUSION : Our findings show a variable expression of apoptosis related genes in SLNs of melanoma patients ; more interestingly , we found that survivin expression correlates to outcome of patients in a statistically significant way , whereas the expression of other genes , such as bcl 2 , bax , and bcl 10 , did not seem to correlate to progression of disease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic function of the interleukin 7 ( IL 7 ) receptor is related to regulation of three members of the Bcl 2 family : synthesis of Bcl 2 , phosphorylation of Bad , and cytosolic retention of Bax . ^^^ Both Box 1 and Y 449 are required to signal Bcl 2 synthesis and Bax cytosolic retention . ^^^ This suggests a sequential model in which Jak 1 , which binds to Box 1 , is first activated and then phosphorylates Y 449 , leading to Bcl 2 and Bax regulation , accounting for approximately 90 % of the survival function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Epidermal growth factor receptor 1 plasma membrane expression , assessed by immunohistochemistry on paraffin embedded tissues , was correlated with clinical parameters as well as immunohistochemical expression results of HER 2 ( c erbB 2 ) , BAX , BCL 2 , p 53 and anti Ki 67 , previously studied in the same series of patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase in A . camphorata induced apoptosis was also associated with a reduction in the levels of Bcl 2 , a potent cell death inhibitor , and an increase in those of the Bax protein , which heterodimerizes with and thereby inhibits Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The up regulation of p 27 and Bax by VHL was specific since we did not detect any changes in the level of other apoptotic factors including Fas and Bcl 2 by the expression of VHL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we revealed that various metastatic cancer cells showed consistently higher levels of antiapoptotic proteins , including Bcl 2 , nuclear factor kappaB , MDM 2 , DNA dependent protein kinase ( DNA PK ) , and epidermal growth factor receptor ( EGFR ) , and lower levels of proapoptotic proteins , including Bax and p 53 than low metastatic parental cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , anti apoptotic Bcl 2 and pro apoptotic Bax gene expression and protein levels were assessed by RT PCR and Western blot analysis , respectively . ^^^ Bcl 2 mRNA and protein levels decreased significantly at 20 hours after the onset of sepsis while Bax was not altered . ^^^ This was associated with significantly increased Bcl 2 protein levels and decreased Bax gene expression in the aortic and pulmonary tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of biomolecular markers ( Ki 67 , PCNA , Bcl 2 , BAX , BclX , VEGF ) in breast tumors . ^^^ We carried out a retrospective immunohistochemical study of Ki 67 , PCNA , Bcl 2 , BAX , BclX , and VEGF expression in tumors of two groups of breast cancer patients with favorable and unfavorable course of the disease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of apoptosis in HMJ 38 treated HL 60 cells was accompanied by an apparent increase of cytosolic cytochrome c , down regulation of Bcl 2 , up regulation of Bax and cleavage of pro caspase 9 , 3 and poly ( ADP ) ribosylpolymerase ( PARP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical techniques were used with antibodies directed against glial fibrillary acidic protein ( GFAP ) , caspase 3 , Fas , Fas ligand ( FasL ) , Bax , Bcl 2 , survivin , p 53 , extracellular signal regulated kinases ( ERK1 / 2 ) , and p 38 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We studied the apoptosis of mousecirculating lymphocytes and its mechanism induced by 2 , 4 , 6 , and 8 Gy of whole body gamma irradiation and the expression of bax and bcl 2 gene products as related to apoptosis . ^^^ The abnormal expression of bax and bcl 2 gene products in irradiated lymphocytes was closely related to apoptosis in peripheral lymphocytes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression and significance of bcl 2 and bax in each phase of the cell cycle in laryngeal carcinoma ] . ^^^ OBJECTIVE : To detect the expression of bcl 2 and bax in each phase of cell cycle in laryngeal carcinoma , and explore the relationship between the expression of bcl 2 and bax in each phase of the cell cycle and the occurrence , development and prognosis in the carcinoma of larynx . ^^^ METHODS : The immunohistochemical method , TUNEL technique and flowcytometry ( FCM ) parameter analyses were combined to detect the apoptosis and the expression of bcl 2 and bax in each phase of the cell cycle in 15 polyps of vocal cord and 387 laryngeal carcinomas . ^^^ The bcl 2 , bax expression and the apoptosis was n ' t notably related to clinical stage , clinical type and T grade . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Renal ischemia reperfusion led to extended tubular apoptosis and necrosis that were associated with peroxidative damage , high levels of proapoptotic Bax expression , and low levels of antiapoptotic Bcl 2 expression , cleavage of death substrate , poly ( ADP ribose ) polymerase ( PARP ) , and activation of a key effector of apoptosis , caspase 3 . ^^^ This effect was associated with inhibition of caspase 3 activation and PARP cleavage , upregulation of Bcl 2 , and downregulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The sequential treatment of xenografted mice with irradiation followed by TRAIL induced apoptosis through activation of caspase 3 , induction of Bax and Bak , and inhibition of Bcl 2 , and completely eradicated the established tumors with enhanced survival of nude mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of exercise training on the expression of anti apoptotic proteins HSP 72 , Bcl 2 and protein kinase B ( PKB ) , and the apoptotic proteins Bax and glycogen synthase kinase 3 ( GSK 3 ) was examined . ^^^ HSP 72 and Bcl 2 protein expression were increased in the left ventricle of exercised SHR , and associated with the concomitant increased expression of the protein Bax . ^^^ Thus , the Bcl 2 / Bax ratio was not changed by exercise training , suggesting that the anti apoptotic mechanism was effective in compensating the increase in the expression of the pro apoptotic protein Bax in the myocardium of the SHR . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We analyzed the specimens of mid distal sciatic nerve , especially the morphological changes of the nerve , the different expression levels of S 100 protein and apoptosis related proteins such as Bcl 2 , Bax , and Fas in Schwann cells . ^^^ The test group had statistical significance at 1 h and day 21 . ( 3 ) As an inhibitory gene protein of Schwann cell apoptosis , Bcl 2 positive rates in the control and test groups apparently elevated and were statistically different from the normal group . ( 4 ) As a promotive gene protein of Schwann cell apoptosis , the control and test groups expressed Bax at a high level and were statistically different from the normal group . ( 5 ) As a promotive gene protein of Schwann cell apoptosis , Fas positive rate in control group was slightly elevated , but had no statistical significance compared with the normal group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By in situ hybridisation and immunohistochemistry the mRNA changes and protein expression of Bcl 2 , Bax , p 53 , and caspase 3 p 20 subunit were observed . ^^^ The mRNA and protein expression of Bcl 2 , Bax and p 53 and the activity of caspase 3 enzyme are increased . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic effects were prevented by bongkrekic acid and low dose cyclosporin A , which stabilize the mitochondrial membrane , and were associated with no evident change of inducible nitric oxide synthase ( iNOS ) , B cell leukemia / lymphoma 2 ( Bcl 2 ) or Bcl 2 associated 10 protein ( Bax ) expression . ^^^ CONCLUSIONS : Prolonged binding to PBRs may cause human beta cells functional damage and apoptosis , a phenomenon which is prevented by stabilizing the mitochondrial membrane ; occurs without changes of iNOS , Bax and Bcl 2 mRNA expression ; and involves caspase activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Percentages of CD8+Annexin V+ ( ANX+ ) and CD8+CD95+ cells , changes in mitochondrial membrane potential and levels of expression of Bcl 2 , Bcl XL , and Bax in CD8+ T lymphocytes were measured by quantitative flow cytometry . ^^^ The Bax / Bcl 2 ratio was elevated in CD8+ T cells of patients relative to NCs ( P < 0 . 01 ) , and it correlated with the percentage of ANX+CD8+ T cells ( P = 0 . 007 ) . ^^^ Up regulated Bax and Bcl XL expression , the elevated Bax / Bcl 2 ratio and its association with ANX binding implicate the mitochondrial pathway in death of CD8+ T cells of patients with SCCHN . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antisense transfected cells expressed high levels of Bax and Bak , but low levels of Bcl 2 and Bcl XL when treated with CDDP , peplomycin , 5 fluorouracil or gamma rays . ^^^ Bcl 2 ubiquitination was increased , but ubiquitination of Bad and Bax was decreased in the antisense transfected cells , although their ubiquitination was increased by the antioxidants . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Astilbic acid induced COLO 205 cell apoptosis by regulating Bcl 2 and Bax expression and activating caspase 3 . ^^^ Expressions of Bcl 2 and Bax proteins were visioned by immunohistochemical analysis . ^^^ Meanwhile the expression of Bcl 2 protein was decreased while that of Bax was increased and relative MTP was decreased as well . ^^^ CONCLUSION : AA showed potent inhibitory activity on COLO 205 cells proliferation , and could induce COLO 205 cells apoptosis through disturbing DNA replication , down regulating Bcl 2 expression , and up regulating Bax expression , lowering relative MTP , and activating caspase 3 pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After emodin treatment , there was a down regulation of Cyclin B 1 , P34cdc2 , and Bcl 2 protein expression while the Bax protein expression was unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with this , the activated PKCdelta catalytic domain triggered the redistribution and activation of Bax , a Bcl 2 family protein that can directly induce cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR was used to examine the effect of ZLJ on expressions of prostate marker gene ( PSA ) , androgen receptor ( AR ) , apoptosis related genes ( bcl 2 and bax ) , and Western blot assay was used to detect the effect on protein expression of bcl 2 and bax . ^^^ ZLJ could down regulate the expression of PSA , AR , bcl 2 genes and lower bcl 2 protein expression , but showed ineffective on bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The parameters reflecting cell proliferation and cell death were studied together with oxidative response , mitochondrial membrane potential ( MMP ) and changes of selected regulatory molecules associated with cell cycle ( p 27 ( Kip 1 ) and p 21 ( Cip1 / WAF1 ) ) and apoptosis ( caspase 3 , caspase 9 , poly ( ADP ribose ) polymerase PARP , Bcl 2 , Bax , Bak , Mcl 1 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of platelet derived growth factor and interleukin 10 on Fas / Fas ligand and Bcl 2 / Bax mRNA expression in rat hepatic stellate cells in vitro . ^^^ AIM : To investigate the effects of platelet derived growth factor ( PDGF ) and interleukin 10 ( IL 10 ) on Fas / Fas ligand and Bcl 2 / Bax mRNA expressions in rat hepatic stellate cells . ^^^ Semi quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) analysis was employed to compare the mRNA expression levels of Fas / FasL and Bcl 2 / Bax in HSCs of each group . ^^^ Contrast to the control group , after treated with PDGF and IL 10 , either alone or in combination , Bcl 2 mRNA expression was down regulated and Bax mRNA expression was up regulated , both following the turn from group P , group 1 to group C . ^^^ IL 10 may promote the apoptosis of HSCs by up regulating the expressions of FasL and Bax and down regulating the expression of Bcl 2 , which may be involved in its antifibrosis mechanism . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analyses were performed on the tissues to see if they contained the apoptosis related proteins p 53 , bcl 2 , bax , and caspase 3 . ^^^ In the PIZ significant increases of bax ( 18 fold ; p < 0 . 005 ) and caspase 3 ( 20 fold ; p < 0 . 005 ) were recorded , whereas bcl 2 was upregulated in only 14 patients ( 48 . 3 % ; 2 . 9 fold increase ) compared with control tissue . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The change in expression of apoptotic markers ( Bcl 2 and Bax proteins ) brought about by various chemotherapeutic regimens is being used to identify drug resistance in the tumor cells . ^^^ METHODS : 50 cases of locally advanced breast cancer after complete routine and metastatic work up were subjected to trucut biopsy and the tissue evaluated immunohistochemically for apoptotic markers ( bcl 2 / bax ratio ) . ^^^ Modified radical mastectomy was performed in all patients three weeks after the last cycle and the specimen were re evaluated for any change in the bcl 2 / bax ratio . ^^^ RESULTS : There was a statistically significant correlation observed between clinical , immunohistochemical response ( bcl 2 / bax ratio ) and the drug induced toxicity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , increased expression of the pro apoptotic Bcl 2 family member , Bax , was also observed in AOM induced tumors in rats fed by lactoferrin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we demonstrate that antiapoptotic BCL 2 family proteins promoted tumor formation of transformed baby mouse kidney ( BMK ) epithelial cells by antagonizing BAX and BAK dependent apoptosis . ^^^ An in vitro ischemia system mimicked the tumor microenvironment , and gain of BCL 2 or loss of BAX and BAK was sufficient to confer resistance to apoptosis and to allow for accumulation of polyploid cells in vitro . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast to reported observations in acute promyelocytic leukemia , myeloma cell apoptosis was not associated with either the downregulation of Bcl 2 protein or with alterations in the expression of other Bcl 2 family members , Bax , Bak , Bag , and Bcl xl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic regulatory proteins : BCL 2 , BAX and p 27 were analyzed by immunohistochemistry and evaluated for expression in four distinct histologic patterns : hyperplastic epithelium , nodules , dilated glands and atrophic / inflammatory glands . ^^^ RESULTS : Particularly striking was the decreased expression of BAX and an abnormal BCL 2 : BAX ratio within all nodules relative to expression in other epithelial patterns . p 27 expression was decreased in 35 % of the hyperplastic epithelial areas and 10 % of the nodules . ^^^ DISCUSSION : Overall , abnormal expression of BCL 2 , BAX and / or p 27 was identified in the hyperplastic epithelium of 19 ( 90 % ) of specimens and all 12 ( 100 % ) of the hyperplastic nodules . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nimodipine modulates Bcl 2 and Bax mRNA expression after cerebral ischemia . ^^^ In order to explore whether the member of Bcl 2 gene family , for example , Bcl 2 and Bax , are induced after cerebral ischemia , and whether expression of genes can be modulated by calcium antagonist , the rat cerebral ischemic models were made by occluding left middle cerebral artery . ^^^ The expression of Bcl 2 and Bax mRNA was measured by RT PCR method . ^^^ After middle cerebral artery occlusion ( MCAO ) , the expression of both Bcl 2 and Bax mRNA were induced . ^^^ Level of Bcl 2 mRNA increased steadily and level of Bax mRNA increased gradually at first , reached a peak after 24 h , then decreased slowly . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immunohistochemistry was used to detect the expression of Bcl 2 , Bax and IL 6 proteins in atherosclerotic plaques . ^^^ Immunohistochemistry revealed that the expression of Bcl 2 , Bax and TL 6 in model group was significantly higher than in normal group . ^^^ In the SM group , the expression of Bcl 2 protein was up regulated and that of Bax was down regulated . ^^^ To decrease the expression of Bax and increase the expression of Bcl 2 protein may be one of the mechanisms of SM against atherosclerosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , patterns of cytoplasmic intermediate filaments ( cytokeratin 18 ) , actin filaments , and microtubules , as well as Bax and Bcl 2 proteins in human bladder carcinoma T 24 cells were examined before and after retinoic acid treatment by immunocytochemistry and conventional electron microscopy . ^^^ Our results demonstrate that the redistribution of Bax and Bcl 2 proteins in the subcellular compartment of T 24 cells is correlated with reorganization of the cytoplasmic intermediate filament network and that cytokeratins are cleaved by caspases , as revealed by the M 30 antibody which recognizes a specific caspase cleavage site within cytokeratin 18 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Four hours after cerebral ischaemia the brains were removed and the expression of apoptosis regulating proteins ( Bax , Bcl 2 , p 53 , Mdm 2 ) was determined using immunofluorescence and Western blot analyses . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Data are described on a retrospective immunohistochemical study of the Ki 67 , PCNA , Bcl 2 , BAX , BclX and VEGF expression in tumors of two groups of patients with breast cancer ( BC ) and with the favorable and unfavorable clinical courses . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , pneumococcus infected human alveolar epithelial cells showed Bid cleavage and reduced levels of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We now report that activation of both Bax and Bak , two proapoptotic members of the Bcl 2 family that regulate mitochondrial cytochrome c release , was inhibited in chlamydia infected cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax was up regulated and translocated to mitochondria at the IC ( 50 ) and IC ( 70 ) concentrations , whereas Bcl 2 was phosphorylated only at the highest vinflunine concentration examined ( 500 nM ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alterations in the cellular distribution of bcl 2 , bcl 10 and bax in the adult rat substantia nigra following striatal 6 hydroxydopamine lesions . ^^^ In this study we examined the localization of the pro apoptotic protein bax , and the anti apoptotic proteins bcl 2 and bcl 10 ( L ) in the substantia nigra ( SN ) of the adult rat and their response to oxidative stress caused by striatal injections of 6 hydroxydopamine ( 6 OHDA ) . ^^^ Our data show that bcl 2 , bcl 10 and bax proteins are present in the SN . ^^^ Bcl 2 and bax are localized primarily in neurons including all those positive for tyrosine hydroxylase ( TH ) . ^^^ The intraneuronal distribution of bcl 2 and bax were different . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our objective was to determine the effects of uteroplacental insufficiency upon small intestine growth , histology , gene expression of the apoptosis related proteins Bcl 2 , Bax and p 53 , and caspase 3 activity . ^^^ In association with these changes , IUGR intestinal Bcl 2 mRNA levels were decreased significantly , and Bax and p 53 mRNA were significantly increased in distal ileum . ^^^ Immunohistochemistry for Bcl 2 , Bax , and p 53 revealed similar findings . ^^^ In association with the decreased Bcl 2 and the increased Bax gene expression , increased caspase 3 activity characterized the IUGR distal ileum . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have previously reported that I3C and DIM induce apoptosis in prostate cancer cell lines through p 53 , bax , bcl 2 and fasL independent pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The inhibition of P 4 action by OP increased the expression of Fas mRNA ( P < 0 . 01 ) ; however , it did not affect bax or bcl 2 mRNA expression ( P > 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The IHS markers demonstrated positive staining with Bax p 53 , CD 40 and CD40L in AR treated cells and negative staining with Bcl 2 . 5 FU treated cells demonstrated a profile similar to AR but with less intensity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Opposite role of Bax and BCL 2 in the anti tumoral responses of the immune system . ^^^ METHODS : The rat glioma cell line A15A5 was stably transfected with human Bcl 2 and Bax transgenes and the viability of theses cell lines was analyzed in vitro and in vivo . ^^^ CONCLUSIONS : We show that the expression of Bax and Bcl 2 controls the sensitivity of the cancer cells toward the immune system . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , nicotine treatment up regulated the mRNA and protein expression of apoptosis related factors including bcl 2 mRNA and protein , but down regulated the expression of bax mRNA and protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 family member proteins Bcl xL , Bcl 2 , and Bax were assessed in glioma cells both before and after chemotherapy treatment . ^^^ Down regulation of Bcl xL and Bcl 2 resulted in reversal of the ratio of Bax / Bcl xL and Bax / Bcl 2 and enhanced cell death after treatment with BCNU . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting was used for caspase 3 and PARP , caspase 7 , caspase 9 , cytochrome c , Bcl 2 , Bax , Mcl 1 , cyclinA , cyclin B 1 , cyclin D 1 , cyclin E , CDK 2 , CDK 4 , CDK 6 , P 21 , P 27 , GADD 45 , GADD 153 . ^^^ The anti apoptosis proteins Bcl 2 and Mcl 1 were decreased in parallel and Bax increased , indicating that Bcl 2 family proteins mitochondria caspase cascade was responsible for oil induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Central mediators of delayed neuronal cell death are two families of molecules : a group of cysteine aspartate proteases , called caspases , and molecules of the bcl 2 family , e . g . bcl 2 , bax , and bid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regarding the antiapoptotic mechanisms , we have previously demonstrated that under staurosporine treatment , HalphaA and HalphaB crystallins can interact with Bax and Bcl XS , proapoptotic members of the Bcl 2 family , to sequester their translocation into mitochondria , and thus prevent the staurosporine induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , release of cytochrome c from mitochondria , down expression of Bcl 2 and Bcl 10 ( L ) , up regulation of Bax , and caspase 9 activities were observed in SM treated A 549 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that IGF 2 decreased Bcl 2 protein levels , but not Bax , resulting in a significant reduction in the Bcl 2 / Bax ratio . ^^^ Western blot analysis of Bcl 2 and Bax levels demonstrated a transient decrease in the Bcl 2 / Bax ratio at 24 h with no decrease in the ratio at 48 or 72 h . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It is suggested that increase in cell viability by KR 31378 is ascribed to the maxi K channel opening coupled upregulation of CK2 / Akt / CREB phosphorylation and downregulation of PTEN phosphorylation in association with increased Bcl 2 and decreased Bax levels . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with these findings , apoptosis induced by phenylurea based compounds was not altered by genetic alterations in the expression of Bcl 2 family proteins that control mitochondria dependent cell death pathways , including over expression of anti apoptotic proteins Bcl 2 or Bcl 10 ( L ) and genetic ablation of pro apoptotic proteins Bax and Bak . ^^^ Altogether , these findings indicate that phenylurea based XIAP antagonists block interaction of downstream effector caspases with XIAP , thus inducing apoptosis of tumor cell lines through a caspase dependent , Bcl 2 / Bax independent mechanism . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hepatocyte apoptosis and the expression of Bcl 2 and Bax in Yin jaundice rats ] . ^^^ OBJECTIVE : To investigate the effects of Yinchen Shufu Decoction on hepatocyte apoptosis and the expression of its apoptosis regulating gene Bcl 2 and Bax in Yin jaundice rats . ^^^ The TUNEL assay and the immunohistochemistry assay were used to detect the apoptosis of hepatocytes and the expression of Bcl 2 and Bax in hepatocytes respectively . ^^^ RESULTS : The rate of apoptosis cells in Yin jaundice model group was higher significantly than that in Yang jaundice model group and normal control group ( P < 0 . 01 ) , the expression of Bcl 2 in Yinchen Shufu Decoction treatment group was higher significantly than that in Yin jaundice model group ( P < 0 . 01 or P < 0 . 05 ) , and the expression of Bax in it was lower significantly than that in Ying jaundice model group ( P < 0 . 01 or P < 0 . 05 ) . ^^^ CONCLUSION : Yinchen Shufu Decoction can prevent hepatocyte apoptosis perhaps by up regulating the expression of Bcl 2 and down regulating the expression of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dynamic changes of the anti and pro apoptotic proteins Bcl w , Bcl 2 , and Bax with Smac / Diablo mitochondrial release after photothrombotic ring stroke in rats . ^^^ The anti apoptotic proteins Bcl w and Bcl 2 and the pro apoptotic protein Bax may mediate cell death or survival via regulation of the mitochondria including second mitochondria derived activator of caspase ( Smac ) / direct inhibitor of apoptosis protein ( IAP ) binding protein with low pI ( DIABLO ) release . ^^^ This study aimed to explore alterations in Bcl w , Bcl 2 , and Bax and the relationship between these proteins and Smac / DIABLO by means of in situ hybridization , immunohistochemical ( IHC ) staining , and Western blots after low and high intensity photothrombotic ring stroke . ^^^ A prolonged elevation of Bcl 2 with relatively unchanged Bax in the mitochondrial fraction was demonstrated from 4 to 72 h . ^^^ After high intensity irradiation , we observed decreased bcl w and bcl 2 mRNA with increased Bcl 2 protein in the cytosolic fraction , whereas the Bax protein remained in scattered ischaemic cells in the ring lesion and the region at risk that corresponded with release of Smac / DIABLO from mitochondria to the cytosol at 1 24 h . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We asked whether Bax , a member of the Bcl 2 family of proteins that is required for cell death in many developing neurons , might be essential for sex differences in neuron number . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in expression of p 53 , Bcl 2 , Bax , and Bak were quantified by Western immunoblotting . ^^^ Apoptosis in both phenotypes occurred with increased expression of p 53 and Bak , but no change in Bcl 2 or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Acetyl 12 , 13 epoxyl 9 trichothecene 3 , 15 diol from Isaria japonica mediates apoptosis of rat bladder carcinoma NBT 2 cells by decreasing anti apoptotic Bcl 2 expression and increasing pro apoptotic Bax expression . ^^^ AETD treatment also decreased the expression of the anti apoptotic protein Bcl 2 and increased the expression of the pro apoptotic protein Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Serum level of cholylglycine ( CG ) in 30 cases with ICP ( ICP group ) and 27 normal pregnant women ( control group ) was examined by radio immunoassay before delivery . bax and bcl 2 level in placenta was detected by immunohistochemistry method . ^^^ A significant positive correlation between CG level and bax expression level and a negative correlation between CG and bcl 2 were found in ICP group ( P < 0 . 005 , P < 0 . 005 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A panel of antibodies was used to label leukocyte antigens ( CD 68 and CD 3 ) cytokines ( IL 1alpha and IL 1beta ) and apoptosis inhibiting and promoting proteins ( bcl 2 and bax ) by means of immunohistochemical techniques . ^^^ Immunostaining with bcl 2 and bax antibodies revealed that both of these proteins were expressed in the cytoplasm of the cells in the interface membrane but they showed different cellular patterns . ^^^ Bcl 2 was localized in a small number of lymphocyte like cells while bax was expressed by large numbers of cells , mainly macrophages . ^^^ The number of cells which expressed bcl 2 was significantly lower than that of bax ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic significance of Bax , Bcl 2 , and p 53 expressions in cervical squamous cell carcinoma treated by radiotherapy . ^^^ OBJECTIVE : The objective was to determine the prognostic significance of the immunohistochemical expressions of Bax , Bcl 2 , and p 53 in squamous cell carcinoma of the uterine cervix treated by radiotherapy alone . ^^^ METHODS : One hundred and seventy four cases of squamous cell carcinoma of the cervix ( stage Ib IVa ) diagnosed between January 1996 and December 1998 were investigated for Bax , Bcl 2 , and p 53 expressions and were correlated to the patients ' survival . ^^^ Bax , Bcl 2 , and p 53 expressions were seen in 68 . 4 % , 25 . 9 % , and 77 . 6 % of cases , respectively . ^^^ In multivariate analysis by Cox ' s regression , age , stage , Bax , and Bcl 2 expressions appeared to be independent prognostic predictors of DFS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This effect was found to correlate with the up regulation of Fas / APO 1 , Fas ligand , and Bax , and down regulation of NF kappaB , Bcl 2 , and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis of bcl 2 , Bax and Bak expression in thyroid glands from patients with Graves ' disease . ^^^ In order to clarify the role of apoptosis and the expression of Bcl 2 family proteins in the pathology of Graves ' disease ( GD ) , we evaluated the apoptosis by in situ end labeling of fragmented DNA and the expression of Bcl 2 , Bax and Bak by immunohistochemistry in thyroid tissues from 20 patients with GD and in normal thyroid tissues from 6 patients with follicular adenoma ( N ) . ^^^ In lymphoid follicles Bcl 2 was expressed in the mantle zone , while Bax and Bak were both expressed in the germinal center . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These effects , associated with an up regulation of p 53 , p 21 and bax , a shuttling of p 53 protein into the nucleus and a down regulation of bcl 2 , survivin and p 27 protein , were reversed by the simultaneous addition of guanine or guanosine and were more evident using nondialysed serum containing hypoxanthine . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In parallel cultures , there was a significant increase in Bad and Bax expression , concomitant with an increase in DNA fragmentation , and a significant decrease in Bcl 2 and Bcl XL expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IFN alpha acts by delaying entry of cells into the G 1 phase of the cell cycle , as well as by increasing Bcl 2 and limiting Bax activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have used TRAIL resistant leukemic cells that are deficient in both Bax and Bak to determine the roles of these Bcl 2 members in TRAIL mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was documented flow cytometrically by monitoring the expression of bcl 2 and bax , forward light scatter , fluorescence intensity of binding of CD 45 antibody , and the binding of annexin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Identification and characterization of their substrates indicate that they regulate the turnover of key cell cycle proteins ( p27Kip1 , p21Cip1 , p57Kip2 , cyclin E ) , tumor suppressor proteins ( p 53 , RB ) , signaling kinases ( Src , Zap 70 , PI 3 kinase ) , apoptosis regulators ( Bcl 2 , Bax , Bik ) and transcription factors ( Myc , NF kappaB , E1F1 ) , all of which have been implicated in the pathogenesis of malignant lymphoma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using indirect inmunohistochemical method , the expression of Bcl XL and Bax , anti and proapoptotic proteins of Bcl 2 family , as well as of cytokine IL 1beta were studied to demonstrate the role of these substances in apoptosis regulation of sensory neurons of different subpopulations after the severance of their peripheral processes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In cervical cancer , increased bcl 2 and Bax immunoreactivity is generally associated with a better prognosis . ^^^ The purpose of this study was to evaluate the value of HPV 16 and HPV 18 typing and p 53 codon polymorphism genotyping by polymerase chain reaction and ER , PR , bcl 2 , and Bax expression by immunohistochemistry in predicting the CIN 3 clinical behavior of CIN 3 lesions . ^^^ The ER , PR , bcl 2 , and Bax immunoreactivity was scored as positive ( > 10 % staining cells ) and negative ( < 10 % staining cells ) . ^^^ The ER , PR , bcl 2 , and Bax immunohistochemical expression was stronger and more frequent in the CIN 3 group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The nasal mucusa thus obtained from two groups were studied by HE , in situ hybridization histochemical staining of Bcl 2 mRNA and immunohistochemical staining of bcl 2 / bax protein . ^^^ RESULT : The positive expression of Bcl 2 mRNA and Bcl 2 / bax protein was presented in the glandular cells , epithelium cells and eosinophils . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Change of Bcl 2 , Bax proteins and mitochondrial membrane protein on nitric oxide induced apoptosis in HL 60 cells ] . ^^^ To investigate the possible mechanisms of nitric oxide ( NO ) induced apoptosis in leukemia cell line HL 60 , HL 60 cells in vitro were incubated with sodium nitroprusside ( SNP ) , the in situ cell apoptosis quantitatively was assayed by TdT mediated dUTP nick end labeling ( TUNEL ) , the cell cycle DNA and proteins expression of Bcl 2 , Bax , mitochondrial membrane protein ( APO2 . 7 ) were analyzed by flow cytometry . ^^^ During the apoptosis process , it showed a decrease of Bcl 2 protein and an increase of Bax protein and mitochondrial membrane protein in HL 60 cell , proteins of Bcl 2 , Bax and mitochondrial membrane were expressed in a dosage and time dependent manner too . ^^^ In conclusion , during the process of SNP induced apoptosis in HL 60 cell , the expression of mitochondrial membrane protein was increased , Bcl 2 and Bax proteins may be important regulators . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Glutamate mediates cell death and increases the Bax to Bcl 2 ratio in a differentiated neuronal cell line . ^^^ Recent evidence has shown that exposure to excitatory amino acids regulates the expression of the antiapoptotic protein , Bcl 2 , and the proapoptotic protein , Bax , in neurons . ^^^ Since it has been suggested that the ratio of Bax to Bcl 2 is an important determinant of neuronal survival , the reciprocal regulation of these Bcl 2 family proteins may play a role in the neurotoxicity mediated by glutamate . ^^^ Immunoblot analysis was used to determine whether the expression of Bcl 2 or Bax was modulated during the differentiation process . ^^^ Bcl 2 protein levels were increased during maturation while Bax expression remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : With methylene chloride fraction of Herba Scutellariae barbatae ( MCSB ) , apoptosis related experiments were carried out on human U 937 leukemia cells by ( a ) 2 , 3 bis [ 2 4 nitro 5 sulphophenyl ] 2H tetrazolium 5 carboxanilide ( XTT ) assay for cytotoxicity ; ( b ) terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) assay for morphological changes ; ( c ) cell cycle analysis ; ( d ) Western blot analysis of poly ( ADP ribose ) polymerase ( PARP ) , caspase 8 , caspase 9 , caspase 3 and Bax , Bcl 2 and cytochrome c expressions for apoptosis signaling pathway . ^^^ Similarly , MCSB effectively cleaved PARP , increased the ratio of Bax / Bcl 2 and released the cytochrome c from mitochondria during apoptosis in U 937 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Also , increased expression of the pro apoptotic Bcl 2 members Bax , Bad and activation of caspase 3 and caspase 9 , but not the activation of caspase 8 or Fas were detected in the NS 1 transfected cells . p 53 induced Bax expression and subsequent activation of caspase 9 is probably the apoptotic pathway in NS 1 transfected cells since activation of the caspase 9 was suppressed by the p 53 inhibitor and apoptosis was significantly inhibited by the caspase 9 inhibitor . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We measured apoptotic changes by in situ DNA nick end labeling ( TUNEL ) , DNA fragmentation assay , and Western blotting for Bcl 2 and BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and bcl 2 imbalance and HPB infection in penile tumors and adjacent tissues ] . ^^^ OBJECTIVES : To analyze the expression pattern of apoptotic related proteins Bax and Bcl 2 and HPV virus infection in samples of penile carcinomas and adjacent tissues . ^^^ MATERIAL AND METHODS : Bax and Bcl 2 were assayed by Western Blot and HPV virus presence by PCR in 16 tumor samples , 11 adjacent skin and 10 adjacent corpus cavernosum samples . ^^^ RESULTS : Comparative to control samples , tumors showed an imbalance in Bax and Bcl 2 expression . ^^^ CONCLUSIONS : Bax and Bcl 2 imbalance is probably associated with the neoplastic process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : P gp , MRP , LRP , Bcl 2 , and Bax proteins play an important role in the multidrug resistance of leukemic cells . ^^^ P gp , MRP , and LRP proteins decrease the intracellular drug concentration , Bcl 2 and Bax proteins influence the apoptosis process . ^^^ METHODS AND RESULTS : With use of flow cytometry we measured the expression of P gp , MRP , LRP , Bcl 2 , and Bax proteins in acute myeloid leukemia patients cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Other molecular features modified by hdm 2 siRNA included decreased Bcl 2 , NF kappaB , survivin , Ras and Raf levels , elevated p 53 , p 21 , BRCA 1 , Bax , and caspase levels as well as altered expression of other genes . hdm 2 siRNA also caused cell cycle arrest at G 1 phases with reduction in cyclin and Cdk proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypoxia induced renal epithelial cell death through caspase dependent pathway : role of Bcl 2 , Bcl xL and Bax in tubular injury . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the MSI H tumors with p 53 overexpression , no expression of BAX protein was found , and there was high expression of bcl 2 protein , resulting in a low BAX / bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of PUMA is associated with the activation and clustering of the pro apoptotic Bcl 2 family member Bax and the loss of cytochrome C from the mitochondria . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bcl 2 , subunits of the respiratory chain , a subunit of the phosphatase PP2A , phospholipase PLC beta 4 and IP 3 receptor ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of important proapoptotic ( Bcl xs , Bax , p 53 , caspase 3 ) and antiapoptotic genes ( Bcl 2 and Bcl xL ) was also altered by tumor cachexia . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this investigation , we used human 1321N1 astrocytoma cells expressing a recombinant P2Y2 receptor to assess the role of this receptor in the regulation of anti apoptotic ( bcl 2 and bcl xl ) and pro apoptotic ( bax ) gene expression . ^^^ Acute treatment with the P2Y2 receptor agonist UTP up regulated bcl 2 and bcl xl , and down regulated bax , gene expression . ^^^ Activation of P2Y2 receptors induced the phosphorylation of the pro apoptotic factor Bad and caused a reduction in bax / bcl 2 mRNA expression ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objective of this study was to clarify the impact of HIF 1alpha , human papillomavirus ( HPV ) , and other molecular factors , such as p 53 , bax , bcl 2 , and their correlations on the outcome of patients with Stage IIIB cervical carcinoma in radiation therapy . ^^^ The tumor expressions of HIF 1alpha , p 53 , bax , and bcl 2 were examined by immunohistochemical staining of the pretreatment paraffin embedded specimens . ^^^ RESULTS : Of 38 patients , high expression of HIF 1alpha , p 53 , bax , and bcl 2 were seen in 17 ( 45 % ) , 22 ( 58 % ) , 15 ( 39 % ) , and 15 ( 39 % ) patients , respectively , and 28 patients ( 74 % ) showed positive infection with HPV . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Material from Group 1 resulted in an increase in the bax expression compared to Groups 2 and 3 ( P < 0 . 001 ) , while no significant differences were observed in the expression of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The morphologic changes , the apoptosis cell cycles , the mitochondrion membrane potential changes , and the Bcl 2 and Bax gene expression were observed , and the effects of Bugu Mixture on the course of cell apoptosis were evaluated . ^^^ The expression of Bcl 2 was higher in the Bugu Mixture group than that in the all trans retinoic acid ( ATRA ) induced group , and the expression of Bax was lower in the Bugu Mixture group than that in the ATRA induced group . ^^^ By preventing the transferring of the Bax protein from cell plasma to mitochondrion membrane , it takes the advantage of Bcl 2 in forming Bcl 2 / Bax homodimer so as to prevent the opening of the permeability transition pore to avoid the changing of mitochondrion membrane potential and the destruction of biosynthesis caused by the mitochondrion release of apoptosis inducing factors and to reach the objective of restraining apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , our data suggest that these processes are independent of the activity of Bax and Bcl 2 but are mediated by nitric oxide synthase . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , upon cDDP treatment , the levels of Fas , Bax and Bid remained unchanged , whereas Bcl 2 and p Bad were reduced at late times ( 120 hr ) after treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PrPc could also suppress adriamycin induced apoptosis and alter the expression of Bcl 2 and Bax , which might be another pathway contributing to PrPc related MDR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , we show that Bcl 2 overexpression , but not the pan caspase inhibitor zVAD fmk , blocks Bax conformational change and its subsequent translocation downstream of p 38 MAPK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Indeed , 1 , 25 ( OH ) 2D3 treatment increased levels of the anti apoptotic protein Bcl 2 and decreased levels of the pro apoptotic proteins Bax and Bad in a time and dose dependent way . ^^^ In conclusion , 1 , 25 ( OH ) 2D3 clearly protects keratinocytes against apoptosis ( 1 ) by activating the MEK / ERK and the PI 3K / Akt survival pathways and ( 2 ) by increasing the Bcl 2 to Bax and Bad ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The amount of apoptosis related proteins Bax , p 53 , Bcl 2 , and Mdm 2 and neurons positive for activated caspase 3 were analyzed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cadmium induces apoptotic cell death in WI 38 cells via caspase dependent Bid cleavage and calpain mediated mitochondrial Bax cleavage by Bcl 2 independent pathway . ^^^ Moreover , we found that the function of Bid and Bax was not dependent of Bcl 2 , and that ROS can also contribute in the Cd induced cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we found that shikonin induced apoptotic cell death was accompanied by upregulation of p 27 , p 53 , and Bad and down regulation of Bcl 2 and Bcl 10 ( L ) , while shikonin had little effect on the levels of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Juvenile rats with spinal cord injury also showed a reduced Bax : Bcl 2 ratio ( 4 : 1 vs . 6 : 1 ) , reduced caspase 3 staining , reduced myelin loss ( 3 % vs . 18 % ) , and less neuronal DNA damage , as compared to older rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Polychlorinated biphenyl mixtures ( Aroclors ) induce apoptosis via Bcl 2 , Bax and caspase 3 proteins in neuronal cell cultures . ^^^ Also , Bcl 2 and Bax protein were assessed in order to elucidate the cell death machinery induced in cortical neuronal cell cultures by Aroclor 1248 . ^^^ The results indicate that the increase in Aroclor induced apoptosis correlates with a reduction in the expression of antiapoptotic Bcl 2 and an increase in the expression of proapoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HUVECs in vitro expressed Bcl 2 , Bcl xL , and Bax ; however , expression was not changed by SNAP treatment in the presence or absence of LLL CHO , etoposide , or C 2 ceramide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
During the process , the expression of Bcl 2 protein was down regulated , while that of Bax and p27kip proteins was up regulated , and the activity of caspase 3 was elevated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pro apoptotic protein Fas , FasL , Bax and the anti apoptotic protein Bcl 2 , Bcl 10 ( L ) , Bcl 2 alpha were studied immunohistochemically by SP method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax was increased in the cells treated with ginsenoside Rh 2 , whereas Bcl 2 expression was not altered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After various concentration of NP , BisA and DBP treatment for 72h , the cells were harvested and detected mRNA and protein expression of PCNA , bcl 2 and bax by reverse transcription polymerase chain reaction and immunohistochemistry , respectively . ^^^ RESULTS : 32 10 10 ( 7 ) mol / L NP and 32 10 10 ( 7 ) mol / L BisA could significantly up regulate PCNA and bcl 2 mRNA expression and down regulate the bax mRNA expression , and 32 10 10 ( 6 ) mol / L DBP could up regulate PCNA mRNA expression , but had no effect on bax and bcl 2 mRNA expression . ^^^ CONCLUSION : PCNA , bcl 2 and bax pathway might involve in cell proliferation and apoptosis events by environmental estrogens in ovarian cancer PEO 4 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related proteins bcl 2 and bax along with transforming growth factor ( TGF beta 1 ) in the kidney of patients with glomerulonephritides . ^^^ Bcl 2 and bax are proteins related to apoptotic process that either provides a survival advantage to rapidly proliferating cells ( bcl 2 ) or promote cell death by apoptosis ( bax ) . ^^^ This study investigates the expression of bcl 2 and bax and the presence of apoptotic bodies in relation to the TGF beta 1 expression at the time of diagnosis in the renal biopsies of patients with glomerulonephritis ( GN ) . ^^^ Bcl 2 , bax and Transforming Growth Factor ( TGF beta 1 ) positive cells were detected in kidney biopsies by immunohistochemistry , while apoptotic cells were detected by in situ end labeling of fragmented DNA ( ISEL ) . ^^^ RESULTS : The intensity of bcl 2 , bax and TGF beta 1 immunostaining in the renal tissue of patients with GN was significantly more to the observed in the control biopsies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the role of brain derived neurotrophic factor ( BDNF ) in differentiation of cranial sensory neurons in vivo , we analyzed development of nodose ( NG ) , petrosal ( PG ) , and vestibular ( VG ) ganglion cells in genetically engineered mice carrying null mutations in the genes encoding BDNF and the proapoptotic Bcl 2 homolog Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SUBJECTS and METHODS : We performed immunohistochemical stains for bcl 2 , bax and in situ hybridization for EBV encoded RNA and the terminal deoxynucleotidyl transferase mediated dUTP digoxigenin nick end labeling technique using the 24 cases of GCLRS and 23 cases of gastric carcinoma with lymphoid poor stroma ( GCLPS ) out of 1 , 635 cases of gastric carcinoma . ^^^ Immunohistochemical expression for bcl 2 was revealed only in the cytoplasm of lymphocytes in all cases , but bax showed distinct expression in the cytoplasm of neoplastic cells in all cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Multidomain Bcl 2 homolog Bax but not Bak mediates synergistic induction of apoptosis by TRAIL and 5 FU through the mitochondrial apoptosis pathway . ^^^ These data clearly establish distinct roles for Bax and Bak in linking the TRAIL death receptor pathway to the mitochondrial apoptosis signaling cascade and delineate a higher degree of specificity in signaling for cell death by multidomain Bcl 2 homologs . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered expression of Bcl 2 , Bad and Bax mRNA occurs in the rat cerebellum within hours after ethanol exposure on postnatal day 4 but not on postnatal day 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Shh significantly increased the viability of CD34+ T cell precursors modulating bcl 2 and bax protein expression , and also inhibited their proliferation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , GFP Bax over expression system showed that Bax , pro apoptotic Bcl 2 family protein , was translocated to mitochondria by the presence of GJBRH . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Compared with SO , PO diabetic hearts demonstrated significantly higher levels of apoptosis , with an altered Bax : Bcl 2 ratio , augmented lipid peroxidation , and protein modification by formation of nitrotyrosine . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Combined treatment with low concentrations of these two agents also acted synergistically to induce apoptosis in HepG 2 cells through induction of Bax and Apaf 1 , reduction of Bcl 2 and Bcl xL , and activation of caspase 3 , 8 , and 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 / Bax mRNA expression ratio , used as an index to assess to what extent apoptosis contributed to tubular cell damage , and the expressions of HSP 90 , 72 and 27 in relation to the Bcl 2 / Bax ratio in the ischemic model , as parameters of their functional contributions to tubule cell damage , were also studied . ^^^ The Bcl 2 / Bax mRNA expression ratio increased progressively from the control to the heat preconditioned and ATP depleted cells ( control ; 100 % , ATP depletion ; 154 + / 6 % , heat preconditioning ; 212 + / 6 % , heat preconditioning and ATP depletion ; 421 + / 8 % ) . ^^^ CONCLUSION : There may be a possibility that the preservation of cytolytic damage and an increase in the Bcl 2 / Bax mRNA expression ratio is related to the increase of HSP 72 in ATP depletion as a hypoxia model . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In line with these results , 17 beta estradiol significantly increased Akt and cyclic AMP response element binding protein ( CREB ) with increased Bcl 2 protein in the ischemic area , whereas the elevated the phosphatase and tensin homolog deleted from chromosome 10 ( PTEN ) phosphorylation was significantly reduced with decreased Bax protein and cytochrome c release . ^^^ Taken together , it is suggested that suppression of cerebral ischemic injury by 17 beta estradiol may be ascribed to the maxi K channel opening coupled downregulation of PTEN phosphorylation and upregulation of Akt and CREB phosphorylation with resultant increase in Bcl 2 protein and decrease in Bax protein and cytochrome c release . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the balance between pro apoptotic ( i . e . , Bax ) and anti apoptotic ( i . e . , Bcl 2 or Bcl xL ) proteins , which was rather pro apoptotic after IR exposure , became anti apoptotic 24 h later , suggesting a protective effect . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fourteen days of unloading increased mRNA and protein levels of Bcl 2 , decreased protein levels of Bax , and decreased nuclear apoptosis inducing factor ( AIF ) protein level in muscles of aged birds . ^^^ There were significant correlations among levels of Bcl 2 , Bax , and AIF and TUNEL index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Detection of bcl 2 and bax expression and bcl 2 / JH fusion gene in intrahepatic cholangiocarcinoma . ^^^ AIM : To investigate the relationship between bcl 2 gene and its related protein bax and intrahepatic cholangiocellular carcinoma ( CCC ) . ^^^ METHODS : Semi nested in situ PCR ( SNISPCR ) and immunohistochemistry were performed to detect bcl 2 / JH fusion gene and bcl 2 , bax protein expression in 29 cases of CCC . ^^^ The ratio of bcl 2 to bax in normal liver tissues ( 3 . 5 to 1 ) was different from that in tumor tissues ( 7 . 0 to 1 ) . ^^^ However , aberrant ratio of bcl 2 to bax protein expression may be involved in the course of tumorigenesis of CCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , we found that the TG 2 BH3 peptides as well as TG 2 itself were able to interact with the pro apoptotic Bcl 2 family member Bax , but not with anti apoptotic members Bcl 2 and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax exert opposing effects on Ca2+ signaling , which do not depend on their putative pore forming region . ^^^ We expressed in HeLa cells Bcl 2 , Bax , and Bcl 2 / Bax chimeras in which the putative pore forming domains of the two proteins ( alpha 5 alpha 6 ) were mutually swapped , comparing the effects on Ca ( 2+ ) signaling of the two proteins and relating them to defined molecular domains . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Combination treatment induced an increase in intracellular reactive oxygen species ( ROS ) level , mitochondrial relocalization of B cell lymphoma 2 ( Bcl 2 ) associated 10 protein ( Bax ) , poly adenosine diphosphate ( ADP ) ribose polymerase 1 ( PARP 1 ) activation , and nuclear translocation of apoptosis inducing factor ( AIF ) . siRNA targeting of AIF effectively protected cells from the combination treatment induced cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also analyzed caspase 3 , 7 , 8 , 9 , Bcl 2 , Bax , Bid , and cytochrome C by Western analysis , and mitochondrial permeability and reactive oxygen species production by flow cytometry using mitosensor ( TM ) and DCFH DA , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Genistein increased expression of Bax and p21WAF1 and slightly decreased Bcl 2 level . ^^^ Our results demonstrated that genistein inhibited the viability of human colon cancer HT 29 cell via induction of apoptosis mainly through regulation of p21WAF1 and Bax / Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , a marked down regulation of the expression of the Bcl 2 , Bcl XL and XIAP , and up regulation of the Bax and Bad proteins were noted . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Staurosporine mediated activation of the pro apoptotic BCL 2 family member , BAX , was inhibited in the epithelial cell line infected for 32 h with the lymphogranuloma venereum ( LGV / L2 ) but not the murine pneumonitis ( MoPn ) strain of C . trachomatis , but inhibition of staurosporine mediated BAX activation disappeared after 48 h of infection with the LGV / L2 strain . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results showed that the expression levels of a pro apoptotic Bax protein , was significantly enhanced at the age of 24 months , while an anti apoptotic protein , Bcl 2 , was reduced in the aged rat kidney . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we discuss recent studies demonstrating that the death of antigen activated T lymphocytes during termination of an immune response is initiated by the BH 3 only Bcl 2 family member Bim and also requires its multi BH domain pro apoptotic relatives Bax and Bak . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 expression was without change in hepatocarcinoma cells treated with MF 13 ; however , a significant increase of bax was observed , resulting in a decreased ratio of bcl 2 / bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative RT PCR was employed to verify the profiling findings , and also used to examine a number of drug resistance related candidate genes ( MDR 1 , MRP 1 , MGMT , PTEN , BCL 2 , BAX , TP 53 and P 21 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A possible mechanism responsible for the different sensitivity in these two cell lines was explored by the examination of Bcl 2 , Bax , Bak , and Fas . ^^^ The cell death stimulus increased anti apoptotic Bcl 2 and decreased pro apoptotic Bcl 2 members ( Bak and Bax ) and Fas in glioblastoma cells deficient in DNA PK . ^^^ Activation of DNA PK is known to promote cell death of human tumor cells via modulation of p 53 , which can down regulate the anti apoptotic Bcl 2 member proteins , induce pro apoptotic Bcl 2 family members and promote a Bax Bak interaction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Elevated expression of Bax , one of the pivotal proapoptotic proteins of the Bcl 2 family , has been found in human atherosclerotic plaques . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic cells , Bcl 2 and Bax positive cells were located and calculated by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling ( TUNEL ) , immunohistochemical method and retinal whole mount combined with section technique at day 4 , 7 , 14 after crush , respectively . ^^^ RESULTS : The TUNEL labeling cells , Bcl 2 and Bax positive cells were detected in the ganglion cell layer of retina . ^^^ Few apoptotic cells , a few of Bcl 2 and Bax positive cells were observed in normal control groups . ^^^ The number of apoptotic cells and Bax positive tells in treatment groups was less than that in crush group significantly ( P < 0 . 05 ) , while the number of Bcl 2 positive cells in treatment group was more than that in crush group at day 4 , 7 and 14 after crush significantly ( P < 0 . 05 ) . ^^^ CONCLUSIONS : These results suggest that high dose MP can inhibit the apoptosis of RGC after optic nerve crush probably through up regulation of Bcl 2 expression and down regulation of Bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Biopsies of mammary gland tissue were collected at 50 days after lambing for in situ detection of cell death and RT PCR analysis of bax , bcl 2 , caspase 3 and GST expressions . ^^^ There was a significant increase in the ratio of bcl 2 to bax mRNA in the HS and HF groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protective effect of expressing Slug can be reversed by expressing the apoptotic factor Bax , while the apoptosis promoted by msx 1 can be abolished by expressing the Xenopus homologue of Bcl 2 ( XR 11 ) . ^^^ In addition , expression of Bax or Bcl 2 , produced similar effects on the survival of the neural crest and on the development of its derivatives to those produced by altering the activity of Slug or msx 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The goals of the present study were : 1 ) to document the time course and amount of cell death ( both apoptotic and necrotic ) , and 2 ) to investigate the effect of GABA ( A ) receptor activation on the time course and expression of three cell death related proteins ( caspase 9 , bax and bcl 2 ) in our model of prenatal brain injury . ^^^ There was a relative increase in the amount of bax to bcl 2 protein , and increased protein levels of caspase 9 , indicative of cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expressions of Bax , Bak , and Bcl 2 mRNA in the pancreas were detected by reverse transcriptase polymerase chain reaction ( RT PCR ) on day 3 and at weeks 1 , 2 , 3 , and 4 . ^^^ Meanwhile , Bax and Bak mRNA expression was increased , but Bcl 2 mRNA expression was decreased , as compared with the normal group . ^^^ The administration of losartan resulted in inhibition of acinar cell apoptosis and down regulation of Bax , Bak , and Bcl 2 mRNA expression . ^^^ The Bax / Bcl 2 ratio was lower in losartan treated rats than in control rats . ^^^ This action may be associated with its regulation of apoptosis associated genes , such as Bax , Bak , and Bcl 2 mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 , Bcl xl , Bak and Bax proteins in axons of the optic nerve in closed angle glaucoma ] . ^^^ PURPOSE : The aim of our study was the evaluation of Bcl 2 , Bcl xl , Bak and Bax immunoexpression in axons of the optic nerve with absolute glaucoma . ^^^ The samples were immuno stained with antibodies for Bcl 2 , Bak , Bax and Bcl xl protein . ^^^ RESULTS : In our study proapoptotic Bak and Bax protein expression was stronger than antiapoptotic Bcl 2 , Bcl xl protein expression ; Bak / Bcl 2 ( p = 0 . 008 ) , Bax / Bcl 2 ( p = 0 . 0007 ) , Bak / Bcl xl ( p = 0 . 0356 ) , Bax / Bcl xl ( p = 0 . 0077 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting analyses revealed that IFN gamma dramatically increased the protein levels of interferon regulatory factor ( IRF ) 1 , but not TRAIL receptors ( DR 4 and DR 5 ) and pro apoptotic ( FADD and Bax ) and anti apoptotic factors ( Bcl 2 , Bcl XL , cIAP 1 , cIAP 2 and XIAP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our data suggest that the inhibitory effect of phthalates on TAM induced apoptosis involves an increase in intracellular Bcl 2 to Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While SCF up regulated the expression of the anti apoptotic proteins Bcl 2 and Bcl xL , it did the opposite to the pro apoptotic factor Bax . ^^^ The PI3K inhibitor reversed the regulation of SCF on Bcl xL and Bax but not on Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The combination treatment activated caspase 3 and cleaved PARP , but it did not induce any notable change in the expression of Bcl XL , Bcl 2 and Bax compared with each single treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since the cellular mechanisms mediating this effect are not well defined , we investigated the activity of LA , also in combination with DHT or with cyproterone acetate ( CA ) , on the expression of genes ( bcl 2 , bax , c myc ) which may contribute to the proliferative behaviour of LNCaP cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The growth inhibitory effects of resveratrol are mediated through cell cycle arrest ; upregulation of p21Cip1 / WAF1 , p 53 and Bax ; down regulation of survivin , cyclin D 1 , cyclin E , Bcl 2 , Bcl xL and clAPs ; and activation of caspases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The combined immunohistochemical expression levels of the proteins bax , bak , bad , bid , bcl 2 and bcl xl were evaluated by cluster and discriminant analysis . ^^^ RESULTS : Cluster analysis produced : a ) a low expression ( 69 / 79 cases ) and a high expression pro apoptotic cluster ( 10 / 79 cases ) for the combined expression levels of the pro apoptotic proteins bax , bak , bad and bid and b ) a low expression ( 37 / 76 cases ) and a high expression antiapoptotic cluster ( 39 / 76 cases ) for the combined expression levels of anti apoptotic proteins bcl 2 and bcl xl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present paper , we demonstrate that the absence of apoptosis in HIV 1 infected primary human monocyte differentiated macrophages ( MDM ) correlates with an increase in anti apoptotic ( Bcl 2 and Bcl 10 ( L ) ) and a decrease in pro apoptotic ( Bax and Bad ) proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Also , upregulation of pro apoptotic protein Bax , and downregulation of anti apoptotic protein Bcl 2 by presence of CoCl ( 2 ) appeared significantly and it might result in activating mitochondria mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Influence of lovastatin on BCL 2 and BAX expression by plasma cells and T lymphocytes in short term cultures of multiple myeloma bone marrow mononuclear cells . ^^^ In this study , we analyzed BCL 2 and BAX expression in cells of MM patients exposed to LOV in short term culture . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of genes influencing apoptosis , such as bcl 2 , p 53 , and bax , may rather be of importance from the point of view of histological differential diagnosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell apoptosis , and expression changes of Bcl 2 and Bax were observed by FCM , reverse transcriptase polymerase chain reaction ( RT PCR ) , and Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We demonstrated that UA induced apoptosis was dependent on the mitochondrial intrinsic pathway , as shown by transmembrane potential collapse ( DeltaPsim ) and by alteration of the Bax Bcl 2 balance , with a concomitant increase in Bax expression and decrease in Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting was used to observe the expression of p 53 , p 21 ( WAF 1 ) , Bax , and Bcl 2 protein . ^^^ The expression levels of protein p 53 and Bcl 2 decreased following 2 methoxyestradiol treatment in CNE 2 cells , whereas Bax and p 21 ( WAF 1 ) protein expression were unaffected after treatment with 2 methoxyestradiol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By using rats exposed in utero to the anti androgenic compound flutamide ( 0 . 4 , 2 or 10 mg / kg per day ) , it has been shown that hypospermatogenesis in adult testes could be related to ( 1 ) a long term apoptosis in germ cells but not in somatic Leydig and Sertoli cells as evidenced by the TUNEL approach and ( 2 ) alterations in the mRNA and protein expression of pro ( Bax , Bak , Bid ) and anti apoptotic ( Bcl 2 , Bcl w ) members of the Bcl 2 family . ^^^ Moreover , for the Bcl 2 related proteins that were expressed mainly in the germ cells , a decrease in the levels of anti apoptotic peptides Bcl w ( 60 % , P=0 . 003 ) and Bcl 2 ( 90 % , P=0 . 0001 ) was observed at 2 mg / kg per day flutamide and an increase in levels of the pro apoptotic Bax ( 2 . 3 fold , P=0 . 0004 ) was detected at 10 mg / kg per day . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 was significantly increased in MXC and HPTE treated cells , while bax was decreased in MXC and HPTE treated cells compared to controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we show that GSK 3beta phosphorylates and regulates the activity of Bax , a pro apoptotic Bcl 2 family member that stimulates the intrinsic ( mitochondrial ) death pathway by eliciting cytochrome c release from mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Anti apoptotic Bcl 2 mRNA levels were markedly reduced and pro apoptotic Bax mRNA levels were markedly elevated in the NEC group compared with DF controls . ^^^ Supplementation of EGF into formula significantly increased anti apoptotic Bcl 2 mRNA , whereas pro apoptotic Bax was significantly decreased . ^^^ The Bax to Bcl 2 ratio for mRNA and protein was markedly decreased in NEC+EGF animals compared with the NEC group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family proapoptotic protein , Bax , redistributes to the mitochondrion in response to varied stimuli , triggering loss of mitochondrial integrity and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DNA fragmentation and the apoptosis associated proteins Bcl 2 , Bax , Bim , P 53 as well as caspases 8 and 3 were used as markers of apoptosis . ^^^ RESULTS : Topical application of DMBA for 14 weeks resulted in well developed squamous cell carcinomas ( SCCs ) associated with increased expression of Bcl 2 and decreased expression of Bax , Bim , P 53 and caspases 8 and 3 . ^^^ This was evidenced by downregulation of Bcl 2 and upregulation of Bax , Bim , P 53 and caspases 8 and 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Since ischemia can be related to not only necrosis but apoptosis as well , we compared the development of apoptosis in STZ diabetic rats and STZ diabetic rats subjected to occlusion of the middle cerebral artery ( MCA ) . 24 48 hr following MCA occlusion the animals were killed , the brain removed and prepared for evaluation by several indexes of apoptosis : nucleosomal DNA fragmentation , TUNEL staining , activation of caspase 3 and alteration in the expression of Bax and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Whereas Bax or Bak were shown to be required for the proapoptotic effect of Bid , Bcl 2 was described to interfere with its action . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase was accompanied by a marked inhibition of Bcl 2 expression and an increase in BAX expression , a pattern of gene expression suggestive of a pro apoptotic shift . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Renal cell carcinoma MIB 1 , Bax and Bcl 2 expression and prognosis . ^^^ In this family the Bcl 2 gene has an inhibitory effect on apoptosis , while Bax promotes cell death . ^^^ We evaluated Bax and Bcl 2 levels in RCC , and their associations with prognosis , proliferation and traditional prognostic factors . ^^^ Immunostaining and semiquantitative indices for Ki 67 ( MIB 1 ) , Bax and Bcl 2 were estimated . ^^^ RESULTS : On univariate analysis according to survival statistically significant differences were achieved by Bax ( positive vs negative HR 3 . 04 , 95 % CI 1 . 27 to 7 . 23 ) , Bcl 2 ( positive vs negative HR 0 . 43 , 95 % CI 0 . 23 to 0 . 81 ) , MIB 1 ( continuous HR 1 . 03 , 95 % CI 1 . 001 to 1 . 064 ) , Fuhrman nuclear class ( 4 vs 1 plus 2 HR 8 . 15 , 95 % CI 3 . 13 to 21 . 20 ) and stage ( 4 vs 1 HR 60 . 04 , 95 % CI 13 . 99 to 257 . 68 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
With regard to the effects of QdNOs on molecules that regulate apoptosis and the G 2 to M transition , both BPQ and AMQ inhibited the expression of cyclin B , while DCQ significantly decreased the levels of Bcl 2 and increased Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses were performed on cultured seminiferous tubule segments for Bcl 2 family proteins ( Bax , Bad , Bcl w , Bcl xL ) and fas ligand . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No effect of the two cytokines on major members of apoptosis regulating systems ( CD 95 , CD95L , bcl 2 , bax , bcl xL , p 53 , p21WAF1 , p 27 , NFkappaB ) could be observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , Bax , FLIP and p 53 , activation of caspases 3 , 8 and 9 , and cleavage of poly ( adenosine diphosphate ribose ) polymerase ( PARP ) in the epidermis were analysed by Western blotting in four patients with each type . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bcl XL inhibit apoptosis by binding to proapoptotic proteins such as Bax , thereby preventing chemotherapy induced or radiation induced release of cytochrome c from mitochondria and subsequent activation of the caspase protease cascade . ^^^ In this report , we show that cell permeable Bax BH 3 peptides physically disrupt Bax / Bcl 2 heterodimerization in intact cells and that this disruption correlates with peptide induced cell death . ^^^ A point mutant , control peptide that failed to disrupt intracellular Bax / Bcl 2 interactions also failed to promote apoptosis . ^^^ Peptides were quantitatively examined for their ability to inhibit Bax / Bcl 2 and Bax / Bcl XL heterodimerization in vitro and to promote cytochrome c release from mitochondria isolated from Jurkat , HL 60 , U 937 , and PC 3 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced by BaP 1 on endothelial cells was independent of two Bcl 2 family members ( anti apototic Bcl xL and pro apoptotic Bax ) , that did not show any changes in their expression during a 24 h treatment period . ^^^ In conclusion , treatment of human endothelial cells with BaP 1 induces apoptosis / anoikis , independently of Bcl 2 family members Bax and Bcl xL and associated with caspase 8 activation and cFLIP ( L ) up regulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HMBA , used at cytostatic concentrations , reduced the ratio be tween antiapoptotic ( Bcl 2 , Bcl XL ) and proapoptotic ( Bax ) members of the Bcl 2 family of proteins , thus lowering the threshold for MM cell death commitment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using lysate RNase protection assays , mRNA expression of the anti cell death genes Bcl 2 and Bcl xL , and the pro cell death gene Bax , was evaluated following experimental brain injuries in adult male Sprague Dawley rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results demonstrated that 16 genes ; bad , bax , bcl 2 , bcl w , bcl 10 , caspase 3 , caspase 7 , caspase 8 , c myc , E 124 , GADD 45 , mdm 2 , NKkappab 1 , p 53 , p 21 , Rb and trail were up regulated and six genes ; caspase 1 , caspase 2 , DR 5 , E2F1 , FasL and iNOS did not changed in response to DES treatment in wild type mice compared to p53+ / knockout mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In study 2 , mouse bone marrow from B6D2F1 mice was sampled every 3 h for 24 h in order to confirm the BCL 2 rhythm and to study its relation with 24 h changes in the expression of proapoptotic BCL 2 associated 10 protein ( BAX ) protein and clock genes mPer 2 , mBmal 1 , mClock , and mTim mRNAs . ^^^ Conversely , a molecular circadian clock likely regulated BCL 2 and BAX expression in the bone marrow , increasing cellular protection against apoptosis during the rest span . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in apoptosis was associated with a dose dependent up regulation in proapoptotic Bax expression and down regulation of anti apoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , bax mRNA level was up regulated whereas a decline in Bcl 2 both protein and mRNA levels were detected in NaBu treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In other studies , IP 6 caused a dose and a time dependent apoptotic death of LNCaP cells , and a decrease in Bcl 2 levels , causing a strong increase in Bax versus Bcl 2 ratio , as well as an inhibition of constitutively active AKT phosphorylation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of Bcl 2 or Bcl 10 ( L ) , loss of Bax or Bak function , high expression of inhibitor of apoptosis proteins , and reduced release of second mitochondria derived activator of caspases ( Smac / Diablo ) from the mitochondria to the cytosol have all been reported to result in TRAIL resistance in mitochondria dependent type 2 cancer cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in expression of bcl 2 and bax in Syrian hamster embryo ( SHE ) cells exposed to ZnCl 2 . ^^^ Up regulation of bcl 2 expression was found and bax expression was reduced . ^^^ Effects of Zn ( 2+ ) on bcl 2 / bax ratio were confirmed in apoptotic camptothecin treated SHE cells . ^^^ This study demonstrated that zinc acts in the early phases of the apoptotic process by modification of the bcl 2 / bax ratio in normal and apoptotic SHE cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis state of cells was observed by morphological examination , flow cytometry and TUNEL method , and the cell apoptosis related gene expression of bcl 2 and bax were determined by S P immunocytochemical assay . ^^^ Comparison between the GFP treated group and the blank control group on intracellular bcl 2 gene expression showed no difference , but bax gene expression was higher in the GFP treated group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of antidigoxin antiserum on endoxin levels , apoptosis and the expression of Bax and Bcl 2 protein in ischaemia reperfusion myocardium . ^^^ The aim of the present study was to investigate the effects of antidigoxin antiserum ( ADA ) , an endoxin special antagonist , on endoxin levels , apoptosis and the expression of the apoptosis related protein bcl 2 and bax in myocardial ischaemia reperfusion ( MIR ) . ^^^ The expression of the apoptosis related proteins bcl 2 and bax was detected by immunohistochemistry and their semiquantification scores were recorded by a computer image analysis system . ^^^ However , the ratio of bcl 2 / bax was significantly decreased in the MIR group . ^^^ In the group receiving 36 mg / kg ADA , myocardial endoxin levels , the number of apoptotic cells and bax protein expression were significantly decreased ; bcl 2 protein expression was enhanced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In wild type mice , TNBS treatment resulted in colonic ulceration and marked apoptosis , which was associated with decreased colon content of the antiapoptotic protein Bcl 2 , whereas the proapoptotic Bax was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Relationship between apoptosis of mouse thymic lymphocytes and expressions of bax , bcl 2 and bcl XL after gamma ray radiation with lethal dose ] . ^^^ AIM : To observe the relationship between apoptosis of mouse thymic lymphocytes and the expressions of bax , bcl 2 and bcl 10 ( L ) after gamma ray radiation with lethal dose and provide the basis for treatment of acute severe radiation sickness . ^^^ The expressions of bax , bcl 2 and bcl 10 ( L ) were detected by in situ hybridization and immunohistochemical staining . ^^^ Analysis of bax and bcl 2 mRNAs was concordant with the protein expression results . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , pretreatment of LLC cells with ceramide / DMS not only increased the protein expression level of Bax , but also decreased Bcl 2 after gamma irradiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bcl 2 family of proteins are well characterized regulators of apoptosis , and the multidomain pro apoptotic members of this family , such as Bax and Bak , act as a mitochondrial gateway where a variety of apoptotic signals converge . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show an increased expression of Bcl 2 and heat shock protein 27 and a downregulation of Bax in the PDT resistant variants . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In vivo apoptosis correlated with significant decrease in activation of protein kinase B / Akt , a cell survival signaling kinase , with increased expression of the proapoptotic protein Bax and decreased expression of the antiapoptotic protein Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 , as well as the proapoptotic protein Bax , is regulated by p 53 . p 53 also induces MDM 2 transcription , which functions to limit further p 53 induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MUC 4 is increased in high grade intraepithelial neoplasia in Barrett ' s oesophagus and is associated with a proapoptotic Bax to Bcl 2 ratio . ^^^ AIMS : To determine the expression pattern of mucins in neoplastic BO epithelium ( high grade dysplasia ) and correlate it with the expression of apoptosis markers Bax and Bcl 2 . ^^^ MUC 1 4 , MUC5AC , MUC5B , MUC 6 , Bax , and Bcl 2 mRNA were determined by semiquantitative RT PCR . ^^^ The Bax : Bcl 2 ratio was increased in HGN compared with BO ( p = 0 . 04 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bcl 2 family members , Bcl xL , Bcl 2 , Mcl 1 and Bax was investigated in delayed apoptosis of canine neutrophils induced by lipopolysaccharide ( LPS ) . ^^^ The levels of Bcl xL , Bcl 2 , Mcl 1 and Bax transcripts at 9 h incubation in neutrophils stimulated by LPS ( 100 ng / ml ) were increased by about 80 . 4 , 1 . 9 , 1 . 4 and 5 . 3 folds , in comparison to those in non stimulated neutrophils , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We conclude that Ant inhibits apoptosis in preovulatory follicles through a decrease of BAX translocation to mitochondria , suggesting that GnRH may act as a physiological intraovarian modulator factor that is able to interfere with follicular development through an increase in apoptotic events mediated by an imbalance among the BCL 2 family members . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a pro apoptotic member of the Bcl 2 family of proteins involved in the regulation of genetically programmed cell death in mammalian cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cinnamaldehyde also upregulated the expression of pro apoptotic protein ( Bax ) and down regulated the levels of anti apoptotic proteins , such as Bcl 2 and the inhibitor of apoptosis protein family ( 10 linked inhibitor of apoptosis protein ( XIAP ) , cellular inhibitor of apoptosis protein ( cIAP ) 1 and cIAP 2 ) . 4 . ^^^ The expression of apoptotic inhibitors ( XIAP , cIAP 1 , cIAP 2 ) and anti apoptotic ( Bcl 2 ) and pro apoptotic ( Bax ) proteins was affected by vitamin E pretreatment . 5 . ^^^ Pretreatment with vitamin E markedly prevented cinnamaldehyde mediated apoptosis , which was associated with the modulation of XIAP , cIAP 1 , cIAP 2 , Bcl 2 and Bax protein activity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OGD R caused apoptotic cell death , induced cytochrome C release from mitochondria and caspase 3 activation , decreased mitochondrial membrane potential , and increased levels of pro apoptotic Bax translocated to the mitochondrial membrane , all of which were reversed by overexpression of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We were interested in the relationship of the after doxorubicin treatment AML cells apoptosis and the immunophenotype , selected clinical and laboratory parameters , and also the P gp , MRP , LRP , Bcl 2 , Bax proteins expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
C 2 ceramide stimulated Bax expression , but had no effect on Bcl 2 , while PACAP abrogated the action of C 2 ceramide on Bax and stimulated Bcl 2 expression . ^^^ The effects of C 2 ceramide and PACAP on Bax and Bcl 2 were blocked , respectively , by the JNK inhibitor L JNKI 1 and the MEK inhibitor U 0126 . ^^^ The present study reveals that C 2 ceramide and PACAP exert opposite effects on Bax and Bcl 2 through , respectively , JNK and ERK dependent mechanisms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We analyzed the methylation status of the 5 ' regions of 12 apoptosis associated genes ( ARF , FADD , TNFRSF 21 , BAX , LITAF , DAPK , TMS 1 , BCL 2 , RASSF1A , TERT , TNFRSF 25 , and EDNRB ) in 18 bladder cancer cell lines , 127 bladder cancer samples , and 37 samples of adjacent normal bladder mucosa using the quantitative MethyLight assay . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have evaluated by multiparameter flow cytometry the expression of apoptosis ( APO2 . 7 , bcl 2 , and bax ) and MDR related proteins [ P glycoprotein ( P gp ) , multidrug resistance protein ( MRP ) , and lung resistance protein ( LRP ) ] specifically on bone marrow ( BM ) CD34+ cells , and their major CD 32 / dim and CD32+ subsets , in de novo AML ( n=90 ) , high risk myelodysplastic syndrome ( n=9 ) , and low risk myelodysplastic syndrome ( n=21 ) patients at diagnosis , and compared with normal BM CD34+ cells ( n=6 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , we demonstrate that Daxx overexpressing myelocytes downregulate the proapoptotic Bcl 2 family member Bax , while expression of antiapoptotic Bcl 2 is not influenced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In human neuroblastoma cell lines ( LAN 5 , SHEP and IMR 32 ) , mycophenolic acid ( MPA ) at concentrations ( 10 ( 7 ) 10 ( 6 ) M ) readily attainable during immunosuppressive therapy with mycophenolate mofetil ( Cellcept ) , induces guanine nucleotide depletion leading to cell cycle arrest and apoptosis through a p 53 mediated pathway ( up regulation of p 53 , p 21 and bax and down regulation of bcl 2 and survivin ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results also showed a decrease in mitochondrial Bax and an increase in mitochondrial Bcl 2 levels upon exposure to FK 506 and 3 NP . ^^^ However , no significant changes occurred in total Bcl 2 and Bax levels . ^^^ Altogether , the results suggest that FK 506 neuroprotection against 3 NP induced apoptosis is associated with the redistribution of Bcl 2 and Bax in the mitochondrial membrane . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
By contrast , type 2 signaling was facilitated by p38alpha dependent mitochondrial presence of tBid and inhibition of Bcl 2 ( Ser 70 ) phosphorylation as well as by p38alpha / beta dependent mitochondrial localization of Bax and inhibition of phosphorylation of Bad ( Ser112 / Ser155 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Their neuroprotective effect has been demonstrated to be associated directly with the propargylamine moiety , which protects mitochondrial viability and MTPp by activating Bcl 2 and protein kinase C ( PKC ) and by downregulating the proapoptotic FAS and Bax protein families . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism by which some BH 3 only proteins of the Bcl 2 family directly activate the `` multidomain ' ' proapoptotic member Bax is poorly characterized . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These effects are inhibited by activated Akt , but not by Bcl 2 , implying that changes in outer mitochondrial membrane ( OMM ) permeability leading to apoptosis can occur in the absence of Bax and Bak and that Akt inhibits these changes through maintenance of hexokinase association with mitochondria . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic proteins K 7 and K 15 from avian encephalomyelitis virus ( AEV ) and viral mitochondria inhibitor of apoptosis ( vMIA ) from cytomegalovirus are capable of binding host specific apoptosis modulatory proteins such as Bax , Bcl 2 , activated caspase 3 , CAML , CIDE B and HAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL 2 , BAX , FAS , caspase 9 , caspase 3 and BCL2L12 were amplified by PCR . ^^^ In the case of carboplatin induced apoptosis we detected down regulation of BAX , BCL 2 and caspase 9 , whereas in the case of doxorubicin , BAX and BCL 2 remained at control levels and caspase 9 was increased . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine the significance of apoptosis in CMD1A pathogenesis , we determined whether pathogenesis in laminin alpha 2 deficient ( Lama 2 ( / ) ) mice could be ameliorated by inhibiting apoptosis through either ( a ) inactivation of the proapoptosis protein Bax or ( b ) overexpression of the antiapoptosis protein Bcl 2 from a muscle specific transgene . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An up regulated expression of Bcl 2 and Bcl 10 ( L ) proteins was observed in ECSC in comparison with ESCwE and NESC , whereas the levels of Bax , Bad , Fas and Fas ligand proteins in ECSC were similar to those in ESCwE and NESC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This neuroprotection is accompanied by an inhibition of NMDA receptor mediated calcium influx , upregulation of anti apoptotic Bcl 2 , downregulation of pro apoptotic p 53 and Bax , and activation of cell survival factors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism of RGC death was investigated using TUNEL staining , immunostaining for cleaved caspase 3 , and Western blot for Bcl 2 and Bax expression . ^^^ RT PCR was used to measure changes in Bcl 2 , Bax , Bad , Bak , P 53 , ICE and Fas . ^^^ Bcl 2 expression in control group was higher than in the experimental group , while Bax expression in the control group was less than in experimental group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that combinations of ICI 182780 and BE 3 3 3 caused down regulation of the anti apoptotic Bcl 2 and Bcl XL proteins and increased the level of the pro apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All patients were treated by BOAI , and expression of cancer cell apoptosis was examined by the TUNEL method , expression of bax , bcl 2 and bcl xL proteins were examined by immunohistochemistry , and expression of bax , bcl 2 and bcl xL mRNA was examined by quantitative RT PCR before and 3 days after BOAI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 protein was similar in all three groups , making the ratio of Bax / Bcl 2 significantly higher in the hypercapnic groups than in the normocapnic group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis regulators p 53 , bcl 2 and bax , and the growth factor receptors EGFR and HER 2 / neu were analyzed by immunohistochemical techniques and DNA analysis was performed by flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of anti apoptotic Bcl 2 and Mcl 1 / EAT was up regulated 4 h after the treatment , while pro apoptotic Bax expression did not change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis measured the expression of bcl 2 and bax proteins . ^^^ Western blot analysis revealed downregulation of bcl 2 and upregulation of bax proteins following treatment with oridonin for 48 h . ^^^ We conclude that oridonin demonstrated anti proliferative and apoptosis inducing effects on SPC A 1 cells in vitro , and that changes in bcl 2 and bax protein levels may play an important role in its mechanism of action . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Dexamethasone protected human glioblastoma U87MG cells from temozolomide induced apoptosis by maintaining Bax : Bcl 2 ratio and preventing proteolytic activities . ^^^ Western blot analyses showed alternations in the levels of Bax ( pro apoptotic ) and Bcl 2 ( anti apoptotic ) proteins resulting in increased Bax : Bcl 2 ratio in TMZ treated cells . ^^^ However , 1 h pretreatment of cells with 40 microM DXM dramatically decreased TMZ induced apoptosis , decreasing Bax : Bcl 2 ratio and SBDPs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We analyzed cell proliferation ( immunohistochemistry of Ki 67 and spectrophotometric assay ) ; apoptosis ( DNA fragmentation ( TUNEL ) and Annexin 5 FITC binding ) ; ER alpha , ER beta bcl 2 and bax mRNA abundance ( RT PCR ) in explants and isolated endometrial epithelial ( EEC ) and stromal cells ( ESC ) incubated with androstenedione 1 micro mol / l ( A 4 ) or A 4 plus hydroxyflutamide 10 micro mol / l ( F ) for 24 h . ^^^ In isolated ESC , proliferation augmented with A 4 ( p < 0 . 05 ) , whereas , no significant modifications in the expression of ER alpha , ER beta bcl 2 and bax nor in the apoptotic index were observed . ^^^ In EEC , A 4 increase the ER beta mRNA abundance ( p < 0 . 05 ) and a decrease of the bcl 2 / bax ratio ( p < 0 . 05 ) , without an increase in the apoptotic index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we show that the molecular pathway leading to TFEC mediated cell death is associated with an early cytosolic to mitochondrial translocation of BAX , a pro apoptotic member of the BCL 2 family . ^^^ Hence , TFEC induced necrotic cell death in the TAMH cell line is mediated by BAX and antagonized by the anti apoptotic BCL 2 family member , BCL xL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We employed mouse bone marrow derived mast cells ( BMMC ) to measure the effects of IL 4 + IL 10 on survival and cell cycle progression . p 53 Deficient , bax deficient , and bcl 2 transgenic BMMC were compared to wild type cells to determine the role of these proteins in apoptosis . ^^^ Cell death was accompanied by loss of mitochondrial membrane potential , the importance of which was demonstrated by resistance to IL 4 + IL 10 mediated cell death when Bax was deleted or Bcl 2 was overexpressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although most studies focus on phosphorylation of pro and antiapoptotic proteins ( BAD , Bax , Bcl 2 , Bcl xL ) , kinase mediated regulation of complex 1 activity , anion and cation channels , metabolic enzymes , and Mn SOD mRNA has also been reported . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further understand the apoptotic molecular mechanism of lycorine on HL 60 cells , caspase activity was tested by colorimetric assay , and the expression of Bcl 2 and Bax proteins was examined by Western blotting . ^^^ Under expression of Bcl 2 and increase of Bax : Bcl 2 ratio showed that Bcl 2 family proteins were involved in apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition to the caspase 3 activation , capsaicin also induced cytochrome c release and decrease in Bcl 2 protein expression with no changes in the level of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Attenuation of ischemia and / or reperfusion injury during myocardial infarction using mild hypothermia in rats : an immunohistochemical study of Bcl 2 , Bax , Bak and TUNEL . ^^^ Immunohistochemical analysis was carried out using antibodies against Bcl 2 , Bax and Bak . ^^^ In the HT group Bcl 2 was induced in many myocytes , whereas Bax and Bak were induced in only a few myocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
STUDY DESIGN : Tissue sections were incubated with primary antibodies reactive to neuron specific enolase ( NSE ) , vimentin , desmin , S 100 protein , cytokeratins AE 1 AE3 , EMA , neurofilament , synaptophysin , and muscle specific actin ( MSA ) , and for prognostic markers , including Ki 67 , p 53 , bcl 2 , bax , and nm 23 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cardiolipin is important for the proapoptotic activity of Bcl 2 family members such as Bid , Bax and Bak , and modulation of its metabolism and translocation in mitochondrial membranes could potentially have a strong influence on apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Only a minor fraction of the overexpressed Bcl 2 gets phosphorylated in tamoxifen treated cells and the phosphorylation does not affect its binding to Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we examined the effects of GLN on GSH levels in DMBA induced mammary tumors and correlated the results with protein and mRNA expression of apoptosis related proteins Bcl 2 , Bax and caspase 3 in tumor cells . ^^^ The results have shown that GLN supplementation caused a significant decrease in the tumor GSH levels and the ratio GSH / oxidized GSH ( GSSG ) , accompanied by up regulation of Bax and caspase 3 , and down regulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EGCG treatment of melanoma cells resulted in a downmodulation of anti apoptotic protein Bcl 2 , upregulation of proapoptotic Bax and activation of caspases 3 , 7 and 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Paraffin embedded sections of 19 radicular cysts ( RCs ) , and five residual radicular cysts ( RRCs ) and control specimens of normal gingivae excised from seven cadavers were prepared and examined immunohistochemically with the use of monoclonal antibodies or polyclonal antisera against single stranded DNA ( ssDNA ) , p 53 , Bax , Bcl 2 , caspase 3 , Fas , Fas ligand ( Fas L ) , and Ki 67 antigen . ^^^ Bax and Bcl 2 were detected in some basal epithelial cells in RCs and RRCs as well as in gingiva . ^^^ The ssDNA reactivity significantly increased parallel to Bax expression and slightly decreased parallel to Bcl 2 expression in lining epithelium of RCs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic BCL 2 family members BAX and BAK are required for the initiation of mitochondrial dysfunction during apoptosis and for maintaining the endoplasmic reticulum ( ER ) Ca ( 2+ ) stores necessary for Ca ( 2+ ) dependent cell death . ^^^ BCL 2 and IP3R 1 physically interact at the ER , and their binding is increased in the absence of BAX and BAK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that pretreatment with AP could block the upregulation of pro apoptotic p 53 , Bax , and Bad and the downregulation of Bcl 2 by irradiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After 42 days , creatinine clearance ; blood CsA level ; apoptosis ; inflammation and tubulointerstitial fibrosis in renal tissue ; and the expression of Bax , Bcl 2 , Fas , FasL , and transforming growth factor ( TGF ) beta protein were determined . ^^^ RESULTS : CsA induced tubular cell apoptosis ; cellular infiltration ; and increase of Fas , Bax , TGF beta protein expression with significant tubulointerstitial fibrosis , and reduced Bcl 2 protein expression . alpha MSH treatment prevented the Bax and TGF beta protein increase and induced Bcl 2 protein increase , together with reduction of apoptosis , inflammation , and tubulointerstitial fibrosis . ^^^ CONCLUSIONS : These findings suggest that chronic CsA nephrotoxicity is related to Bax and Bcl 2 related apoptosis pathways , and that alpha MSH can attenuate the CsA induced tubulointerstitial fibrosis as well as tubular cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism of neuroprotective activity has been attributed to the ability of propargylamines inducing the antiapoptotic family proteins Bcl 2 and Bcl xl , while decreasing Bad and Bax and preventing opening of mitochondrial permeability transition pore . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To measure the expression of inducible nitric oxide synthase ( iNOS ) , Bcl 2 , Bax protein and expression of Bcl 2 , Bax gene , immunohistochemistry and in situ hybridization have been explored respectively . ^^^ Furthermore , Re could enhance the expression of Bcl 2 protein and Bcl 2 mRNA , but reduce the expression of Bax , Bax mRNA , and iNOS , and weaken the cleavage of caspase 3 . ^^^ In summary , ginsenoside Re showed protection from MPTP induced apoptosis in the PD model mouse nigral neurons and this effect may be attributable to upregulating the expression of Bcl 2 protein , downregulating the expression of Bax , and iNOS protein , and inhibiting the activation of caspase 3 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ALT combined with PNA or RTX exerted significantly higher proapoptotic effect in vitro than single agents , downregulating FLIP and Bcl 2 ( ALT + PNA ) or significantly increasing Bax expression ( ALT + RTX ; P = 0 . 007 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Peripheral blood B cells were isolated and stained for apoptosis markers ( Bax , Bcl 2 ) and members of the TNF R superfamily , CD 95 and CD 40 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also measured serum levels of oxidative stress markers [ malondialdehyde ( MDA ) and homocysteine ] , lycopene , vitamins C , E , and A , and tissue biomarkers Bcl 2 and Bax expression . ^^^ There were no significant differences in the expression of tissue Bcl 2 and Bax among the study groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An analysis of intrinsic arm of apoptotic signaling cascade elements revealed that PGF ( 2alpha ) injection triggered increased ratio of Bax to Bcl 2 in the luteal tissue as early as 4 h posttreatment that remained elevated until 18 h . ^^^ This increase was associated with the elevation in the active caspase 9 and 3 protein levels and activity ( p < 0 . 05 ) at 4 12 h , but a spurt in the activity was seen only at 18 h posttreatment that could not be accounted for by the changes in the Bax / Bcl 2 ratio or changes in translocation of Bax to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 inhibited TRAIL induced Bax translocation , cytosolic release of cytochrome c and Smac / DIABLO , and the downstream cleavage of XIAP and DFF 45 . ^^^ Coadministration of HA 14 1 and TRAIL increased apoptosis in SW480 / Bcl 2 cells by restoring Bax redistribution and cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In vivo , these cells have an antiapoptotic phenotype ( high levels of Bcl 2 and low levels of proapoptotic Bcl 2 family proteins , such as Bax ) . ^^^ METHODS : To investigate apoptosis regulation , Bcl 2 , Bax , mitochondrial membrane potential , annexin 5 , and caspase activation were simultaneous monitored in individual cells during in vitro apoptosis . ^^^ Apoptotic B cells showed dramatic Bax upregulation and slight Bcl 2 decreases accompanied by decreased mitochondrial membrane potential and increased activated caspase 3 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , leptin changed the copy numbers of Bax and Bcl 2 mRNAs in the cultured cells as shown by real time quantitative RT PCR , although the effect was not consistent . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Asiatic acid treatment triggered the mitochondrial apoptotic pathway indicated by changing Bax / Bcl 2 ratios , cytochrome c release , and caspase 9 activation , but it did not act on Fas / Fas ligand pathways and the activation of caspase 8 . ^^^ Moreover , U 0126 and ERK siRNA inhibition completely suppressed asiatic acid induced Bcl 2 phosphorylation and Bax up regulation , and caspase 9 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The major antiapoptotic family members , Bcl 10 ( L ) and Bcl 2 , and the major proapoptotic proteins , Bax and Bak , show distinct temporal and spatial patterns of expression in the developing brain . ^^^ Targeted deletions of Bcl 10 ( L ) and Bcl 2 as well as Bax and Bak have proven to be important tools in delineating the process of cell death in the nervous system . ^^^ Bax , Bcl 10 ( L ) , and Bcl 2 regulate the apoptotic response to neurotrophic factor deprivation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To investigate the apoptosis of implanted primary gastric cancer cells in nude mice induced by resveratrol and the relation between this apoptosis and expression of bcl 2 and bax . ^^^ We observed the morphologic alterations by electron microscope , measured the apoptotic rate by TUNEL staining method , detected the expression of apoptosis regulated genes bcl 2 and bax by immunohistochemical staining and PT PCR . ^^^ The density of bcl 2 mRNA in 0 . 2 mL normal saline solution , 1 , 500 mg / kg DMSO , 500 mg / kg resveratrol , 1 , 000 mg / kg resveratrol , and 1 , 500 mg / kg resveratrol decreased progressively and the density of bax mRNA in 0 . 2 mL normal saline solution , 1 , 500 mg / kg DMSO , 500 mg / kg resveratrol , 1 , 000 mg / kg resveratrol , and 1 , 500 mg / kg increased progressively with elongation of time by RT PCR . ^^^ This apoptosis may be mediated by down regulating apoptosis regulated gene bcl 2 and up regulating the expression of apoptosis regulated gene bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was assessed in various types of gliomas and was defined by the apoptotic index ( IA ) and shown immunohistochemically with using Bcl 2 , Bax and Bcl 10 antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein expression in human optic nerve axons in the eyeballs after severe trauma and in the eyes with absolute glaucoma . ^^^ The aim of our study was to evaluate the immunohistochemical expression of Bcl 2 and Bax protein in optic nerve axons after severe eyeball injury and in the eyes with absolute glaucoma . ^^^ The immunohistochemical reaction was performed with Bcl 2 and Bax protein antibodies and by the LSAB technique . ^^^ The optic nerve axons in glaucomatous eyeballs showed statistically significant higher Bax protein expressions than those of Bcl 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bortezomib targets pathways relevant to tumor progression and therapy resistance and can directly modulate expression of cyclins , p27kip1 , p 53 , nuclear factor kB , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AA also markedly increased intracellular reactive oxygen species ( ROS ) level and enhanced the expression of Bax but not Bcl 2 protein in the cells . ^^^ These results suggest that AA induced apoptosis may be mediated through generation of ROS , alteration of Bax / Bcl 2 ratio and activation of caspase 3 , but p 53 independent . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of L tetrahydropalmatine on the expressions of bcl 2 and bax in rat after acute global cerebral ischemia and reperfusion . ^^^ To investigate the effects of L Tetrahydropalmatine ( L THP ) on the expressions of bcl 2 , bax and neuronal apoptosis after cerebral ischemia and reperfusion , 60 Wistars rats were randomly divided into 3 groups : sham operation group ( group S , n = 20 ) , ischemic reperfusion group treated with saline ( group 1 , n = 20 ) and ischemia reperfusion group treated with L THP ( group T , n = 20 ) . ^^^ The expression of bcl 2 and bax mRNA was detected by in situ hybridization and reverse transcriptional polymerase chain reaction ( RT PCR ) . ^^^ Compared with group S , the expression of bcl 2 and bax mRNA in group 1 was increased significantly ( P < 0 . 01 ) , and the number of apoptotic neurons increased either ( P < 0 . 01 ) . ^^^ After L THP treatment , the expression of bcl 2 mRNA was up regulated ( P < 0 . 01 ) and that of bax mRNA was down regulated ( P < 0 . 01 ) ; the number of apoptotic neurons was decreased ( P < 0 . 01 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of survivin and bax / bcl 2 in peroxisome proliferator activated receptor gamma ligands induces apoptosis on human myeloid leukemia cells in vitro . ^^^ Reverse transcription PCR and western blot analyses demonstrated that both survivin and bcl 2 expression were downregulated markedly , while bax expression was upregulated concurrently when apoptosis occurred . ^^^ We therefore conclude that 15d PGJ 2 and TGZ have significant apoptosis effects on K 562 and HL 60 cells in vitro , and that upregulation of bax as well as downregulation of survivin and bcl 2 expression may be the important apoptosis inducing mechanisms . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Serenoa repens treatment modifies bax / bcl 2 index expression and caspase 3 activity in prostatic tissue from patients with benign prostatic hyperplasia . ^^^ We tested the effect of this drug on molecular mechanisms associated with apoptosis , such as the Bax to Bcl 2 expression ratio and caspase 3 activity , in prostatic tissue from men with symptomatic BPH treated for 3 months before surgery . ^^^ Bax and Bcl 2 expression , and caspase 3 activity were determined by Western blot in 15 controls and 10 patients , and reported in blinded fashion . ^^^ RESULTS : The Bax to Bcl 2 ratio , which is used as an apoptotic index , was significantly increased in the prostatic tissue of treated patients . ^^^ CONCLUSIONS : Permixon increased molecular markers involved in the apoptotic process , ie the Bax to Bcl 2 expression ratio and caspase 3 activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Freshly obtained erythrocyte lysed bone marrow samples from 150 elderly patients ( > 65 years ) with de novo AML and 30 younger AML patients were analyzed using a 4 color immunofluorescence technique for quantitative expression of proteins associated with apoptosis ( bcl 2 , bax , APO2 . 7 ) and MDR ( P gp , MRP , LRP ) in 3 blast cell subpopulations , defined according to their maturation stage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To elucidate molecular events in the apoptosis , protein expressions of Bcl 2 , Bax , Fas , and FasL were measured by western blotting using specific antibodies in HL 60 cells . ^^^ The level of Bcl 2 indicated down regulation , but the Bax , Fas , and FasL expression showed up regulation as compared with the untreated control cells . ^^^ These data support the idea that endomorphins induce apoptosis in HL 60 cells through the activation of the Bcl 2 Bax and the Fas FasL pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
