| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.76064014 |
| Our results suggest that BRCA 2 can interact with RAD 51 through multiple sites of BRCA 2 and that control of mitotic and meiotic recombination and / or of genomic integrity through binding to RAD 51 may be a crucial mechanism by which BRCA 2 suppresses abnormal proliferation of mammary cells . . 0.76064014^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.50827835 |
| Complex regulation of HRR by cell cycle checkpoint and surveillance functions is suggested not only by direct interactions between human Rad 51 and p 53 , c Abl , and BRCA 2 , but also by very high recombination rates in p 53 deficient cells . . 0.50827835^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.59666439 |
| Human Rad 51 ( hRad 51 ) has been found to be associated with BRCA 1 , BRCA 2 , and p 53 either directly or indirectly and is one of at least eight human genes that are members of the Escherichia coli RecA / Saccharomyces cerevisiae Rad 51 family thought to affect genomic stability through DNA recombination / repair processes . 0.59666439^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.53213678 |
| BRCA 2 binds to Rad 51 through its BRC repeats . 0.53213678^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.97661861 |
| Exon 11 of murine Brca 2 is required for its stable association with RAD 51 , whereas the carboxyl terminus of Brca 2 is dispensable for this interaction . 0.97661861^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.77179539 |
| We deleted this Brca 2 COOH terminal domain because it interacts directly with the Rad 51 protein , contains a nuclear localization signal , and is required to maintain genomic stability in response to various types of DNA damage . 0.77179539^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.550291 |
| The breast cancer associated BRCA 1 and BRCA 2 proteins are strongly implicated in HRR ; BRCA 2 associates with Rad 51 and appears to regulate its activity . 0.550291^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.623862 |
| Both exons 11 and 27 of Brca 2 code for domains that interact with RAD 51 ; exon 11 encodes eight BRC motifs , whereas exon 27 encodes a single , distinct interaction domain . 0.623862^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.62543466 |
| Here we have used the yeast two hybrid system to test for direct interaction between BRCA 2 or its effector RAD 51 and the FANCA , FANCC and FANCG proteins . 0.62543466^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.69790924 |
| It is known that BRCA 2 interacts directly with RAD 51 through a series of degenerative motifs known as the BRC repeats . 0.69790924^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.7722755 |
| Recent experimental findings shape the current models for BRCA 2 function , and structural and biochemical advances shed new light on the interactions between BRCA 2 , the RAD 51 recombinase and single stranded DNA during DNA recombination . . 0.7722755^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.86845112 |
| This modification blocks C terminal interactions between BRCA 2 and RAD 51 . 0.86845112^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :1.4651048 |
| BRCA 2 has been shown to interact directly with RAD 51 , an essential component of the cellular machinery for homologous recombination and the maintenance of genome stability . 1.4651048^^^ Interactions between BRCA 2 and RAD 51 take place by means of the conserved BRC repeat regions of BRCA 2 . 0.94177497^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.5480962 |
| Whereas BRCA 2 interacts directly with and regulates RAD 51 , the role of BRCA 1 in this process is unclear . 0.5480962^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.53597579 |
| Interaction between Arabidopsis Brca 2 and its partners Rad 51 , Dmc 1 , and Dss 1 . 0.53597579^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Embryonic lethality and radiation hypersensitivity mediated by Rad 51 in mice lacking Brca 2 . ^^^ The embryonic expression pattern of the mouse Brca 2 gene is now defined and an interaction identified of the Brca 2 protein with the DNA repair protein Rad 51 . ^^^ Developmental arrest in Brca 2 deficient embryos , their radiation sensitivity , and the association of Brca 2 with Rad 51 indicate that Brca 2 may be an essential cofactor in the Rad 51 dependent DNA repair of double strand breaks , thereby explaining the tumour suppressor function of Brca2 . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RAB 22 and RAB163 / mouse BRCA 2 : proteins that specifically interact with the RAD 51 protein . ^^^ RAB 163 encodes the C terminal portion of mouse BRCA 2 , the homologue of the second breast cancer susceptibility gene protein in humans , demonstrating an in vitro association between RAD 51 and BRCA 2 . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RAD 51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca 2 . ^^^ Recent work has shown that the murine BRCA 2 tumor suppressor protein interacts with the murine RAD 51 protein . ^^^ Through yeast two hybrid and biochemical assays , we demonstrate that the RAD 51 protein interacts specifically with the eight evolutionarily conserved BRC motifs encoded in exon 11 of brca 2 and with a similar motif found in a Caenorhabditis elegans hypothetical protein . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The interaction of HsRad 51 with several tumor suppressor genes namely p 53 , BRCA 1 and BRCA 2 implies possible role ( s ) of this protein in tumorigenesis . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRC repeats in BRCA 2 are critical for RAD 51 binding and resistance to methyl methanesulfonate treatment . ^^^ Furthermore , direct binding of human RAD 51 to each of the four single 30 amino acid BRC repeats located at the 5 ' portion of exon 11 of BRCA 2 was demonstrated . ^^^ Such an interaction is significant , as BRCA 2 and RAD 51 can be reciprocally coimmunoprecipitated by each of the individual , specific antibodies and form complexes in vivo . ^^^ Inferring from the function of RAD 51 in DNA repair , human pancreatic cancer cells , Capan 1 , expressing truncated BRCA 2 were shown to be hypersensitive to methyl methanesulfonate ( MMS ) treatment . ^^^ These results suggest that the interaction between the BRC repeats of BRCA 2 and RAD 51 is critical for cellular response to DNA damage caused by MMS . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 1 and BRCA 2 account for most cases of familial , early onset breast and / or ovarian cancer and encode products that each interact with hRAD 51 . ^^^ Like BRCA 1 and RAD 51 , BRCA 2 relocates to PCNA+ replication sites following exposure of S phase cells to hydroxyurea or UV irradiation . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 gene product functionally interacts with p 53 and RAD 51 . ^^^ Here we show that the BRCA 2 gene product is a 460 kDa nuclear phosphoprotein , which forms in vivo complexes with both p 53 and RAD 51 . ^^^ Moreover , exogenous BRCA 2 expression in cancer cells inhibits p 53 ' s transcriptional activity , and RAD 51 coexpression enhances BRCA 2 ' s inhibitory effects . ^^^ Thus , BRCA 2 likely participates with p 53 and RAD 51 in maintaining genome integrity . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The studies summarized here suggest that BRCA 1 , BRCA 2 , RAD 51 . and BARD 1 function as a biochemical complex . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Wild type BRCA 1 protein binds to a number of cellular proteins , including DNA repair protein Rad 51 , tumor suppressor p 53 , RNA polymerase 2 holoenzyme , RNA helicase A , CtBP interacting protein , c myc , BRCA 1 associated RING domain protein ( BARD 1 ) , BRCA 2 protein , etc . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The two major hereditary breast cancer susceptibility genes , BRCA 1 and BRCA 2 , are associated with early onset breast and / or ovarian cancer and encode products that each interact with the product of the eukaryotic RecA homologue , hRad 51 . ^^^ BRCA 1 , BRCA 2 , and Rad 51 operate in a common DNA damage response pathway . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Coordinate alterations in the expression of BRCA 1 , BRCA 2 , p 300 , and Rad 51 in response to genotoxic and other stresses in human prostate cancer cells . ^^^ The purpose of this study was to evaluate the effects of genotoxic and other cytotoxic agents on expression of DNA damage response genes ( BRCA 1 , BRCA 2 , p 300 , and Rad 51 ) in human prostate cancer cells . ^^^ BRCA 2 showed a more rapid decrease in protein than mRNA , while Rad 51 showed the opposite . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Association of breast tumor susceptibility gene products BRCA 1 and BRCA 2 with the RAD 51 recombination protein suggested that cancer could arise through defects in recombination . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Association of a recombinational repair protein RAD 51 with tumor suppressors BRCA 1 and BRCA 2 suggests that defects in homologous recombination are responsible for tumor formation . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 is required for ionizing radiation induced assembly of Rad 51 complex in vivo . ^^^ Both BRCA 1 and BRCA 2 have been reported to bind Rad 51 , a protein essential for homologous recombination and the recombinational repair of DNA double strand breaks . ^^^ Here we demonstrate that IR induced Rad 51 foci formation is aberrant in BRCA 2 but not BRCA 1 deficient tumor cells . ^^^ In Capan 1 cells , which do not express functional BRCA 2 , there was little Rad 51 foci formation in response to a wide range of radiation dosages . ^^^ Moreover , forced expression of a fusion protein containing green fluorescent protein and the first Rad 51 binding BRC repeat of BRCA 2 in cells with wild type BRCA 2 rendered them hypersensitive to IR and cisplatin and compromised IR induced Rad 51 foci formation . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The physical interactions among the products of RAD 51 , BRCA 1 , and BRCA 2 have suggested that the BRCA 1 and BRCA 2 breast cancer susceptibility genes may function , at least in part , in this DNA damage repair pathway . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Detection of loss of heterozygosity at RAD 51 , RAD 52 , RAD 54 and BRCA 1 and BRCA 2 loci in breast cancer : pathological correlations . ^^^ Loss of heterozygosity ( LOH ) in loci of the 15q15 . 1 , 12p13 , 1p32 , 17q21 and 13q12 13 regions may collaborate in the inactivation of RAD 51 , RAD 52 , RAD 54 , BRCA 1 , BRCA 2 and possibly other genes implicated in the repair of double stranded DNA and in DNA recombination . ^^^ We investigate allelic losses in microsatellites of the RAD 51 , RAD 52 , RAD 54 , BRCA 1 and BRCA 2 regions , and their correlations with nine pathologic parameters in 127 breast carcinomas . ^^^ LOH was found in the RAD 51 region in 32 % of tumours , in the RAD 52 region in 16 % , in RAD 54 in 20 % and in the BRCA 1 and BRCA 2 regions in 49 % and 44 % respectively . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Whereas most mutations in BRCA 1 and BRCA 2 are known to affect the more carboxy terminal regions interacting with RAD 51 , and the transactivating BRCT domains of BRCA 1 , this is the first demonstration of a recurrent BRCA 2 mutation that specifically deletes the amino terminal transactivation domain . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In alpha particle irradiated cells and in those cells subsequently cultured in the presence of E , increased BRCA 1 , BRCA 2 and RAD 51 expression were detected by immunofluorescence staining and quantified by confocal microscopy . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 1 and BRCA 2 proteins form a complex with Rad 51 , and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double strand break repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 1 contains several functional domains that interact directly or indirectly with a variety of molecules , including tumor suppressors ( p 53 , RB , BRCA 2 and ATM ) , oncogenes ( c Myc , casein kinase 2 and E2F ) , DNA damage repair proteins ( RAD 50 and RAD 51 ) , cell cycle regulators ( cyclins and cyclin dependent kinases ) , transcriptional activators and repressors ( RNA polymerase 2 , RHA , histone deacetylase complex and CtIP ) and others . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In particular , the sensitivity of BRCA 2 deficient mouse embryonic fibroblasts to ionizing radiation and the demonstrated interaction of the BRCA 2 protein with Rad 51 , a major factor in recombinational repair , indicate that BRCA 2 is important for double strand break repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The functions of the proteins encoded by BRCA 1 and BRCA 2 remain to be elucidated , but their interaction and colocalization with hRAD 51 suggest a role in homologous recombination and DNA double strand break ( DSB ) repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 keeps Rad 51 in line . ^^^ The mechanistic link between the BRCA 2 protein , homologous recombination , and genomic instability has become clearer as a result of recent work describing the structures of different BRCA 2 domains bound to Rad 51 and to single stranded DNA . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| A single nucleotide polymorphism in the RAD 51 gene modifies cancer risk in BRCA 2 but not BRCA 1 carriers . ^^^ The BRCA 1 and BRCA 2 proteins function in DNA repair in conjunction with RAD 51 . ^^^ A preliminary report suggested that a single nucleotide polymorphism in the 5 ' untranslated region of RAD 51 ( 135C / G ) increases breast cancer risk in BRCA 1 and BRCA 2 carriers . ^^^ However , in BRCA 2 carriers , RAD 51 135C heterozygote frequency in affected women was 17 . 4 % ( 8 of 46 ) compared with 4 . 9 % ( 2 of 41 ) in unaffected women ( P = 0 . 07 ) . ^^^ Survival analysis in BRCA 2 carriers showed RAD 51 135C increased risk of breast and / or ovarian cancer with an HR of 4 . 0 [ 95 % confidence interval 1 . 6 9 . 8 , P = 0 . 003 ] . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Recent progress provides fresh insights into its role as a regulator of the Rad 51 recombination protein , underpinning a model in which BRCA 2 ' s involvement in chromosome stability and tumour suppression arises from its participation in recombinational processes essential for DNA replication . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The protein products of the hereditary breast cancer susceptibility genes , BRCA 1 and BRCA 2 , interact with the Rad 51 protein , a central component of homologous repair pathways . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In addition , BRCA 2 mediated enhancement of HR depended on the interaction with Rad 51 . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RAD 51 colocalizes with both BRCA 1 and BRCA 2 , and genetic variants in RAD 51 would be candidate BRCA1 / 2 modifiers . ^^^ We have identified a RAD 51 5 ' untranslated region SNP that may be associated with an increased risk of breast cancer and a lower risk of ovarian cancer among BRCA 2 mutation carriers . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 appears to function in recombination via interactions with the major eukaryotic recombinase RAD 51 [ 1 , 2 , 3 ] . ^^^ We briefly review the context in which the two studies were carried out , we comment on the results presented , and we discuss models designed to account for the role of BRCA 2 in RAD 51 mediated repair . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Cloning and sequencing full length of canine Brca 2 and Rad 51 cDNA . ^^^ Previous studies have shown that the Brca 2 tumor suppressor protein interacts with the recombinational repair protein Rad 51 . ^^^ We cloned the full length cDNA of the canine homologues of Brca 2 and Rad 51 to obtain a basis for studying their relationship with susceptibility to mammary tumors . ^^^ The canine Brca 2 and Rad 51 cDNAs are 11 and 1 . 5 kb long , encoding 3 . 471 and 339 amino acids , respectively . ^^^ Expression of both Brca 2 and Rad 51 was detected in the mammary gland , suggesting that these two genes interact in the canine mammary gland . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| A mechanistic explanation for the genome instability phenotype of Brca 2 deficient cells is provided by the observation that the nuclear localization of the central DNA repair protein in homologous recombination , Rad 51 , is reduced in 5 C 8 cells . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RESULTS : BRCA 1 and BRCA 2 are likely to act as tumor suppressor genes , and together with RAD 51 operate in a common DNA damage response pathway implicated in double strand repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In contrast , although the eight BRC repeats of mammalian BRCA 2 are believed to play an important role in HR by interacting with Rad 51 , the BRC 3 , BRC 5 , and BRC 6 repeats exhibit virtually no similarity to their mammalian counterparts . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In contrast , the role of BRCA 2 in DNA repair may be more defined by its direct interaction with the RAD 51 recombinase . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| As RAD 51 is implicated in BRCA 1 and BRCA 2 mediated tumor suppression as well as cellular viability , we have begun a functional analysis of a defined RAD 51 mutation in mammalian cells . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 protein directly associates with Rad 51 , that is essential for repair of double strand breaks ( DSBs ) by homologous recombination ( HR ) . ^^^ In this report , we study the BRCA 2 defective Chinese hamster cell mutant 5 C 8 for its ability to perform homology directed repair ( HDR ) between repeated sequences . 5 C 8 cells were recently shown to be defective in Rad 51 foci formation in response to DNA damage . ^^^ Furthermore , molecular characterization of the HDR products shows that loss of BRCA 2 in 5 C 8 cells leads to significant reduction in Rad 51 dependent gene conversion but strong enhancement of Rad 51 independent single strand annealing ( SSA ) events frequency . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The breast cancer susceptibility protein BRCA 2 controls the function of RAD 51 , a recombinase enzyme , in pathways for DNA repair by homologous recombination . ^^^ We report here the structure of a complex between an evolutionarily conserved sequence in BRCA 2 ( the BRC repeat ) and the RecA homology domain of RAD 51 . ^^^ The BRC repeat mimics a motif in RAD 51 that serves as an interface for oligomerization between individual RAD 51 monomers , thus enabling BRCA 2 to control the assembly of the RAD 51 nucleoprotein filament , which is essential for strand pairing reactions during DNA recombination . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Molecular mimicry connects BRCA 2 to Rad 51 and recombinational DNA repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| To facilitate association based linkage studies we have studied the linkage disequilibrium ( LD ) and haplotype architecture around five genes of interest for cancer risk : ATM , BRCA 1 , BRCA 2 , RAD 51 , and TP 53 . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 has a more specific role in DNA repair , regulating the activity of RAD 51 , which is required for homologous recombination . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Because the Fanconi anemia alleles of BRCA 2 fail to bind to Rad 51 in response to genotoxic stress and Rad 51 therefore fails to localize to nuclear damage foci , many investigators in the field suspect that the Fanconi anemia pathway supports the integrity of the Rad 51 and BRCA 1 and BRCA 2 pathways as they function in homologous recombination repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Thus , BRCA 2 is essential for efficient homology directed repair , presumably in conjunction with the Rad 51 recombinase . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 dependent and independent formation of RAD 51 nuclear foci . ^^^ The RAD 51 recombinase interacts directly with the breast cancer associated tumour suppressor BRCA 2 , an interaction that is required for normal recombination proficiency , radiation resistance and genome stability . ^^^ In CAPAN 1 cells , which express a truncated form of BRCA 2 that is cytoplasmic because of loss of the nuclear localization signal , the formation of IR induced RAD 51 foci is impaired . ^^^ In this work , we show that S phase RAD 51 foci form normally in CAPAN 1 cells expressing truncated BRCA 2 . ^^^ Moreover , we find that RAD 51 specifically associates with chromatin at S phase in a reaction that is BRCA 2 independent . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| It interacts directly or indirectly with a variety of important proteins , including oncogene proteins ( c myc , E2F ) , tumor suppressor proteins ( p 53 , RB , BRCA 2 ) , DNA damage repair proteins ( RAD 50 , RAD 51 ) , cell cycle regulators ( cyclin , CDK ) , transcriptional regulators ( RNA polymerase 2 ) and others related to the important biological events . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The breast cancer suppressor proteins BRCA 1 and BRCA 2 interact with RAD 51 , a protein essential for maintaining genomic stability by playing a central role in homology dependent recombinational repair of the DNA double strand breaks . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Two recent structures of BRCA 2 domains have revealed that it may serve as a critical mediator of DNA repair through direct interactions with Rad 51 , the eukaryotic homolog of RecA , and with single stranded DNA . ^^^ Before the structures were determined , little was known about the structural basis of BRCA 2 interactions with the Rad 51 pathway of DNA repair . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The product of the RAD 51 gene functions with BRCA 1 and BRCA 2 in the repair of double stranded DNA breaks . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Evidence has been accumulated to support the concept that BRCA 2 has a critical role in homologous recombination of DNA double stranded breaks by interacting with RAD 51 . ^^^ Furthermore , both the hyperphosphorylated and hypophosphorylated forms of BRCA 2 bind to RAD 51 , whereas the M phase hyperphosphorylated form of BRCA 2 no longer associates with the P / CAF , suggesting that the dissociation of P / CAF BRCA 2 complex is regulated by phosphorylation . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Full length archaeal Rad 51 structure and mutants : mechanisms for RAD 51 assembly and control by BRCA 2 . ^^^ Structural and mutational results characterize RAD 51 interactions with the breast cancer susceptibility protein BRCA 2 in higher eukaryotes . ^^^ A designed P . furiosus RAD 51 mutant binds BRC repeats and forms BRCA 2 dependent nuclear foci in human cells in response to gamma irradiation induced DNA damage , similar to human RAD 51 . ^^^ These results show that BRCA 2 repeats mimic the RAD 51 PM and imply analogous RAD 51 interactions with RAD 52 and RAD 54 . ^^^ Both BRCA 2 and RAD 54 may act as antagonists and chaperones for RAD 51 filament assembly by coupling RAD 51 interface exchanges with DNA binding . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Sequence fingerprints in BRCA 2 and RAD 51 : implications for DNA repair and cancer . ^^^ In humans , the interactions between the breast cancer susceptibility protein , BRCA 2 , and the RAD 51 recombinase are essential for DNA repair by homologous recombination ( HR ) , failure of which can predispose to cancer . ^^^ The interactions occur through conserved BRC repeat motifs , encoded in BRCA 2 , binding directly to RAD 51 . ^^^ Conservation of the BRC repeat and RAD 51 sequence fingerprints across a wide range of eukaryotic species substantiates the functional significance of the BRCA 2 RAD51 interactions . ^^^ The idea of multiple BRC repeats with binding specificity towards RAD 51 leads us to suggest a possible model for the participation of BRCA 2 in RAD 51 nucleoprotein filament formation . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Dynamic control of Rad 51 recombinase by self association and interaction with BRCA 2 . ^^^ Here , we visualize GFP Rad 51 fusion proteins in the nucleus of living cells to demonstrate the dynamic compartmentalization of Rad 51 by self association or by binding to BRCA 2 . ^^^ Mutants of Rad 51 that fail to oligomerize and / or to bind BRCA 2 distinguish three fractions of Rad 51 within the nucleoplasm : a relatively mobile fraction , an immobile oligomerized fraction , and an immobile BRCA 2 bound fraction . ^^^ This effect is specific to Rad 51 mutants that retain the capacity to bind BRCA 2 , indicating that the BRCA 2 bound fraction is selectively mobilized . ^^^ We propose that arrested replication triggers a switch between dual functions of BRCA 2 in sequestering or mobilizing a small fraction of nucleoplasmic Rad 51 and suggest a mechanism for the dynamic control of protein complexes that participate in homologous recombination . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Here , we describe the recent progress in our understanding of how Rad 51 is involved in the signalling and repair of DNA damage and how tumour suppressors , such as p 53 , ATM , BRCA 1 , BRCA 2 , BLM and FANCD 2 are linked to Rad 51 dependent pathways . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| We have isolated a holoenzyme complex termed BRCC containing BRCA 1 , BRCA 2 , and RAD 51 . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 RAD 51 DSS1 interplay examined from a microbial perspective . ^^^ The tumor suppressor BRCA 2 plays an essential role in the repair of double strand DNA breaks by regulating the action of the RAD 51 recombinase . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In a yeast two hybrid assay , the Arabidopsis Brca 2 proteins interact with Rad 51 and Dmc 1 . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| With BRCA 2 and RAD 51 , this complex is thought to have a role in the repair of DNA double strand breaks . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| A single nucleotide polymorphism in the RAD 51 gene modifies breast cancer risk in BRCA 2 carriers , but not in BRCA 1 carriers or noncarriers . ^^^ The RAD 51 gene , which participates in homologous recombination double strand breaks ( DSB ) repair in the same pathway as the BRCA 1 and BRCA 2 gene products , is a candidate for such an effect . ^^^ A single nucleotide polymorphism ( SNP ) , RAD 51 135g > c , in the 5 ' untranslated region of the gene has been found to elevate breast cancer ( BC ) risk among BRCA 2 carriers . ^^^ The estimated BC hazard ratio ( HR ) for RAD 51 135c carriers adjusted for origin ( Ashkenazi vs non Ashkenazi ) was 1 . 28 ( 95 % CI 0 . 85 1 . 90 , P=0 . 23 ) for BRCA1 / 2 carriers , and 2 . 09 ( 95 % CI 1 . 04 4 . 18 , P=0 . 04 ) when the analysis was restricted to BRCA 2 carriers . ^^^ These results indicate significantly elevated risk for BC in carriers of BRCA 2 mutations who also carry a RAD 51 135c allele . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 1 , BRCA 2 , ER alpha , ER beta , p 53 , p 21 ( waf1 / cip1 ) , CBP / P300 , RAD 51 , pS 2 and Ki 67 mRNA were quantified using real time quantitative RT PCR with an ABI 7700 apparatus . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| For example , conventional deletion of ATR , Chk 1 , Mad 2 , NBS , Rad 50 , BRCA 1 , BRCA 2 , or Rad 51 leads to developmental arrest prior to gastrulation . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Here , we review current knowledge of the BRCA 2 protein , focussing on recent studies that provide mechanistic insight into its biological function in regulating DNA recombination reactions mediated by the RAD 51 recombinase . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In particular , BRCA 1 functions in concert with Rad 51 , BRCA 2 and other genes to control double strand break repair ( DSBR ) and homologous recombination . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 is a breast cancer susceptibility gene implicated in the repair of double strand breaks by homologous recombination with RAD 51 . ^^^ We show that like BRCA 2 , DSS 1 is required for DNA damage induced RAD 51 focus formation and for the maintenance of genomic stability , indicating a function conserved from lower eukaryotes to humans . ^^^ However , DSS 1 seems to be not required for BRCA 2 or RAD 51 stability or for BRCA 2 and RAD 51 to interact , raising the possibility that DSS 1 may be required for the BRCA 2 RAD51 complex to become associated with sites of DNA damage . . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| We observed an inverse relationship between HDR and SSA when RAD 51 or BRCA 2 was impaired , i . e . , HDR was reduced but SSA was increased . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Similar effects of Brca 2 truncation and Rad 51 paralog deficiency on immunoglobulin 5 gene diversification in DT 40 cells support an early role for Rad 51 paralogs in homologous recombination . ^^^ Although recent evidence supports at least Xrcc 3 and Rad51C playing a role late in HR , our data suggest that Brca 2 and the Rad 51 paralogs may also contribute to HR at the same early step , with their loss resulting in the stimulation of an alternative , error prone repair pathway . . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Fanconi anemia complementation group D 2 ( FANCD 2 ) functions independently of BRCA 2 and RAD 51 associated homologous recombination in response to DNA damage . ^^^ The BRCA 2 breast cancer tumor suppressor is involved in the repair of double strand breaks and broken replication forks by homologous recombination through its interaction with DNA repair protein Rad 51 . ^^^ These observations suggest that the FA pathway and the BRCA 2 and Rad 51 repair pathway may be linked , although a functional connection between these pathways in DNA damage signaling remains to be determined . ^^^ We show that in response to DNA damage , BRCA 2 dependent Rad 51 nuclear focus formation was normal in the absence of FANCD 2 and that FANCD 2 nuclear focus formation and mono ubiquitination appeared normal in BRCA 2 deficient cells . ^^^ Furthermore , we established that depletion of BRCA 2 or Rad 51 had a greater effect on cell survival in response to MMC than depletion of FANCD 2 and that depletion of BRCA 2 in FANCD 2 mutant cells further sensitized these cells to MMC . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 homologue Brh 2 nucleates RAD 51 filament formation at a dsDNA ssDNA junction . ^^^ The 3 , 418 amino acid BRCA 2 contains eight approximately 30 amino acid BRC repeats that bind RAD 51 ( refs 5 , 6 ) and a approximately 700 amino acid DBD domain that binds ssDNA . ^^^ Here we show that a full length BRCA 2 homologue ( Brh 2 ) stimulates Rad 51 mediated recombination at substoichiometric concentrations relative to Rad 51 . ^^^ These results establish a BRCA 2 function in RAD 51 mediated DSB repair and explain the loss of this repair capacity in BRCA 2 associated cancers . . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 interacting protein BCCIP functions in RAD 51 and BRCA 2 focus formation and homologous recombinational repair . ^^^ RAD 51 and BRCA 2 colocalization in nuclear foci is a hallmark of HRR . ^^^ BRCA 2 has important roles in RAD 51 focus formation and HRR of DNA double strand breaks ( DSBs ) . ^^^ We further show that chromatin bound BRCA 2 colocalizes with BCCIP nuclear foci and that most radiation induced RAD 51 foci colocalize with BCCIP . ^^^ Reducing BCCIPalpha by 90 % or BCCIPbeta by 50 % by RNA interference markedly reduces RAD 51 and BRCA 2 foci and reduces HRR of DSBs by 20 to 100 fold . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The mutational status of the BRCA 2 gene in exon 11 , the largest exon harboring the RAD 51 interacting BRC domains which are critical for BRCA 2 function , was screened by polymerase chain reaction / single strand conformation polymorphism ( PCR SSCP ) analysis followed by direct sequencing . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Brh 2 , the BRCA 2 homolog in Ustilago maydis , functions in recombinational repair of DNA damage by regulating Rad 51 and is , in turn , regulated by Dss 1 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RAD 51 dependent and independent roles of a Caenorhabditis elegans BRCA 2 related protein during DNA double strand break repair . ^^^ The BRCA 2 tumor suppressor is implicated in DNA double strand break ( DSB ) repair by homologous recombination ( HR ) , where it regulates the RAD 51 recombinase . ^^^ We describe a BRCA 2 related protein of Caenorhabditis elegans ( CeBRC 2 ) that interacts directly with RAD 51 via a single BRC motif and that binds preferentially to single stranded DNA through an oligonucleotide oligosaccharide binding fold . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Other groups have also shown how BRCA 2 , RAD 51 and DSS 1 co exist in a mnage trois and how the disruption of any one of the three cohabitants can have disastrous consequences for the cell . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 has been implicated in the maintenance of genome stability and RAD 51 mediated homologous recombination repair of chromosomal double strand breaks ( DSBs ) , but its role in these processes is unclear . ^^^ At direct repeats , BRCA 2 suppresses both spontaneous gene conversion and deletions , which can arise either from crossover or RAD 51 independent sister chromatid replication slippage ( SCRS ) , but following chromosomal DSBs , BRCA 2 highly promotes gene conversion with little effect on deletions . ^^^ From these results , we conclude that ( 1 ) BRCA 2 regulates RAD 51 recombination in response to the type of DNA damage and ( 2 ) BRCA 2 suppresses SCRS , suggesting a role for BRCA 2 in sister chromatids cohesion and / or alignment . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Accessory proteins for this reaction include the Rad 51 paralogs and BRCA 2 . ^^^ Furthermore , radiation induced foci formation of Rad 51 and Rad 54 is impaired in all Rad 51 paralog and BRCA 2 mutant cell lines tested , while Rad 52 foci formation is not influenced by a mutation in any of these recombination proteins . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Screening for RAD 51 and BRCA 2 BRC repeat mutations in breast and ovarian cancer families . ^^^ In response to double strand DNA damage the co operation between RAD 51 and BRCA 2 is of great importance , and the conserved BRC repeat motifs in BRCA 2 are crucial for this interaction . ^^^ In the current study , patients belonging to 126 breast and / or ovarian cancer families were screened for RAD 51 and BRCA 2 BRC repeat mutations in order to uncover aberrations that may contribute to hereditary cancer susceptibility . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In this study , we found a novel insertion / deletion polymorphism ( 10204insAAA causing amino acid change M3332IK ) in canine BRCA 2 , which is located in the putative second nuclear localization signal ( NLS 2 ) and C terminal Rad 51 binding region . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Rad 51 , Rad 52 , Rad 54 , BRCA 1 , BRCA 2 ) in both normal and malignant cultures . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The molecular perspective : RAD 51 and BRCA 2 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| On the other hand , we did not find any difference during gametogenesis in mice harboring exon 27 truncating mutation of the Brca 2 gene and control mice , and in both cases , Rad 51 localized to the recombination foci . ^^^ Our results suggest that exon 27 of murine Brca 2 is crucial for the interaction of Brca 2 and Fancd 2 in Rad 51 mediated recombination in response to DNA damage , but that this interaction is not taking place in the homologous recombination during meiosis . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Although FA B patient HSC 230 was previously reported to also have biallelic BRCA 2 mutations , we found normal Rad 51 foci formation in cells from this patient , consistent with the recent identification of an 10 linked gene being mutated in four unrelated FA B patients . ^^^ Thus , our data show that none of the FA proteins , except BRCA 2 , are required to sequester Rad 51 into nuclear foci . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The molecular perspective : RAD 51 and BRCA 2 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| We found absence of nuclear expression and presence of cytoplasmic expression of RAD 51 in BRCA 2 tumors that differentiate them from other familial tumors . ^^^ Because BRCA 2 tumors present a specific immunohistochemical profile for RAD 51 and CHEK 2 markers that is different from non BRCA1 / 2 tumors , we have built a multivariate model with these markers that distinguish both tumors with an estimated probability of at least 76 % . ^^^ CONCLUSION : Our results suggest that BRCA 2 tumors demonstrate more cytoplasmic and less nuclear RAD 51 staining , and increased CHEK 2 staining . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The different genes involved in the BRCA 1 and BRCA 2 pathways ( GADD45A , BARD 1 , JUN , BAX , RB 1 , ERalpha , ERbeta , BAP 1 , TNFalpha , p 53 , p21Waf1 / Cip1 , p 300 , RAD 51 , pS 2 , Ki 67 ) were quantified by real time quantitative RT PCR , using the TaqMan method and an ABI Prism 7700 Sequence Detector ( Applied Biosystems ) . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Because a similar interface is involved in the binding of human Rad 51 to the conserved BRC repeat of BRCA 2 protein , the RecB domain may be one of several structural domains that interact with and recruit DNA strand exchange proteins to DNA . . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| XRCC 3 is one out of five RAD 51 paralogues and is involved in homologous recombination , as are the BRCA 1 and BRCA 2 proteins . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Recombination mediator and Rad 51 targeting activities of a human BRCA 2 polypeptide . ^^^ The results thus clarify the role of BRCA 2 in Rad 51 dependent DNA recombination and repair , and the experimental strategies described herein should be valuable for systematically deciphering this BRCA 2 function . . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The breast cancer susceptibility protein BRCA 2 is implicated in the DNA double strand break ( DSB ) repair pathway through its association with Rad 51 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| In contrast , repair factors ( Rad 51 , Rad 52 , BRCA 2 , and FANCD 2 ) , ATM and Rad 3 related ( ATR ) cascade ( ATR , ATR interacting protein , and replication protein A ) , and the DNA clamp ( Rad 17 and 9 ) accumulate in subchromatin microcompartments delineated by single stranded DNA ( ssDNA ) . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA2 / FANCD1 protein is connected to the FA pathway by interactions with the FANCG and FANCD 2 proteins , both of which co localise with the RAD 51 recombinase , which is regulated by BRCA 2 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The results showed association of Rad 51 , Rad 54 , BRCA 1 and BRCA 2 , but not Rad51C , with the nuclear matrix fraction in response to double strand breaks induction . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| We analyzed 49 SNPs in BRCA 1 , BRCA 2 , ESR 1 , XRCC 1 , XRCC 2 , XRCC 3 , NBN , RAD 51 , RAD 52 , LIG 4 , ATM , BCL 2 , TGFB 1 , MSH 6 , ERCC 2 , XPF , NR3C1 , CYP1A1 , CYP2C9 , CYP2C19 , CYP3A5 , CYP2D6 , CYP11B2 , and CYP17A1 genes using the Pyrosequencing technique . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The previously described Chinese hamster cell mutant 5 C 8 that is defective in Brca 2 shows a very complex phenotype , including increased sensitivity towards a wide variety of DNA damaging agents , chromosomal instability , abnormal centrosomes and impaired formation of Rad 51 foci in response to DNA damage . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| FA proteins interact with various proteins involved in DNA damage response and cell cycle checkpoint regulation , such as : RAD 51 , BRCA 1 , BRCA 2 , ATM or NBS 1 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Although BRCA 2 is known to play a pivotal role in homologous recombination repair by regulating Rad 51 recombinase , the precise functional relationship between BRCA 2 and the other FA genes is unclear . ^^^ Here we show that BRCA 2 dependent chromatin loading of Rad 51 after mitomycin C treatment was not compromised by disruption of FANCC or FANCD 2 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The high level of spontaneous chromosomal aberrations in Brca 2 mutant cells was largely suppressed by the BRC RPA fusion proteins , supporting the notion that the primary role of BRCA 2 in maintaining genomic integrity is in HDR , specifically to deliver Rad 51 to ssDNA . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| RAD 51 interacts directly or indirectly with a number of proteins implicated in breast cancer , such as BRCA 1 and BRCA 2 . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| Monoubiquitinated FANCD 2 translocates to damage induced nuclear foci containing BRCA 1 , BRCA 2 , and Rad 51 , thereby protecting the genome . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 tumour suppressor regulates the RAD 51 recombinase during double strand break ( DSB ) repair by homologous recombination ( HR ) but how BRCA 2 executes its functions is not well understood . ^^^ We previously described a functional homologue of BRCA 2 in Caenorhabditis elegans ( CeBRC 2 ) that binds preferentially to single stranded DNA via an OB fold domain and associates directly with RAD 51 via a single BRC domain . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| A region of human BRCA 2 containing multiple BRC repeats promotes RAD 51 mediated strand exchange . ^^^ Human BRCA 2 , a breast and ovarian cancer suppressor , binds to the DNA recombinase RAD 51 through eight conserved BRC repeats , motifs of approximately 30 residues , dispersed across a large region of the protein . ^^^ BRCA 2 is essential for homologous recombination in vivo , but isolated BRC repeat peptides can prevent the assembly of RAD 51 into active nucleoprotein filaments in vitro , suggesting a model in which BRCA 2 sequesters RAD 51 in undamaged cells , and promotes recombinase function after DNA damage . ^^^ BRCA 2 ( BRC 1 8 ) binds RAD 51 nucleoprotein filaments in a ternary complex , indicating it may organize RAD 51 on DNA . ^^^ In an ionic milieu more typical of the mammalian nucleus , BRCA 2 ( BRCI 8 ) stimulates RAD 51 mediated strand exchange , suggesting it may be an essential co factor in vivo . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| BRCA 2 tumors present a phenotype opposite to BRCA 1 tumors but very similar to sporadic tumors , except that BRCA 2 overexpress some DNA repair markers such as CHEK 2 , show high cytoplasmic expression of RAD 51 , and are negative for HER 2 amplification and expression . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| The main role of BRCA 2 appears to involve regulating the function of RAD 51 in the repair by homologous recombination . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| FA D 1 cells show the same attenuation in RAD 51 foci formation , suggesting that the unknown FANCD 1 protein is similarly involved in RAD 51 foci formation , either independently or as a subsequent step in the FANCD 2 pathway . ^^^ |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| This indicates that the FANCD 1 gene product is uniquely involved in the assembly and / or stabilization of the Rad 51 complex . ^^^ Since DNA damage induced Rad 51 nuclear foci are thought to reflect repair of DNA double strand breaks by homologous recombination , our results suggest that FANCD 1 is likely to be involved in homologous recombination dependent repair . . ^^^ |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51587 and Q06609 |
Pubmed |
SVM Score :0.0 |
| NA |
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