Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
DMC 1 is homologous to yeast ' s RAD 51 gene , supporting the view that mitotic DSB repair has been recruited for use in meiotic chromosome metabolism . . ^^^ DMC 1 : a meiosis specific yeast homolog of E . coli recA required for recombination , synaptonemal complex formation , and cell cycle progression . ^^^ Dmc 1 protein is structurally similar to bacterial RecA proteins . dmc 1 mutants are defective in reciprocal recombination , accumulate double strand break ( DSB ) recombination intermediates , fail to form normal synaptonemal complex ( SC ) , and arrest late in meiotic prophase . dmc 1 phenotypes are consistent with a functional relationship between Dmc 1 and RecA , and thus eukaryotic and prokaryotic mechanisms for homology recognition and strand exchange may be related . dmc 1 phenotypes provide further evidence that recombination and SC formation are interrelated processes and are consistent with a requirement for DNA DNA interactions during SC formation . dmc 1 mutations confer prophase arrest . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Rad 51 protein is also homologous to a meiosis specific Dmc 1 protein of S . cerevisiae . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The predicted protein product of mei 3 has significant identity to the Rad 51 and Dmc 1 proteins from Saccharomyces cerevisiae . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In an assay of intrachromosomal pop out recombination , the sep 1 , dmc 1 and rad 51 single mutations had only small effects ; however , pop out recombination was virtually eliminated in the sep 1 dmc1 and sep 1 rad51 double mutants , providing evidence for multiple recombination pathways . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
We have isolated a cDNA homologous to the yeast DMC 1 and RAD 51 genes from Drosophila melanogaster . ^^^ The DMC 1 and RAD 51 genes of Saccharomyces cerevisiae are known to play crucial roles during meiosis and during both meiosis and mitosis , respectively , and their gene products are homologous to each other and to the RecA protein of Escherichia coli . ^^^ Sequence analysis of the corresponding genomic DNA fragment showed one short intron existing in the coding region as in the DMC 1 gene , but not in the RAD 51 gene . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
RecA homologs Dmc 1 and Rad 51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis . ^^^ Dmc 1 and Rad 51 , yeast homologs of the E . coli RecA protein , are shown by immunostaining to localize to as many as 64 sites within spread meiotic nuclei . ^^^ Dmc 1 and Rad 51 colocalize and are therefore likely to act together during recombination . ^^^ Despite their similarities , the two proteins have specialized functions : Dmc 1 complexes do not form in rad 51 mutants , while Rad 51 complexes are retained indefinitely in dmc 1 mutants . ^^^ Dmc 1 and , by inference , Rad 51 form complexes before synapsis as monitored by immunostaining for Zip 1 protein . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Phylogenetic analysis indicated ArLIM 15 to be much closer to meiosis specific LIM 15 and DMC 1 in Saccharomyces cerevisiae than to RAD 51 in S . cerevisiae and its homologues on an evolutionary scale . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Previous studies have shown that the RAD 51 and DMC 1 genes of Saccharomyces cerevisiae encode homologs of the Escherichia coli RecA strand exchange enzyme . ^^^ Results presented here demonstrate that the dmc 1 and rad 51 mutants undergo nearly complete chromosome synapsis , but synaptonemal complex formation is delayed substantially compared with wild type . ^^^ In the zip 1 mutant , chromosomes are paired homologously , but not synapsed , and the protein backbones ( axial elements ) of each pair of chromosomes are connected intimately to each other at a few sites referred to herein as axial associations . dmc 1 zip1 and rad 51 zip1 double mutants assemble axial elements that are not obviously associated , demonstrating that the Dmc 1 and Rad 51 proteins are required to establish or stabilize axial associations . ^^^ We propose that axial associations serve to promote meiotic chromosome synapsis and that the absence of these associations accounts for the delayed and inefficient synapsis observed in dmc 1 and rad 51 strains . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Comparisons of the amino acid sequences of three yeast RecA like proteins , Rad 51 and DMC 1 from S . cerevisiae and Rad 51 from S . pombe , revealed several highly conserved regions . ^^^ The encoded protein shares 68 % and 49 % identical amino acids with the Rad 51 and DMC 1 proteins . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In yeast an Escherichia coli RecA like gene , DMC 1 , is expressed in meiotic prophase and its product co localizes with Rad 51 protein on zygotene chromosomes . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The RecA like proteins Rad 51 and Dmc 1 of yeast are both required for meiotic recombination and the former is also necessary for repair of double strand breaks in vegetative cells . ^^^ This protein exhibits strong sequence homology with the Rad 51 proteins of budding yeast , fission yeast , chickens , mouse and humans , and slightly less ( but still strong ) homology with yeast Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Analysis of the primary sequence of the Rec 2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc 1 , Rad 51 , and Rad 57 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
For the fungal RecA homologs ( S . cerevisiae RAD 51 , RAD 55 , RAD 57 , DMC 1 ; Schizosaccharomyces pombe rad 51 ; Neurospora crassa mei 3 ) a role in homologous recombination and recombinational repair is evident . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The amino acid sequence predicted from the nt sequence showed similarity to the RecA protein of bacteria and the RecA like proteins from yeast : RAD 51 , RAD 57 and DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Analysis of the primary sequence relationships of DMC 1 from Saccharomyces cerevisiae and UvsX of T 4 relative to the three dimensional structure of RecA from Escherichia coli suggests that both proteins are structural homologs of bacterial RecA proteins . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
A like genes from three archaean species with putative protein products similar to Rad 51 and Dmc 1 proteins of the yeast Saccharomyces cerevisiae . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
First , RAD 17 , RAD 24 and MEC 1 are required for the meiotic arrest caused by blocking the repair of DSBs with a mutation in the recA homologue DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In yeast , mutations of this type include rad50S , dmc 1 , rad 51 , and zip 1 . ^^^ DMC 1 , RAD 51 , and ZIP 1 encode two RecA homologs and a synaptonemal complex protein , respectively . ^^^ A red 1 or mek1 / mre4 mutation alleviates completely rad50S , dmc 1 , rad 51 , and zip 1 arrest . ^^^ Finally , the red 1 and mek1 / mre4 mutations appear to alleviate prophase arrest directly rather than by eliminating , or permitting bypass of , the rad50S , dmc 1 , rad 51 , or zip 1 defects . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The deduced 210 amino acid sequence was compared to homologues from bacteria ( RecA ) , eukaryotes ( RAD 51 , DMC 1 ) and archaea ( RadA ) . ^^^ The sequence comparison suggests that Pk REC represents a common prototype of RecA , RAD 51 , DMC 1 and RadA , with higher enzymatic activity . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Dmc 1 consistently evolves faster than Rad 51 , and fungal proteins of both types , especially those of Saccharomyces , change rapidly , particularly in comparison to the slowly evolving vertebrate proteins . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The archaeal sequences branch monophyletically and are most closely related to the eukaryotic paralogous Rad 51 and Dmc 1 groups . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The phenotypes conferred by sae 3 1 are similar to those conferred by mutation of the yeast RecA homologue DMC 1 , suggesting that SAE 3 and DMC 1 act at the same step ( s ) of chromosome metabolism . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
We have examined formation of joint molecule recombination intermediates ( JMs ) between homologs and between sisters in yeast strains lacking the meiotic chromosomal protein Red 1 , the meiotic recA homolog Dmc 1 , and / or mitotic recA homolog ( s ) , Rad 51 , Rad 55 , and Rad 57 . ^^^ Coordinate action of mitotic recA homologs as one functional unit , two functions of RED 1 , and an interhomolog interaction function of DMC 1 are also revealed . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
DMC 1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD 51 pathway . ^^^ Epistasis analysis suggests that DMC 1 and RAD 51 function in separate pathways responsible for meiotic recombination . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
