Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
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Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
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Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.86363515
Co immunoprecipitation revealed that purified RPA interacts physically with HsRad 51 , as well as with HsDmc 1 , the homolog that is expressed specifically in meiosis . 0.86363515^^^ The interactions of human RPA with Rad 51 , replication proteins and DNA are suited to the linking of recombination to replication . . 0.60241947^^^ Interaction of human rad 51 recombination protein with single stranded DNA binding protein , RPA . 0.5219591^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We therefore propose that Rad 52 mediates the RAD 51 independent recombination through an ssDNA annealing , assisted by RPA . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Reconstitution of the strand invasion step of double strand break repair using human Rad 51 Rad52 and RPA proteins . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Basis for avid homologous DNA strand exchange by human Rad 51 and RPA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In vitro , both yeast and human Rad 52 proteins play auxiliary roles with RPA in the action of Rad 51 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Sung , Basis for avid homologous DNA strand exchange by human Rad 51 and RPA , J . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
A postsynaptic role for single stranded DNA binding protein in recA protein promoted DNA strand exchange . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Effects of overproduction of single stranded DNA binding protein on RecA protein dependent processes in Escherichia coli . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
DNA strand exchange promoted by recA protein and single stranded DNA binding protein of Escherichia coli . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Influence of single stranded DNA binding protein on recA induction in Escherichia coli . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Interactions involving RecO and RecR overcome the inhibition of RecA by single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Here , the effects of replication protein A ( RPA ) on presynaptic complex formation and DNA strand exchange activities of Rad 51 protein were examined . ^^^ With phiX 174 ssDNA , the ATPase activity of Rad 51 protein is stimulated approximately 1 . 4 fold by RPA , provided that Rad 51 protein is in excess of the ssDNA concentration ; otherwise , RPA inhibits ATPase activity . ^^^ In contrast , with ssDNA devoid of secondary structure ( poly ( dT ) , poly ( dA ) , poly ( dI ) , and etheno M 13 DNA ) , RPA does not stimulate the already elevated ATPase activity of Rad 51 protein , but inhibits activity at low Rad 51 protein concentrations . ^^^ These results suggest that Rad 51 protein and RPA exclude one another from ssDNA by competing for the same binding sites and that RPA exerts its effect on presynaptic complex formation by eliminating secondary structure to which Rad 51 protein is bound nonproductively . ^^^ DNA strand exchange catalyzed by Rad 51 protein is also greatly stimulated by RPA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 51 protein catalyzes the DNA strand exchange reaction with a dependence on ATP and on the heterotrimeric single strand DNA ( ssDNA ) binding factor replication protein A ( RPA ) . ^^^ In strand exchange , the reaction proceeds efficiently if RPA is incorporated after nucleation of Rad 51 onto ssDNA , but if RPA is present during the nucleation phase , as is likely the case in vivo , the amount of strand exchange products becomes relatively insignificant . ^^^ Inclusion of the Rad 55 Rad57 heterodimer with Rad 51 and RPA results in a marked stimulation of strand exchange , providing evidence for a role of the Rad 55 Rad57 heterodimer in overcoming the inhibitory effect of RPA . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Efficient strand exchange is contingent upon the addition of the heterotrimeric single stranded DNA binding factor replication protein A ( RPA ) after Rad 51 has nucleated onto the single stranded DNA . ^^^ However , if the single stranded DNA is incubated with Rad 51 and RPA simultaneously to mimic what may be expected to occur in vivo , the efficiency of strand exchange decreases dramatically , revealing an inhibitory effect of RPA that is distinct from its stimulatory function . ^^^ Thus , Rad 52 functions as a co factor for the Rad 51 recombinase , acting specifically to overcome the apparent competition by RPA for binding to single stranded DNA . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Several of the proteins examined have been implicated in repair / recombination and include RAD 51 , a mammalian homolog of the Escherichia coli RecA protein ; Replication Protein A ( RPA ) , a single strand DNA binding protein ; and MLH 1 , a mismatch repair protein which is a homolog of the E . coli MutL protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Previous studies have indicated that Rad 51 protein , together with the single stranded DNA binding factor replication protein A ( RPA ) , can promote the formation of heteroduplex DNA , which is a key intermediate in homologous recombination . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Immunostaining shows extensive colocalization of Rad 52 and RPA and more limited colocalization of Rad 52 with the strand exchange protein Rad 51 . ^^^ Rad 52 and RPA foci are distinct from those formed by Rad 51 , and its meiosis specific relative Dmc 1 , in that they are also detected in meiosis during replication . ^^^ Double strand breaks ( DSBs ) promote formation of RPA , Rad 52 , and Rad 51 foci . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Accompanying cytological studies with fluorescent antibodies indicated that while there was no detectable effect on the number of recombination nodules ( MLH 1 foci ) , there were marked changes in the stage of appearance and localization of RAD 51 and RPA proteins . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We have also found that the preference of strand exchange in the 5 ' to 3 ' direction does not change if RPA is replaced by Escherichia coli SSB or T 4 gene 32 proteins , suggesting that the preference for the direction of strand exchange is intrinsic to Rad 51 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
While the absence of SCP 3 affected the nuclear distribution of DNA repair and recombination proteins ( Rad 51 and RPA ) , as well as synaptonemal complex protein 1 ( SCP 1 ) , a residual chromatin organization remained in the mutant meiotic cells . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 51 catalyzed DNA strand exchange is greatly enhanced by the single stranded ( ss ) DNA binding factor RPA if the latter is introduced after Rad 51 has already nucleated onto the initiating ssDNA substrate . ^^^ Paradoxically , co addition of RPA with Rad 51 to the ssDNA to mimic the in vivo situation diminishes the level of strand exchange , revealing competition between RPA and Rad 51 for binding sites on ssDNA . ^^^ Rad 52 promotes strand exchange but only when there is a need for Rad 51 to compete with RPA for loading onto ssDNA . ^^^ The Rad 51 Rad52 complex efficiently utilizes a ssDNA template saturated with RPA for homologous pairing but does not appear to be more active than Rad 51 when an RPA free ssDNA template is used . ^^^ Rad 52 does not substitute for RPA in the pairing and strand exchange reaction nor does it lower the dependence of the reaction on Rad 51 or RPA . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Werner helicase relocates into nuclear foci in response to DNA damaging agents and co localizes with RPA and Rad 51 . ^^^ These WRNp foci overlapped with the foci of replication protein A ( RPA ) almost entirely and with the foci of Rad 51 partially , implicating cooperative functions of these proteins in response to DNA damage . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Immuno double staining experiments in Saccharomyces cerevisiae suggest that Rad 51 , the eukaryotic recombinase , can assemble at or near sites containing ssb ( replication protein A , RPA ) during the response to DNA damage , consistent with a need for mediator activity . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We show here that Rad 22 interacts with Rhp 51 and Rpa 70 ( the fission yeast homologues of Rad 51 and the large subunit of RPA , respectively ) , but that neither of these proteins appears to be responsible for the 83 kDa species . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We also examined the functional interaction of Rad51B Rad51C with Rad 51 and RPA . ^^^ Even though RPA enhances Rad 51 catalyzed DNA joint formation via removal of secondary structure in the ssDNA substrate , it can also compete with Rad 51 for binding to the substrate , leading to suppressed reaction efficiency . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Yeast Rad 51 promotes homologous pairing and strand exchange in vitro , but this activity is inefficient in the absence of the accessory proteins , RPA , Rad 52 , Rad 54 and the Rad 55 Rad57 heterodimer . ^^^ The Rad 51 I345T mutant protein shows increased binding to single and double stranded DNA , and is proficient in displacement of replication protein A ( RPA ) from single stranded DNA , suggesting that the normal function of Rad 55 Rad57 is promotion and stabilization of Rad 51 ssDNA complexes . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In vitro studies strongly suggest that several proteins of this pathway Rad 51 , Rad 52 , Rad 54 , Rad 55 , Rad 57 , and replication protein A ( RPA ) play a role in the strand exchange reaction . ^^^ However , the 5 ' ends of the broken fragments undergo extensive resection , similar to what is observed in rad 51 , rad 52 , rad 55 , and rad 57 mutants , indicating that these RPA mutants are defective in the repair of the Spo 11 dependent DSBs that initiate homologous recombination during meiosis . