Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.64045558
Co immunoprecipitation analysis showed that the association between NR 1 and NR2A was decreased in the trapped animals . 0.64045558^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.51957091
NR 1 was co associated with NR2A , NR2B and NR2D but not NR2C , nor was NR2C detected in adult mouse hippocampal membranes . 0.51957091^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunological detection of the NMDAR 1 glutamate receptor subunit expressed in embryonic kidney 293 cells and in rat brain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Upon expression in cultured cells , the new subunits yielded prominent , typical glutamate and NMDA activated currents only when they were in heteromeric configurations with NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The half maximal response ( ED 50 ) value of NAAG on expressed NMDAR 1 was 185 microM , which shows that NAAG is about 115 times less potent than L glutamate ( Glu ) , the ED 50 of which value was 1 . 6 microM . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus , we examined immunocytochemically the distribution of the common NMDAR 1 glutamate receptor subunit to evaluate further the potential sites of glutamatergic action . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of the gene coding for the NR 1 subunit of the N methyl D aspartate ( NMDA ) type of glutamate receptor was investigated in the developing rat brain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of the NR 1 subunit in Xenopus oocytes produces channels that respond to glutamate and are blocked by competitive and noncompetitive antagonists of the N methyl D aspartate ( NMDA ) receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Nitric oxide ( NO ) , an important neuromessenger , and the glutamate receptor subtype , NMDA NR 1 , have been implicated in nociception in the STN . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The order of affinity for [ 3H ] glutamate was NR 1 NR2B > NR 1 NR2A approximately NR 1 NR2D > NR 1 NR2C > NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Antibodies to the NMDAR 1 glutamate receptor subunit and the GluR 1 and GluR2 / 3 subunits of the AMPA glutamate receptor were used to localize these receptor components in the basolateral amygdala ( ABL ) of the rat and monkey . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Because N methyl D aspartate ( NMDA ) glutamate ionotropic receptors are involved in the occurrence of audiogenic seizures , we investigated by in situ hybridization methods , the expression of the different subunits ( NR 1 , NR2A , NR2B , NR2C ) of this receptor in the central nucleus of the inferior colliculus ( IC ) , a main relay of the auditory pathways . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In order to characterize the neurochemical profile of these neoplastic and malformative glioneuronal lesions , we have examined the presence of the alpha 1 subunit of the GABAA receptor , the N methyl D aspartate receptor subunit 1 ( NR 1 ) , glutamate decarboxylase , tyrosine hydroxylase , somatostatin , parvalbumin , and calretinin in 60 gangliogliomas , 11 DNT , 10 tuberous sclerosis like lesions and 17 non tuberous sclerosis like glioneuronal malformations . ^^^ Despite a great variation between and within the different entities , the neurochemical profile was generally reminiscent of normal neocortex : glutamate decarboxylase , GABAA receptor and NR 1 which are common in neocortical neurons were present in the great majority of the lesions and often showed high labeling indices . ^^^ There were three tuberous sclerosis like lesions ( 30 % ) that contained both NR 1 and glutamate decarboxylase immunoreactive giant cells in addition to well differentiated ganglion cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Messenger RNA levels for the N methyl D aspartate receptor subunit , NR 1 ; the non N methyl D aspartate receptor subunits , glutamate receptors 3 and 4 ; the high affinity kainate receptor subunits 1 and 2 ; and the GABAA receptor subunits , alpha 2 , beta 2 , gamma 2 were unchanged . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The high evolutionary conservation in the structure and properties of NMDAR 1 argues strongly for the importance of this receptor in functions of glutamate neurotransmission . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Regulation of the expression of NR 1 NMDA glutamate receptor subunits during hippocampal LTP . ^^^ Several isoforms of the NMDA receptor 1 ( NR 1 ) subunits of the ionotropic NMDA ( N methyl D aspartate ) glutamate receptor contain a consensus site for phosphorylation by protein kinase C ( PKC ) which , once phosphorylated results in an increased conductance through the receptor channel . ^^^ The change in the expression of PKC sensitive NR 1 subunits may be the molecular basis for the increased response of the post synaptic cell to released glutamate during the maintenance phase of LTP . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The glutamate receptor channels of the N methyl D aspartate ( NMDA ) subtype are composed of different subunits named NR 1 and NR2A D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Nine multiplex schizophrenia families were genotyped with polymorphisms for the GLUR 5 and NMDAR 1 glutamate receptor subunit genes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The immunocytochemical localization of N acetylaspartyl glutamate , its hydrolysing enzyme NAALADase , and the NMDAR 1 receptor at a vertebrate neuromuscular junction . ^^^ Here we describe the immunocytochemical detection at the rat neuromuscular junction of N acetylaspartylglutamate in terminals of motoneurons , of N acetylated alpha linked acidic dipeptidase in perisynaptic Schwann cells , and of the NMDAR 1 glutamate receptor subunit on postsynaptic muscle membranes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Comparison of intron positions in genes encoding different members of the glutamate receptor family confirms a close evolutionary relationship of the NR2C and NMDAR 1 subunit genes . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA receptor channel is formed by at least two members of the glutamate receptor ( GluR ) channel subunit families , the GluR epsilon ( NR 2 ) and GluR zeta ( NR 1 ) subunit families . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
High affinity binding to NR 1 transfected cells was not observed with the transmitter recognition site radioligands L [ 3H ] glutamate and D , L ( epsilon ) 2 [ 3H ] amino 4 propyl 5 phosphono 3 pentanoic acid ( [ 3H ] CGP 39653 ) or the ion channel radioligand [ 3H ] dizocilpine ( [ 3H ] MK 801 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The N methyl D aspartate ( NMDA ) NR 1 glutamate receptor subtype has been proposed to play an important role in the transmission of orofacial sensory information in the spinal trigeminal nucleus ( STN ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI phosphate when present in equimolar concentration with NRII . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have used site directed mutagenesis to study amino acids in the human NR 1 subunit that contribute to the glycine binding site of the NMDA receptor without affecting the agonist site for glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Fourteen glutamate receptor subunits were studied : GluR 1 4 ( including flip and flop variants ) , GluR 5 7 , KA 1 & 2 , NR 1 , and NR2A D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used in situ hybridization to examine the effect of removal of the glutamatergic input to the striatum by unilateral frontal cortical ablation on the expression of genes encoding subunits from three families of glutamate receptors : N methyl D aspartate receptors ( NMDAR 1 , NMDAR2A , and NMDAR2B ) ; alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) receptors ( GluR 1 4 , flip and flop splice variants ) ; and metabotropic receptors ( mGluR 1 5 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA subtypes of glutamate receptors are formed after coexpression of the NR 1 cDNA with a cDNA of the NR 2 family . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Transcript levels of the NMDA receptor genes NMDAR 1 , NR2A , NR2B , NR2C , and NR2D and a glutamate binding protein ( GBP ) were not altered by kindling . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , in situ hybridization techniques were employed to map the distribution of enteric neurons which express mRNA for the glutamate N methyl D aspartate receptor 1 ( NMDAR 1 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate receptor mRNA for the AMPA subunits GluR 1 , GluR 2 and GluR 4 , and the NMDA receptor subtype NMDAR 1 , were heavily expressed in the SCN and surrounding areas . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
On the basis of sequence similarity and pharmacological properties , the recently cloned glutamate receptor subunits have been assigned as components of NMDA ( NMDAR 1 , 2A D ) , AMPA ( GluR 1 4 ) and KA ( GluR 5 7 , KA 1 , KA 2 ) receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study attempted to explore the gene expression of the subunits ( GluR 1 4 ) of the alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid ( AMPA ) type receptor , subunit ( GluR 5 ) of kainic acid ( KA ) type receptor , NR 1 [ a subunit of N methyl D aspartate ( NMDA ) receptors ] and the possible glutamate binding subunit of an NMDA receptor complex in the dorsal horn of the rat spinal cords using in situ hybridization histochemistry . ^^^ Labelling of the dorsal horn neurons by the GluR 5 probe was low , and NR 1 probe and a glutamate binding subunit of an NMDA receptor complex probe labelled them diffusely with low to moderate intensity . ^^^ However , expression of NR 1 and the glutamate binding subunit of an NMDA receptor complex is very strong . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We examined the effects of chronic ethanol exposure on the levels of N methyl D aspartate receptor subunit 1 ( NMDAR 1 ) protein , an essential component of N methyl D aspartate glutamate receptors , in rat brain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The localization of AMPA receptor subunits ( GluR 1 4 ) , a KA receptor subunit ( GluR 5 ) and NMDA receptor subunits ( NR 1 and NRgbs ; the glutamate binding subunits of an NMDA receptor complex ) was investigated using immunohistochemistry and in situ hybridization histochemistry in the rat dorsal root ganglion . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
With the anti NR 1 A antibody , a 125 kDa protein was discovered that may represent a glutamate receptor not yet characterized . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Comparison with previous studies of localization of other glutamate receptor types revealed that NMDAR 1 may colocalize with these other types in many neurons throughout the nervous system . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These results demonstrate that alternative splicing of the NR 1 messenger RNA regulates its phosphorylation by PKC , and that mRNA splicing is a novel mechanism for regulating the sensitivity of glutamate receptors to protein phosphorylation . ^^^ These results also provide evidence that the C terminal domain of the NR 1 protein is located intracellularly , suggesting that the proposed transmembrane topology model for glutamate receptors may be incorrect . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The deduced amino acid sequence of DNMDAR 1 displays 46 % amino acid identity to the rat NMDAR 1 polypeptide and shows significant homology ( 16 23 % ) to other vertebrate and invertebrate glutamate receptor proteins . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Alternatively spliced isoforms of the NMDAR 1 glutamate receptor subunit : differential expression in the basal ganglia of the rat . ^^^ Several isoforms of the NMDAR 1 glutamate receptor subunit are produced by alternative mRNA splicing of three cassette sequences . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Sections were immunolabeled with antibodies to the neuronal markers NeuN , glutamate , and calbindin D28k , and to receptors for fibroblast growth factor 2 ( FGFR 1 ) and NMDA ( NMDAR 1 ) and were examined using confocal microscopy . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
After NMDAR 1 antisense ODN injection , striatal binding of 3H glutamate target mRNA signal intensity in the injected striatum . ^^^ After NMDAR 1 antisense ODN injection , striatal binding of 3H glutamate to NMDA sites was not altered , although strychnine insensitive 3H glycine binding sites exhibited a small but significant reduction . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Comparative ultrastructural localization of the NMDAR 1 glutamate receptor in the rat basolateral amygdala and bed nucleus of the stria terminalis . ^^^ Our findings provide the first ultrastructural evidence that glutamate may prominently act through NMDAR 1 receptors to elicit postsynaptic actions on intrinsic neurons in the BLA and BNST . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Drugs of abuse and stress increase the expression of GluR 1 and NMDAR 1 glutamate receptor subunits in the rat ventral tegmental area : common adaptations among cross sensitizing agents . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Histamine and the polyamine agonists spermine and spermidine enhanced glutamate toxicity in a dose dependent manner consistent with expression of an NR 1 NR2B combination of subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , the contribution of the M 3 M4 loop of the NR 1 subunit to the binding of glutamate and the co agonist glycine was investigated by site directed mutagenesis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The N methyl D aspartate ( NMDA ) subtype of ionotropic glutamate receptor is assembled from one NR 1 subunit , expressed in eight splice variants , and four NR 2 subunits ( NR2A D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast , neither glutamate nor NMDA treatment caused neuronal degeneration in NR 1 / cells , indicating that NMDA receptors are responsible for rapidly triggered glutamate neurotoxicity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N methyl D aspartate ( NMDA ) type glutamate receptors are constituted of one obligatory subunit ( NR 1 ) , expressed as eight splice variants , combined with one or more of four NMDAR 2 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The current through homomeric NMDAR 1 expressed in Xenopus oocytes was increased by 200 500 % by phorbol ester and also by activation of a metabotropic glutamate receptor ( mGluR 1 ) expressed in the same oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The rank order of potency was quinolinic acid < NMDA < homoquinolinic acid < or = glutamate for the NR 1 + NR2A and NR 1 + NR2B combinations whereas for NR 1 + NR2C it was quinolinic acid < < < < homoquinolinic acid < NMDA < or = glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Oligonucleotide primers were designed from the previously cloned sequences of the AMPA selective glutamate receptors ( GluR 1 4 ) , KA selective glutamate receptors ( GluR 5 7 , KA 1 & 2 ) , NMDA selective glutamate receptors ( NMDAR 1 & 2 ) , and metabotropic glutamate receptors ( mGluR 1 4 ) . ^^^ The present study suggested that the subunits of GluR 1 4 , GluR 5 , and NMDAR 1 may be assembled into the hetero oligomeric glutamate receptor complex in the VG and that the VG neuron specific stoichiometry of the receptor complex may be functionally significant for the afferent signal transduction in the rat peripheral vestibular system . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have examined the expression of the NR 1 subunit of the glutamate NMDA receptor and the immediate early gene c jun in lumbar spinal cord following neonatal common peroneal nerve crush . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Homogenates were immunoblotted by using antibodies directed against glutamate transporters ( GLT 1 , GLAST , and EAAC 1 ) and glutamate receptors ( GluR 1 , GluR2 / 3 , GluR6 / 7 , and NMDAR 1 ) , and they were assayed for glutamate transport by D [ 3H ] aspartate binding . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Transiently catecholaminergic cells in the fetal rat express mRNA for the glutamate NMDAR 1 receptor . ^^^ Whether TC cell migration is glutamate mediated remains unclear , since some of them successfully reached the gut without expressing NMDAR 1 message . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , we used an immunohistochemical double labelling approach to localize ionotropic glutamate receptor subunits ( NMDAR 1 , GluR 1 , GluR2 / 3 , GluR 4 and GluR5 / 6 / 7 ) on specific striatal neuron populations . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution of glutamate receptor subunit proteins GluR 2 ( 4 ) , GluR5 / 6 / 7 , and NMDAR 1 in the canine and primate cerebral cortex : a comparative immunohistochemical analysis . ^^^ The distribution of the AMPA , kainate and NMDA glutamate receptor subunit proteins GluR 2 ( 4 ) , GluR5 / 6 / 7 and NMDAR 1 , respectively , were analyzed in the dog hippocampus and neocortex and compared to macaque monkeys and humans . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The aetiology of ALS is poorly understood , although abnormalities in glutamate and glycine transport have been reported as well as alterations in NMDA receptors including the NR 1 subunit ; suggesting a role for glutamate in the disease process . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The effect of chronic haloperidol treatment on glutamate receptor subunit ( GluR 1 , GluR 2 , KA 1 , KA 2 , NR 1 ) mRNAs and glutamate binding protein mRNA in rat forebrain . ^^^ The mRNAs targetted were the flip and flop isoforms of GluR 1 and GluR 2 , KA 1 and KA 2 , NR 1 , and the glutamate binding protein ( GBP ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