FL5 . 12 cells were stably transfected with antiapoptotic BCL 2 alone or in combination with proapoptotic BAX or nonfunctional mutant BAX , thereby creating various levels of imbalance within the BCL 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In quantitative RT PCR , bax mRNA was constantly expressed to similar degree in embryos of non diabetic and diabetic rats , while the expression of bcl 2 mRNA was significantly decreased in diabetic rat embryos compared to non diabetic rat embryos . ^^^ Immunohistochemical studies revealed that , in mirror sections , the staining of Bax and activated caspase 3 were observed in the TUNEL positive cell area , but the expression of Bcl 2 in these apoptotic cells was generally too low to be detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to understand the mechanism of apoptosis induction by S . lomentaria , we examined the changes of Bcl 2 and Bax protein expression levels . ^^^ The EtOAc fraction reduced Bcl 2 , an antiapoptotic protein , but increased Bax , a proapoptotic protein , in a dose dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MtDNA depleted cells show a 3 4 fold increased proapoptotic proteins ( Bax , BAD and Bid ) , markedly increased antiapoptotic Bcl 2 , and reduced processing of p 21 Bid to active tBid . ^^^ Inhibition of apoptosis closely correlated with sequestration of Bax , Bid and BAD in the mitochondrial inner membrane , increased Bcl 2 and Bcl 10 ( L ) , and inability to process p 21 Bid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Western immunoblot analysis showed down regulation of the bcl 2 protein and up regulation of the bax protein in the artesunate treated HUVEC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Therefore , the aim of our study was to examine the expression of p 53 , p 21 , bcl 2 , bax , and caspase 3 in association with DNA strand breaks in bronchial and alveolar epithelial cells in lung specimens from IPF patients and control subjects . ^^^ PATIENTS AND METHODS : We examined by immunohistochemistry the expression of p 53 , p 21 , bax , bcl 2 , and caspase 3 in association with DNA strand breaks detected by terminal deoxynucleotide transferase mediated deoxyuridine triphosphate biotin nick end labeling ( TUNEL ) in bronchial and alveolar epithelial cells in lung specimens taken by biopsy in 12 IPF patients and 10 control subjects . ^^^ CONCLUSIONS : These results confirm that apoptotic hyperplastic epithelial cells are present in patients with IPF and that the expression of p 53 , p 21 , bax , and caspase 3 appears to be up regulated and that of bcl 2 down regulated in these cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumors volume was measured weekly , and after 6 weeks of treatment with GRP antagonists the tumors were analyzed by Western blot assays for the expression of vascular endothelial growth factor ( VEGF ) , protein kinase C ( PKC ) alpha , the anti apoptotic protein Bcl 2 and the pro apoptotic protein Bax . ^^^ The relative ratio of Bcl 2 : Bax was also diminished by around 70 % by both analogs , indicating a net apoptotic gain and the efficacy of treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlation between expression of gastrin , somatostatin and cell apoptosis regulation gene bcl 2 / bax in large intestine carcinoma . ^^^ AIM : To explore the correlation between expression of somatostatin ( SS ) , gastrin ( GAS ) and cell apoptosis regulation gene bcl 2 / bax in large intestine carcinoma . ^^^ Immunohistochemical staining for bcl 2 , bax , GAS , SS was performed according to the standard streptavidin biotin peroxidase ( S P ) method . ^^^ Bax and bcl 2 protein expressions in different GAS and SS expression groups of large intestine carcinoma were assessed . ^^^ CONCLUSION : The regulation and control of gastrin , somatostatin in cell apoptosis of large intestine carcinoma may be directly related to the abnormal expression of bcl 2 , bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As expected ( ) gossypol induced complete cytochrome c release from mitochondria , increased caspases 3 and 9 activity , and caused apoptotic death without affecting protein levels of Bcl 2 , Bcl 10 ( L ) , Bax , and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed increased levels of phospho Sek 1 and phospho c Jun NH 2 kinase ( JNK ) in addition to total Ask 1 , GADD45beta , and Sek 1 . alpha TOS also altered JNK specific phosphorylation of Bcl 2 and Bim in a manner consistent with enhanced mitochondrial translocation of Bax and Bim . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EGCG decreased the expression of antiapoptotic protein Bcl 2 but increased proapoptotic protein Bax in these cells . ^^^ The increased ratio of Bax / Bcl 2 proteins after EGCG treatment may have resulted in increased release of cytochrome c from mitochondria into cytosols , increased expression of Apaf 1 , and activation of caspase 3 and poly ( ADP ribose ) polymerase , which may lead to apoptosis in MDA MB 468 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This review notably summarizes the present knowledge of the ( de ) regulation of the effects of androgens , p 53 , Bcl 2 , Bcl xL , Bax , Akt , PTEN , Par 4 , clusterine , caspases and NF kappaB in prostate adenocarcinoma cell lines and provides an appraisal of their therapeutic potential . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results also show that Bcl 2 , Bcl 10 ( L ) and Bax protein levels and heterodimerization are selectively regulated by NMDA and non NMDA receptor stimulation . ^^^ Striatal cell death induced by QUIN is mediated by an increase in Bax and a decrease in Bcl 2 protein levels , leading to reduced levels of Bax : Bcl 2 heterodimers . ^^^ In contrast , changes in Bax protein levels are not required for KA induced apoptotic cell death , but decreased levels of both Bax : Bcl 2 and Bax : Bcl 10 ( L ) heterodimer levels are necessary . ^^^ Intrastriatal grafting of a BDNF secreting cell line counter regulated p AKT , Bcl 2 , Bcl 10 ( L ) and Bax protein levels , prevented changes in the heterodimerization between Bax and pro survival proteins , and blocked caspase 3 activation induced by excitotoxicity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immediate early gene c Jun , the proapoptotic Bcl 2 family member Bax and the apoptosome constituent Apaf 1 have been shown previously to play major roles in the induction or execution of the apoptosis cascade . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of regulating molecules was analyzed by Western blotting for p 53 , p 21 ( WAF1 / CIP1 ) , and the Bcl 2 family , such as Bcl 2 , Bax , and Bcl 10 ( L / s ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Docosahexaenoic acid from a cultured microalga inhibits cell growth and induces apoptosis by upregulating Bax / Bcl 2 ratio in human breast carcinoma MCF 7 cells . ^^^ Western blot studies further show that aDHA did not modulate the expression of proapoptotic Bax protein but induced the downregulation of anti apoptotic Bcl 2 expression time dependently , causing increases of Bax / Bcl 2 ratio by 303 . 4 % and 386 . 5 % after 48 and 72 h incubations respectively with the fatty acid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study examined genistein induced apoptosis through the regulation of bcl 2 and bax expression in the presence of estrogen . ^^^ DNA ladder analysis and Western blot analysis of bcl 2 , bax , cyclin B ( 1 ) , p 21 , and p 53 were carried out . ^^^ Downregulation of bcl 2 and upregulation of bax by genistein were observed . ^^^ Bcl 2 / bax ratios were decreased by genistein treatment in the presence or absence of estrogen in female rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The object of our study was to evaluate the expression of the Bcl 2 protein family ( Bcl 2 , Bak , Bax ) , p 53 , proliferating cell nuclear antigen ( PCNA ) , and Ki 67 protein immunoexpression as well as the correlation between the examined markers and some clinicopathological features in papillomas and cancers of conjunctiva and eyelid . ^^^ In the SCP group , p 53 protein expression was observed in 30 cases ( 66 . 6 % ) , Ki 67 in 14 ( 31 . 1 % ) , PCNA in 44 ( 97 . 8 % ) , Bcl 2 in 24 ( 53 . 3 % ) , Bak in 28 ( 62 . 2 % ) , Bax in 31 ( 68 . 9 % ) , and Bcl xl in 11 ( 100 % ) . ^^^ In the SCC group , p 53 protein expression was evaluated in 8 cases ( 72 . 8 % ) , Ki 67 in 2 ( 18 . 2 % ) , PCNA in 8 ( 72 . 7 % ) , Bcl 2 in 5 ( 45 . 4 % ) , Bax and Bak both in 10 ( 90 . 9 % ) , and Bcl xl in 100 % . ^^^ In the BCC group , p 53 protein expression was estimated in 23 cases ( 85 . 1 % ) , Ki 67 in 13 ( 48 . 1 % ) , PCNA in 26 ( 96 . 2 % ) , Bcl 2 in 13 ( 48 . 1 % ) , Bak in 21 ( 77 . 8 % ) , Bax in 22 ( 81 . 5 % ) , and Bcl xl in 23 ( 85 . 2 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl 2 / Bax family protein BimEL . ^^^ Recently , we showed that HN binds to and inhibits Bax , a proapoptotic Bcl 2 family protein , suggesting a mechanism for HN action . ^^^ In this study , we identified Bim , a Bcl 2 homology 3 only member of the Bcl 2 / Bax family , as an additional HN target protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Multiple apoptotic stimuli induce conformational changes in Bax , a proapoptotic protein from the Bcl 2 family and its deficiency is a frequent cause of chemoresistance in colon adenocarcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our evaluation of expression levels of Bcl 2 family members showed increased expression of pro apoptotic Bak and Bax in diabetic rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell cycle and early apoptosis were determined by propidium iodide and / or Annexin 5 staining , and the levels of Bcl 2 and Bax were analyzed by flow cytometry . ^^^ Bcl 2 level slightly decreased ; in contrast , Bax level elevated markedly , which resulted in a significant decrease of Bcl 2 / Bax ratio . ^^^ CONCLUSION : PA may induce cell death on hepatocytes via mitochondria mediated apoptosis by reducing the level of Bcl 2 / Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic markers in lung tissue sections ( TUNEL ) and in TIIcells ( cell death detection ELISA , Bax , Bcl 2 , mitochondrial membrane potential , and late and early apoptotic cells ) were measured using commercially available kits . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The potentiation of As2O3 provoked apoptosis involved the increased disruption of mitochondrial transmembrane potential , increased caspase 3 activation and cytochrome c release from mitochondria , increased Bax and Bid activation , and attenuation of 27 kDa heat shock protein ( HSP 27 ) expression ; the potentiation was prevented by Bcl 2 overexpression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fucoxanthin treatment caused cleavages of procaspase 3 and poly ( ADP ribose ) polymerase without any effect on the protein level of Bcl 2 , Bcl 10 ( L ) , or Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We identified early proapoptotic transcriptional changes , including upregulation of proapoptotic Bax and downregulation of antiapoptotic Bcl xL , Bcl 2 , and Bcl w , using Affymetrix DNA microarrays . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regarding the antiapoptotic mechanisms , we have previously demonstrated that under staurosporine treatment , HalphaA and HalphaB crystallins can interact with Bax and Bcl XS , proapoptotic members of the Bcl 2 family , to sequester their translocation into mitochondria , and thus prevent the staurosporine induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of bcl xL mRNA was significantly decreased in the OP treated groups , whereas the expressions of bcl 2 and bax mRNA were not significantly changed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MPA induced differentiation , unlike that induced by retinoic acid , caused a less extensive neurite outgrowth and branching ( similar to that observed in cultured HPRT deficient dopaminergic neurons ) and involved up regulation of p 53 , p 21 and bax , and bcl 2 down regulation without p 27 protein accumulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was assessed by DNA laddering , localizing apoptotic bodies using immunofluorescent labeling of DNA fragments ( the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method ) , and immunohistochemical assessment of apoptosis markers bcl 2 , bcl 10 , and bax . ^^^ Immunostaining for bcl 2 was higher in hCG and progesterone treated cycles , whereas bax expression was decreased and bcl 10 immunostaining was not different between treatments . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , immunocytochemical data and Western blot analysis revealed that , unlike flavonol derivatives , the expression of Bcl 2 was markedly up regulated , and that the expression of Bax and activation of caspase 3 were strongly inhibited by this flavanone derivative , thereby implicating antioxidant activity related anti apoptotic mechanisms of 2 ' , 4 ' , 7 trihydroxyflavanone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of p 53 , Bax , Bcl 2 , NFkB , cytochrome c , caspase 3 as well as cleavage of caspase 3 were measured by western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , C . rhizoma treatment ( 100 microg / ml ) increased levels of BAX and CASPASE 3 , and decreased levels of BCL 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although the precise mechanism that leads to the permeabilization of mitochondria is still unclear , the activation of multidomain pro apoptotic proteins of the Bcl 2 family , such as Bax and Bak , is evidently crucial . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , exposure of VSMCs to high glucose markedly increased the abundance of Bcl 2 and Bcl xl mRNAs compared with treatment with normal glucose , while expression of bax and IAP 1 mRNA remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prognostic value of Bax , Bcl 2 , p 53 , and TUNEL staining in patients with radically resected ampullary carcinoma . ^^^ AIMS : To examine the prognostic significance of Bax , Bcl 2 , and p 53 protein expression and the apoptotic index in a large cohort of uniformly treated patients with radically resected ampullary cancer . ^^^ Expression analysis for p 53 , Bax , and Bcl 2 was performed by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A reverse transcriptase polymerase chain reaction ( RT PCR ) showed no specific changes of apoptosis related gene expression ( 1 . e . , bax , ICE alpha , beta , bcl 2 , and c myc ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : We examined the expression pattern of bax , bcl 2 , bcl xL , mtd , and bcl w both at messenger RNA ( mRNA ) and protein level in the brain tissues during the formation of epilepsy with kindling model in adult rats , which has been the most acceptable form of experimental model of human epilepsy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of SH SY5Y cells with MPP ( + ) caused the loss of cell viability , and condensation and fragmentation of nuclei , which was associated with the elevation of ROS level , the increase in Bax / Bcl 2 ratio , and the activation of caspase 3 . ^^^ Our results suggested that the protective effects of SA on MPP ( + ) induced cytotoxicity may be ascribed to its antioxidative properties and anti apoptotic activity via regulating the expression of Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic ( Bcl 2 and Bcl 10 ( L ) ) and proapoptotic ( Bax and Bad ) proteins were expressed in neonatal rat hearts of both groups . ^^^ Prenatal cocaine exposure decreased levels of Bcl 2 in 21 day and increased Bax in 21 and 30 day rat hearts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
More biochemical features of apoptotic death , such as upregulation of Bax : Bcl 2 ratio , calpain activity , and caspase 3 activity , were assessed by Western blot analysis . ^^^ However , a pretreatment with 100 microM or 200 microM DXM protected T98G cells against TMZ induced apoptosis , concomitantly decreasing Bax : Bcl 2 ratio , calpain activity , and caspase 3 activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fas , FasL , Bcl 2 , and Bax in the endometrium of rhesus monkey during the menstrual cycle . ^^^ To study possible role and regulation of apoptosis occurred in primate endometrium , the expression of apoptosis related molecules , Fas , FasL , B cell lymphoma / leukaemia 2 ( Bcl 2 ) , and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunohistochemistry and Western blot . ^^^ The results showed that the expressions of Fas , Fas ligand ( FasL ) , Bcl 2 , and Bax were co localized predominantly in the epithelial cells of the endometrium . ^^^ The coordinated expression of Fas , FasL , Bcl 2 , and Bax in the cycling endometrium of the rhesus monkey suggests that the cyclic changes in endometrial growth and regression may be regulated by the balance of these factors under the action of ovary steroids . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , the results showed that acacetin induced apoptosis was accompanied by up regulation of Bax and p 53 , down regulation of Bcl 2 , and cleavage of Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , we have shown that transient overexpression of Bcl 2 protected the HUVECs from maspin mediated apoptosis , and the presence of both maspin and Bax accelerated the apoptosis process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Specifically , we observe that Abeta significantly reduces expression of antiapoptotic Bcl w and Bcl 10 ( L ) , mildly affects expression of bim , Bcl 2 , and bax , but does not alter expression of bak , bad , bik , bid , or BNIP3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
T 3 treatment resulted in increase in mitochondrial Bax and Bak together with decreased mitochondrial Bcl 2 . ^^^ In contrast to Bax Bcl 2 family proteins , some other MTP components were either not induced by T 3 ( e . g . voltage dependent anion channel ) or were induced , but were not involved in Cyclosporin A sensitive MTP gating ( e . g . ^^^ Cyclophilin D and adenine nucleotide translocase 2 ) Hence , TH induced mitochondrial uncoupling may be ascribed to low conductance MTP gating mediated by TH induced increase in mitochondrial proapoptotic combined with a decrease in mitochondrial antiapoptotic proteins of the Bax Bcl 2 family . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We performed cortical cell counts , determinations of neuronal size and Western analyses of the apoptosis related proteins , Bcl 2 and Bax , in rat pups who were raised in chronic hypoxia ( FiO 2 9 . 5 % ) beginning on postnatal day 3 ( P 3 ) and extending for either 10 ( P 13 ) or 30 ( P 33 ) days . ^^^ Western blot analyses of Bcl 2 and Bax protein levels demonstrated a ratio favorable to Bax at multiple time points during the period of hypoxic exposure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein p 53 is able to reduce bcl 2 and promote bax expression . ^^^ This study focused on the immunohistochemical detection of the expression levels of Bcl 2 family regulators ( anti apoptotic Bcl 2 and Bcl XL , pro apoptotic Bcl Xs and Bax ) , p 53 , and PCNA as a marker of proliferation , together with the evaluation of the level of apoptosis in human embryos ( anlage of limbs , axial skeleton , metanephros , and intestine ) . ^^^ The role of pro apoptotic members of Bcl 2 family remains ambiguous , as TUNEL positive cells are both Bax / Bcl Xs positive and negative . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bovine herpesvirus VP 22 induces apoptosis in neuroblastoma cells by upregulating the expression ratio of Bax to Bcl 2 . ^^^ Further , analyses of caspase 3 activity and apoptosis suggested that BVP 22 induces apoptosis in host tumor cells by upregulating the expression ratio of Bax to Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis was used to determine the protein expression of cancer suppressor genes , p 53 ( wt ) and Bax , and the proto oncogene , Bcl 2 in LNCaP cells following treatment with oridonin . ^^^ Oridonin up regulated p 53 and Bax and down regulated Bcl 2 expression in a dose dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Microtubule inhibitor D 24851 induces p 53 independent apoptotic cell death in malignant glioma cells through Bcl 2 phosphorylation and Bax translocation . ^^^ D 24851 treatment induced phosphorylated Bcl 2 and translocated Bax from the cytoplasm to the mitochondria , resulting in apoptotic cell death . ^^^ Our results indicated that D 24851 effectively induces apoptosis through Bcl 2 phosphorylation and Bax translocation in human malignant glioma cells in a p 53 independent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We therefore generated a stable transfected beta cell line ( INS 1 ) overexpressing human TXNIP and found that TXNIP overexpression induced apoptosis as assessed by Bax , Bcl 2 , caspase 3 , and cleaved caspase 9 as well as Hoechst staining . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The best characterized antiapoptotic Bcl 2 mechanism of action involves direct binding to proapoptotic proteins , e . g . , Bax , inhibiting their ability to oligomerize and form pores in the mitochondrial outer membrane , through which soluble mitochondrial proapoptotic proteins , e . g . , cytochrome c , are released into the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of pro apoptotic gene , Bax , was increased and the expression of anti apoptotic gene , Bcl 2 , was decreased by CR treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein levels of two oncogenes , Bax and Bcl 2 , remained unchanged , when PBMC were incubated with KH 1060 , anti TNF or their combination in the absence of LPS , while , in its presence , an increase was registered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transactivation of p 53 in MCF 7 cells also led to increase in expression of Bax , proapototic Bcl 2 family member , triggering mitochondrial pore opening , and PIG 3 ( p 53 induced gene 3 product ) , and also generation of intracellular reactive oxygen species ( ROS ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cleavage of the proapoptotic Bcl 2 proteins , such as Bad and Bax to produce their truncated forms , and the cleavage of the antiapoptotic Bcl 2 proteins , such as Bcl 2 and Bcl XL , into their potent pro apoptotic fragments were detected in our study . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Silymarin induces apoptosis primarily through a p 53 dependent pathway involving Bcl 2 / Bax , cytochrome c release , and caspase activation . ^^^ The constitutive expression of antiapoptotic proteins Bcl 2 and Bcl xl were decreased after silymarin treatment , whereas the expression of the proapoptotic protein Bax was increased . ^^^ There was a shift in Bax / Bcl 2 ratio in favor of apoptotic signal in silymarin treated cells , which resulted in increased levels of cytochrome c release , apoptotic protease activating factor 1 , and cleaved caspase 3 and poly ( ADP ribose ) polymerase in JB 6 C141 cells . ^^^ The shift in Bax / Bcl 2 ratio was more prominent in p53+ / + fibroblasts than in p 53 / cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A study of Bcl 2 / Bax expression and keratinocyte rescue effects . ^^^ Apoptosis and alterations in Bcl 2 and Bax messenger RNA ( mRNA ) and protein expression were examined in cultured human epidermal melanocytes following UVB irradiation ( 50 mJ / cm ) . ^^^ Different wavelengths within the UVB spectrum induced diverse response patterns of Bcl 2 and Bax mRNA and had different apoptotic power . ^^^ Both Bcl 2 and Bax mRNA were upregulated to preserve protein levels and only a slight increase in apoptosis was noted 24 h after UVB ( lambda > 305 nm ) . ^^^ Increasing UVB between 280 and 305 nm enhanced apoptosis and upregulated Bcl 2 , whilst Bax mRNA was unaltered . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed that induction of apoptosis by ways of inhibition of the bcl 2 , loss of the Bax , and upregulation of Fas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Different protein regulators of apoptosis , such as Bcl 2 , BclX , and Bax have an influence on the rate of apoptosis in various tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic proteins , Bcl 2 and Bcl xl , were downregulated by GSP , whereas the expression of the pro apoptotic protein , Bax , and the levels of cytochrome c release , Apaf 1 , caspase 9 , and cleaved caspase 3 ( p 19 and p 17 ) were markedly increased in JB 6 C141 cells . ^^^ The downregulation of Bcl 2 and upregulation of Bax were also observed in wild type p 53 ( p53+ / + ) fibroblasts but was not observed in their p 53 deficient counterparts . ^^^ These data clearly demonstrate that GSP induced apoptosis is p 53 dependent and mediated through the Bcl 2 , Bax , and caspase 3 pathways . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using a Bax dependent membrane permeabilization assay , we show that peptides corresponding to the BH 3 domains of Bcl 2 family `` BH 3 only ' ' proteins have dual functions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We and others , have previously reported that in a stable HT 22 neuronal cell line , glutamate induces apoptosis as indicated by DNA fragmentation and up and down regulation of Bax ( pro apoptotic ) , and Bcl 2 ( anti apoptotic ) genes respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We now report that C . trachomatis infection leads to degradation of Bik , Puma , and Bim , three upstream proapoptotic BH 3 only proteins of the Bcl 2 family that can transmit death signals to mitochondria by inhibiting the Bcl 2 antiapoptotic proteins and / or activating the Bcl 2 proapoptotic members , such as Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , AMPH also caused increased expression of p 53 and Bax at both transcript and protein levels ; in contrast , Bcl 2 levels were decreased after the AMPH injections . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Left ventricular endothelial cells ( LVECs ) were isolated and levels of the anti apoptotic protein Bcl 2 and the pro apoptotic protein Bax were measured . ^^^ Antioxidant supplementation caused a fourfold increase in Bcl 2 ( P < 0 . 05 ) with no change in Bax ( P > 0 . 05 ) . ^^^ Bcl 2 : Bax was increased sixfold with antioxidant supplementation compared to non supplemented animals ( P < 0 . 05 ) . ^^^ Exercise training had no significant effect on Bcl 2 , Bax or Bcl 2 : Bax either alone or combined with antioxidant supplementation ( P > 0 . 05 ) compared to non supplemented animals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we investigated whether GnRHa caused apoptosis in mature corpus luteum of superovulated rats and also examined the expression of apoptosis related molecules ( Fas , Fas ligand ( FasL ) , Bcl 2 , Bax ) . ^^^ FasL expression decreased and Bax expression increased in structural luteolysis induced by GnRHa as well as Brom+PRL treatment , although Fas and Bcl 2 expression did not change throughout the luteal phase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of BCL 2 and BAX was quantified using real time PCR analysis . ^^^ Low BCL 2 : BAX ratios were observed in all samples when compared with pre culture controls . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased cisplatin sensitivity in MAD 2 transfectants was associated with mitotic arrest and activation of apoptosis pathway evidenced by the increased mitotic index and apoptosis rate as well as decreased Bcl 2 and Bax ratio and expression of cleaved poly ( ADP ribose ) polymerase and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study explores the roles of Bax and other Bcl 2 family members play in arsenic trioxide ( As ( 2 ) O ( 3 ) ) induced apoptosis . ^^^ Stable overexpression of Bcl 2 in IM 9 cells ( IM 9 / Bcl 2 ) inhibited As ( 2 ) O ( 3 ) mediated Bax activation and apoptosis , and this inhibition could be partially averted by cell permeable Bid Bcl 2 homology ( BH ) 3 peptide . ^^^ These data suggest that As ( 2 ) O ( 3 ) might exert the cell killing in part by inducing Bax activation through a Bcl 2 suppressible pathway in hematopoietic cells that is caspase independent and intracellular ROS regulated . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis ( 7 amino actinomycin D , Annexin , single stranded DNA and caspase ) , Bcl 2 , Bax and p 53 were assessed in cells obtained from bronchial bushing and bronchoalveolar lavage from ex and continuing smokers with COPD , and nonsmoking controls , using flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , delphinidin induced apoptotic cell death was accompanied by up regulation of Bax and down regulation of Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we assessed quercetin regulation of HT 29 and SW 480 cell apoptosis and the influence of quercetin on the protein expression of ErbB 2 , ErbB 3 , Akt , Bax and Bcl 2 . ^^^ Western blot analysis of cell lysates revealed that Bcl 2 levels decreased dose dependently in cells treated with quercetin , but Bax remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pseudolaric acid B induces apoptosis through p 53 and Bax / Bcl 2 pathways in human melanoma A 375 S2 cells . ^^^ There was a decrease in the expression of anti apoptotic Bcl 2 and Bcl xL proteins , whereas pro apoptotic Bax was increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax is a death promoter member of the bcl 2 family which plays a central role in the regulation and commitment to programmed cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The formalin fixed , paraffin embedded tissues of CNS and non CNS DLBCL ( 20 cases each ) were studied by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) and immunohistochemistry , using antibodies against single stranded DNA ( ssDNA ) , cleaved caspase 3 , bcl 2 , bax , p 53 , Fas and Ki 67 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumors were screened for bax ( G ) 8 mutations and evaluated immunohistochemically for bax , bcl 2 , and p 53 protein expression and apoptotic ( M 30 cytoDEATH ) and proliferative ( Ki 67 ) indexes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , issuing from our previous observations , we tried to compare their ways of expression with those of Bcl 2 ( antiapoptotic effect ) and Bax ( proapoptotic function ) proteins . ^^^ Such localization is also typical for Bcl 2 protein whereas Bax positive cells are present mostly in branches of the ureteral bud . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the 6th and 8th week of intrauterine development we observed isolated TUNEL positive epithelial cells only and this was accompanied by the disperse presence of PCNA as well as by all the studied proteins : Bcl 2 , Bax , Bcl XL , c myc , N myc , p 53 , p 63 and p 73 . ^^^ In the presence of Bcl 2 , Bax , Bcl XL , p 63 and p 73 we did not find any dramatic changes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Glutamate excitotoxicity decreased bcl 2 , bcl 10 ( L ) , and bax mRNA levels , . ^^^ NMDA increases Bcl 2 and Bcl 10 ( L ) protein levels and decreases Bax protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regarding apoptotic cell death induced by cisplatin , serum deprivation and contact inhibition was increased by overexpression of 1 kappaBalpha , whereas overexpression of bcl 2 inhibited the apoptotic cell death . 1 kappaBalpha overexpression increased Bax expression and decreased cIAP 1 and 2 expression compared to vector transfected cells , but did not alter SAPK / JNK activity in the presence or absence of cisplatin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , apoptotic +SA cells showed a paradoxical decrease in mitochondrial levels of pro apoptotic proteins Bid , Bax , and Bad , and a corresponding increase in mitochondrial levels of anti apoptotic proteins , Bcl 2 and Bcl xL , suggesting that mitochondrial membrane stability and integrity might actually be enhanced for a limited period of time following acute tocotrienol exposure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The process of apoptosis ( assessed by Annexin 5 and ssDNA staining ) , as well as Bcl 2 , Bax , p 53 , IL 2 , IL 4 and receptors IL 7R , IL 4R and IL 2Rgamma were investigated in PHA stimulated peripheral blood derived T cells , using flow cytometry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , we found that overexpression of p33ING2 significantly downregulated the expression of Bcl 2 after UVB irradiation , resulting in an increased Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase in apoptosis was associated with a decrease in Bcl 2 expression , an increase of Bax and an activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiproliferative effects of Citrus reticulata Blanco ( CR ) extract , the immature tangerine peel , on human gastric cancer cell line SNU 668 were evaluated using 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide assay , 4 , 6 diamidineo 2 phenylindole staining , terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay , reverse transcription polymerase chain reaction expressions of BCL 2 , BAX and CASP 3 genes , caspase 3 activity , and immunocytochemistry of caspase 3 . ^^^ CR ( 50 microg / ml ) reduced the expression of BCL 2 , whereas the expression of BAX and CASP 3 was increased compared with the control group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twenty five hyperplastic polyps , 25 serrated adenomas , and 25 tubulovillous adenomas resected from the colons of 75 patients were studied by immunohistochemical staining using monoclonal antibodies against MIB 1 , Bcl 2 , Bax , p 53 , and the TUNEL method for the detection of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we show that multidomain proapoptotic Bcl 2 family members Bax and Bak play a critical role in apoptosis induction by sulforaphane . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This increase in apoptosis was associated with a decrease in Bcl 2 and expression , an increase of Bax , and an activation of caspase 3 and caspase 9 . beta lapachone treatment markedly inhibited the activity of telomerase in a dose dependent fashion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Estrogen dose dependently increased the expression of Bcl 2 protein in MCF 7 , but not in MDA MB 231 cells , and suppressed the expression of Bax and cytochrome c induced by anticancer drugs in association with decreased apoptosis compared with the effect of each drug alone . ^^^ Estradiol decreased sensitivity to ADM , MMC , and TXL in MCF 7 and MDA MB 231 breast cancer cells , and this was associated in part with an increase in the amount of Bcl 2 protein , and decreases in levels of Bax and cytochrome c leading to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses revealed that CT induced an elevation of bcl xL but not bcl 2 or proapoptotic factors bax , bak , and bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in apoptosis was accompanied with decrease in the protein expression of Bcl 2 concomitantly increase in Bax , cytochrome c release , Apaf 1 , and cleavage of caspase 3 and PARP proteins . ^^^ Treatment of EGCG rich GTP in drinking water to 4T1 cells bearing BALB / c mice resulted in reduction of tumor growth accompanied with increase in Bax / Bcl 2 ratio , reduction in proliferating cell nuclear antigen and activation of caspase 3 in tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Caspase 3 and poly ( ADP ribose ) polymerase ( PARP ) activation and Bax and Bcl 2 expression were detected by Western blot analysis . ^^^ Bcl 2 expression was down regulated while Bax expression up regulated concurrently when the cells were treated with ponicidin for 24 48 h . ^^^ Therefore , we conclude that ponicidin has significant anti proliferation effects by induction of apoptosis on myeloid leukemia cells in vitro , down regulation of Bcl 2 , and up regulation of Bax , and that activation of caspase 3 and PARP may be an important apoptosis inducing mechanism . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein levels of Bax , Bcl 2 , and Caspase 3 were quantified by Western blot . ^^^ Bcl 2 was down regulated when U2OS cells were treated with azurin for 24 h , while Bax and caspase 3 were up regulated . ^^^ The induction of apoptosis by azurin may be closely associated with down regulation of Bcl 2 , up regulation of Bax , and activation of Caspase 3 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis of Bax , Bcl 2 , Bcl xL , and caspase 9 were performed . ^^^ Bax and caspase 9 protein expression increased significantly as oxalate concentrations increased , but Bcl 2 protein expression decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no significant difference in the expression levels of Bcl 2 , Bax , and phospho Akt in endothelial cells treated with anthracycline derivatives . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of NADPH oxidase , Akt , pAkt , Bcl 2 , Bax , IkappaB , caveolin 1 and eNOS was evaluated by Western blot analysis . 2 ET 1 significantly enhanced ROS generation and cell proliferation following 24 h incubation , both of which were prevented by BQ 788 or apocynin , consistent with the ability of ET 1 to directly upregulate NADPH oxidase . ^^^ Western blot analysis indicated that ET 1 alleviated homocysteine ( Hcy ) induced apoptosis , likely acting by antagonizing the Hcy induced decreases in Akt , pAkt , pAkt to Akt , Bcl 2 to Bax ratios and increases in Bax and caveolin 1 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of cell death in rat small intestine by ischemia reperfusion : differential roles of Fas / Fas ligand and Bcl 2 / Bax systems depending upon cell types . ^^^ We investigated the appearance of apoptosis at various time points ( 0 24 h ) of reperfusion after 1 h ischemia and the expression of various apoptosis related proteins , such as Bcl 2 , Bax , Fas , Fas ligand ( FasL ) , activated caspase 3 , and cytochrome c , immunohistochemically in rat small intestine . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Indeed , bortezomib induced increases of Bik and / or Bim in multiple cell lines but not notably of two other BH 3 only proteins ( Puma and Bid ) nor other family members ( Bax , Bak , Bcl 2 , and Bcl xL ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of pro apoptotic Bax was up regulated and anti apoptotic Bcl 2 was down regulated in only the G F treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR and immunoblot analyses indicated that Bax was dramatically upregulated while Bcl 2 was modestly increased ; however , the Bax / Bcl 2 ratio was significantly increased in denervated muscles relative to control muscles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neuronal damage was assessed by analyzing Bax , Bcl 2 , p 53 , and Mdm 2 proteins , activated caspases 3 positive and eosinophilic cells . ^^^ CONCLUSION : The present data show that hypothermia prevents an ischemia induced increase of the pro apoptotic protein Bax for as long as 28 days and increases the concentration of the antiapoptotic protein Bcl 2 up to 3 days compared to normothermic animals . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DMBA induced apoptosis , evaluated by the TUNEL assay , immunohistochemical analysis of activated caspase 3 , and the ratio of expression of pro apoptotic Bax to anti apoptotic Bcl 2 , was greater in rats fed NIH 31 diet relative to rats fed 5K96 diet at 24 h after treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bid are proapoptotic proteins of the Bcl 2 family that regulate the release of apoptogenic factors from mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The gene expressions of Bax , Bcl 2 , Fas , FasL , Caspase 1 , 2 , 3 , and 9 , p 53 , and Smac / DIABLO were examined using a quantitative real time polymerase chain reaction method , while nuclear factor kappaB activity was examined using an electrophoresis mobility shift assay with a nuclear factor kappaB consensus sequenced DNA probe . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MX 3350 1 decreased the levels of antiapoptotic Bcl 2 and Bcl XL , increased proapoptotic Bax , induced mitochondrial membrane permeabilization ( MMP ) , and cytochrome c release from mitochondria to cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Animals were then sacrificed , and TUNEL , anti active Caspase 3 , bcl 2 , and bax labeling were performed in paraffin embedded brain sections , allowing for comparative and quantitative analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of MUC 18 , Bcl 2 , Bax , caspase 3 , poly ( ADP ribose ) polymerase ( PARP ) , phospho ( Ser 473 ) AKT , and Ki 67 in the mouse prostate tumors were determined . ^^^ The doxazosin treatment did not further decrease the expression of an already low level of Bcl 2 in all prostate tumors , but it increased the expression of Bax , and the activation of caspase 3 , and the cleavage of a downstream substrate , PARP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RA repressed c fos mRNA expression in control and irradiated SiHa cultures , but did not repress bcl 10 ( L ) , p 53 , GADD 45 , p 21 , bax , bcl 2 , or mcl 1 mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Contemporarily , the expression of p27 / KIP1 , proapoptotic factor Bax increased , and expression of Bcl 2 decreased , which might cooperate to induce apoptosis . ^^^ Inhibiting ERK activation reduced the increased expression of p 27 and Bax , but had no effect on the decreased expression of Bcl 2 , suggesting the involvement of ERK activation in the SN induced increased expression of p 27 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis of the RGC 5 cells was associated with the activation of caspase 3 , 8 , and 9 , and increased levels of Bax with corresponding decreases in Bcl 2 levels and NF kappaB ( NF kappaB ) binding activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We reported recently that ( ) epigallocatechin gallate and quercetin inhibited H2O2 induced apoptosis through modulation of the expression of apoptosis related Bcl 2 and Bax in endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moxa smoke dose dependently induced internucleosomal DNA fragmentation , activation of caspases 3 , 8 and 9 , and slightly modified the expression of apoptosis related proteins ( Bcl 2 , Bad , Bax ) in HL 60 cells , but to much lesser extents than attained by positive controls ( UV irradiation , actinomycin D treatment ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prevalence of apoptosis was defined by TUNEL and ISOL staining and further characterized by immunohistochemical staining for caspase 3 , Bcl 2 , BCL 10 ( L ) , Bax , proliferating cell nuclear antigen ( PCNA ) , and Ki 67 . ^^^ The antiapoptotic proteins , Bcl 2 and BCL 10 ( L ) , demonstrated intense upregulation within and surrounding MFL cells , whereas pro apoptotic protein Bax expression was only seen at control level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
An increased ratio of bax to bcl 2 mRNA level with subsequent activation of caspase 3 was observed in high glucose or H2O2 exposed pericytes , which was also completely prevented by PEDF . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To examine apoptosis and mRNA levels of bcl 2 and bax in granulosa cells , follicles harvested 48 h after PMSG injection were cultured in serum free conditions . ^^^ The bcl 2 mRNA levels were similar in control and heat stressed granulosa cells ; bax mRNA levels were increased in heat stressed granulosa cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytochrome c release from mitochondria to the cytosol occurred after Bax translocation and the dephosphorylation of pS 70 Bcl 2 . ^^^ Taken together , we conclude that TGHQ facilitates ROS production , alters the post translational modification of Bcl 2 and subcellular localization of Bax , culminating in the release of cytochrome c and caspase activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The synthetic ligands significantly reduced or induced the expression of cyclins , p27Kip1 , p21Waf1 / Cip1 , MDM 2 , Bcl 2 , Bax , PARP , Caspase 3 , androgen receptors , etc . and did not affect the expression of the differentiation marker GFAP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The treatment with DNP also markedly reduced levels of Bcl 2 , inhibitor of apoptosis protein 1 , and inhibitor of apoptosis protein 2 and increased the level of Bax and cytochrome c release into cytoplasm and subsequent caspase 3 activation , which co occurred with increased apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we review the significance of apoptosis dysregulation in CLL , focusing on the role of Bcl 2 and related Bcl 2 family proteins , such as Bax and Mcl 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 overexpressing , Bax knockout , and caspase 9 deficient cells were partially resistant to cell death , suggesting the involvement of the intrinsic apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using reverse transcription polymerase chain reaction , expression Bcl 2 and Bax mRNA in blastocyst were evaluated in the GM CSF group and control group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax , Bcl 2 and caspase 3 in spared dorsal root ganglion after partial dorsal root rhizotomy ] . ^^^ OBJECTIVE : Investigating the expression of Bax , Bcl 2 and Caspase 3 in dorsal root ganglion after partial dorsal root rhizotomy in spared root . ^^^ On the 3rd , 7th and 14th days after partial dorsal rhizotomy , the DRG ( L 6 ) of 5 cats per batch were taken and made into frozen sections and were stained under the same condition using specific Bax , Bcl 2 and Caspase 3 antibody by ABC method . ^^^ The Bax , Bcl 2 and Caspase 3 positive large , medium and small sized neurons in DRG were counted in the 3 experiment groups and in the normal control group . ^^^ RESULTS : In the intact animals of the normal control group , the Bax , Bcl 2 and Caspase 3 immunoreactants were mainly distributed in medium , small neurons and a few large neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax and Bcl 2 was significantly increased after reperfusion , being slightly inhibited by the administration of nicorandil . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The changes of the biological response to the lymphatic transported 32P colloids in the inguinal lymph node ( ILN ) were dynamically observed , and the percentage of tumor cell apoptosis , and Apo2 . 7 , caspase 3 , Bcl 2 , Bax related gene expression were observed too . ^^^ In the course of irradiation induced apoptosis , the value of Bcl 2 / Bax was down regulated ; Apo2 . 7 and caspase 3 protein expression were prominently increased dose dependently . ^^^ The low dose 32P colloids may induce human pancreatic carcinoma Pc 3 implanted tumor cell apoptosis ; Apo2 . 7 , caspase 3 , Bcl 2 and Bax protein participated in regulating the process of irradiation induced cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , expression of the apoptosis regulating proteins Bax , Bak , Bid , Bcl w , Bcl 2 , XIAP and Mcl 1 was evaluated in B CLL lymphocytes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We studied whether Bcl 2 , Bcl xL , Mcl 1 , Bax as well as the Bcl 2 / Bax ratio and a combination of all ( antiapoptosis index , AAI ) are related to the frequency of malignant cells surviving the chemotherapy ( i . e . , minimal residual disease , MRD ) . ^^^ RESULTS : We found that Bcl 2 ( R = 0 . 55 , P = 0 . 002 ) , Bcl 2 / Bax ( R = 0 . 42 , P = 0 . 02 ) , and AAI ( R = 0 . 47 , P = 0 . 01 ) at diagnosis directly correlated with MRD after the first cycle of chemotherapy . ^^^ Taken together , these results directly explain why Bcl 2 / Bax and especially AAI ( P = 0 . 007 ) at diagnosis correlate with DFS . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic proteins p 53 , bax , bcl 2 , and the proliferation marker Ki 67 , are known to play an important role in UV exposed skin and carcinomas , therefore , these markers were assessed in AC and compared with normal lip and oral mucosa . ^^^ METHODS : AC ( n = 13 ) , normal lip ( n = 7 ) and oral mucosa ( n = 6 ) biopsies were stained immunohistochemically for p 53 , bax , bcl 2 and Ki 67 , to determine their expression and distribution . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chronic treatment of Vancouver diabetic fatty Zucker rats with GIP resulted in down regulation of Bax and up regulation of Bcl 2 in pancreatic beta cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was detected by terminal deoxynucleotidyl transferase end labelling ( TUNEL ) , as well as by immunostaining for caspase 3 , Bax and Bcl 2 . ^^^ The positive staining for caspase 3 was increased in the unstable plaques ( P = 0 . 0008 ) , while no difference in either Bax or Bcl 2 was found between groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , we analyzed cell apoptosis by measuring Bcl 2 and Bax protein level and DNA ladder assay . ^^^ After performing Cyclin B 1 RNAi , Cyclin B 1 , Cyclin A and Cdk 2 protein levels were found to be markedly downregulated , whereas Cdc 2 was almost unaffected ; S phase fraction increased significantly ; HeLa cell viability and cell colony forming ability were markedly diminished after the RNAi ; Bcl 2 was noticeably attenuated but Bax was hardly changed ; and HeLa cells displayed typical DNA ladder . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To characterize the mechanisms of apoptosis induction by proteasome inhibitors , we examined levels of Bcl 2 protein family members ( Bik / NBK , Bax , Bak , Bcl 2 , and Bcl XL ) , release of cytochrome c , and activation of caspase 9 and 3 in human colon cancer cell lines DLD 1 , LOVO , SW 620 , and HCT 116 ; human lung cancer cell line H 1299 ; and human ovarian cancer cell line SKOV 3 after they were treated with bortezomib . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , bcl 2 and bcl 10 change their expression in stromal cells , whereas bcl 10 was induced after drug treatment and bcl 2 down regulated in progenitor cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The adriamycin induced cell death and caspase 3 activation were significantly inhibited by SJH ( 2 mg / ml ) , which can be explained by the increase in Bcl 2 expression and the inhibition of Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , intracellular levels of the anti apoptotic proteins , such as Bcl 2 and Bcl xL , and the pro apoptotic protein Bax , were assessed during viral infection . ^^^ These analyses showed that as viral infection proceeded , the cellular level of Bcl xL decreased , while the levels of Bax and Bcl 2 remained unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mucosal expression of p 21 , p 27 , p 53 , Bcl 2 , and bax after small bowel resection and autotransplantation in pigs . ^^^ Eight weeks postoperatively , full thickness small bowel sections underwent routine immunohistochemistry for cell cycle inhibitors ( p 53 , p 21 , and p 27 ) , antiapoptotic Bcl 2 , and proapoptotic bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A number of proteins mediated with apoptotic process have been identified , including p 53 , BAX , BCL 2 and BCL 10 . ^^^ In the cultures exposed to pure anoxia , a significant increase of p 53 and BAX immunoreactivity , associated with the decreased level of BCL 2 and BCL 10 immunopositive cells was observed , related to the activation of apoptotic process . ^^^ Hippocampal cultures pretreated with ZnCl 2 before anoxia showed decreased immunoreactivity for p 53 and BAX , connected with BCL 2 overexpression , whereas the cultures exposed to zinc chelating agent TPEN or TPEN connected with anoxia showed significant increase of immunorectivity for p 53 and BAX . ^^^ This strong immunoreactivity of proapototic proteins ( p 53 and BAX ) in hippocampal cultures exposed to anoxia or / and TPEN correlated with previous ultrastructural evidences of anoxia and TPEN induced apoptosis , while the overexpression of anti apoptotic protein ( BCL 2 and BCL 10 ) in zinc pretreated cultures evidenced the protective ability of this metal against apoptosis in model of anoxia in vitro . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative evaluation of the apoptosis regulating genes Survivin , Bcl 2 and Bax in inflammatory and malignant pleural lesions . ^^^ MATERIALS AND METHODS : We quantified mRNA levels of the apoptosis regulating genes Survivin , member of the IAP family , Bcl 2 and Bax , members of the Bcl 2 family . ^^^ Bcl 2 and Bax levels were increased in inflammatory pleural samples ( 394 % , p=0 . 001 and 188 % , p=ns , respectively ) and in MMs ( 94 % , p=ns and 88 % , p=0 . 0163 , respectively ) . ^^^ The Bcl 2 / Bax ratio was higher in pleuritis than in MMs , compared to normal pleurae ( 441 % , p=ns and 22 % , p=ns , respectively ) ; the difference between Bcl 2 / Bax ratio in inflammatory and neoplastic pleural samples was significant ( p=0 . 00375 ) . ^^^ CONCLUSIONS : These results suggest that apoptotic defects in pleural MMs are linked to increased levels of Survivin , whereas variations in Bcl 2 and Bax expression appear less significant , although further studies are needed to highlight Bcl 2 family members interactions in apoptosis control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in apoptosis was associated with a dose dependent up regulation in pro apoptotic Bax expression , down regulation of anti apoptotic Bcl 2 , and proteolytic activation of caspase 3 protease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of apoptosis related genes such as BCL 2 , BAX , Fas receptor ( FAS ) , and Fas ligand ( FASLG ) was quantified using real time polymerase chain reaction ( PCR ) analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Enhanced sensitivity in combination with AS Bcl 2 was associated with down regulation of Bcl 2 and pAkt , which was correlated with the induction of Bax and caspase 3 , leading to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of Bax and Bcl 2 in penile carcinoma . ^^^ There is a complex interplay between the pro apoptotic Bax and anti apoptotic Bcl 2 family of proteins and the tumor suppressor gene p 53 . ^^^ The pathogenic role of Bax and Bcl 2 protein expression in penile carcinomas has not previously been investigated . ^^^ We examined Bax and Bcl 2 expression in verrucous ( VC ) and squamous cell carcinoma ( SCC ) of the penis . ^^^ Herein we also present a concise review of p 53 , Bcl 2 / Bax ratios , and their relationship to apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis of caspase 9 , Bax ( proapoptotic ) and Bcl 2 ( antiapoptotic ) proteins from the colon scraping suggests that quercetin and curcumin induce apoptosis via the mitochondrial pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After treatment , the following processes and factors were monitored : apoptosis , cellular metabolism and viability , expression of genes encoding HIF1A , von Hippel Lindau tumor suppressor protein ( VHL ) , and genes responsible for cell death induction and antiapoptotic defense ( P 53 , BCL 2 , BAX , and caspases 9 and 3 ) . ^^^ Hypoxia induced apoptosis by upregulating the P 53 , BAX , and caspase dependent cell death pathways , while activating cellular antiapoptotic defense by the overexpression of BCL 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transcriptional inactivation of p 53 , Bax , Bcl 2 and Mdm 2 correlates with malignant transformation of the uterine cervix . ^^^ Deregulation of the apoptotic machinery plays a major role in cell death , cellular transformation and cancer . p 53 , Bcl 2 , Bcl XL , Bax and Mdm 2 mRNA expression patterns were evaluated in tissue samples with cervical intraepithelial neoplasia ( CIN ) and cervical cancer compared to those of normal cervical tissues , and correlated with the underlying cervical lesions . ^^^ Transcript levels of the above genes were assessed by RT PCR analysis in a total of 44 cervical specimens . p 53 , Bcl 2 , Bax and Mdm 2 transcript levels were significantly different in the normal , CIN and cancer specimen groups ( p=0 . 003 , p=0 . 009 , p=0 . 040 and p=0 . 