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 52 protein associates with replication protein A ( RPA ) single stranded DNA to accelerate Rad 51 mediated displacement of RPA and presynaptic complex formation . ^^^ This activity is stimulated by replication protein A ( RPA ) , but only when RPA is introduced after Rad 51 nucleoprotein filament formation . ^^^ In contrast , RPA inhibits Rad 51 nucleoprotein complex formation by prior binding to single stranded DNA ( ssDNA ) , but Rad 52 protein alleviates this inhibition . ^^^ Here we show that Rad 51 filament formation is simultaneous with displacement of RPA from ssDNA . ^^^ Rad 52 protein accelerates RPA displacement by Rad 51 protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We further demonstrate that RPA has a critical postsynaptic role in DNA strand exchange , stabilizing the DNA pairing initiated by Rad 51 protein . ^^^ Stabilization of the Rad 51 generated DNA pairing intermediates can be can occur either by binding the displaced strand with RPA or by degrading the same DNA strand using exonuclease 7 . ^^^ The optimal conditions for Rad 51 mediated DNA strand exchange used here minimize the secondary structure in single stranded DNA , minimizing the established presynaptic role of RPA in facilitating Rad 51 filament formation . ^^^ We verify that RPA has little effect on Rad 51 filament formation under these conditions , assigning the dramatic stimulation of strand exchange nevertheless afforded by RPA to its postsynaptic function of removing the displaced DNA strand from Rad 51 filaments . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae Rad 51 , Rad 54 , and replication protein A ( RPA ) proteins work in concert to make heteroduplex DNA joints during homologous recombination . ^^^ RPA is still indispensable for optimal reaction efficiency , but its role in this instance is to sequester free single stranded DNA , which otherwise inhibits Rad 51 and Rad 54 functions . ^^^ These results thus shed light on the function of Rad 54 in the Rad 51 mediated homologous DNA pairing reaction and also reveal a novel role of RPA in the presynaptic stage of this reaction . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 52 protein has two unique activities : facilitation of replication protein A ( RPA ) displacement by Rad 51 protein and annealing of RPA single stranded DNA ( ssDNA ) complexes . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The recombination deficient mutant RPA ( rfa 1 t11 ) is displaced slowly from single stranded DNA by Rad 51 protein . ^^^ RPA ( rfa 1 t11 ) stimulates DNA strand exchange , provided that the Rad 51 protein . ssDNA nucleoprotein complex is assembled prior to introduction of the mutant RPA . ^^^ However , RPA ( rfa 1 t11 ) is displaced from ssDNA by Rad 51 protein more slowly than wild type RPA and , as a consequence , Rad 51 protein mediated DNA strand exchange is inhibited when the ssDNA is in a complex with RPA ( rfa 1 t11 ) . ^^^ Rad 52 protein can stimulate displacement of RPA ( rfa 1 t11 ) from ssDNA by Rad 51 protein , but the rate of displacement remains slow compared with wild type RPA . ^^^ These in vitro results suggest that , in vivo , RPA is bound to ssDNA prior to Rad 51 protein and that RPA displacement by Rad 51 protein is a critical step in homologous recombination , which is impaired in the rfa 1 t11 mutation . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 52 forms complexes with Rad 51 , replication protein A ( RPA ) or Rad 59 and its presence is essential for the formation of Rad 51 Rad52 Rad 59 and RPA Rad 52 Rad59 complexes . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
RPA binding precedes binding of the Rad 51 recombination protein . ^^^ The extent of RPA binding is greater when Rad 51 is absent , supporting the idea that Rad 51 displaces RPA from ssDNA . ^^^ RPA plays an important role during RAD 51 mediated strand invasion of the MAT ssDNA into the donor sequence HML . ^^^ The replication proficient but recombination defective rfa 1 t11 ( K45E ) mutation in the large subunit of RPA is normal in facilitating Rad 51 filament formation on ssDNA , but is unable to achieve synapsis between MAT and HML . ^^^ Thus , RPA appears to play a role in strand invasion as well as in facilitating Rad 51 binding to ssDNA , possibly by stabilizing the displaced ssDNA . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Replication protein A ( RPA ) is displaced from single stranded DNA ( ssDNA ) by Rad 51 during the initiation of homologous recombination . ^^^ Interactions between these proteins have been reported , but the functional significance of the direct RPA Rad 51 interaction has yet to be elucidated . ^^^ We have identified and characterized the interaction between DNA binding domain A of RPA ( RPA70A ) and the N terminal domain of Rad 51 ( Rad51N ) . ^^^ NMR chemical shift mapping showed that Rad51N binds to the ssDNA binding site of RPA70A , suggesting a competitive mechanism for the displacement of RPA from ssDNA by Rad 51 . ^^^ Functional ATP hydrolysis assays were performed for wild type Rad 51 and a mutant defective in binding RPA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Herpes simplex virus type 1 ( HSV 1 ) DNA replication is associated with nuclear domains called ND 10 , which contain host recombination proteins such as RPA , RAD 51 , and NBS 1 and participate in the cell ' s response to DNA damage . ^^^ In this work , RPA , RAD 51 , and NBS 1 were observed predominantly in stage IIIb but not stage IIIa prereplicative sites , suggesting that the efficient recruitment of these recombination proteins is dependent on the presence of the viral polymerase and other replication proteins within these sites . ^^^ Finally , RPA , RAD 51 , and NBS 1 were found to be associated with UL 29 foci observed in transfected cells expressing UL 29 and the helicase primase heterotrimer and containing intact ND 10 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In this review , we discuss primarily those proteins involved in the initial stages of homologous recombination , including SPO 11 , MRE 11 , RAD 50 , NBS 1 , DMC 1 , RAD 51 , RAD 51 paralogs , RAD 52 , RPA , RAD 54 , and RAD54B . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Suppression of HR was also not seen with missense mutations in the p 53 core domain ( His 175 and His 273 ) , which retained the ability to interact with RPA , suggesting that the disruption of additional binding interactions of p 53 , for example , with Rad 51 or recombination intermediates , also impacts on HR . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
