| Interacting proteins: Q9UN19 and Q06187 |
Pubmed |
SVM Score :0.0 |
| We have found that the PH domains of Bam 32 and tandem PH domain containing protein 2 ( TAPP 2 ) specify a temporally distinct wave of membrane recruitment compared with that of Bruton ' s tyrosine kinase ( Btk ) , with recruitment of these two adaptors representing a later stage of the response . ^^^ This differential regulation can be accounted for by Src homology 2 containing inositol polyphosphate 5 phosphatase ( SHIP ) activity alone , as expression of membrane targeted SHIP completely abrogated Btk recruitment , but had no inhibitory effect on Bam 32 or TAPP 2 recruitment . ^^^ |
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| Interacting proteins: Q9UN19 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Like Bam 32 , BCR driven TAPP 1 and TAPP 2 recruitment is a relatively slow and sustained response , in contrast to Btk recruitment and Ca ( 2+ ) mobilization responses , which are rapid and transient . ^^^ |
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| Interacting proteins: Q9UN19 and Q06187 |
Pubmed |
SVM Score :0.0 |
| In endothelial cells overexpressing wild type platelet derived growth factor beta ( PDGFbeta ) receptors , which express Bmx and Src as their major Btk ( Bruton ' s tyrosine kinase ) family and Src family tyrosine kinases , respectively , PDGF can stimulate PI3K dependent tyrosine phosphorylation of DAPP 1 . ^^^ We show that in DT 40 chicken B cell lines , antibody stimulation leads to PI3K dependent tyrosine phosphorylation of DAPP 1 that is lost in Lyn or Syk deficient cell lines but not Btk deficient cell lines . ^^^ Finally , in vitro we find lipid modified Src to be the most effective DAPP 1 tyrosine kinase ( versus Syk , Lyn , Btk , and Bmx ) ; phosphorylation of DAPP 1 but not Src autophosphorylation is stimulated approximately 10 fold by PtdIns ( 3 , 4 , 5 ) P ( 3 ) ( IC ( 50 ) = 150 nm ) and phosphatidylinositol 3 , 4 bisphosphate but not by their nonbiological diastereoisomers and depends on PH domain mediated binding of DAPP 1 to PtdIns ( 3 , 4 , 5 ) P ( 3 ) containing membranes . ^^^ |
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