High level expression of the NMDAR 1 glutamate receptor subunit in electroporated COS cells . ^^^ The rat N methyl D aspartate ( NMDA ) glutamate receptor subunit NR 1 1a was transiently expressed in COS cells using the technique of electroporation , which was fivefold more efficient than the calcium phosphate precipitation method of transfection . ^^^ Although immunofluorescent labeling of electroporated COS cells demonstrated that the NR 1 1a protein appears to be associated with the cell membrane , neither NMDA nor glutamate effected an increase in intracellular calcium concentration in fura 2 loaded cells , suggesting that homomeric NR 1 1a receptors do not act as functional ligand gated ion channels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Alternatively , NMDAR 1 subunit splice variants may differentially respond to interactions with glutamate . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the NR 1 subunit of the NMDA receptor , mutation of D 732 to glutamate ( D732E ) , asparagine ( D732N ) , alanine ( D732A ) , or glycine ( D732G ) reduced the potency of glycine by > 4000 fold , but these mutations had no effect on sensitivity to glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
It was found that long term , but not short term , ethanol exposure increased levels of immunoreactivity of the NMDAR 1 subunit , an obligatory component of NMDA glutamate receptors , and of the GluR 1 subunit , a component of many AMPA glutamate receptors ; but at the same time , long term ethanol exposure decreased immunoreactivity levels of the alpha 1 subunit of the GABAA receptor complex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , the distribution of glutamate receptors was examined with preembedding immunocytochemistry , using 14 antibodies to ionotropic ( GluR 1 , GluR2 / 3 , GluR 4 , GluR 5 7 , GluR6 / 7 , KA 2 , NR 1 , NR2A / B , delta 1 / 2 ) and metabotropic ( mGluR 1 alpha , mGluR2 / 3 , mGluR 5 ) glutamate receptor subtypes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of NMDA receptor genes ( NR 1 , NR2A B ) was examined by in situ hybridization using oligonucleotide probes , and binding to NMDA receptors assessed using L [ 3H ] glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To determine whether the perilesional cortex shows evidence for abnormal excitatory or inhibitory neurochemical activity , we immunohistochemically examined the distribution of the alpha 1 subunit of the GABAA receptor ( GABAR ) , the N methyl D aspartate receptor subunit 1 ( NR 1 ) , and glutamate decarboxylase ( GAD ) in 30 surgical specimens of neocortical epilepsy associated lesions . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDAR 1 mRNA expression and glutamate receptor stimulated increase in cytosolic calcium concentration in rat and mouse cerebellar granule cells . ^^^ We tested this hypothesis by measuring ( a ) the concentration of mRNA for the obligatory NMDA receptor subunit , NMDAR 1 , and ( b ) the glutamate / NMDA stimulated increase in cytosolic Ca ( 2+ ) ion concentration in cultures at physiological or elevated K ( + ) ion concentration . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present immunocytochemical study used three specific antibodies to GluR 2 , 4 , GluR 5 , 6 , 7 and to NMDAR 1 to differentiate between the regional distribution of alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid ( AMPA ) , kainate and N methyl D aspartate ( NMDA ) subtypes of glutamate receptors throughout the rabbit spinal cord . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridisation and immunocytochemical studies reveal the presence of several NMDA ( NMDAR 1 , NMDAR2C ) , non NMDA ( GluR 1 , GluR 2 , GluR 4 ) and metabotropic ( mGluR 1 ) glutamate receptor subunits in the SCN . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Ultrastructural localization of glutamate receptor subunits ( NMDAR 1 , AMPA GluR 1 and GluR2 / 3 ) and spinothalamic tract cells . ^^^ The associations of glutamate receptor subunits ( NMDAR 1 , AMPA GluR 1 and GluR2 / 3 ) and spinothalamic tract neurons in the rat lumbar spinal cord dorsal horn were investigated . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The distribution of ionotropic glutamate receptor subunits GluR 1 and NMDAR 1 , and the inhibitory neurotransmitter gamma aminobutyric acid ( GABA ) was studied by immunocytochemistry , in the monkey amygdala . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate activated currents ( evoked every 20 s for up to 20 min ) were increased by src and fyn kinases without affecting the desensitization and deactivation kinetics in NR 1 NR2A but the kinases had no effects in NR 1 NR2B , NR 1 NR2C and NR 1 NR2D receptor channels , suggesting that a phosphorylation site in NR2A is targeted . 4 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The trafficking and phosphorylation of the NR 1 and NR 2 subunits of the N methyl D aspartate type glutamate receptor complex were studied in cultured rat hippocampal neurons . ^^^ Following stimulation of the cultures with glutamate / glycine or phorbol esters , NR 1 phosphorylation was found to be enhanced by 3 5 fold , whereas phosphorylation of NR 2 subunits was enhanced by less than 2 fold . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution of glutamate receptors in these two synapse populations in rat cerebella was studied using preembedding immunocytochemistry with antibodies to GluR 1 , GluR2 / 3 , GluR 5 7 , NR 1 , delta 1 / 2 , and mGluR 1 alpha . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Changes of NMDA receptor subunit ( NR 1 , NR2B ) and glutamate transporter ( GLT 1 ) mRNA expression in Huntington ' s disease an in situ hybridization study . ^^^ The distribution of NMDA receptor subunit ( NR 1 , NR2B ) and glia bound glutamate transporter ( GLT 1 ) mRNAs was investigated in postmortem brains of Huntington ' s disease ( HD ) patients and controls by means of in situ hybridization using radiolabeled deoxyoligonucleotides . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Homology based molecular modeling of the glutamate and glycine binding domains indicates that the NR 2 and NR 1 subunits use similar residues to ligate the agonists ' alpha aminocarboxylic acid groups , whereas differences in side chain interactions and size of aromatic residues determine ligand selectivity . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The combination of NMDAR 1 and NMDAR 2 in heteromeric configurations potentiates glutamate response and produces a functional variability . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Moreover , findings from this study suggest that a change in NR 1 splicing following a neurological injury could significantly alter glutamate pathogenicity in a particular population of cells . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In addition , we show that stimulation of metabotropic glutamate receptor 1alpha , which potentiates NMDA channels through PKC , decreases the ability of NR 1 to bind to calmodulin . ^^^ Thus , our data provide clues to understanding the basis of cross talk between two types of receptors , metabotropic glutamate receptors and the NR 1 subunit , in NMDA channel potentiation . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These neurons also express various glutamate receptor subunits including GluR 1 , GluR 4 and NMDAR 1 as detected by immunological methods . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution of glutamate receptors GluR 2 / 3 and NR 1 in the developing rat cerebellum . ^^^ The distribution of glutamate receptors GluR2 / 3 and NR 1 was analysed immunohistochemically during development of the rat cerebellum . ^^^ The developmental appearance of stellate / basket cells and Golgi cells as well as their high reactivity remaining into adulthood suggest that these inhibitory molecular and granular layer interneurons are the principal targets of glutamate axons serving NR 1 synaptic properties while Purkinje cells and brush type granule cells are targets for glutamate connections with GluR2 / 3 characteristics . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We tested the hypothesis that NMDA receptors are necessary for prenatal development of central neural circuits underlying respiratory rhythm generation by comparing the responsiveness of control mice and mutant mice lacking the NMDA receptor R 1 subunit ( NMDAR 1 ) gene to glutamate receptor agonists and antagonists and comparing endogenous respiratory related oscillations generated in vitro by brain stem spinal cord and medullary slice preparations from control and mutant mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Such treatment caused an increase in the expression of the NMDAR 1 receptor subunit , 15 % above the levels in the pair fed controls , as well as of three subunits of a complex that has properties characteristic of NMDA receptors , the glutamate , carboxypiperazinylphosphonate , and glycine binding proteins . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate immunoreactive neurons were found in cultured myenteric ganglia , and a subset of enteric neurons expressed NMDA ( NR 1 , NR2A / B ) , AMPA ( GluR 1 , GluR2 / 3 ) , and kainate ( GluR5 / 6 / 7 ) receptor subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The distribution of ionotropic glutamate receptor subunits GluR 1 , GluR2 / 3 and NMDAR 1 , and meta botropic receptor mGluR 1 alpha was studied in the monkey substantia nigra . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The distribution of subunits for the N methyl D aspartate ( NR 1 , NR2A / B ) , alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate ( GluR 1 , GluR2 / 3 , GluR 4 ) and low affinity kainate ( GluR5 / 6 / 7 ) ionotropic glutamate receptors was examined by immunocytochemistry in the temporal cortex and hippocampus of the rhesus macaque ( Macaca mulatta ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To better characterize and distinguish the two entities we examined 25 oligodendrogliomas and 16 DNT immunohistochemically for the presence of the proliferation associated Ki 67 antigen and the following neural antigens : the alpha 1 subunit of the GABAA receptor ( GABAR ) , N methyl D aspartate receptor subunit 1 ( NR 1 ) , glutamate decarboxylase , neuronal nuclei antigen ( NeuN ) , the embryonal form of the neural cell adhesion molecule ( E NCAM ) , synaptophysin , neurofilament protein ( NFP ) , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The main findings were as follows : 1 ) AMPA selective GluR2 / 3 and NMDAR 1 share similar distribution patterns in the rat and cat somatosensory thalamus , 2 ) both glutamate receptors are likely to be colocalized at postsynaptic densities of both RL and RS synapses , and 3 ) localization of the glutamate receptor proteins in GABAergic dendrites in the cat thalamus indicates that glutamatergic transmission to GABAergic neurons is also mediated by both NMDA and non NMDA receptors . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Previously it was thought that the binding sites for glycine and glutamate were formed by residues on the NR 1 subunit . ^^^ Therefore we propose that the glutamate binding site is located on NR 2 subunits and ( taking our data together with previous work ) is not on the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate , aspartate , N methyl D aspartate receptor ( NMDAR 1 and 2 subunits ) , and nitric oxide synthase ( NOS ) immunoreactive neurons were studied in the arcuate nucleus ( AN ) of mice treated neonatally with monosodium glutamate ( MSG ) which is known to cause extensive neuronal loss in this hypothalamic nucleus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDAR 1 glutamate receptor subunit isoforms in neostriatal , neocortical , and hippocampal nitric oxide synthase neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemistry with antibodies specific for glutamate receptors GluR 1 , GluR 2 ( 4 ) ( alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ) , GluR5 / 6 / 7 ( kainate ) and NR 1 ( N methyl D aspartate ) receptor subunits demonstrated that virtually all projection neurons in all subfields contained subunits from each receptor class . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The median eminence taken from ovariectomized rats was dual immunostained with GnRH and ionotropic glutamate receptor subtypes ( NR 1 , GIuR 1 , GluR2 / 3 , GluR6 / 7 and KA 2 ) , and their colocalization was examined under electron microscopy . ^^^ Of the glutamate receptor subtypes , NR 1 and KA 2 immunoreactivities were colocalized with GnRH immunoreactivity in nerve terminals of the median eminence . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunostaining for GluR2 / 3 , NR 1 or NR 2 showed , in both MAM and control animals , that glutamate receptors were mainly concentrated in the soma and dendrites of neocortical and hippocampal pyramidal cells , including in heterotopiae , and in the apical dendrites of hippocampal granule cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , the numbers of glycine binding NR 1 and glutamate binding NR 2 subunits in the NMDA receptor hetero oligomer were determined by coexpressing the wild type ( wt ) NR 1 with the low affinity mutant NR 1 ( Q387K ) , and the wt NR2B with the low affinity mutant NR2BE387A , subunits in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In order to understand how specific glutamate receptor genes are involved in the treatment of schizophrenia we have used a multiprobe oligonucleotide solution hybridization ( MOSH ) technique to examine the regulation of gene express of the NMDAR 1 , 2A , 2B , 2C , 2D receptor subunits in the left rat brain following treatment with the optical isomers of flupenthixol . cis and trans flupenthixol are both present in the commonly used oral and depot treatments for schizophrenia and a controlled trial showed that cis flupenthixol had a significantly superior ability to ameliorate the positive symptoms of schizophrenia compared to its trans isomer . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The binding site for the co agonist glycine on N methyl D aspartate ( NMDA ) receptors has been mapped to the NR 1 subunit whereas binding of the principal agonist glutamate is mediated by the NR 2 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We performed a quantitative electron microscopic analysis of the middle third of the molecular layer in the dentate gyrus of rat , using material processed with postembedding gold labeling for the glutamate receptor subunits GluR 1 , GluR2 / 3 , or NMDAR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At the postsynaptic level , immunohistochemistry and Western blotting revealed in the suprachiasmatic nuclei of infected rats a selective decrease of the expression of glutamate AMPA GluR2 / 3 and NMDAR 1 receptor subunits that gate retinal afferents . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemistry was used to study the expressions of glutamate receptor subunit proteins for NMDAR2A / B , NMDAR 1 splice variants , and AMPA Glu R2 / 3 in human brain resected for intractable epilepsy associated with cortical dysplasia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These glycoproteins , purified by concanavalin A lectin affinity chromatography , showed immunoreactivity in the present study with subunit specific antibodies to glutamate receptors as follows : GP 180 , NMDA receptor subunits NR2A / NR2B ; GP 116 , NMDA receptor NR 1 ( 1a ) ; and GP 110 , pan alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( pan AMPA ) receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To address whether neuronal activity may differentially regulate the surface expression at synapses of two specific subtypes of ionotropic glutamate receptors we epitope tagged an AMPA ( alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ) receptor subunit ( GluR 1 ) and an NMDA ( N methyl D aspartate ) receptor subunit ( NR 1 ) on their extracellular termini and expressed these proteins in cultured hippocampal neurons using recombinant adenoviruses . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemical localization of metabotropic glutamate receptors ( mGluRs ) and ionotropic glutamate receptors ( NMDA type : NMDAR 1 and NMDAR2A C ; AMPA type : GluR 1 4 ) was performed on sections of rat dorsal horn . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N terminal splice variants of the NMDAR 1 glutamate receptor subunit : differential expression in human and monkey brain . ^^^ The distribution of the mRNA coding for the two N terminal splice variants of the N methyl D aspartate receptor 1 ( NMDAR 1 ) subunit of the glutamate NMDA receptor was studied on whole hemisphere human and macaca fascicularis brain sections by in situ hybridisation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunolabelling for the AMPA glutamate receptor subunits 1 , 2 / 3 , and 4 ( GluR 1 , GluR2 / 3 , and GluR 4 ) and the NMDA receptor subunit 1 ( NR 1 ) was localized predominantly within asymmetrical synapses in both the EP and STN . ^^^ However , at some NR 1 and GluR2 / 3 positive synapses , the level of glutamate immunoreactivity was low in the presynaptic terminal and , in the STN , some of them were GABA immunopositive . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The glycine binding site of this hetero oligomeric ion channel protein is formed by two distinct extracellular regions , S 1 and S 2 , of the NR 1 subunit , whereas the homologous domains of the NR 2 subunit mediate glutamate binding . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Repeated electroconvulsive seizures ( ECS ) induced isoforms of DeltaFosB in frontal cortex , an effect that was associated with increased levels of the NMDA receptor 1 ( NMDAR 1 ) glutamate receptor subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