CDK 1 is required for efficient 5 ' to 3 ' resection of DSB ends and for the recruitment of both the single stranded DNA binding complex , RPA , and the Rad 51 recombination protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
When constitutively expressed in uninfected T lymphocytes , IRF 4 caused reduced expression of critical DNA repair genes , including Rad 51 , XRCC 1 , Ung 1 , RPA , and proliferative cell nuclear antigen ( PCNA ) , a transcriptional phenotype with striking similarities to the profile observed in HTLV infected T lymphocytes . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Survival after DNA damage was almost fully restored by a chimeric form of Brh 2 having a DNA binding domain from RPA 70 fused to the Brh 2 N terminal domain , but Rad 51 focus formation and mitotic recombination were elevated above wild type levels . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Unlike rad 51 mutants , Cebrc 2 mutants also accumulate RPA 1 at DSBs , and abnormal chromosome aggregates that arise during the meiotic prophase can be rescued by blocking the NHEJ pathway . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Using co immunoprecipitation assays , we showed that cellular interaction of RPA with two DSB repair factors , Rad 51 and Rad 52 , was predominantly mediated by the hyperphosphorylated species of RPA in cells after UV and camptothecin treatment . ^^^ Moreover , Rad 51 and Rad 52 displayed higher affinity for the hyperphosphorylated RPA than native RPA in an in vitro binding assay . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Rad 52 facilitates the binding of Rad 51 to replication protein A ( RPA ) coated single stranded DNA . ^^^ Using short single stranded DNA and homologous double stranded DNA , in the absence of RPA , we found that Rad 52 ( optimal at three per Rad 51 ) was still required for Rad 51 promoted heteroduplex joint formation in vitro , as assayed by the formation of D loops , suggesting another role for Rad 52 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
This work reports the relative timing and localization of five different meiotic proteins that have previously been implicated in human homologous recombination [ RAD 51 , replication protein A ( RPA ) , MSH 4 , MLH 1 and MLH 3 ] . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
A separate population of chromatin bound PCNA is already present in these template nuclei at discrete DNA damage foci , co localising with gamma H2AX , RPA and Rad 51 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
These molecules included not only those involved in cell cycle progression , but also essential molecules for DNA double strand break repair , such as Rad 51 and Rpa 1 . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The central part of the protein contains multiple copies of a motif that binds the Rad 51 recombinase ( the BRC repeat ) , and the C terminus contains domains that have structural similarity to domains in the ssDNA binding protein replication protein A ( RPA ) . ^^^ The high level of spontaneous chromosomal aberrations in Brca 2 mutant cells was largely suppressed by the BRC RPA fusion proteins , supporting the notion that the primary role of BRCA 2 in maintaining genomic integrity is in HDR , specifically to deliver Rad 51 to ssDNA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Reinvasion is favored when the displaced strand is coated with RecA whereas it is blocked when it lacks RecA , remains covered by single stranded DNA binding protein or is removed by a single strand specific exonuclease . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We find that recA 730 protein is more proficient than the wild type recA protein in the competition with single stranded DNA binding protein ( SSB protein ) for single stranded DNA ( ssDNA ) binding sites . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Conditions that removed the formation of secondary structure in the gapped DNA , such as addition of Escherichia coli single stranded DNA binding protein or preincubation in 1 mM MgCl 2 , optimized the binding of RecA protein and increased the formation of joint molecules . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The ability of T 7 gene 2 . 5 protein to stimulate the activity and processivity of T 7 DNA polymerase is compared with the ability of three other single stranded DNA binding proteins : E . coli single stranded DNA binding protein , T 4 gene 32 protein , and E . coli recA protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We describe the formation of homologously paired joint molecules in an in vitro reaction that is dependent on the concerted actions of purified RecA and RecBCD proteins and is stimulated by single stranded DNA binding protein ( SSB ) . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We find that , while the recA 430 protein possesses ssDNA dependent rATP activity , this activity is inhibited by the Escherichia coli single stranded DNA binding protein ( SSB protein ) under many conditions that enhance wild type recA protein rATPase hydrolysis . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Stabilization of recA protein ssDNA complexes by the single stranded DNA binding protein of Escherichia coli . ^^^ In vitro recombination reactions promoted by the recA protein of Escherichia coli are enhanced by the single stranded DNA binding protein ( SSB ) . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
