| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| Mitotic , tyrosine phosphorylated Sam 68 bound selectively to recombinant SH 2 domains with significantly different affinities ( c Src approximately Ras GTPase activating protein > p 85 alpha ( amino terminal ) > Grb 2 > > p 85 alpha ( COOH terminal ) ) . ^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| Formation of this intramolecular SH 3 ligand complex prevents the Itk SH 3 domain and proline rich region from interacting with their respective protein ligands , Sam 68 and Grb 2 . ^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| The mammalian members include Sam 68 , which is a target of Src , Fyn , and Grb 2 , and the newly cloned mouse quaking proteins ( qkI ) necessary in early embryogenesis and myelination . ^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| During mitosis , Sam 68 undergoes tyrosine phosphorylation , which negatively regulates its nucleic acid binding properties and mediates the interaction of Sam 68 with critical SH 2 containing signaling proteins such as Grb 2 , PLC gamma 1 and Ras GAP . ^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domains of c Src , phosphatidylinositol 3 OH kinase , phospholipase C gamma and Grb 2 protein ( containing two SH 3 domains ) , but not other SH 3 domains tested , were capable of binding Sam 68 in vitro . ^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.78166427 |
| A direct interaction between Sam 68 and the two src kinases involved in T cell activation , p 59 ( fyn ) and p 56 ( lck ) , as well as a partnership of Sam 68 with various key downstream signaling molecules , like phospholipase Cgamma 1 and Grb 2 , has been shown . 0.78166427^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.73700274 |
| This ability of UCS15A was not restricted to Src SH 3 mediated protein protein interactions , since the drug was capable of disrupting the in vivo interactions of Sam 68 with other SH 3 domain containing proteins such as Grb 2 and PLCgamma . 0.73700274^^^ |
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| Interacting proteins: Q07666 and P62993 |
Pubmed |
SVM Score :0.0 |
| Sam 68 associates with the SH 3 domains of Grb 2 recruiting GAP to the Grb 2 SOS complex in insulin receptor signaling . ^^^ Besides , we sought to further study the association of Sam 68 with the Ras GAP pathway by assessing the interactions with SH 3 domains of Grb 2 . ^^^ We employed GST fusion proteins containing the SH 3 domains of Grb 2 ( N or C ) , and recombinant Sam 68 for in vitro studies . ^^^ In vivo studies of protein protein interaction were assessed by co immunoprecipitation experiments with specific antibodies against Sam 68 , GAP , Grb 2 , SOS , and phosphotyrosine ; and by affinity precipitation with the fusion proteins ( SH 3 Grb2 ) . ^^^ Sam 68 is constitutively associated with the SH 3 domains of Grb 2 and it does not change upon insulin stimulation , but Sam 68 is Tyr phosphorylated and promotes the association of GAP with the Grb 2 SOS complex . ^^^ |
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