Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Antibodies to PAP 3 detected PAP 3 and inositol 1 , 2 cyclic phosphate 2 phosphohydrolase with identical reactivity on immunoblotting . ^^^ Lipocortin 3 has also been previously identified as placental anticoagulant protein 3 ( PAP 3 ) and calcimedin 35 alpha . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Cytological follow up and histological results of 2336 patients with Pap 3 and 10584 with Pap 2 control of 1973 and 1974 were checked up to May 1976 and compared with new literature . 50 % of Pap 3 became to negatives , 10 % of Pap 3 and 0 , 82 % of Pap 2 control became positive ( Pap 4 and Pap 5 ) . ^^^ Up to now histological findings has been resulted in 10 % invasive cancers ; in 53 % carcinomata in situ and severe dysplasia , in 16 % moderate and mild dysplasias , and in 5 % normal epithelium from repeated Pap 3 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The isolated proteins ( PAP 1 a , PAP 1 b and PAP 2 ) were homogeneous in various methods including 7 . 5 % and 20 % gel electrophoresis or gel chromatography , and share , in the extract , 85 % of the total of the acidic proteins that migrate with the bromophenol blue marker in 7 . 5 % gels . ^^^ These two proteins were identified to be the nervous system specific protein S 100 by immunochemical and electrophoresis methods as well as by amino acid analysis , and the other protein PAP 2 was revealed to be a calcium binding protein . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The phosphohydrolase activity , which is insensitive to N ethylmaleimide ( PAP 2 ) , is specifically located in the plasma membrane ( Jamal , Z . , Martin , A . , Gomez Muoz , A . and Brindley , D . ^^^ Saturated fatty acids , albumin , sphingosine and propranolol have no significant effects on PAP 2 activity . ^^^ However , chlorpromazine decreases PAP 2 activity by about 14 % . ^^^ Linolenate , arachidonate and eicosapentaenoate at 1 mM also produced small ( 7 10 % ) decreases in PAP 2 activity . ^^^ It is proposed that both PAP 1 and PAP 2 activities may be involved in signal transduction , although the main function of PAP 1 seems to be involved in the synthesis of glycerolipids . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PAP 2 activity was insensitive to N ethylmaleimide and did not require Mg2+ for activity . ^^^ Chronic treatment with ethanol increased the activity of PAP 2 in the hearts of the corpulent males , but had no effect in the corpulent females . ^^^ The specific activity of PAP 2 was higher in subcutaneous , gonadal and peri renal adipose depots in the females and in the peri renal depot of the corpulent males compared with the lean genotypes . ^^^ The tissue specificity and the sex differences in the specific activities of PAP 1 and PAP 2 are discussed in terms of their proposed functions in glycerolipid biosynthesis and signal transduction . ^^^ It is proposed that a decreased activity of PAP 2 could be involved in the insulin insensitivity in the corpulent rats . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
To evaluate the changed policy regarding cervical smears with mild atypia in the Netherlands ( before 1988 classified as Pap 2 , thereafter as Pap IIIA ) , an descriptive study was carried out in the laboratory of gynaecological cytology Cyt U Universitair ( University of Utrecht ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The patients with PAP 2 smears had a high incidence of HPV ( 67 % ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Of the 109 smears positive for Chlamydia trachomatis , 90 showed cervical cells with reactive changes ( out of 1 , 490 smears with PAP 2 ) , whereas no cytological changes were found in 15 cases ( out of 884 smears with PAP 1 ) . ^^^ Changes suggestive of mild or moderate dysplasia were found in only four cases ( out of 93 smears with PAP 3 ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
From the cytologically normal smears ( Pap 1 , 2 , IIw ) 57 / 424 ( 13 % ) 16 / 18 were found HPV positive and from the pathological ones ( Pap 3 , IIID , IVa , IVb , 5 ) , 30 / 92 ( 33 % ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
These include the monomeric enzymes PAP , PAP S , PAP 2 , and dodecandrin and the enzymatic A chains from the heterodimeric toxins ricin and modeccin . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
In 8 . 3 % of the cases the results were obtained through PAP 3 according to the scheme of Papanicolaou . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The pokeweed antiviral protein , previously identified in two forms ( PAP and PAP 2 ) in the leaves of Phytolacca americana ( pokeweed ) [ Obrig . ^^^ PAP and PAP 2 . 3 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
This comparison showed an accuracy of 64 . 5 % and if the PAP 3 findings were included , of 76 . 6 % in diagnosing prostatic carcinoma by the cytological method . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
BACKGROUND : The pancreatitis associated protein ( PAP ) 1 and 3 , but not PAP 2 , mRNAs are constitutively expressed in the small intestine of rats . ^^^ METHODS : We studied expression of both PAP 1 and PAP 3 mRNAs during development and on nutritional and hormonal manipulations . ^^^ Finally , whereas thyroidectomy and ovariectomy induced a decrease in both mRNA concentrations , and adrenalectomy a limited decrease in PAP 3 mRNA only , diabetes and castration did not alter the expression of either gene . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
We have previously reported the coding sequence of the rat PAP 2 mRNA . ^^^ We show in this paper the existence in rat pancreas of two forms of PAP 2 mRNA with identical coding sequence but a different 5 ' untranslated region . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NE PAP 1 had chromatographic properties similar to those of S 100 PAP , and both enzymes displayed higher activities in the presence of Mn2+ than in the presence of Mg2+ , whereas NE PAP 2 was chromatographically distinct and had approximately equal levels of activity in the presence of Mn2+ and Mg2+ . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
We have completed immunofluorescence binding studies of purified fimbriae from two clones which express Pap or Pap 2 fimbriae . ^^^ Although the two fimbrial types exhibited common binding to the uroepithelia of the bladder and renal pelvis and to occasional cells located within the glomeruli which we have termed glomerular elements , only Pap 2 fimbriae adhered to Bowman ' s capsule . ^^^ Results of our experiments demonstrate that this disaccharide is not sufficient for blocking binding of Pap 2 fimbriae to Bowman ' s capsule but that GalNAc beta 1 3Gal completely blocks Pap 2 adherence to Bowman ' s capsule . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The following correlations between the cytological classification and DNA hybridisation were obtained : 55 out of 58 women ( 94 . 8 % ) having a normal Pap smear ( Pap 2 ) were negative by hybridisation as well , three cases contained HPV 6 or 11 . 59 . 3 % of patients revealing abnormalities characteristic of a papillomavirus infection reacted with the 32P labelled DNA , while HPV 6 / 11 or 16 / 18 , respectively , occurred at approximately the same rate of frequency . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Resulting IC 50 values of 3 7 nM PAP , PAP 2 , PAP S or dodecandrin indicate that supernatants from these Phytolacca cells lack a ribosomal protective element . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PAP 3 belongs to the family of pancreatitis associated proteins , recently characterized as pancreatic secretory proteins structurally related to C type lectins , and whose expression is induced during the acute phase of pancreatitis . ^^^ In this paper , we describe the cloning , characterization and chromosomal localization of the rat PAP 3 gene . ^^^ The 5 ' end of the coding sequence was determined by primer extension of the PAP 3 transcript . ^^^ The PAP 3 coding sequence spanned over six exons . ^^^ We found striking similarities between PAP 3 and PAP 1 and 2 genes , in genomic organization as well as in promoter sequences . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Bacteria with either type of P fimbriae were able to colonize but pap 2+ bacteria showed increased colonizing capacity when strains containing pap 1 or pap 2 genes on low copy plasmids were compared . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