When E . coli single stranded DNA binding protein ( SSB ) coats single stranded DNA ( ssDNA ) in the presence of 1 mM MgCl 2 it inhibits the subsequent binding of recA protein , whereas SSB binding to ssDNA in 12 mM MgCl 2 promotes the binding of recA protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
SSB protein ( where SSB is Escherichia coli single stranded DNA binding protein ) complexes are formed prior to the addition of recA protein , at low magnesium ion concentration in the presence of spermidine , and at low ATP concentrations . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The ability of TFIIIA to rapidly promote DNA renaturation is unique when compared with Escherichia coli recA protein , single stranded DNA binding protein , or bacteriophage T 4 gene 32 protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The suboptimal conditions tested included addition of single stranded DNA binding protein to single stranded DNA prior to addition of RecA . ^^^ We hypothesize that the ability of RecA 803 to overcome interference by single stranded DNA binding protein is the property that allows recA 803 to suppress partially the deficiency in repair caused by recF mutations in the uvrA 6 background . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Continuous association of Escherichia coli single stranded DNA binding protein with stable complexes of recA protein and single stranded DNA . ^^^ The single stranded DNA binding protein of Escherichia coli ( SSB ) stimulates recA protein promoted DNA strand exchange reactions by promoting and stabilizing the interaction between recA protein and single stranded DNA ( ssDNA ) . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In addition to RecA protein and RecBCD enzyme , inversion by the RecBC pathway required single stranded DNA binding protein , DNA gyrase , DNA polymerase 1 and DNA ligase . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Further examination of ATP hydrolysis ( under strand exchange conditions ) revealed that the ATPase activity of the [ Asn 160 ] recA protein is strongly suppressed in the presence of Escherichia coli single stranded DNA binding protein ( a component of the strand exchange assay ) , whereas the ATPase activity of the wild type recA protein is stimulated by the E . coli protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Reversibility of strand invasion promoted by recA protein and its inhibition by Escherichia coli single stranded DNA binding protein or phage T 4 gene 32 protein . ^^^ Escherichia coli single stranded DNA binding protein or phage T 4 gene 32 protein largely suppressed the formation of this second population of networks by inactivating the newly formed heteroduplex DNA , which , however , could be reactivated when recA protein was dissociated by incubation at 0 degrees C . ^^^ These observations indicate that the nature of the nucleoprotein products of strand exchange determines whether pairing and strand exchange are reversible or not , and they further suggest a new explanation for the way in which E . coli single stranded DNA binding protein and gene 32 protein accelerate the apparent forward rate of strand exchange promoted by recA protein , namely by suppressing initiation of the reverse reaction . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Formation of D loops during the exchange of strands between a circular single stranded DNA and a completely homologous linear duplex proceeds optimally when the duplex DNA is added to the complex of recA protein and single stranded DNA formed in the presence of single stranded DNA binding protein and ATP . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
RecA catalyzed strand separation is effective only for very short duplexes , not exceeding 30 bp , and is not stimulated by single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The topics discussed are : elementary properties of the protein ; its main functions in the cell ( recombination and SOS response ) ; the formation , dissociation and regulation of the activated RecA protein complex and its cofactors , including the single stranded DNA binding protein ( SSB ) ; functional domains in the recA gene structure ; formation of RecA protein complex with single and double stranded DNA ; RecA like proteins in different microorganisms . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
A protein promoted DNA strand exchange . recA protein and circular single stranded DNA form a stable complex in the presence of single stranded DNA binding protein ( SSB ) , in which one recA protein monomer is bound per two nucleotides of DNA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
This increase results from the formation of a relatively stable recA protein single stranded DNA complex that is similar to the complex formed in the presence of the single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