H ] MDL 105 , 519 binds with equal high affinity to both assembled and unassembled NR 1 subunits of the NMDA receptor . [ 3H ] MDL 105 , 519 ( ( ( E ) 3 ( 2 phenyl 2 carboxyethenyl ) 4 , 6 dichloro 1 [ 3H ] indole 2 ca rboxylic acid ) is a novel radioligand which binds with high affinity , Kd = 2 . 5 nM , to the glycine site of adult rodent forebrain , N methyl D aspartate subtype of glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N Methyl D aspartate glutamate receptors ( NMDAR ) form ion channels made up of polypeptides from two classes of subunits ; NR 1 is obligatory for function whereas members of the NR 2 class regulate the properties of the channel . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The association of NMDA receptor subunits and CaMKII was assessed by immunoprecipitating PSD proteins with antibodies specific for NR2A / B and CaMKII : CaMKII coprecipitated with NR2A / B and NR 1 but not with other glutamate ionotropic receptor subunits , such as GluR 1 and GluR 2 3 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Each of these recombinant receptor proteins consisting both of NR 1 and NR 2 subunits also showed a specific binding site for the NMDA agonist glutamate when using L [ 3H ] glutamate as a radioligand . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , immunocytochemistry was used to study the expressions of glutamate receptor subunit proteins for NMDAR2A / B , NMDAR 1 and AMPA Glu R2 / 3 in human brain resected for intractable epilepsy associated with cortical dysplasia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate receptor subunit GluR 2 and NMDAR 1 immunoreactivity in the retina of macaque monkeys with experimental glaucoma does not identify vulnerable neurons . ^^^ The relationships between the cellular distribution of glutamate receptor subunit proteins GluR 2 and NMDAR 1 and the vulnerability of restricted retinal neuron subpopulations was explored in experimental glaucoma in macaque monkeys , produced by treating the trabecular meshwork in one eye with argon or diode laser burns . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , the immunohistochemical distribution of alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) selective glutamate receptor subunits ( GluR 1 , GluR2 / 3 , GluR 4 ) and the N methyl D aspartate ( NMDA ) receptor subunit NR 1 in the cat caudal brainstem was investigated by using subunit specific antibodies . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of NMDAR 1 , GluR 1 , GluR 7 , and KA 1 glutamate receptor mRNAs is decreased in frontal cortex of `` neuroleptic free ' ' schizophrenics : evidence on reversible up regulation by typical neuroleptics . ^^^ To test these hypotheses , we measured mRNAs encoding the NMDAR 1 , GluR 1 , GluR 7 , and KA 1 subunits of glutamate receptor in the left superior frontal gyrus from 21 elderly schizophrenics with varying histories of antipsychotic drug treatment and nine normal drug free elderly controls . ^^^ There were significant negative correlations between NMDAR 1 , GluR 1 , GluR 7 , and KA 1 mRNA levels and time without neuroleptic medication before death in schizophrenics , indicating that levels of the glutamate receptor mRNAs decline rapidly after drug withdrawal . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Sections of the substantia nigra were obtained and receptor immunocytochemistry was performed using commercially available antibodies ( against subunits of ionotropic glutamate receptors : GluR 1 , GluR2 / 3 , GluR 4 , NMDAR 1 , NMDAR2A / B ; and subtypes of metabotropic glutamate receptors : mGluR1alpha , mGluR2 / 3 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the immunocytochemical study , immunoreactivity for glutamate or its ionotropic receptor subtypes , such as NR 1 , GluR 1 , GluR2 / 3 , GluR6 / 7 , and KA 2 , was examined in the median eminence of OVX rats under electron microscopy . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Our results demonstrate that DA regulates the phosphorylation state of the NR 1 subunit of NMDA type glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Turnover analysis of glutamate receptors identifies a rapidly degraded pool of the N methyl D aspartate receptor subunit , NR 1 , in cultured cerebellar granule cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of glutamate receptor subunit / subtype messenger RNAS for NMDAR 1 , GLuR 1 , GLuR 2 and mGLuR 5 by accumbal projection neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
An antisense oligodeoxynucleotide which specifically blocked the production of the glutamate receptor subtype NMDAR 1 was administered to the nucleus tractus solitarius ( NTS ) in order to examine the role of this receptor in baroreflex control of heart rate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , we sought to localize glutamate receptor ( GluR ) subunits ( GluR 5 / 6 / 7 , GluR 2 / 3 and N methyl D aspartate receptor 1 [ NMDAR 1 ] ) and a glutamate transporter subtype [ GLT 1 ] within the leech CNS using mono and polyclonal antibodies . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In follow up studies , immunocytochemical and in situ hybridisation analysis of the NMDAR 1 and NMDAR 2 glutamate receptor subunits confirmed their widespread distribution in regions containing GnRH perikarya , but no colocalization within GnRH neurons was observed . ^^^ The resistance of GnRH neurons to the neurotoxic actions of two different glutamate agonists and the failure to detect colocalisation of NMDAR 1 or NMDAR 2 subunits within GnRH perikarya are consistent with the notion that the effects of glutamate upon GnRH secretion are not exerted directly upon GnRH cell bodies . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The effect of treatment with the D 1 dopamine receptor agonist SKF 38393 on the expression of metabotropic glutamate receptor 1 , 3 , 4 and 5 receptor subtypes and of the glutamate N methyl D aspartate ionotropic receptor subunits NRI , NR2A and NR2B was analysed using in situ hybridization . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To further examine the role of N methyl D aspartate ( NMDA ) and alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) glutamate receptors in these responses , double labeling studies of immunoreactivity for c Fos protein and either NMDA ( NR 1 ) or AMPA ( mGluR2 / 3 ) receptor expression were conducted in normoxic rats and in hypoxic rats receiving vehicle , MK 801 , or NBQX . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Since [ 3H ] L 689 , 560 , [ 3H ] CGP 39653 and [ 3H ] ifenprodil label the glycine , glutamate and ifenprodil sites of the NMDA receptor complex , which are associated with NR 1 , NR1 / NR2A and NR1 / NR2B subunits respectively , our findings suggest that NR2B containing receptors are selectively up regulated in superior temporal cortex in schizophrenia . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
By exchanging ion pore domains between functional glutamate receptors ( GluR 1 , GluR 6 and NMDAR 1 ) with known pore properties we first tested the feasibility of the domain swapping method . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The focus of the present study was on the role of Ca2+ entry into neurons , either through receptor or voltage gated channels , in the expression of the NMDA receptor subunit NR 1 and the 71 kDa glutamate binding protein ( GBP ) of a glutamate / NMDA receptor like complex . ^^^ Activation or inhibition of other glutamate ionotropic receptors had no effect on the expression of NR 1 and GBP . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The down regulation of the functional effects of glutamate was dependent on the duration of PDC exposure and was accompanied by a reduced NMDAR 1 subunit expression and decreased [ 3H ] MK 801 binding , indicative of a pronounced structural rearrangement of NMDA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
No GnRH neurons were immunopositive for NR 1 , indicating that effects of glutamate on GnRH neurons are mediated by interneurons or other glutamate receptor subunits or types . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA NR 1 subunit mRNA and glutamate NMDA sensitive binding are differentially affected in the striatum and pre frontal cortex of Parkinson ' s disease patients . ^^^ Changes in the levels of mRNA for the NR 1 subunit of the glutamate NMDA receptor and in NMDA sensitive glutamate binding were investigated in consecutive sections of the prefrontal cortex and striatum of control and Parkinson ' s disease ( PD ) post mortem brain using in situ hybridisation and receptor autoradiography . ^^^ In the striatum , levels of glutamate binding to the NMDA receptor detected by receptor autoradiodgraphy were significantly reduced in the PD group , while no change could be detected at a macroscopical level in NMDA NR 1 mRNA expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This region of NR 1 is most similar to bacterial leucine / isoleucine / valine binding protein , a globular amino acid binding protein containing two lobes , similar to the downstream S 1 S2 region of glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
GluR 1 , GluR2 / 3 , and NMDAR 1 subunits of glutamate receptors were quantified from immunoblots in these brain nuclei in rats at 24 h and 3 weeks after discontinuing 1 week of daily cocaine injections . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA receptor channel is formed from two gene products from two glutamate receptor subunit families , termed NR 1 and NR 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To investigate the role of NMDA receptor subunits in the neuroendocrine functions of the magnocellular neuroendocrine cells of the hypothalamus , we examined the effects of osmotic stimulation on the protein expression of the NMDA receptor subunits , NR 1 and NR2B , important in binding glycine and glutamate , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blot analyses using antibodies against different subunits of 2 amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) and N methyl D aspartate ( NMDA ) types of ionotropic glutamate receptors indicate that AMPA receptor subunits ( GluR 1 , GluR2 / 3 , and GluR 4 ) , but not NMDA receptor subunits ( NR 1 and NR2A ) , are proteolytically cleaved after exposure of hippocampal neurons to apoptotic insults , including Abeta , and that the caspase inhibitor zVAD fmk suppresses such cleavage . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We performed an electron microscopic study of S 1 cortex by using postembedding immunogold histochemistry to examine the subcellular distribution of alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( AMPA ) receptors ( assessed with an antibody recognizing the glutamate receptor 2 and 3 [ GluR 2 and GluR 3 ] subunits ) and to compare this distribution with that of N methyl D aspartate ( NMDA ) receptors ( assessed with an antibody for the NR 1 subunit ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We measured the glutamate induced increase of calcium concentration in fibroblasts expressing one of the NR 1 splice forms and the NR2B subunit . ^^^ The increase of calcium concentration after glutamate application had a positive correlation with the amount of NR 1 splice forms expressed on the cell surface . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study we have used double label immunocytochemistry to analyze the expression of the glutamate receptor subunits GluR 1 , GluR2 / 3 , NR 1 , mGluR 1 , and mGluR 5 in the dopaminergic cells of the striatum . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In vitro studies show that while NR 2 alone is nonfunctional , NR 1 alone produces weak currents to glutamate or NMDA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Localization of alternatively spliced NMDAR 1 glutamate receptor isoforms in rat striatal neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study examined the electrophysiological effects produced by activation of specific dopamine ( DA ) receptors and the distribution of DA receptor subtypes and glutamate receptor subunits [ N methyl D aspartate ( NMDAR 1 ) and GluR 1 ] in cortical tissue samples obtained from children ( ages 3 months to 16 years ) undergoing epilepsy surgery . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have quantitated the protein levels of alpha amino isoxazolepropionic acid ( AMPA ) type ( GluR 1 ) and N methyl D aspartate type ( NMDAR 1 ) glutamate receptors in postmortem brain tissues of patients with Alzheimer ' s disease and age matched controls using western blotting . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The trophic action of chronic NMDA treatment appears to involve a downregulation of glutamate receptors as shown by both a reduction in the obligatory NR 1 subunit protein of the NMDA receptor and a decrease in the kainate induced Co ( 2+ ) uptake in MNs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We conclude that alternative splicing of NR 1 pre mRNA transcripts may be regulated by developmental cues that modulate the degree of glutamate receptor activation . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This is a study of the effect of the unilateral administration of dopamine ( DA ) in the pars compacta of the substantia nigra ( SN ) of the rat on striatal glutamate receptor subunit ( GluR 1 , GluR 2 and NMDAR 1 ) gene expression determined by in situ hybridization . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution of glutamate receptor subunit NMDAR 1 in the hippocampus of normal elderly and patients with Alzheimer ' s disease . ^^^ Notably , the overall pattern of NMDAR 1 immunoreactivity is distinct from that observed with antibodies against AMPA receptor subunits and suggests a differential role of various inotropic glutamate receptors in hippocampal plasticity in AD . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The immunohistochemical localization of N methyl D aspartate ( NMDA ) glutamate receptor subunit , NR 1 was investigated in the chinchilla cristae ampullaris and utricular maculae at the light and electron microscopy level with the use of specific antipeptide antibodies . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Similarly , mRNAs encoding all four alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( AMPA ) type glutamate receptor subunits and the NR 1 and NR2A , 2B , and 2D subunits of the NMDA type glutamate receptors were detected , suggesting that various combinations of these subunits mediate the cellular response to synaptically released glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To define further sites for functional interactions between opiate ligands and excitatory amino acid receptors , the ultrastructural localization of the mu opioid receptor ( MOR ) was examined with respect to either the kainate receptor ( KAR ) or the R 1 subunit of the N methyl D aspartate ( NR 1 ) type glutamate receptor in the LC . ^^^ Gold silver labeling for MOR and peroxidase labeling for either KAR or NR 1 indicated that the MOR often was localized to the plasma membrane of dendrites that also exhibited immunolabeling for either glutamate receptor subtype . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To characterize molecular events that precede cell death following transient focal ischemia , in situ hybridization histochemistry was used to measure levels of glutamate receptor subunit 1 ( GluR 1 ) , GluR 2 , GluR 3 , N methyl D aspartate receptor subunit 1 ( NR 1 ) and preproenkephalin messenger RNAs in adult rats at various recirculation times ( 1 . 5 , 3 and 24 h ) following a 90 min period of middle cerebral artery occlusion . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Double immunostaining revealed that many of the constitutively expressed Zif 268 positive neurons were GABAergic but very few were cholinergic or neuronal nitric oxide synthase ( nNOS ) positive , and some of the Zif 268 positive neurons were also immunopositive for a glutamate NMDA receptor subunit NR 1 or NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of the glutamate receptors has been determined at multiple levels of gene expression in postmortem brain samples from schizophrenics and controls ; while results have not been entirely consistent from study to study , several generalizations have emerged from this literature : ( 1 ) The AMPA receptor is abnormally decreased in expression in the schizophrenic hippocampus , involving decreased levels of subunit transcripts and protein levels , as well as binding sites , ( 2 ) similar changes are seen for kainate receptor expression in the hippocampus , and ( 3 ) the obligate NMDA receptor subunit , NMDAR 1 , may be abnormally expressed in some cortical regions in schizophrenia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Alternative splicing of the NMDAR 1 glutamate receptor subunit in human temporal lobe epilepsy . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The receptor protein expression of glutamate receptors mGluR 1 , GluR 2 , GluR5 / 7 and NMDAR 1 were not modified in response to PCP treatment . 6 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The study was designed to analyze the levels of mRNA coding for NMDAR 1 , the essential subunit of the N methyl D aspartate ( NMDA ) receptor subtype , and for GluR 1 , an AMPA glutamate receptor subunit , after repeated immobilization stress in rat hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , we hypothesized that the expressions of glutamate receptor , NMDA receptors ( NMDAR 1 ) and neuronal nitric oxide synthase ( nNOS ) were colocalized and were also correlated with that of cytochrome oxidase ( CO ) in a subset of neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemical studies demonstrated the presence of the glutamate receptor proteins NMDAR 1 , NMDAR2A / B , GluR 1 , GluR 2 , GluR2 / 3 , GluR 4 , GluR6 / 7 , and KA 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glycine concentration response curves from acutely dissociated hippocampal neurons revealed a 5 and 86 fold reduction in receptor glycine affinity in mice carrying Grin 1 ( D481N ) and Grin 1 ( K483Q ) mutations , respectively , whereas receptor glutamate affinity remained unaffected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This study was aimed to investigate some of the underlying mechanisms of the preconvulsive behavior elicited by unilateral SNpr DA injection by the study of changes in the gene expression of glutamate receptor subunits ( GluR 1 , GluR 2 and NMDAR 1 ) and of changes in animal behavior following coinfusion of DA and a DA D 1 antagonist SCH 23390 into the SNpr . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
CONCLUSIONS : The loss of EAAT 1 in glaucoma may account for the elevated level of glutamate found in glaucomatous vitreous and lead to a compensatory downregulation of NMDAR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In order to investigate the process of gene expression of N methyl D aspartate glutamate receptor ( NMDAR ) subunits in the rat neostriatum and how this relates to motor behaviors , a single dose of antisense phosphodiester oligodeoxynucleotide specific for NMDAR 1 was unilaterally applied in the neostriatum in a stereotaxic apparatus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Alpha actinin ( alpha actinin 2 ) is a protein which links the NR 1 and NR2B subunits of N methyl D aspartate ( NMDA ) glutamate receptors to the actin cytoskeleton . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expressions of AMPA ( GluR 2 , GluR 3 , and GluR 4 ) and NMDA ( NR 1 and NR 2 ) glutamate receptors in the spiral ganglion cells of the cochlea were analyzed both at protein and mRNA levels in control and deaf rats between week 0 ( P 14 ) and week 8 ( P 70 ) after deafness induced by a daily injection of kanamycin at P 0 P14 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This article reviews the diversity that has been found in the subunit composition of GABA , glycine , alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid and N Methyl , D aspartate ( NMDA ) receptors in the mammalian auditory brain stem and provides new data on how the NMDAR 1 glutamate receptor subunit changes as a consequence of deafness . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We also demonstrate that synaptic AMPA and NMDA receptor clusters form with a similar time course during synaptogenesis and that functional AMPA receptors cluster independently of activity and glutamate receptor activation , including following the deletion of the NMDA receptor NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , mRNA for CRH and selected ionotropic glutamate receptor ( iGluR ) subunits ( NR 1 , GluR 2 , GluR 3 ) was quantified in the hippocampus of pigs in which stress was simulated by central administration of CRH ( 100 microg ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Fluctuation analysis of the glutamate evoked current revealed that the NMDAR 1 W 5L mutation increased channel mean open time , providing further evidence for an alteration in gating . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We visualized here the distribution of the major ionotropic glutamate receptors in quail by immunocytochemical methods by using commercial primary antibodies raised against rat glutamate receptor 1 and receptors 2 3 ( GluR 1 , GluR2 / 3 : AMPA subtype , Chemicon , CA ) , rat glutamate receptors 5 7 ( GluR 5 7 : kainate subtype , Pharmingen , CA ) , and rat NMDA receptors ( NMDAR 1 , Pharmingen , CA ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors represent a subtype of the ionotropic glutamate receptor family , comprising three classes of subunits ( NR 1 , NR2A D , NR 3 ) , which exhibit distinct patterns of regional and developmental expression in the CNS . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We are interested in studying the co localization of NMDA glutamate receptor subunits ( NR 1 , NR2A / B ) and AMPA glutamate receptor subunits ( GluR 1 , GluR 2 , GluR2 / 3 and GluR 4 ) in individual neurons of the rat vestibular nuclei . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Dysfunction of N methyl D aspartate ( NMDA ) type ionotropic glutamate receptors has been implicated in the etiology of schizophrenia based on psychotomimetic properties of the antagonist phencyclidine ( PCP ) and observation that mice expressing low levels of the N methyl D aspartate receptor NR 1 subunit exhibit behavioral alterations that may be ameliorated by neuroleptic drugs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Otherwise , the observed diminution of NMDAR 1 1 splice variant mRNA and protein levels may , at least partially , explain the decreased vulnerability of nNOS alpha ( Delta / Delta ) mice to glutamate mediated neurotoxicity . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors , an ionotropic subtype of glutamate receptors ( GluRs ) forming high Ca ( 2+ ) permeable cation channels , are composed by assembly of the GluRzeta subunit ( NR 1 ) with any one of four GluRepsilon subunits ( GluRepsilon 1 4 ; NR2A D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NR 1 gene encodes the major subunit that is responsible for channel function , and NR 1 / mice die at birth , inhibiting the study of glutamate signaling in postnatal neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have stably transfected HEK 293 cells with recombinant human CaBP in order to determine the influence of this protein upon transient increases in intracellular ionic calcium concentration ( [ Ca ( 2+ ) ] ( 1 ) ) induced either by transient transfection of the NR 1 and NR2A subunits of the N methyl D aspartate ( NMDA ) receptor and brief exposure to glutamate , photolysis of the caged calcium compound NP EGTA , or exposure to the Ca ( 2+ ) ] ionophore 4 Br A 23187 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Moreover , there is a significant positive correlation between the levels of conditioning and greater immunoreactivity with the glutamate receptor 4 ( GluR 4 ) AMPA receptor subunit in the abducens motor nuclei , but not with NMDAR 1 or GluR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Sleep recordings , Morris water maze experiments , and immunohistochemistry for NMDA NR 1 glutamate receptor , c fos protein , and apoptosis [ nuclear immunoreactivity for single stranded DNA and terminal deoxynucleotidyl transferase mediated biotinylated UTP nick end labeling assay ] were conducted in EHYP exposed Sprague Dawley male rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Unilateral hypoglossal nerve axotomy was used as a model to analyse immunohistochemically the expression of the GluR 1 , GluR 2 , GluR 3 , and GluR 4 glutamate receptor subunits of the alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) subtype and the NR 1 subunit of the N methyl D aspartate ( NMDA ) subtype in the different morphofunctional hypoglossal pools from 1 to 45 days postaxotomy . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Fos immunostaining was used as a marker of neuronal activity following intracranial self stimulation ( ICSS ) of the medial forebrain bundle ( MFB ) in the rat , and was combined with immunostaining for tyrosine hydroxylase ( TH ) , serotonin ( 5 HT ) , gamma aminobutyric acid ( GABA ) , or NR 1 ( one of the glutamate N methyl D aspartate receptor subunits ) for purposes of neurochemical identification . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To gain a better understanding about the precise sites of action of glutamate and the subunit compositions of the receptors involved in the glutamatergic neurotransmission in the hypothalamus and septum , in situ hybridization was used with 35S labeled cRNA probes for the different ionotropic receptor subunits , including glutamate receptor subunits 1 4 ( GluR 1 GluR4 ) , kainate 2 , GluR 5 GluR7 , N methyl D aspartate ( NMDA ) receptor 1 ( NMDAR 1 ) , and NMDAR2A NMDAR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Furthermore , UBCs identified by calretinin immunoreactivity expressed the glutamate receptor subunits GluR2 / 3 , NMDAR 1 , and mGluR2 / 3 , like they do in the mature mouse cerebellum in situ . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate NMDAR 1 receptors localised to nerves in human Achilles tendons . ^^^ In this investigation , we show the presence of both free glutamate ( microdialysis ) and glutamate NMDAR 1 receptors ( immunohistochemical analyses of tendon biopsies ) , in tendons from patients with chronic Achilles tendon pain ( Achilles tendinosis ) and in controls ( pain free tendons ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The distribution of glutamate receptors in the monkey subthalamic nucleus was studied using affinity purified polyclonal antibodies to GluR 1 , phosphorylated GluR 1 , GluR2 / 3 , NMDAR 1 , mGluR1a and mGluR 5 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Nitric oxide synthase positive neurons in the rat superior colliculus : colocalization of NOS with NMDAR 1 glutamate receptor , GABA , and parvalbumin . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In cells exposed to the glutamate transport blocker , L trans pyrrolidine 2 , 4 dicarboxylate ( PDC ) , the expression of the C 1 exon positive NR 1 mRNA variant was reduced by about 40 % whereas , the expression of C 1 negative mRNA was increased leading to significant reduction of the +C1 / C 1 ratio . ^^^ The expression of the N 1 negative NR 1 variants was slightly reduced following exposure to PDC , indicating that increased medium levels of glutamate changed the relative abundance of NR 1 splice variant expression but did not reduce the overall NR 1 transcription . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Under control conditions , receptors containing mutated NR 1 and NR 2 subunits show much smaller glutamate responses than wild type receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
E2F1 ( + / + ) and ( / ) cultures were comparable , in that they contained similar neuronal densities , responded with similar increases in intracellular calcium concentration ( [ Ca ( 2+ ) ] 1 ) to glutamate receptor agonists , and showed similar NMDA receptor subunit mRNA expression levels for NR 1 , NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In normal control animals , regional differences in the expression of several glutamate receptor subunit / subtypes , e . g . , NR 1 and NR2A subunits of the N methyl D aspartic acid ( NMDA ) receptor , GluR 2 and KA 2 subtypes of non NMDA receptors , and mGluR 1 and mGluR 7 metabotropic glutamate receptors , were consistent with previous results from studies using in situ hybridization histochemistry , suggesting that the real time quantitative PCR method was a reliable procedure for evaluation of changes in mRNA expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N methyl D aspartate receptors ( NRs ) are a group of ionotropic glutamate receptors in the brain and they are composed of heteromeric subunits ( NR 1 , NR2A D and NR 3 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In vivo microdialysis and immunohistochemical analyses of tendon tissue demonstrated high amounts of free glutamate and glutamate NMDAR 1 receptors , but no signs of inflammation , in Jumper ' s knee . ^^^ In the biopsies , there were no inflammatory cell infiltrates , but , for the first time , it was shown that there was immunoreaction for the glutamate receptor NMDAR 1 in association with nerve structures in human patellar tendons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 ( F639A ) did not alter the agonist potency of glutamate but did produce a leftward shift in the glycine concentration response for receptors containing NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The authors used in situ hybridization to determine NMDAR 1 , NMDAR2A NMDAR2D , gluR 1 gluR7 , KA 1 , and KA 2 subunit mRNA levels and receptor autoradiography to determine binding to glutamate binding sites of the three receptor subtypes and to the glycine , polyamine , and ion channel binding sites of the NMDA receptor . ^^^ RESULTS : Glutamate receptor expression was lower at both transcriptional ( NMDAR 1 , NMDAR2B , NMDAR2C , gluR 1 , gluR 3 , and KA 2 subunit mRNAs ) and posttranscriptional ( [ ( 3 ) H ] ifenprodil and [ ( 3 ) H ] MDL 105 , 519 binding to polyamine and glycine sites of the NMDA receptor ) levels in the thalamus in patients with schizophrenia than in comparison subjects , but differences were most prominent in nuclei with reciprocal projections to limbic regions . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Asymmetrical reductions of hippocampal NMDAR 1 glutamate receptor mRNA in the psychoses . ^^^ Using in situ hybridisation histochemistry ( ISHH ) , we measured mRNA for the obligatory NMDAR 1 subunit of the NMDA glutamate receptor in post mortem samples of hippocampus from schizophrenics , depressives , bipolar patients and normal controls . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus , glutamate receptor function can be altered in the mouse by regulated NR 1 ( R ) transgene expression . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To determine the role of the intracellular C terminal tails of glutamate receptor subunits in the synaptic targeting of AMPARs and NMDARs , we fused the tails of the AMPAR subunits , GluR 1 and GluR 2 , and the NMDAR subunit , NR 1 , to the human T lymphocyte membrane protein CD 8 and expressed these constructs in HEK 293 cells and cultured hippocampal neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , the effect of Con G on recombinant NMDA receptors carrying point mutations within the glycine and glutamate binding pockets of the NR 1 and NR2B subunits was studied using whole cell voltage clamp recording from cRNA injected Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Furthermore , glutamate receptor subunit profiles ( GluR 1 , GluR2 / 3 , GluR2 / 4 , GluR5 / 6 / 7 and NMDAR 1 ) were also determined for these cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