A second poly ( A ) polymerase ( PAP 2 ) has been identified in Escherichia coli using a strain carrying a deletion of pcnB ( the structural gene for PAP 1 ; Cao and Sarkar , 1992b ) and pnp 7 ( a null mutation in the structural gene for polynucleotide phosphorylase ) . ^^^ While PAP 1 has a M ( r ) of 53 , 000 , PAP 2 is a smaller protein with a native M ( r ) 35 , 000 . ^^^ PAP 2 differs from PAP 1 in preferring poly ( A ) over tRNA primers and being more thermolabile . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Following the Bethesda guidelines , 2 . 9 % 14 . 7 % of all smears initially reported as Pap 2 K ( suggestive of HPV infection ) would be qualified as risk lesions ( low grade squamous intraepithelial lesions ) , although they tested HPV negative in more than a third of cases . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
There was a high frequency of squamous cell abnormalities ( 2 % Pap 2 , 2 . 5 % Pap 3a , 0 . 5 % Pap 3b or worse ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
We identified and sequenced for the first time a complete cDNA encoding the pokeweed antiviral protein 2 ( PAP 2 ) , which is expressed in the late summer leaves of pokeweed . ^^^ The cDNA of PAP 2 consists of 1 , 187 nucleotides and encodes a mature protein of 285 amino acids . ^^^ The cDNA of PAP 2 was expressed in E . coli . ^^^ Furthermore , PAP 2 , which was produced in E . coli , inhibited protein synthesis in a rabbit reticulocyte translation system . ^^^ Thus , recombinant PAP 2 would appear to be as functional as native PAP in inhibiting protein synthesis in both prokaryotes and eukaryotes . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
This second PAP , which was called PAP 2 , was the same size as the original PAP ( PAP 1 ) and showed 74 . 3 % amino acid homology . ^^^ Studies on gene expression demonstrated that PAP 2 mRNA concentration increased within 6 h following induction of pancreatitis , reached maximal levels ( > 200 times control values ) at 24 48 h , and decreased thereafter , similar to PAP 1 . ^^^ However , PAP 2 mRNA could not be detected in the intestinal tract or in other tissues . ^^^ We also isolated a PAP 2 genomic DNA fragment which was characterized over 2 . 7 kb of gene sequence and 1 . 9 kb of 5 ' flanking sequence . ^^^ The 5 ' end of the coding sequence was determined by primer extension of the PAP 2 mRNA . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The rat genes encoding the pancreatitis associated proteins 1 , 2 and 3 ( Pap 1 , Pap 2 , Pap 3 ) , and the lithostathin / pancreatic stone protein / regeneration protein ( Reg ) colocalize at 4q33 > q 34 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PAP 3 would encode a truncated protein that lacks the NLSs and the S / T rich region . ^^^ The structure of the gene indicates that different forms of PAP are produced by alternative splicing ( PAPs 1 and 2 ) or by competition between polyadenylation and splicing ( PAP 3 ) . ^^^ Mouse PAP 3 and two additional truncated forms , PAPs 5 and 6 , which would be produced by use of poly ( A ) sites in adjacent introns , were also isolated from a mouse brain cDNA library . ^^^ RNase protection and reverse transcription PCR analyses showed that PAP 2 , 5 , and 6 are expressed in all tissues tested but that PAP 1 and / or 4 and 3 are tissue specific . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
One clone cross reacts with PAP S isolated from seeds but none cross reacts with the isoform PAP 2 isolated from summer leaves . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
In this report , we describe the disruption and identification of a candidate gene for a second poly ( A ) polymerase ( PAP 2 ) by an experimental strategy which was based on the assumption that the viability of E . coli depends on the presence of either PAP 1 or PAP 2 . ^^^ E . coli PAP 2 has no significant sequence homology either to PAP 1 or to the viral and eukaryotic poly ( A ) polymerases , suggesting that the bacterial poly ( A ) polymerases have evolved independently . ^^^ An interesting feature of the PAP 2 sequence is the presence of sets of two paired cysteine and histidine residues that resemble the RNA binding motifs seen in some other proteins . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Purification and characterization of N ethylmaleimide insensitive phosphatidic acid phosphohydrolase ( PAP 2 ) from rat liver . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PA is hydrolysed to diacylglycerol by PA phosphohydrolase ( PAP ) which exists in two forms : PAP 1 and PAP 2 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Its predicted amino acid sequence is more similar to PAP ( 76 % ) than to PAP 2 ( 31 % ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The summer leaf isoform of the pokeweed ( Phytolacca americana ) antiviral protein , PAP 2 , was produced in high yields from inclusion bodies in recombinant E . coli . ^^^ PAP 2 variants mutated in residues Asp 88 ( D88N ) , Tyr 117 ( Y117S ) , Glu 172 ( E172Q ) , Arg 175 ( R175H ) and a combination of Asp 88 and Arg 175 ( D88N / R175H ) were produced in E . coli and assayed for their ability to inhibit protein synthesis in a rabbit reticulocyte lysate . ^^^ All of these mutations had effects deleterious to the enzymatic activity of PAP 2 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
A second activity which was insensitive to N ethylmaleimide ( PAP 2 ) was found in the 105 , 000 10 g membrane fraction . ^^^ PAP 2 was inhibited by concentrations of Mg2+ known to occur in ischaemia . ^^^ Specific activities of PAP 1 and PAP 2 in ventricle muscle homogenates were similar . ^^^ The specific activity of PAP 2 in homogenates of cardiac myocytes was only 42 % of that in homogenates of ventricle muscle . 2 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
This enzyme exists in at least two isoforms , one of which ( PAP 1 ) is presumed to be cytosolic and membrane associated and the other ( PAP 2 ) to be an integral membrane protein . ^^^ Two isoforms , presumed to be alternative splice variants from a single gene , designated as PAP 2 alpha1 and PAP 2 alpha2 , have been cloned and expressed . ^^^ The PAP 2 alpha1 and PAP 2 alpha2 have a 84 % and a 72 % overall match , respectively , with the published mouse PAP amino acid sequence . ^^^ Ectopic expression of PAP 2 alpha1 and PAP 2 alpha2 cDNAs in ECV 304 endothelial cells led to a 6 to 8 fold and a 2 fold increase in PAP activity , respectively , in cell free extracts using an in vitro assay that measured the conversion of [ 14C ] PA to [ 14C ] DG . ^^^ The increase in PAP activity in PAP 2 alpha transfected cells correlated with a > 50 % decrease in the steady state PA level . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
In bell pepper , a gene encoding a major plastid lipid associated protein is expressed as both partially and totally spliced transcripts ( respectively PAP 2 and PAP 1 ) . ^^^ Although PAP is present as a single copy gene in the bell pepper genome , Southern blots using PAP 2 as a probe revealed multiple homologous copies . ^^^ Analyses of the intronic sequence of PAP 2 showed the existence of a 206bp short interspersed repetitive element ( SINE ) belonging to the Ts family of retrotransposons ( Yoshioka et al . , 1993 ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Pokeweed antiviral protein isoforms PAP 1 , PAP 2 , and PAP 3 depurinate RNA of human immunodeficiency virus ( HIV ) 1 . ^^^ Our findings presented herein provide direct evidence that three different PAP isoforms from Phytolacca americana ( PAP 1 from spring leaves , PAP 2 from early summer leaves , and PAP 3 from late summer leaves ) cause concentration dependent depurination of genomic RNA ( 63 to 400 pmols of adenine released per micrograms of RNA ) purified from human immunodeficiency virus type 1 ( HIV 1 ) , plant virus ( tobacco mosaic virus ( TMV ) , and bacteriophage ( MS 2 ) . ^^^ PAP 1 , PAP 2 , and PAP 3 ( but not RTA ) inhibited the replication of HIV 1 in human peripheral blood mononuclear cells with IC ( 50 ) values of 17 nM , 25 nM , and 16 nM , respectively . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Members of the type 2 phosphatidic acid phosphatase ( PAP 2 ) family catalyse the dephosphorylation of phosphatidic acid ( PA ) , lysophosphatidate and sphingosine 1 phosphate . ^^^ Here , we demonstrate the presence of a Mg ( 2+ ) independent and N ethymaleimide insensitive PAP 2 activity in cultured guinea pig airway smooth muscle ( ASM ) cells . ^^^ Two PAP 2 cDNAs of 923 and 926 base pairs were identified and subsequently cloned from these cells . ^^^ Transient expression of gpPAP2a2 in Cos 7 cells resulted in an approx . 4 fold increase in Mg ( 2+ ) independent PAP activity , thereby confirming that gpPAP2a2 is another catalytically active member of an extended PAP 2 family . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PAP 1 interacts with itself , PAP 2 , PAP 3 , and lithostathine / regIalpha . ^^^ PAP 1 , PAP 2 , PAP 3 , and lithostathine / regIalpha are members of a multigenic family of proteins expressed in several tissues . ^^^ Because polymerization might regulate activity , we examined the ability of rat PAP 1 to interact with itself ( homodimerization ) , PAP 2 , PAP 3 , and lithostathine / regIalpha ( heterodimerization ) by the yeast two hybrid system , affinity experiments , and crosslinking . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