DSBs were repaired in RTG in dmc 1 , but not rad 51 mutants , hence repair did not occur by the usual meiotic mechanism which requires the Dmc 1 gene product . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae recA homologues RAD 51 and DMC 1 have both distinct and overlapping roles in meiotic recombination . ^^^ BACKGROUND : Rad 51 and Dmc 1 are Saccharomyces cerevisiae homologues of the Escherichia coli recombination protein RecA . ^^^ RESULTS : We have further characterized rad 51 and dmc 1 single mutants . ^^^ A dmc 1 mutation confers more severe defects in double strand break ( DSB ) resolution , crossover recombination and meiotic progression than does a rad 51 mutant ; in contrast , during return to growth , a rad 51 mutation confers more severe defects in viability and intrachromosomal recombination than does a dmc 1 mutation . ^^^ Consistent with the observation of DMC 1 dependent recombination in a rad 51 mutant , subnuclear complexes of Dmc 1 protein were detected for the first time in this mutant . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In yeast , the Rad 51 family of related proteins also includes Rad 55 , Rad 57 and Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Isolation and characterisation of the RAD 51 and DMC 1 homologs from Arabidopsis thaliana . ^^^ By using RT PCR and degenerate oligonucleotides based on the sequence homology between the yeast RAD 51 and DMC 1 genes , two genes belonging to the RAD 51 and DMC 1 families were isolated from Arabidopsis thaliana ecotype Columbia . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Consistent with a similar role in mammals , Rad 1 protein is abundant in testis , and is associated with both synapsed and unsynapsed chromosomes during meiotic prophase 1 of spermatogenesis , with a staining pattern distinct from that of the recombination proteins Rad 51 and Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
ATR , a protein related to ATM , DMC 1 , a RAD 51 family member , and RAD 51 are mislocalized to chromatin and have reduced localization to developing synaptonemal complexes in spermatocytes from Atm deficient mice , suggesting dysregulation of the orderly progression of meiotic events . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The amino acid sequences encoded by the PCR fragments , three RadA protein sequences previously published ( 21 ) , and two new complete RadA sequences were aligned with representative bacterial RecA proteins and eucaryal Rad 51 and Dmc 1 proteins . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The human Dmc 1 ( hDmc 1 ) protein , a meiosis specific recombinase , is homologous to RecA . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
For instance , our phylogenetic analysis indicates that archaeal recombinases occur in two paralogous gene families , one of which is very deeply branched , and both recombinases are more closely related to the eukaryotic RAD 51 and Dmc 1 gene families than to the Escherichia coli recA gene . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Checkpoint gene function prevents meiotic progression when recombination is blocked by mutations in the recA homologue DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Escherichia coli recA and its yeast homologs RAD 51 and DMC 1 play crucial roles in mitotic and / or meiotic recombination and in repair of double strand DNA breaks . ^^^ The predicted 329 amino acid protein showed significant homology to mouse Rec 2 , Rad 51 , Dmc 1 ( or Lim 15 ) and E . coli RecA . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc 1 , a germline specific RecA homolog . ^^^ DMC 1 is a meiosis specific gene first discovered in yeast that encodes a protein with homology to RecA and may be component of recombination nodules . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The mouse RecA like gene Dmc 1 is required for homologous chromosome synapsis during meiosis . ^^^ The mouse Dmc 1 gene is an E . coli RecA homolog that is specifically expressed in meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Rad 52 and RPA foci are distinct from those formed by Rad 51 , and its meiosis specific relative Dmc 1 , in that they are also detected in meiosis during replication . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Seven members of the Rad 51 family have been identified in vertebrate cells , including Rad 51 , Dmc 1 , and five Rad 51 related proteins referred to as Rad 51 paralogs , which share 20 to 30 % sequence identity with Rad 51 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Tid1 / Rdh54 promotes colocalization of rad 51 and dmc 1 during meiotic recombination . ^^^ Two RecA homologs , Rad 51 and Dmc 1 , assemble as cytologically visible complexes ( foci ) at the same sites on meiotic chromosomes . ^^^ Previous studies suggested that normal Dmc 1 loading depends on Rad 51 . ^^^ The role of Tid1 / Rdh54 in coordinating RecA homolog assembly may be very direct , because Tid1 / Rdh54 is known to physically bind both Dmc 1 and Rad 51 . ^^^ Thus , Tid 1 may normally act with Rad 51 to promote ordered RecA homolog assembly by blocking Dmc 1 until Rad 51 is present . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The Ce rdh 1 gene [ RAD 51 , DMC 1 ( LIM 15 ) , homolog 1 or Ce rad 51 ] , which is essential for the meiotic recombination , is the only bacterial recA like gene in the nematode genome , and is strongly expressed in the meiotic cells . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
RAD 51 and DMC 1 form mixed complexes associated with mouse meiotic chromosome cores and synaptonemal complexes . ^^^ The eukaryotic RecA homologues RAD 51 and DMC 1 function in homology recognition and formation of joint molecule recombination intermediates during yeast meiosis . ^^^ With antibodies that have been immunodepleted of cross reactive epitopes , we demonstrate that RAD 51 and DMC 1 have identical distribution patterns in extracts of mouse spermatocytes in successive prophase 1 stages , suggesting coordinate functionality . ^^^ We also show that mouse RAD 51 and DMC 1 establish protein protein interactions with each other and with the chromosome core component COR 1 ( SCP 3 ) in a two hybrid system and in vitro binding analyses . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
BACKGROUND : DMC 1 , the meiosis specific eukaryotic homologue of bacterial recA , is required for completion of meiotic recombination and cell cycle progression past prophase . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Eukaryotic cells encode two homologs of Escherichia coli RecA protein , Rad 51 and Dmc 1 , which are required for meiotic recombination . ^^^ In contrast to helical RecA / Rad51 filaments , however , Dmc 1 filaments are composed of a linear array of stacked protein rings . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Based on this finding , we screened for a meiosis specific homolog of recA , equivalent to Lilium LIM 15 or Saccharomyces DMC 1 , in C . cinereus , and isolated a clone containing a 1 . 2 kb DNA fragment from a cDNA library constructed with Coprinus poly ( A ) + RNA isolated from cells undergoing meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
BLM protein expression and location are detected by immunofluorescence and immunoelectron microscopy as discrete foci that are sparsely present on early meiotic prophase chromosome cores , later found abundantly on synapsed cores , frequently in combination with the recombinases RAD 51 and DMC 1 , and eventually as pure BLM foci . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
DNA homology recognition in these instances often involves the ( RecA ) homolog RAD 51 and / or the related gene , DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
DMC 1 is a specialized homolog of the bacterial RecA protein , whereas the SPO 11 gene is of archaebacterial origin . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Recombination is also modulated by isochore determinants : R bands differentially favor double strand break ( DSB ) formation but disfavor subsequent loading of meiotic RecA homolog , Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The Rad 51 and Dmc 1 recombinases : a non identical twin relationship . ^^^ This review summarizes the key roles played by the two recombinases , Dmc 1 and Rad 51 , in the faithful repair of DNA breaks . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The RecA homolog DMC 1 from yeast and its orthologs from other species have been reported to be meiosis specific and essential for sporulation . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Human Dmc 1 protein , a meiosis specific homolog of Escherichia coli RecA protein , has previously been shown to promote DNA homologous pairing and strand exchange reactions that are qualitatively similar to those of RecA protein and Rad 51 . ^^^ The effects of helix stability and mismatched base pairs on the recognition of homology revealed further that human Dmc 1 , like human Rad 51 , requires the preferential breathing of A small middle dotT base pairs for recognition of homology . ^^^ We conclude that Dmc 1 , like human Rad 51 and E . coli RecA protein , promotes homologous pairing and strand exchange by a `` synaptic pathway ' ' involving a three stranded nucleoprotein intermediate , rather than by a `` helicase pathway ' ' involving the separation and reannealing of DNA strands . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Dmc 1 and Rad 51 are eukaryotic RecA homologues that are involved in meiotic recombination . ^^^ The expression of Dmc 1 is limited to meiosis , whereas Rad 51 is expressed in mitosis and meiosis . ^^^ Dmc 1 and Rad 51 have unique and overlapping functions during meiotic recombination . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Sequence analysis and biochemical studies have shown that archaeal RadA proteins are more closely related to Rad 51 and Dmc 1 than the bacterial RecA proteins . ^^^ Since it is likely that RadA is closely related to a common ancestral protein of both Rad 51 and Dmc 1 , the two DNA binding forms of RadA may provide insight into the divergence that has taken place between Rad 51 and Dmc1 . . ^^^ Surprisingly , the human meiosis specific homolog of RecA , Dmc 1 , has thus far only been observed to bind DNA as an octameric ring . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Tid1 / Rdh54 interacts with yeast Rad 51 and a meiosis specific Rad 51 homolog , Dmc 1 . ^^^ In contrast , neither human Rad 51 nor Dmc 1 directly interacts with hRad54B . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
RAD 51 and / or DMC 1 coimmunoprecipitates with REC 8 , suggesting that REC 8 may also provide a basis for assembly of recombination complexes . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Two RecA like recombinases , Rad 51 and Dmc 1 , function together during double strand break ( DSB ) mediated meiotic recombination to promote homologous strand invasion in the budding yeast Saccharomyces cerevisiae . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The immunocytochemical localization of Dmc 1 and Rad 51 supports the idea that these proteins are not involved in homology search and final pairing . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
These mutations also decrease the colocalization of immunostaining foci of the RecA homologs Rad 51 and Dmc 1 and cause a delay in the disappearance of Rad 51 foci , but not of Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Genetic and cytological characterization of the RecA homologous proteins Rad 51 and Dmc 1 of Schizosaccharomyces pombe . ^^^ The Schizosaccharomyces pombe rad 51 ( + ) and dmc 1 ( + ) genes code for homologues of the Escherichia coli recombination protein RecA . ^^^ We describe the functional defects of terminally epitope tagged Dmc 1 and Rad 51 and discuss it in terms of protein interaction . ^^^ Presumptive Rad 51 and Dmc 1 foci were detected on spreads of meiotic chromatin . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Similarly , human RAD 51 paralogues , including RAD51B , RAD51C , RAD51D , XRCC 2 , XRCC 3 and DMC 1 , are not predicted to bind the BRC repeat . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Genetic analysis indicates that Hop 2 acts in the same pathway as the Rad 51 and Dmc 1 proteins , two homologs of E . coli RecA . ^^^ Overexpression of RAD 51 largely suppresses the meiotic defects of the dmc 1 and hop 2 mutants . ^^^ We conclude that Rad 51 is capable of carrying out a homology search independently , whereas Dmc 1 requires additional factors such as Hop2 . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Spore viability was reduced in rad 51 , rhp 55 , and rhp 57 mutants , but not in rlp 1 and dmc 1 . ^^^ Analysis of double and triple mutants revealed that Rad 51 and Dmc 1 play major and partially overlapping roles in meiotic recombination , while Rhp 55 , Rhp 57 , and Rlp 1 play accessory roles . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Rad 51 like proteins have been identified from yeast ( Rad 55 , Rad 57 and Dmc 1 ) to vertebrates ( Rad51B , Rad51C , Rad51D , Xrcc 2 , Xrcc 3 and Dmc 1 ) . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In a yeast two hybrid assay , the Arabidopsis Brca 2 proteins interact with Rad 51 and Dmc 1 . ^^^ We obtained the same phenotype upon introduction of RNAi constructs aimed at silencing the RAD 51 gene at meiosis in dmc 1 mutant plants . ^^^ We also propose a role for Rad 51 in the dmc 1 context . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