001 , respectively ) . ^^^ Specifically , p 53 , Bax and Bcl 2 exhibited substantially lower transcript levels in CIN lesions compared to controls , whereas Bax mRNA levels showed a significant decrease in cancer compared to normal specimens . ^^^ Coexpression analysis revealed more correlations between the above apoptosis related molecules in normal tissues compared to CIN or cancer specimens . p 53 showed significant coexpression with Bax , Bcl 2 and Mdm 2 ( p=0 . 040 , p=0 . 013 and p=0 . 015 , respectively ) in normal cervical specimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVES : The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl 2 , Bcl XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis . ^^^ The levels of Bcl 2 , Bcl XL , and Bax mRNA expression were analyzed by semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Semiquantitative RT PCR revealed variable mRNA expression , with the Bcl 2 / Bax ratio ranging from 0 . 2 to 1 . 5 and the Bcl XL / Bax ratio ranging from 0 . 3 to 1 . 8 . ^^^ Immunohistochemical analysis showed positive Bcl 2 , Bax , Bcl XL expression in 20 % , 72 % , and 92 % of cancer samples ; however , their levels were variable . ^^^ In contrast , Bcl 2 , Bax protein levels did not show any association with the apoptotic index . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The activity of caspase 3 was assessed using Western blotting and the expression of Bcl 2 and Bax genes was analyzed using the reverse transcription polymerase chain reaction ( RT PCR ) method . ^^^ Meanwhile , GC 51 markedly reduced the expression of the anti apoptotic gene Bcl 2 and increased the expression of the pro apoptotic gene Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , in a group of 10 patients and 10 healthy donors , it was also investigated by western blot if apoptosis and metastasis related proteins such as Bax , Bcl 2 and Hlm were present in serum . ^^^ Bax , Bcl 2 and Hlm were not detected in serum . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Particular attention is paid to the role of cytochrome c release from mitochondria and alterations in the pro and anti apoptotic proteins , Bax and Bcl 2 , respectively . ^^^ For immunocytochemistry , antibodies directed against activated caspase 3 , cytochrome c , Bcl 2 and Bax proteins were used . ^^^ RESULTS : Our results show that in hippocampi of aged baboons the immunoreactivities of the antiapoptotic agent Bcl 2 was not prominently changed , of the proapoptotic agent Bax was upregulated , of the cytochrome c was redistributed , and that caspase 3 was not activated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we used Bak / mouse liver mitochondria and virally immortalized Bax / Bak / mouse embryonic fibroblasts ( MEFs ) to investigate whether or not multidomain proapoptotic BCL 2 family proteins were required for arsenic induced mitochondrial damage and cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of U 937 cells with MNNG resulted in the inhibition of viability and the induction of apoptosis in a concentration dependent manner , which was associated with a dose dependent upregulation in pro apoptotic Bax protein , downregulation of anti apoptotic Bcl 2 and Bcl xL proteins , and proteolytic activation of caspase 3 protease . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prion protein ( PrP ) prevents Bcl 2 associated protein 10 ( Bax ) mediated cell death , but the step at which PrP inhibits is not known . ^^^ We first show that PrP is very specific for Bax and can not prevent Bak ( Bcl 2 antagonist killer 1 ) , tBid , staurosporine or thapsigargin mediated cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Structure activity studies have shown that the neuroprotective activity is associated with the propargyl moiety of rasagiline which protects mitochondrial viability and MPTp by activating Bcl 2 and protein kinase C ( PKC ) , and down regulating pro apoptotic FAS and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , it was found that pre treatment with melatonin inhibits Abeta induced increase in the levels of bax mRNA and that it enhances the level of bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ioversol , a radiocontrast medium , caused cell injury accompanied by decreases in Bcl 2 , increases in Bax , and caspase activation in LLC PK 1 cells . ^^^ In dominant negative CREB transfected cells , dibutyryl cAMP no longer prevented cell injury or inhibited changes in mRNA expression of Bcl 2 and Bax . ^^^ In mice with unilateral renal occlusion , ioversol increased urinary excretion of N acetyl beta d glucosaminidase with concomitant decreases in Bcl 2 mRNA , increases in Bax mRNA , activation of caspase 3 , and induction of apoptosis in tubular and interstitial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These differential effects correlated with the expression of the proapoptotic BCL 2 associated 10 protein ( BAX ) , which was downregulated in HUVECs and upregulated in the two neoplastic cell lines . ^^^ However , beta carotene induced expression changes of BAX and other BCL 2 pathway genes did not lead to the predicted induction of apoptosis in the A 375 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we used embryonic fibroblasts derived from mice deficient in the multidomain proapoptotic members of the Bcl 2 family ( Bax and Bak ) and the apoptotic components of the apoptosome ( Apaf 1 and caspase 9 ) to unravel the cascade of events by which Bcl 10 ( S ) promotes apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study investigates the expression of apoptotic proteins Bax , Bad , Bcl 2 , and Bcl xl following hypoxia in the cerebral cortex of the guinea pig fetus as a function of gestational age . ^^^ Hypoxia resulted in increased expression of the proapoptotic proteins Bax and Bad by 20 % and 30 % in the preterm as compared to 24 % and 38 % at term , without altering the expression of anti apoptotic proteins Bcl 2 and Bcl xl . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , m 3M3FBS induced apoptosis appeared to involve the down regulation of anti apoptotic Bcl 2 , the up regulation of pro apoptotic Bax , the release of cytochrome c , and caspase activation . m 3M3FBS induced apoptosis of U 937 cells was also partly inhibited by BAPTA AM and EGTA , indicating the involvement of intracellular calcium signaling on the apoptosis in U 937 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that doxorubicin did not significantly change the intracellular concentration of Bcl 2 , Bax and Bad in HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Involvement of Bax , Bcl 2 and caspase 3 in hydroxyurea or etoposide induced apoptosis of mouse interleukin 3 dependent lymphoma cells . ^^^ Chemical treatments or interleukin 3 withdrawal rendered level changes in Bax and Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression rate of p 53 , p 16 , C myc , Bcl 2 , and Bax proteins were studied by using immunohistochemical staining . ^^^ The expression of p 53 , p 16 , C myc , and Bcl 2 were significantly downregulated ( p < 0 . 01 ) , and the expression of Bax was upregulated over the time of coculture in the `` CIK ' ' experimental group , compared to the control group . ^^^ The CIK cells kill MGC 803 gastric cancer cells by inducing apoptosis in the early stage and by inducing necrosis in the late stage through the downregulating expression of p 53 , C myc , and Bcl 2 and the upregulating expression of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The role of p 53 and bax / bcl 2 in drug induced apoptosis was assessed in six prostate cell lines , 1532T , 1535T , 1542T , 1542N , BPH 1 and LNCaP using TD ( 50 ) concentrations of etoposide , vinblastine and estramustine . ^^^ Immunoblotting demonstrated that bax and bcl 2 proteins were expressed at a basal level in all cell lines , but did not increase after exposure to TD ( 50 ) doses of the three drugs . ^^^ The ratio of bax and bcl 2 , measured by laser scanning densitometry , was not altered by the drug induced DNA damage . ^^^ The results suggest that apoptosis is not a major mechanism of drug induced cell death in prostate cell lines and appears to be independent of p 53 status and bax / bcl 2 expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A variety of prognostic factors can predict outcome and include the karyotype of the leukemic cells and the presence of transmembrane transporter proteins , which extrude certain chemotherapy agents from the cell and confer multidrug resistance and mutations in or over expressions of specific genes such as WT 1 , CEBPA , BAX and the ratio of BCL 2 to BAX , BAALC , EVI 1 , KIT , and FLT 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The translocation of p 53 in MCF 7 cells results in a decrease in its transcriptional activity , as revealed by diminished p 21 ( WAF1 / CIP1 ) induction and an altered ratio of Bax and Bcl 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This cell growth inhibition induced apoptosis , which was associated with the down regulation of Bcl 2 , up regulation of Bax , depolarization of mitochondrial transmembrane potential ( Delta psi m ) , and caspase 3 and 9 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to understand the molecular alterations leading to heterogeneous cisplatin sensitivity and apoptosis inducibility in NSCLC cells , we analyzed various apoptotic pathways , including the activation of caspase 8 , 9 and 3 , the release of cytochrome c from mitochondria and the expression levels of pro and anti apoptotic proteins such as Bax , Bad , Bcl 2 , Bcl xL , Fas and p 53 using heterogeneously apoptosis sensitive cells ( Ma 10 , Ma 31 and Ma 46 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Telomerase activity was measured by TRAP enzyme linked immunosorbent assay , and the expression of Bcl 2 and Bax proteins was detected by western blot analysis . ^^^ Telomerase activity as well as Bcl 2 expression was down regulated , while Bax expression was up regulated concurrently , when apoptosis ocurred . ^^^ We therefore conclude that oridonin demonstrated anti proliferative and apoptosis inducing effects on K 562 cells in vitro , and that changes in bcl 2 and bax protein levels as well as telomerase activity may play an important role in its mechanism of action . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All lymphomas were CD 20 , CD79a , BAX positive , CD 3 , CD 30 , EMA , CD 10 , CD 5 , CD 59 , c myc , Bcl 2 , EBV ( LMP ) , CK negative . ^^^ Positive immunohistochemical results with BAX and the lack of Bcl 2 , c myc and CD 59 are associated with better prognosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CONCLUSION : Increased expression of Bax and Bak but not other Bcl 2 family members is associated with apoptosis in small intestinal crypts and may amplify the sensitivity and susceptibility of crypt cells to chemotherapy induced enteropathy . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis pathway has numerous targets for molecular therapeutic development , including p 53 , Bax , Bcl 2 , cFLIP , effector caspases , growth factor receptors , phosphatidylinositol 3 kinase , Akt and apoptosis inhibitors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These genes are from classes with different biological function ; for example , regulation of proliferation ( BUB 3 , cyclins , CDC25A ) , angiogenesis ( vascular endothelial growth factor and its receptor , matrix metalloproteinase 9 , angiostatin , thrombospondin 1 ) or apoptosis ( BCL 2 , BAX ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , inhibition of ERK through addition of PD 98059 stimulated p 53 activation in MCF 7 cells and also led to upregulation of p 53 downstream targets , p 21 and Bax , which is a proapototic member of Bcl 2 family triggering mitochondrial pore opening . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Sphingosine 1 phosphate is involved in cytoprotective actions of calcitriol in human fibroblasts and enhances the intracellular Bcl 2 / Bax rheostat . ^^^ As S1P has been identified to modify the Bcl 2 / Bax ratio in epithelial cells and keratinocytes , we also measured the expression of these proteins in dermal fibroblasts revealing an increased Bcl 2 level after stimulation with S1P while the Bax protein expression was not modified . ^^^ In conclusion , calcitriol H was revealed to protect human fibroblasts from apoptosis by formation of S1P resulting in a changed Bcl 2 / Bax ratio . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Between benign and borderline tumors , the P 21 and Bax expression levels were significantly higher in borderline tumors , whereas the Bcl 2 expression level was much higher in benign tumors ( P < 0 . 01 , each ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Nicergoline increased the basal levels of Bcl 2 and reduced the increase in Bax levels induced by beta amyloid , indicating that the drug inhibits the execution of an apoptotic program in cortical neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both caused apoptosis by increasing the protein expression of Bax and Bcl xs while decreasing Bcl 2 and Bcl 10 ( L ) , releasing apoptogenic cytochrome c , and augmenting the activity of caspase 9 and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Clinicopathological significance of Bcl 2 and Bax protein expression in human pancreatic cancer . ^^^ AIM : To assess the clinicopathological significance of the expression of the apoptosis inhibitory Bcl 2 protein ( pBcl 2 ) and the apoptosis promoting Bax protein ( pBax ) in human invasive ductal carcinomas ( IDCs ) of the pancreas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Microglial turnover was assessed using terminal deoxynucleotidyl transferase mediated dUTP nick end labelling ( TUNEL ) ( DNA damage ) , BAX ( proapoptotic marker ) , Fas ( CD 95 ) ( proapoptotic ) , proliferating cell nuclear antigen ( PCNA ) ( proliferation and DNA repair ) , Ki 67 ( cell proliferation ) and BCL 2 ( antiapoptosis ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , BAX , and apoptosis in endometrial hyperplasia after high dose gestagen therapy : a comparison of responses in patients treated with intrauterine levonorgestrel and systemic medroxyprogesterone . ^^^ METHOD : Pre and post treatment paraffin embedded endometrial hyperplasia specimens from women treated with levonorgestrel intrauterine device ( n = 26 ) and women treated with 10 mg medroxyprogesterone for 10 days per cycle ( n = 31 ) were examined for changes in the expression of Bcl 2 and BAX and the extent of apoptosis after 3 months of treatment . ^^^ Immunohistochemical expression in tissue specimens for Bcl 2 and BAX was evaluated by H score . ^^^ A second group of patients with endometrial hyperplasia was examined after 1 week treatment with levonorgestrel IUD ( n = 6 ) or medroxyprogesterone ( n = 5 ) to determine early effects on expression of Bcl 2 and BAX and the extent of apoptosis . ^^^ The decrease in BAX expression after IUD treatment was less than the reduction of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with the inhibitory effect on Abeta 25 35 induced stress induced cell death , sesaminol glucosides decreased expression of pro apoptotic gene p 53 , and Bax and caspase 3 , but enhanced expression of anti apoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , even if the proapoptotic protein Bax was dominantly expressed and Bcl 2 was down regulated , caspase 3 was not activated , DNA laddering was not detected , and number of positive TUNEL cells was not increased in liver of CBS deficient mice compared with wild type mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We hypothesize that Bcl 2 protein augmentation with adenovirus vector mediated bcl 2 ( Adv bcl 2 ) gene transfer may improve ischemia / reperfusion induced renal proximal and distal tubular apoptosis through the mitochondrial control of Bax and cytochrome C translocation . ^^^ Twenty four hours of Adv bcl 2 transfection to proximal and distal tubular cells in vitro upregulated Bcl 2 / Bax ratio and inhibited hypoxia / reoxygenation induced cytochrome C translocation , O ( 2 ) ( ) production and tubular apoptosis . ^^^ With a real time monitoring O ( 2 ) ( ) production and apoptosis analysis of rat kidneys , ischemia / reperfusion increased renal O ( 2 ) ( ) level , potentiated proapoptotic mechanisms , including decrease in Bcl 2 / Bax ratio , increases in caspase 3 expression and poly ( ADP ribose ) polymerase fragments and subsequent proximal and distal tubular apoptosis . ^^^ However , Adv bcl 2 administration significantly enhanced Bcl 2 / Bax ratio , decreased ischemia / reperfusion induced O ( 2 ) ( ) amount , inhibited proximal and distal tubular apoptosis and improved renal function . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Influence of prostaglandin A 2 and 2 methoxyestradiol on Bax and Bcl 2 expression levels in cervical carcinoma cells . ^^^ Bax can be regarded as pro apoptotic , whereas Bcl 2 is perceived as anti apoptotic . ^^^ It has been proposed that an increased ratio of pro apoptotic Bax to anti apoptotic Bcl 2 can be associated with apoptosis . ^^^ Since prostaglandin A 2 ( PGA 2 ) and 2 methoxyestradiol ( 2 ME ) play an active role in the induction of apoptosis , the influence of 20 microg / ml PGA 2 and 1 microM 2 ME was investigated on Bax and Bcl 2 expression levels in cervical carcinoma cells . ^^^ The Bax / Bcl 2 ratios for PGA 2 and 2 ME exposed cells were 2 . 06 and 1 . 87 respectively , normalised against Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To evaluate the use of various molecular markers as predictors of the response to CRT in rectal carcinoma , we investigated immunohistochemical expressions of p 53 , p 21 WAF1 / CIP1 , Bcl 2 , Bax , Ki 67 , Ku 70 , and 2 new candidate markers ( histone deacetylase 1 and metabotropic glutamate receptor 4 ) in pretreatment biopsy samples of 130 rectal carcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibition of core protein expression also altered the expression level of Bcl 2 family proteins , displaying an increase of the proapoptotic Bax and a decrease in the level of the anti apoptotic Bcl xL proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
On the other hand , no induction was observed of the pro apoptotic Bcl 2 family members Bax and Bid at any time from 3 to 24 h following PEDF addition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In these models , both SAHA and m carboxycinnamic acid bis hydroxamide induced growth arrest and caspase mediated apoptosis and increased p 21 protein levels , retinoblastoma hypophosphorylation , BH 3 interacting domain death agonist cleavage , Bax up regulation , down regulation of Bcl 2 , A 1 , and Bcl 10 ( L ) expression , and cleavage of poly ( ADP ribose ) polymerase and caspase 8 , 9 , 3 , 7 , and 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of bcl 2 and bax protein in uterine leiomyosarcomas and leiomyomas ] . ^^^ OBJECTIVE : To determine the expression and significance of the apoptosis regulatory protein bcl 2 and bax in uterine leiomyosarcomas and uterine leiomyomas . ^^^ METHODS : The expression of bcl 2 and bax were examined by immunohistochemical ABC staining with specific antibodies in 34 cases of uterine leiomyosarcomas ( 18 cases in stage 1 , 1 in stage 2 , 8 in stage 3 , and 7 in stage 4 ) , 34 uterine leiomyomas and 34 normal myometrium samples . ^^^ Both the expressions of bcl 2 and bax proteins in stage 1 2 were higher than those of in stage 3 4 ( P < 0 . 01 ) . ^^^ CONCLUSION : The imbalance between the expression of bcl 2 and bax may play a certain role in the genesis of the uterine leiomyomas and leiomyosarcomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Several connections were demonstrated between Cox and a few oncogenes ( 5 src , 5 Ha ras , HER 2 \ neu , Wnt , p 53 mutated ) , alimentary products ( PUFAs ) , transcription factors ( c jun and c fos ) , proapoptotic proteins [ Bax et Bcl 10 ( L ) ] or antiapoptotic ( Bcl 2 ) , CYP 19 aromatase gene , NFkappaB receptor ( RANKL ) , angiogenesis ( via VEGF , TXA 2 , oxid nitric synthetase , alphaVbeta 3 integrin receptor ) , peroxisome gamma proliferator receptor ( PPARgamma ) and its ligand PGJ 2 and with antitubuline chemotherapy drugs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The Bim accumulation in mitochondria enhances interaction of Bim with Bcl 2 , relieves Bax from Bcl 2 bound complexes , and subsequently results in release of cytochrome c into the cytoplasm . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of HepG 2 cells with 6ME resulted in the release of mitochondrial cytochrome c followed by the activation of caspase proteases , and subsequent proteolytic cleavage of poly ( ADP ribose ) polymerase . 6ME increased the expression of p 53 and bax and decreased the expression of bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This work describes the isolation of cDNA and genomic fragments from five sheep BCL 2 related genes : BCL 2 , BCL2L1 , BCL2L2 , BAX and MCL 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , TQ treatment of HL 60 cells caused a marked increase in Bax / Bcl2 ratios due to upregulation of Bax and downregulation of Bcl 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Maspin expressing tumor cells have a reduced level of anti apoptotic protein Bcl 2 , and an increased level of pro apoptotic protein Bax . ^^^ The regulation is not controlled at the transcriptional level but is through selective control of Bcl 2 and Bax protein stability . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , PK 11195 elicited apoptosis was associated with a downregulation of Bcl 2 , translocation of Bax to the mitochondria including subsequent oligomerization , and activation of caspase 9 , indicating the involvement of mitochondria in PK 11195 induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neither antioxidant treatments nor apoptotic inducers alone altered expressions of Bcl 2 , Bax , HSP 70 or pERK1 / 2 or pJNK . alpha lipoic decreased pERK 2 in staurosporine treated cells in a dose dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NPD 1 also up regulates the anti apoptotic Bcl 2 proteins Bcl 2 and BclxL and decreases pro apoptotic Bax and Bad expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax protein interaction in B lymphocytes of peripheral blood in patients with chronic lymphocytic leukemia ] . ^^^ Evidence indicates that Bcl 2 and Bax form homo and heterodimers . ^^^ The anti apoptotic effect of Bcl 2 protein is based on its ability to bind Bax protein in the heterodimer form , and thus to block the forming of Bax / Bax proapoptotic homodimers . ^^^ The ratio of Bcl 2 / Bax represents the cell autonomous rheostat which determinates the type of the cell reaction to an apoptotic stimulus . ^^^ Specimens were precipitated with the monoclonal antibody for Bcl 2 protein , and immunoblotted with the palyclonal antibady for Bax protein ( IP : Bcl 2 / WB : Bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It is believed that oesophageal cancer has an intricate molecular mechanism of evading apoptosis by the down regulation of Bax , up regulation of Bcl 2 , Bcl xl and Survivin , mutation of p 53 and alteration in Fas expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MMP 9 , MMP 2 , tissue inhibitor of MMP ( TIMP ) 1 , TIMP 2 , Bax , and BCl 2 proteins in retinal extracts were determined by Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Semi quantitative reverse transcription PCR ( RT PCR ) was used to analyze the expression of bcl 2 and bax genes . ^^^ Picroside 2 10 mg / kg was found to protect hepatocytes against apoptosis in a dose dependent manner ; it up regulated the expression of bcl 2 genes , thus increased the bcl 2 / bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , high doses of TZD induced massive apoptosis in renal cancer cells , with increased Bax expression and decreased Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIMS : To examine the prognostic relevance of the expression of the Bcl 2 , Bcl xL , and Bax proteins in stage IB squamous cervical carcinoma ( SCC ) . ^^^ Immunohistochemistry using monoclonal antibodies against Bcl 2 , Bcl xL , and Bax was used to examine protein expression . ^^^ RESULTS : Cytoplasmic expression of Bcl 2 , Bcl xL , and Bax was low ( < 5 % positive cells ) in 159 of 220 ( 73 % ) , 193 of 220 ( 87 % ) , and 39 of 220 ( 18 % ) tumours , respectively , and high ( > or = 5 % positive cells ) in 61 of 220 ( 27 % ) , 27 of 220 ( 13 % ) , and 181 of 220 ( 82 % ) tumours , respectively . ^^^ CONCLUSION : Bcl 2 , Bcl xL , and Bax were not independently associated with prognosis in stage IB SCC . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The presence of trastuzumab markedly attenuated the relative increase on p 27 expression and the Bax : Bcl 2 ratio induced by gefitinib . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 protein levels were greater in suspended muscles relative to control muscles in both age groups . ^^^ Our data also show that aging favors the proapoptotic signaling in skeletal muscle by altering the contents of Bax , Bcl 2 , Apaf 1 , AIF , caspases , XIAP , Smac / DIABLO , and cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Propofol dose dependently reduces tumor necrosis factor alpha Induced human umbilical vein endothelial cell apoptosis : effects on Bcl 2 and Bax expression and nitric oxide generation . ^^^ The antiapoptotic Bcl 2 and proapoptotic Bax protein expressions were measured by immunocytochemical analysis . ^^^ TNF stimulation resulted in a reduced Bcl 2 / Bax ratio and increased apoptotic index ( AI : percentage of apoptotic cells ) in HUVECs . ^^^ Propofol , at concentrations > / =12 muM , significantly ( P < 0 . 001 ) and dose dependently attenuated TNF induced increase in AI and decrease in Bcl 2 / Bax ratio . ^^^ There is an inverse correlation between the ratio of Bcl 2 / Bax expression and AI ( P = 0 . 0009 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To investigate the effect of an antioxidant edaravone on the apoptotic process , we examined Bax and Bcl 2 immunohistochemical expression and terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling ( TUNEL ) reactivity . ^^^ After 24 h of reperfusion , infarction volume assessments , Bax and Bcl 2 immunohistochemistry and TUNEL staining were performed as well as neurological evaluation . ^^^ In addition , edaravone treatment provided a significant reduction in the number of TUNEL positive apoptotic cells , a decrease in Bax immunoreactivity and an increase in Bcl 2 expression within the peri infarct area . ^^^ Edaravone shows an excellent neuroprotective effect against ischemia / reperfusion brain injury through a Bax / Bcl 2 dependent anti apoptotic mechanism . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered expression of p 53 , Bcl 2 and Bax induced by microcystin LR in vivo and in vitro . ^^^ In spite of extensive studies on MC LR mediated cell damages , there is little information on the protein expression of p 53 and Bcl 2 , Bax in vivo and in vitro , which are vital regulator of apoptosis in response to a variety of stimuli . ^^^ The present study was undertaken to determine the expression level of p 53 and Bcl 2 , Bax in cultured hepatocytes and rat liver tissues . ^^^ The results show that MC LR can increase the expression of p 53 and Bax significantly both in vivo and in vitro , however , MC LR can only decrease the expression of Bcl 2 significantly in vitro and there is no difference observed in vivo . ^^^ It can be concluded that the expression of p 53 , Bcl 2 and Bax are involved in the regulation of MC LR induced apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , BV induces apoptosis in rheumatoid synovial cells through a decrease in BCL 2 expression and an increase in BAX and caspase 3 ( CASP 3 ) expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the relationships between changes in the carcinoma cell proliferation index , apoptotic index ( AI ) , and apoptosis related factors , including bcl 2 and bax , after different durations of neoadjuvant hormonal therapy ( NHT ) in prostate cancer tissue . ^^^ Immunohistochemistry was used to determine the expression of bcl 2 and bax and to determine the proliferation index , and the terminal deoxynucleotidyl transferase mediated nick end labeling method was used to assess apoptosis and determine the AI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The molecular mechanism of estrogenic neuroprotection does not appear to involve modulation of the antiapoptotic Bcl 2 or the proapoptotic Bax since we failed to observe changes in the levels of the two proteins in the neocortical tissue after gp 120 and / or E 2 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Di ( 2 ethylhexyl ) phthalate ( DEHP ) increases Bcl 2 / Bax ratio and modifies c myc expression in Syrian hamster embryo ( SHE ) cells . ^^^ The RNA expression levels of the regulator genes involved in the apoptotic pathway , bcl 2 , bax and of c myc were measured using Western blotting and RT PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present review we discuss the role of the most extensively examined molecules involved in the apoptotic procedure , such as bcl 2 , bax , fas / fasL , survivin and p 53 , as well as the incidence of baseline apoptosis in various brain tumors , in relation to prognosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Prenatal auditory enrichment with species specific calls and sitar music modulates expression of Bcl 2 and Bax to alter programmed cell death in developing chick auditory nuclei . ^^^ On the other hand , Bax immunohistochemistry showed correlated reverse changes compared to Bcl 2 expression . ^^^ Thus prenatal extra acoustic stimulation appears to alter Bcl 2 and Bax expression to support cell survival and differentiation , thereby augmenting the development of auditory nuclei . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunohistochemistry the groups were compared for expression of apoptotic proteins : bcl 2 , bcl 10 ( L ) , bax , bak and survivin . ^^^ Radioresistant laryngeal cancer was associated with bcl 2 ( P < 0 . 001 ) and bcl 10 ( L ) ( P = 0 . 005 ) expression and loss of bax expression ( P = 0 . 012 ) in pretreatment biopsies . ^^^ The association between expression of bcl 2 , bcl 10 ( L ) and bax with radioresistant cancer suggests a potential mechanism by which cancer cells avoid the destructive effects of radiotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the genetic expression of bcl 2 and bax , both of which have been implicated in apoptosis , was regulated by treatment with Rb 1 and Rg 1 , at a concentration of 250 microg / mL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the apoptosis regulating genes , BCL 2 expression was diminished out , whereas BAX and CASP 3 expressions were increased , compared with control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fibroblast growth factor 2 applied to the optic nerve after axotomy increases Bcl 2 and decreases Bax in ganglion cells by activating the extracellular signal regulated kinase signaling pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present studies showed that MAP could induce cell apoptosis which was closely accompanied with an increase of intracellular free calcium concentration ( [ Ca2+ ] 1 ) , the enhancement of reactive oxygen species ( ROS ) level , dissipation of mitochondria membrane potential ( MMP ) , up regulation of p 53 mRNA , increase expression of Bax mRNA , and decrease expression of Bcl 2 mRNA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In parallel experiments , the expression of genes related to apoptosis , BCL 2 , BCLXL , BAX , P 53 , APAF 1 , Caspase 3 , and Caspase 9 , was determined by RT PCR . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of P 53 , Bcl 2 and Bax proteins in rat liver after exposed to microcystin LR . ^^^ The expression of P 53 , Bax and Bcl 2 in liver was detected by Western blot . ^^^ Moreover , with exposure levels increasing the expression of P 53 and Bax increased gradually ; while no changes of the expression of Bcl 2 were observed . ^^^ CONCLUSION : P 53 and Bax may play important roles in microcystin LR induced apoptosis , but Bcl 2 seems not be involved in this process . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study examined the effects of progesterone on mRNA and protein levels of the Bcl 2 apoptosis regulatory genes , bax , bad , bcl 2 , and bcl 10 ( L ) , in cerebral cortex after TBI . ^^^ Our results indicate that bax and bad mRNA levels and Bax and Bad protein expression in the ipsilateral , injured cerebral cortex were significantly elevated post TBI , while mRNA levels of bcl 2 and bcl 10 ( L ) or Bcl 2 and Bcl 10 ( L ) protein expression were not changed . ^^^ Under the sham treated condition , progesterone significantly increased mRNA levels of the anti apoptotic gene , bcl 2 , but down regulated pro apoptotic gene expression ( bax and bad ) in cerebral cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These events were accompanied by the inactivation of ERK and AKT , generation of reactive oxygen species ( ROS ) , disruption of mitochondrial membrane potential ( ( Delta ) psi ( m ) ) , decrease of mitochondrial Bcl 2 , and increase of mitochondrial Bax content . ^^^ Furthermore , the free radical scavenger ascorbic acid and N acetylcysteine attenuated emodin mediated ROS production , ERK and AKT inactivation , mitochondrial dysfunction , Bcl 2 / Bax modulation , and apoptosis . ^^^ Take together , these findings suggest that in A 549 cells , emodin mediated oxidative injury acts as an early and upstream change in the cell death cascade to antagonize cytoprotective ERK and AKT signaling , triggers mitochondrial dysfunction , Bcl 2 and Bax modulation , mitochondrial cytochrome c release , caspase activation , and consequent leading to apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of vascular endothelial growth factor ( VEGF ) and the apoptotic markers Bcl 2 and Bax was analyzed by Western blotting . ^^^ The relative ratio of Bcl 2 to Bax proteins was decreased by approximately 90 % , indicating a net apoptotic gain and efficacy of treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TAp63alpha upregulates expression of proapoptotic Bcl 2 family members like Bax and BCL2L11 and the expression of RAD 9 , DAP 3 and APAF 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of insulin like growth factor 1 receptor , estrogen receptor alpha , Bcl 2 and Bax proteins in human breast cancer . ^^^ Consequently , we examined by immunohistochemistry the expression of the insulin like growth factor 1 receptor ( IGF IR ) , estrogen receptor alpha ( ERalpha ) and apoptosis associated proteins , Bcl 2 and Bax , in human primary breast cancer , as well as analyzing the relationships among these proteins . ^^^ The positive immunostaining for IGF IR , ERalpha , Bcl 2 and Bax was noted in 56 , 63 . 8 , 82 . 8 and 50 % of tumors , respectively . ^^^ Expression of ERalpha correlated positively with Bcl 2 and negatively with Bax proteins ( p < 0 . 0001 , p < 0 . 05 , respectively ) . ^^^ We did not note significant relationships between IGF IR and Bcl 2 , or IGF IR and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The level of Bcl 2 was slightly decreased , in contrast , the level of Bax elevated markedly , which resulted in a significant decrease of Bcl 2 / Bax ratio after PA treatment on HepG 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also showed that GD 3 , the voltage dependent anion channel 1 ( VDAC 1 ) and the fission protein hFis 1 are structural components of a multimolecular signaling complex , in which Bcl 2 family proteins ( t Bid and Bax ) are recruited . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Examined apoptosis related genes included tumor suppressor genes p 53 , p 53 ; mouse double minute 2 , MDM 2 ; cyclin dependent kinase inhibitor p 21 ( WAF 1 ) , p 21 ( waf ) ; Bcl 2 associated protein 10 , BAX ; apoptotic protease activating factor 1 , Apaf 1 ; Caspase 9 and serine / threonine protein kinase , PKB . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Intravenous acid fibroblast growth factor protects intestinal mucosal cells against ischemia reperfusion injury via regulating Bcl 2 / Bax expression . ^^^ AIM : To detect the effect of acid fibroblast growth factor ( aFGF ) on apoptosis and gene expression of bax and bcl 2 gene in rat intestine after ischemia / reperfusion ( I / R ) injury , and to explore the protective mechanisms of aFGF . ^^^ Intestinal tissue samples were taken not only for detection of bax and bcl 2 gene expression by RT PCR , but also for detection of bax and bcl 2 protein expression and distribution by immunohistochemical analysis . ^^^ The bax gene transcription and translation were significantly decreased in group A vs group R , while mRNA and protein contents of Bcl 2 in group A were obviously higher than those in group R during 2 12 h period after reperfusion . ^^^ CONCLUSION : The changes in histological structure and the increment of apoptotic rate indicated that the intestinal barrier was damaged after intestinal I / R injury , whilst intravenous aFGF could alleviate apoptosis induced by ischemia and reperfusion in rat intestinal tissues , in which genes of bax and bcl 2 might play important roles . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax are important molecules involved in the regulation of apoptosis . ^^^ METHODS : Paraffin embedded tissues from 50 patients of prostate carcinoma were examined for the expression of bcl 2 antiapoptotic and bax proapoptotic markers and also proliferation marker , Ki 67 , by immunohistochemistry . ^^^ Correlation between apoptotic index , proliferation index and bcl 2 and bax expression with stage , pathological grade and Gleason score was determined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase of caspase 8 , 9 , 3 activities revealed that caspase was a key mediator of the apoptotic pathway induced by grifolin , and the underexpression of Bcl 2 and up regulation of Bax resulted in the increase of Bax : Bcl 2 ratio , suggesting that Bcl 2 family involved in the control of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These genes are involved in cell cycle control ( e . g . p 21 , p 27 , CDK 2 , and cyclins ) , apoptosis regulation ( e . g . bcl 2 and bax ) , and signal transduction ( e . g . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of cell survival / death genes : Bcl 2 and Bax at the rate of colon cancer prognosis . ^^^ Apoptosis inhibition , in turn , depends partly on the balance between expression of two cell death regulatory genes , Bcl 2 and Bax . ^^^ The aim of our study was to determine the expression levels of Bcl 2 and Bax mRNAs in 1 microg sample of total RNA obtained from normal colon and colon adenocarcinoma . ^^^ This study was intended to evaluate possible differences in Bcl 2 and Bax mRNA levels at particular stages of colon adenocarcinoma classified according to Duke ' s system . ^^^ The apoptotic frequency ( represented by Bax mRNA copy number ) was inversely proportional to the decrease of Bcl 2 gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effects of hormone therapy , estrogen therapy and tibolone on markers of apoptosis including bcl 2 , and bax and cyclin D ( 1 ) expression in postmenopausal vaginal epithelium . ^^^ Bcl 2 , bax , Bcl 2 / bax ratio , cyclin D ( 1 ) measurements were performed immunohistochemically . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate whether proapoptotic Bcl 2 members , Bax and Bak , are involved in fetal vasculature regression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These protective effects are related to its ability to attenuate oxidative stress , regulate the expression of apoptotic proteins Bcl 2 , Bax , P 53 and caspase 3 , protect mitochondria , and interfere with APP metabolism . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Transcript levels of the genes encoding leptin receptor ( LEPR ) , signal transducer and activator of transcription ( STAT 3 ) , BCL 2 associated 10 protein ( BAX ) , and baculoviral inhibitor of apoptosis protein repeat containing 4 ( BIRC 4 , also known as XIAP ) , were determined by reverse transcriptase quantitative polymerase chain reaction analysis of expanded and hatched blastocysts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 / Bax gene expression in different types of carotid plaque ] . ^^^ OBJECTIVE : To investigate Bcl 2 / Bax gene expression in different types of carotid plaque , and examine the relationship between gene expression and atherosclerotic plaque instability and the main cause of brain ischemic events . ^^^ Bcl 2 / Bax was detected by immunohistochemistry ( IHC ) staining ( n=42 ) and in situ hybridization ( ISH ) ( n=25 , stable 13 / unstable 12 ) . ^^^ CONCLUSION : The expression rate of Bcl 2 / Bax in unstable plaques was higher than in stable plaques . ^^^ Bcl 2 was one of the elements that maintain plaque stability whereas Bax was one element that facilitates plaque instability . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Presently , studies of Bax mediated cardiac cell death examined primarily the expression levels of Bax and its prosurvival factor Bcl 2 rather than the localization of this protein , which dictates its function . ^^^ Also , we found that Bax translocation to mitochondria was associated with the exposure of an NH 2 terminal epitope , and that this translocation could be partially blocked by the prosurvival factors Bcl 2 and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of coumarin and 7OH coumarin on bcl 2 and Bax expression in two human lung cancer cell lines in vitro . ^^^ The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl 2 and Bax oncoproteins in two human lung cancer cell lines , A 427 and Calu 1 . ^^^ Total protein was extracted from cell lysates and the bcl 2 and Bax oncoproteins were identified . ^^^ Western blotting showed a decrease in bcl 2 and an increase in Bax in A 427 cells cultured with coumarin or 7 OHC . ^^^ Neither drug changed bcl 2 expression in Calu 1 cells compared to solvent controls , and Bax expression was only slightly increased by coumarin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It down regulated the Bcl 2 and Mcl 1 expression , and up regulated Bax protein in a time response manner . ^^^ These results conclude that Cin triggers apoptosis in PLC / PRF / 5 cells could be through the activation of pro apoptotic Bcl 2 family ( Bax and Bid ) proteins and MAPK signaling pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of dioxin responsive gene cytochrome P 450 1A1 ( CYP1A1 ) , apoptotic gene Bax , and anti apoptotic genes Bcl 2 and Bcl xL were examined in rat liver and brains using Western blot analysis and RT PCR . ^^^ These results indicate that early exposure of dioxin could affect the development of certain brain regions with gender difference , in terms of its differential effect on expressions of Bcl xL , Bcl 2 , and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These responses were associated with prominent changes in expression of several proteins that regulate apoptosis ( Bcl 2 , Bax , Apaf 1 , Caspase 9 cleavage , PARP cleavage ) and cellular growth , differentiation and stress response ( p 53 , Hsp 27 , NF kappaB ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses showed an increase in Bax : Bcl 2 ratio , release of cytochrome c from mitochondria , and calpain and caspase 3 activities during apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To further determine the molecular mechanism of apoptosis induced by GA , the changes on the expression of bcl 2 and bax genes were detected by RT PCR . ^^^ This effect connects with the inducing apoptosis in BGC 823 cells and the molecular mechanism might be related to the reduction of expression of apoptosis regulated gene bcl 2 , and the improvement of the expression of apoptosis regulated gene bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , smoke treatment produced an increase in the level of proapoptotic proteins ( Bax , t Bid , cytochrome c and caspase 3 ) , but a decline in Bcl 2 , procaspase 8 and procaspase 9 proteins . ^^^ Thus , CS induced apoptosis may result from two main mechanisms , one is the activation of p38 / JNK Jun FasL signaling , and the other is stimulated by the stabilization of p 53 , increase in the ratio of Bax / Bcl 2 , release of cytochrome c ; thus , leading to activation of caspase cascade . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax protein content was diminished in the loaded muscle relative to the control side from all groups , whereas Bcl 2 protein content was reduced in the young and aged muscles after 21 days of loading . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of bcl 2 , bcl xL , bax , p 53 proteins in gastric adenoma and adenocarcinoma ] . ^^^ BACKGROUND / AIMS : The aim of this study was to investigate the immunohistochemical expression of bcl 2 , bcl xL , bax , and p 53 proteins according to the pathological parameters such as grade of dysplasia , histological type , depth of invasion , lymph node metastasis , and TNM stage in the gastric adenoma and gastric adenocarcinoma . ^^^ METHODS : Immunohistochemical staining using monoclonal bcl 2 , bcl xL , bax , p 53 antibodies were performed on paraffin embedded specimens from forty one gastric adenomas and 100 gastric adenocarcinomas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic Bcl 2 , proapoptotic Bax and Bid , Smac , and cytochrome c oxidase ( COX 4 ) were analyzed by Western blot analyses from cytosol and mitochondrial fractions . ^^^ ASA downregulated Bcl 2 protein expression and induced Bax translocation into the mitochondria and cleavage of Bid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have previously demonstrated that ethanol induced neuron apoptosis is critically dependent on expression of Bax , a proapoptotic member of the Bcl 2 family . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increased phosphorylation of phosphatase and tensin homolog deleted from chromosome 10 ( PTEN ) and Bax protein , and decreased Akt / cyclic AMP response element binding protein ( CREB ) phosphorylation , including Bcl 2 protein in the vehicle treated group were not affected by treatment with aspirin , clopidogrel and MCI 186 , whereas those effects were reversed by cilostazol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot results illustrated that in the same dosage and incubation time , DMC could down regulate the level of Bcl 2 protein and did not influence the expression of Bax protein . ^^^ The resulting net effect could thus lead to a lower ratio of Bcl 2 / Bax , which might be responsible for the DMC induced apoptosis in K 562 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
IL 6 , its receptors and its relationship with bcl 2 and bax proteins in infiltrating and in situ human breast carcinoma . ^^^ AIMS : To characterize the expression pattern of IL 6 and its receptors ( IL 6R ( alpha ) and gp 130 ) , to relate this pattern to bcl 2 and bax expression and to elucidate the effects on the proliferation / apoptosis equilibrium in benign conditions and in situ and infiltrating breast cancer . ^^^ METHODS AND RESULTS : The immunoexpression of IL 6 and its receptors ( IL 6R ( alpha ) and gp 130 ) , and their relationship with bcl 2 and bax proteins , were studied in in situ and infiltrating tumours and in benign breast lesions by means of Western blotting and immunohistochemistry . ^^^ The percentages of samples positive for IL 6 , bcl 2 and bax and their immunoreaction densities were higher in in situ carcinomas and infiltrating tumours than in benign lesions ; although in in situ lesions were not so high as in infiltrating tumours , except for bax , whose immunoexpression was as weak as in benign conditions , resulting in a bcl 2 / bax ratio higher than in infiltrating tumours . ^^^ The increased bax immunoreactivity observed only in infiltrating tumours , which was not so high as the increase in bcl 2 immunoreactivity , might be interpreted as an attempt to hinder cell proliferation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regulation of apoptosis in granulosa cells was examined by annexin 5 and propidium iodide staining ; measurement of relative levels of mRNA encoding Bcl 2 , Bcl xL and Bax ; and activity of caspase 3 , 8 and 9 . ^^^ The ratios of relative levels of mRNA encoding Bcl 2 to Bax and Bcl xL to Bax were higher on day 6 than days 4 and 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of caspase 3 activity , bcl 2 bax and p 65 gene expression modulation in human acute promyelocytic leukemia HL 60 cells by doxorubicin with amifostine . ^^^ The influence of amifostine alone and in combination with doxorubicin , cytarabine , and etoposide on the cell growth and on bcl 2 , bax and p 65 gene expression was investigated in human acute promyelocytic leukemia cell line HL 60 . ^^^ Furthermore , the expression of bcl 2 and bax genes involved in apoptosis as well as tumor associated p 65 gene were determined . ^^^ Semiquantitative reverse transcriptase polymerase chain reaction showed a decrease in bcl 2 and an increase in bax and p 65 expression in HL 60 cells treated with doxorubicin in combination with amifostine when compared with the cells treated only with doxorubicin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : NOS isoenzymes , pro ( Bax ) and antiapoptotic factors ( Bcl 2 , Bcl XL ) , and neurotrophic factors ( brain derived neurotrophic factor , BDNF ; neurotrophin 3 , NT 3 ; fibroblast growth factor 2 , FGF 2 ) were determined by immunoblotting in the EL mouse brain at various developmental stages . ^^^ In DDY mice , very low steady state levels of Bcl 2 and Bax remained constant throughout development . ^^^ In EL mice , these Bcl 2 and Bax levels were increased even before experiencing frequent seizures . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proteins for Western blot analysis included Fas associated death domain ( FADD ) and caspase 8 for extrinsic pathway , as well as Bcl 2 , Bcl XL , Bax , Bad , Bid , Akt , p Akt , and caspase 9 for intrinsic pathway . ^^^ Levels of FADD , Bax , Bad , and Bid were substantially increased in the TA induced myopathy group , whereas Bcl 2 , Bcl XL , Akt , p Akt , and caspase 9 did not change between control and myopathy groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , expression of markers of apoptosis Bcl 2 , Bax , and cleaved caspase 3 was only slightly increased in iARF rats , compared to sham operated animals , and was also not influenced by L Arg . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic activity of BMP 7 in the LNCaP and C 4 2B cell lines was not associated with a significant alteration in the levels of the proapoptotic protein Bax or the antiapoptotic proteins Bcl 2 , Bcl xl , and 10 linked inhibitor of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further studies were conducted to compare these changes to the localization and expression of the bcl 2 group of proteins bcl 10 , bax and mcl 1 , and Fas Fas ligand in the same cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fraxetin at 10 100 muM inhibited the formation of ROS , cytochrome c release , activation of caspase 3 and 9 , and suppressed the up regulation of Bax , whereas no significant change occurred in Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was preceded by decrease in Bcl 2 protein level accompanied by caspase 9 activation and poly ( ADP ribose ) polymerase ( PARP ) cleavage without altering Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The survival function of IL 7 is largely through maintaining a favorable balance of bcl 2 family members including Bcl 2 itself and Mcl 1 on the positive side , and Bax , Bad and Bim on the negative side . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results showed that MEM induced cell apoptosis involved up regulation of Fas and down regulation of Bcl 2 , DR 3 , DR 4 , TNFRI , and TNFRII in HepG 2 cells , while no changes on the levels of Bax , Bid , Bad , and Bak protein were observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of anti apoptotic protein , Bcl 2 and pro apoptotic protein , Bax was visualized by Western blot analysis . ^^^ Greater Bcl 2 and attenuated Bax expression was found in TFEB treating rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we evaluate the expression of apoptosis related proteins of the Bcl 2 family ( Bcl 2 , Bax and Bcl xL ) in the traumatic penumbra area in correlation with the extent of apoptosis in the rat model of focal cerebral contusion , treated by HBO . ^^^ The pathological study was based on immunohistochemical staining of the brain sections for Bcl 2 , Bax and Bcl xL with quantitative evaluation of staining by image analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cultured Nb cells were sensitized to alpha TOS by pre treatment with Bcl 2 , Bcl xL or Mcl 1 siRNAs , while the malignant cell line was more resistant to the vitamin E analogue when Bax was knocked down . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MnTMPyP pre treated cells showed significant inhibition of apoptotic features such as activation of caspase 3 , up regulation of Bax and p 53 , and down regulation of Bcl 2 compared to control cells upon exposure to ionizing radiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In comparing model simulations with experimental results , we find the best agreement when Bcl 2 blocks the release of cytochrome c by binding to both Bax and truncated Bid instead of Bax , truncated Bid , or Bid alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects of AN 215 on apoptosis and cell proliferation were followed by histology , and the expression of Bcl 2 and Bax protein was determined by Western blot analysis . ^^^ Therapy with AN 215 , but not with AN 201 , decreased the ratio of Bcl 2 / Bax in DU 145 and the expression of antiapoptotic Bcl 2 in LuCaP 35 tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proapoptotic BH 3 only protein natural born killer / Bcl 2 interacting killer ( Nbk / Bik ) has been described to inhibit Bcl 2 and Bcl xL , thereby supporting the death promoting ability of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic cell death was largely dependent on the presence of the tumour suppressor p 53 , but did not require the Bcl 2 family member Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The morphological changes of the H 22 cells were observed by transmission electron microscope and the apoptosis related proteins Bcl 2 and Bax were examined by streptavidin biotin peroxidase complex ( SABC ) method . ^^^ In culture media with FZYLG medicated sera , the expression of Bcl 2 decreased while that of Bax increased as compared with that in culture medium with non medicated serum ( P < 0 . 05 ) . ^^^ CONCLUSION : FZYLG medicated serum can induce apoptosis of the liver cancer cells H 22 by influencing the cell cycle , down regulating the expression of Bcl 2 and up regulating the expression of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Upregulation of Bcl 2 is associated with cisplatin resistance via inhibition of Bax translocation in human bladder cancer cells . ^^^ In contrast , overexpressed Bcl 2 protein inhibited cisplatin induced Bax translocation and its downstream events in T24R2 . ^^^ Downregulation of Bcl 2 by RNAi potentiated the redistribution of Bax and cytochrome c and reversed cisplatin resistance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of Mcl 1 and Bcl XL but not those of Bcl 2 , Bax , and Bak were suppressed by TSA or SK 7041 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We evaluated the levels of Bax , Bcl xL , Bcl 2 and cytochrome c expression by Western blot analysis , and caspase 9 and 3 activities were determined in a colorimetric assay . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of A 549 cells by TSA was associated with a down regulation of anti apoptotic Bcl 2 protein and an up regulation of pro apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After exposure to the compound , the modulated genes were involved in inflammatory responses as with the mitogen activated protein kinase 14 ( MPK 14 ) , or in tumor and metastasis progression as with the matrix metalloproteinase 17 ( MMP 17 ) , in cell proliferation as with c jun and c fos , and moreover in the apoptotic process as with interferon alpha inducible protein ( IFI ) , BAX and BCL 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Female rats of 50 days old were injected with MNU ( 60 mg 10 kg ( 1 ) ) intraperitoneally , and three doses of nm haFGF ( 1 . 25 microg , 2 . 5 microg and 5 microg in one eye of each rat ) were injected , separately , into vitreous body of one eye of each rat twice a day at 0 and 12 h after MNU treatment . 24 h later , apoptotic index of photoreceptor cells was detected by TUNEL labeling and the expressions of Bcl 2 and Bax were analyzed by Western blotting . ^^^ The expressions of Bcl 2 increased and that of Bax decreased adversely after being injected with different doses of nm haFGF . ^^^ The mechanism could be related to up regulation of Bcl 2 and down regulation of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax / Bcl 2 expression levels of 2 methoxyestradiol exposed esophageal cancer cells . 2 Methoxyestradiol ( 2ME ) , an endogenous metabolite of 17beta estradiol , has been reported to play an active role in the induction of apoptosis in both proliferating endothelial and cancer cells . ^^^ Since it has been indicated that an increased ratio of pro apoptotic Bax protein to anti apoptotic Bcl 2 protein expression can be associated with apoptosis , and since the exact action mechanism of 2ME is still not clearly defined and appears to vary according to cell type , the influence of 1 microM 2ME was investigated on Bax and Bcl 2 expression levels in squamous esophageal carcinoma cells . 2ME exposure led to statistically significant decreases ( 0 . 69 over DMSO controls ) in Bcl 2 expression levels . ^^^ The Bax / Bcl 2 ratio for 2ME exposed cells was 1 . 45 , normalised against Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis of T 24 cells by Chan Su was associated with a down regulation of anti apoptotic Bcl 2 and Bcl 10 ( S / L ) expression and an up regulation of pro apoptotic Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Experimentally , we found that anti apoptotic Bcl 2 protein levels decreased after ursolic acid treatment , while Bax expression increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further investigation for the underlying mechanism of such thymic atrophy revealed down regulation of anti apoptotic protein Bcl 2 and up regulation of pro apoptotic protein Bax , resulting in decreased Bcl 2 / Bax ratio thereby inducing apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This pathway is regulated by bcl 2 family of anti apoptotic ( bcl 2 , bcl xl , mcl 1 ) and pro apoptotic proteins ( bax , bad , bak ) . ^^^ The flow cytometric quantitative measurement of bcl 2 and bax expression for the determination of bax / bcl 2 ratio provided crucial clinical information in AML : in our hands , lower bax / bcl 2 ratio conferred a very poor prognosis with decreased rates of complete remission ( CR ) and overall survival ( OS ) . ^^^ Moreover , striking correlations were found between lower bax / bcl 2 ratio and higher progenitor marker expression , such as CD 34 , CD 117 and CD 133 antigens , confirming the link between this apoptotic index and the maturation pathways . ^^^ However , the capacity of bax / bcl 2 ratio to clearly identify patients with different prognosis with regard to CR and OS within the CD34+ , CD117+ and CD133+ subgroups implies that other mechanisms , such as proliferation and / or cell cycle dysregulation may be involved to explain its clinical significance . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , Bcl 2 , Bcl XL , Bid , cytochrome c and PKCd were detected by Western blotting . ^^^ A decreased expression of Bid was noted , although no significant changes in Bax , Bcl 2 and Bcl XL expression were observed after the combined treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