It was observed that HPV infections from the high risk group occurred more frequently in pap 3 and pap 4 test results ; HPV infections from the low risk group occurred more frequently in pap 1 and pap 2 results , while mixed infections from both risk groups occurred particularly in pap 4 and pap 5 results . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Based on NEM sensitivity we have also demonstrated that the PAPase present in ROS is the PAP 2 isoform . ^^^ Our findings therefore suggest that light inhibition of PAP 2 in ROS is a transducin mediated mechanism . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Previous studies performed in lung have demonstrated the existence of 2 different forms of PAPases , namely PAP 1 and PAP 2 . ^^^ PAP 2 activity was selectively assayed using NEM in the absence of Mg+2 . ^^^ Studies employing this assay and adult rat lung microsomal preparations demonstrated that PAP 2 activity was inhibited by amphiphilic amines , sphingoid bases , products of the PAP 2 reaction ( monoacylglycerol [ MAG ] and diacylglycerol [ DAG ] ) , and substrate analogs such as lysophosphatidic acid ( lyso PA ) , ceramide 1 phosphate , and to a lesser extent , sphingosine 1 phosphate . ^^^ Purified lung plasma membranes , prepared using discontinuous sucrose and Percoll gradients , showed that PAP 2 activity was enriched 6 . 9 + / 1 . 6 fold over the whole homogenate and was between the enrichment for plasma membrane markers , 5 ' nucleotidase ( 14 . 7 + / 0 . 3 ) and Na+ , K ( + ) ATPase ( 4 . 0 + / 0 . 2 ) . ^^^ Both phosphatidic acid and lysophosphatidic acid were good substrates for PAP 2 activity in this purified plasma membrane fraction . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Although one of these lines exhibited a 27 fold increase in PAP 2 activity measured in vitro , levels of PA were not significantly reduced in comparison with control cells . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The amino acid sequence of TPAP contained 642 residues , and shared a high degree of identity ( 86 % ) with that of a nuclear poly ( A ) polymerase , PAP 2 . ^^^ Despite the sequence conservation of functional elements , including three catalytic Asp residues , an ATP binding site , and an RNA binding domain , TPAP lacked an approximately 100 residue C terminal sequence carrying one of two bipartite type nuclear localization signals , and part of a Ser / Thr rich domain found in PAP 2 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Trypsin activation of recombinant stress proteins or counterparts contained in rat pancreatic juice ( PSP / reg , PAP 1 and PAP 3 ) resulted in conversion of 16 kDa soluble proteins into 14 kDa soluble isoforms ( pancreatic thread protein and pancreatitis associated thread protein , respectively ) that rapidly polymerize into insoluble sedimenting structures . ^^^ In contrast , PAP 2 , following activation with trypsin or pancreatic juice , does not form insoluble structures and is rapidly digested by pancreatic proteases . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
However , the length of the identified mRNA ( 1 , 467 base pairs ) was longer than that of rat PAP 2 mRNA ( 885 base pairs ) , because the elongated mRNA was generated through the different polyadenylation site in the same gene . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Twenty two ( 10 . 5 % ) patients showed normal ( Pap 1 ) cytology , 149 ( 71 . 6 % ) patients had inflammation ( Pap 2 ) , and 28 patients ( 13 . 4 % ) had a Pap 3 score . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The percentage of women with a Pap 2 smear ( mild dysplasia ) decreased from 10 % in 1992 to 2 % in 1998 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Pap 1 and Pap 2 are more similar to each other ( 84 % amino acid sequence identity ) than to Pap 3 ( 46 % and 44 % , respectively ) in the encoded mature proteins . ^^^ Pap 1 transcript was most abundant in the maturing anthers ( tapetum ) and in lesser amounts in leaves , fruit coats , seeds , and sepals ; Pap 2 transcript was abundant only in the petals ; and Pap 3 transcript had a wide distribution , but at minimal levels in numerous organs . ^^^ Immunoblotting after sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP 1 or PAP 2 per milligram of total protein , the highest amounts being in the anthers ( 10 . 9 microg 10 mg ( 1 ) PAP 1 ) and petals ( 6 . 6 microg 10 mg ( 1 ) PAP 2 ) , and that they had much less PAP 3 ( < 0 . 02 microg 10 mg ( 1 ) ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The about 100 residues at the C terminal region of a nuclear poly ( A ) polymerase , PAP 2 , were missing in both PAPT proteins . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Two forms of mammalian PAPase can be indentified on the basis of subcellular localization and enzyme properties , one involved predominantly in lipid synthesis ( PAP 1 ) and the other in signal transduction ( PAP 2 ) . ^^^ Our results demonstrate that aging caused PAP 2 to increase in brain microsomal fractions but did not affect PAP 1 , whereas in brain cytosolic fractions , it caused a strong decrease in PAP 1 ( 57 % ) . ^^^ PAP 2 activity in liver microsomal fractions from aged rats showed no changes with respect to adult rats whereas PAP 1 activity increased 228 % in microsomal fractions and 76 % in cytosolic fractions in this tissue . ^^^ However , PA modified utilization during aging through PAP 2 activity could be related to alterations in neural signal transduction mechanisms . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Coordinate regulation of secretory stress proteins ( PSP / reg , PAP 1 , PAP 2 , and PAP 3 ) in the rat exocrine pancreas during experimental acute pancreatitis . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
This enzyme , designated prophenoloxidase activating proteinase 2 ( PAP 2 ) , differs from another PAP , previously isolated from integuments of the same insect ( PAP 1 ) . ^^^ PAP 2 contains two clip domains at its amino terminus and a catalytic domain at its carboxyl terminus , whereas PAP 1 has only one clip domain . ^^^ Purified PAP 2 cleaved prophenoloxidase at Arg ( 51 ) but yielded a product that has little phenoloxidase activity . ^^^ PAP 2 mRNA was not detected in naive larval fat body or hemocytes , but it became abundant in these tissues after the insects were injected with bacteria . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PAP 2 is a six transmembrane domain integral protein localized to the plasma membrane . ^^^ Because PAP 2 degrades sphingosine 1 phosphate ( S1P ) and ceramide 1 phosphate in addition to PA and lyso PA , it has been renamed lipid phosphate phosphohydrolase ( LPP ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Wait and see policy versus loop excision after two consecutive Pap 2 cervical smears : over time less surgery and an equivalent outcome ; no substantial contribution to be expected from the detection of high risk human papillomavirus ] . ^^^ OBJECTIVE : To determine the number interventions and outcomes in patients referred with two consecutive Pap 2 cervical smear results who were managed either by a wait and see policy or aggressively , and to determine whether triage with high risk human papillomavirus ( hr HPV ) detection , resulting in the referral of only hr HPV positive patients , would lead to the detection of all patients with cervical intraepithelial neoplasia ( CIN ) . ^^^ METHOD : 282 patients referred in 1997 / ' 99 with 2 consecutive Pap 2 cervical smears in the screening program were included . ^^^ After the follow up period there was no statistically significant difference between the two groups in terms of the number of patients with persisting Pap 2 : 16 ( 11 % ) versus 12 ( 8 % ) . ^^^ CONCLUSION : The wait and see approach led to fewer interventions , while the number of women with persisting Pap 2 smears was not higher than with the aggressive approach . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