When coexpressed with NR 1 and NR2A in heterologous cells , NR3B suppressed glutamate induced current similarly to NR3A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This study was undertaken to characterize the AMPA ( GluR 1 , GluR2 / 3 , and GluR 4 ) , KA ( GluR5 / 6 / 7 ) , and NMDA ( NR 1 ) ionotropic glutamate receptor subunits and to determine their distribution during the development of the chick retina by Western blotting and immunohistochemistry . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have localized regions in the S 1 binding domain of both subunits required for the transmission of allosteric signals from the glutamate binding NR2A subunit to the glycine binding NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The mRNA of the NR 1 subtype of glutamate receptor in Alzheimer ' s disease . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These currents have been generally ascribed to a functional association between NR 1 and the endogenous glutamate receptor subunit XenU 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These effects of InsP 6 appear to be specific to AMPA receptors , as neither 3H glutamate binding to NMDA receptors nor levels of NR 1 and NR2A subunits in P 2 and P 3 fractions were affected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Conventional heteromeric NMDARs composed of NR 1 and NR2A D subunits require dual agonists , glutamate and glycine , for activation . ^^^ Remarkably , when co expressed in Xenopus oocytes , NR3A or NR3B co assembles with NR 1 to form excitatory glycine receptors that are unaffected by glutamate or NMDA , and inhibited by D serine , a co activator of conventional NMDARs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Moreover , we detected an enrichment of glutamate receptors subunits that including NR 1 subunit of NMDA receptors and GluR 1 subunit of AMPA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Age related changes in GluR 2 and NMDAR 1 glutamate receptor subunit protein immunoreactivity in corticocortically projecting neurons in macaque and patas monkeys . ^^^ We performed an immunohistochemical analysis of neurons forming short and long corticocortical projections in young and old monkeys using antibodies to the GluR 2 and NMDAR 1 glutamate receptor subunit proteins . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we describe the use of real time quantitative PCR using a fluorescent TaqMan probe , to study the regional differences in expression of glutamate receptor subunit / subtype genes ( NR 1 , NR2A , GluR 2 , KA 2 , mGluR 1 , mGluR 7 ) in the central vestibular system including the vestibular nucleus complex , inferior olive and cerebellar flocculus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RESULTS : Single dose of morphine was followed by a decrease in gene expression of glutamate receptor subunit NMDAR 1 in the adrenal gland . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To further define the amino acids through which glutamate activates the receptor , we generated single site mutations to the NR2A subunit , and expressed them with wild type NR 1 in HEK 293 cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To elucidate the morphological changes in immunopositive cells of ionotropic glutamate receptors within intrastriatal ' developing ' grafts of fetal ventral mesencephalon ( VM ) in 6 hydroxydopamine lesioned rats , immunohistochemistry was performed to detect cells expressing N methyl D aspartate ( NMDA ) receptor subunit 1 ( NR 1 ) , the alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) receptor subunits ( GluR 1 , GluR2 / 3 , and GluR 4 ) , or tyrosine hydroxylase ( TH ) in the intrastriatal VM grafts at 1 , 4 , and 12 weeks following transplantation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The immunohistochemical localization of the NMDA glutamate receptor subunits NR 1 , NR2A , and NR2B was investigated in the rat vestibular periphery at the light and electron microscopy level using specific antipeptide antibodies . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors are ionotropic glutamate receptors assembled of subunits of the NR 1 and of the NR 2 family ( NR2A NR2D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , the protein expression levels of several ionotropic glutamate receptor subunits ( N methyl D aspartate ( NMDA ) subunits NR 1 , NR2A , NR2B , and alpha amino 3 hydroxy 5 methyl 4 isoxazoleproprionic acid ( AMPA ) receptor subunits GluR 1 , GluR 2 , GluR 4 ) were quantified in particulate preparations from rat spinal cord at various ages after birth . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of the N methyl d aspartate receptor 1 ( NR 1 ) subunit of glutamate receptors correlated with the activity of the Sp 1 related factor , specifically through an Sp 1 element in the NR 1 promoter . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of mRNA for glutamate receptor subunits was either unaffected ( NR 1 ) or decreased ( GluR 2 and GluR 3 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The constitutive subunit of the NMDA ionotropic glutamate receptor , NMDAR 1 , was expressed in all neurons of the grey matter and was not modified in the spinal cord of mnd mice in either its normal or phosphorylated form . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We investigated the distribution of the glutamate receptor subunits , alpha amino 3 hydroxy 5 methyl 4 isoazolepropionic acid ( AMPA ) GluR 2 and GluR2 / R3 , and N methyl D aspartate ( NMDA ) NR 1 , and the timing of their appearance during early development of the mouse vestibular and spiral ganglia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Systematic mutation analysis of the human glutamate receptor , ionotropic , N methyl D aspartate 1 gene ( GRIN 1 ) in schizophrenic patients . ^^^ Mohn recently reported that transgenic mice with the reduced glutamate receptor , ionotropic , methyl D aspartate 1 gene ( GRIN 1 ) ( formerly referred to as NMDAR 1 ) expression display schizophrenia like behaviors , which can be ameliorated by antipsychotic drug treatment . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To determine if this effect was dependent on modulation of glutamate receptor gene expression , we studied the effect of gonadal hormones on levels of the NR 1 subunit of NMDA receptor and the GluR 2 subunit of the AMPA subtype of glutamate receptor in the rat striatum , using autoradiographic immunocytochemistry . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Pentamidine inhibited a constitutively active NR 1 ( T648A ) / NR2B NMDA receptor in a manner similar to its inhibition of a glutamate gated wild type NMDA receptor , but different from its inhibition of constitutively active delta 2 ( A654T ) receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have previously shown that primary human megakaryocytes express the N methyl d aspartate acid ( NMDA ) receptor 1 ( NR 1 ) subunit of NMDA type glutamate receptors , which appear to be pharmacologically similar to those identified at neuronal synapses , responsible for mediating excitatory neurotransmission in the central nervous system . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
One of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid ( AMPA ) receptors , Glutamate receptor 2 ( GluR 2 ) was also investigated to compare with NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using semi quantitative Western blot techniques and immunohistochemistry with the use of antibodies that recognize subunits of the KA ( GluR 5 , 6 , 7 ) , AMPA ( GluR 1 , GluR 2 , and GluR 4 ) , and NMDA ( NMDAR 1 and NMDAR2A / 2B ) receptors , we found no significant strain dependent differences in the expression or distribution of these glutamate receptor subunits in the intact hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate is a major excitatory neurotransmitter in the mammalian central nervous system , and the N methyl D aspartate selective glutamate receptor ( NR ) consisting of the NR 1 subunit and an NR 2 or NR 3 subunit plays crucial roles in synaptic transmission , plasticity , and learning and memory . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Further studies concentrated on the presence and the distribution of glutamate receptors : NMDAR 1 , GluR 1 , GluR 2 / 3 , GluR 5 / 6 / 7 , mGluR 2 / 3 , and mGluR 5 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Based on homology modeling to the crystal structure of the glutamate binding site of the 2 amino 3 ( 3 hydroxy 5 methyl 4 isoxazolyl ) propionic acid receptor GluR 2 , cysteines were introduced into the NR 1 subunit as chemical sensors for three thiol reactive derivatives of the competitive antagonist L 701324 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution of NADPH diaphorase and expression of nNOS , N methyl D aspartate receptor ( NMDAR 1 ) and non NMDA glutamate receptor ( GlutR 2 ) genes in the neurons of the hippocampus after domoic acid induced lesions in adult rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Functional N methyl d aspartate ( NMDA ) glutamate receptors are composed of heteromeric complexes of NR 1 , the obligatory subunit for channel activity , and NR 2 or NR 3 family members , which confer variability in the properties of the receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Organotypic hippocampal slice cultures , derived from 1 week old rats , were treated with 100 ng / ml GDNF for either 2 or 5 days , followed by Western blot analysis of NMDAr subunit 1 ( NR 1 ) and two glutamate transporter subtypes , GLAST and GLT 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Tat or NMDA treatment of mixed cultures for 24 h resulted in increased extracellular glutamate ( [ Glu ] e ) and NMDA receptor 1 ( NMDAR 1 ) expression , potential contributors to apoptosis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The glycine binding site of this subtype of ionotropic glutamate receptors is formed by the S 1 and S 2 regions of the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Tissues from the accumbens and ventral tegmental area ( VTA ) were assayed for candidate molecular neuroadaptations , including enzyme activities of cAMP dependent protein kinase ( PKA ) and adenylate cyclase ( AC ) , and protein expression of cyclin dependent kinase 5 ( cdk 5 ) , tyrosine hydroxylase ( TH ) and glutamate receptor subunits ( GluR 1 , GluR 2 and NMDAR 1 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors require both glycine and glutamate for activation with NR 1 and NR 2 forming glycine and glutamate sites , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Although the lateral dendrites of mitral and tufted cells have been reported to respond to glutamate , they did not display significant plasma membrane labeling for the NR 1 subunit or for PSD 95 , suggesting that the physiological effects of glutamate at these sites are mediated by NMDA autoreceptors that are not clustered and occur only at a low density on the dendritic surface . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA receptors are composed of a common glutamate receptor , an ionotropic NMDA 1 ( GRIN 1 ) subunit and one of four GRIN 2 subunits ( GRIN2A GRIN2D ) , combined in an undetermined ratio to make up the receptor complex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Four days later , rats were perfused and brain stem sections containing the SSN were processed for fluorescent immunohistochemistry for N methyl D aspartate receptor subunit 1 ( NMDAR 1 ) and vesicular glutamate transporters ( VGLUT 1 and VGLUT 2 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In order to explore the effects of clozapine on the NMDA class of glutamate receptors , oocytes expressing the NMDA receptor NR 1 subunit , coupled with different NR 2 subunits , were exposed to clozapine and NMDA induced current was measured with two electrode voltage clamp techniques . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The N methyl D aspartate glutamate receptor ( NMDAR ) is assembled as a tetramer containing NR 1 and NR 2 , and possibly NR 3 subunits , NR 1 being essential for the formation of the ion channel . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To understand the genetic mechanism controlling the expression of the NMDA subtype of glutamate receptors during neuronal differentiation , we studied activation of the N methyl D aspartate receptor subunit 1 ( NR 1 ) gene and the role of the repressor element 1 ( RE 1 ) element in NR 1 promoter activation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Gene expression of glutamate receptors GluR 1 and NR 1 is differentially modulated in striatal neurons in rats after 6 hydroxydopamine lesion . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We show evidence by confocal microscopy for the presence of the NR 1 subunit of the NMDA glutamate receptor subtype , proposed to be a key regulatory element , at these large endings . ^^^ Furthermore , we also show evidence by FRIL double immunogold labeling that the NR 1 subunit of the NMDA glutamate receptor is present at postsynaptic densities closely associated with gap junction plaques containing Cx 35 at mixed synapses across the goldfish hindbrain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glycine acts as a necessary coagonist for glutamate at the NMDA receptor ( NMDAR ) complex by binding to the strychnine insensitive glycine B binding site on the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
However , co expression with other related ionotropic glutamate receptor subunits ( GluR 1 and NR 1 ) does not facilitate the cell surface expression of KA 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Gene expression of NR 1 receptor subunit of N methyl D aspartate ( NMDA ) glutamate receptor in the ventral tegmental area ( VTA ) was found to be lower in anhedonic rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA receptor subunit 1 ( NR 1 ) gene is indispensable for receptor function , and knock out mice die at birth , inhibiting the study of glutamate signalling in postnatal neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Through a negative feedback loop , CaM inhibits Ca2+ influx through N methyl D aspartate type glutamate receptors in neurons by binding to the C 0 region in the cytosolic tail of the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In addition , dorsocaudal brain stem tissue lysates were harvested at 1 h , 6 h , 1 day , 3 days , 7 days , 14 days , and 30 days of SH and IH and analyzed for NR 1 , NR2A , and NR2B NMDA glutamate receptor expression by immunoblotting . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