On the role of single stranded DNA binding protein in recA protein promoted DNA strand exchange . ^^^ RecA protein promoted DNA strand exchange is greatly stimulated by the single stranded DNA binding protein ( SSB ) of Escherichia coli . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The complete exchange of strands between circular single stranded and full length linear duplex DNAs promoted by the recA protein of Escherichia coli is dependent upon the hydrolysis of ATP and is strongly stimulated by the single stranded DNA binding protein ( SSB ) . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli . recA protein , in the presence of single stranded DNA binding protein and ATP , promotes the complete exchange of strands between circular single stranded DNA containing pyrimidine dimers and a homologous linear duplex , converting the pyrimidine dimer containing single stranded DNA to a circular duplex . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Complete strand exchange requires a stoichiometric amount of recA protein and is strongly stimulated by the single stranded DNA binding protein of E . coli . . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Stable complexes of recA protein and single stranded DNA formed in the presence of ATP and single stranded DNA binding protein . ^^^ In the presence of ATP and the single stranded DNA binding protein , recA protein and single stranded DNA interact to form a complex whose stability depends upon the single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In addition , we have found that using single stranded DNA binding protein ( SSB ) or recA protein in DNA sequencing reactions dramatically increases the resolution of sequencing runs . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Biochemical interaction of the Escherichia coli RecF , RecO , and RecR proteins with RecA protein and single stranded DNA binding protein . ^^^ As shown by other workers , joint molecule formation by RecA was inhibited by E . coli single stranded DNA binding protein ( SSB ) when it was added to single stranded DNA before RecA . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Several DNA binding proteins ( T 7 RNA polymerase , UvrB , single stranded DNA binding protein of Escherichia coli , T 4 gp32 , and RecA of E . coli ) are shown to cross link to single stranded psoralen monoadducted DNA oligos differing in length and sequence . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The products of the recF , recO , and recR genes are thought to interact and assist RecA in the utilization of single stranded DNA precomplexed with single stranded DNA binding protein ( Ssb ) during synapsis . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The RecOR facilitated binding of RecA protein to single stranded DNA ( ssDNA ) that is bound by single stranded DNA binding protein ( SSB ) is much faster if the ssDNA is linear , suggesting that a DNA end ( rather than a gap ) facilitates binding . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Complete inhibition of Streptococcus pneumoniae RecA protein catalyzed ATP hydrolysis by single stranded DNA binding protein ( SSB protein ) : implications for the mechanism of SSB protein stimulated DNA strand exchange . ^^^ The ATP dependent three strand exchange activity of the Streptococcus pneumoniae RecA protein ( RecA ( Sp ) ) , like that of the Escherichia coli RecA protein ( RecA ( Ec ) ) , is strongly stimulated by the single stranded DNA binding protein ( SSB ) from either E . coli ( SSB ( Ec ) ) or S . pneumoniae ( SSB ( Sp ) ) . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Here , we show that Mycobacterium tuberculosis RecX binds directly to M . tuberculosis RecA as well as M . smegmatis and E . coli RecA proteins in vivo and in vitro , but not single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
The C terminus of the Escherichia coli RecA protein modulates the DNA binding competition with single stranded DNA binding protein . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
We report the reconstitution of the initial steps of the double strand break repair pathway where joint molecule formation between a duplex DNA fragment and a circular template by the combined action of RecA , RecBCD , and the single stranded DNA binding protein provides the substrate for replication fork formation by the restart primosome and the DNA polymerase 3 holoenzyme . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In contrast to previously published data , the single stranded DNA binding protein ( SSB ) is not absolutely required for Pol 5 mediated lesion bypass provided ATP , instead of ATPgammaS , activates the RecA filament . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Already 10 ray analysed are Mycobacterium tuberculosis and Mycobacterium smegmatis RecA and their nucleotide complexes , and different crystal forms of M . tuberculosis single stranded DNA binding protein , M . smegmatis DNA binding protein from stationary phase cells and M . tuberculosis ribosome recycling factor . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
In vitro , efficient RecA filament formation onto single stranded DNA binding protein ( SSB ) coated circular single stranded DNA ( ssDNA ) in the presence of RecX occurs only when all of the RecFOR proteins are present . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
Although ordinarily single stranded DNA binding protein ( SSB ) prevents filament nucleation , single RecA monomers can easily be added to an existing filament and displace SSB from DNA at the rate of filament extension . ^^^
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06609 and P27694	Pubmed	SVM Score :0.0
NA