The lower molecular weight fraction was separated into a trypsin inhibitor ( designated Trp 1 ) and early ( designated Pap 1 ) and late ( designated Pap 2 ) eluting papain inhibitors after anion exchange ( Hitrap SP ) chromatography . ^^^ For Pap 2 , two inhibitory peaks ( designated Pap 2 1 and Pap 2 2 ) were identified after further anion exchange ( Resource S ) chromatography . ^^^ Amino acid composition data were obtained from Trp 1 , Pap 1 , Pap 2 2 , and HMWPap 2 2 , and N terminal sequence data from Trp 1 , Pap 2 1 , Pap 2 2 , and HMWPap 2 2 , with two of these sequences ( Pap 2 2 and HMWPap 2 2 ) perfectly matching predicted protein sequences based on EST sequences from an apple database . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Furthermore , thermograms of such cervical lesions as endocervical polyp ( 3 patients ) , erosion ( PAP 2 ) ( 11 cases ) , CIN 1 3 ( 9 patients ) and invasive cancer ( 6 patients ) were studied . ^^^ Statistically significant differences were found in thermographic profiles of the portion between the normal cervix and such cervical lesions as endocervical polyp , erosion ( PAP 2 ) , CIN and invasive cancer . 3 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
On thyrotropin releasing hormone ( TRH ) metabolism , pyroglutamyl aminopeptidase 2 ( PAP 2 ) , a zinc dependent ectoenzyme primarily located in the central nervous system , is believed to play a predominant role . ^^^ The Km values toward TRH by the recombinant PAP Is were about 0 . 05 mM , being similar value to the reported value of recombinant PAP 2 . ^^^ The L pGlu cleaving activities toward TRH in rat brain homogenate were inhibited by a PAP 2 specific inhibitor 1 , 10 phenanthroline , but not inhibited by the antibody against rat PAP 1 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
On the other hand , PAP 2 and DGL 2 activities were observed in all subcellular fractions except in the cytosolic fraction . ^^^ PAP 2 and DGL 2 were increased in the microsomal fraction by 87 and 114 % , respectively , and they were decreased in the nuclear fraction . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Prophenoloxidase activating proteinase 3 ( PAP 3 ) from Manduca sexta hemolymph : a clip domain serine proteinase regulated by serpin 1J and serine proteinase homologs . ^^^ We previously isolated PAP 1 from integument and PAP 2 from hemolymph of the tobacco hornworm , Manduca sexta . ^^^ Here , we report the purification , characterization , and regulation of PAP 3 from the hemolymph . ^^^ Similar to M . sexta PAP 2 , PAP 3 consists of two amino terminal clip domains followed by a carboxyl terminal catalytic domain , whereas PAP 1 contains only one clip domain at its amino terminus . ^^^ Purified PAP 3 cleaved proPO at Arg 51 and generated a low level of PO activity . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Four cryptic plasmids pAP 1 , pAP 2 , pAP 3 , and pAP 4 with their replication regions AP were isolated from Gram negative bacteria Acetobacter pasteurianus 2374 and characterised by sequence analyses . ^^^ Three of four plasmids pAP 2 , pAP 3 , and pAP 4 encode an enzyme that confers ampicillin resistance to host cells . ^^^ Moreover , the tetracycline resistance gene was identified only in pAP 2 plasmid . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
It was shown that recovery of correct performance after errors at deeper drowsiness was accompanied by two types of EEG phasic activation patterns ( PAP 1 and PAP 2 ) . ^^^ The alpha frequency component was always present in both PAP 1 and PAP 2 . ^^^ PAP 2 were recorded at deeper stages and consisted of K complexes with superposition of PAP 1 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Partially purified proteases , PAP 1 and PAP 2 showed a high molecular mass of about 200 kDa by gelatin zymography . ^^^ PAP 1 and PAP 2 had optimal pH from 7 to 9 and were inhibited by trypsin inhibitors . ^^^ These properties of PAP 1 and PAP 2 suggest that both enzymes belong to the membrane associated trypsin like serine protease family , such as enteropeptidase and corin . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Prevalence of human papillomavirus infection in Slovenian women with repeated Pap 2 smears ] . ^^^ Management of patients with repeat abnormal smears ( Pap 2 ) represents a great and complex clinical and public health problem . ^^^ In the study we enrolled 148 cervical samples of women who within two years had three subsequent Pap 2 smears ( abnormal squamous cells or mildly dyskaryotic cells ) . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
In addition to four rat family members ( RegI , Reg 2 / PAP 1 , PAP 2 , and PAP 3 ) that had been identified , we newly cloned and sequenced a type 4 Reg gene in rats . ^^^ Furthermore , a marked induction of PAP 3 mRNA was observed in the distal part of the injured nerve . ^^^ A polyclonal antibody was raised against PAP 3 , and a Western blotting analysis using this antibody confirmed an increased level of PAP 3 protein in the injured nerve . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
PCR yielded fragments encoding seven known ( HP 1 HP4 , PAP 1 , PAP 2 and PAP 3 ) and 18 unknown ( HP 5 HP22 ) serine proteinases . ^^^ Fourteen of the HPs contain regulatory clip domain ( s ) at their amino terminus HP 1 , HP 2 , HP 6 , HP 8 , HP 13 , HP 17 , HP 18 , HP 21 , HP 22 and PAP 1 have one , whereas HP 12 , HP 15 , PAP 2 and PAP 3 have two clip domains . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
EUS FNA was performed in 24 patients revealing PAP 3 ( n = 1 ) , PAP 2 ( n = 21 ) and PAP 1 ( n = 2 ) scores . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
In the periods of 13 24 and of seven to 12 months before the diagnosis of cervical cancer , almost half the patients had Pap 2 , and three to six months before diagnosis , 67 . 6 % of patients had Pap 2 . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
HPV genotypes in repeated PAP 2 smears of group of women in Slovenia ] . ^^^ In our study , we enrolled HPV infected women with repeated Pap smears ( Pap 2 ) in Slovenia and verified HPV infection . ^^^ Using group specific primers PGMY11 / PGMY09 and seven restriction endonucleases in PCR RFLP method for genotypization of HPV we identified single types of HPV included in large percentage of multiple infections of young women with repeated Pap 2 smears . . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
Two MYB transcription factor genes , PAP 1 and PAP 2 , were identified as senescence associated genes that are induced by glucose . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
When transformed with plasmid pAP 2 encoding the K . pneumoniae genes aglB and aglA ( an alpha glucoside specific transporter AglA ( IICB ( Agl ) ) ) , strain MG 1655 ( pAP 2 ) metabolized a variety of other alpha linked glucosides , including maltitol , isomaltose , and the following five isomers of sucrose : trehalulose alpha ( 1 > 1 ) , turanose alpha ( 1 > 3 ) , maltulose alpha ( 1 > 4 ) , leucrose alpha ( 1 > 5 ) , and palatinose alpha ( 1 > 6 ) . ^^^ Remarkably , MG 1655 ( pAP 2 ) failed to metabolize sucrose alpha ( 1 > 2 ) . ^^^ However , when transformed with pTSGH 11 ( encoding ptsG ) or pAP 2 , this organism provided membranes that contained either the PtsG or AglA transporters , respectively . ^^^