PAT 2 is expressed in neurons positive for the N methyl d aspartate subtype glutamate receptor subunit NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In comparison with age matched control rats , a correlated decreasing expression of nitric oxide synthase ( NOS ) , glutamate and NMDAR 1 was observed in the Purkinje cells of lobules 6 8 6 h after the treatment . ^^^ Moreover , at 24 h after cisPt , the expression of glutamate , NMDAR 1 and nicotinamide adenine dinucleotide phosphate diaphorase ( NADPHd ) reactivity was further decreased . ^^^ Findings show the importance of NO glutamate interactions via NMDAR 1 in the crucial phases of Purkinje cells differentiation and their involvement on Purkinje neurons dendrite branching as demonstrated by the patterns of the other glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We studied the influence of acute and repeated morphine ( 20 mg / kg i . p . or increasing dose for 10 days ) and cocaine ( 3x20 mg / kg i . p . per day at hourly intervals , for 1 or 5 days ) administration on the expression of glutamate NMDA receptor subunit 1 ( NMDAR 1 ) in the central and basolateral nuclei of the rat amygdala and hippocampal formation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This pathway is distinguished by specific affinities of its neurons to antisera raised against glutamate , aspartate , gamma aminobutyric acid ( GABA ) , choline acetyltransferase ( ChAT ) , and a N methyl D aspartate type 1 receptor protein ( NMDAR 1 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In addition , reverse transcription polymerase chain reaction technique and sequencing analysis revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 , and KA 2 ) existed in mouse sperm as well as in human sperm . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Accordingly , we also found evidence by confocal and FRIL double immunogold labeling that the NR 1 subunit of the NMDA glutamate receptor , proposed to be a key regulatory element , is present at postsynaptic densities closely associated with gap junction plaques containing Cx 35 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Abnormal molecules included measures of synaptic density or neuronal plasticity ( reelin , SNAP 25 , BDNF , Complexin 1 and 2 ) , as well as parvalbumin , tyrosine receptor kinase A , glucocorticoid receptors , glutamate NR 1 receptor subunits , serotonin 5HT2 ( A ) and 5HT1 ( B ) receptors , and dopamine D ( 5 ) receptors . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N methyl D aspartate ( NMDA ) receptors , a subtype of glutamate receptors ( GluRs ) formed by assembly of the GluRzeta subunit ( called NR 1 in rats ) with any one of four GluRepsilon subunits ( GluRepsilon 1 4 ; NR2A D ) , play an important role in excitatory neurotransmission , synaptic plasticity and brain development . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Double immunofluorescence was performed to visualize neurons showing coexpression of DARPP 32 with NMDA or AMPA receptor subunits ( i . e . , NR 1 , NR2a / b , glutamate receptor subunit 1 [ GluR 1 ] , GluR2 / 3 , and GluR 4 ) in the forebrains of rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Molecular models of the ligand binding domain of N methyl d aspartate subunit R 1 ( NR 1 ) were made using the published crystal structures of rat glutamate receptor B ( GluRB ) , the bacterial glutamate receptor ( GluR 0 ) , and the glutamine binding protein ( QBP ) of Escherichia coli . ^^^ NR 1 prefers smaller ligands ( glycine , serine , and alanine ) in comparison with GluRB and GluR 0 that bind l glutamate : the bulky side chain of W 731 in NR 1 dramatically reduces the size of the ligand binding site , functioning to selectively restrict recognition to glycine and the d isomers of serine and alanine . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using standard immunohistochemical procedures , we investigated the changes in the expression of ionotropic glutamate receptor ( GluR ) subunits , GluRl , GluR5 / 6 / 7 , and NMDAR 1 , in the subthalamic nucleus of developing rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Our results show that : ( 1 ) retinotopic maps reorganize with time after an ischemic lesion ; ( 2 ) MAP 2 levels increase gradually from 1wPL to 5wPL ; ( 3 ) MAP 2 upregulation is associated with an increase in dendritic like structures surrounding the lesion and a decrease in GFAP positive cells ; ( 4 ) GAP 43 levels reach the highest point at 2wPL ; ( 5 ) NMDAR 1 and glutamate contents increase in parallel from 1wPL to 5wPL ; ( 6 ) GABA ( A ) alpha 1 levels increase from 1wPL to 2wPL but do not change after this time point ; and ( 7 ) GABA contents remain low from 1wPL to 5wPL . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In addition , RT PCR results revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 and KA 2 ) were expressed in mouse testis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Moreover , activation of metabotropic glutamate receptors leads to the recruitment of the NMDA receptor subunit NR 1 to remodeled ER export sites . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Chronic PCP treated animals showed significant alterations in glutamate receptor subunits , particularly for the NR 1 , NR2B , NR2C , and NR2D components of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Studies of p 38 mitogen activated protein kinase ( MAPK ) activation , morphologic changes including cell number , and the expression of the NR 1 and GluR 2 subunits , by reverse transcriptase PCR were evaluated at early postnatal ages ( postnatal day [ PD ] 8 14 ) in cerebral cortex of rats treated with monosodium glutamate ( MSG ; 4 mg / g body weight ) administered subcutaneously on PD 1 , 3 , 5 , and 7 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In goldfish brain , confocal microscopy revealed immunofluorescence for connexin 35 ( Cx 35 ) and NMDA R 1 ( NR 1 ) glutamate receptor protein in Mauthner Cell / Club Ending synapses . ^^^ By FRIL double labeling , NR 1 glutamate receptors were found in clusters of intramembrane particles in the postsynaptic membrane extraplasmic leaflets , and these distinctive postsynaptic densities were in close proximity ( 0 . 1 0 . 3 microm ) to neuronal gap junctions labeled for Cx 35 , which is the fish ortholog of connexin 36 ( Cx 36 ) found at neuronal gap junctions in mammals . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The possible implications of molecular mechanism underlying the neurotoxicity in association with necrosis , apoptosis , nitric oxide synthases ( nNos and iNOS ) and glutamate receptors ( NMDAR 1 and GluR 2 ) related genes and their expression in DA induced neuronal damage in the hippocampus have been discussed . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using a second approach in vitro , we confirmed that both exogenous glutamate and BDNF ( but not other neurotrophins ) were able to induce NR 1 phosphorylation , in particular at residue Ser 897 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamatergic sites were localized with vesicular glutamate transporter type 1 ( VGLUT 1 ) as presynaptic marker and the NR 1 subunit of the NMDA receptor and the GluR 1 subunit of the AMPA receptor as receptor markers . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The increased levels of extracellular glutamate , NMDA receptor zeta subunit ( NR 1 ) mRNA , NMDA receptor epsilon 1 subunit ( NR2A ) protein , phosphorylated Ca ( 2+ ) / calmodulin kinase 2 ( p CaMKII ) protein , c fos mRNA , c Fos protein , are observed in the specific brain areas of mice and / or rats showing signs of naloxone precipitated withdrawal . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RT PCR demonstrated that ionotropic ( iGluR NR 1 ) and metabotropic ( mGluR Group 3 ) glutamate receptors are expressed in rodent lymphocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Under standard conditions , cultured ventral spinal neurons cluster AMPA but not NMDA type glutamate receptors at excitatory synapses on their dendritic shafts in spite of abundant expression of the ubiquitous NMDA receptor subunit NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glycine coactivates glutamate N methyl D aspartate ( NMDA ) receptors by binding to a distinct recognition site on the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , we employ the glutamate NMDA ( N methyl D aspartate ) R 1 receptor ( GRIN 1 ) transcript as a model system to identify the molecular determinants for a brain region specific exon silencing mechanism . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study examined the expression of glutamate , N methyl D aspartate receptor subunit 1 ( NMDAR 1 ) , GABA , GABAB receptors , glycine receptors , and glutamate receptor subunit 2 ( GluR 2 ) in the ventrolateral subnucleus of the solitary tract nucleus , nucleus ambiguus , hypoglossal nucleus , medial accessory olivary nucleus , dorsal motor nucleus of the vagus , and cuneate nucleus , from P 2 to P 21 in rats . ^^^ Results showed that 1 ) the expression of glutamate increased with age in a majority of the nuclei , whereas that of NMDAR 1 showed heterogeneity among the nuclei ; 2 ) GABA and GABAB expressions decreased with age , whereas that of glycine receptors increased with age ; 3 ) GluR 2 showed two peaks , at P 3 4 and P 12 ; and 4 ) glutamate and NMDAR 1 showed a significant reduction , whereas GABA , GABAB receptors , glycine receptors , and GluR 2 exhibited a concomitant increase at P 12 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used antibodies against the following proteins and amino acids : the neurotransmitters glutamate and gamma aminobutyric acid ( GABA ) ; the GABA synthesizing enzyme glutamic acid decarboxylase ( GAD ) ; GABA transporter 1 ; the anchoring protein for GABA and glycine receptors , gephyrin ; the calcium binding proteins calbindin and calretinin ; the NR 1 subunit of the N methyl D aspartate glutamate receptor ; the metabotropic glutamate receptors mGluR1alpha , mGluR2 / 3 , and mGluR 5 ; and the intracellular signaling molecules calcineurin , calcium calmodulin kinase IIalpha ( CAMKIIalpha ) and the receptor for inositol triphosphate ( IP3R1alpha ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The resolved 10 ray crystal structures of the glutamate binding domain ( S1 / S2 domains ) of the GluR 2 and NR 1 glutamate receptor subunits were used to model the homologous regions of the N methyl D aspartate ( NMDA ) receptor ' s NR 2 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA subtype of glutamate receptors are heteromeric complexes comprised of multiple subunits encoded by at least seven different genes ( NR 1 , NR2A 2D and NR3A 3B ) , and differential expression of these subunits alters the pharmacological and electrophysiological properties of NMDA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we use molecular approaches to identify a ternary combination of exonic UAGG and 5 ' splice site proximal GGGG motifs that functions cooperatively to silence the brain region specific CI cassette exon ( exon 19 ) of the glutamate NMDA R 1 receptor ( GRIN 1 ) transcript . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Given that glutamate and its interaction with dopamine regulate motivational and associative processing in this brain region , we assessed expression of the NR 1 subunit of the N methyl D aspartate ( NMDA ) receptor and the GluR 1 subunit of the alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) receptor in the NAcc core and shell of EE and SI rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Real time RT PCR using TaqMan probes was employed to quantify mRNA for proteolipid protein , glyceraldehyde 3 phosphate dehydrogenase , glial fibrillary acidic protein , nestin , and the GluR 2 and NR 1 subunits of glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At 30 days after CFA injection , effects of EA on ionotropic glutamate receptor ( NR 1 , NR 2A , GluR 1 and GluR 2 / 3 ) expression in association with c fos and calcitonin gene related peptide ( CGRP ) expression were observed in the dorsal horn of the spinal cord using immunohistochemistry . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Synapses at P 2 contained substantial levels of NR 1 and NR2B and most GluR associated proteins , including SAP 102 , SynGAP , the chain of proteins from GluRs / SAP102 through GKAP / Shank / Homer and metabotropic glutamate receptors , and the adhesion factors , cadherin , catenin , neuroligin , and Nr CAM . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The antagonist bound conformation of the NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) ionotropic glutamate receptor are modeled using the crystal structure of the DCKA ( 5 , 7 dichloro kynurenic acid ) bound form of the NR 1 subunit ligand binding core ( S1S2 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To test whether the induction of multiple dopamine signaling molecules in the lateral ganglionic eminence was due to a general enhancement of neuronal differentiation by retinoic acid , we assayed the effects of retinoic acid on other differentiation markers , including glutamate decarboxylase 65 , NR 1 subunit of glutamate NMDA receptor and microtubule associated protein 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The N methyl D aspartate family of glutamate receptors ( NMDARs ) are tetrameric cation channels including NR 1 , NR 2 , and possibly NR 3 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study examined whether the hypothalamic expression of three key ionotropic glutamate receptor subunits ( NMDAR 1 , GluR 1 and GluR 6 ) fluctuates significantly on proestrus in the rat , and whether treatment with the antiprogestin , RU 486 affected glutamate receptor subunit expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the absence of exogenous glutamate and glycine , the rate of association of [ 3H ] MK 801 with wild type NR 1 1a / NR2A receptors was considerably slower than that for [ 3H ] dextrorphan . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using a native chromatin immunoprecipitation assay , we studied histone methylation marks at proximal promoters of 16 ionotropic and metabotropic glutamate receptor genes ( GRIN 1 , 2A D ; GRIA 1 , 3 , 4 ; GRIK 2 , 4 , 5 ; GRM 1 , 3 , 4 , 6 , 7 ) in cerebellar cortex collected across a wide age range from midgestation to 90 years old . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 protein expression in cultured cortical neurons was specifically reduced by stimulation with 100 microM NMDA or glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) type glutamate ( GluR2 / 3 ) receptors and N methyl D aspartate receptor subtype 1 ( NMDAR 1 ) was carried out by immunohistochemistry , double immunofluorescence and real time RT PCR analysis in the pineal glands of 1 day to 6 wk old rats in the present study . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
They are heteromeric complexes of NR 1 combined with NR2A D and / or NR3A B subunits that are activated by glutamate and glycine and whose activity is modulated by allosteric modulators . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Post anoxic rats showed a reduction in the expression of group 1 metabotropic glutamate receptors ( i . e . mGlu 1 and mGlu 5 receptors ) in the hippocampus and cerebral cortex , whereas expression of the mGlu 2 / 3 receptors , the NR 1 subunit of NMDA receptors , and the GluR 1 subunit of alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( AMPA ) receptors was unchanged . mGlu 1 and mGlu 5 receptor signalling was also impaired in postanoxic rats , as revealed by a reduced efficacy of the agonist ( 1S , 3R ) 1 Aminocyclopentane 1 , 3 dicarboxylic acid ( 1S , 3R ACPD ) to stimulate polyphosphoinositide hydrolysis in hippocampal slices . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We investigated in the rat the effect of axotomy on the different subunits of excitatory glutamatergic AMPA ( GLuR 1 4 ) , NMDA ( NR 1 , NR2A D ) receptors , post synaptic density 95 , vesicular glutamate transporter 2 , beta catenin and cadherin . mRNA levels and / or protein production were analyzed 1 , 3 , 8 , 30 and 60 days after facial nerve axotomy by in situ hybridization and immunohistofluorescence . mRNAs coding for the GLuR 2 4 , NR 1 , NR2A , B , D subunits of glutamatergic receptors and for post synaptic density 95 , were less abundant after axotomy . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The labeling density for epsilon 2 but not zeta 1 ( NR 1 ) and subtype 2 / 3 glutamate receptor ( GluR2 / 3 ) in Sch CA 1 pyramidal cell synapses was significantly different between the left and right hippocampus with opposite directions in strata oriens and radiatum ; the left to right ratio of epsilon 2 labeling density was 1 : 1 . 50 in stratum oriens and 1 . 44 : 1 in stratum radiatum . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This unique type of glutamate receptor was reported to be activated by the traditional agonists of all three major glutamate receptor subfamilies [ i . e . , alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate ( AMPA ) , kainate , and N methyl d aspartate ( NMDA ) ] in a glycine dependent as well as magnesium blockable manner and was reported to consist of an NR 1 subunit coexpressed with the kainate binding protein ( KBP ) from Xenopus laevis , XenU 1 . ^^^ Because we verified that XenU 1 is expressed and inserted into the plasma membrane of HEK 293 cells , we conclude that XenU 1 and NR 1 do not form the postulated unitary glutamate receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We performed immunofluorescent double labeling for the NR 1 subunit of the N methyl D aspartate receptor ( NMDAR ) and the vesicular glutamate transporter 1 ( VGLUT 1 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We show that responses induced by 1mM glutamate in NR 1 1a / NR2A and NR 1 1a / NR2B receptors are potentiated five to eight fold more by pregnenolone sulfate than responses of NR 1 1a / NR2C and NR 1 1a / NR2D receptors with no differences in the concentration of pregnenolone sulfate that produced 50 % potentiation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These receptors are heteromeric ion channels that for activation require binding of glycine and glutamate to the NR 1 and NR 2 subunits , respectively . ^^^ Here we report crystal structures of the ligand binding core of NR2A with glutamate and that of the NR 1 NR2A heterodimer with glutamate and glycine . ^^^ The NR2A glutamate complex defines the determinants of glutamate and NMDA recognition , and the NR 1 NR2A heterodimer suggests a mechanism for ligand induced ion channel opening . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Regulation of phosphorylation of NMDA receptor NR 1 subunits in the rat neostriatum by group 1 metabotropic glutamate receptors in vivo . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Developmental gap junction uncoupling and Cx 36 downregulation are not affected by blockade of non NMDA glutamate receptors , and do not occur in hypothalamic neurons from NMDA receptor subunit 1 ( NMDAR 1 ) knockout mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
