| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Pleckstrin homology and Src homology 2 domains of Btk were required for PLC gamma 2 activation . ^^^ Since Syk is also required for the BCR induced PLC gamma 2 activation , our findings indicate that PLC gamma 2 activation is regulated by Btk and Syk through their concerted actions . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| We propose that btk can substitute for syk in inducing phospholipase C gamma 2 tyrosine phosphorylation and initiating calcium mobilization in CD 72 stimulated B lymphocytes . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| PLC gamma 2 activation in EMF stimulated cells is mediated by stimulation of the Bruton ' s tyrosine kinase ( BTK ) , a member of the Src related TEC family of protein tyrosine kinases , which acts downstream of LYN kinase and upstream of PLC gamma 2 . ^^^ B cells rendered BTK deficient by targeted disruption of the btk gene did not show enhanced PLC gamma 2 activation in response to EMF exposure . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Cross linking of the B cell receptor induces activation of phospholipase D through Syk , Btk and phospholipase C gamma 2 . ^^^ Herein we provide biochemical and genetic evidence that cross linking of the B cell receptor ( BCR ) induces rapid activation of PLD through a Syk , Btk and phospholipase C ( PLC ) gamma 2 dependent pathway in DT 40 cells . ^^^ These results demonstrate that Syk , Btk and PLC gamma 2 are essential for BCR induced PLD activation . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Cell lines deficient for Btk are impaired in phospholipase C gamma 2 ( PLCgamma 2 ) dependent signaling . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Phosphatidylinositol 3 , 4 , 5 trisphosphate regulates Ca ( 2+ ) entry via btk in platelets and megakaryocytes without increasing phospholipase C activity . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Here , based upon the available genetic and biochemical data of the BCR signal components , we discuss several mechanisms by which BCR signals are propagated and modified , with specific emphasis on the phospholipase C ( PLC ) gamma 2 calcium pathway Gene targeting experiments in DT 40 chicken B cells highlighted the importance of the intracellular protein tyrosine kinases Syk and Btk in PLC gamma 2 activation . ^^^ Until recently , the molecular mechanism underlying the double requirement for Syk and Btk in PLC gamma 2 activation remained unclear , but new data suggest that an adapter molecule , B cell linker protein ( alternatively named SLP 65 or BASH ) , phosphorylated by Syk , provides docking sites for Btk SH 2 domain as well as PLC gamma 2 SH2 domains , thus bringing Btk into close proximity with PLC gamma 2 . ^^^ The activated Btk then phosphorylates PLC gamma 2 , leading to its activation . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Btk has been proposed to participate in B cell antigen receptor induced signaling events leading to activation of phospholipase C gamma 2 ( PLCgamma 2 ) and calcium mobilization . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Four tyrosine residues in phospholipase C gamma 2 , identified as Btk dependent phosphorylation sites , are required for B cell antigen receptor coupled calcium signaling . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Here we show that the BCR mediated Rac 1 activation is significantly inhibited by loss of Btk , while this Rac 1 activation is not affected by loss of phospholipase C gamma 2 ( PLC gamma 2 ) . ^^^ Since PLC gamma 2 is also required for BCR mediated JNK 1 response , our results suggest that Btk regulates Rac 1 pathway as well as PLC gamma 2 pathway , both of which contribute to the BCR mediated JNK 1 response . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Cbl b positively regulates Btk mediated activation of phospholipase C gamma 2 in B cells . ^^^ Cbl b interacted with PLC gamma 2 and helped the association of PLC gamma 2 with Bruton ' s tyrosine kinase ( Btk ) , as well as B cell linker protein ( BLNK ) . ^^^ These results demonstrate that Cbl b positively regulates BCR mediated Ca2+ signaling , most likely by influencing the Btk / BLNK / PLC gamma 2 complex formation . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| BTK targets nuclear factor kappaB ( NF kappaB ) to activate the bcl 10 promoter via a phospholipase C gamma 2 ( PLC gamma 2 ) dependent mechanism . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| The Tec family kinase Btk plays an important role in the regulation of phospholipase C gamma 2 ( PLC gamma 2 ) downstream of the collagen receptor glycoprotein 6 ( GPVI ) in human platelets . ^^^ However , Btk / / Tec / platelets exhibit a greater reduction in PLC gamma 2 phosphorylation than is seen in the absence of Btk , thus revealing an important role for Tec in this situation . ^^^ These results demonstrate an essential requirement for Tec and Btk in platelet activation by GPVI and reveal a functional role for Tec in the regulation of PLC gamma 2 in the absence of Btk . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Significantly , 2 APB induced InsP ( 3 ) R and TRPC 3 activation was prevented in DT 40 knockout cells devoid of the BCR and PLC gamma 2 coupled adaptor / kinases , Syk , Lyn , Btk or BLNK . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| We discovered that Bruton ' s tyrosine kinase ( BTK ) and phospholipase C gamma 2 ( PLC gamma 2 ) are required to activate NFAT and NF kappaB , and to produce the lipid second messenger diacylglycerol in response to BCR cross linking . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| The major Btk dependent substrate in B cells , phospholipase C gamma 2 ( PLC gamma 2 ) , functions to generate the key secondary messengers , inositol 1 , 4 , 5 trisphosphate and diacylglycerol . ^^^ We hypothesized that Btk functioned independently of PLC gamma 2 in the CD 38 signaling pathway . ^^^ Taken together , these data demonstrate that CD 38 initiates a novel signaling cascade leading to Btk , PC PLC , and phospholipase D dependent , PLC gamma 2 independent , B lymphocyte activation . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Analysis of mutants of the chicken B cell line DT 40 deficient in either Syk , Lyn , Btk , or PLC gamma 2 revealed that BCR induced activation of PKC mu , like activation of PLC gamma 2 , requires Syk and is partially regulated by Btk , but is Lyn independent . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Overexpression of a catalytically inactive form of SHP 1 prevents the PIR B mediated inhibition of tyrosine phosphorylation of Syk , Btk , and PLC gamma 2 . ^^^ Dephosphorylation of Syk and Btk mediated by SHP 1 leads to a decrease of their kinase activity , which in turn inhibits tyrosine phosphorylation of PLC gamma 2 . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Null mutations in either PLCG 2 or BTK are associated with decreased numbers of mature B cells , failure to make antibodies to some T cell independent antigens and the absence of CD5+ peritoneal B cells . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Syk and Btk phosphorylate peptides from PLCg 2 containing Tyr 753 and Tyr 759 respectively , suggesting that they may stimulate phosphorylation at these sites in phospholipase . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Specific phosphoacceptor sites in SLP 65 serve as docking sites for Ca ( 2+ ) mobilizing enzymes Btk and PLC gamma 2 . ^^^ Thus , elevation of Ca ( 2+ ) is regulated by at least two signaling modules , the B cell specific Ca ( 2+ ) initiation complex comprising SLP 65 , Btk , and PLC gamma 2 and the more ubiquitously expressed NTAL / Grb2 complex , which acts as an amplifier by switching off inhibitory elements . . ^^^ |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16885 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|