This progression may relate to compensatory gene function ( s ) and / or the observed up regulation of DNA repair proteins Rad 51 and Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
FANCD 2 did not interact with RAD 51 , the five RAD 51 paralogs , RAD 52 , RAD 54 or DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
RecA and Rad 51 form helical filaments , while Dmc 1 forms an octameric ring . ^^^ The monomeric structure of the Dmc 1 protein closely resembled those of the human and archaeal Rad 51 proteins . ^^^ In addition to the polymerization motif that was previously identified in the Rad 51 proteins , we found another hydrogen bonding interaction at the polymer interface , which could explain why Dmc 1 forms stable octameric rings instead of helical filaments . ^^^ The human Dmc 1 protein , a RecA / Rad51 homolog , is a meiosis specific DNA recombinase that catalyzes homologous pairing . ^^^ RecA and Rad 51 form helical filaments , while Dmc 1 forms an octameric ring . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Two RecA like recombinases , Rad 51 and Dmc 1 , exist in eukaryotes . ^^^ Whereas Rad 51 is needed for both mitotic and meiotic recombination events , the function of Dmc 1 is restricted to meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Genetic analysis shows that Mcp 7 acts in the downstream of Dmc 1 , homologs of Escherichia coli RecA protein , for both recombination and subsequent sporulation . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The process is facilitated by a superfamily of recombinases : bacterial RecA , archaeal RadA and Rad 51 , and eukaryal Rad 51 and DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In this review , we discuss primarily those proteins involved in the initial stages of homologous recombination , including SPO 11 , MRE 11 , RAD 50 , NBS 1 , DMC 1 , RAD 51 , RAD 51 paralogs , RAD 52 , RPA , RAD 54 , and RAD54B . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Roles of RecA homologues Rad 51 and Dmc 1 during meiotic recombination . ^^^ Both Rad 51 and Dmc 1 are necessary to generate a crossover between homologous chromosomes , which ensures the segregation of the chromosomes at meiotic division 1 . ^^^ In this review , recent advances on our knowledge about the roles of Rad 51 and Dmc 1 during meiosis are summarized and discussed . . ^^^ Additionally , meiotic cells induce the second RecA , Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Association between MSH 4 ( MutS homologue 4 ) and the DNA strand exchange RAD 51 and DMC 1 proteins during mammalian meiosis . ^^^ Our results provide biochemical evidence indicating that the human MSH 4 protein physically interacts with both RAD 51 and DMC 1 , two RecA homologues known to initiate DNA strand exchange between homologous chromosomes . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Meiotic recombination in eukaryotic cells requires two homologs of E . coli RecA protein , Rad 51 and Dmc 1 . ^^^ Until recently , the role of Dmc 1 in meiotic recombination was mostly attributed to genetic studies as purified Dmc 1 was found to be a much weaker recombinase than Rad 51 in the test tube . ^^^ Now , Sehorn and colleagues 1 have reported that , like Rad 51 , human Dmc 1 is an efficient recombinase in vitro . ^^^ Dmc 1 forms helical nucleoprotein filaments the signature of classical recombinases such as Rad 51 . ^^^ These observations reveal a high level of similitude between the Dmc 1 and the Rad 51 family of recombination enzymes in higher eukaryotes . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
At the amino acid sequence level , archaeal RadA and Rad 51 and eukaryal Rad 51 and meiosis specific DMC 1 form a closely related group of recombinases distinct from bacterial RecA . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Meiotic recombination requires the meiosis specific RecA homolog Dmc 1 as well as the mitotic RecA homolog Rad 51 . ^^^ Here , we show that the two meiosis specific proteins Mei 5 and Sae 3 are necessary for the assembly of Dmc 1 , but not for Rad 51 , on chromosomes including the association of Dmc 1 with a recombination hot spot . ^^^ Mei 5 , Sae 3 , and Dmc 1 form a ternary and evolutionary conserved complex that requires Rad 51 for recruitment to chromosomes . ^^^ Mei 5 , Sae 3 , and Dmc 1 are mutually dependent for their chromosome association , and their absence prevents the disassembly of Rad 51 filaments . ^^^ Our results suggest that Mei 5 and Sae 3 are loading factors for the Dmc 1 recombinase and that the Dmc 1 Mei5 Sae 3 complex is integrated onto Rad 51 ensembles and , together with Rad 51 , plays both catalytic and structural roles in interhomolog recombination during meiosis . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Recent studies in budding and fission yeasts have revealed Mei 5 and Sae 3 as factors necessary for the proper function of the recombinases Dmc 1 and Rad 51 in DNA repair and meiotic recombination , providing new insights into how strand exchange proteins are directed along specific recombination pathways . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The Hop 2 and Mnd 1 proteins act in concert with Rad 51 and Dmc 1 in meiotic recombination . ^^^ Rad 51 and Dmc 1 recombinases are the major players in these processes . ^^^ Furthermore , the Hop 2 Mnd1 heterodimer physically interacts with Rad 51 and Dmc 1 recombinases and stimulates their activity up to 35 fold . ^^^ Our data reveal an interplay among Hop 2 , Mnd 1 and Rad 51 and Dmc 1 in the formation of the first recombination intermediates during meiosis . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae Dmc 1 , a meiosis specific homologue of RecA , catalyzes homologous pairing and strand exchange during meiotic DNA recombination . ^^^ The purified budding yeast Dmc 1 ( ScDmc 1 ) protein exhibits much weaker recombinase activity in vitro as compared to that of the Escherichia coli RecA protein . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Fission yeast rad 51 and dmc 1 , two efficient DNA recombinases forming helical nucleoprotein filaments . ^^^ Eukaryotic cells require two homologs of Escherichia coli RecA protein , Rad 51 and Dmc 1 , for meiotic recombination . ^^^ To gain insight into this , we purified Schizosaccharomyces pombe Rad 51 and Dmc 1 to homogeneity . ^^^ Purified Rad 51 and Dmc 1 form homo oligomers , bind single stranded DNA preferentially , and exhibit DNA stimulated ATPase activity . ^^^ Both Rad 51 and Dmc 1 promote the renaturation of complementary single stranded DNA . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In mammals , 7 recA like genes have been identified : RAD 51 , RAD51L1 / B , RAD51L2 / C , RAD51L3 / D , XRCC 2 , XRCC 3 , and DMC 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The DMC 1 protein , a eukaryotic homologue of RecA that shares significant amino acid identity with RAD 51 , exhibits two oligomeric DNA binding forms , an octameric ring and a helical filament . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Rad 51 and its meiotic homolog Dmc 1 are key proteins of homologous recombination in eukaryotes . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
A novel RecA like protein , differing from Dmc 1 and Rad 51 , was characterized in Oryza sativa L . cv . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
This finding suggests that the number or protomers per helical turn and / or the number of nucleotides bound per Dmc 1 protomer differs from that reported previously for Rad 51 and RecA filaments . ^^^ These effects , together with previous studies of other RecA like recombinases , support the view that ATP binding to Dmc 1 protomers is required for functional filament structure . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Archaeal RadA and Rad 51 and eukaryal Rad 51 and meiosis specific DMC 1 form a closely related group of recombinases distinct from bacterial RecA . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In mammals , HR requires the activities of the RAD 51 family ( RAD 51 , RAD51B , RAD51C , RAD51D , XRCC 2 , XRCC 3 and DMC 1 ) , each of which contains conserved ATP binding sequences ( Walker Motifs A and B ) . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Here , we show that T . brucei , Trypanosoma cruzi and Leishmania major each contain a single copy gene whose product is highly related to the eukaryotic meiosis specific protein Dmc 1 , which is structurally and functionally related to Rad 51 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Here we report that EZH 2 decreased the expression of five RAD 51 paralog proteins involved in homologous recombination ( HR ) repair of DNA double strand breaks ( RAD51B / RAD51L1 , RAD51C / RAD51L2 , RAD51D / RAD51L3 , XRCC 2 , and XRCC 3 ) , but did not affect the levels of DMC 1 , a gene that only functions in meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The Rad 51 and Dmc 1 proteins , the eukaryotic RecA orthologs , promote strand exchange and may function in the extensive matching of homology within paired DNA molecules . ^^^ In the present study , we purified the human TBPIP / Hop2 Mnd 1 complex and found that it significantly stimulates the Dmc 1 and Rad 51 mediated strand exchange . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The human Rad 51 and Dmc 1 proteins themselves can interact with either the complete ( HsRad 51 ) or a shorter version of AtBrca 2 ( HsRad 51 or HsDmc 1 ) that comprises all four BRC motifs . ^^^ Interaction between Arabidopsis Brca 2 and its partners Rad 51 , Dmc 1 , and Dss 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Recombination in meiosis in Saccharomyces cerevisiae relies on two Escherichia coli recA homologs , Rad 51 and Dmc 1 , as well as the more recently discovered heterodimer Mnd1 / Hop2 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The mouse Hop 2 and Mnd 1 proteins , which can form a stable heterodimeric complex , ensure the proper synapsis of homologous chromosomes in meiosis by acting in concert with Rad 51 and Dmc 1 to promote the strand invasion ( D loop formation ) step of homologous recombination . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Rad 51 and disrupted meiotic cDNA 1 ( Dmc 1 ) are the two eukaryotic DNA recombinases that participate in homology search and strand exchange reactions during homologous recombination mediated DNA repair . ^^^ Rad 51 expresses in both mitotic and meiotic tissues whereas Dmc 1 is confined to meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Processing of these sites of DSBs requires the function of RecA homologs , such as RAD 51 , DMC 1 , and others , as suggested by mutant studies ; thus the failure to repair these meiotic DSBs results in abnormal chromosomal alternations , leading to disrupted meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
However , many archaeal species have two recA / RAD51 homologs ( RADA and RADB ) , and eukaryotes possess multiple members ( RAD 51 , RAD51B , RAD51C , RAD51D , DMC 1 , XRCC 2 , XRCC 3 , and recA ) . ^^^ In the RADalpha subfamily , eukaryotic RAD 51 and DMC 1 genes formed two separate monophyletic groups when archaeal RADA genes were used as an outgroup . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
In budding yeast , there are two RecA homologs : Rad 51 and Dmc 1 . ^^^ While Rad 51 is involved in both mitotic and meiotic recombination , Dmc 1 participates specifically in meiotic recombination . ^^^ Several observations indicate that Hed 1 attenuates Rad 51 activity when Dmc 1 is absent . ^^^ First , although double strand breaks are normally poorly repaired in the dmc 1 mutant , repair becomes efficient when Hed 1 is absent , and this effect depends on Rad 51 . ^^^ We propose that Hed 1 provides a mechanism to ensure the coordinated action of Rad 51 and Dmc 1 during meiosis , by down regulating Rad 51 activity when Dmc 1 is unavailable . . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Red Hed regulation : recombinase Rad 51 , though capable of playing the leading role , may be relegated to supporting Dmc 1 in budding yeast meiosis . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
This interaction requires the strand exchange proteins Rad 51 and Dmc 1 that bind to single stranded regions created by resection of ends at the site of DSBs and promote interactions with uncut DNA on the homologous partner . ^^^ In budding yeast Hop 2 and Mnd 1 act as a complex to promote homologous pairing and recombination in conjunction with Rad 51 and Dmc 1 . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
Nucleoprotein filaments made up of Rad 51 or Dmc 1 recombinases , the core structures of recombination , engage in ATP dependent DNA strand exchange . ^^^ Here , we show that the Schizosaccharomyces pombe Swi 5 Sfr1 complex , a conserved eukaryotic protein complex , at substoichiometric concentrations stimulates strand exchange mediated by Rhp 51 ( the S . pombe Rad 51 homolog ) and Dmc 1 on long DNA substrates . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
The human Rad54B protein , which belongs to the SWI2 / SNF2 protein family , plays a role in homologous recombination , and may function with the Dmc 1 recombinase , a meiosis specific Rad 51 homolog . ^^^
Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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Interacting proteins: Q8IV36 and Q06609	Pubmed	SVM Score :0.0
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