T 3 inhibited the apoptotic process induced by streptozocin , S Nitroso N Acetylpenicylamine ( SNAP ) , and H2O2 via regulation of the pro and anti apoptotic factors Bcl 2 , Bcl XL , Bad , Bax , and Caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results showed that Bcl 2 family molecules , such as Bid , Bak , and Bax , are involved in the parthenolide induced apoptosis and that the defective expression of Bcl 10 ( L ) might contribute to the higher parthenolide sensitivity in the SCK cells than in the other adenomatous cholangiocarcinoma cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR analysis for apoptosis related gene expression showed that the expression of Bax , Bcl 2 , Bcl xL , and Bcl xs genes , which are implicated in mitochondrial pathway , was significantly upregulated in the testes of the treated rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in ERK1 / 2 phosphorylation and in protein levels of Bcl 2 , Bax , and caspase 3 were assessed by Western blot analysis . ^^^ Bcl 2 levels progressively declined , whereas those of Bax and activated caspase 3 increased . ^^^ The observations also point to an imbalance in the expression of antiapoptotic ( Bcl 2 ) and proapoptotic ( Bax , caspase 3 ) proteins as a potential mechanism underlying adenosine induced mesangial cell apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although no significant changes in Bcl 2 expression occurred with ET 1 treatment , the pro apoptotic family members Bad , Bax , and Bak all decreased significantly . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The enhanced TRAIL effect after pretreatment with HDAC inhibitors was consistent with the upregulation of the proapoptotic Bcl 2 family members ( Bim , Bak , Bax , Noxa , and PUMA ) , the downregulation of the anti apoptotic members of the Bcl 2 family ( Bcl 2 and Bcl 10 ( L ) ) , and IAPs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
So we concluded that azurin could selectively induce apoptosis of human osteosarcoma U2OS cells and the induction of apoptosis by azurin was closely associated with down regulation of Bcl 2 , up regulation of Bax and activation of caspase 3 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ellipticine increased the expression of Bax , but decreased the level of Bcl 2 , Bcl XL and 10 linked inhibitor of apoptosis protein ( XIAP ) , and subsequently triggered the mitochondrial apoptotic pathway ( release of cytochrome c , and activation of caspase 9 and 3 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Most of the TUNEL positive cells displayed chromatin condensation and / or blebs and immunostained for increased Bax ; approximately 50 % of them were immunoreactive to cleaved caspase 3 and a few to Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Differential expression of apoptosis related genes ( e . g . apoptosis protease activating factor 1 [ Apaf 1 ] ; caspases [ casp ] 3 , 5 , 8 and 9 ; Bad , Bax , Bcl 2 , Bim , c myc , Bag 1 , as well as p 53 ) was assessed by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ RESULTS : Reverse transcription PCR revealed the detection of apoptosis related m RNAs as follows ( group A compared to group B ) : Apaf 1 0 % / 0 % ; Bcl 2 0 % / 35 % ; Bim 0 % / 9 % ; Bag 1 0 % / 9 % ; p 53 0 % / 4 % ; casp 3 11 % / 52 % ; casp 5 59 % / 48 % ; casp 8 44 % / 22 % ; casp 9 4 % / 9 % ; Bax 81 % / 52 % ; Bad 96 % / 56 % , and c myc 89 % / 96 % . ^^^ CONCLUSION : The data show an age related expression of apoptosis related genes such as casp 3 , Bad , Bax and Bcl 2 in normal conjunctival cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Combination of S allylcysteine and lycopene induces apoptosis by modulating Bcl 2 , Bax , Bim and caspases during experimental gastric carcinogenesis . ^^^ The present study was undertaken to investigate the apoptosis inducing potential of a combination of S allylcysteine ( SAC ) , an organosulphur constituent of garlic and lycopene , a tomato carotenoid during N methyl N ' nitro N nitroso guanidine ( MNNG ) and saturated sodium chloride ( S NaCl ) induced gastric carcinogenesis in Wistar rats using the apoptosis associated proteins Bcl 2 , Bax , Bim , caspase 8 and caspase 3 as markers . ^^^ Animals administered MNNG followed by S NaCl developed squamous cell carcinomas of the stomach associated with increased Bcl 2 expression and decreased expression of Bax , Bim , caspase 8 and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Trauma to the peripheral processes of sensory neurons of different subpopulations was followed by indirect immunohistochemical analysis of the expression of Bcl 10 ( L ) and Bax , which are , respectively , antiapoptotic and proapoptotic proteins of the Bcl 2 family , and also of the cytokine interleukin 1beta , with the aim of identifying the roles of these substances in controlling apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , sulforaphane induced apoptotic cell death was accompanied by upregulation of Bax and downregulation of Bcl 2 and Bcl 10 ( l ) protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the present study , the effects of EtOH on multiple functional mechanisms of Bcl 2 , Bcl xL , and Bax were investigated to characterize further the processes underlying these disparate EtOH sensitivities . ^^^ For these analyses , we addressed the following questions , by using P 4 and P 7 cerebellar tissue following in vivo exposure : 1 ) Are there differential patterns of expression of antiapoptotic Bcl 2 or proapoptotic Bax in EtOH vulnerable Purkinje cells that could contribute to the different degrees of temporal EtOH vulnerability . 2 ) How does EtOH affect intracellular localization of apoptosis related proteins . 3 ) Does cleavage of Bax contribute to EtOH sensitivity . 4 ) Does EtOH differentially modulate cerebellar protein protein interactions of Bcl 2 , Bcl xL , and Bax at the vulnerable vs . the resistant ages . ^^^ Overall , we show that , at P 4 , the EtOH mediated effects on Bcl 2 , Bcl xL , and Bax favor a prodeath response , whereas most of the intracellular responses to EtOH exposure at P 7 promote survival . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decreased mitochondrial membrane potential was indicated in TEPA treated cells , but further investigation of the potential induction of apoptosis by measuring caspase 3 activity , protein concentrations of Bcl 2 and Bax , and DNA fragmentation suggested that apoptosis is not induced in TEPA treated C2C12 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Fas / FasL , Bax / Bcl 2 , and c myc mRNA were measured by semi quantitative RT PCR in HL 7702 / HBx and control group , respectively . ^^^ By semi quantitative RT PCR analysis , Bax and c myc mRNA levels in HL 7702 / HBx cells of transient transfection were significantly higher than those in control , FasL and c myc mRNA levels in HL 7702 / HBx cells of stable transfection were significantly higher than those in control , whereas the Bcl 2 mRNA levels in HL 7702 / HBx cells of transient and stable transfection were significantly lower than those in control . ^^^ CONCLUSION : HBV 10 gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas / FasL , Bax / Bcl 2 , and c myc gene in a dose dependent manner . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Oxidative stress parameters , caspase 3 activity , and expression of Bcl 2 and Bax were determined to assess the probable mechanisms of altered apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot data revealed that NO gas increased the expressions of anti apoptosis proteins ( Bcl 2 and Mcl 1 ) and decreased the expression of apoptosis proteins ( Bax , t Bid , cytochrome c , FasL , and caspases ) after B [ a ] P treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Extensive investigation in the rats revealed that MNU induced photoreceptor cell loss was due to apoptosis with a decrease of Bcl 2 protein , increase of Bax protein , and activation of caspase families . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Cdc2 / Cdk 1 , cyclin B 1 , cyclin A , p21 / Cip1 , pRb , pRb2 / p130 , Bcl 2 , Bcl 10 ( L ) , Bax and caspase 3 proteins were studied with western blot analysis . ^^^ Apoptosis markers like Bcl 2 and Bcl 10 ( L ) were significantly decreased and Bax and caspase 3 were increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To investigate the effects of garlicin on apoptosis and expression of bcl 2 and bax in lymphocytes in rat model of ulcerative colitis ( UC ) . ^^^ On the 28th d of the experiment , rats were executed , the expression of bcl 2 and bax protein was determined immunohistochemically and the apoptotic cells were detected by the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate fluorescence nick end labeling ( TUNEL ) method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , antiapoptotic Bcl 2 and Bcl 10 ( L ) levels were increased and pro apoptotic Bax and Bak levels were decreased in the HIF 1alpha overexpressing OSCC line . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Results also displayed that PEITC induced apoptosis involves the up regulation of p 53 and Bax protein , down regulation of the XIAP , Bcl 2 , Bcl ( XL ) and Mcl 1 proteins , cleavage of Bid , and the release of cytochrome c and Smac / Diablo , which were accompanied by the activation of caspases 9 , 3 and 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antiapoptotic family proteins such as Bcl 2 and Bcl XL function , at least in part , by binding proapoptotic members such as Bax and Bak and thereby prevent release of the apoptotic cascade of events . `` BH 3 only ' ' members of the family disrupt this interaction by binding , via their BH 3 domain , to a hydrophobic pocket on the surface of the antiapoptotic members . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This chemo protective effect of BARF 1 was associated with increased Bcl 2 and Bax ratio and decreased expression of cleaved PARP , but not alterations in cell proliferation . ^^^ Our results suggest that BARF 1 expression in gastric cancer cells may provide a protective role against apoptosis through an increased Bcl 2 to Bax ratio , thus promoting cancer cell survival . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using a model of transient global ischemia , we have analyzed the NO system and the p 53 , bax and bcl 2 response in the cerebral cortex of aged rats . ^^^ Although immediately after ischemia the NO level is maintained , the reperfusion period increases NO concentrations together with the following : ( 1 ) greater bulk protein nitration mainly due to a 50 kDa immunoreactive band ; ( 2 ) an increase in p 53 protein ; and ( 3 ) an up regulation of Bax together with a down regulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We then investigated effects of puerarin on expression of apoptosis associated genes and the results revealed an increase of bax and decreases of c myc and bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we show proapoptotic BCL 2 family members BAX and BAK are essential for regulating the number of B cells at both immature and mature developmental stages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Of all flavonoids used only QU treatments led in both cell lines to DNA fragmentation , cleavage of poly ( ADP ribose ) polymerase ( PARP ) , up regulation of proapoptotic Bax and posttranslational modification ( phosphorylation ) of antiapoptotic Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Abnormalities of the P 53 , MDM 2 , BCL 2 and BAX genes in acute leukemias . ^^^ The purpose of this study was to define P 53 gene mutations , to detect MDM 2 gene amplification and to estimate mRNA expression of P 53 , MDM 2 , BCL 2 and BAX genes in patients with ALL and AML . ^^^ To gain further insight into the role of P 53 network in the evolution of acute leukemias , the P 53 , MDM 2 , BCL 2 and BAX mRNAexpressions in portion samples from patients with ALL and AML were analyzed using multiplex RT PCR . ^^^ A high frequency of BCL 2 mRNA overexpression and a relatively low frequency of BAX mRNA overexpression detected in both analyzed leukemias in this study , indicate that altered transcription of these genes may be involved in leukemogenesis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No cleavage of procaspase 3 , procaspase 9 and PARP , or altered expression of apoptotic proteins Bcl 2 and Bax were detected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of varicocele on expression of apoptosis associated gene Bcl 2 and Bax in spermatogenic cells of adolescent rats ] . ^^^ OBJECTIVE : To study the effect of varicocel ( VC ) on expression of apoptosis associated gene Bcl 2 and Bax in spermatogenic cells of adolescent rats , and investigate the mechanism of the infertility resulting from varicocele . ^^^ METHODS : The varicocele model was created by partial ligation of the left renal vein in the adolescent rats , two and four and eight weeks after creation of the varicocele model , the expressions of Bcl 2 and Bax were detected by immunohistochemical method . ^^^ CONCLUSION : Experimental varicocele could change obviously expression of apoptosis associated gene Bcl 2 and Bax in spermatogenic cells of adolescent rats . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that xanthoangelol markedly reduced the level of precursor caspase 3 and increased the level of cleaved caspase 3 , but Bax and Bcl 2 proteins were not affected . ^^^ These results suggest that xanthoangelol induces apoptotic cell death by activatation of caspase 3 in neuroblastoma and leukemia cells through a mechanism that does not involve Bax / Bcl 2 signal transduction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical staining for p 53 , p 21 , bax , bcl 2 , heat shock protein ( Hsp ) 27 and Hsp 70 was performed on the biopsy specimens before therapy . ^^^ Neither bcl 2 nor bax expression was related to the efficacy of therapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Diet had no effect on the uterine expression of genes for the tumor suppressors PTEN , p 53 and p 21 , and the apoptotic associated proteins Bcl 2 , Bax and progesterone receptor . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Methoxychlor directly affects ovarian antral follicle growth and atresia through Bcl 2 and Bax mediated pathways . ^^^ While in vivo studies indicate that MXC exposure increases antral follicle atresia , in part by altering apoptotic regulators ( Bcl 2 and Bax ) , they do not distinguish whether MXC does so via direct or indirect mechanisms . ^^^ Therefore , we utilized an in vitro follicle culture system to test the hypothesis that MXC is directly toxic to antral follicles , and that overexpression of anti apoptotic Bcl 2 , or deletion of pro apoptotic Bax , protects antral follicles from MXC induced toxicity . ^^^ Antral follicles were isolated from wild type ( WT ) , Bcl 2 overexpressing ( Bcl 2 OE ) , or Bax deficient ( BaxKO ) mice , and exposed to dimethylsulfoxide ( control ) or MXC ( 1 100 microg / ml ) for 96 h . ^^^ After 96 h , follicles were histologically evaluated for atresia or collected for quantitative PCR analysis of Bcl 2 and Bax mRNA levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Subsequent studies showed that combined treatment with bortezomib or MG 132 resulted in an increase of death receptor ( DR ) 5 and Bik at protein levels but had no effects on protein levels of DR 4 , Bax , Bak , Bcl 2 , Bcl XL or Flice inhibitory protein ( FLIP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis by I3C involves downregulation antiapoptotic gene products , including Bcl 2 , Bcl xL , survivin , inhibitor of apoptosis protein ( IAP ) , 10 chromosome linked IAP ( XIAP ) , and Fas associated death domain protein like interleukin 1 beta converting enzyme inhibitory protein ( FLIP ) ; upregulation of proapoptotic protein Bax ; release of micochondrial cytochrome C ; and activation of caspase 9 and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of estrogen receptor alpha ( ER alpha ) , progesterone receptor ( PR ) , Ki 67 , p 53 , bcl 2 , and bax were evaluated by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 gene expressions were evaluated by multiplex polymerase chain reaction . ^^^ At the same concentration , genistein also increased significantly Bax mRNA expression , but not Bcl 2 mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results show that the ITCs caused phosphorylation of Bcl 2 , induced mitochondrial translocation of Bak , and disrupted the association of Bcl xl with both Bak and Bax in mitochondrial membrane , indicating that ITC induced mitochondrial damage results at least in part from modulation of select Bcl 2 family members . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pristimerin did not significantly alter the protein level of Bcl 2 family members ( Bcl 2 , Bcl 10 ( L ) , and Bax ) , nor did it induce Bax translocation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally OPC treatment ( 100 microg / mL ) increased level of BAX and CASPASE 3 , and decreased level of BCL 2 mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIM : To explore the expression of p 53 , bcl 2 , bax , survivin and the cell apoptosis during the development of tree shrew hepatocellular carcinoma ( HCC ) , the relationship between expression of these genes , its impact on HCC development , and its relation to cell apoptosis . ^^^ Expression of p 53 , bcl 2 , bax , and survivin at each stage was examined by immunohistochemistry method . ^^^ Expression of the apoptosis related genes p 53 , bcl 2 , bax , and survivin during the formation of HCC presented an increasing tendency . ^^^ Expression of p 53 did not noticeably relate to that of bcl 2 , bax , and survivin , whereas expression of bcl 2 and bax was closely related . ^^^ CONCLUSION : Apoptosis related genes p 53 , bcl 2 , bax , and survivin are all related to the occurrence of HCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry and RT PCR techniques were used to measured the different expressions of the C Fos , Bcl 2 , Bax mRNA and protein among the groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Additionally , the downregulation of Bcl 2 , the activation of casapse 3 , as well as the upregulation of Bax were detected , suggesting the involvement of the caspase family and the activation of the mitochondrial signaling pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We found that knockdown of PKCepsilon did not affect the expression of Bcl 2 and Bax or the phosphorylation and expression of Erk1 / 2 , c Jun NH 2 kinase , p 38 , or STAT , whereas it selectively reduced the expression of AKT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The neuroprotective activity of these drugs has been demonstrated to be associated with the propargylamine moiety , which protects mitochondrial viability and mitochondrial permeability transition pore by activating Bcl 2 and downregulating the Bax family of proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objectives of this study were to investigate the effect of in utero VPA exposure on embryonic protein levels of p 53 , NF kappaB , Pim 1 , c Myb , Bax , and Bcl 2 in the CD 1 mouse . ^^^ Additionally , VPA increased the ratio of embryonic Bax / Bcl 2 protein levels ( P < 0 . 05 ) . ^^^ Folic acid also reduced VPA induced alterations in p 53 , NF kappaB , Pim 1 , c Myb , and Bax / Bcl 2 protein levels , while pantothenic acid prevented VPA induced alterations in NF kappaB , Pim 1 , and c Myb . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Similarly , the proapoptotic proteins Bax and BAD were significantly elevated , while the antiapoptotic proteins Bcl 2 , Bcl ( XL ) and Akt 1 were downregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the analysis of mice doubly deficient in multidomain Bcl 2 family proteins , Bax and Bak , revealed that apoptosis induced by the BH 3 only protein is completely dependent on Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The subcellular localizations of Bcl 2 , an antiapoptotic protein , and Bax , a proapoptotic protein , within RIN cells were altered with TNF alpha treatment such that the two were colocalized with mitochondria in the perinuclear region . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study shows that irradiation inhibited apoptosis by enhancing Bcl 2 , attenuating Bax induction , and preventing collapse of mitochondrial membrane potential ( delta psi ) , cytochrome c release into cytoplasm and caspase 3 , 6 and 9 activations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The BCL 2 family protein BAX is implicated in the formation of large `` death channels ' ' in outer mitochondrial membranes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proteins Bcl 2 and Bax were detected by immunoblot analysis . ^^^ Furthermore , ZnPc PDT could remarkably down regulate the Bcl 2 pro apoptotic protein and up regulate the anti apoptotic Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Antisense RIalpha but not CpG immunomer increased Bax and Bak proapoptotic protein levels and decreased Bcl 2 and RIalpha protein levels in tumor cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative RT PCR analysis revealed that ELF EMF exposure to ES derived neural cells significantly affected transcript levels of the apoptosis related bcl 2 , bax , and cell cycle regulatory `` growth arrest DNA damage inducible ' ' GADD 45 genes , whereas mRNA levels of neural specific genes were not affected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis related protein assays demonstrated that SNL glycoprotein induced apoptosis is associated with the regulation of bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Residual cytochrome c release in the absence of swelling in bcl 2 expressing cells could be due to observed Bax translocation into mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression pattern of pro and antiapoptotic proteins bax and Bcl 2 in rat brain neurons in response to severe hypobaric hypoxia : the correcting effect of hypoxic preconditioning . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To assess the reaction and participation of parenchymal brain cells during endotoxaemia , the present study evaluates micro and astroglial activation , expression of the inducible nitric oxide synthase ( iNOS ) pro and antiapoptotic protein levels Bax and Bcl 2 , and apoptosis . ^^^ Immunohistochemical evaluation revealed a sepsis induced , time dependent increase in the immunoreactivity of iNOS , glial fibrillary acidic protein ( GFAP ) and activated microglia ( ED 1 ) , paralleled by a time dependent increase of apoptotic brain cells marked by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) , an increase of Bax positive cells and a decrease of Bcl 2 positive cells . ^^^ In addition , it reduced the increase of the proapoptotic protein Bax in all examined brain regions and reduced the decrease of Bcl 2 positive cells in the hippocampus . ^^^ We therefore conclude , that peripheral inflammation leads to a profound glial activation , the generation of nitric oxide and changes of Bax and Bcl 2 protein regulation critical for apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activation of caspase 3 was assayed by FCM with FITC conjugated monoclonal rabbit anti active caspase 3 antibody and expressions of Bcl 2 and Bax proteins were examined immunocytochemically in 500 micromol / L TC induced apoptosis cells . ^^^ Immunocytochemical assay suggested that TC down regulated Bcl 2 protein level and up regulated Bax protein level . ^^^ Activation of caspase 3 , decreased Bcl 2 protein and increased Bax protein are involved in TC induced apoptosis of Eca 109 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Mice lacking JunD ( knockout [ KO ] ) showed increased mortality and enhanced cardiomyocyte apoptosis and fibrosis associated with increased levels of hypoxia induced factor 1alpha , vascular endothelial growth factor ( VEGF ) , p 53 , and Bax protein and reduced levels of Bcl 2 protein after 7 days of severe pressure overload compared with wild type ( WT ) siblings . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Overexpression of human GPX 1 modifies Bax to Bcl 2 apoptotic ratio in human endothelial cells . ^^^ In this study , we measured the expression of two Bcl 2 family members , Bax and Bcl 2 , in a human endothelial like cell line overexpressing the organic hydroperoxide scavenging enzyme glutathione peroxidase ( GPX 1 ) , in the absence of any apoptotic / oxidant stimulus . ^^^ ECV 304 were stably transfected with the GPX 1 cDNA and used for quantification of Bax ( pro apoptotic ) and Bcl 2 ( antiapoptotic ) mRNA and protein levels , by quantitative RT PCR and Western blot . ^^^ We found that , compared to control cells , cells from a clone showing a 13 . 2 fold increase in GPX 1 activity had unchanged mRNA or protein Bcl 2 levels but expressed 42 . 6 % and 46 . 1 % less Bax mRNA and Bax protein respectively . ^^^ Subsequently to Bax decrease , the Bax / Bcl 2 ratio , reflecting the apoptotic state of the cells , was also lower in cells overexpressing GPX 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although neither the total amounts nor the mitochondrial localization of Bax , Bcl 2 and Bcl xL were affected , caspase 3 activity was increased after NMDA exposure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for cell cycle regulators [ p 53 , p 21 , p 27 , p 16 , cyclin D 1 , Rb ] , apoptosis related proteins [ Fas , Fas L , FADD , TRAIL , DR 4 , DR 5 , caspase 8 , caspase 3 , bcl 2 , Bax ] , tumor suppressor proteins [ beta catenin , E cadherin , FHIT , Smad 4 , VHL , PTEN , KAI 1 ] , and oncoproteins [ c myc , COX 2 , EGFR ] . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Protein protein interactions within the Bcl 2 family are mediated by the helical BH 3 domain of proapoptotic family members , such as Bad , Bak , Bax , or Bid . ^^^ When added to the BH 3 domain derived from other proapoptotic proteins such as Bak , Bax , or Bid , these AEMs significantly increased the Bcl 2 binding affinity of these BH 3 peptides by promoting the helical structure . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis related genes Bcl 2 and Bax were used to check the possible mechanism of an estrogen or progesterone effect on survival of the cancer cell line . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we investigated the role of spontaneous apoptosis , which is regulated by death regulatory genes , such as Bcl 2 , Bax , p 53 , and isoenzymes of nitric oxide synthases ; eNOS and iNOS during the implantation window in women with unexplained infertility . ^^^ Apoptotic cells were detected using the dUTP nick end labelling assay and Bcl 2 , Bax , p 53 , iNOS and eNOS were assessed immunohistochemically . ^^^ Spontaneous apoptosis is reduced in the endometrium of unexplained infertile women , and is associated with the changed Bcl 2 : Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical expression of Ki 67 antigen , cox 2 and Bax / Bcl 2 in prostate cancer ; prognostic value in biopsies and radical prostatectomy specimens . ^^^ PURPOSES : To elucidate the prognostic value of the immunohistochemical ( IHC ) expression of Bcl 2 , Bax , Cox 2 and Ki 67 antigen in biopsy cores ( C ) and surgical specimens ( SP ) of prostate cancer ( PC ) and to determine the C to SP reproducibility . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was also a significant increase in the expression of Bax , Bcl 2 and Apaf 1 in SE rats over that of MD rats ; cytochrome c and AIF levels remained unchanged , however . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mechanism of morphine suppression of immunity might be through the suppression of E 7 specific CD8+ T lymphocyte proliferation and the promotion of apoptosis of these cells by the Bcl 2 and Bax pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of the extracellular matrix related genes , such as fibronectin , laminin and tenascin C , and apoptosis related genes , such as bax , bcl 2 and survivin , was evaluated by reverse transcription polymerase chain reaction ( RT PCR ) and by immunohistochemistry in normal breast tissue and benign and malignant breast tumors and then correlated to several clinical parameters : estrogen and progesterone receptors , Ki 67 , ErbB 2 , tumor size , lymph node status and grading . ^^^ After RNA extraction , an RT PCR was performed to detect fibronectin , laminin , tenascin C , bax , bcl 2 and survivin gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The induction of apoptosis was accompanied by the cleavage of caspase 3 , 8 , and 9 , as well as the downregulation of the Bcl 2 and the upregulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A literature search was undertaken using PubMed and MEDLINE search engines , using the keywords p 53 , p 21 , p 16 , p 27 , SMAD 4 , K ras , cyclin D 1 , Bax , Bcl 2 , EGFR , EGF , c erbB 2 , HB EGF , TGFbeta , FGF , MMP , uPA , cathepsin , heparanase , E cadherin , laminins , integrins , TMSF , CD 44 , cytokines , angiogenesis , VEGF , IL 8 , beta catenin , DNA microarray , and gene profiling . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the compound markedly reversed the apoptotic effects induced by long term serum withdrawal , including down regulation of the antiapoptotic protein , Bcl 2 , as well as up regulation of the proapoptotic proteins , Bax , Bad , and Bim . ^^^ Real time RT PCR demonstrated that propargylamine elevated gene expression levels of Bcl 2 , and the neurotrophic factors glial cell line derived neurotrophic factor ( GDNF ) and brain derived neurotrophic factor ( BDNF ) and reduced Bax gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fas , Fas ligand , p 53 , Bax and Bcl 2 expressions were analyzed by the respective monoclonal and polyclonal antibodies , using indirect immunofluorescence . ^^^ The incidence of apoptosis during PAN was correlated with the expression of AAP in glomerulus ( p 53 ) , interstitium ( Fas , Fas L and Bax ) and tubules ( Fas , Fas L , p 53 and Bcl 2 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Abeta ( 25 35 ) treatment upregulated pro apoptotic p 53 at the gene level , and Bax and caspase 3 at the protein level , but downregulated anti apoptotic Bcl 2 protein . ^^^ Interestingly , co treated green tea extract ( 10 50 microg / ml ) dose dependently attenuated Abeta ( 25 35 ) ( 50 microM ) induced cell death , intracellular ROS levels , and 8 oxodG formation , in addition to p 53 , Bax , and caspase 3 expression , but upregulated Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that GB 5 and GB 12 transiently increased the expression of both anti apoptotic protein ( Bcl 2 ) and proapoptotic proteins ( Bax and Bad ) in HL 60 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that sophorastilbene A [ 1 ] , piceatannol [ 5 ] and quercetin [ 9 ] did not induce any consistent changes in the expression of pro apoptotic proteins ( Bax , Bad ) and antiapoptotic protein ( Bcl 2 ) in HL 60 and HSC 2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The growth of the colo 201 human colon cancer cell line was examined by colorimetric 3 ( 4 , 5 dimethylthiazol 2 yl ) 5 ( 3 carboxymethoxyphenyl ) 2 ( 4 sulphophenyl ) 2H tetrazolium ( MTS ) assay , while the expression of apoptosis and proliferation related proteins ( p 53 , Bax , Bcl xL and S , Bcl 2 , Caspase 8 , Caspase 3 and proliferating cell nuclear antigen ( PCNA ) ) were examined by Western blotting . ^^^ The expression of an apoptosis suppressing protein ( Bcl 2 ) was down regulated , an apoptosis enhancing protein ( cleaved form of Caspase 3 ) was up regulated , proliferation related PCNA protein was down regulated and p 53 , Bax , Bcl xL and S and Caspase 8 levels were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There were no significant differences in total protein levels of caspase 3 , Bcl 2 , Bad , and Bax among the groups . iC BCP significantly induced greater phosphorylation of Bad ( 5 . 6+ / 0 . 8 fold ) as compared with the other 3 groups ( 3 . 4+ / 0 . 6 fold in iC CCP , P < 0 . 05 ; 2 . 5+ / 0 . 3 in iW BCP , P < 0 . 05 ; and 1 . 4+ / 0 . 2 in iW CCP , P < 0 . 01 ) . iC BCP induced less caspase 3 activation and apoptosis than the other 3 groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Finally , the analysis of the molecular markers that might be implicated in the synergism between LY 294002 and gemcitabine suggests that PI3K inhibition might aid chemotherapeutic treatment , leading to changes in the balance between anti and pro apoptotic molecules of the Bcl 2 family , Bcl XL and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 inhibition of apoptosis is mediated by its binding to pro apoptotic proteins , e . g . , Bax and tBid , inhibition of their oligomerization , and thus inhibition of mitochondrial outer membrane pore formation , through which other pro apoptotic proteins , e . g . , cytochrome c , are released to the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cell cycle perturbation and apoptosis were evaluated by flow cytometry , and apoptosis related marker ( p 53 , bcl 2 , bax , p 21 ) , caspase and thymidylate synthase ( TS ) expression were assessed by western blot . ^^^ The sequence Dox > Pacl > 48 h washout > 5 FU produced a synergistic and highly schedule dependent interaction ( combination index < 1 ) , resulting in an induction of apoptosis in both experimental models regardless of hormonal , p 53 , bcl 2 or bax status . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we examined the effects of heat shock on the proapoptotic Bcl 2 family member Bax because of its central role in regulating cytochrome c release in stressed cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was measured and the differential response of normal and cancer cells was correlated with the expression of bcl 2 , bax , GSH , and manganese superoxide dismutase ( MnSOD ) . ^^^ The ratio of bcl 2 : bax was decreased in the cancer derived cells treated with selenite . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effects on apoptosis and cell proliferation were investigated by histology and evaluation of apoptotic oncogenes Bcl 2 and Bax by Western Blot analysis . ^^^ Treatment with AN 207 , but not with AN 201 , decreased Bcl 2 / Bax ratio in DU 145 tumors and Bcl 2 in LuCaP 35 tumors indicating an increase in apoptotic activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A Western blot assay showed an acrylonitrile dependent induction of Bax , while Bcl 2 expression changed only after 48 h of chemical exposure . ^^^ Bax was overexpressed in respect to Bcl 2 , and this fact can be responsible for the induction in cell death after 24 h of treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic index or a combination of Bax / Bcl 2 expression correlate with survival after resection of pancreatic adenocarcinoma . ^^^ Expression analysis for p 53 , Bax , and Bcl 2 were performed by immunohistochemical staining . ^^^ By univariate analysis , overall survival seemed to be improved with Bcl 2 and Bax expression ( respectively , P = 0 . 0379 and 0 . 0311 ) . ^^^ Tumor ' s overexpression of both Bax and Bcl 2 resulted the strongest independent prognostic factor ( P = 0 . 013 ) . ^^^ The contemporary overexpression of Bax and Bcl 2 represents the strongest prognostic factor . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial membrane permeabilization induces the release of cytochrome c , Smac / DIABLO , and AIF , which are regulated by proapoptotic and antiapoptotic proteins such as Bax / Bak and Bcl 2 / xL in caspase dependent and caspase independent apoptosis pathways . ^^^ The first is by increased calcium in mitochondria derived from endoplasmic reticulum ( ER ) ; this calcium increase is regulated by Bcl 2 and Bax through the ER mitochondria connection and the unfolded protein response in the ER . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : Twenty breast cancer patients who were categorized as pre and postmenopausal and 20 mammary gland tumors obtained from dogs were included in this study The expression of Bcl 2 , Bcl 10 ( L ) , Bax , caspases 8 and 3 as well as Hsp 70 and 90 in tumor tissues and adjacent tissues were investigated using Western blotting . ^^^ RESULTS : While expression of Bcl 2 , Bcl 10 ( L ) , Hsp 70 and 90 was increased , expression of Bax and caspases 8 and 3 was significantly lower in both human as well as canine mammary tumor tissues compared to corresponding adjacent tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this case , mitochondrial Bcl 2 family , Bcl 2 and Bax , were not involved in apoptotic effects ; however , silymarin induced cell death was regulated by the activation of p 38 and JNK MAPKs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 family members Bax and Bid then form pores in the outer mitochondrial membrane and release intermembrane proteins , e . g . , apoptosis inducing factor ( AIF ) and endonuclease G , which then translocate to the nucleus and initiate chromatin condensation and DNA fragmentation , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NPD 1 is synthesized in RPE cells undergoing oxidative stress , potently counteracts oxidative stress triggered apoptotic DNA damage in RPE , upregulates antiapoptotic proteins Bcl 2 and Bcl 10 ( L ) , and decreases proapoptotic Bax and Bad expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This redox shift was kinetically linked to decreased BcL 2 , increased Bax , and release of mitochondrial cytochrome c which preceded caspase 9 and 3 activation and poly ADP ribose polymerase ( PARP ) cleavage ( 1 2 h ) , consistent with mitochondrial apoptotic signaling . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NaF enhanced the expression of Bad protein , but not that of Bcl 2 and Bax proteins , and reduced HIF 1alpha mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Glatiramer acetate induces pro apoptotic mechanisms involving Bcl 2 , Bax and Cyt c in peripheral lymphocytes from multiple sclerosis patients . ^^^ Peripheral blood mononuclear cells ( PBMNCs ) Bcl 2 , Bax , Cyt c and APAF 1 were quantified by western blot followed by densitometric scanning and Bax / Bcl 2 , cytosolic Cyt c / Bcl 2 and APAF 1 / Bcl 2 ratios were calculated . ^^^ Bax / Bcl 2 , cytosolic Cyt c / Bcl 2 and APAF 1 / Bcl 2 ratios in untreated MS patients were significantly ( p < 0 . 05 ) lower than in HCs . ^^^ Bax / Bcl 2 ratio increased ( p = 0 . 03 ) and Cyt c / Bcl 2 ratio showed a trend to increase during the 9 months of GA treatment in MS patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The objective was to evaluate and compare the bcl 2 , bax , and nestin patterns in these three groups . ^^^ Using immunocytochemistry , it was observed that bcl 2 and bax active sites were colocalized in 45 % of cells in Alzheimer , 52 % of cells in multiple infarct , and 30 % of cells in normal aging . bcl 2 and bax could also be separately located in cells of all three groups . bax cells were most prominent in number in Alzheimer patients and least prominent in normal aging . nestin was found in all three groups but was most prominent in the multiple infarct patients . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA and protein levels of three apoptosis related genes ( p 53 and bax were promoters of apoptosis , whereas bcl 2 was apoptotic suppressor ) were analyzed in livers using a real time reverse transcription polymerase chain reaction ( real time RT PCR ) assay and immunohistochemistry method . ^^^ The results showed that mRNA levels of p 53 and bax were increased in a dose dependent manner and at the concentrations of 28 . 00 and 56 . 00 mg / m3SO2 the increases were significant ( for p 53 : 1 . 30 fold at 28 mg / m3 and 3 . 43 fold at 56 mg / m3 , for bax : 1 . 63 fold at 28 mg / m3 and 2 . 17 fold at 56 mg / m3 , respectively ) , while mRNA levels of bcl 2 were decreased significantly ( 0 . 63 fold at 28 mg / m3 and 0 . 45 fold at 56 mg / m3 ) in livers of rats exposed to SO 2 . ^^^ Dose dependent increases of p 53 and bax proteins in the livers were observed after SO 2 inhalation , while decrease of bcl 2 protein levels was obtained using immunohistochemistry method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The anti and pro apoptotic proteins Bcl 2 and Bax were also evaluated . ^^^ No correlation was found between Fas and Bcl 2 expression but a good association was obtained with Bax , in the subgroup with increased Fas expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax were identified by RT PCR and bax was also immunolocalized in tissue sections . ^^^ Both bcl 2 and bax were found to be more highly expressed in the perichondrium / mesenchyme and non mineralized cartilage than in skin and mineralized cartilage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblot analysis revealed that PFE treatment of PC 3 cells resulted in ( 1 ) induction of Bax and Bak ( proapoptotic ) ; ( 2 ) down regulation of Bcl 10 ( L ) and Bcl 2 ( antiapoptotic ) ; ( 3 ) induction of WAF1 / p21 and KIP1 / p27 ; ( 4 ) a decrease in cyclins D 1 , D 2 , and E ; and ( 5 ) a decrease in cyclin dependent kinase ( cdk ) 2 , cdk 4 , and cdk 6 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoblotting analysis showed no upregulation of pro apoptotic proteins ( caspase 2L , 3 , 6 , 8 , 9 , and Bax ) with age while all the anti apoptotic proteins ( caspase 2S , Bcl 2 , and Bcl XL ) remained unchanged during aging . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , we correlated expression of these proteins with the apoptosis related proteins p 53 , bcl 2 , and bax . ^^^ METHODS : Consecutive sections from 60 cases of ovarian serous carcinoma were assessed immunohistochemically for expression of P gp , MRP 1 , LRP , p 53 , bcl 2 , and bax . ^^^ RESULTS : Strong P gp expression was observed in 12 ( 20 % ) , MRP 1 in 39 ( 65 % ) , LRP in 27 ( 45 % ) , p 53 in 45 ( 75 % ) , bcl 2 in 25 ( 41 . 7 % ) , and bax in 30 ( 50 % ) of the 60 tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show that the gamma herpesvirus 68 ( gammaHV 68 ) Bcl 2 family protein ( gammaHV 68 5 Bcl 2 ) , which is known to inhibit apoptosis in cultured cells , inhibits both apoptosis in primary lymphocytes and Bax toxicity in yeast . ^^^ Nuclear magnetic resonance determination of the gammaHV 68 5 Bcl 2 structure revealed a BH 3 binding groove that binds BH 3 domain peptides from proapoptotic Bcl 2 family members Bax and Bak via a molecular mechanism shared with host Bcl 2 family proteins , involving a conserved arginine in the BH 3 peptide binding groove . ^^^ Mutations of this conserved arginine and two adjacent amino acids to alanine ( SGR to AAA ) within the BH 3 binding groove resulted in a properly folded protein that lacked the capacity of the wild type gammaHV 68 5 Bcl 2 to bind Bax BH 3 peptide and to block Bax toxicity in yeast . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The efficacy of chemotherapeutic agents on tumor cells has been shown to be modulated by tumor suppressor gene p 53 and its target genes such as Bcl 2 family members ( Bax , Noxa , and PUMA ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The oxalomalate pre treated cells showed significant enhancement of apoptotic features such as activation of caspase 3 , up regulation of Bax , and down regulation of Bcl 2 compared to the control cells upon exposure to heat shock . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlation between proliferation and apoptosis in different histological types of uterine leiomyoma ] . 75 cases of simple , cellular and mitotically active leiomyoma ( LM ) were studied immunohistochemically with the use of monoclonal antibodies against bcl 2 ( `` DAKO ' ' , 1 : 80 ) , bax ( `` Calbiochem , 1 : 80 ) , c myc ( `` Novocastra ' ' , 1 : 100 ) , CD 95 ( `` Dianova , 1 : 50 ) , Ki 67 ( `` Dianova ' ' , 1 : 40 ) , PCNA ( `` Novacastra ' ' , 1 : 100 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In order to understand the mechanism of apoptosis induction by eutigoside C , we examined the changes of Bcl 2 and Bax expression levels . ^^^ The eutigoside C reduced Bcl 2 protein and mRNA levels , but slightly increased Bax protein and mRNA levels in a time dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This phenomenon is paralleled by increased expression of adhesion molecules ( CD 106 and CD62p ) and activation of apoptotic pathways ( Bax and Bcl 2 ) in islet cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the activation of caspase 8 and Bax , the inhibition of Bcl 2 expression , and the release of cytochrome c are believed to be involved in the RCMF mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hyperhomocysteinemia caused an elevation of pro apoptotic Bax levels while reducing anti apoptotic protein , Bcl 2 , levels . ^^^ Daily administration of melatonin up regulated Bcl 2 and down regulated Bax levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Meanwhile , ATA increased the expression of Bax , decreased the expression of Bcl 2 , and lowered the DeltaPsi ( m ) . ^^^ These results indicate that ATA could significantly induce cell apoptosis through down regulating Bcl 2 expression , up regulating Bax expression , lowering DeltaPsi ( m ) , and activating the caspase 3 pathway , and should be useful in the search for new potential anti tumor agents and for developing semisynthetic oleanane type triterpene derivatives with anti tumor activity . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot and multiple RT PCR analysis revealed that the anti apoptotic bcl 2 was upregulated at both protein and mRNA levels , together with the downregulation of pro apoptotic bax . ^^^ The pro proliferative activity of ZEA was due to inhibition of apoptosis through regulation of bax / bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , this treatment resulted in a significantly increased RPWAT apoptotic index and Bax protein expression and a decreased expression of Bcl 2 in the lean rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , staining for survivin , Bcl 2 , Bax , caspase 3 and Ki 67 was performed in prostate specimens from 23 consecutive patients who underwent open prostatectomy and 4 young patients who underwent cystoprostatectomy and had a normal prostate . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptotic activity was evaluated by terminal deoxynucleotidyltransferase mediated dUTP nick end labeling staining and semi quantification of Bax and Bcl 2 expression by real time RT PCR and western blotting . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ectopic expression of Bcl 2 or Bcl 10 ( L ) was also unable to prevent the flavopiridol / HDACI regimen from inducing a conformational change in and mitochondrial translocation of Bax , and it did not attenuate Bax dimerization . ^^^ As a whole , these findings indicate that in contrast to certain conventional cytotoxic agents such as ara C , overexpression of Bcl 2 or Bcl 10 ( L ) are largely ineffective in preventing perturbations in Bax , mitochondrial injury , and cell death in human leukemia cells subjected to simultaneous CDK and HDAC inhibition . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PSI induced cell death required RNA transcription and protein synthesis , but not DNA replication , was accompanied by the upregulation of Bcl 2 and modest reduction of Bax and Bcl XL proteins , and involved the activation of caspases 2 , 3 , 7 and 8 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