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12429 and Q06141	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Cytological follow up and histological results of 2336 patients with Pap 3 and 10584 with Pap 2 control of 1973 and 1974 were checked up to May 1976 and compared with new literature . 50 % of Pap 3 became to negatives , 10 % of Pap 3 and 0 , 82 % of Pap 2 control became positive ( Pap 4 and Pap 5 ) . ^^^ Up to now histological findings has been resulted in 10 % invasive cancers ; in 53 % carcinomata in situ and severe dysplasia , in 16 % moderate and mild dysplasias , and in 5 % normal epithelium from repeated Pap 3 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The isolated proteins ( PAP 1 a , PAP 1 b and PAP 2 ) were homogeneous in various methods including 7 . 5 % and 20 % gel electrophoresis or gel chromatography , and share , in the extract , 85 % of the total of the acidic proteins that migrate with the bromophenol blue marker in 7 . 5 % gels . ^^^ These two proteins were identified to be the nervous system specific protein S 100 by immunochemical and electrophoresis methods as well as by amino acid analysis , and the other protein PAP 2 was revealed to be a calcium binding protein . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PAP 2 activity was insensitive to N ethylmaleimide and did not require Mg2+ for activity . ^^^ Chronic treatment with ethanol increased the activity of PAP 2 in the hearts of the corpulent males , but had no effect in the corpulent females . ^^^ The specific activity of PAP 2 was higher in subcutaneous , gonadal and peri renal adipose depots in the females and in the peri renal depot of the corpulent males compared with the lean genotypes . ^^^ The tissue specificity and the sex differences in the specific activities of PAP 1 and PAP 2 are discussed in terms of their proposed functions in glycerolipid biosynthesis and signal transduction . ^^^ It is proposed that a decreased activity of PAP 2 could be involved in the insulin insensitivity in the corpulent rats . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
To evaluate the changed policy regarding cervical smears with mild atypia in the Netherlands ( before 1988 classified as Pap 2 , thereafter as Pap IIIA ) , an descriptive study was carried out in the laboratory of gynaecological cytology Cyt U Universitair ( University of Utrecht ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The patients with PAP 2 smears had a high incidence of HPV ( 67 % ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Antibodies to PAP 3 detected PAP 3 and inositol 1 , 2 cyclic phosphate 2 phosphohydrolase with identical reactivity on immunoblotting . ^^^ Lipocortin 3 has also been previously identified as placental anticoagulant protein 3 ( PAP 3 ) and calcimedin 35 alpha . ^^^ Lipocortin 3 has also been previously identified as placental anticoagulant protein 3 ( PAP 3 ) and calcimedin 35 alpha . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Of the 109 smears positive for Chlamydia trachomatis , 90 showed cervical cells with reactive changes ( out of 1 , 490 smears with PAP 2 ) , whereas no cytological changes were found in 15 cases ( out of 884 smears with PAP 1 ) . ^^^ Changes suggestive of mild or moderate dysplasia were found in only four cases ( out of 93 smears with PAP 3 ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
From the cytologically normal smears ( Pap 1 , 2 , IIw ) 57 / 424 ( 13 % ) 16 / 18 were found HPV positive and from the pathological ones ( Pap 3 , IIID , IVa , IVb , 5 ) , 30 / 92 ( 33 % ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
NE PAP 1 had chromatographic properties similar to those of S 100 PAP , and both enzymes displayed higher activities in the presence of Mn2+ than in the presence of Mg2+ , whereas NE PAP 2 was chromatographically distinct and had approximately equal levels of activity in the presence of Mn2+ and Mg2+ . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
We have completed immunofluorescence binding studies of purified fimbriae from two clones which express Pap or Pap 2 fimbriae . ^^^ Although the two fimbrial types exhibited common binding to the uroepithelia of the bladder and renal pelvis and to occasional cells located within the glomeruli which we have termed glomerular elements , only Pap 2 fimbriae adhered to Bowman ' s capsule . ^^^ Results of our experiments demonstrate that this disaccharide is not sufficient for blocking binding of Pap 2 fimbriae to Bowman ' s capsule but that GalNAc beta 1 3Gal completely blocks Pap 2 adherence to Bowman ' s capsule . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The following correlations between the cytological classification and DNA hybridisation were obtained : 55 out of 58 women ( 94 . 8 % ) having a normal Pap smear ( Pap 2 ) were negative by hybridisation as well , three cases contained HPV 6 or 11 . 59 . 3 % of patients revealing abnormalities characteristic of a papillomavirus infection reacted with the 32P labelled DNA , while HPV 6 / 11 or 16 / 18 , respectively , occurred at approximately the same rate of frequency . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
These include the monomeric enzymes PAP , PAP S , PAP 2 , and dodecandrin and the enzymatic A chains from the heterodimeric toxins ricin and modeccin . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
In 8 . 3 % of the cases the results were obtained through PAP 3 according to the scheme of Papanicolaou . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The pokeweed antiviral protein , previously identified in two forms ( PAP and PAP 2 ) in the leaves of Phytolacca americana ( pokeweed ) [ Obrig . ^^^ PAP and PAP 2 . 3 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
This comparison showed an accuracy of 64 . 5 % and if the PAP 3 findings were included , of 76 . 6 % in diagnosing prostatic carcinoma by the cytological method . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
BACKGROUND : The pancreatitis associated protein ( PAP ) 1 and 3 , but not PAP 2 , mRNAs are constitutively expressed in the small intestine of rats . ^^^ METHODS : We studied expression of both PAP 1 and PAP 3 mRNAs during development and on nutritional and hormonal manipulations . ^^^ Finally , whereas thyroidectomy and ovariectomy induced a decrease in both mRNA concentrations , and adrenalectomy a limited decrease in PAP 3 mRNA only , diabetes and castration did not alter the expression of either gene . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
We have previously reported the coding sequence of the rat PAP 2 mRNA . ^^^ We show in this paper the existence in rat pancreas of two forms of PAP 2 mRNA with identical coding sequence but a different 5 ' untranslated region . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PAP 3 belongs to the family of pancreatitis associated proteins , recently characterized as pancreatic secretory proteins structurally related to C type lectins , and whose expression is induced during the acute phase of pancreatitis . ^^^ In this paper , we describe the cloning , characterization and chromosomal localization of the rat PAP 3 gene . ^^^ The 5 ' end of the coding sequence was determined by primer extension of the PAP 3 transcript . ^^^ The PAP 3 coding sequence spanned over six exons . ^^^ We found striking similarities between PAP 3 and PAP 1 and 2 genes , in genomic organization as well as in promoter sequences . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Bacteria with either type of P fimbriae were able to colonize but pap 2+ bacteria showed increased colonizing capacity when strains containing pap 1 or pap 2 genes on low copy plasmids were compared . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
A second poly ( A ) polymerase ( PAP 2 ) has been identified in Escherichia coli using a strain carrying a deletion of pcnB ( the structural gene for PAP 1 ; Cao and Sarkar , 1992b ) and pnp 7 ( a null mutation in the structural gene for polynucleotide phosphorylase ) . ^^^ While PAP 1 has a M ( r ) of 53 , 000 , PAP 2 is a smaller protein with a native M ( r ) 35 , 000 . ^^^ PAP 2 differs from PAP 1 in preferring poly ( A ) over tRNA primers and being more thermolabile . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Following the Bethesda guidelines , 2 . 9 % 14 . 7 % of all smears initially reported as Pap 2 K ( suggestive of HPV infection ) would be qualified as risk lesions ( low grade squamous intraepithelial lesions ) , although they tested HPV negative in more than a third of cases . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