While there were no alterations in the glutamate sensitivity , channel open probability or the single channel conductance of the receptor , a crosslinking assay demonstrated that the NR 1 and NR2A subunits of NMDA receptors were preferentially retained in the cytoplasm after SD , indicating that SD alters NMDAR surface expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate exhibited a marginally higher apparent affinity for NR2A containing receptors than NR2B containing receptors , regardless of the NR 1 subunit present . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast , mutant myosin Vb did not change the localization of several other neurotransmitter receptors , including the glutamate receptor subunit NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Sections were processed and analyzed for [ 3H ] glutamate binding to NMDA receptors using receptor autoradiography and for mRNA for the zeta 1 ( NR 1 ) , epsilon 1 ( NR2A ) , and epsilon 2 ( NR2B ) subunits of the NMDA receptor using in situ hybridization . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Identification and localisation of the NR 1 sub unit homologue of the NMDA glutamate receptor in the honeybee brain . ^^^ The NR 1 sub unit homologue of the NMDA glutamate receptor was characterised in the honeybee . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The mRNA and protein expression of endothelial , neuronal , and inducible nitric oxide synthase ( eNOS , nNOS , and iNOS , respectively ) , hypoxia inducible factor 1alpha ( HIF 1alpha ) , vascular endothelial growth factor ( VEGF ) , N methyl D aspartate receptor subunit 1 ( NMDAR 1 ) , and alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid glutamate ( AMPA GluR 2 and GluR 3 ) receptors in the retina was determined by real time RT PCR , Western blot analysis , and immunohistochemistry . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
GT 1 7 neurons express mRNAs for various mouse NMDA receptor subunits ( zeta , 1 , epsilon 3 , epsilon 4 , and epsilon 2 , corresponding to the NR 1 , NR2C , NR2D , and NR2B rat subunits ) and increase their spontaneous release of GnRH when incubated in the presence of exogenous glutamate or NMDA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Neuronal degeneration was paralleled by reduced glutamate receptor ( NR 1 , GluR 1 and GluR6 / 7 ) immunolabeling throughout the whole hippocampal formation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Cellular studies reveal that the ionotropic glutamate receptors , N methyl D aspartate receptors ( NMDARs ) , can be phosphorylated on their NR 1 subunits at the C terminal serine residues , which is a major mechanism for the regulation of NMDAR functions . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate , which binds to NR 2 subunits , also binds to NR3A , but with very low affinity ( Kd = 9 . 6 mm ) ; in contrast , binding of glutamate to NR 1 was not detectable even at a 300 mm concentration . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Although Con G is known to bind to the glutamate binding site on the NR 2 subunit of the receptor , it is unclear whether it can allosterically modulate the function of the receptor through the glycine binding site on the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Applying semi quantitative immunoblotting , we found that in the whole tetanized hippocampus , synaptic expression of the N methyl D aspartate and alpha amino 3 hydroxyl 5 methyl 4 isoxazole propionate receptor subunits ( NR 1 , NR2A , glutamate receptor 1 ) and their associated partners , e . g . synaptic associated protein 97 , postsynaptic density protein 95 , alpha subunit of Ca2+ / calmodulin dependent protein kinase 2 , neuronal nitricoxide synthase , increased 180 min post high frequency stimulation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Phylogenetic analysis suggests that both the AcNR1s and LsNR1s belong to the NR 1 subgroup of ionotrophic glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using double immunocytochemistry , we found that rats exhibiting panic like responses ( e . g . , L AG plus lactate ) had increased c Fos immunoreactivity in DMH neurons expressing the NMDA receptor 1 ( NR 1 ) subunit , but not those expressing the glutamate receptor 2 and 3 subunits of the AMPA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These studies indeed showed that in EGC cultures , mRNA of the glutamate receptor subunits NR 1 , NR2A / B , GluR 1 , GluR 3 , and GluR 5 and the protein bands of the glutamate receptor subunits NR2A / B , GluR 1 , GluR 3 , and GluR 5 could be detected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Detection of the phosphorylation of cAMP response element binding protein ( pCREB ) revealed that a subset of NR 1 positive hNPC could respond to glutamate . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N Methyl D aspartate receptor subunit genes ( gene symbol , GRIN ) GRIN 1 , GRIN2A , GRIN2B , GRIN2C , and GRIN2D have been assigned to chromosomes ( Chr ) 3 , 10 , 4 , 10 , and 1 , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate receptor , ionotropic , N methyl D aspartate 2A ( GRIN2A ) gene as a positional candidate for attention deficit / hyperactivity disorder in the 16p13 region . ^^^ The glutamate receptor , ionotropic , N methyl D aspartate 2A ( GRIN2A ) gene that encodes the 2A subunit of the NMDA receptor , resides in this region and a recent study has reported an association between this gene and ADHD . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The GRIN2A ( glutamate receptor , ionotropic , N methyl D aspartate 2A ) gene that encodes the N methyl D aspartate receptor subunit 2A ( NMDA2A ) maps to this region of linkage . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
There is also growing evidence for the role of DRD 1 , NMDA receptor genes ( GRIN 1 , GRIN2A , GRIN2B ) , brain derived neurotrophic factor ( BDNF ) , and dopamine transporter ( SLC6A3 ) in both schizophrenia and bipolar disorder . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Two NMDA receptor subunit genes , GRIN 1 and GRIN2A , are both good candidate genes for schizophrenia . ^^^ METHOD : We genotyped five single nucleotide polymorphisms ( SNPs ) in GRIN 1 and two in GRIN2A in 2455 Han Chinese subjects , including population and family based samples , and performed case control and transmission disequilibrium test ( TDT ) analyses . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Optimal expression of cloned NMDAR1 / NMDAR2A heteromeric glutamate receptors : a biochemical characterization . ^^^ The N methyl D aspartate R 1 ( NMDAR 1 ) and NMDAR2A subunits were expressed transiently either alone or in combination in human embryonic kidney ( HEK ) 293 cells . ^^^ Co expression of NMDAR 1 and NMDAR2A subunits in HEK 293 cells resulted in cell death . ^^^ These results demonstrate that the NMDAR 1 and NMDAR2A receptor subunits co assemble to form a heteromeric complex with properties similar to those of the native receptors of adult mammalian forebrain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors are believed to be heterooligomers of subunits from two families : NMDAR 1 , encoded by a single gene but alternatively spliced to produce eight distinct isoforms ( NMDAR1A H ) , and NMDAR 2 , encoded by four separate genes ( NMDAR2A D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDAR 1 possesses all properties characteristic of the NMDA receptor channel complex , whereas the four NMDAR 2 subunits , termed NMDAR2A 2D , show no channel activity but potentiate the NMDAR 1 activity and confer functional variability by different heteromeric formations . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Several subunits of the NMDA receptor have been cloned : these are NMDAR 1 ( NR 1 ) , and NMDAR2A , 2B , 2C and 2D ( NR2A D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This receptor is composed of a fundamental subunit ( NMDAR 1 ) and its potentiating subunits ( NMDAR2A NMDAR2D ) . ^^^ We constructed a series of mutations with single amino acid substitutions in the second transmembrane segment of NMDAR 1 and examined channel properties of the resultant mutants in combined expression with the NMDAR2A subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The laminar distribution and cellular levels of expression of mRNAs encoding N methyl D aspartate receptor subunits ( NMDAR 1 , NMDAR2A D and the alternatively spliced isoforms of NMDAR 1 ) were examined in prefrontal cortex of rat by in situ hybridization using film and emulsion autoradiography . ^^^ Film autoradiograms demonstrated a distinctive laminar distribution of hybridization signals for each of the probes recognizing NMDAR 1 , NMDAR2A , and NMDAR2B messenger RNA ; hybridization with probes for NMDAR2C and NMDAR2D resulted in scattered signals without laminar organization . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of the N methyl D aspartate ( NMDA ) receptor subunit mRNAs NMDAR 1 and NMDAR2A D was characterized in undifferentiated and nerve growth factor ( NGF ) differentiated PC 12 cells using Northern blotting , RNase protection assays ( RPA ) and polymerase chain reaction ( PCR ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Purkinje cells showed very dense labeling for NMDAR 1 mRNA , dense labeling for NMDAR2A mRNA , and moderate labeling for NMDAR2D mRNA , whereas labeling for NMDAR2C mRNA was low . ^^^ Granule cells showed high hybridization signals for the NMDAR 1 and NMDAR2C mRNAs and moderate signals for the NMDAR2A and NMDAR2D mRNAs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Dual in situ hybridization using digoxigenin labeled GnRH cRNA probes and 35S labeled glutamate receptor subunit probes , followed by autoradiography and image analysis were used to measure the KA 2 or NMDAR2A mRNA content in GnRH neurons in 20 to 50 day old female rats which were sacrificed at 08 . 00 or 17 . 00 h . ^^^ We have previously shown that , in the female rat , GnRH neurons preferentially express the KA 2 and NMDAR2A receptor subunit mRNAs , but not AMPA or NMDAR 1 mRNA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Acute heat stress induces edema and nitric oxide synthase upregulation and down regulates mRNA levels of the NMDAR 1 , NMDAR2A and NMDAR2B subunits in the rat hippocampus . ^^^ On the other hand NMDA receptor messenger RNA encoding NMDAR 1 , NMDAR2A and NMDAR2B subunits showed a marked downregulation in the hippocampus of heat stressed rats compared to the controls . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Heavy labeling for NMDAR 1 subunit mRNA was observed in all major CN neuronal types with lower labeling for NMDAR2A , 2B , 2C , and 2D mRNA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Frozen sections and serial plastic sections of tissue were incubated with antibodies to NMDAR 1 ( NR 1 ) , NMDAR2A ( NR2A ) , NMDAR2A / B ( NR2A / B ) , and NMDAR2B ( NR2B ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The effects of prenatal ethanol exposure on the NMDAR 1 protein expression ( postnatal days 1 and 7 ) and on the developmental profile of the NMDAR2A and NMDAR2B subunits in rat forebrain and hippocampus were investigated . ^^^ A semiquantitative immunoblot procedure was used with antibodies raised against the NMDAR 1 , NMDAR2A , and the NMDAR2B subunits to assess the NMDA subunit protein expression in the samples . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used quantitative double label in situ hybridization to examine in rat brain the expression of NMDAR 1 , NMDAR2A , NMDAR2B , and NMDAR2C mRNA in six neurochemically defined populations of striatal neurons : preproenkephalin ( ENK ) and preprotachykinin ( SP ) expressing projection neurons , and somatostatin ( SOM ) , glutamic acid decarboxylase 67 ( GAD 67 ) , parvalbumin ( PARV ) , and choline acetyltransferase ( ChAT ) expressing interneurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , the cellular expression of the NMDAR 1 ( NR 1 ) and NMDAR2A and B ( NR2A and B ) subunits was investigated in the human cerebral cortex by immunocytochemistry with antibodies that recognize the NR 1 or the NR2A and B subunits of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N methyl D aspartate ( NMDA ) receptor 1 ( NMDAR 1 ) , NMDAR2A , and NMDAR2B mRNAs were expressed highly throughout the PVN and its perinuclear region as well as in the subparaventricular zone . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blot analysis also failed to reveal any changes in NMDAR 1 , NMDAR2A , or NMDAR2B receptor levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This approach has allowed us to assess the responses of individual NTS neurones in vivo to ionotropic glutamate receptor agonists and antagonists and then , to determine whether the neurones expressed the glutamate receptor subunits , GLUR 2 , 3 and NMDAR2a , b . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The mRNA expression of the subunits of rat hippocampus NMDAR NMDAR 1 , NMDAR2A , NMDAR2B , NMDAR2D was determined with RT PCR technique in C group and at 0 . 5 , 1 , 2 and 4 postburn hours ( PBHs ) in S group . ^^^ But the mRNA expression of NMDAR 1 increased for 24 . 3 % and 20 . 9 % and that of NMDAR2A increased for 27 . 8 % and 27 . 6 % at 2 and 4 PBHs respectively when compared with that in C group ( P < 0 . 05 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This , together with changing subunit composition during the different stages ( increasing amounts of NMDAR 1 subunits and appearance of NMDAR2A subunits in adults ) , suggests that the amount of functional NMDA receptors needed to achieve a fully developed reflex is not attained until later stages . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Furthermore , in HEK 293 cells co transfected with NR2A and NR 1 subunits containing the C terminal exon with the PKC phosphorylation sites , our preliminary studies indicate that the NMDA evoked current is potentiated by intracellular PKC . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate activated single channel and ensemble currents were recorded from Xenopus laevis oocytes and HEK 293 cells expressing a recombinant NMDA receptor , assembled from NR 1 and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoaffinity chromatography of detergent extracts of rat synaptic plasma membranes on anti phosphotyrosine antibody agarose showed that the NR2A and NR2B subunits but not the NR 1 subunit are tyrosine phosphorylated . ^^^ Conversely , immunoprecipitation of the NR 1 , NR2A , and NR2B subunits with subunit specific antibodies followed by immunoblotting with anti phosphotyrosine antibodies confirmed that the NR2A and NR2B subunits but not the NR 1 subunit were phosphorylated on tyrosine residues . ^^^ In addition , endogenous protein tyrosine kinases in synaptic plasma membranes phosphorylated the NR2A subunit in vitro , increasing its phosphorylation 6 8 fold but did not phosphorylate NR 1 or NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The reduction in NMDA receptor activity was accompanied by a significant decrease ( approximately 80 % at 9 days in vitro ) in the level of the NR 1 and the NR2A NMDA receptor subunit protein , detected using the selective photoaffinity ligand [ 125I ] CGP55802A . ^^^ In contrast to the marked influence of chronic NMDA exposure on the cellular content of the NMDA receptor subunit proteins , mRNA levels of the different subunits ( NR 1 , NR2A , NR2B and NR2C ) were not significantly affected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Finally , three NR 1 mutants which have been previously shown by others to possess either decreased Mg2+ sensitivity and Ca2+ permeability ( N616Q and N616R ) or reduced current amplitude ( F609L ) were tested for their ethanol sensitivity when expressed in combination with the NR2A subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Among the NMDA receptor subunits , NR 1 was most highly expressed in the SCN followed in order of abundance by NR2B , NR2A , NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Five N methyl D aspartate ( NMDA ) receptor subunits have been identified thus far : NR 1 , NR2A , NR2B , NR2C , and NR2D . ^^^ In the external granular layer , hybridization signals of mRNAs for NR 1 , NR2A , NR2B , and NR2C appeared by postnatal day 3 , but no NR2D mRNA was expressed at any developmental stage examined . ^^^ In the cerebellar nuclei , mRNAs for NR 1 , NR2A , NR2B , and NR2D were more or less expressed on postnatal day 0 , while expression signals for the NR2C mRNA were first detected in postnatal day 14 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Pronounced increases in the levels of mRNAs encoding NR 1 and NR2A were seen in the cerebral cortex , hippocampus , and cerebellum between postnatal days 7 and 20 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The correspondence between NMDA receptor activity and the amount of NR 1 and NR2A subunit proteins was excellent , the rank order being K 25 > K 10 + NMDA > K 10 at 9 days in vitro . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
