| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The KH domain of SF 1 is embedded in a 160 amino acid sequence that is shared with human Sam 68 , a target of Src during mitosis , as well as Caenorhabditis elegans GLD 1 and mouse Qkl , both of which play roles during cellular differentiation . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Only one peptide inhibited binding of Sam 68 to the p85alpha SH 3 domain , whereas several peptides inhibited binding of Sam 68 to c src SH 3 domain , suggesting that Sam 68 uses different proline rich motifs to bind to different SH 3 domains . ^^^ A peptide derived from residues 32 44 of Sam 68 which fits the class 2 SH 3 domain binding consensus sequence inhibited binding of Sam 68 to both p85alpha SH 3 domain and c src SH 3 domain , but with differential potency , suggesting a differential affinity of these SH 3 domains for this proline rich motif . . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| By examining the ability of different SH 3 domains to interact with deletion variants of Sam 68 and WASP , we demonstrated that the Itk SH 3 domain and the SH 3 domains of Src family kinases bind to overlapping but distinct sets of proline rich regions in Sam 68 and WASP . . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grap also associates via its SH 3 domains with Sos , the Ras guanine nucleotide exchange factor ; with dynamin , a GTPase involved in membrane protein trafficking ; and with Sam 68 , a nuclear RNA binding protein that serves as a substrate of Src kinases during mitosis . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The major target of c Src during mitosis is SAM 68 , a RNA binding protein ordinarily localized to the nucleus . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| These data suggest that Sam 68 participates in the signal transduction pathway downstream of TCR coupled Src family kinases Fyn and Lck in lymphocytes , that is not only in the mitotic pathway downstream of c Src in fibroblasts . . ^^^ Interaction between Sam 68 and Src family tyrosine kinases , Fyn and Lck , in T cell receptor signaling . ^^^ Purification and microsequencing of this protein revealed that it was the RNA binding protein , Sam 68 ( Src associated in mitosis , 68 kDa ) . ^^^ Sam 68 was associated with the Src homology 2 and 3 domains of Fyn and also those of another Src family kinase , Lck . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The mammalian members include Sam 68 , which is a target of Src , Fyn , and Grb 2 , and the newly cloned mouse quaking proteins ( qkI ) necessary in early embryogenesis and myelination . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 ( Src associated in mitosis ) is an SH 3 ( Src homology 3 ) , SH 2 ( Src homology 2 ) , and RNA binding protein which associates with and is tyrosine phosphorylated by wild type and activated forms of c Src in a mitosis specific manner . ^^^ Phosphorylation of the Src substrate Sam 68 by Cdc 2 during mitosis . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 , a specific target of the Src tyrosine kinase in mitosis , possesses features common to RNA binding proteins , including a K homology ( KH ) domain . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Employing antibodies specific to two p 85 isoforms , p85alpha and p85beta , we demonstrate that HTC IR cells express both p 85 isoforms , and these isoforms induce the formation of similar signaling complexes in response to insulin . p 60 70 , present in both alpha p85alpha and alpha p85beta immunoprecipitates , is a GAP associated protein , but is distinct from the p 68 src associated protein in mitosis ( Sam 68 ) by several criteria . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| It has been further established that Sam 68 is a substrate of src kinases in mitosis and that it is not associated with p120GAP in transformed fibroblasts . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Characterization of Sam 68 like mammalian proteins SLM 1 and SLM 2 : SLM 1 is a Src substrate during mitosis . ^^^ Sam 68 , the 68 kDa Src substrate associated during mitosis , is an RNA binding protein with signaling properties that contains a GSG ( GRP 33 , Sam 68 , GLD 1 ) domain . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Therefore these phenotypes do not depend upon the recruitment through the SH 3 domain of src of RNA binding proteins ( Sam 68 , hnRNP K ) . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| A close relationship between Sam 68 , a tyrosine and proline rich RNA binding protein , and Src protein tyrosine kinases ( PTK ) has already been established , also in T lymphocytes . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The interaction and colocalization of Sam 68 with the splicing associated factor YT 521 B in nuclear dots is regulated by the Src family kinase p 59 ( fyn ) . ^^^ Here , we describe a tyrosine phosphorylated nuclear protein , YT 521 B , and show that it interacts with the nuclear transcriptosomal component scaffold attachment factor B , and the 68 kDa Src substrate associated during mitosis , Sam 68 . ^^^ The Src family kinase p 59 ( fyn ) mediated tyrosine phosphorylation of Sam 68 negatively regulates its association with YT 521 B , and overexpression of p 59 ( fyn ) dissolves nuclear dots containing YT 521 B . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 , a nuclear RNA binding protein , is a major substrate of the Src tyrosine kinase in mitotic cells . ^^^ Salpalpha is tyrosine phosphorylated when expressed in Rous sarcoma virus infected chicken embryo fibroblasts ( RSV CEF ) ; unlike Sam 68 , however , Salpalpha does not co precipitate with 5 Src . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| In the course of screening a lambdagt 11 human leukemic T cell cDNA expression library with an antibody specific to the mitotic target of Src , Sam 68 , we identified and cloned a cDNA encoding a novel protein with a predicted molecular mass of 51 . 4 kDa . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that the proposed adapter protein for Src kinases , Sam 68 , is a ligand whose proline rich motifs interact with the SH 3 domains of p 59 ( fyn ) and phospholipase Cgamma 1 as well as with the WW domains of FBP 30 and FBP 21 . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| In contrast , SAM 68 and whole cell tyrosine phosphorylation by c SRC was unaffected , indicating that the SH 1 ( KD ) mutant did not inhibit c SRC tyrosine kinase activity in general . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Indeed , three amino acids important for ligand binding in Src SH 3 were replaced in the Slap SH 3 sequence ; Slap SH 3 did not bind to the Src SH 3 partners p85alpha , Shc , and Sam 68 in vitro , and the chimeric tyrosine kinase Slap / SrcK , composed of SlapDeltaC fused to the SH 2 linker kinase sequence of Src , was not regulated in vivo . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that Sik and BRK associate with the RNA binding protein Sam 68 ( Src associated during mitosis , 68 kDa ) . ^^^ While Sam 68 is a substrate for Src family kinases during mitosis , Sik / BRK is the first identified tyrosine kinase that can phosphorylate Sam 68 and regulate its activity within the nucleus , where it resides during most of the cell cycle . . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 is a multimeric 68 kDa RNA binding nuclear protein of unknown function that interacts with , and is tyrosine phosphorylated by , the oncogenic protein Src during mitosis . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Two of these phosphoproteins were identified as bona fide src substrates , cortactin and Sam 68 . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The 68 kDa Src substrate associated during mitosis ( Sam 68 ) is an RNA binding protein with Src homology ( SH ) 2 and 3 domain binding sites . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| In the present study we have examined the ability of UCS15A to disrupt the interaction of Src SH 3 with Sam 68 , both in vivo and in vitro . ^^^ This ability of UCS15A was not restricted to Src SH 3 mediated protein protein interactions , since the drug was capable of disrupting the in vivo interactions of Sam 68 with other SH 3 domain containing proteins such as Grb 2 and PLCgamma . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The 68 kDa Src substrate associated during mitosis ( Sam 68 ) is an RNA binding protein with Src homology ( SH ) 2 and 3 domain binding sites . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Using this approach we have confirmed the domain binding profile of the signalling molecule Sam 68 ( Src associated during mitosis 68 ) , and have identified a new binding profile for the core small nuclear ribonucleoprotein SmB ' . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 is an RNA binding protein that is tyrosine phosphorylated by Src during mitosis and has been postulated to have a role in cell cycle control by modulating RNA metabolism . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 is a target of the c Src tyrosine kinase . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| In T cells , Fyn appears to be the active Src kinase in rafts , but Sam 68 is not expressed in rafts , and its distinct phosphorylation by Fyn and Lck is not affected by raft dispersion . ^^^ Mainly , Lck phosphorylates Sam 68 when the interaction between the SH 3 domain and the SH 2 catalytic domain linker is altered in heterologous Src molecules or after mutating key residues in the linker that increase the accessibility of the SH 3 domain . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| We identified the Src substrate and RNA binding protein Sam 68 as novel CH 3 binding protein . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Brk has been shown to phosphorylate two proteins in vivo : Sam 68 . a substrate for Src in mitosis that can substitute for Rev in nuclear export of RNAs ; and BKS , a novel adaptor molecule . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src substrate Sam 68 showed an increased phosphorylation in mutants with high Src activity , suggesting that p 60 ( c Src ) triggers downstream signaling in these cells . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| We investigated whether phosphatidylinositol 3 kinase ( PI3K ) and 68 kDa Src associated during mitosis ( SAM 68 ) are involved in angiotensin 2 ( ANG 2 ) growth signaling in vascular smooth muscle cells ( VSMCs ) from spontaneously hypertensive rats ( SHR ) . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylation of a 68 kDa protein ( Sam 68 ) also increases at this time , and recent studies have shown that Src and Sam 68 interact . ^^^ The relationship between p 62 and Sam 68 , and their roles in Src signalling , need to be clarified , but these findings suggest that Src may participate in regulating RNA processing during the cell cycle . . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 ( Src associated in mitosis ; 68 kDa ) is a member of the STAR ( signal transduction and activation of RNA ) family of KH domain containing RNA binding proteins . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 , the substrate of Src in mitosis , belongs to the family of RNA binding proteins . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation and in vitro kinase assays reveal rapid GC induced down modulation of Lck and Fyn kinases using SAM 68 ( Src [ pp60c src ] associated in mitosis 68 kDa ) as a substrate . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Recently , it has been found that Sam 68 , the substrate of Src associated in mitosis , is a functional homologue of Rev , and a synergistic activator of Rev activity . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src substrate associated in mitosis of 68 kDa ( Sam 68 ) is a KH type RNA binding protein that has been shown to regulate several aspects of RNA metabolism ; however , its physiologic role has remained elusive . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 ( Src associated in mitosis ; 68 kDa ) is an RNA binding protein and substrate of Src family kinases . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Sam 68 plays an important role as a multiple functional RNA binding nuclear protein in cell cycle progress , RNA usage , signal transduction , and tyrosine phosphorylation by Src during mitosis . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.66159837 |
| Sam 68 ( Src associated in mitosis 68 kDa ) is a protein that associates with and is tyrosine phosphorylated by Src in a mitosis specific manner , thereby raising the possibility of a role for Src in the regulation of the cell cycle . 0.66159837^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.79469194 |
| Sam 68 , a nuclear RNA binding protein , binds to Src and is phosphorylated at tyrosine residues in an M phase specific manner . 0.79469194^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.77935097 |
| A direct interaction between Sam 68 and the two src kinases involved in T cell activation , p 59 ( fyn ) and p 56 ( lck ) , as well as a partnership of Sam 68 with various key downstream signaling molecules , like phospholipase Cgamma 1 and Grb 2 , has been shown . 0.77935097^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.53168912 |
| Interestingly , we found that Sam 68 , a RNA binding protein phosphorylated by Src in mitosis , is phosphorylated only in prostate tumors expressing tr Kit . 0.53168912^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Functional interaction between c Src and its mitotic target , Sam 68 . ^^^ We have examined the mechanism and functional consequence of the interaction of c Src with this protein , Sam 68 ( Src associated in mitosis , 68 kDa ) . ^^^ In whole cell homogenates , Sam 68 was the predominant substrate and binding partner of overexpressed c Src . ^^^ Mitotic , tyrosine phosphorylated Sam 68 bound selectively to recombinant SH 2 domains with significantly different affinities ( c Src approximately Ras GTPase activating protein > p 85 alpha ( amino terminal ) > Grb 2 > > p 85 alpha ( COOH terminal ) ) . ^^^ The results suggest that the selection of Sam 68 as a mitotic target by c Src is the result of highly specific interaction with SH 2 and SH 3 domains and that this interaction may modulate the RNA binding activity of Sam 68 . . ^^^ |
|
| Interacting proteins: Q07666 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sam 68 is a 68 kDa protein that associates with and is phosphorylated by the c Src kinase at mitosis . ^^^ The SH 3 domains of c Src , phosphatidylinositol 3 OH kinase , phospholipase C gamma and Grb 2 protein ( containing two SH 3 domains ) , but not other SH 3 domains tested , were capable of binding Sam 68 in vitro . ^^^ Evidence for SH 3 domain directed binding and phosphorylation of Sam 68 by Src . ^^^ Mutation of Sam 68 SH3 binding sites further indicated that the SRC SH 3 domain mediates binding of Src to unphosphorylated Sam 68 . ^^^ Phosphorylation of Sam 68 by Src kinase was inhibited when the Src SH 3 binding site of Sam 68 was mutated or when corresponding peptides were added to in vitro kinase reactions indicating that binding of the Src SH 3 domain to a specific site near the amino terminus of Sam 68 ( including residues 38 45 : PPLPHRSR ) facilitates phosphorylation of Sam 68 by the Src kinase domain . ^^^ |
|