There was a high frequency of squamous cell abnormalities ( 2 % Pap 2 , 2 . 5 % Pap 3a , 0 . 5 % Pap 3b or worse ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The rat genes encoding the pancreatitis associated proteins 1 , 2 and 3 ( Pap 1 , Pap 2 , Pap 3 ) , and the lithostathin / pancreatic stone protein / regeneration protein ( Reg ) colocalize at 4q33 > q 34 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PAP 3 would encode a truncated protein that lacks the NLSs and the S / T rich region . ^^^ The structure of the gene indicates that different forms of PAP are produced by alternative splicing ( PAPs 1 and 2 ) or by competition between polyadenylation and splicing ( PAP 3 ) . ^^^ Mouse PAP 3 and two additional truncated forms , PAPs 5 and 6 , which would be produced by use of poly ( A ) sites in adjacent introns , were also isolated from a mouse brain cDNA library . ^^^ RNase protection and reverse transcription PCR analyses showed that PAP 2 , 5 , and 6 are expressed in all tissues tested but that PAP 1 and / or 4 and 3 are tissue specific . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
One clone cross reacts with PAP S isolated from seeds but none cross reacts with the isoform PAP 2 isolated from summer leaves . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
We identified and sequenced for the first time a complete cDNA encoding the pokeweed antiviral protein 2 ( PAP 2 ) , which is expressed in the late summer leaves of pokeweed . ^^^ The cDNA of PAP 2 consists of 1 , 187 nucleotides and encodes a mature protein of 285 amino acids . ^^^ The cDNA of PAP 2 was expressed in E . coli . ^^^ Furthermore , PAP 2 , which was produced in E . coli , inhibited protein synthesis in a rabbit reticulocyte translation system . ^^^ Thus , recombinant PAP 2 would appear to be as functional as native PAP in inhibiting protein synthesis in both prokaryotes and eukaryotes . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Resulting IC 50 values of 3 7 nM PAP , PAP 2 , PAP S or dodecandrin indicate that supernatants from these Phytolacca cells lack a ribosomal protective element . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
This second PAP , which was called PAP 2 , was the same size as the original PAP ( PAP 1 ) and showed 74 . 3 % amino acid homology . ^^^ Studies on gene expression demonstrated that PAP 2 mRNA concentration increased within 6 h following induction of pancreatitis , reached maximal levels ( > 200 times control values ) at 24 48 h , and decreased thereafter , similar to PAP 1 . ^^^ However , PAP 2 mRNA could not be detected in the intestinal tract or in other tissues . ^^^ We also isolated a PAP 2 genomic DNA fragment which was characterized over 2 . 7 kb of gene sequence and 1 . 9 kb of 5 ' flanking sequence . ^^^ The 5 ' end of the coding sequence was determined by primer extension of the PAP 2 mRNA . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Its predicted amino acid sequence is more similar to PAP ( 76 % ) than to PAP 2 ( 31 % ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
A second activity which was insensitive to N ethylmaleimide ( PAP 2 ) was found in the 105 , 000 10 g membrane fraction . ^^^ PAP 2 was inhibited by concentrations of Mg2+ known to occur in ischaemia . ^^^ Specific activities of PAP 1 and PAP 2 in ventricle muscle homogenates were similar . ^^^ The specific activity of PAP 2 in homogenates of cardiac myocytes was only 42 % of that in homogenates of ventricle muscle . 2 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
This enzyme exists in at least two isoforms , one of which ( PAP 1 ) is presumed to be cytosolic and membrane associated and the other ( PAP 2 ) to be an integral membrane protein . ^^^ Two isoforms , presumed to be alternative splice variants from a single gene , designated as PAP 2 alpha1 and PAP 2 alpha2 , have been cloned and expressed . ^^^ The PAP 2 alpha1 and PAP 2 alpha2 have a 84 % and a 72 % overall match , respectively , with the published mouse PAP amino acid sequence . ^^^ Ectopic expression of PAP 2 alpha1 and PAP 2 alpha2 cDNAs in ECV 304 endothelial cells led to a 6 to 8 fold and a 2 fold increase in PAP activity , respectively , in cell free extracts using an in vitro assay that measured the conversion of [ 14C ] PA to [ 14C ] DG . ^^^ The increase in PAP activity in PAP 2 alpha transfected cells correlated with a > 50 % decrease in the steady state PA level . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
In this report , we describe the disruption and identification of a candidate gene for a second poly ( A ) polymerase ( PAP 2 ) by an experimental strategy which was based on the assumption that the viability of E . coli depends on the presence of either PAP 1 or PAP 2 . ^^^ E . coli PAP 2 has no significant sequence homology either to PAP 1 or to the viral and eukaryotic poly ( A ) polymerases , suggesting that the bacterial poly ( A ) polymerases have evolved independently . ^^^ An interesting feature of the PAP 2 sequence is the presence of sets of two paired cysteine and histidine residues that resemble the RNA binding motifs seen in some other proteins . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Purification and characterization of N ethylmaleimide insensitive phosphatidic acid phosphohydrolase ( PAP 2 ) from rat liver . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PA is hydrolysed to diacylglycerol by PA phosphohydrolase ( PAP ) which exists in two forms : PAP 1 and PAP 2 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
In bell pepper , a gene encoding a major plastid lipid associated protein is expressed as both partially and totally spliced transcripts ( respectively PAP 2 and PAP 1 ) . ^^^ Although PAP is present as a single copy gene in the bell pepper genome , Southern blots using PAP 2 as a probe revealed multiple homologous copies . ^^^ Analyses of the intronic sequence of PAP 2 showed the existence of a 206bp short interspersed repetitive element ( SINE ) belonging to the Ts family of retrotransposons ( Yoshioka et al . , 1993 ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The summer leaf isoform of the pokeweed ( Phytolacca americana ) antiviral protein , PAP 2 , was produced in high yields from inclusion bodies in recombinant E . coli . ^^^ PAP 2 variants mutated in residues Asp 88 ( D88N ) , Tyr 117 ( Y117S ) , Glu 172 ( E172Q ) , Arg 175 ( R175H ) and a combination of Asp 88 and Arg 175 ( D88N / R175H ) were produced in E . coli and assayed for their ability to inhibit protein synthesis in a rabbit reticulocyte lysate . ^^^ All of these mutations had effects deleterious to the enzymatic activity of PAP 2 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Pokeweed antiviral protein isoforms PAP 1 , PAP 2 , and PAP 3 depurinate RNA of human immunodeficiency virus ( HIV ) 1 . ^^^ Our findings presented herein provide direct evidence that three different PAP isoforms from Phytolacca americana ( PAP 1 from spring leaves , PAP 2 from early summer leaves , and PAP 3 from late summer leaves ) cause concentration dependent depurination of genomic RNA ( 63 to 400 pmols of adenine released per micrograms of RNA ) purified from human immunodeficiency virus type 1 ( HIV 1 ) , plant virus ( tobacco mosaic virus ( TMV ) , and bacteriophage ( MS 2 ) . ^^^ PAP 1 , PAP 2 , and PAP 3 ( but not RTA ) inhibited the replication of HIV 1 in human peripheral blood mononuclear cells with IC ( 50 ) values of 17 nM , 25 nM , and 16 nM , respectively . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Members of the type 2 phosphatidic acid phosphatase ( PAP 2 ) family catalyse the dephosphorylation of phosphatidic acid ( PA ) , lysophosphatidate and sphingosine 1 phosphate . ^^^ Here , we demonstrate the presence of a Mg ( 2+ ) independent and N ethymaleimide insensitive PAP 2 activity in cultured guinea pig airway smooth muscle ( ASM ) cells . ^^^ Two PAP 2 cDNAs of 923 and 926 base pairs were identified and subsequently cloned from these cells . ^^^ Transient expression of gpPAP2a2 in Cos 7 cells resulted in an approx . 4 fold increase in Mg ( 2+ ) independent PAP activity , thereby confirming that gpPAP2a2 is another catalytically active member of an extended PAP 2 family . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Based on NEM sensitivity we have also demonstrated that the PAPase present in ROS is the PAP 2 isoform . ^^^ Our findings therefore suggest that light inhibition of PAP 2 in ROS is a transducin mediated mechanism . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Although one of these lines exhibited a 27 fold increase in PAP 2 activity measured in vitro , levels of PA were not significantly reduced in comparison with control cells . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The amino acid sequence of TPAP contained 642 residues , and shared a high degree of identity ( 86 % ) with that of a nuclear poly ( A ) polymerase , PAP 2 . ^^^ Despite the sequence conservation of functional elements , including three catalytic Asp residues , an ATP binding site , and an RNA binding domain , TPAP lacked an approximately 100 residue C terminal sequence carrying one of two bipartite type nuclear localization signals , and part of a Ser / Thr rich domain found in PAP 2 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Trypsin activation of recombinant stress proteins or counterparts contained in rat pancreatic juice ( PSP / reg , PAP 1 and PAP 3 ) resulted in conversion of 16 kDa soluble proteins into 14 kDa soluble isoforms ( pancreatic thread protein and pancreatitis associated thread protein , respectively ) that rapidly polymerize into insoluble sedimenting structures . ^^^ In contrast , PAP 2 , following activation with trypsin or pancreatic juice , does not form insoluble structures and is rapidly digested by pancreatic proteases . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PAP 1 interacts with itself , PAP 2 , PAP 3 , and lithostathine / regIalpha . ^^^ PAP 1 , PAP 2 , PAP 3 , and lithostathine / regIalpha are members of a multigenic family of proteins expressed in several tissues . ^^^ Because polymerization might regulate activity , we examined the ability of rat PAP 1 to interact with itself ( homodimerization ) , PAP 2 , PAP 3 , and lithostathine / regIalpha ( heterodimerization ) by the yeast two hybrid system , affinity experiments , and crosslinking . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
It was observed that HPV infections from the high risk group occurred more frequently in pap 3 and pap 4 test results ; HPV infections from the low risk group occurred more frequently in pap 1 and pap 2 results , while mixed infections from both risk groups occurred particularly in pap 4 and pap 5 results . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The percentage of women with a Pap 2 smear ( mild dysplasia ) decreased from 10 % in 1992 to 2 % in 1998 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Pap 1 and Pap 2 are more similar to each other ( 84 % amino acid sequence identity ) than to Pap 3 ( 46 % and 44 % , respectively ) in the encoded mature proteins . ^^^ Pap 1 transcript was most abundant in the maturing anthers ( tapetum ) and in lesser amounts in leaves , fruit coats , seeds , and sepals ; Pap 2 transcript was abundant only in the petals ; and Pap 3 transcript had a wide distribution , but at minimal levels in numerous organs . ^^^ Immunoblotting after sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP 1 or PAP 2 per milligram of total protein , the highest amounts being in the anthers ( 10 . 9 microg 10 mg ( 1 ) PAP 1 ) and petals ( 6 . 6 microg 10 mg ( 1 ) PAP 2 ) , and that they had much less PAP 3 ( < 0 . 02 microg 10 mg ( 1 ) ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The about 100 residues at the C terminal region of a nuclear poly ( A ) polymerase , PAP 2 , were missing in both PAPT proteins . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Two forms of mammalian PAPase can be indentified on the basis of subcellular localization and enzyme properties , one involved predominantly in lipid synthesis ( PAP 1 ) and the other in signal transduction ( PAP 2 ) . ^^^ Our results demonstrate that aging caused PAP 2 to increase in brain microsomal fractions but did not affect PAP 1 , whereas in brain cytosolic fractions , it caused a strong decrease in PAP 1 ( 57 % ) . ^^^ PAP 2 activity in liver microsomal fractions from aged rats showed no changes with respect to adult rats whereas PAP 1 activity increased 228 % in microsomal fractions and 76 % in cytosolic fractions in this tissue . ^^^ However , PA modified utilization during aging through PAP 2 activity could be related to alterations in neural signal transduction mechanisms . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Coordinate regulation of secretory stress proteins ( PSP / reg , PAP 1 , PAP 2 , and PAP 3 ) in the rat exocrine pancreas during experimental acute pancreatitis . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
However , the length of the identified mRNA ( 1 , 467 base pairs ) was longer than that of rat PAP 2 mRNA ( 885 base pairs ) , because the elongated mRNA was generated through the different polyadenylation site in the same gene . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Twenty two ( 10 . 5 % ) patients showed normal ( Pap 1 ) cytology , 149 ( 71 . 6 % ) patients had inflammation ( Pap 2 ) , and 28 patients ( 13 . 4 % ) had a Pap 3 score . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
This enzyme , designated prophenoloxidase activating proteinase 2 ( PAP 2 ) , differs from another PAP , previously isolated from integuments of the same insect ( PAP 1 ) . ^^^ PAP 2 contains two clip domains at its amino terminus and a catalytic domain at its carboxyl terminus , whereas PAP 1 has only one clip domain . ^^^ Purified PAP 2 cleaved prophenoloxidase at Arg ( 51 ) but yielded a product that has little phenoloxidase activity . ^^^ PAP 2 mRNA was not detected in naive larval fat body or hemocytes , but it became abundant in these tissues after the insects were injected with bacteria . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PAP 2 is a six transmembrane domain integral protein localized to the plasma membrane . ^^^ Because PAP 2 degrades sphingosine 1 phosphate ( S1P ) and ceramide 1 phosphate in addition to PA and lyso PA , it has been renamed lipid phosphate phosphohydrolase ( LPP ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Wait and see policy versus loop excision after two consecutive Pap 2 cervical smears : over time less surgery and an equivalent outcome ; no substantial contribution to be expected from the detection of high risk human papillomavirus ] . ^^^ OBJECTIVE : To determine the number interventions and outcomes in patients referred with two consecutive Pap 2 cervical smear results who were managed either by a wait and see policy or aggressively , and to determine whether triage with high risk human papillomavirus ( hr HPV ) detection , resulting in the referral of only hr HPV positive patients , would lead to the detection of all patients with cervical intraepithelial neoplasia ( CIN ) . ^^^ METHOD : 282 patients referred in 1997 / ' 99 with 2 consecutive Pap 2 cervical smears in the screening program were included . ^^^ After the follow up period there was no statistically significant difference between the two groups in terms of the number of patients with persisting Pap 2 : 16 ( 11 % ) versus 12 ( 8 % ) . ^^^ CONCLUSION : The wait and see approach led to fewer interventions , while the number of women with persisting Pap 2 smears was not higher than with the aggressive approach . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Prophenoloxidase activating proteinase 3 ( PAP 3 ) from Manduca sexta hemolymph : a clip domain serine proteinase regulated by serpin 1J and serine proteinase homologs . ^^^ We previously isolated PAP 1 from integument and PAP 2 from hemolymph of the tobacco hornworm , Manduca sexta . ^^^ Here , we report the purification , characterization , and regulation of PAP 3 from the hemolymph . ^^^ Similar to M . sexta PAP 2 , PAP 3 consists of two amino terminal clip domains followed by a carboxyl terminal catalytic domain , whereas PAP 1 contains only one clip domain at its amino terminus . ^^^ Purified PAP 3 cleaved proPO at Arg 51 and generated a low level of PO activity . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
In addition to four rat family members ( RegI , Reg 2 / PAP 1 , PAP 2 , and PAP 3 ) that had been identified , we newly cloned and sequenced a type 4 Reg gene in rats . ^^^ Furthermore , a marked induction of PAP 3 mRNA was observed in the distal part of the injured nerve . ^^^ A polyclonal antibody was raised against PAP 3 , and a Western blotting analysis using this antibody confirmed an increased level of PAP 3 protein in the injured nerve . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