LS and RLS strains do not differ by the expression of bcl 2 , bcl 6 , bax , bad , mdr1a , mdr1b genes and P glycoprotein protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Patterns of expression of Bax , Bcl 2 and Bcl 10 ( L ) in the implantation site in rat during pregnancy . ^^^ Overall Bax and Bcl 2 were expressed from day 8 till day 19 , whilst Bcl 10 ( L ) was extinguished by day 16 . ^^^ In the antimesometrial and in the mesometrial decidua both Bcl 2 and Bax declined from days 10 to 12 . ^^^ In the latter Bcl 2 immunoreactivity decreased till the end of pregnancy , whilst for Bax a constant level remained thereafter . ^^^ The increased levels of active caspase 9 correlated with Bax / Bcl 2 and Bcl 10 ( L ) expression suggesting that the apoptotic mitochondrion dependent pathway is involved in decidual regression during pregnancy progression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although mechanistically different , both transcription independent modes of apoptosis induction converge , as they both initiate permeabilization of the outer mitochondrial membrane via activation of the pro apoptotic Bcl 2 family members Bax or Bak . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Coadministration of sulindac sulfide and the small molecule Bcl 2 inhibitor HA 14 1 increased apoptosis induction and enhanced caspase 8 and caspase 9 cleavage , Bax redistribution , and cytochrome c and second mitochondria derived activator of caspase release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The recruitment of the p 53 dependent apoptosis pathway was suggested by the up regulation of p 53 , p 21 , Bax , DR 4 , DR 5 , and p 53 phosphorylated on Ser 15 ; down regulation of Bcl 2 ; and activation of caspase 8 , 9 , 7 , and 3 in cells treated with 100 nmol / L WMC 79 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bcl xL expressions were down regulated after paclitaxel treatment in FHIT expressing cells , whereas Bax and Bad expressions were up regulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of human interleukin 10 gene transfer on the expression of Bcl 2 Bax and apoptosis of hepatocyte in rats with acute hemorrhagic necrotizing pancreatitis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The intensities of expression of p 53 , Ki 67 , Bcl 2 , Bax , cyclin D 1 , VEGF , CDC25B , and metallothionein ( MT ) were evaluated immunohistochemically in the biopsy specimens obtained before CRT , and the intensities of their expression were tested for correlations with the clinical effects of CRT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of subchronic exposure to acrylamide induced on the expression of bcl 2 , bax and caspase 3 in the rat nervous system . ^^^ We hypothesize that ACR differentially affects the expression of bcl 2 , bax and caspase 3 in the central nervous system ( CNS ) and the peripheral nervous system ( PNS ) tissue . ^^^ Samples of the cerebral cortex , cerebellum , spinal cord and sciatic nerves were collected and examined for bcl 2 , bax and caspase 3 expression using Western blotting . ^^^ Subchronic exposure to ACR reduced cortical bcl 2 expression in the low dose , increased it in the high dose ; the change of bcl 2 expression in the spinal cord and cerebellum followed the same pattern as that described in the cerebral cortex ; there was no significant change in the expression of bax in the cerebral cortex and the spinal cord , however , in the cerebellum the change of bax expression and bcl 2 expression is just the reverse . ^^^ Thus , the bcl 2 / bax ratio of the CNS tissue was affected by exposure to ACR , it decreased in the low dose group and increased in the high group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We checked the methylation status of 20 cancer associated genes ( p14ARF , p 16 CDKN2A , STAT 1 , SOCS 1 , DR 3 , DR 6 , PIG 7 , BCL 2 , H TERT , BAX , EDNRB , DAPK , RASSF 1A , FADD , TMS 1 , E Cadherin , ICAM 1 , TIMP 3 , MLH 1 , COX 2 ) for DNA methylation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated the apoptotic effects of polyphenols from red wine on human colon cancer cells SNU C 4 using 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide ( MTT ) assay , 4 , 6 diamidino 2 phenylindole ( DAPI ) staining , terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP nick end labeling ( TUNEL ) assay , reverse transcription polymerase chain reaction ( RT PCR ) expressions of Bcl 2 , Bax and Caspase 3 genes , and Caspase 3 enzyme activity . ^^^ Compared with untreated control group , polyphenols ( 100 microg / ml ) reduced the expression of Bcl 2 whereas those of Bax and Caspase 3 were increased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further investigation showed that silencing of hPEBP 4 in MCF 7 cells promoted TNF alpha induced stability of p 53 , up regulation of phospho p53ser15 , p21waf / cip , and Bax , and down regulation of Bcl 2 and Bcl xL , which were shown to depend on extracellular signal regulated kinase 1 / 2 and c jun NH 2 terminal kinase activation by hPEBP 4 silencing . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bax , Bad and Bcl 2 proteins under 10 radiation effect towards human breast carcinoma MCF 7 cells and their doxorubicin resistant derivatives . ^^^ AIM : 1 ) To investigate the effect of 10 rays on expression of Bax , Bad , and Bcl 2 proteins in human breast carcinoma MCF 7 cells , sensitive ( MCF 7 ( wt ) ) and resistant ( MCF 7 ( DOX / R ) ) to doxorubicin ; 2 ) to measure 10 radiation induced DNA damage in order to clarify if changes in the expression of those proteins and DNA repair capacity can be responsible for the development of drug resistance and for altered radio sensitivity of MCF 7 ( DOX / R ) cells . ^^^ METHODS : Expression of Bax , Bad and Bcl 2 proteins was detected by Western blot analysis . ^^^ RESULTS : We found an increase in the expression of pro apoptotic Bax and Bad proteins and a decrease in the expression of Bcl 2 protein in MCF 7 ( wt ) cells under the effect of 10 radiation , whereas the expression of those proteins was not changed significantly in the irradiated MCF 7 ( DOX / R ) cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bcl 2 protein expression in gastric precancerous lesions : immunohistochemical study . ^^^ The aim of our study was to determine the roles of the Bax proapoptotic gene and the Bcl 2 antiapoptotic gene in the carcinogenesis of gastric cancer . ^^^ Using immunohistochemical methods , Bax and Bcl 2 protein expression was observed . ^^^ CONCLUSIONS : The suppression of Bax and overexpression of Bcl 2 protein is an early event in gastric tumorigenesis , before gastric dysplastic changes occur . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of other Bcl 2 family proteins ( Bcl 2 , Bcl 10 ( L ) , Bad , Bak , Bax ) were unaffected by simultaneous ATRA and TGF beta 1 treatment , when compared to ATRA alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Such induction was found to be mediated through activation of caspases 3 and 8 , particularly caspase 3 and by altering apoptosis related genes as evident by down regulation of Bcl 2 and up regulation of Bax proteins . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : BCL 2 and BAX are important in the regulation of apoptosis . ^^^ There have been reports of loss of BCL 2 in basal cells of oral epithelial dysplasia ( OED ) and in oral squamous cell carcinoma ( OSCC ) , and suppression of BAX in poorly differentiated OSCC . ^^^ AIM : To investigate whether loss of BCL 2 in OED and OSCC , and of BAX in poorly differentiated OSCC could be attributed to BCL 2 and BAX mutations . ^^^ METHODS : Immunohistochemistry and in situ hybridisation were used to confirm BCL 2 and BAX expression . ^^^ RESULTS : No mutations were detected in BCL 2 or BAX that could explain their aberrant expression at the mRNA and protein levels in OED and OSCC . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA and protein levels of three apoptosis related genes ( p 53 and bax are promoters of apoptosis , whereas bcl 2 is apoptotic suppressor ) were analyzed in lungs using a real time reverse transcription polymerase chain reaction ( real time RT PCR ) assay and immunohistochemistry method , and caspase 3 activities were detected . ^^^ The results showed that mRNA levels of p 53 and bax were increased in a dose dependent manner and at the concentrations of 28 . 00 and 56 . 00 mg / m3 SO 2 the increases were significant ( for p 53 : 1 . 23 fold at 28 mg / m3 and 1 . 39 fold at 56 mg / m3 ; for bax : 1 . 77 fold at 28 mg / m3 and 2 . 26 fold at 56 mg / m3 , respectively ) , while mRNA levels of bcl 2 were decreased significantly ( 0 . 78 fold at 28 mg / m3 and 0 . 73 fold at 56 mg / m3 ) in lungs of rats exposed to SO 2 . ^^^ Dose dependent increase of p 53 and bax proteins in the lungs was observed after SO 2 inhalation , while decrease of bcl 2 protein levels was obtained using immunohistochemistry method . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BCL XL , BAX and BCL 2 are members of the BCL 2 protein family known to regulate both apoptotic and necrotic cell death signaling at the mtPTP . ^^^ No significant treatment related alterations in BCL XL , BAX or BCL 2 protein expression are observed in rat tissue homogenates or primary astrocytes . ^^^ However , moderate increases in BCL XL are observed only in DNB treated rat cortical astrocytes , and these increases may be sufficient to shift the constitutive balance in expression of antagonistic to agonistic BCL 2 proteins from a ratio which favors BAX to one in which BAX and BCL XL are comparably expressed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mechanistically , polyphyllin D dissipates the mitochondrial membrane potential , induces a downregulation of anti apoptotic Bcl 2 expression and an up regulation of pro apoptotic Bax expression , and activates caspase 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study was performed to examine the hypothesis that Stx initiates apoptosis by activating the mitochondrial pathway involving mitochondrial associated , pro apoptotic Bcl 2 family proteins Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Upregulation of caspase 1 , 2 , 3 , 6 , 7 , 8 , 11 and 12 and upregulation for the transcripts of apoptosis inhibitors bcl 2 , bcl w and bcl 10 and apoptosis promoters ' bax , bak and bad was detected in spleens of sPCV and mPCV mice , but not control mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot showed that LPS in high glucose increased the levels of anti apoptotic Bcl 2 and Bcl 10 ( L , ) and did not change proapoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
CPP 32 , Bcl 2 , and Bax ) . ^^^ We also used immunohistochemical techniques to examine the levels of CPP 32 , Bcl 2 , and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this way , we have analyzed in the present work the effects of PCBs on protein kinase C ( PKC , a protein family intimately involved in the regulation of cell survival ) and the expression of two proapoptotic ( caspase 3 and Bax ) and one antiapoptotic ( Bcl 2 ) proteins . ^^^ In addition , Aroclor 1248 decreased both , procaspase 3 levels and the Bcl 2 / Bax protein ratio . ^^^ These findings indicate that PCBs , particularly nonplanar congeners , can induce apoptosis in primary renal tubular cells through the PKCalpha , caspase 3 and Bcl 2 / Bax pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantification of Bcl 2 and Bax proteins in OOX was undertaken by western analysis . ^^^ Accordingly , levels of anti apoptotic Bcl 2 were high in OOX+DO and OOX+BMP15 and low with OOX+GDF9 or OOX alone , whereas the reverse was observed for pro apoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was associated by a decrease of the antiapoptotic protein Bcl 2 , an increase of the pro apoptotic protein Bax , and activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of Bcl 2 and Bax was related to hyperphosphorylation of tau and neuronal death induced by okadaic acid in rat brain . ^^^ In the present study using a model of microinjection of OA into rat frontal cortex , we aimed to investigate if OA induced hyperphosphorylation of tau and neuronal death are related to the expression of Bcl 2 , an apoptosis inhibitor , or Bax , an apoptosis inducer . ^^^ Immunohistochemistry and Western blot analysis showed that OA injection dose and time dependently induced the expression of Bcl 2 and Bax protein in the surrounding of OA injection areas , which were similar with that of AT 8 immunostaining , a marker of hyperphosphorylated tau . ^^^ However , the ratios of Bcl 2 over Bax had a negative relationship to the expression of AT 8 . ^^^ In the areas more adjacent to the OA injection site , most neurons with AT 8 positive staining showed vulnerability to OA toxicity and could be triple stained with Bcl 2 and Bax or double stained with Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , addition of Gl PP also led to a reduction of Bcl 2 anti apoptotic protein expression and an increase of Bax pro apoptotic protein expression of HUVECs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proliferation and apoptosis related transcription factor immunoexpression patterns were concomitantly investigated in tissue sections of normal thyroid , goiters , follicular adenomas and well differentiated papillary and follicular carcinomas using antibodies against prothymosin alpha , E2F 1 , p 53 , Bcl 2 , and Bax proteins . ^^^ Direct correlations were observed between prothymosin alpha and Bcl 2 as well as between E2F 1 and Bax immunoexpression ( P < 0 . 05 ) . ^^^ Furthermore , prothymosin alpha may promote cell survival through the Bcl 2 anti apoptotic pathway , while E2F 1 induced apoptosis via p 53 independent pathways may be associated with transcriptional activation of bax pro apoptotic gene . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , a higher dose of oridonin promoted p 53 phosphorylation , increased Bax expression and subsequently induced death of low sensitive L 929 cells , however , it had no effect on Bcl 2 expression . ^^^ The increased Bcl 2 / Bax ratio in oridonin low sensitive L 929 cells did not inhibit caspase 9 or 3 activation , but suppressed the cleavage of poly ( ADP ribose ) polymerase ( PARP ) , indicating the existence of caspase 9 or 3 independent PARP activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using tissue microarrays ( TMAs ) , we have analysed the immuno expression of Ki 67 , Bcl 2 , Bax , cyclin D 1 , cyclin D 3 , CDK 1 , CDK 2 , CDK 6 , p 16 , p 21 , and p 27 in a series of 205 carcinomas of the large bowel , breast , lung and prostate ( 80 , 73 , 37 and 15 cases , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression profiles of genes , c myc , AT 1 , AT 2 , ETA , ETB , Bcl 2 , Bax and Bcl 10 , were determined by reverse transcription polymerase chain reaction ( RT PCR ) in the acute phase , from 1 to 48 h , following CA arteriotomy . ^^^ Bcl 2 / Bax ratio gradually decreases in WKY , while it decreases only transiently in SHR and MHS 4 h after injury ( p < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
HDAC inhibitors enhance the apoptosis inducing potential of TRAIL in leukemia cells ( HL 60 , Jurkat , K 562 , and U 937 ) through multiple mechanisms ; up regulation of DR 4 , DR 5 , Bak , Bax , Bim , Noxa and PUMA , down regulation of IAPs , Mcl 1 , Bcl 2 , Bcl XL and cFLIP , release of mitochondrial proteins ( cytochrome c , Smac / DIABLO and Omi / Htr2 ) to the cytosol , induction of p21WAF1 / CIP1 and p27KIP1 , activation of caspase 3 and cleavage of poly ( ADP ribose ) polymerase ( PARP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes of expressions in immediate early genes ( IEGs ) , glutamate receptors ( GluRs ) , calcium binding proteins ( CaBPs ) , 8 hydroxy deoxyguanosine ( 8 OH dG ) and nitric oxide synthase ( NOS ) , as well as apoptotic related factors including caspase 3 , bax , and bcl 2 were examined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We took out blood and did hemorrheologic examination and erythrocyte osmotic fragility test , checked up the water content , capillary permeability , and genetic expression of intercellular adhesion molecule 1 ( ICAM 1 ) in lung tissues , examined the apoptosis degree of blood vessel endothelium while we tested related gene expression of Bax and Bcl 2 in lung tissues . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Guggulsterone induced apoptosis was associated with induction of multidomain proapoptotic Bcl 2 family members Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Polymorphonuclear cell exposure to PVL leads to mitochondrial localization of the toxin , whereas Bax , 1 of the 2 essential proapoptotic members of the Bcl 2 family , was still localized in the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Up regulation of Bad and p 21 ; down regulation of Bcl 2 , Bcl XL , Bid , p 53 , and fatty acid synthase ; and cleavage of Bax were found in HMDB treated A 431 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Multicolor flow cytometry was used to evaluate CCR 7 , Fas , Bax , and Bcl 2 expression in CD 8 ( + ) T cells . ^^^ CCR 7 expression correlated with higher Bcl 2 but lower Bax and Fas expression levels in CD 8 ( + ) T cells in both normal control and patients ( P < 0 . 0001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This occurred in the setting of increased Bax and cleaved poly ( ADP ribose ) polymerase , as well as decreased Bcl 2 and pAkt . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also demonstrated a concurrent decrease of the mitochondrial membrane potential , release of cytochrome c , up regulation of Bax , down regulation of Bcl 2 , and activation of caspase 3 , and subsequently , cleavage of poly ( ADP ribose ) polymerase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effects of liuwei dihuang pills on expressions of apoptosis related genes bcl 2 and Bax in pancreas of OLETF rats ] . ^^^ OBJECTIVE : To investigate the effects of Liuwei Dihuang Pills ( LWDHP ) on expressions of apoptosis related genes bcl 2 and Bax in pancreas of OLETF rats . ^^^ Pancreas weights were recorded after rats were killed and the expression levels of bcl 2 mRNA and Bax mRNA were detected by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ CONCLUSION : LWDHP can up regulate the expression of bcl 2 and down regulate the expression of Bax at transcription level , which maybe contribute to the anti apoptosis effects of LWDHP . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To study the effects of Yiqi Huayu Recipe and its decomposed formulas medicated sera on expressions of bcl 2 , Bax and caspase 8 of apoptotic anulus fibrosus cells in rats . ^^^ METHODS : Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi Huayu Recipe , Yiqi Recipe , Huayu Recipe medicated sera and insulin like growth factor 1 ( IGF 1 ) on the expressions of bcl 2 , Bax and caspase 8 of apoptotic anulus fibrosus cells in rats induced by anti Fas antibody . ^^^ RESULTS : As compared with apoptosis group , bcl 2 expression was higher , Bax and caspase 8 expressions were lower in Yiqi Huayu treated , Yiqi treated , Huayu treated and IGF 1 groups ( P < 0 . 01 ) . ^^^ CONCLUSION : Yiqi Huayu Recipe and its decomposed formulas can delay degeneration of the cervical intervertebral disc , which may be due to its action in regulating the expressions of bcl 2 , Bax and caspase 8 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treated with conventional anticancer drugs or radiation , MDA 7 expressing cells display additive or synergistic cytotoxicity and apoptosis that correlates with decreased BCL 2 expression and BAX upregulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of p 53 and Bax ( pro apoptotic protein ) , Bcl 2 ( anti apoptotic protein ) , Fas , and Fas L ( apoptotic death receptor and ligand ) , caspase 3 ( apoptotic effector caspase proteins ) , and p 27 and p 21 ( cyclin dependent kinase inhibitors ) were analyzed using tissue array blocks . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptotic cascade triggered by 5 HT involves release of cytochrome c , upregulation of proapoptotic Bax protein , and downregulation of antiapoptotic Bcl 2 protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At 30 min , 2 h and 1 week after bladder emptying , samples of bladder tissue were taken for muscle strip study , malondialdehyde ( MDA ) assay , ATP assay , Western blotting for apoptosis related molecules ( Bcl 2 , Bax , Caspase 3 ) , and histological analysis including terminal deoxynucleotidyl transferase mediated nick end labelling staining . ^^^ While pro apoptotic Bax and caspase 3 were down regulated , Bcl 2 was up regulated in the glycine treated group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Combined gene knockout with RNAi cosilencing experiments indicate that SIRT 1 and Bcl 2 may suppress separable apoptotic pathways in the same cell lineage and that the SIRT 1 regulated pathway is independent of p 53 , Bax , and caspase 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we used Bax protein , a pro apoptotic member of the Bcl 2 family of proteins , as a model protein to investigate the conformational alteration triggered by a TNF related apoptosis inducing ligand ( TRAIL ) , a potent inducer of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVES : The primary aim of this study was to investigate the expression of apoptotic and cell cycle regulators p 53 , p 21 , p 27 , bax , and bcl 2 in uterine leiomyosarcoma in order to identify molecular pathways that possibly could be important in the development of leiomyosarcoma . ^^^ Immunohistochemical staining was performed for bcl 2 , bax , p 21 , p 27 , and p 53 using standard techniques . ^^^ RESULTS : Associations were found between disease type ( leiomyosarcoma vs . leiomyoma ) and the positivity status of p 21 ( 43 % vs . 0 % , P < 0 . 001 ) , p 53 ( 54 % vs . 0 % , P < 0 . 001 ) , and bax ( 34 % vs . 94 % , P < 0 . 001 ) . bcl 2 positive leiomyosarcoma was associated with a longer time to recurrence ( P = 0 . 02 ) in a univariate analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results suggest that the protective effects of DNP against I / R injury would be mediated , at least in part , through the increased ratio of Bcl 2 to Bax protein after ischemia reperfusion . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tg betaCTF99 / B6 mouse brain at 12 16 months showed severely down regulated calbindin , phospho CREB , and Bcl xL expression and up regulated phospho JNK , Bcl 2 , and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , it downregulated the expression of Bcl 2 and poly ( adenosine diphosphate ribose ) polymerase , but upregulated the expression of caspase 3 and Bax in dopaminergic ( SK N SH ) neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This BEA induced apoptosis in human NSCLC A 549 cells was also accompanied by the up regulation of Bax , Bak , and p Bad and down regulation of p Bcl 2 , but no effect on the levels of Bcl 10 ( L ) or Bad proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PBN pre treated cells showed significant inhibition of apoptotic features such as activation of caspase 3 , up regulation of Bax and p 53 , and down regulation of Bcl 2 compared to control cells upon exposure to ionizing radiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of pro apoptotic ( p 53 , p 21 , bax , bak and fas ) and anti apoptotic ( bcl 2 and bcl 10 ) proteins in serous versus mucinous borderline ovarian tumours . ^^^ MATERIALS AND METHODS : Immunohistochemical expression of pro apoptotic ( p 53 , p 21 , bax , bak , fas ) and anti apoptotic proteins ( bcl 2 , bcl 10 ) was determined in 34 borderline ( 19 mucinous , 15 serous ) , 20 benign ( 10 mucinous , 10 serous ) and 28 malignant ovarian tumours ( 9 mucinous , 19 serous ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , p 53 , phospho p 53 ( Ser 15 ) , and other apoptosis related proteins such as Bax , Bcl 2 , Bcl xL , pro caspase 3 , and pro caspase 9 were determined by Western blot analysis . ^^^ An increase in expression of the pro apoptotic factor Bax and a decrease in expression of the anti apoptotic factor Bcl 2 were also observed in a time dependent manner after exposure of 50 microM curcumin , while the expression of the anti apoptotic factor Bcl xL was unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DNA damage dependent on bcl 2 but not bax in head and neck squamous cell carcinoma lines . ^^^ CONCLUSION : We concluded that the p 53 apoptotic response to DNA damage was dependent on bcl 2 but not bax in head and neck SCC lines . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of progesterone on the expression of bax and bcl 2 and on caspase activity in bovine luteal cells . ^^^ The expression of bcl 2 and bax and level of active caspase 3 in cells was measured . ^^^ Moreover , P 4 decreased ( P < 0 . 05 ) while staurosporine increased ( P < 0 . 01 ) the ratio of bax / bcl 2 at both stages of the cycle . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of the mice with etanercept significantly reduced the degree of 1 ) spinal cord inflammation and tissue injury ( histological score ) ; 2 ) neutrophil infiltration ( myeloperoxidase evaluation ) ; 3 ) inducible nitric oxide synthase , nitrotyrosine , cyclooxygenase 2 , and cytokines expression ( TNF alpha and interleukin 1beta ) ; and 4 ) apoptosis ( terminal deoxynucleotidyl transferase dUTP nick end labeling staining and Bax and Bcl 2 expression ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A mitochondrial permeability transition pore activator significantly abolished the anti apoptotic effect of nicorandil in endothelial cells , indicating that the mechanism of protective effect of nicorandil is involved in the mitochondrial apoptotic pathway although it affects neither Bcl 2 nor Bax protein expression levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
T 2 toxin induces apoptosis , and selenium partly blocks , T 2 toxin induced apoptosis in chondrocytes through modulation of the Bax / Bcl 2 ratio . ^^^ In the present study , we have investigated the apoptotic effects of T 2 toxin on chondrocytes and the relationship between T 2 toxin induced chondrocyte apoptosis and its influence on Bcl 2 / Bax protein and mRNA expression . ^^^ The apoptosis induced by T 2 toxin involved an increased Bax / Bcl 2 ratio . ^^^ Bcl 2 mRNA expression remained unchanged in chondrocyte apoptosis induced by T 2 toxin treatment , while Bax mRNA expression increased following treatment with T 2 toxin . ^^^ Selenium could partly block the apoptosis of chondrocytes induced by T 2 toxin through decreasing the Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , chalcone also triggered the mitochondrial apoptotic signaling by increasing the amount of Bax and Bak and reducing the level of Bcl 2 and Bcl 10 ( L ) , and subsequently activated caspase 9 in MCF 7 and MDA MB 231 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : To determine whether an imbalanced interaction between proapototic and antiapoptotic signals may account for the loss of the normal cell growth control in benign prostatic hyperplasia ( BPH ) , the expression of some apoptosis regulating genes ( bcl 2 , bax , c myc , fas ) was investigated . ^^^ A positive relationship between the expression of bcl 2 and that of the two proapoptotic genes bax and fas was observed ( p < 0 . 05 , Spearman ' s rank correlation test ) , as well as between c myc and fas levels ( p < 0 . 005 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis indicated that the induction of apoptosis by GA and ursolic acid was accompanied with an activation of caspase 8 and a reduction in the anti apoptotic proteins , Bcl 2 and Bcl xL , although the pro apoptotic proteins , Bax and Bak , remained unaffected . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that apoptosis was induced by an increased expression of Bax and decreased expression of Bcl 2 after treatment with Ritonavir and ionizing radiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Abnormal expression of bcl 2 and bax in rat tongue mucosa during the development of squamous cell carcinoma induced by 4 nitroquinoline 1 oxide . 4 Nitroquinoline 1 oxide ( 4NQO ) induced rat tongue carcinogenesis is a useful model for studying oral squamous cell carcinoma . ^^^ The aim of this study was to investigate the expression of bcl 2 and bax during tongue carcinogenesis induced by 4NQO . ^^^ Although no histological changes were induced in the epithelium after 4 weeks of carcinogen exposure , bcl 2 and bax were over expressed ( P < 0 . 01 ) in all layers of the ' normal ' epithelium . ^^^ The expression levels were the same in all layers of epithelium for both the antibodies used ( bcl 2 or bax ) . ^^^ In dysplastic lesions at 12 weeks following carcinogen administration , the levels of bcl 2 and bax expression did not increase when compared to negative control with the immunoreactivity for bcl 2 being restricted to the superficial layer of epithelium . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , inhibition of PI 3 kinase did not alter levels of Bax , Bcl 2 , Bcl 10 ( L ) or Bim proteins in mitochondria but caused translocation of the pro apoptotic protein Bad to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This was accompanied by significant TRAIL and decreased caspase 3 mRNA expression , whereas the expression of Bcl 2 family members was low ( Bcl xl ) or absent ( Bcl 2 , Bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The cRL fraction up regulated the pro apoptotic genes p 53 and Bax and induced caspase 3 activation , and down regulated the pro survival gene Bcl 2 in both the lung cancer cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This occurred in the setting of increased Bax and cleaved poly ( ADP ribose ) polymerase , as well as decreased Bcl 2 and pAkt . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proximal tubular cells treated with cisplatin and bezafibrate were subjected to sub cellular fractionation and the presence of Bax , Bcl 2 , cytochrome c , and active caspase 3 in the cytosolic and mitochondrial membrane fractions was determined by Western blot analysis . ^^^ Bezafibrate treatment of LLC PK 1 cells prevented cisplatin induced translocation of proapoptotic Bax from the cytosol to the mitochondrial fraction , and increased the expression of antiapoptotic molecule Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis revealed the down regulation of Bcl 2 and Bcl xL and the up regulation of Bax and Bad by cerebral I / R insult . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also found that Myc reduces the expression of Bcl 2 and Bcl xL and that the apoptosis inducing stimuli up regulate Bax expression . ^^^ These results suggest that up regulation of mtCLIC , together with a reduction in Bcl 2 and Bcl xL , sensitizes Myc expressing cells to the proapoptotic action of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
While the expression of Bax , Bak , and Bcl 2 was comparable to the wild type cells , the selected clones showed specific up regulation of Bcl XL , an anti apoptotic protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVES : Thus , in the present study , we studied the effects of systemically administered Epo on antiapoptotic ( bcl 2 , bcl XL ) , proapoptotic ( bax and DP 5 ) gene expression following hypoxic ischemic brain injury in neonatal rats . ^^^ Bcl 2 and bax protein expression were also analyzed with immunohistochemistry . ^^^ Bcl 2 , bcl XL , bax , and DP 5 mRNA expression were analyzed by RT PCR . ^^^ Modulating effects of Epo on bcl 2 and bax protein expression were also revealed by immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We studied 82 human breast cancer samples from patients treated with neoadjuvant doxorubicin based chemotherapy and studied whether or not nuclear location of the transcription factor NF kappaB was associated with expression of bcl 2 and bax and whether or not expression of these proteins correlated with chemotherapy response . ^^^ A dedicated breast cancer pathologist who was unaware of the clinical outcome data dichotomized the slides as positive or negative based on the presence or absence of cytoplasmic staining for bcl 2 and bax or nuclear staining for NF kappaB . ^^^ RESULTS : Sixty one percent of the tumors were positive for bcl 2 , 85 % were positive for bax , and 16 % were positive for NF kappaB . ^^^ All bcl 2 positive tumors were also bax positive ( P < 0 . 0001 ) and all NF kappaB positive tumors were both bcl 2 positive ( P = 0 . 001 ) and bax positive ( P = 0 . 113 ) . ^^^ The pCR rates were NF kappaB positive 0 % ( 0 of 13 ) versus NF kappaB negative 13 % ( 11 of 69 ; P = 0 . 130 ) ; bcl 2 positive 4 % ( 2 of 49 ) versus bcl 2 negative 27 % ( 9 of 33 ; P = 0 . 004 ) ; and bax positive 6 % ( 4 of 69 ) versus bax negative 58 % ( 7 of 12 ; P < 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , A 1254 significantly increased the expression of the pro apoptotic gene , Bax , concomitantly reducing the level of the anti apoptotic gene , Bcl 2 , in the cumulus cell compartment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TGF beta 1 induction of apoptosis was associated with augmented expression of Bax , a pro apoptotic member of the Bax / Bcl 2 family , inhibition of Bcl 2 , an anti apoptotic member of the same family , and inhibition of both cIAP 1 and XIAP , two inhibitors of the caspase cascade . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ileal tissue samples were taken for detection of bax and bcl 2 gene expression using RT PCR technique . ^^^ Enterocyte apoptosis increased significantly after bowel resection in jejunum and ileum compared to sham animals and was accompanied by an increased bax gene expression and a decreased bcl 2 gene expression in ileal samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This permeabilization of the mitochondrial outer membrane depends on activation and oligomerization of multidomain Bcl 2 family proteins Bax or Bak . ^^^ Although specific members of the Bcl 2 family can activate these proapoptotic proteins , we found that heat directly activated Bax or Bak to induce cytochrome c release . ^^^ Our results support an emerging paradigm , wherein the activation of Bax or Bak and the blockade of antiapoptotic Bcl 2 proteins are pivotal steps in the mitochondrial pathway of apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of adenine nucleotide translocator , voltage anion dependent channel , Bax , Bcl 2 , Bcl xL , AIF and Smac / Diablo , were similar in both cell lines , whereas cytochrome c content was divided by three in the resistant cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The study aimed at the analysis of polymorphisms in the gene coding for the nicotinic acetylcholine receptor alpha 4 subunit ( CHRNA 4 ) and the evaluation of the extent of the oxidative damage to DNA ( 8 oxo2dG ) , as well as the level of proteins participating in DNA repair ( p 53 , PARP ) and DNA degradation ( Bax : Bcl 2 , 85 kDa fragment ) in the peripheral blood lymphocytes of the patients suffering from Alzheimer ' s disease ( AD ) and in the healthy individuals of the control group . ^^^ In the AD patients the increased levels of oxidized guanine were demonstrated in DNA , accompanied by the elevated expression of p 53 , Bax , PARP , and of a 85 kDa protein subunit as well as an augmented ratio of Bax : Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Immunohistochemistry was applied to 134 paraffin embedded specimens of UC for the detection of COX 2 , p 53 , bcl 2 , caspase 3 , bax protein , MLH 1 and hTERT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS : The effect of salidroside on peripheral blood cells , BMCs , and bone marrow cell cycle in bone marrow depressed anemia mice was detected by automatic blood cell analysator , white blood count and flow cytometry ( FCM ) respectively , and the expression of Bcl 2 and Bax of BMCs was detected by immunohistochemistry method simultaneously . ^^^ Additionally , the expression of Bcl 2 in BMCs was increased in low dose and high dose salidroside groups , especially the increase was significant in the low dose salidroside group ; moreover , the expression of Bax in BMCs was reduced significantly in both low dose and high dose salidroside groups . ^^^ CONCLUSION : These data suggest that salidroside may promote the recovery of hematopoietic function of the bone marrow depressed anemia in mice by ending off G0 / G1 phase arrest , accelerating G0 / G1 S phase and S G2 / M phase transition , up regulating Bcl 2 expression , down regulating Bax expression , and inhibiting BMCs apoptosis . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bistability in apoptosis : roles of bax , bcl 2 , and mitochondrial permeability transition pores . ^^^ We examine the role of Bax and Bcl 2 synthesis and degradation rates , as well as the number of mitochondrial permeability transition pores ( MPTPs ) , on the cell response to apoptotic stimuli . ^^^ We show that high expression rates of Bcl 2 can counteract the effects of Bax . ^^^ This study supports our contention , based on mathematical modeling , that cooperativity in apoptosome formation is critically important for determining the healthy responses to apoptotic stimuli , and helps define the roles of Bax , Bcl 2 , and MPTP vis  vis apoptosome formation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chronic treatment of lithium , in agreement with the delayed onset of mood stabilizing effects of lithium , up regulates cell survival molecules ( e . g . , Bcl 2 , cyclic AMP responsive element binding protein , brain derived neurotrophic factor , Grp 78 , Hsp 70 , and beta catenin ) , while down regulating pro apoptotic activities ( e . g . , excitotoxicity , p 53 , Bax , caspase , cytochrome c release , beta amyloid peptide production , and tau hyperphosphorylation ) , thus preventing or even reversing neuronal cell death and neurogenesis retardation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Subsequent studies revealed the expression of MGr 1 Ag , P glycoprotein ( P gp ) , MDR associated protein ( MRP ) , Bcl 2 and Bax in gastric cancer cells via Western blot analysis . ^^^ Western blot analysis suggested that MGr 1 Ag may regulate the expression of P gp , MRP , Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical evaluation showed that CS treatment produced an increase in the cellular levels of Bax , t Bid , cleaved caspase 3 , phospho p 53 , phospho JNK , and FasL but a decline in Bcl 2 and Mcl 1 ( p < 0 . 001 for all ) in rat terminal bronchioles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It has been shown that the novel antiapoptotic protein Bax inhibitor 1 ( BI 1 ) represents a new type of regulator of cell death pathways controlled by Bcl 2 and Bax . ^^^ METHODS : Surgically resected lung specimens were obtained from 32 patients with peripheral adenocarcinomas , and BI 1 gene expression was examined and compared with expression of the p 53 , bcl 2 and Bax genes . ^^^ Eleven of 32 tumors ( 34 . 4 % ) were positive for the p 53 protein , only 1 of 32 tumors ( 3 . 1 % ) was positive for the Bcl 2 protein , and 26 of 32 tumors ( 81 . 3 % ) were positive for the Bax protein . ^^^ Protein expressions of p 53 , Bcl 2 , and Bax , as detected by immunohistochemistry , were not associated with BI 1 gene expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results suggest that the protective effects of rosiglitazone on acetaldehyde induced apoptosis may be ascribed to ability to induce the expression of anti oxidant enzymes and to regulate Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of caspase mRNA and the ratio of Bax to Bcl 2 were evaluated by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Bax / Bcl 2 in the culture group was significantly lower than in the overnight group ( 3 . 2 + / 0 . 66 versus 8 . 1 + / 0 . 95 , P < 0 . 05 ) , suggesting that apoptosis had been already destined early after isolation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of pro survival genes bcl 2 and bcl xl was down regulated by 10 % to 60 % ; expression of the proapoptosis gene bax was up regulated by 23 % to 85 % . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PCP induced reference memory impairment , and a decrease of the ratio of an anti apoptotic Bcl 2 family member ( Bcl XL ) to a pro apoptotic analogue ( Bax ) in the posterior cingulate cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These protective effects are related to its ability to attenuate oxidative stress , regulate the expression of apoptotic proteins Bcl 2 , Bax , P 53 , and caspase 3 , protect mitochondria , upregulate nerve growth factor and its receptors , and interfere with amyloid precursor protein metabolism . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Activated STAT5a / b / T cells showed increased expression of proapoptotic ( caspases , DNA repair genes , TNF / TNFR associated factor family genes ) and decreased antiapoptotic mRNAs in microarrays , while Western blots confirmed reduced antiapoptotic Bcl 2 and elevated proapoptotic Bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we investigated the polarization of T cell subsets , the expressions of apoptotic proteins Fas / FasL on the subsets and the level of anti apoptotic gene bcl 2 and bax mRNA . ^^^ With increasing level of bcl 2 mRNA and decreasing expression of bax mRNA in ITP children , the ratio of bcl 2 / bax mRNA was improved obviously , which was positive correlated with the ratio of Th1 / Th2 . ^^^ This polarization pattern of T cell subsets might be related to the high ratio of bcl 2 / bax mRNA and the abnormal expressions of Fas and FasL on T cell subsets . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PC reversed I / R induced activation of p 38 MAPK , Bax , and caspase 3 , suppression of Bcl 2 , and increase in TdT mediated dUTP nick end label positive apoptotic cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A decrease in expression of Bcl 2 , Bcl 10 ( L ) and pro caspase 3 was observed after exposure to 40 microM curcumin , while the levels of p 53 and Bax were increased in the curcumin treated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytotrophoblasts also expressed a 2 fold higher level of p 53 , a 2 fold lower level of 60 kDa Mdm 2 protein , a 2 fold higher level of Bak , but no differences in the expression of 90 kDa Mdm 2 , Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , Bad , and Bad phosphorylated at the serine ( 112 ) , serine ( 136 ) , or serine ( 155 ) sites , compared to the syncytiotrophoblasts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we studied the expression of the apoptosis related proteins p 53 , bcl 2 , bax , p 21 and fas in proliferative ( n=9 ) and secretory ( n=9 ) endometrium , and in peritoneal ( n=11 ) , ovarian ( n=20 ) and colorectal ( n=20 ) endometriosis , by qualitative and semi quantitative immunohistochemical methods using the percentage of positive cells and HSCORE analysis . ^^^ In endometrium , p 53 , p 21 and fas expression was low , whereas bax and bcl 2 expression was elevated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we show that NMDA and K 25 mediated neuroprotection is associated with an increase in the levels of Bcl 2 , an inhibition of K 5 mediated increase in Bax , and the inhibition of the release of apoptogenic factors from mitochondria such as Smac / DIABLO and cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our data demonstrated that the treatment with all the tea ingredients resulted in ( 1 ) significant inhibition in growth of implanted prostate tumors , ( 2 ) reduction in the level of serum prostate specific antigen , ( 3 ) induction of apoptosis accompanied with upregulation in Bax and decrease in Bcl 2 proteins , and ( 4 ) decrease in the levels of VEGF protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study was designed to estimated the effects of B cell lymphoma / leukemia 2 ( Bcl 2 ) , Bcl 2 associated protein 10 ( Bax ) , and osteopontin ( OPN ) on the apoptosis of renal tubular cells induced by NaF at different levels . ^^^ The technique of reverse transcription polymerase chain reaction and densitometer scanning volume density were used to evaluate the changes of Bcl 2 , Bax , and OPN mRNA in tubular cells treated with different doses of NaF ( 0 , 1 , 5 , 7 . 5 , 12 . 5 mgF / L ) for 48 h . ^^^ Compared to control , the level of Bax mRNA significantly increased at cells of the 7 . 5 and 12 . 5 mg F / L groups and the expression of Bcl 2 mRNA obviously decreased at cells of the 5 and 7 . 5 mg F / L groups . ^^^ The results suggested that NaF induces the apoptosis in renal tubules via activation of the Bax expression and Bcl 2 suppression ; OPN probably acts as protective role against apoptosis in fluoride treated renal cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of this study was to investigate whether apoptosis may contribute to the pathogenesis of epidermal changes in dermatitis herpetiformis ( DH ) and , in particular , whether certain apoptosis related markers such as Bax , Bcl 2 , Fas and Fas ligand ( FasL ) take part in this process . ^^^ Skin lesions from six and perilesional skin from four DH patients were stained with monoclonal antibodies to Bax , Bcl 2 , Fas and FasL . ^^^ In our immunohistochemical analysis , Bax / Bcl 2 ratio was almost the same in the epidermis of perilesional / lesional DH , BP and healthy skin specimens . ^^^ In DH and BP specimens both Bax and Bcl 2 proteins were increased in the dermal perivascular compartment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Regression analysis showed that increases in aortic superoxide anion ( O . 2 ) with aging were significantly correlated with changes in the expression and / or regulation of proteins involved in metabolic ( AMPK alpha ) , signaling ( mitogen activated protein kinases ( MAPKs ) along with c Src ) , apoptotic ( Bax , Bcl 2 , Traf 2 ) and transcriptional ( NF kappaB ) activities . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The down regulation of the cyclin A , Bcl 2 , and Bcl 10 ( L ) expression with the simultaneous up regulation of the p 21 and Bax expression , and caspase 9 activation was observed in ECSC after bufalin treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of DNA PKcs , Ku70 / 80 , Rad 51 and Rad 54 genes that related to DNA damage repair , and Bcl 2 and NF kappaB genes that related to antiapoptosis , were up regulated , but the expression of proapototic Bax gene was down regulated in the radioresistant cells as compared to each parental counterpart . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After treatment with IDN 5109 , Bcl 2 and Bcl XL were down regulated , Bax was up regulated , and caspase 3 was activated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Eupatilin induced the apoptosis of AGS cells as revealed by a decrease in the ratio of pro apoptotic Bax and anti apoptotic Bcl 2 , as well as the cleavage of caspase 3 and poly ( ADP ribose ) polymerase ( PARP ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MCTF treatment activated caspase 8 , 9 and 3 , and led to cleaved poly ( ADP ribose ) polymerase and release of cytochrome c into cytosol in a concentration dependent manner , while MCTF did not affect Bax or Bcl 2 protein levels as shown by Western blot analysis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The pro apoptotic Bcl 2 family proteins , Bak , Bax , and Bim have been shown to be required for disruption of mitochondria and intrinsic cell death of self reactive B cells whereas the anti apoptotic Bcl 2 , Bcl xL , and Mcl 1 can prevent cell death by interfering with the action of Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The G ( 0 ) / G ( 1 ) phase arrest and apoptosis were associated with disappearance of mitochondrial cytochrome c and increased expression of Bax but decreased expression of Bcl 2 proteins in the inhibited cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , Western blots were done to study the relative expression of Bcl 2 and Bax in the tumors . ^^^ In tumors of mice treated with single modality therapies , Bax to Bcl 2 ratios decreased significantly after castration , whereas this ratio remained high after docetaxel treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although , the A . camphorata induced apoptosis was associated with Bax protein levels , negligible Bcl 2 reduction was observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Silymarin pre treatment reversed the effect of UV irradiation on the expression of phosphorylated Akt and phosphorylated p 53 ( regulated by Akt activation ) , followed by down regulation of Bax and up regulated expressions of Bcl 2 and Bcl xL proteins in UV irradiated A 375 S2 cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of melittin also potently induced proapoptotic protein p 53 , Bax , and caspase 3 expression but decreased antiapoptotic protein Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl 2 expression after permanent focal ischemia in rats . ^^^ We investigated a possible effect of MH and a combination of CE and MH ( CE + MH ) on the changes of infarction size , DNA fragmentation , and immunoreactivities for Bcl 2 and Bax after 24 h of permanent middle cerebral artery occlusion ( MCAO ) in rats . ^^^ Immunoreactivities for Bcl 2 and Bax were stained . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Correlation between Bcl 2 and Bax in atrophic and hypertrophic type of actinic keratosis . ^^^ We have analysed the expression of apoptosis related proteins TP 53 , Bcl 2 and Bax in 30 atrophic and 30 hypertrophic AK cases . ^^^ Monoclonal antibody for TP 53 and Bcl 2 and polyclonal antibody for Bax ( DAKO , Copenhagen , Denmark ) were used . ^^^ RESULTS : Expression of TP 53 showed no significant differences between two analysed groups ( chi 2 test , P = 0 . 35636 ) whereas expression of Bcl 2 and Bax protein was significantly higher in atrophic compared to hypertrophic AK ( chi 2 test , P = 0 . 01458 and P = 0 . 00358 , respectively ) . ^^^ Comparison of Bcl 2 : Bax ratio in two analysed AK showed significantly higher value in hypertrophic compared to atrophic AK ( Mann Whitney U test , P = 0 . 02272 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Surprisingly , apoptosis , as well as the localization of the pro apoptotic Bcl 2 family member , Bax , was optimal in marmosets during the mid secretory phase when plasma progesterone levels were high . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
There was no increase in labeling or change in localization of labeling that distinguished between these cases for active caspase 3 , 8 , 9 , Bax , Bcl 2 or TRADD . ^^^ Bax , Bcl 2 and TRADD mRNA levels also differed little between case types , although there were small but significant decreases in Bax mRNA levels in AD compared to control cases and Bcl 2 mRNA in AD cases compared to pathologically aged and control cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , AE was associated with a marked increase in Fas / APO1 receptor and Bax expression but it inhibited Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and Bid , two proapoptotic Bcl 2 family members , inhibit homologous recombination , independently of apoptosis regulation . ^^^ In order to analyse the relationships between regulation of apoptosis and homologous recombination ( HR ) , we overexpressed proapoptotic Bax or only BH 3 Bid proteins or antiapoptotic Bcl 2 or Bcl XL , in hamster CHO cells or in SV 40 transformed human fibroblasts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In the study of the molecular mechanism of this phenomenon , it was found that GnTV AS reduced the expressions of anti apoptotic proteins , such as phosphorylated protein kinase B and phosphorylated Bad as well as Bcl 2 and Bcl 10 ( L ) , and elevated those of pro apoptotic proteins , including Bax , full length caspase 3 and its activated fragments as well as anti oncoprotein p 53 . ^^^ The increased active caspase 3 was the consequence of the elevated Bax / Bcl 2 ( Bcl 10 ( L ) ) activity ratio in the cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The myocardial ultrastructure was examined , and the content of ATP , phosphocreatine , and glycogen in myocardium , the extent of myocyte apoptosis , and the amount of Bcl 2 and Bax products were determined after 120 min ischemia assessed by TUNEL and immunocytochemistry . ^^^ RESULTS : There was no significant difference between the reserpinized hearts and the NS control group with respect to heart function , myocardial ultrastructure , ATP , phosphocreatine , or glycogen content , myocyte apoptosis , or amount of Bax or Bcl 2 products . ^^^ However , relative to the normal saline group , in the norepinephrine treated hearts , heart function , and myocardial ultrastructure deteriorated significantly , apoptosis and amount of Bax product increased significantly , and the ATP , phosphocreatine , and glycogen content decreased significantly , as did the amount of Bcl 2 product . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Osteoblast and osteocyte apoptosis associated with androgen action in bone : requirement of increased Bax / Bcl 2 ratio . ^^^ To explore mechanism , the apoptosis regulators Bcl 2 ( antiapoptotic ) and Bax ( proapoptotic ) were evaluated . ^^^ Western analysis revealed that DHT decreased Bcl 2 resulting in a significantly increased Bax / Bcl 2 ratio . ^^^ Increased Bax / Bcl 2 ratio was essential since either Bcl 2 overexpression or Bax downregulation by RNA interference ( RNAi ) partially abrogated or reversed DHT enhanced osteoblastic apoptosis . ^^^ Thus , although estrogen can be antiapoptotic , androgen stimulates osteoblast and osteocyte apoptosis through an increased Bax / Bcl 2 ratio even in anabolic settings . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Angiotensin 2 elevated p 38 mitogen activated protein kinase ( MAPK ) activity , decreased Bcl 2 protein , and increased Bax protein and caspase 3 activity . ^^^ The effect of apocynin on apoptosis was associated with reduced p 38 MAPK activity , increased Bcl 2 protein , and decreased Bax protein and caspase 3 activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax regulation of corneal homeostasis in genetically altered mice . ^^^ PURPOSE : The antiapoptotic protein Bcl 2 and its proapoptotic counterpart Bax may play an important role in the regulation of corneal epithelial renewal . ^^^ To understand the physiologic importance of Bcl 2 and Bax in the healthy cornea , epithelial and stromal thickness , proliferation , and surface cell exfoliation rates were examined in the central cornea of genetically altered mice overexpressing Bcl 2 and lacking Bax . ^^^ METHODS : Adult Bcl 2 transgenic ( n = 55 ) and Bax knockout ( n = 82 ) mice and wild type controls were clinically prescreened at no less than 2 months of age for any ocular developmental abnormalities . ^^^ RESULTS : No significant changes in proliferation or cell death were found in either group compared to wild type littermate controls ; however , epithelial and stromal thicknesses were greater in the Bcl 2 transgenic group compared to wild type and decreased in the Bax knockout . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was accompanied by cytochrome c release to the cytoplasm and Bax translocation to the mitochondria , while the levels of the anti apoptotic proteins Bcl 2 and Bcl xL remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis induced by each TM was associated with a significant ( approximately 400 % ) increase in caspase 8 activity , but no change in caspase 9 activity , and Western analyses revealed a marked up regulation of Fas ( approximately 500 % ) and FADD ( approximately 300 % ) with no change in expression of Bax , Bcl 2 , or Bcl xL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The gastric blood flow was measured by H 2 gas clearance method ; the plasma gastrin and gastric luminal somatostatin were determined by RIA and expression of cyclooxygenase ( COX ) 2 and apoptotic Bax and Bcl 2 proteins were evaluated by Western blot . ^^^ The H . pylori infection was accompanied by the fall in gastric blood flow , the marked increase in plasma gastrin , the significant fall in gastric somatostatin levels and Bcl 2 protein expression , and the rise in expression of COX 2 and Bax proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mitochondrial damage was caused by the translocation of Bax but not other pro apoptotic Bcl 2 family proteins to the mitochondria . ^^^ The status of Bcl 2 and Bax may influence the sensitivity of tumors to this combination and thus can be a target of further therapeutic intervention . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
