| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The induction of monocytic differentiation by TPA and other agents was further associated with increases in EGR 2 , but not EGR 3 or EGR 4 , mRNA levels in these cells . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The EGR 3 cDNA sequence predicts a 387 amino acid ( a . a . ) protein containing three Cys 2 His2 zinc fingers nearly identical to those of Egr 1 and EGR 2 . ^^^ This similarity has a functional consequence : EGR 3 can activate transcription of a CAT gene linked to the sequence CGCCCCCGC , a cis element which is a target for Egr 1 and EGR 2 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NGFI A ( also called Egr 1 , Zif 268 , or Krox 24 ) and the closely related proteins Krox 20 , NGFI C , and Egr 3 are zinc finger transcription factors encoded by immediate early genes which are induced by a wide variety of extracellular stimuli . ^^^ NAB 1 also represses Krox 20 activity , but it does not influence Egr 3 or NGFI G , thus providing a mechanism for the differential regulation of this family of immediate early transcription factors . . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Okadaic acid also induced the expression of the related immediate early genes Egr 2 and Egr 3 albeit to a lesser extent than Egr 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NGFI C is closely related to the previously described early response genes , nerve growth factor induced clone A ( NGFI A or EGR 1 ) , EGR 2 , and EGR 3 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The zinc finger transcription factor genes Egr 1 , Egr 2 , and Egr 3 were identified . ^^^ Northern blot analyses confirmed that mRNA levels of Egr 1 , Egr 2 , and Egr 3 increased readily after m1AChR stimulation and that a maximum was attained within 50 min . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The WT 1 antibodies do not recognize the structurally and functionally related early growth response ( EGR ) 1 , EGR 2 , EGR 3 , or EGR 4 proteins . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The Egr family of zinc finger transcription factors , whose members include Egr 1 ( NGFI A ) , Egr 2 ( Krox 20 ) , Egr 3 and Egr 4 ( NGFI C ) , are thought to regulate critical genetic programs involved in cellular growth and differentiation ( refs 4 8 , and W . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Analysis of related zinc finger expression in serial sections revealed that NGFI C is expressed in a distinct but overlapping cell populations relative to NGFI A , Krox 20 , and Egr 3 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NGFI A is the prototypic member of a family of immediate early gene encoded transcription factors which includes NGFI C , Egr 3 , and Krox 20 . ^^^ In contrast to the similarities in binding specificity among these proteins we found that while NGFI A , Egr 3 , and Krox 20 have comparable DNA binding affinities and kinetics of dissociation , the affinity of NGFI C is more than threefold lower . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| To find clues about the mechanism and pathways of PCP action , we studied the effect of systemic PCP administration ( 10 and 50 mg / kg , intraperitoneal ) on the expression of immediate early genes ( IEGs ) ( c fos , c jun , egr 2 , egr 3 , NGFI A , NGFI B , NGFI C , and Nurr 1 ) using in situ hybridization histochemistry . ^^^ In contrast , a delayed induction of c fos , c jun , NGFI A , NGFI B , NGFI C , and Nurr 1 in the posterior cingulate cortex was observed 2 6 hr after PCP , 50 mg / kg . egr 2 and egr 3 were not induced in the posterior cingulate cortex . c fos induction in the cerebellar Purkinje cell layer peaked 2 hr after PCP , 50 mg / kg . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| We therefore examined the effect of 5 and 10 min of global ischemia on the expression of the IEGs NGFI A , NGFI B , NGFI C , egr 2 , egr 3 , and Nurr 1 in gerbil brain . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Supershift analysis revealed these proteins to be Egr 1 and Egr 2 ( Krox 20 ) ; Egr 3 was not detected . ^^^ As with egr 3 , expression of egr 2 was blocked by cyclosporin A . ^^^ Although overexpressed Egr 1 was ineffective , overexpressed Egr 2 was as potent as Egr 3 in inducing fasL promoter dependent reporter constructs in T cell hybridomas and HeLa cells , and both up regulated endogenous fasL mRNA in HeLa cells . ^^^ Thus , Egr 2 , in addition to Egr 3 , regulates FasL expression in activated normal T cells , and Egr 2 is likely to play a direct role in aberrant fasL up regulation in lpr / lpr and gld / gld CD 4 ( ) CD 8 ( ) T cells . . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| In the present study in situ hybridization was used to study the effect of kainic acid induced seizures on the expression of the zinc finger immediate early genes ( IEGs ) NGFI A , NGFI B , NGFI C , egr 2 ; egr 3 and Nurr 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| A similar pattern of expression is found for family members Egr 2 and Egr 3 . ^^^ In contrast , the calcineurin inhibitor cyclosporin A , which inhibits DPK cell differentiation as well as positive selection , inhibits expression of Egr 2 and Egr 3 , but not Egr 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NGFI A , NGFI B , NGFI C , egr 2 , egr 3 , and Nurr 1 mRNA were all induced throughout the ipsilateral cortex at 1 hour to 12 hours after MCAO . ^^^ The prominent induction of NGFI B , NGFI C , egr 2 , and egr 3 in the anterior cingulate cortex , the ACA / MCA transition zone , and medial striatum could reflect the ischemic regions around MCA infarcts . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The Egr proteins , Egr 1 , Egr 2 , Egr 3 and Egr 4 , are closely related members of a subclass of immediate early gene encoded , inducible transcription factors . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Bands composed , at least in part , of Egr 2 and Egr 3 were present in different relative amounts in the cerebral cortex , striatum , hippocampus , thalamus , and midbrain . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Nab proteins constitute an evolutionarily conserved family of corepressors that specifically interact with and repress transcription mediated by three members of the NGFI A ( Egr 1 , Krox 24 , zif / 268 ) family of immediate early gene transcription factors , which includes NGFI C , Krox 20 , and Egr 3 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The Egr family of zinc finger transcription factors , whose members are encoded by Egr 1 ( NGFI A ) , Egr 2 ( Krox 20 ) , Egr 3 , and Egr 4 ( NGFI C ) regulate critical genetic programs involved in cellular growth , differentiation , and function . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| To test their roles in cell physiology , we constructed adenoviral recombinants encoding NGFI A binding protein 2 ( NAB 2 , a repressor of EGR 1 , EGR 2 , and EGR 3 ) , EGR 1 , NAB insensitive EGR 1 ( I293F ) ( EGR1 * ) , EGR 2 , and the NAB binding , repressive domain 1 ( R 1 ) of EGR 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Transcriptional control of the expression of Fas ligand has been attributed to a number of factors , including early growth response gene 2 ( Egr 2 ) , Egr 3 , Sp 1 , and NF AT , although a direct contribution of NF AT is controversial . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| One consisted of EGR 1 , EGR 2 , EGR 3 and EGR 4 , whereas the only known paralogue of the other is WT 1 , which controls the developmental fate of the entire nephric system , and therefore of gonads . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| These represented 10 distinct immediate early genes that were up regulated by m1AChR activation : Egr 1 , Egr 2 , Egr 3 , NGFi B , ETR 101 , c jun , jun D , Gos 3 and hcyr 61 , as well as the unknown gene Gig 2 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| One consisted of EGR 1 , EGR 2 , EGR 3 and EGR 4 , whereas the only known paralogue of the other is WT 1 , which controls the developmental fate of the entire nephric system , and therefore of gonads . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The concentrations of mRNAs encoding four secreted proteins ( albumin , transferrin , fibrinogen , and apo A 1 ) , five transcription factors , early growth response factor 1 ( EGRF 1 ) , HNF 4 , NGFI C , EGR 3 , and Krox 20 relative to two housekeeping genes , beta actin and GAPDH were determined simultaneously using kinetic reverse transcriptase polymerase chain methodology ( kRT PCR ) . ^^^ Krox 20 , EGR 3 and NGF1C were expressed at nearly undetectable levels . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| FasL promoter activation by IL 2 through SP 1 and NFAT but not Egr 2 and Egr 3 . ^^^ These IL 2 treated T cells had high nuclear expression of SP 1 and NFAT , but lacked the Egr 2 and Egr 3 that could be induced by anti CD 3 stimulation and had been implicated in FasL gene activation . ^^^ Furthermore , the nuclei of IL 2 treated cells express SP 1 and NFAT , but not Egr 2 and Egr 3 , for FasL gene activation . . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The transcription factors Egr 2 and Egr 3 are potent activators of FasL expression . ^^^ Here we find that Egr 2 and Egr 3 are NFAT target genes . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| To investigate whether one of the major immediate early gene families is involved in the learning and memory of fear , we examined the effects of fear conditioning on the expression of the four members of the early growth response ( EGR ) gene family , EGR 1 , EGR 2 , EGR 3 , and EGR 4 . ^^^ In contrast , the expression of EGR 2 , EGR 3 , and EGR 4 messenger RNA was not increased in the amygdala , hippocampus or cortex following fear conditioning . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Of these , Down syndrome candidate region 1 ( maximum induction = 6 . 1 fold ) was the most profoundly induced , followed by Mifl ( KIAA 0025 ; 5 . 5 fold ) , COX 2 ( 4 . 7 fold ) , EGR 3 ( 3 . 7 fold ) , EGR 2 ( 3 . 2 fold ) , bactericidal / permeability increasing protein ( 3 . 1 fold ) , and CD1B antigen , b polypeptide ( 3 . 1 fold ) . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , we developed phosphorothioate antisense oligonucleotides that efficiently inhibit Egr 1 expression without altering the expression of other family members Egr 2 , Egr 3 and Egr 4 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| These included the transcription factors Egr 1 , Egr 2 , Egr 3 , c Jun , Jun D and Gos 3 ; the growth regulator hCyr 61 ; the signalling factors NGFi B ( nerve growth factor induced gene B ) and Etr 101 ; the unknown gene Gig 2 ( for G protein coupled receptor induced gene 2 ) ; and the acetylcholinesterase gene ( ACHE ) . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Expression of egr 1 ( krox 24 ) , egr 2 ( krox 20 ) , egr 3 , c jun , junB , c fos , fosB , fra 1 , fra 2 , and CREB genes was analyzed by reverse transcription polymerase chain reaction ( RT PCR ) in Schwann cells isolated from neonatal rat sciatic nerves and in the cell lines MSC 80 ( mouse Schwann cells ) , NIH 3T3 ( mouse fibroblasts ) , and CHO ( Chinese hamster ovary cells ) . ^^^ T 3 triggered a rapid ( 15 30 min ) , transient ( 1 2 h ) and strong ( 6 to 15 fold ) stimulation of Egr 1 , Egr 2 , Egr 3 , Jun B , c Fos , and Fos B mRNA expression in Schwann cells . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| There was no apparent compensation among egr 2 , egr 3 , or c fos mRNA and protein expression in the visual cortex of egr 1 KO mice . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| We investigated by RT PCR whether PROG activated expression of transcription factors : Egr 1 ( Krox 24 ) Egr 2 ( Krox 20 ) , Egr 3 , c jun , jun B , jun D , c Fos , Fos B , Fra 1 , Fra 2 , CREB , ATF 4 , SCIP and Sox 10 in cultured Schwann cells . ^^^ PROG triggered a quick ( visible as soon as 15 min ) , strong ( 6 to 18 fold ) and transient ( 1 2 h ) stimulation of Egr 1 , Egr 2 , Egr 3 and Fos B genes expression . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Regulation of Egr transcription factors during chronic cocaine treatment appears to differ from that of the AP 1 transcription factor , since Egr 1 and Egr 3 were induced after both acute and repeated cocaine administration , and that neither Egr 2 nor Egr 3 substituted for Egr 1 after chronic cocaine treatment . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| This could be accounted for by GILZ mediated inhibition of Egr 2 and Egr 3 , NFAT / AP 1 inducible transcription factors that bind a regulatory element in the FasL promoter and up regulate FasL expression . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The Egr family of zinc finger transcription factors , consisting of Egr 1 , Egr 2 , Egr 3 , and Egr 4 , are involved in cellular growth and differentiation . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Specific Egr binding sites have been identified in the 5 ' promoter region of the FasL gene , and Egr 1 , Egr 2 , and Egr 3 mRNA in IEC from mice treated with SEB and from transgenic OT 2 mice after administration of antigen was upregulated . ^^^ Overexpression of Egr 2 and Egr 3 induced endogenous ligand upregulation that was inhibited by overexpression of Egr specific inhibitor Nab 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Mammalian two hybrid assays indicated that Ddx 20 can interact with Egr 1 , Egr 3 , and Egr 4 , in addition to Egr 2 , making it the only known cofactor that interacts with all four Egr family members . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The Egr 1 gene belongs to a family of Egr genes that includes EGR 1 , EGR 2 , EGR 3 , EGR 4 , EGR alpha and the tumor suppressor , Wilms ' tumor gene product , WT 1 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Transient expression of antisense Egr 2 and antisense Egr 3 abolished expression of FasL , which further confirmed the role of Egr in the HBx mediated FasL expression . ^^^ Also we observed that HBx increased the transcriptional activity of Egr 2 and Egr 3 by enhancing expression as well as the transactivation function of these proteins . ^^^ HBx interacted with Egr 2 and Egr 3 in vivo and enhanced binding of Egr to the co activator , cAMP response element binding protein binding protein , which may explain the molecular mechanism by which HBx induced the transactivation function of Egr . ^^^ Taken together , our results show a novel mechanism by which HBx induces FasL gene expression that is mediated by enhancing transcriptional activity of Egr 2 and Egr 3 . . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| Egr 2 and Egr 3 are negative regulators of T cell activation . ^^^ Using microarray analysis we have identified here early growth response gene 2 ( Egr 2 ) and Egr 3 as key negative regulators of T cell activation . ^^^ Overexpression of Egr 2 and Egr 3 was associated with an increase in the E 3 ubiquitin ligase Cbl b and inhibition of T cell activation . ^^^ These data support the idea that Egr 2 and Egr 3 are involved in promoting a T cell receptor induced negative regulatory genetic program . . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| The PILOT protein has a calculated M ( r ) of 42 . 6 kDa and contains three zinc fingers of the C2H2 type at the carboxyl terminus which are highly homologous to the zinc finger regions of the transcription factors EGR 1 , EGR 2 , and pAT 133 . ^^^ |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q06889 and P11161 |
Pubmed |
SVM Score :0.0 |
| NA |
|