EUS FNA was performed in 24 patients revealing PAP 3 ( n = 1 ) , PAP 2 ( n = 21 ) and PAP 1 ( n = 2 ) scores . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
In the periods of 13 24 and of seven to 12 months before the diagnosis of cervical cancer , almost half the patients had Pap 2 , and three to six months before diagnosis , 67 . 6 % of patients had Pap 2 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
HPV genotypes in repeated PAP 2 smears of group of women in Slovenia ] . ^^^ In our study , we enrolled HPV infected women with repeated Pap smears ( Pap 2 ) in Slovenia and verified HPV infection . ^^^ Using group specific primers PGMY11 / PGMY09 and seven restriction endonucleases in PCR RFLP method for genotypization of HPV we identified single types of HPV included in large percentage of multiple infections of young women with repeated Pap 2 smears . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Furthermore , thermograms of such cervical lesions as endocervical polyp ( 3 patients ) , erosion ( PAP 2 ) ( 11 cases ) , CIN 1 3 ( 9 patients ) and invasive cancer ( 6 patients ) were studied . ^^^ Statistically significant differences were found in thermographic profiles of the portion between the normal cervix and such cervical lesions as endocervical polyp , erosion ( PAP 2 ) , CIN and invasive cancer . 3 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
On the other hand , PAP 2 and DGL 2 activities were observed in all subcellular fractions except in the cytosolic fraction . ^^^ PAP 2 and DGL 2 were increased in the microsomal fraction by 87 and 114 % , respectively , and they were decreased in the nuclear fraction . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Partially purified proteases , PAP 1 and PAP 2 showed a high molecular mass of about 200 kDa by gelatin zymography . ^^^ PAP 1 and PAP 2 had optimal pH from 7 to 9 and were inhibited by trypsin inhibitors . ^^^ These properties of PAP 1 and PAP 2 suggest that both enzymes belong to the membrane associated trypsin like serine protease family , such as enteropeptidase and corin . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Prevalence of human papillomavirus infection in Slovenian women with repeated Pap 2 smears ] . ^^^ Management of patients with repeat abnormal smears ( Pap 2 ) represents a great and complex clinical and public health problem . ^^^ In the study we enrolled 148 cervical samples of women who within two years had three subsequent Pap 2 smears ( abnormal squamous cells or mildly dyskaryotic cells ) . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The phosphohydrolase activity , which is insensitive to N ethylmaleimide ( PAP 2 ) , is specifically located in the plasma membrane ( Jamal , Z . , Martin , A . , Gomez Muoz , A . and Brindley , D . ^^^ Saturated fatty acids , albumin , sphingosine and propranolol have no significant effects on PAP 2 activity . ^^^ However , chlorpromazine decreases PAP 2 activity by about 14 % . ^^^ Linolenate , arachidonate and eicosapentaenoate at 1 mM also produced small ( 7 10 % ) decreases in PAP 2 activity . ^^^ It is proposed that both PAP 1 and PAP 2 activities may be involved in signal transduction , although the main function of PAP 1 seems to be involved in the synthesis of glycerolipids . . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Previous studies performed in lung have demonstrated the existence of 2 different forms of PAPases , namely PAP 1 and PAP 2 . ^^^ PAP 2 activity was selectively assayed using NEM in the absence of Mg+2 . ^^^ Studies employing this assay and adult rat lung microsomal preparations demonstrated that PAP 2 activity was inhibited by amphiphilic amines , sphingoid bases , products of the PAP 2 reaction ( monoacylglycerol [ MAG ] and diacylglycerol [ DAG ] ) , and substrate analogs such as lysophosphatidic acid ( lyso PA ) , ceramide 1 phosphate , and to a lesser extent , sphingosine 1 phosphate . ^^^ Purified lung plasma membranes , prepared using discontinuous sucrose and Percoll gradients , showed that PAP 2 activity was enriched 6 . 9 + / 1 . 6 fold over the whole homogenate and was between the enrichment for plasma membrane markers , 5 ' nucleotidase ( 14 . 7 + / 0 . 3 ) and Na+ , K ( + ) ATPase ( 4 . 0 + / 0 . 2 ) . ^^^ Both phosphatidic acid and lysophosphatidic acid were good substrates for PAP 2 activity in this purified plasma membrane fraction . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
The lower molecular weight fraction was separated into a trypsin inhibitor ( designated Trp 1 ) and early ( designated Pap 1 ) and late ( designated Pap 2 ) eluting papain inhibitors after anion exchange ( Hitrap SP ) chromatography . ^^^ For Pap 2 , two inhibitory peaks ( designated Pap 2 1 and Pap 2 2 ) were identified after further anion exchange ( Resource S ) chromatography . ^^^ Amino acid composition data were obtained from Trp 1 , Pap 1 , Pap 2 2 , and HMWPap 2 2 , and N terminal sequence data from Trp 1 , Pap 2 1 , Pap 2 2 , and HMWPap 2 2 , with two of these sequences ( Pap 2 2 and HMWPap 2 2 ) perfectly matching predicted protein sequences based on EST sequences from an apple database . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Four cryptic plasmids pAP 1 , pAP 2 , pAP 3 , and pAP 4 with their replication regions AP were isolated from Gram negative bacteria Acetobacter pasteurianus 2374 and characterised by sequence analyses . ^^^ Three of four plasmids pAP 2 , pAP 3 , and pAP 4 encode an enzyme that confers ampicillin resistance to host cells . ^^^ Moreover , the tetracycline resistance gene was identified only in pAP 2 plasmid . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
Two MYB transcription factor genes , PAP 1 and PAP 2 , were identified as senescence associated genes that are induced by glucose . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
When transformed with plasmid pAP 2 encoding the K . pneumoniae genes aglB and aglA ( an alpha glucoside specific transporter AglA ( IICB ( Agl ) ) ) , strain MG 1655 ( pAP 2 ) metabolized a variety of other alpha linked glucosides , including maltitol , isomaltose , and the following five isomers of sucrose : trehalulose alpha ( 1 > 1 ) , turanose alpha ( 1 > 3 ) , maltulose alpha ( 1 > 4 ) , leucrose alpha ( 1 > 5 ) , and palatinose alpha ( 1 > 6 ) . ^^^ Remarkably , MG 1655 ( pAP 2 ) failed to metabolize sucrose alpha ( 1 > 2 ) . ^^^ However , when transformed with pTSGH 11 ( encoding ptsG ) or pAP 2 , this organism provided membranes that contained either the PtsG or AglA transporters , respectively . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
It was shown that recovery of correct performance after errors at deeper drowsiness was accompanied by two types of EEG phasic activation patterns ( PAP 1 and PAP 2 ) . ^^^ The alpha frequency component was always present in both PAP 1 and PAP 2 . ^^^ PAP 2 were recorded at deeper stages and consisted of K complexes with superposition of PAP 1 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
On thyrotropin releasing hormone ( TRH ) metabolism , pyroglutamyl aminopeptidase 2 ( PAP 2 ) , a zinc dependent ectoenzyme primarily located in the central nervous system , is believed to play a predominant role . ^^^ The Km values toward TRH by the recombinant PAP Is were about 0 . 05 mM , being similar value to the reported value of recombinant PAP 2 . ^^^ The L pGlu cleaving activities toward TRH in rat brain homogenate were inhibited by a PAP 2 specific inhibitor 1 , 10 phenanthroline , but not inhibited by the antibody against rat PAP 1 . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
PCR yielded fragments encoding seven known ( HP 1 HP4 , PAP 1 , PAP 2 and PAP 3 ) and 18 unknown ( HP 5 HP22 ) serine proteinases . ^^^ Fourteen of the HPs contain regulatory clip domain ( s ) at their amino terminus HP 1 , HP 2 , HP 6 , HP 8 , HP 13 , HP 17 , HP 18 , HP 21 , HP 22 and PAP 1 have one , whereas HP 12 , HP 15 , PAP 2 and PAP 3 have two clip domains . ^^^
Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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Interacting proteins: Q06141 and P12429	Pubmed	SVM Score :0.0
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