No correlation was found between expression levels of topoisomerase 2 , Pgp , Bcl 2 , Bax , or Bad and decreased sensitivity of Daudi cells to VP 16 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Eighteen HNPCC related endometrial cancers , each matched by tumour type , stage and grade with two sporadic endometrial cancers , were examined for proliferation , apoptosis and the expression of oestrogen and progesterone receptors , cyclin B 1 , D 3 and E , p 21 , p 27 , bcl 2 , bax , p 53 and COX 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
After treatment with oridonin for 48 hr , the percentage of disruption of delta psi m gradually increased in a dose dependent manner along with marked changes of cell apoptosis , and necrotic cells increased remarkably after the cells were treated with oridonin for 72 hr ; Western blotting showed cleavage of the caspase 3 zymogen protein ( 32 kDa ) with the appearance of its 20 kDa subunit when apoptosis occurred ; expression of Bcl 2 and Bcl XL was downregulated remarkably while expression of Bax and Bid was upregulated concurrently after the cells were treated with oridonin for 24 hr . ^^^ We therefore conclude that oridonin has significant antiproliferation effects on HPB ALL cells by induction of apoptosis as well as directly causing cell necrosis and that oridonin induced apoptosis on HPB ALL cells is mainly related to the disruption of delta psi m and activation of caspase 3 as well as downregulation of anti apoptotic protein Bcl 2 , Bcl XL , and upregulation of pro apoptotic proteins Bax and Bid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results indicated that garlic extract , when administered to the colo 205 cell cultures , reduced the percetange of viable cells , induced apoptosis , increased the levels of Bax , cytochrome c and caspase 3 , but decreased the level of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bcl 2 , Bax , and caspase 3 proteins in mouse hippocampus were studied by immunohistochemical staining . ^^^ Bax and caspase 3 positive cells increased while Bcl 2 positive cells decreased remarkably . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consistent with accelerated apoptosis in PKC delta transfectants , compared to EV cells , PKC delta upregulation increased proapoptotic regulator Bax two fold at mRNA and protein levels , while antiapoptotic Bcl 2 protein was decreased by 50 % at a post transcriptional level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Surprisingly , the effect of TSA on the proapoptotic Bcl 2 proteins was mixed , the two isoforms of PUMA ( alpha , beta ) , Noxa , Bax were downregulated , while Bim was upregulated . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Upon exposure to heat shock , NtBHA pre treated cells showed significant inhibition of apoptotic features such as activation of caspase 3 , up regulation of Bax , and down regulation of Bcl 2 compared to untreated cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The relative resistance of PC 3 cells to drug induced apoptosis was associated with constitutive Akt activation , higher levels of cFLIP L and Bcl 2 , and lower levels of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was identified by sub G 0 cell population , and upregulation of Bax , downregulation of Bcl 2 , release of Ca2+ , decreased the mitochondrial membrane potential and then led to the release of mitochondrial cytochrome C into the cytoplasm and caused the activation of caspase 3 , and finally led to the occurrence of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR analyses demonstrated an increased level of HO 1 , Mn SOD , Cox 2 , iNOS , TNFalpha , TNFR 1 , IL 1beta , IL 6 , Bax , Bak , and Bad gene expression , while Bcl 2 mRNA expression level decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the effect of prostaglandin ( PG ) E 1 on hypoxia / re oxygenation ( H / R ) apoptosis and the expression of bcl 2 and bax in cultured neonatal rat cardiomyocytes . ^^^ The expression of bcl 2 and bax was detected by in situ hybridization and immunohistochemical staining . ^^^ The results of in situ hybridization and immunohistochemical staining showed that the bcl 2 content in the H / R group was lower than that in the control group ; bax content showed the reverse . ^^^ Prostaglandin E 1 obviously has anti apoptotic effects on cardiomyocytes and the mechanisms probably involve the inhibition of bax expression and increased expression of bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis is mediated by the activation of caspases 3 and 8 , up regulation of the apoptotic gene bax with concomitant down regulation of the anti apoptotic gene bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bid , BH 3 interacting domain death agonist , was initially cloned based in its ability to interact with both Bcl 2 and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 , Bid , and Bad inhibit assembly sub stoichiometrically , whereas peptides from Bak and Bax promote tubulin polymerization at near stoichiometric concentrations . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This response was also markedly attenuated by coexpression with the Bax antagonist antiapoptotic protein Bcl 2 , confirming a role for Bax activation in this apoptotic response . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protective effects of kallikrein , through the kinin B 2 receptor , were accompanied by increased cerebral nitric oxide and Bcl 2 levels , Akt phosphorylation , and reduced NAD ( P ) H oxidase activity , superoxide production , Bax levels , and caspase 3 activity . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 but not Bax has a prognostic significance in tongue carcinoma . ^^^ Apoptosis regulatory genes include the antiapoptotic bcl 2 gene and the proapoptotic bax gene . ^^^ The aim of this study was to determine the spectrum of Bax and Bcl 2 expression , and to correlate these findings with clinicopathologic variables and prognosis . ^^^ METHODS : In this study we have evaluated the immunohistochemical expression of Bcl 2 and Bax proteins in a series of 35 squamous cell carcinomas of the tongue . ^^^ RESULTS : Immunoreactivity for Bax was detected in 37 . 1 % and for Bcl 2 in 8 . 6 % of cells , and for both proteins the staining was cytoplasmic and granular . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis in SW 872 human liposarcoma cells induced by quercetin was mediated through the activation of caspase 3 , Bax , and Bak and then cleavage of PARP and downregulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AIMS AND BACKGROUND : To evaluate the function and distribution of BAG 1 protein in hyperplastic and neoplastic prostate tissue and establish the relationship between this protein and BCL related proteins ( BCL 2 and BAX ) , androgen receptor ( AR ) expression and chromogranin A . ^^^ BAG 1 , BCL 2 , BAX , androgen receptor and chromogranin A immunostaining was performed by means of standard avidin biotin peroxidase methods . ^^^ In the hyperplasia group there was a negative correlation between BAX and BCL 2 , and between AR and M 30 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increase in apoptosis was associated with a dose dependent up regulation of cytosolic factor , such as Bax and release of cytochrome c , and down regulation of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Another glial activation related gene ( CTSS ) was slightly up regulated in obex , whereas constitutive expression was detected for SOD 1 , YWHAZ , PRNP , and the apoptosis related genes BCL 2 , MCL 1 , and BAX . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exercise training attenuates age induced elevation in Bax / Bcl 2 ratio , apoptosis , and remodeling in the rat heart . ^^^ We tested the hypothesis that 12 wk of exercise training would attenuate age induced increases in remodeling , apoptosis , and Bax / Bcl 2 ratio in rat left ventricle . ^^^ Further , exercise training in the aging reduced caspase 9 levels and Bax / Bcl 2 ratio by lowering Bax protein expression while increasing Bcl 2 levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Estrous cycle dependent changes in expression and distribution of Fas , Fas ligand , Bcl 2 , Bax , and pro and active caspase 3 in the rat ovary . ^^^ Fas , Fas ligand , Bcl 2 , Bax and caspase 3 mRNA expression and proteins were detected in all ovarian tissue extracts , though the amount of protein varied with the phase of the estrous cycle . ^^^ The proportion of luteal cells that stained positively for Fas , Bax and caspase 3 increased with the age of the corpus luteum , while Fas ligand and Bcl 2 immunostaining was strongest in newly formed corpora lutea and decreased thereafter . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It also reversed the potassium cyanide induced increased expression of Bax and decreased expression of Bcl 2 to control levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Embryos also showed widespread expression of both the bcl 2 and Bax gene products , which are both associated with cell death pathways . ^^^ TNF alpha , synthesized and secreted by the embryo itself , may be able to exert a paracrine effect on the incidence of cell death in tissues of the embryo , and the cell death process may be related to the expression of bcl 2 and Bax gene products . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To determine whether this phenomenon results from altered expression of Bcl 2 or related proteins , Northern and Western analysis was employed to assess the effects of bryostatin 1 and other PKC activators on steady state levels of Bcl 2 , Bax , Bcl 10 , and Mcl 1 mRNA and protein . ^^^ These effects were accompanied by unaltered expression of Bcl 2 , Bax , and Bcl 10 ( L ) , and by a further increase in Mcl 1 protein levels . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The analyzed genes were bax , bcl 2 and bcl 10 , with known responses to genotoxic stress ( bcl 2 ) or ionising radiation ( bax and bcl 10 ) in MCF 7 human breast cancer cells . ^^^ The results obtained indicate that the cell line studied expresses the mentioned genes and they demonstrate that irradiation with low radon doses of MCF 7 cells induces underexpression of both bax and bcl 2 genes . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The immunoblot study also showed that the glucan induced depletion of the antiapoptotic Bcl 2 protein , but not the proapoptotic Bax protein , so that the Bax / Bcl 2 ratio was elevated in the breast cancer cells at the time when the most prominent apoptosis was also observed . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RT PCR revealed that Ad Asc myc downregulated expression of bcl 2 and upregulated expression of Bax , and no appreciable changes were observed in the expression of E2F 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Exposure of HUVEC to IR did not change the level of Bax and Bcl 2 and did not cause activation of caspase 3 , indicating that radioprotection by lovastatin does not depend on the modulation of the mitochondrial death pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of BMS 345541 on tumor cell growth was through mitochondria mediated apoptosis , based on the release of apoptosis inducing factor , dissipation of mitochondrial membrane potential , and reduced ratio of B cell lymphoma gene 2 ( Bcl 2 ) / Bcl associated 10 protein ( Bax ) in mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Levels of proapoptotic factors ( bim and bax ) increased and levels of antiapoptotic factors ( bcl 2 and bcl xL ) decreased in a dose dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunofluorescence analysis indicates that ( DIPP L Leu ) 2 L LysOCH 3 induced upregulation of pro apoptotic Bax and downregulation of anti apoptotic Bcl 2 and Bcl 10 ( L ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Increase in cyclic AMP and decrease in TNF alpha levels were identified in the ipsilateral cortex under treatment with cilostazol accompanied by decreased Bax formation and cytochrome c release with increased Bcl 2 production in the penumbral area as well as in the in vitro human umbilical endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We sought to test the hypothesis that epidermal growth factor receptor signaling regulates specific Bcl 2 family members ( Bax and Bcl w ) to direct apoptosis and adaptation after massive small bowel resection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Alzheimer ' s amyloid beta peptide ( 1 42 ) induces cell death in human neuroblastoma via bax / bcl 2 ratio increase : an intriguing role for methionine 35 . ^^^ These results support the hypothesis that Abeta induced neurotoxicity occurs via bax over expression , bcl 2 down regulation , and caspase 3 activation , first indicating that methionine 35 redox state may alter this cell death pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Surprisingly , there was no differential expression of important proteins like Bcl 10 ( L ) , Bcl 2 or Bax that might explain enhanced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of Bcl 2 and Bax , the changes of mitochondria membrane potential ( MMP ) , cytochrome c release and activation of caspase 3 were also investigated in both cell lines . ^^^ Western blotting also showed that berberine increased the levels of Bax and cytochrome c and decreased the levels of Bcl 2 in both cell lines . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here we show that peptides including any of the two alpha helix fragments of the hairpin of Bcl 2 associated protein 10 ( Bax ) can independently induce release of large labelled dextrans from synthetic lipid vesicles . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These events were associated with alterations in the levels of Bax and Bcl 2 shifting the Bax : Bcl 2 ratio in favor of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Up regulation and intracellular redistribution of Bcl 2 associated 10 protein ( Bax ) , a pro apoptotic Bcl 2 family protein , were observed in DC treated with these compounds and represent a suitable mechanism leading to activation of the intrinsic apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analysis showed that GW 8510 downregulated the expression of 10 linked inhibitor of apoptosis ( XIAP ) but had no detectable effect on the expression of Bax , Bak , or Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study examined the mechanisms involved in free fatty acid ( FFA ) induced lysosomal permeabilization and the role of Bax , a Bcl 2 family member , in this event . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further mechanistic analysis demonstrate that CTX 3 induced the loss of mitochondrial membrane potential ( Dym ) , cytochrome c release from mitochondria into the cytosol and activation of capase 9 , caspase 3 , as well as markedly enhancing the expression of Bax , but not Bcl 2 , protein in the cells . ^^^ Taken together , these results suggest that CTX 3 may induce apoptosis through a mitochondrial and caspase dependent mechanism and alteration of Bax / Bcl 2 ratio in human colorectal Colo 205 cancer cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Here , we found that radiation induced apoptosis in NSCs via the mitochondrial pathway , upregulating the ratio of Bax to Bcl 2 and releasing cytochrome c into the cytoplasm . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hepatic mRNA levels were measured for several pro apoptotic adaptors / regulators , including FasL , Fas receptor , FADD , TRADD , Bad , Bak , Bax , and Bcl 10 ( S ) , and anti apoptotic regulators , including Bcl w , Bcl 10 ( L ) , Bcl 2 , and Bfl 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Panduratin A treatment to cells was found to result in inhibition of procaspases 9 , 8 , 6 and 3 with significant increase in the ratio of Bax : Bcl 2 , suggesting the involvement of a mitochondrial dependent apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
All tested thimidine containing PS ODN , irrespective of their primary sequences , reduced the expression of Bcl 2 protein and increased the levels of the proapoptotic molecules p 53 , Bid , Bax in CLL cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altered Bcl 2 and Bax expression is associated with cultured first trimester human cytotrophoblasts apoptosis induced by hypoxia . ^^^ We therefore investigated the effect of hypoxia on the apoptosis of cultured first trimester human cytotrophoblasts and the expression of apoptosis relevant proteins , Bcl 2 and Bax . ^^^ Cellular apoptosis was monitored by TUNEL and Annexin 5 binding , and the expression of Bcl 2 and Bax was determined by Western blot analysis . ^^^ Apoptosis increased significantly in cytotrophoblasts cultured for 24 h under hypoxic conditions in contrast with those cultured under standard conditions , meanwhile expression of Bcl 2 reduced , and that of Bax increased . ^^^ These changes suggested that hypoxia induced apoptosis in cultured first trimester cytotrophoblasts with altered Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting analysis revealed that clove infusion upregulates the expression of pro apoptotic proteins p 53 and Bax , and downregulates the expression of anti apoptotic protein Bcl 2 in the precancerous stages . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
As a result of the changed p 53 activity , expression of the downstream apoptosis related genes such as bcl 2 and bax is accordingly altered and the apoptotic events are either largely abrogated or enhanced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also determined the mRNA expression of heat shock protein ( HSP ) 70 , caspase 3 , bcl 2 , and bax using a quantitative reverse transcription polymerase chain reaction method . ^^^ The ratio of bcl 2 / bax expression tended to increase in the normal liver but decrease in the fatty liver following heat stress . ^^^ CONCLUSIONS : The detection of apoptosis , together with changes in the mRNA expression of HSP 70 and the expression ratio bcl 2 / bax mRNA may indicate vulnerability of a fatty liver to heat stress and may support the hypothesis that morphologic change is induced in a fatty liver by exposure to heat stress . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , p 53 features in PADI 4 induced apoptosis by increasing intracellular p 21 to control cell cycle and by Bax accumulation to decline Bcl 2 function , destroy delta psi ( m ) , release cytochrome c to cytoplasm and activate the caspase cascade . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These kinases also mediated the increase of Bax levels , but only in some conditions Bcl 2 depletion caused by the peptide . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The hemodynamic parameters { mean arterial pressure ( MAP ) , heart rate ( HR ) , left ventricular end diastolic pressure ( LVEDP ) , left ventricular peak positive ( + ) LVdP / dt ( rate of pressure development ) and negative ( ) LVdP / dt ( rate of pressure decline ) } were monitored at pre set points throughout the experimental duration and subsequently , the animals were sacrificed for immunohistopathological ( Bax , Bcl 2 protein expression & TUNEL positivity ) and histopathological studies . ^^^ RESULTS : Chronic treatment with Cl significantly reduced TUNEL positivity ( p < 0 . 05 ) , Bax protein ( p < 0 . 001 ) and upregulated Bcl 2 ( p < 0 . 001 ) expression in comparison to control IR group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , through inhibiting phosphorylation of Bcl 2 ( a cytosolic target of JNK ) and the release of Bax from Bcl 2 / Bax dimers , Tat JBD attenuated Bax translocation to mitochondria and the release of cytochrome c induced by ischemia / reperfusion . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These responses on UVB and / or sanguinarine treatments were associated with ( a ) decrease in Bcl 2 and Bcl 10 ( L ) and ( b ) increase in Bax , Bid , and Bak protein levels . ^^^ Bax knockdown and Bcl 2 overexpression resulted in a rescue of HaCaT cells from sanguinarine mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Collectively the data indicate that saturated FFAs induce JNK dependent hepatocyte lipoapoptosis by activating the proapoptotic Bcl 2 proteins Bim and Bax , which trigger the mitochondrial apoptotic pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also show evidence suggesting that IAN 4 and IAN 5 are associated with anti apoptotic proteins Bcl 2 and Bcl xL , whereas IAN 1 is associated with pro apoptotic Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax levels were higher ( approximately 2 folds ) than monolayers , while the ratio ( Bcl / Bax ) remained unaltered . ^^^ CONCLUSION : Development of S negative cells and reduced endogenous and radiation induced ROS coupled with higher levels of anti ( Bcl 2 ) as well as pro ( Bax ) apoptotic regulators observed in spheroids suggest the intricate / complex nature of endogenous as well as induced stress resistance that could exist in tumors , which contribute to the treatment resistance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The small organic compound HA 14 1 prevents Bcl 2 interaction with Bax to sensitize malignant glioma cells to induction of cell death . ^^^ A functional imbalance between proapoptotic Bax and antiapoptotic Bcl 2 is likely to participate in the resistance of cancer cells to therapy . ^^^ This sensitizing effect is lost if Bcl 2 expression , but not Bcl xL expression , is knocked down or if cells only express a mutant of Bax that does not interact with Bcl 2 . ^^^ This points to a specific Bcl 2 inhibitory function of HA 14 1 and implies that it selectively involves hindrance of Bcl 2 binding to Bax , which HA 14 1 inhibits in cell free assays and in cells in receipt of an apoptotic stimulation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Chondrocyte apoptosis and expression of Bcl 2 , Bax , Fas , and iNOS in articular cartilage in patients with Kashin Beck disease . ^^^ We investigated apoptotic chondrocyte death and the expression of Bcl 2 , Bax , Fas , and inducible nitric oxide synthase ( iNOS ) in articular cartilage from patients with KBD in order to determine the pathogenesis of chondronecrosis in KBD . ^^^ KBD patients were diagnosed according to `` Pathological Criteria to Diagnose KBD in China . ' ' Chondrocyte apoptosis was detected by TUNEL staining , and Bcl 2 , Bax , Fas , and iNOS positive articular chondrocytes were stained by immunohistochemistry . ^^^ Articular cartilage was classified in 3 zones , and positive findings were counted by light microscopy for cytoplasmic staining by polyclonal antibodies of Bcl 2 , Bax , Fas , and iNOS and apoptotic chondrocytes by TUNEL . ^^^ CONCLUSION : The apoptotic chondrocytes and Bcl 2 , Bax , Fas , and iNOS positive chondrocytes were significantly more numerous in patients with KBD than in controls . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neuroprotective effects of ebselen are associated with the regulation of Bcl 2 and Bax proteins in cultured mouse cortical neurons . ^^^ In this study , we sought to determine the relationship between alterations in the expression of Bcl 2 and Bax proteins and intracellular levels of calcium and the protective effects of ebselen with a concentration range of 0 . 01 20 microM against glutamate toxicity in cultured mouse cortical neurons . ^^^ Pretreatment with ebselen ( 8 microM ) also prevented apoptotic alterations , completely reversed the suppression of Bcl 2 expression , and significantly inhibited Bax overexpression , but did not alter elevated intracellular concentrations of calcium induced by glutamate . ^^^ These results indicate that the neuroprotective effects of ebselen at low doses are associated with the regulation of Bcl 2 and Bax proteins but appear to be independent of glutamate mediated elevation of intracellular calcium , suggesting that different mechanisms are involved in the actions of low and high dose regimens . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study investigated induction of apoptosis in NB 1 oral carcinoma cells by paclitaxel and the expression of Bcl 2 and Bax in relation to this apoptotic cell death . ^^^ The present results suggest that paclitaxel can induce apoptosis of NB 1 cells , which may be mediated by down regulation of Bcl 2 together with up regulation of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The survival period , lymphocytes apoptosis , expressions of Bcl 2 / Bax proteins in lymphocytes , and histopathological findings were then compared among these groups . ^^^ On the seventh post transplant day , the apoptosis index ( AI ) of lymphocytes in portal area and the positive rate of Bax protein in lymphocytes in RES C group were significantly increased in comparison with those in control group ( both P < 0 . 05 ) , whereas there is no obvious difference in the expression of Bcl 2 protein in lymphocytes between the control group and various drug groups ( all P < 0 . 05 ) , and a histological examination revealed apparent difference in the severity of rejection between the RES C group and control group ( P < 0 . 05 ) . ^^^ Inducing apoptosis of lymphocytes and upregulating the ratio of Bax / bcl 2 proteins in lymphocytes in allograft liver may be part of the mechanisms . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The decreased ratio of Bcl 2 to Bax suggests that apoptosis is initiated by the intrinsic mitochondrial pathway through the release of caspase dependent and independent apoptogenic molecules . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , Bax expression increased together with p 53 and p 21 ( WAF1 / CIP1 ) , while the Bcl 2 expression decreased in the immortalized and malignant keratinocytes treated with DFO . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Although inhibition of the cell cycle and mitosis is not necessarily dependent on the tumor ' s p 53 status , the expression of p 21 , GADD 45 and apoptosis related genes ( BAX , BCL 2 ) are regulated by wt p 53 , in addition to their regulation by TGF beta or MDA 7 in mutated p 53 tumors . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
SIV infection in PK 15 and HeLa cells was shown to decrease the cellular levels of Bcl 2 protein compared to that of mock infected control cells at 24 h post infection , whereas expression levels of Bax protein increased in the PK 15 cells , but did not increase in HeLa cells by SIV infection . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We also examined the regulation of BCL 2 and BAX protein expression in the population of CD138+ plasmocytes . ^^^ In most patients , the BCL 2 / BAX ratio was lower in cell cultures supplemented with mixture of lovastatin and thalidomide in comparison with cell cultures supplemented with lovastatin or thalidomide alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypoxia also significantly increased Bax and decreased Bcl 2 mRNA and protein expression , thereby increasing Bax / Bcl 2 ratio . ^^^ Moreover , nicorandil significantly inhibited the hypoxia induced changes in Bax and Bcl 2 expression , and concomitant increased Bax and decreased Bcl 2 immunoreactivity in mitochondria . ^^^ These effects of nicorandil in Bax and Bcl 2 expression were significantly blunted by cotreatment of ODQ and 5 HD , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Loss of expression of proapoptotic Bcl 2 proteins , namely Bax and Bak , in primary melanomas is associated with a worse long term prognosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Altogether , our results emphasize translocation of Bcl 2 family proteins to have central regulatory functions of UV induced apoptosis in melanocytes and suggest cathepsins to be proapoptotic mediators operating upstream of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study , we demonstrated that NAPS induces apoptosis of Jurkat cells by activating Bak , but not Bax , which are both members of a proapoptotic subfamily of the Bcl 2 proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expressions of Bcl 2 and Bax proteins were examined by streptavidin biotin peroxidase method . ^^^ The decreased neuron AI and the expressions of up regulated Bcl 2 protein and down regulated Bax protein were also observed in the ginkgo leaf extracts treated group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Apoptotic proteins in tumors that showed positive staining were the highest for Bax ( 99 % ) , Fas ( 92 % ) , FasL ( 87 % ) , Rb ( 87 % ) , p 21 ( WAF 1 ) ( 73 % ) , and p 53 ( 70 % ) , and the lowest for c myc ( 58 % ) and Bcl 2 ( 58 % ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NVP AEW 541 treatment suppressed the expression of the antiapoptotic proteins Bcl 2 and survivin , while the expression of the proapoptotic protein BAX was stimulated in a dose dependent manner . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Pretreatment of the cells with PND or PNN for 24 h following 1mM SNP , an exogenous NO donor , exposure for 1h , resulted significantly in reduction of cell death induced by SNP determined by MTT assay , LDH release and Hoechst staining . 5 microM PND and PNN also reduced the up regulation of the pro apoptotic gene , Bax , down regulation of the anti apoptotic gene , Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , the anti apoptotic proteins Bcl 2 and Bcl 10 were down regulated , whereas pro apoptotic protein Bax remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Quantitative real time PCR studies demonstrated that prolonged palmitic acid supplementation induced down regulation of the anti apoptotic Bcl 2 mRNA levels ( 22 % ) and up regulation of the pro apoptotic Bax mRNA levels ( 300 % ) , leading to disruption of the pro / anti apoptotic balance ( Bax / Bcl2=3 ) . ^^^ In conclusion , prolonged exposure to saturated palmitic acid induces lipoapoptosis in exocrine pancreatic AR42J cells , through disturbance of the Bax / Bcl 2 balance . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Neural cell apoptosis was determined by terminal deoxynucleotidyl t ransferase ( TdT ) mediated dUTP fluorescence nick end labeling ( TUNEL ) method and the expression of bcl 2 and bax were examined histologically in the spinal cord with immunohistochemistry . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment with 0 . 4 mmol / L H ( 2 ) O ( 2 ) up regulated Bax but down regulated Bcl 2 in a time dependent manner , while Bcl xL expression remained unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These results suggest that AP 2alpha induces apoptosis by down regulating Bcl 2 and utilizing a bax / cytochrome c / Apaf1 / caspase 9 dependent mitochondrial pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Mitochondrial bioenergetics and permeability transition pore plays a crucial role in this process , although several redox sensitive genes , proteins and transcription factors , such as Bcl 2 , Bax , nuclear factor kappa B , regulate the decision making power of mitochondria , which have the final authority to decide whether a cell lives or dies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To assess the probable mechanism ( s ) of altered apoptosis , oxidative stress parameters , caspase 3 activity , and expression of Bcl 2 and Bax were determined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
This study was to investigate the expression and prognostic significance of representative apoptosis related oncogenes ( Survivin , Bcl 2 , Bax , and Fas ) in stage 1 2 NSCLC . ^^^ METHODS : The expression of Survivin , Bcl 2 , Bax , and Fas in 115 specimens of stage 1 2 NSCLC and 20 specimens of non tumor lung tissue were detected by SP immunohistochemistry . ^^^ RESULTS : The positive rates of Survivin and Bcl 2 were significantly higher in NSCLC than in non tumor lung tissues ( 62 . 61 % vs . 10 . 00 % , P < 0 . 001 ; 49 . 57 % vs . 15 . 00 % , P < 0 . 05 ) ; the positive rates of Bax and Fas were significantly lower in NSCLC than in non tumor lung tissues ( 31 . 30 % vs . 65 . 00 % , P < 0 . 05 ; 46 . 96 % vs . 80 . 00 % , P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ponicidin treatment of lung cancer cells caused downregulation of anti apoptotic protein Bcl 2 and survivin as well as upregulaton of pro apoptotic protein Bax in a time dependent manner when apoptosis ocurred . ^^^ We therefore conclude that ponicidin has significant apoptosis inducing effects by activation of caspase 3 8 , and 9 as well as downregulation of anti apoptotic protein Bcl 2 , survivin and upregulation of pro apoptotic protein Bax , with caspase 8 acting as an upstream activator . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The effect of CT treatment on the `` in vitro ' ' clonogenicity , the Population Doubling Time ( PDT ) , apoptosis , PKA activation and Bax and Bcl 2 expression was evaluated in WEHI 3B cell line and its CT resistant subclone ( WEHI 3B / CTRES ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Fasting induced moderate apoptosis in liver tissue , as evidenced by increased levels of transforming growth factor beta 1 ( TGF beta 1 ) and Bax proteins and by a dramatic drop in the level of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Death promoting Bcl 2 family members , such as Bax , can promote cytochrome c release and fragmentation of the mitochondrial network , whereas apoptosis inhibitory members , such as Bcl 2 and Bcl xL , can antagonize these events . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , the results showed that FME induced apoptosis was accompanied by up regulation of Bax and Bad , and down regulation of Bcl 2 and Bcl XL . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Utilizing an in vivo rat model data is provided indicating that preconditioning completely prevents cardiac ischemia / reperfusion induced : ( 1 ) loss in the activity of the redox sensitive Krebs cycle enzyme alpha ketoglutarate dehydrogenase ; ( 2 ) declines in NADH linked ADP dependent mitochondrial respiration ; ( 3 ) insertion of the pro apoptotic Bcl 2 protein Bax into the mitochondrial membrane ; and ( 4 ) release of cytochrome c into the cytosol . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Our results indicated that combined treatment led to an increased apoptotic cell death in U 937 cells , which is correlated with the phosphorylation of the 5 Jun sarcoma virus 17 oncogene homolog ( c JUN ) NH ( 2 ) terminal kinase protein ( JNK ) , the activation of caspases , the increase in B cell leukemia / lymphoma 2 ( Bcl 2 ) associated 10 protein ( Bax ) , the decrease in Bcl xL protein ( Bcl XL ) levels , the loss of mitochondria membrane potential and the release of cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Akt phosphorylation , Bax , Bcl 2 , Bad , and phosphorylated Bad ( phospho Bad ) expression was assessed by immunoblotting . ^^^ Akt phosphorylation and Bcl 2 and phospho Bad expression increased after isoflurane preconditioning , whereas Bax expression decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hippocampus bcl 2 and bax expression and neuronal apoptosis after moderate hypothermic cardiopulmonary bypass in rats . ^^^ At 1 h after CPB , in 6 rats per group , hippocampus was processed for the apoptotic gene ( bcl 2 and bax ) messenger RNAs detection by reverse transcriptase polymerase chain reaction , and messenger RNA expression was determined by the ratio of the polymerase chain reaction product of bcl 2 or bax to the beta actin gene . ^^^ At 6 h after CPB , in 6 rats per group , hippocampus expression of Bcl 2 and bax protein was determined by immunohistochemistry , and neuronal apoptosis was detected by TUNEL . ^^^ Group 1 had increased ratios of bcl 2 / beta actin , bax / beta actin , and bax / bcl 2 mRNA at 1 h after CPB ( bcl 2 / beta actin , 0 . 82 + / 0 . 14 versus 0 . 63 + / 0 . 07 ; P = 0 . 03 ; bax / beta actin , 1 . 04 + / 0 . 14 versus 0 . 56 + / 0 . 03 ; P = 0 . 00 ; bax / bcl 2 , 1 . 31 + / 0 . 12 versus 0 . 84 + / 0 . 09 ; P = 0 . 02 ; Group 1 versus Group 2 , respectively ) . ^^^ Group 1 had increased bcl 2 and bax protein expression in hippocampal CA 1 region at 6 h after CPB ( bcl 2 , 0 . 18 + / 0 . 05 versus 0 . 09 + / 0 . 01 ; P = 0 . 02 ; bax , 0 . 20 + / 0 . 06 versus 0 . 04 + / 0 . 02 ; P = 0 . 01 ; Group 1 versus Group 2 , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in biochemical parameters , the content of glutathione ( GSH ) and the activity of superoxide dismutase ( SOD ) in liver tissue , Bcl 2 and Bax protein expression and morphological changes of liver tissue were compared between both groups . ^^^ One and 24 h after reperfusion , the positive expression rate of Bcl 2 protein was higher in group G than in group C ( P < 0 . 05 ) and the positive expression rate of Bax protein was lower in group G than in group C ( P < 0 . 05 ) . ^^^ CONCLUSION : Our results suggest that Ala Gln pretreatment provides the rat liver with significant tolerance to warm ischemia reperfusion injury , which may be mediated partially by enhancing GSH content and regulating the expression of Bcl 2 and Bax proteins in the liver tissue . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results suggest that the protective effects of adiponectin on MPP+ induced apoptosis may be ascribed to its anti oxidative properties , anti apoptotic activity via inducing expression of SOD and catalase , and regulation of Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemistry was employed to examine the expression of the antiapoptotic proteins Bcl 2 and Bcl XL and the proapoptotic proteins Bad , Bak , Bax , Bid , Bim , and p 53 in 21 cases of gastric cancer . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In this study we elaborate on the immune response and mechanisms of persistence of mycobacteria in lesions by analysing , using immunohistochemistry , the expression of cytokines [ tumour necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , transforming growth factor beta ( TGF beta ) and interferon gamma ( IFN gamma ) ] , apoptotic cells and apoptosis related proteins [ Bcl 2 , Bax , Fas ligand ( FasL ) and Fas ] in the human tuberculous lymphadenitis lesions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein expressions of Cbfa 1 , Bax , Bcl 2 , caspase 3 , and caspase 9 , cytochrome C and TNF alpha were determined by Western immunoblot . ^^^ RESULTS : After the transient transfection with the two isforms of Cbfa 1 , MBA 1 , the cells apoptotic rates increased , and the ratio of Bax / Bcl 2 , the expressions of cytochrome C , caspase 3 , caspase 9 , and TNF alpha were significantly increased . ^^^ Bax / Bcl 2 , cytochrome C , caspase 9 , caspase 3 , and TNF alpha are also involved in the apoptosis pathway . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of yizhi jiannao granule concentration fluid ( YCF ) on the behavior , the apoptosis rate of hippocampus neuron and the expression of apoptosis gene Bcl 2 , Bax in senescence accelerated mice Senile Prone / 8 ( SAMP / 8 ) , and to discuss some mechanism of traditional chinese medicine YCF in improving the capability of learning and memory . ^^^ Four groups were given different drugs for 8 weeks , their behavior changes were observed , and the hippocampus were taken out to examine the apotosis rate by flow cytomeutry ( FCM ) and the expression of Bcl 2 , Bax mRNA by RT PCR . ^^^ RESULTS : In the YCF group , the escape latency was significantly shortened , the time of swim in the platform quadrant significantly increased , the apoptosis rate of hippocampus neural decreased ; the level of Bcl 2 mRNA and the rate of Bcl 2 / Bax increased , and the level of Bax mRNA decreased . ^^^ CONCLUSION : Yizhi jiannao granule can decrease the neuron apoptosis rate and the Bax level , increase the Bcl 2 level , and modulate the rate of Bcl 2 / Bax in SAMP / 8 brain , which is probably part of the mechanisms of inhibiting the apoptosis and improving learning and memory . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The efficiency of p 53 gene therapy was evaluated by following tumor growth and expression of P 53 related downstream proteins ( P 21 , MDM 2 , Bcl 2 , Bax ) . ^^^ PCI mediated p 53 gene transfer led to higher P 53 protein expression that was correlated with induction of Bax and P 21 proapoptotic proteins , repression of Bcl 2 as well as activation of caspase 3 , and cleavage of PARP . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of the pro apoptotic Bax gene and anti apoptotic Bcl 2 and Glut 1 genes in fibroblast derived blastocysts was significantly higher than in cumulus cell derived and in vitro fertilized blastocysts . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Auto activation of the apoptosis protein Bax increases mitochondrial membrane permeability and is inhibited by Bcl 2 . ^^^ The interactions between BH 3 region only proteins and multi BH region proteins such as Bax and Bcl 2 have been proposed to be the dominant interactions required for initiating apoptosis . ^^^ Bcl 2 inhibited the membrane permeabilizing activity of peptide activated Bax . ^^^ Bcl 2 inhibited Bax auto activation . ^^^ We therefore propose that Bax auto activation amplifies the initial death signal produced by BH 3 only proteins and that Bcl 2 functions as an inhibitor of Bax auto activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Triphlorethol A reduced the expression of bax and activated caspase 3 induced by radiation , but recovered the expression of bcl 2 decreased by radiation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Changes in the ratio of Bax and Bcl 2 mRNA expression and their cellular localization throughout the ovulatory cycle in the human oviduct . ^^^ PURPOSE : To examine changes in the ratio of Bax and Bcl 2 mRNA expression throughout the ovulatory cycle in the ampullary region of the human oviduct . ^^^ METHODS : The mucosal layer was isolated from the human oviduct tissue and semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) analysis of mRNA of Bax and Bcl 2 was performed . ^^^ Immunohistochemistry provided the cellular localization of the Bax and Bcl 2 proteins . ^^^ The ratio of expression of Bax and Bcl 2 mRNA was examined in the ampullary region of the oviduct in samples obtained in the follicular , periovulatory , and luteal phases of the ovulatory cycle . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein levels of Bcl 2 and cyclooxygenase 2 were significantly decreased , but Bax levels were increased in both of the CLA isomer groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The authors tried to determine the responsible molecules ; however , expression of TRAIL receptors ; pro caspase 3 / 8 / 9 ; Fas associated death domain protein ( FADD ) ; tumor necrosis factor receptor 1 associated death domain protein ( TRADD ) ; silencer of death domain ( SODD ) ; FLICE inhibitory protein ( FLIP ) ; and Bcl 2 , Bcl xL , and Bax in FLS was not modulated by IFN gamma . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax inducers or Bcl 2 inhibitors ) , and purinoceptor P ( 2Y2 ) antagonists to inhibit mucin secretion or P ( 2Y2 ) agonists to hydrate secretions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further study proved that the apoptosis related protein Bcl 2 was upregulated whereas p 53 and Bax were downregulated in the PrP ( C ) transfected cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax was mainly expressed in spermatocytes and spermatids and Bcl 2 in basal germ cells ( immunohistochemistry ) . ^^^ CONCLUSIONS : Our results demonstrated that extrinsic , mitochondrial and possibly ER pathways are inducers of germ cell apoptosis in EAO and that Bax and Bcl 2 proteins modulate this process . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Involvement of Bax , Bcl 2 and NF kappaB were examined by RT PCR and EMSA . ^^^ Culture of cells in the presence of 25 mM D glucose ( 1 ) enhanced apoptosis stimulated by serum depletion , ( 2 ) enhanced activation of caspase 3 , ( 3 ) inhibited NF kappaB activation , and ( 4 ) decreased Bcl 2 : Bax ratio . ^^^ Inhibition of NF kappaB using SN 50 , also increased mesangial cell apoptosis , and decreased Bcl 2 : Bax ratio . ^^^ Addition of TGF beta 1 to mesangial cells mimicked the effect of high glucose reducing NF kappaB expression and Bcl 2 : Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further analysis indicated an alteration in the ratio of Bax / Bcl 2 proteins in favor of apoptosis , and the knockdown of Bax using Bax siRNA transfection of 4T1 cells resulted in blocking of GSPs induced apoptosis . ^^^ Dietary GSPs ( 0 . 2 and 0 . 5 % , w / w ) significantly inhibited the growth of the implanted 4T1 tumor cells and increased the ratio of Bax : Bcl 2 proteins , cytochrome c release , induction of Apaf 1 and activation of caspase 3 in the tumor microenvironment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Among proapoptotic proteins , Bax expression was activated in H ( 2 ) S treated cells but not Bid , and the antiapoptotic proteins Bcl 10 ( L ) and Bcl 2 did not show any activation in pancreatic acinar cell apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In ischemic cerebral cortex , the level of Bcl 2 was decreased , and the level of Bax was significantly increased . ^^^ Estradiol prevents the injury induced decrease of Bcl 2 and increase of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blot analyses showed an increase in activities of calpain and caspase 12 , upregulation of Bax : Bcl 2 ratio , release of cytochrome c from mitochondria , and increase in caspase 9 and caspase 3 activities during apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of the mice with 4 benzyl 2 methyl 1 , 2 , 4 thiadiazolidine 3 , 5 dione ( TDZD 8 ) , a potent and selective GSK 3beta inhibitor , significantly reduced the degree of 1 ) spinal cord inflammation and tissue injury ( histological score ) ; 2 ) neutrophil infiltration ( myeloperoxidase activity ) ; 3 ) inducible nitric oxide synthase , nitrotyrosine , and cyclooxygenase 2 expression ; and 4 ) and apoptosis ( terminal deoxynucleotidyl transferase dUTP nick end labeling staining and Bax and Bcl 2 expression ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was characterized by activation of the apoptotic key enzyme , caspase 3 , as well as by detection of changes in the expression of the pro and anti apoptotic proteins , BAX and Bcl 2 , after NVP AEW 541 treatment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western Blotting was used to detect Bcl 2 , Bax and caspase 3 protein expressiom . ^^^ Accordingly , Bax protein expression had no change and caspase 3 protein expression showed significantly be up regulated ; ( 2 ) MTT results showed that Bcl 2 siRNA transfectants had higher cell inhibitory rates after treated with 5 FU or HCPT ; ( 3 ) flow cytometry results demonstrated that sub G 1 population increased in Bcl 2 siRNA transfected cells compared with negative siRNA or untreated cells . ^^^ After addition 5 FU ( 1300 mg / l ) and HCPT ( 0 . 72 mg / l ) , Bcl 2 siRNA cells showed higher sub G 1 population than negative siRNA or untreated cells . siRNA targeting Bcl 2 gene can specifically down regulate Bcl 2 expression , increased Bax / Bcl 2 ratio expression and caspase 3 activity in HepG 2 cells , which lead to increase cells spontaneous apoptosis and sensitize cells to 5 FU or HCPT . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Gene expression analysis using RT PCR indicates the significant down regulation of Bcl 2 and up regulation of Bax expression in liver of malaria infected mice suggesting the involvement of mitochondrial pathway of apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
ALK 7 ca induced the expression of proapoptotic factor Bax but suppressed the expression of antiapoptotic factors Bcl 2 , Bcl XL , and Xiap . ^^^ Thus , ALK 7 induced apoptosis is at least in part through two Smad dependent pathways , Bax / Bcl 2 and Xiap . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cryosections of retinas harvested at various postnatal periods were immunostained with antibodies against c Jun , Bcl 2 , and Bax . ^^^ Semiquantitative RT PCR analysis showed that mRNAs for c Jun and Bax were upregulated at P 21 and P 28 , but those for Bcl 2 were unchanged . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Treatment of T 24 cells with beta lapachone resulted in a down regulation of Bcl 2 expression and up regulation of Bax expression . beta lapachone induced apoptosis was also associated with activation of caspase 3 and caspase 9 , inhibition of IAP expression , and degradation of poly ( ADP ribose ) polymerase , phospholipase C gamma 1 and beta catenin proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of 3 aminobenzamide on Bcl 2 , Bax and AIF localization in hippocampal neurons altered by ischemia reperfusion injury . the immunocytochemical study . ^^^ In this study we investigated the effect of 3 AB on the localization and expression of apoptosis inducing factor ( AIF ) and on two proteins from Bcl 2 family : Bcl 2 and Bax in hippocampal area CA 1 , on the 4th day after 3 min of forebrain ischemia in gerbils . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results showed that the production of hydrogen peroxide derived from MnSOD dismutation activated caspase 8 , which might down regulate Bcl 2 expression and induce Bax translocation to mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the proapoptotic Bcl 2 family protein Bax was involved in cell death induction by hTERT siRNAs . ^^^ Inhibition of mitochondrial membrane permeabilization by overexpression of Bcl 2 or expression of the cytomegalovirus encoded protein vMIA ( viral mitochondrial inhibitor of apoptosis ) , which acts as a specific Bax inhibitor , prevented the induction of cell death by the combination of hTERT depletion and chemotherapeutic agents . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax , a multi domain protein belonging to the large family of Bcl 2 proteins , has a pivotal role for the initiation of the cytochrome c mediated apoptosis , a vital physiologic process to eliminate damaged or unwanted cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Induction of proliferating cell nuclear antigen ( PCNA ) , transforming growth factor beta ( TGFbeta ) , cyclo oxygenase 2 ( cox 2 ) , cpla 2 , osteocalcin ( OC ) , c myc , fibroblast growth factor 2 ( fgf 2 ) , bcl 2 , bax , and fgf 2 message as well as FGF 2 protein were significantly depressed in microg when compared to ground ( gr ) . ^^^ Artificial onboard gravity normalized the induction of c myc , cox 2 , TGFbeta , bax , bcl 2 , and fgf 2 message as well as FGF 2 protein synthesis in spaceflight samples . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of anti apoptotic Bcl 2 and pro apoptotic Bax proteins was visualized by western blot analysis . ^^^ Furthermore , greater Bcl 2 and attenuated Bax expression was found in the CY treated rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein expressions of Bcl XL , Bcl 2 , Bax , cytochrome c and AIF were studied by Western blotting . ^^^ This was accompanied by down regulation of Bcl XL and Bcl 2 and up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : This study was designed to investigate the relationship between apoptosis and Bcl 2 and Bax expressions in uterine cervical cancer after balloon occluded arterial infusion ( BOAI ) . ^^^ The expressions of Bcl 2 and Bax proteins were investigated by immunohistochemical staining . ^^^ Although Bcl 2 immuno reactivity was faintly detected , the expression of Bax increased at 3 days ( 49 . 4 + / 10 . 4 % ) after BOAI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In additional studies , treatment of A 431 cells with berberine ( 15 75 microM ) for 72 h resulted in a significant dose dependent increase in apoptosis ( 31 60 % , P < 0 . 05 0 . 001 ) than non berberine treated control ( 11 . 7 % ) , which was associated with an increased expression of pro apoptotic protein Bax , decreased expression of anti apoptotic proteins Bcl 2 and Bcl xl , disruption of mitochondrial membrane potential , and activation of caspases 9 , 3 and poly ( ADP ribose ) polymerase . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cases and controls were assessed for the presence of EBV by polymerase chain reaction ( PCR ) and in situ hybridization techniques , the presence of 30 base pair deletion of the LMP 1 ( del LMP 1 ) gene and for the expression of p 53 , Ki 67 , bcl 2 and Bax by immunohistochemistry . ^^^ Overexpression of Ki 67 , p 53 and bcl 2 was detected in 78 . 1 % , 62 . 5 % and 20 % of cases , whereas loss of Bax was detected in 18 % of the cases . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Molecular mechanism of cell death was investigated by p 53 , Bcl xL , Bcl 2 , Bax protein , activity of caspase 3 and 12 , and activity of telomerase . ^^^ CIZAR reduced expression of Bcl 2 and Bcl xL proteins but induced expression of Bax protein . ^^^ CIZAR ( R ) prevents the proliferation of OVCAR 3 cells by inactivation of m aconitase activity and induces apoptosis by induction of proapoptotic gene ( Bax ) , repression of antiapoptotic genes ( Bcl 2 , Bcl xL ) , and consequently activation of caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The role of PARP 1 , Bax , and Bcl 2 in apoptosis was analyzed by Western blotting . ^^^ Anti apoptotic Bcl 2 levels were suppressed while pro apoptotic Bax levels were enhanced . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Susceptibility to apoptosis was measured at the level of degradation product ( proportion of cells in the sub G 0 cell cycle fraction in which apoptotic bodies accumulate ) , pro apoptotic effector ( activated caspase 3 ) , and molecular regulators ( P 53 , Bax and Bcl 2 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of p 53 , p 21 ( WAF 1 ) , Bax , Bak , bcl 2 , as well as total and phosphorylated Cdc 2 in the absence and presence of olomoucine , a phosphorylated Cdc 2 kinase inhibitor , was then determined . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
AGEs markedly elevated Bax and decreased NF kappaB , but not Bcl 2 expression . ^^^ HGF significantly promoted the expression of Bcl 2 and NF kappaB , while decreasing the activities of caspase 3 and 9 without affecting Bax level . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TPL was also found to affect the expression levels of proteins involved in response to drug treatment ( e . g . , p 53 , bcl 2 , bax , p 21 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study examined whether activation of HL 60 derived neutrophils by C5a modulates the transcription of two members of the Bcl 2 family , Bax ( pro apoptotic ) and Bcl 2 ( anti apoptotic ) molecules , and whether the cAMP response element binding protein ( CREB ) transcription factor mediates these effects through the phosphatidylinositol 3 kinase ( PI3K ) / Akt and extra cellular signal regulated kinase ( ERK ) signalling pathways . ^^^ The cells were lysed and quantified for gene specific Bax and Bcl 2 mRNA . ^^^ The Bax : Bcl 2 mRNA ratio markedly decreased at 30 min ( P=0 . 028 ) . ^^^ The Bax : Bcl 2 mRNA ratio markedly increased in the presence of alkaline phosphatase ( P=0 . 046 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Consequently , high dose of Tiron led to apoptotic cell death , which showed the DNA fragmentation , the caspase activation and the unbalance between antiapoptotic ( Bcl 2 ) and proapoptotic proteins ( Bax ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The patterns of expression of the Bcl 2 , Bax , and Bcl xL proteins were examined immunocytochemically in rat hippocampus and neocortex after severe hypobaric hypoxia ( 180 Torr for 3 h ) and severe hypoxia preconditioned by intermittent mild hypoxia ( 360 Torr for 2 h daily , for 3 consecutive days , 24 h prior to severe hypoxia ) . ^^^ The preconditioning to severe hypoxia protected neurons from the posthypoxic apoptotic transformations , the up regulation of Bax expression , and resulted in persistent overexpression of Bcl 2 and Bcl xL . ^^^ We conclude that the protective action of hypoxic preconditioning is at least in part mediated by shifting of neuronal Bax / Bcl 2 Bcl xL ratio to a favor of antiapoptotic proteins Bcl 2 and Bcl xL . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hepatic cytosolic Bax , Bcl 2 , cytochrome c , and calpain mu expressions were measured by Western blot analysis . ^^^ Additionally , cytosolic caspase 3 activity and cytosolic fractions of Bax , Bcl 2 , cytochrome c , and calpain mu protein expressions were decreased in rats receiving CCl 4 plus S . miltiorrhiza extract compared with those receiving CCl 4 alone . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 changes conformation to inhibit Bax oligomerization . ^^^ In transfected cells and isolated mitochondria , Bcl 2 , but not the inactive point mutants Bcl 2 G145A and Bcl 2 V159D , undergoes a conformation change in the mitochondrial membrane in response to apoptotic agonists such as tBid and Bax . ^^^ Thus , Bcl 2 must change the conformation to inhibit tBid induced oligomerization of integral membrane Bax monomers and small oligomers . ^^^ The conformationally changed Bcl 2 sequesters the integral membrane form of Bax . ^^^ If Bax is in excess , apoptosis resumes as Bcl 2 is consumed by the conformational change and in complexes with Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated UPR signaling events in mice in the absence of the proapoptotic BCL 2 family members BAX and BAK [ double knockout ( DKO ) ] . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : Etoposide treatment ( 1 ) induced apoptosis in one clone , ES , but not in another clone , ER , ( 2 ) had no effect on the expression of the antiapoptotic proteins Bcl 2 and Bcl 10 ( L ) in both cell clones , whereas the proapoptotic proteins Bak and Bax were dramatically upregulated in ES , but not ER cells , and ( 3 ) induced more extensive processing of procaspase 8 , procaspase 9 , and the caspase 3 targeted substrates , topoisomerase 1 and PARP , in ES cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Surgical injury affected the expression of apoptosis related genes Bcl 2 , Bax , Bcl xL and Bcl xS , inducing a mean 3 . 5 fold decrease in the Bcl 2 / Bax ratio and a 9 fold decrease in the Bcl xL / S ratio 4 h after injury as compared with uninjured carotid arteries . ^^^ The imbalance between proliferative stimulus represented by surgery and the c myc mRNA decrease induced greater apoptosis in AS ODN treated carotid arteries without further affecting mRNA levels of Bcl 2 , Bax , Bcl xL and Bcl xS genes . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Urinary markers of exposure , Heat Shock Proteins ( HSPs ) 27 , 70 , 90 , 110 , immunologic biomarkers in peripheral blood lymphocytes : apoptosis , cell cycle analysis G 1 S G , typization of Natural Killer cells ( NK ) and receptors micronuclei ; frequency in peripheral blood lymphocytes and in exfoliated buccal mucosa cells ; activation ofspecific oncogenes ( bax , bcl 2 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The levels of Bax , a pro apoptotic member of Bcl 2 family , appeared increased at baseline in mast cells from diabetic rats compared with normal cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PAF increased the expression of mRNA and the protein synthesis of antiapoptotic factors , such as Bcl 2 and Bcl xL , but did not increase the expression of the proapoptotic factor , Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We investigated the role of the mitogen activated protein kinase ( MAPK ) signaling pathway ( ie , ERK1 / 2 ) and apoptosis regulating Bcl 2 family members ( ie , Bcl 10 ( L ) and Bax ) in the resolution of a rat carrageenan induced pleurisy model . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , through inhibiting phosphorylation of Bcl 2 and the release of Bax from Bcl 2 / Bax dimers , SP 600125 attenuated Bax translocation to mitochondria and the release of cytochrome c induced by ischemia / reperfusion ( I / R ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunoprecipitation coupled with immunoblot analysis further revealed a progressive increase in the complex formed between HSP 60 and mitochondrial or cytosolic Bax or mitochondrial Bcl 2 during endotoxaemia , alongside a dissociation of the cytosolic HSP 60 Bcl 2 complex . ^^^ We conclude that HSP 60 redistributed from mitochondrion to cytosol in the RVLM confers neuroprotection against fatal cardiovascular depression during endotoxaemia via reduced activation of the cytochrome c caspase 3 cascade of apoptotic signalling through enhanced interactions with mitochondrial or cytosolic Bax or Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Knockout of Bax or overexpression of Bcl 2 facilitated the survival of tetraploid cells at least as efficiently as the p 53 or p 21 knockout . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis mediated by the proapoptotic BCL 2 family members BCL 2 associated 10 protein ( BAX ) and BCL 2 antagonist / killer ( BAK ) is part of the antiviral response at the cellular level to limit virus replication . ^^^ These same 5 BCL 2 proteins cooperate with loss of retinoblastoma protein and p 53 tumor suppressor function , by inactivating the BAX and BAK apoptotic pathway to promote epithelial solid tumor growth and resistance to chemotherapy . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twelve weeks of treadmill exercise training increased anti apoptotic Bcl 2 , while markedly reducing DNA fragmentation , and cleaved caspase 3 , Bax , and Bax / Bcl 2 ratio in the white gastrocnemius and soleus muscles of old rats . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These changes were associated with elevation of oxidative stress , decreases of mitochondrial Bcl 2 and cytochrome c proteins , increases of cytosolic Bax and cytochrome c proteins , caspase 9 and caspase 3 activities and myocyte apoptosis , and downregulation of beta adrenergic receptor sensitivity and SR Ca2+ ATPase . ^^^ Combined treatment with vitamins C and E diminished oxidative stress , increased mitochondrial Bcl 2 protein , decreased cytosolic Bax , prevented cytochrome c release from mitochondria to cytosol , reduced caspase 9 and caspase 3 activities and myocyte apoptosis , blocked beta adrenergic receptor desensitization and SR Ca2+ ATPase downregulation , and attenuated LV dilation and dysfunction in HF after MI . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression of Bax , Bcl 2 , caspase 3 and caspase 9 was regulated differently with aging between muscle types and in a manner not consistent with mitochondria mediated apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , the cytosolic level of cytochrome c was increased together with a gradually down regulated Bcl 2 and up regulated Bax protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Interestingly , Bcl 2 / Bcl xL bispecific ASO treatment also resulted in the down regulation of Mcl 1 and up regulation of Bax . ^^^ Furthermore , the apoptotic induction by Bcl 2 / Bcl xL bispecific ASO was synergistically enhanced by siRNA mediated inhibition of clusterin , a cytoprotective chaperone that interacts with and inhibits activated Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
PI3K and Bcl 2 were down regulated whereas Bax expression was elevated in adipose tissue following IFN treatment as compared to adipose tissue of control DIO mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proapoptotic proteins Bax and Bak are required for MOMP , while the antiapoptotic Bcl 2 proteins , including Bcl 2 , Bcl xL , Mcl 1 , and others , prevent MOMP . ^^^ Different proapoptotic BH 3 only proteins act to interfere with the function of the antiapoptotic Bcl 2 members and / or activate Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Flow cytometry after Annexin labeling and PI staining were used to assess the apoptotic effects of Ang 1 on bEnd . 3 , and the expression of Bax / Bcl 2 , caspase 8 , caspase 3 , and Cyt C were detected with Western blotting and ELISA . ^^^ Ang 1 could decrease the expression of Bax , caspase 3 and 8 , and increase that of Bcl 2 . ^^^ The apoptosis was associated with decreased Bax expression , increased Bcl 2 expression , which result in Cyt C transferring from mitochondria to cytoplasm , and then caspases activation are reduced and cell apoptosis is suppressed . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Tumor sections were analyzed by immunohistochemistry for the expression of regulators of the cell cycle ( p 21 ; retinoblastoma protein ( pRb ) ) , of the intrinsic or extrinsic proapoptotic pathways ( p 53 ; murine double minute gene 2 protein ; tumour necrosis factor related apoptosis inducing ligand ( TRAIL ) R1 / DR4 ; TRAIL R2 / DR5 ) and of Bcl 2 related proteins ( Bcl 2 , Mcl 1 , Bax , Bak , Bok ) , which regulate the common mitochondrial apoptotic pathway . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Administration of higenamine ( bolus , i . p ) 1 h prior to I / R injury significantly decreased the release of cytochrome c , caspase 3 activity , and Bax expression but up regulated the expression of Bcl 2 , HO 1 , and HO enzyme activity in the left ventricles , which were inhibited by ZnPP 9 , an enzyme inhibitor of HO 1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and Bax in congenital naevi . ^^^ OBJECTIVES : The aim of this study was to investigate the expression of Bcl 2 anti apoptotic and Bax apoptotic proteins in congenital naevi in correlation with p 27 protein and Ki 67 proliferative index . ^^^ The immunohistochemical streptavidin biotin method was performed on paraffin sections for the detection of Bcl 2 ( cl100 / D5 ) , Bax ( cl2D2 ) , Ki 67 ( MIB 1 ) and p 27 ( 1B4 ) proteins with monoclonal antibodies . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To test the role of Bad , Bim , and other proapoptotic Bcl 2 family members in IL 3 withdrawal induced apoptosis , we generated IL 3 dependent cell lines from mice lacking the genes for Bad , Bim , Puma , both Bad and Bim , and both Bax and Bak . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis does not appear to be a major determinant of doxorubicin induced mortality in FU SY 1 SS or NHDF cultures , but may impact SW 982 cells via the overexpression of BAX relative to Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of hypoxic preconditioning on neural cell apoptosis and expression of Bcl 2 and Bax in cerebral ischemia reperfusion in rats . ^^^ In order to investigate the protective effect of hypoxic preconditioning on the cerebral ischemia reperfusion injury , the expression of Bcl 2 and Bax was detected by using immunohistochemical staining after 3 h cerebral ischemia followed by 1 , 6 , 12 , 24 and 48 h reperfusion respectively in rats treated with or without hypoxic preconditioning before cerebral ischemia . ^^^ Compared with control group ( cerebral ischemia reperfusion without hypoxic preconditioning ) , the expression of Bcl 2 was significantly increased , but that of Bax decreased in the hypoxic preconditioning group ( cerebral ischemia reperfusion with hypoxic preconditioning ) , both P < 0 . 05 . ^^^ It was suggested that hypoxic preconditioning may have protective effects on the cerebral ischemia reperfusion injury by inhibiting the apoptosis of neural cells , increase the expression of Bcl 2 and decrease the expression of Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Data analysis confirmed that atRA treatment induced apoptosis like cell death , as shown by decreased cell viability and increased fragmented DNA and sub G 1 fraction . atRA induced apoptosis was associated with upregulation of bcl 2 , translocation of bax protein to the mitochondria from the cytosol , activation of caspase 3 and cytochrome c release into cytosol . atRA induced apoptosis was abrogated by z DEVD fmk , a caspase 3 specific inhibitor , and z VAD fmk , a general caspase inhibitor , suggesting that the atRA induced cell death of MEPM cells occurs through the cytochrome c and caspase 3 dependent pathways . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TNF alpha levels were measured by ELISA kit ; VDR , Bcl 2 and Bax protein levels with Western blot analysis . ^^^ In UC patients an increase in Bcl 2 and Bax levels was observed incubating , PBMC with KH 1060 or anti TNF alpha or their combination . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both cytochrome c release and caspase activation triggered by p 38 MAPK activation and Bcl 2 phosphorylation are absent in embryonic fibroblasts from p38alpha knock out mice ( p38alpha ( / ) MEF ) , whereas they occur within 12 h of serum withdrawal in p38alpha ( + / + ) MEF ; moreover , they can be prevented by p 38 MAPK inhibitors and are not associated with the synthesis of the proapoptotic proteins Bax and Fas . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The purpose of the present study was to analyse the effects of naltrexone on the expression levels of proteins regulating the extrinsic ( FasL and Fas ) and the mitochondrial ( Bcl 2 , Bcl xL , Bad and Bax ) apoptotic pathways , as well as the active fragment of the executioner caspase 3 in the mouse brain . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistological evidences of Ginkgo biloba extract altering Bax to Bcl 2 expression ratio in the hippocampus and motor cortex of senescence accelerated mice . ^^^ Here , we studied the effect of G . biloba extract in the expression of Bax / Bcl 2 ratio , an important apoptotic index , in the hippocampus and motor cortex of the aging brain . ^^^ Bax and Bcl 2 expressions were examined with immunohistochemical methods . ^^^ In the hippocampus of G . biloba fed 9 month old mice , the ratio of Bax positive cell to Bcl 2 positive cell ( Bax / Bcl 2 expression ratio ) was 11 . 43 + / 3 . 11 ( mean + / SD ) ; significantly lower ( P < 0 . 05 ) than the Bax / Bcl 2 expression ratio of 20 . 99 + / 5 . 34 in the sucrose fed mice . ^^^ The Bax / Bcl 2 expression cell ratios , however , in the motor cortex were not significantly different between the two groups ( 2 . 22 + / 1 . 35 versus 2 . 27 + / 2 . 02 for the G . biloba and the sucrose fed mice , respectively ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of hyperbaric oxygen on cytochrome C , Bcl 2 and Bax expression after experimental traumatic brain injury in rats . ^^^ OBJECTIVE : To explore the effects of hyperbaric oxygen ( HBO ) treatment on the neuronal apoptosis at an earlier stage and the expressions of Cytochrome C ( Cyt C ) , Bcl 2 ( B cell lymphoma 2 family ) and Bax ( Bcl 2 associated 10 protein ) in rat brain tissues after traumatic brain injury ( TBI ) . ^^^ RESULTS : HBO treatment could up regulate the expression of Bcl 2 within 72 hours , reduce the release of Cyt C from mitochondria , attenuate the formation of dimeric Bax and alleviate the mitochondrial edema within 24 hours after TBI . ^^^ CONCLUSIONS : HBO treatment can alleviate neuronal apoptosis after TBI by reducing the release of Cyt C and the dimers of Bax and up regulating the expression of Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Proapoptotic multidomain Bcl 2 / Bax family proteins : mechanisms , physiological roles , and therapeutic opportunities . ^^^ In mammals , the multidomain proteins ( MDPs ) of the Bcl 2 family include Bax , Bak , and Bok . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Apoptosis was determined using TUNEL ( terminal deoxynucleotidyl transferase biotin dUTP nick end labeling ) assay and p 53 , p 27 , Bax , caspase 3 , caspase 8 , caspase 9 , Fas L , Bcl 2 , GATA 4 , Ki 67 , proliferating cell nuclear antigen ( PCNA ) , estrogen receptor , P receptor , and androgen receptor expression by immunohistochemistry . ^^^ DNA fragmentation and expression of Bax , caspase 3 , caspase 8 , caspase 9 , Fas L , Bcl 2 , GATA 4 , Ki 67 , and PCNA were observed in the whole ovary in both groups . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Resveratrol treatment for LNCaP cells was also found to result in a significant ( a ) loss of mitochondrial membrane potential , ( b ) inhibition in the protein level of antiapoptotic Bcl 2 , and ( c ) increase in proapoptotic members of the Bcl 2 family , i . e . , Bax , Bak , Bid , and Bad . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Both mRNA and protein expression of B cell lymphoma 2 ( Bcl 2 ) and Bax were detected by RT PCR and western blotting . ^^^ In the cells in which the Stat 6 signaling pathway was blocked , the levels of mRNA and protein Bcl 2 were significantly decreased , whereas those of Bax were significantly increased ( P < 0 . 01 ) . ^^^ The subsequent disorder of Bcl 2 / Bax expression may play an important part in that process . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Immunohistochemical stains were carried out on tissue microarrays to evaluate the expression of proteins involved in the G ( 1 ) S transition and proteins that regulate apoptosis including Rb , E2F1 , cyclin D 1 , CDK 4 , CDK 6 , p 27 ( KIP 1 ) , p 21 ( WAF1 / CIP1 ) , p 53 , Mdm 2 , Bcl 2 , and Bax . ^^^ The positive phenotypes identified were as follows : Rb , 39 . 1 % ; E2F1 , 69 . 6 % ; cyclin D 1 , 30 . 4 % ; CDK 4 , 100 % ; CDK 6 , 30 . 4 % ; 39 . 1 % ; p 27 ( KIP 1 ) , 47 . 8 % ; p 21 ( WAF1 / CIP1 ) , 39 . 1 % ; p 53 , 43 . 5 % ; Mdm 2 , 17 . 4 % ; Bcl 2 , 91 . 3 % ; and Bax , 100 % . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We have discovered that DNA damage induced p 53 Bcl2 binding is associated with decreased Bcl 2 Bax interaction and increased apoptotic cell death in a mechanism regulated by Bcl 2 ' s flexible loop regulatory domain ( FLD ) , since purified p 53 protein can disrupt the Bcl2 / Bax complex by directly binding to a negative regulatory region of the FLD ( amino acids [ aa ] 32 to 68 ) . ^^^ Thus , Bcl 2 ' s FLD contains both positive and negative regulatory regions which functionally regulate Bcl 2 ' s antiapoptotic activity by affecting Bax or p 53 binding . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inactivation of Bax , a pro apoptotic gene of the Bcl 2 family , in heterozygous Lurcher mutants ( Grid2Lc / + ) rescues approximately 60 % of the granule cells , but does not rescue Purkinje or olivary neurons . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In conclusion , the pro apoptotic Bcl 2 family members ( Bid and Bax ) are the key mediators in relaying the cell death signaling initiated by Andro from caspase 8 to mitochondria and then to downstream effector caspases , and eventually leading to apoptotic cell death . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The inhibition of apoptosis by hCG and dbcAMP was via the intrinsic pathway because the cytochalasin D treated cells stained intensely for Bax , whereas the cells treated with hormones , hCG , or dbcAMP stained predominantly for Bcl 2 . ^^^ Furthermore , this inhibition of apoptosis may be mediated in part by IGFBP 1 and prolactin and the alteration in the expression of Bcl 2 and Bax . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Twenty four hours after the treatment , the gene expressions of three important molecules related to apoptosis ( caspase 3 , Bax , and Bcl 2 ) in three experimental groups were evaluated by real time polymerase chain reaction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
At the same time , the expression level of pro apoptotic members ( Bax , Bad ) of the Bcl 2 family was increased under the chosen experimental conditions . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Cytochrome c was examined by immunohistochemical stain , and Fas , Fas ligand , Bax , Bcl 2 , and ERK activation ( p ERK / ERK ) were analyzed by Western blotting analysis . ^^^ In GRGD treated cells , cytochrome c was found released into cytoplasm by immunohistochemical study and the Bax expression was upregulated , whereas Bcl 2 expression was not changed . ^^^ The results indicated that alpha3beta1integrin is necessary for the prevention of the apoptosis of cultured rat podocytes , and that the signaling involves the Bax , Bcl 2 , and cytochrome c pathways . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of endothelial nitric oxide synthase ( eNOS ) , inducible nitric oxide synthase ( iNOS ) , Bax , and Bcl 2 were determined using Western blot . ^^^ Our results indicated that Hcy significantly reduced NO production , NOS activity , enhanced ET 1 / NO ratio and apoptosis , upregulated iNOS , Bax , and downregulated eNOS , Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Investigation into the mechanisms by which WT 1 regulates apoptosis has revealed that several bcl 2 family members are either direct or indirect WT 1 target genes , including bcl 2 itself , the pro apoptotic family members Bak and Bax , and the anti apoptotic family member Bfl 1 / A1 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Western blotting was used to detect changes in expression of Bax and Bcl 2 proteins resulting in increased Bax : Bcl 2 ratio that indicated a commitment of glioblastoma cells to apoptosis . ^^^ With the increased Bax : Bcl 2 ratio , cytochrome c was released from mitochondria to cytosol for sequential activation of caspase 9 and caspase 3 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was measured quantitatively by the terminal transferase UTP nick end labeling ( TUNEL ) method and confirmed by electron microscopic examination , the expression of Bax and Bcl 2 was immunohistochemically evaluated and quantified by Western blot analysis . ^^^ Greater Bcl 2 and attenuated Bax expression was found in the TMP treating rabbits . ^^^ CONCLUSION : These findings suggest that TMP has protective effects against spinal cord I / R injury by reducing apoptosis through regulating Bcl 2 and Bax expression . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Three groups of the Bcl 2 family proteins can be distinguished : the antiapoptotic proteins , like Bcl 2 and Bcl 10 L , the pro apoptotic members e . g . , Bax , Bak and the BH 3 only proteins . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
It was applied here to examine sequential changes in the expression of Bax and Bcl 2 proteins and in apoptotic cells to assess the relationship between penumbra and apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our previous studies have shown that depending on the neuronal cell type , glutamate induced apoptotic cell death was associated with regulation of genes such as Bcl 2 , Bax , and / or caspase 3 and mitochondrial cytochrome c . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hypoxia induced Bax and Bcl 2 protein expression , caspase 9 activation , DNA fragmentation , and lipid peroxidation in mitochondria of the cerebral cortex of newborn piglets : the role of nitric oxide . ^^^ The present study tests the hypothesis that cerebral hypoxia results in increased ratio of Bax / Bcl 2 , activation of caspase 9 , lipid peroxidation , and DNA fragmentation in mitochondria of the cerebral cortex of newborn piglets and that the inhibition of nitric oxide synthase by N nitro L arginine during hypoxia will prevent the events leading to mitochondrial DNA fragmentation . ^^^ Mitochondrial Bax and Bcl 2 proteins were determined by Western blot . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In general , SHR skeletal muscle had increased Bax protein , procaspase 3 protein , caspase 3 activity , cleaved poly ( ADP ribose ) polymerase protein , and DNA fragmentation as well as decreased Bcl 2 protein and a lower Bcl 2 to Bax ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results indicate that 4ICD is functionally similar to BH 3 only proteins , proapoptotic members of the BCL 2 family required for initiation of mitochondrial dysfunction through activation of the proapoptotic multi BH domain proteins BAX / BAK . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Effect of nitric oxide synthase inhibition during post hypoxic reoxygenation on Bax and Bcl 2 protein expression and DNA fragmentation in neuronal nuclei of newborn piglets . ^^^ Bax and Bcl 2 were analyzed by Western blot and DNA fragmentation was determined by agarose gel electrophoresis . ^^^ The results show that post hypoxic reoxygenation results in increased expression of Bax protein without affecting Bcl 2 protein and increased fragmentation of nuclear DNA , which are prevented by 7 NINA . ^^^ We propose that in addition to NO mediated nuclear DNA damage , the hypoxia induced increased ratio of Bax / Bcl 2 protein will lead to caspase activated cascade of hypoxic neuronal death during post hypoxic reoxygenation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Measures were mucosal apoptosis , proliferation , villous morphology , and apoptotic and proliferative mediators , such as caspase 3 and caspase 7 , Fas and Fas ligand , Bcl 2 , and Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Molecular changes explored through Western blot staining showed Tubeimoside 5 ( 1 ) decreased the expression levels of Bcl 2 protein and increased the expression levels of Bax protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Long term adrenalectomy did not change hippocampal pro apoptotic Bax or antiapoptotic Bcl 2 mRNA levels or protein content with respect to control . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The increased sensitivity of the cell lines to ionizing radiation was correlated with increased p 53 activity as well as with simultaneous up and downregulation of Bax and Bcl 2 , respectively . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
When rat brain mitochondrial voltage dependent anion channel ( VDAC ) , an outer mitochondrial membrane protein , interacts with Bcl 2 family proteins Bax and tBid , its pore size increases , leading to the release of cytochrome c and other apoptogenic molecules into the cytosol and causing cell death . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Moreover , changes in the transcription rates of p 53 , bcl 2 , and bax genes , on the one hand , and DNA fragmentation , on the other hand , were reported in PM exposed proliferating L 132 cells , revealing the occurrence of apoptotic events . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The antiapoptotic Bcl 2 and pro apoptotic Bax protein expressions were measured by immunocytochemical analysis . ^^^ Increases in apoptosis , Bax , lipid peroxidation product malondialdehyde , LDH , and decreases in Bcl 2 , superoxide dismutase , and glutathione peroxidase were observed in TNF alpha treated cells . ^^^ H2O2 10 microM did not cause significant lipid peroxidation ( 0 . 75 + / 0 . 03 nmol / mg of malondialdehyde protein ) as compared with control ( 0 . 70 + / 0 . 04 nmol / mg of malondialdehyde protein ) ( P > 0 . 05 ) but further enhanced TNF alpha induced lipid peroxidation , upregulated Bax , and down regulated Bcl 2 expression and enhanced TNF alpha induced cell apoptosis ( P < 0 . 05 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
A significantly increased activation of caspase 3 and 7 , decreased levels of antiapoptotic proteins Bcl 2 and Bcl 10 ( L ) , and increased levels of proapoptotic protein Bax was observed in the developing cerebral cortex of hypothyroid rats , compared with the euthyroid ( P < 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proapoptotic proteins Bak ( Bcl 2 antagonistic killer ) and Bax ( Bcl 2 associated 10 protein ) were depleted in livers from TCPOBOP treated CAR+ / + mice . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Inhibitory rate of xenograft tumor growth was estimated by measuring tumor volume ; expression of proliferating cell nuclear antigen ( PCNA ) , Bax and Bcl 2 proteins of xenograft tumor was detected using immunohistochemical S P method . ^^^ RESULTS : Tumor growth inhibitory rate , the positive expression rate of PCNA , Bax and Bcl 2 were 49 . 3 % , 58 . 2 % , 65 . 2 % and 59 . 2 % in rhGH+L OHP group respectively ; 46 . 6 % , 62 . 5 % , 59 . 7 % and 64 . 7 % in L OHP group ; 5 . 0 % , 82 . 7 % , 23 . 2 % and 82 . 2 % in rhGH group and 0 , 77 . 8 % , 23 . 5 % and 80 . 3 % in control group . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
GPs treatment significantly blocked glutamate induced decrease in levels of Bcl 2 and increase in Bax , leading to a decrease in glutamate induced apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , LIG provided a great increase in Bcl 2 expression as well as a significant decrease in Bax and caspase 3 immunoreactivities in the ischemic cortex . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Radiation therapy induced changes in apoptosis and its major regulatory proteins , Bcl 2 , Bcl XL , and Bax , in locally advanced invasive squamous cell carcinoma of the cervix . ^^^ The major apoptotic regulatory molecules include Bcl 2 , Bcl XL ( antiapoptotic ) , and Bax ( proapoptotic ) proteins . ^^^ The TUNEL assay was performed to detect apoptotic nuclei and Bcl 2 , Bcl XL , and Bax proteins detected by immunohistochemistry ( IHC ) . ^^^ The Bcl 2 and Bax IHC scores and the Bcl 2 / Bax ratio did not correlate with AI in the 2 groups . ^^^ CONCLUSIONS : RT for invasive squamous cell carcinoma of cervix results in increased apoptotic cell death with the up regulation of Bax , a proapoptotic protein , and the down regulation of Bcl XL , an antiapoptotic protein , without any significant change in the levels of Bcl 2 . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Our results suggest that the protective effects of adiponectin on acetaldehyde induced apoptosis may be ascribed to ability to induce the expression of anti oxidant enzymes and to regulate Bcl 2 and Bax expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In parallel , p 38 MAP kinase activity down modulation , along with low caspase 3 activity , and no modification of Bax and Bcl 2 levels , supported such evidence . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The apoptosis was determined by microscopic examination of apoptotic morphology , determination of DNA fragmentation by electrophoresis , activation of caspase 3 , and protein expression of procaspase 3 , poly ( ADP ribose ) polymerase ( PARP ) cleavage , Bcl 2 , and Bax . ^^^ Concomitantly , ST treatments led to an increase in the proapoptotic Bax levels , while Bcl 2 expression was decreased . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction and immunoblotting results indicated that treatments of cells with beta lapachone resulted in down regulation of anti apoptotic Bcl 2 and Bcl 10 ( L ) and up regulation of pro apoptotic Bax expression . beta Lapachone induced apoptosis was associated with a proteolytic activation of caspase 3 and 9 and degradation of poly ( ADP ribose ) polymerase protein . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , LPS ( 1 microgml ( 1 ) ) induced decrease in Bcl 2 and increase in Bax protein expression were fully reversed by cilostazol ( 10 microM ) and dibutyryl cAMP ( 100 microM ) , all of which were antagonized by Rp cAMPs ( 200 microM ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
BACKGROUND : The aim of this study was to examine the expressions of the bcl 2 , bax , fas and c myc apoptosis related genes in benign prostatic hyperplasia ( BPH ) and prostate carcinoma ( CaP ) to determine whether significant differences exist within each disease and between the two groups of patients . ^^^ PATIENTS AND METHODS : Tissue specimens from 51 cases of BPH and 27 cases of CaP were examined for bcl 2 , bax , fas and c myc expression by reverse transcriptase PCR ( RT PCR ) . ^^^ RESULTS : In BPH , bcl 2 and bax gave the weakest signals ( p < 0 . 001 ) . ^^^ Both bcl 2 and bax were expressed at higher levels in CaP than in BPH ( p < 0 . 02 ) . ^^^ The bcl 2 / bax ratio was lower in CaP than in BPH ( p < 0 . 001 ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Down regulation of bcl 2 and up regulation of bax mRNA by beta eleostearic acid were also greater than by alpha eleostearic acid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
RESULTS : At a 5 10 microM dose level , ( ) gossypol significantly enhanced apoptosis measured by DNA fragmentation . ( ) Gossypol caused apoptosis in DU 145 cells through the down regulation of Bcl 2 and Bcl xL and the up regulation of Bax at the mRNA and protein levels . ( ) Gossypol also activated caspases 3 , 8 and 9 and increased PARP [ poly ( ADP ribose ) polymerase ] cleavage . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The drug effects on the expression of the beta tubulin isotypes , Bcl 2 , Bax , Bcl XL and proteomic profiles were evaluated by immunobloting and SELDI mass spectrometry in tumor xenografts dosed at 0 . 5 MTDs . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The mRNA and protein levels of c Myc , Bcl 10 ( L ) and Bcl 2 were downregulated , whereas the expression of p 53 and bax was upregulated in mPER 2 overexpressing LLC cells compared with control cells transferred with empty plasmid . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bax and bcl 2 mRNA expression were determined by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Furthermore , ioversol induced a decrease in the expression for bcl 2 mRNA and an increase for bax mRNA . ^^^ In addition , irbesartan reversed the ioversol induced increase in bax mRNA and decrease in bcl 2 mRNA . ^^^ CONCLUSION : Ioversol induced NRK 52E cells apoptosis in a concentration and time dependant manner via an increase in oxidative stress and subsequent to the increase in mRNA expression for bax and reduction in bcl 2 mRNA . ^^^ Irbesartan attenuated the ioversol induced apoptosis in NRK 52E cells by reducing oxidative stress and reversing the enhancement of bax mRNA and the reduction in bcl 2 mRNA . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
To test this hypothesis , we treated isolated mitochondria with Bcl 2 antagonistic peptides ( derived from the BH 3 domain of Bax or Bid ) . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Hint 1 is involved in the regulation of apoptotic pathways by inducing an up regulation of p 53 expression coinciding with an up regulation of the proapoptotic factor Bax and a concomitant down regulation of the apoptosis inhibitor Bcl 2 . ^^^ Knockdown of Hint 1 by short hairpin RNA resulted in down regulation of p 53 and Bax but had no effect on Bcl 2 expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis as measured by formation of apoptotic bodies , flow cytometric analysis , activation of caspase 3 and 8 , and expressions of apoptosis related genes such as bcl 2 and bax showed high degree of correlation with comet tail moment . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The expression levels of Bcl 2 family member mRNA and protein , including Bcl 2 , Bcl xL and Bax , were also investigated . ^^^ Our results indicated that : ( 1 ) the hippocampus volume significantly increased from P 5 to P 60 , although the number of neurons remained stable in all studied stages ; ( 2 ) the number of apoptotic cells was highest at P 45 , based either on the Nissl staining or on the immunohistochemistry for caspase 3 ; ( 3 ) Bcl 2 mRNA expression was high from P 5 to adulthood , while Bax mRNA declined abruptly from P 5 to adulthood , and Bcl 10 mRNA was high after P 45 . ^^^ Bcl 2 protein was only detected at P 5 and P 15 , while detection of Bcl xL and Bax proteins paralleled levels of mRNA expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expressions of cyclin D 1 , p 53 , bcl 2 , and bax in infiltrative ductal carcinoma of the breast : correlations with clinicopathologic characteristics . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
COX 2 inhibition led to a rapid down regulation of myeloid cell leukemia 1 ( Mcl 1 ) , an antiapoptotic member of the Bcl 2 family , followed by translocation of Bax to mitochondria and cytochrome c release from mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Anti beta 2 glycoprotein 1 antibodies affect Bcl 2 and Bax trophoblast expression without evidence of apoptosis . ^^^ In all the cultures we evaluated : ( 1 ) Bcl 2 and Bax mRNA and protein expression by Western blot and reverse transcription polymerase chain reaction ( RT PCR ) , respectively ; ( 2 ) DNA fragmentation by a commercial ELISA kit and by agarose gel electrophoresis ; and ( 3 ) the percentage of cells reactive with the monoclonal antibody ( MAb ) M 30 by indirect immunofluorescence . ^^^ The results were : Bcl 2 / Bax ratio increased in untreated trophoblast cells during the time of culture , showing the highest values detectable after 72 h ( 2 . 68 and 2 . 28 at protein and mRNA levels , respectively ) . ^^^ Cell incubation with anti beta2GPI MAbs induced a significant Bcl 2 / Bax ratio reduction in comparison with untreated cells ( 1 . 22 and 1 . 28 at protein and mRNA levels , respectively , after 72 h incubation ) . ^^^ In Conclusion , anti beta2GPI antibodies react with trophoblast cells and reduce the Bcl 2 / Bax ratio , but without any clear apoptotic effect . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
However , the expressions of p 53 or pro or antiapoptotic Bcl 2 family proteins ( Bax , Bcl 2 , Bcl 10 ( L ) and Mcl 1 ) were not affected in any of the cell lines even after prolonged exposure to FB ( 1 ) at high doses . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , apoptosis induced with julibroside J 8 was associated with an increase in expression of the apoptosis inducer Bax , and a significant reduction in expression of the apoptosis suppressor Bcl 2 in mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The present study aimed to evaluate the underlying mechanisms of apoptosis through modulation of Bax , Bcl 2 and cytochrome c protein expressions in a human pro myelocytic leukemia ( HL 60 ) cell line , as well as the potential of the CV extract as anti leukemia agent using the athymic mouse xenograft model . ^^^ Meanwhile , expression of anti apoptotic protein Bcl 2 was concomitantly down regulated , as reflected by the increased Bax / Bcl 2 ratio . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Starting at 6 h , the apoptotic index increased in a time dependent manner , in correlation with the expression of HIF 1alpha , Bcl 2 , Bcl xL , Bax and cleaved Caspase 9 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis was evaluated with deoxyribonucleotidyl transferase mediated dUTP biotin nick end labeling assay , and BAX and BCL 2 expressions were used to detect induction of and protection from proapoptotic mechanisms , respectively . ^^^ Subjects with structural heart damage of alcoholic or hypertensive origin showed higher apoptotic indexes in deoxyribonucleotidyl transferase mediated dUTP biotin nick end labeling , BAX , and BCL 2 assays as compared with control subjects ( P < . 001 for all ) . ^^^ Moreover , New York Heart Association class 1 alcoholic patients displayed higher BAX and BCL 2 expressions as compared with control subjects . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Its three factions of interacting proteins include the BH 3 only proteins ( e . g . , Bim , Puma , Bad , Noxa ) , which transduce diverse cytotoxic signals to the mammalian pro survival proteins ( Bcl 2 , Bcl 10 ( L ) , Bcl w , Mcl 1 , A 1 ) , whereas Bax and Bak , when freed from pro survival constraint , provoke the mitochondrial permeabilization that triggers apoptosis . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Apoptosis and the activity of ceramide , Bax and Bcl 2 in the lungs of neonatal rats exposed to limited and prolonged hyperoxia . ^^^ This is done by examining the morphologic changes of apoptosis , the expression of ceramide , an important mediator of apoptosis , the expression of inflammatory mediators represented by IL 1beta and the expression of 2 proto oncogenes that appear to modulate apoptosis ( Bax and Bcl 2 ) . ^^^ Sections were also incubated with anti Bcl 2 and anti Bax antisera . ^^^ Bcl 2 and Bax were quantitated by immunohistochemistry . ^^^ The early rise in Bax and ceramide appears to overcome the anti apoptotic activity of Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The results here demonstrate evidence for approximately 25 % apoptosis after treatment with calcium salicylate , which up regulatd the expression of p 53 , p 21 and Bax , and down regulated Bcl 2 in HT 1080 cells . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Melatonin protects against hydrogen peroxide induced cell death signaling in SH SY5Y cultured cells : involvement of nuclear factor kappa B , Bax and Bcl 2 . ^^^ In addition , induction of Bcl 2 and Bax proteins was demonstrated in SH SY5Y cultured cells treated with H ( 2 ) O ( 2 ) , whereas the induction of Bax but not Bcl 2 was diminished by melatonin . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Amygdalin induces apoptosis through regulation of Bax and Bcl 2 expressions in human DU 145 and LNCaP prostate cancer cells . ^^^ Treatment with amygdalin increased expression of Bax , a pro apoptotic protein , decreased expression of Bcl 2 , an anti apoptotic protein , and increased caspase 3 enzyme activity in DU 145 and LNCaP prostate cancer cells . ^^^ Here , we have shown that amygdalin induces apoptotic cell death in human DU 145 and LNCaP prostate cancer cells by caspase 3 activation through down regulation of Bcl 2 and up regulation of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The protein expression ratio of Bcl xL / Bax and Bcl 2 / Bax was down regulated and uncarinic acid E induced apoptosis involves the initial phase mediated by the balance among Bcl xL , Bcl 2 and Bax proteins , resulting in cytochrome c release from the mitochondria . ^^^ Taken together , uncarinic acid E induces apoptosis in HepG 2 cells via accumulation of p 53 , alters the Bax / Bcl 2 ratio , and activates caspases , resulting in cytochrome c release from the mitochondria . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Furthermore , there is little information about expressions and regulations of Bax , Bcl 2 , and p 53 in lead induced apoptosis , which are critical regulators of mitochondrial stability . ^^^ The present study was undertaken to determine whether lead could induce DNA damage and apoptosis in PC 12 cells , and the involvement of Bax , Bcl 2 , p 53 , and caspase 3 in this process . ^^^ The results showed that lead could induce DNA damage and apoptosis in PC 12 cells , accompanying with upregulation of Bax and downregulation of Bcl 2 . ^^^ Therefore , it suggests that lead can induce activation of p 53 by DNA damage , which may lead to imbalance of Bax / Bcl 2 and mitochondrial dysfunction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In addition , RT PCR was used to analyze the levels of Bcl 2 and Bax mRNA . ^^^ Bcl 2 , Bax and caspase 3 protein expressions were determined by Western blot analysis . ^^^ The expression of Bcl 2 was down regulated , while the expression of Bax up regulated . ^^^ These results suggest that OP may induce Sertoli cell apoptosis by regulation of Bcl 2 / Bax and caspase 3 activation . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We show that GT induces apoptotic cell death by activating the proapoptotic Bcl 2 family member Bak , but not Bax , to elicit the generation of reactive oxygen species , the mitochondrial release of apoptogenic factors , and caspase 3 activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and bax expression and prostate cancer outcome in men treated with radiotherapy in Radiation Therapy Oncology Group protocol 86 10 . ^^^ PURPOSE : Bcl 2 and bax are proteins with opposing roles in apoptosis regulation ; yet abnormal expression of either has been associated with failure after radiotherapy ( RT ) . ^^^ In this study we examined bcl 2 and bax expression as predictive markers in men treated with radiotherapy + / androgen deprivation on Radiation Therapy Oncology Group ( RTOG ) protocol 86 10 . ^^^ EXPERIMENTAL DESIGN : Suitable archival diagnostic tissue was obtained from 119 ( 26 % ) patients for bcl 2 analysis and 104 ( 23 % ) patients for bax analysis . ^^^ Cox proportional hazards multivariate analysis was used to determine the relationship of abnormal bcl 2 and bax expression to the end points of local failure , distant metastasis , cause specific mortality , and overall mortality . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
In contrast , the expressions of Bcl 2 and Bax , regulators for intrinsic apoptotic signaling through the mitochondria , did not change . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
EGF increased the expression of the Bcl 10 ( L ) protein , an antiapoptotic member of the Bcl 2 family , but not that of other anti ( Bcl 2 ) or proapoptotic ( Bad and Bax ) protein members . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Expression of Bcl 2 and Bax proteins were determined with flow cytometry using respective monoclonal antibodies . ^^^ MSG treatment induced down regulation of Bcl 2 protein while Bax protein levels were not significantly changed . ^^^ The temporal profile of Bcl 2 and Bax expression , after MSG treatment , suggest that down regulation of Bcl 2 protein and resulting change of Bcl 2 / Bax protein ratio may be an important event in thymocyte apoptosis , triggered by MSG ( Tab . 1 , Fig . 3 , Ref . 36 ) . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
The proapoptotic activity of NO involves the rise in the tumor suppressor p 53 and the accumulation and targeting of proapoptotic members of the Bcl 2 family , in particular Bax and the release of cytochrome c from the mitochondria . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
DHA promoted the early expression of Bcl 2 , decreased Bax expression and reduced caspase 3 activation in photoreceptors . ^^^ This leads to the regulation of Bcl 2 and Bax expression , thus preserving mitochondrial membrane potential and inhibiting caspase activation . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
TUDCA reduced nuclear fragmentation as well as caspase 2 and 6 , but not caspase 8 activities . p 53 activity , and Bcl 2 and Bax changes , were also modulated by TUDCA . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
B . henselae mediated inhibition of apoptosis , as indicated by gene expression of Bax and Bcl 2 , was also shown to be IL 8 dependent in endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Using immunoperoxidase on paraffin sections , bcl 2 and bax immunoreactivities have been evidenced , and CD 34 positive microvessels counted ; ultra thin sections have also been observed . ^^^ After six months of iodine overload , apoptotic thyrocytes were ten times more numerous ; CD 34 positive endothelial cells were diminished by one half bcl 2 immunoreactivity disappeared in thyrocytes and a bax one appeared in thyroid follicular and endothelial cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Further , leukemia cell lines over expressing sorcin also showed up regulation of Bcl 2 , along with decreased level of Bax . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Gossypol induces Bax / Bak independent activation of apoptosis and cytochrome c release via a conformational change in Bcl 2 . ^^^ We found that gossypol , but not other Bcl 2 interacting molecules , induced cyto c release and loss of mitochondrial membrane potential ( delta psi m ) independently of mPTP and Bak / Bax activation . ^^^ Furthermore , we found that gossypol induced an allosteric change in Bcl 2 in both bak ( / ) / bax ( / ) cells and Bcl 2 overexpressing cells . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Ladostigil dose dependently decreased cell death via inhibition of the cleavage and prevention of caspase 3 activation ( IC50=1 . 05 microM ) through a mechanism related to regulation of the Bcl 2 family proteins , which resulted in reduced levels of Bad and Bax and induced levels of Bcl 2 gene and protein expression . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Besides , CCM caused an increase in Bax : Bcl 2 ratio , and mitochondrial release of cytochrome c , Second mitochondrial activator of caspases / Direct IAP binding protein with low pI ( Smac / Diablo ) , and apoptosis inducing factor ( AIF ) indicating involvement of mitochondria mediated pathway as well . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
We determined immunohistochemically the expression of proliferation markers PCNA and Ki 67 , Bax , p 53 , Bcl 2 , p21waf1 , p27kiP1 and compared them with the expression of the androgen receptor ( AR ) . ^^^ The expression of PCNA correlated strongly with p 27 kip1 and Bax , while the expression of Ki 67 correlated most strongly with p 27 kip1 and Bcl 2 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
These apoptotic markers were suppressed by the addition of ( 20S ) Rg 3 . ( 20S ) Rg 3 increased the expression of Bax and conversely decreased Bcl 2 . ( 20S ) Rg 3 potently induced a rapid and sustained Akt activation and Bad phosphorylation , resulting in the inhibition of mitochondrial cytochrome c release . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Influence of prostaglandin A 2 on Bax , Bcl 2 and PCNA expression in MCF 7 cells . ^^^ The effects of 20 microg / mL exogenous prostaglandin A ( 2 ) ( PGA ( 2 ) ) were determined on Bax , Bcl 2 and proliferating cell nuclear antigen ( PCNA ) expression levels in MCF 7 cells . ^^^ The Bax / Bcl 2 ratio for PGA ( 2 ) exposed cells was 2 . 7 . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bcl 2 and proliferating cell nuclear antigen expression was downregulated , whereas Fas and Bax were upregulated in TPN livers . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Two members of the Bcl 2 family , Bax and Bak , change intracellular location early in the promotion of apoptosis to concentrate in focal clusters at sites of mitochondrial division . ^^^ Our results show that Bax and Bak regulate mitochondrial dynamics in healthy cells and indicate that Bcl 2 family members may also regulate apoptosis through organelle morphogenesis machineries . . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
Bak and Bax are in the Bcl 2 family and counteract the antiapoptotic function of Bcl 2 . ^^^ We hypothesized that ( a ) survival benefit in HRPC patients treated with taxanes is determined by the presence of Bcl 2 protein and ( b ) altered expression of Bak and Bax protein caused by genetic mutation is associated with biological aggressiveness of prostate cancer . ^^^ Surgical specimens of localized prostate cancer and biopsy specimens of HRPC were used for immunostaining of Bcl 2 , Bak , and Bax as well as DNA extraction . ^^^
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q07812 and P10415	Pubmed	SVM Score :0.0
NA