By combining human NR 1 with rat NR2A and NR2C we have studied the pharmacological properties of three possible NMDA receptor subtypes ; NR 1 + NR2A , NR 1 + NR2C and NR 1 + NR2A + NR2C . ^^^ By performing glycine concentration response curves and comparing EC50s , it was possible to show that the NR 1 + NR2A + NR2C receptor preferentially co assembled when all three subunit cDNAs were present . ^^^ This receptor had an affinity for glycine intermediate between that of NR 1 + NR2A and NR 1 + NR2C , but a similar Hill coefficient . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This antibody ( NR2A / B ) did not cross react with extracts from transfected cells expressing other glutamate receptor subunits , nor did it label non neuronal tissues . ^^^ Neuronal plasticity associated with learning , memory and development is controlled , in part , by NMDA receptors , which are complexes consisting of the subunit NMDAR 1 ( NR 1 ) and one or more NMDAR 2 subunits ( NR2A NR2D ) . ^^^ The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR 1 . ^^^ In the cerebellum , in contrast to staining with NR 1 antibody , Purkinje cell staining with NR2A / B antibody was low , indicating that these neurons may lack functional NMDA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Histamine potentiated responses to NMDA at heteromeric NR1 / NR2 receptors containing splice variants of the NR 1 subunit ( NR1A or NR1E ) that lack the amino terminal insert , together with the NR2B subunit but not the NR2A or NR2C subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression patterns of nine genes encoding the N methyl D aspartate ( NMDA ) receptor subunits NR 1 and NR2A , NR2B , NR2C and NR2D , and the high affinity kainate receptor subunits KA 1 , KA 2 , GluR 6 and GluR 7 , were studied in the adult rat retina by in situ hybridization . ^^^ Hybridization with [ 35S ] dATP labelled oligonucleotide probes revealed the expression of four of the NMDA receptor subunits ( NR 1 , NR2A , NR2B and NR2C ) and three of the high affinity kainate receptor subunits ( KA 2 , GluR 6 and GluR 7 ) in the retina . ^^^ In the ganglion cell layer , virtually every ganglion cell or displaced amacrine cell expressed the receptor subunits NR 1 , NR2A , NR2B , NR2C , KA 2 and GluR 7 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 ( 011 ) , the most prominent NR 1 splice variant in rat forebrain , and NR 1 ( 100 ) , prominent in midbrain , were expressed in Xenopus oocytes singly and in combination with NR2A , NR2B , and NR2C subunits . ^^^ NR 1 ( 011 ) / NR2A receptors exhibited only the increase in glycine affinity , and NR 1 ( 011 ) / NR2C receptors exhibited neither . ^^^ As for NR 1 ( 100 ) homomers , NR 1 ( 100 ) / NR2B and NR 1 ( 100 ) / NR2A receptors exhibited spermine potentiation only by increasing the glycine affinity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
A concomitant reduction in levels of NR2B but not NR2A occurred in NR 1 / mice , demonstrating that there is an interdependence of subunit expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
As a model of complex partial seizures , rapid hippocampal kindling was chosen to investigate changes in the expression of messenger RNAs encoding the N methyl D aspartate receptor subunits NR 1 , NR2A and NR2B both during and in the period immediately following the induction of the kindled state . ^^^ In partially kindled animals ( 10 stimulations ) , while the NR 1 subunit messenger RNA remained unaltered after a period of 2 h , the NR2A and NR2B subunit messenger RNAs were bilaterally reduced in dentate gyrus granule cells by around 50 % below control values . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Cloning , functional coexpression , and pharmacological characterisation of human cDNAs encoding NMDA receptor NR 1 and NR2A subunits . ^^^ Using expression cloning , and more recently using polymerase chain reaction cloning approaches , a family of rat N methyl D aspartate ( NMDA ) receptor subunit cDNAs has been described ( NR 1 , NR2A , NR2B , NR2C , and NR2D ) . ^^^ Here we report cloning and sequencing of cDNAs encoding isoforms of the human NR 1 subunit ( NR1a , NR1d , and NR1e ) that differ at their C terminal end as a result of alternative splicing and also of a cDNA encoding the human NR2A subunit . ^^^ No modulatory effect was observed on application of ifenprodil , confirming previous observations with rat NR 1 + NR2A recombinant receptors . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At this developmental age , levels of NR 1 , NR2A , NR2B , and NR2C mRNA were low or undetectable . ^^^ The increase in the expression of receptors having a low affinity for ifenprodil and the increase in NR 1 and NR2A mRNAs were reduced or prevented by maintaining cells in medium with a low concentration of serum . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of 16 genes , encoding all known subunits for the NMDA receptor ( NR 1 , NR2A to NR2D ) , AMPA / low affinity kainate ( GluR A to D ) , high affinity kainate ionotropic receptors ( KA 1 , 2 , GluR 5 to 7 ) and two orphan receptor subunits ( delta 1 and 2 ) was examined by in situ hybridization in rat lumbar spinal cord , and in the periaqueductal gray . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
When the NR 1 subunit was coexpressed with either the NR2A , NR2B , or NR2C subunit , only the combination of NR1 / NR2A was photoreactive . ^^^ Both the NR 1 and NR2A subunits were photolabeled , corresponding in size to the proteins labeled in situ . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA treatment also elicited a decrease in NR 1 , NR2A , and NR2B subunit messenger RNA ( mRNA ) levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
When expressed together , wild type NR2A and mutant NR 1 ( N598R ) subunits produced channels with a main conductance of 2 . 6 pS and a sublevel of 1 . 2 pS . ^^^ These conductances were clearly different from those obtained from wild type NR 1 and wild type NR2A channels which gave characteristic 50 pS events with a 40 pS sublevel . ^^^ When wild type and mutant NR 1 subunits were co expressed together with NR2A subunits a different channel type with a main conductance of 15 . 2 pS and a sublevel of 11 . 4 pS was obtained , as well as the ' all wild type ' and ' all mutant ' channels described above . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Reverse transcription PCR assays were used to measure levels of NMDA receptor ( NR ) subunit mRNAs encoding splice variants of NR 1 ( NR1a , exon 5 ; NR1b , +exon 5 ) and the major NR 2 subunits ( NR2A , NR2B , and NR2C ) in dissociated cerebellar granule cell cultures . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the cerebral cortex of both schizophrenics and controls , mRNAs for NR 1 , NR2A , NR2B , and NR2D subunits were preferentially expressed in layers II / III , Va , and VIa , with much higher levels in the prefrontal than in the parieto temporal cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have made several amino acid substitutions downstream of the Q / R / N site in the TMII region of both NR 1 and NR2A subunits . ^^^ Mutant NR 1 subunits were coexpressed with wild type NR2A subunits and vice versa in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus human embryonic kidney ( HEK ) 293 cells were cotransfected with N methyl D aspartate R 1 ( NR 1 ) and N methyl D aspartate R2A ( NR2A ) clones and the cells grown post transfection in the presence of tunicamycin ( TM ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We further characterized the glutamate site assembled from NR1a , NR2A , and NR2B NMDA receptor subunits using L [ 3H ] glutamate and [ 3H ] CGP 39653 binding assays . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Ethanol treatment did not affect mRNA levels for NR 1 or NR2A , caused only a small increase ( 20 % ) in protein levels for NR 1 , and resulted in a decrease ( 30 % ) in NR2A protein . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Differential contribution of the NR 1 and NR2A subunits to the selectivity filter of recombinant NMDA receptor channels . 1 . ^^^ The molecular determinants for the narrow constriction of recombinant N methyl D aspartate ( NMDA ) receptor channels composed of wild type and mutant NR 1 and NR2A subunits were studied in Xenopus oocytes . 2 . ^^^ Mutation of the N site asparagine ( N ) to glutamine ( Q ) at homologous positions in either NR 1 ( position 598 ) or NR2A ( position 595 ) increased the permeability of DMA relative to wild type channels about equally . ^^^ However , for larger sized organic cations , the NR 1 ( N598Q ) mutation had stronger effects on increasing their permeability whereas the NR2A ( N595Q ) mutation was without effect . ^^^ These changes in organic cation permeability suggest that the NR 1 ( N598Q ) mutation increases the pore size while the NR2A ( N595Q ) mutation does not . 5 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Our current work demonstrates that chronic ethanol ingestion by mice , which results in the generation of physical dependence , also produces increases in the NMDA receptor NR 1 subunit protein in the hippocampus and cerebellum ( approximately 50 % and 95 % , respectively ) , and produces increases in the NR2A subunit protein in the hippocampus and cortex ( approximately 25 % and 40 % , respectively ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
A sensitive RNase protection assay was employed to determine simultaneously the mRNA levels of NR 1 subunit ( comprising all different splice variants ) and three NR 2 subunits ( NR2A NR2C ) . ^^^ The relative distribution profile of NMDA receptor subunits in the cerebral cortex was NR2B > NR 1 > NR2A > NR2C and in the cerebellum NR2C = NR 1 > NR2A = NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We found that more than 70 % of CRF positive neurons contain the NMDA receptor subunit NR 1 mRNA whereas NR2A and NR2B subunit mRNAs were not detectable in CRF cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Decreased cytotoxicity was observed when a mutated NR 1 subunit ( N616R ) with reduced Ca++ permeability was used in coexpression studies with NR2A or NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Similarly , the NR2D subunit is selectively and quantitatively immunoprecipitated , whereas the NR 1 , NR2A , or NR2B subunit is not . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The receptor is a heteromultimer of core subunits , NR 1 , and one or more regulatory subunits , NR2A D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This change in pharmacology coincides with a developmental switch in subunit expression ; from NR 1 expressed with NR2B to NR 1 coexpressed with NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used electron microscopic immunocytochemistry with antibodies against NR 1 and NR2A and B subunits to study the distribution of N methyl D aspartate ( NMDA ) receptors in presynaptic axon terminals in the rat cerebral cortex . ^^^ In all sections examined , NR 1 and NR2A / B immunoreactivities were observed in axon terminals : NR 1 and NR2A / B positive axon terminals made both symmetrical and asymmetrical synapses on unlabelled dendritic profiles . ^^^ Combined pre and postembedding studies showed that all NR 1 and NR2A / B positive axon terminals making symmetrical synapses were gamma aminobutyric acid ( GABA ) positive . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NR 1 subunit possesses all properties of the NMDA receptor channel complex , whereas four NR 2 subunits ( NR2A 2D ) potentiate and differentiate NMDA receptor responses by heteromeric assemblies with NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoaffinity purification of NMDA receptors from forebrain by anti NR 1 911 920 antibody affinity chromatography from 1 % Triton 10 100 / 1 M NaCl solubilised extracts yielded purification of the NR 1 Mr 120 , 000 immunoreactive species , but no detectable NR2A or NR2B immunoreactivity . ^^^ Immunoprecipitation of NMDA receptors from Triton 10 100 / 1 M NaCl extracts with anti NR 1 911 920 antibodies also resulted in precipitation of NR 1 subunits , but with no detectable NR2A or NR2B subunits . ^^^ In contrast , by immunoprecipitation with anti NR 1 17 35 antibodies , which recognise all forms of NR 1 , NR 1 , NR2A , and NR2B immunoreactivities were detected in the immune pellets . ^^^ Similarly , a co association of NR 1 , NR2A , and NR2B subunits was demonstrated following extraction of forebrain membranes with 1 % sodium deoxycholate ( pH 9 ) and purification by anti NR 1 911 920 antibody affinity chromatography . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Concomitant to these events , NMDA receptor subtypes NR 1 and NR2A transiently appeared in hair cells as well as afferent and efferent fibers . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
With the goal to determine quantitatively the subunit composition of cortical NMDA receptors , we used the monoclonal antibody to NR 1 and polyclonal antibodies against the NR2A and NR2B subunits to perform immunoprecipitations of receptor subunits from solubilized adult rat cortical membranes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To determine if these differences might be due to NMDA receptor composition , we compared the expression of NR 1 , NR2A , NR2B , NR2C and NR2D subunit mRNAs in immunocytochemically identified vasopressin and oxytocin neuroendocrine cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To evaluate the involvement of the high affinity binding site in the transient inactivation of the NMDA receptor channels by intracellular calcium , several splice variants of the NR 1 subunit have been individually co transfected with the NR2A subunit in HEK 293 cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Double immunoprecipitation experiments show that all three NMDA receptor subunits that are prevalent in the cortical structures , NR 1 , NR2A , and NR2B , are substrates for PKA as well as PKC . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Memantine blocked and could be trapped by recombinant NMDA receptors composed of NR 1 and either NR2A or NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To evaluate whether changes in the expression of N methyl D aspartate receptor subunits might contribute to these effects , the expression of NR 1 , NR2A , NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analysis of expression of NR 1 , NR2A , and NR2B receptor subunits showed no difference between control and chronic ethanol treated cultures . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Sensitivity to N 1 DnsSpm was reduced by mutation NR 1 ( N616Q ) and was increased by mutations NR 1 ( N616G ) and NR2A ( N615G ) . ^^^ The NR 1 ( N616G ) and NR2A ( N615G ) mutations decreased the Kd ( 0 ) value of N 1 DnsSpm without affecting z delta , whereas the NR 1 ( N616Q ) mutation reduced z delta . ^^^ In contrast , block by N 1 DnsSpm was partially relieved at extreme negative potentials at receptors containing NR 1 ( N616G ) or NR2A ( N615G ) , suggesting that N 1 DnsSpm can permeate these mutant channels but not wild type NR1 / NR2A channels . ^^^ This is hypothesized to be due to an increase in the pore size of channels containing NR 1 ( N616G ) or NR2A ( N615G ) , which allows passage of the bulky head group of N 1 DnsSpm . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Effects of an anabolic androgenic steroid on the regulation of the NMDA receptor NR 1 , NR2A and NR2B subunit mRNAs in brain regions of the male rat . ^^^ The expression of the N methyl D aspartate ( NMDA ) receptor subunits NR 1 , NR2A and NR2B mRNAs was examined in discrete areas of the male rat brain ( including hippocampus , hypothalamus , nucleus accumbens and cortex ) following 14 days daily intramuscular injections of high doses ( 5 and 15 mg / kg ) of an anabolic androgenic steroid ( AAS ) ( nandrolone decanoate ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used in situ hybridization histochemistry to measure the messenger RNA levels for the NR 1 , NR2A and NR2B subunits of the N methyl D aspartate receptor and [ 3H ] dizocilpine maleate ( a non competitive antagonist ) binding to measure N methyl D aspartate receptor density . ^^^ In the intact animal , corticosterone increased messenger RNA levels for NR2A and NR2B but not NR 1 subunits of the N methyl D aspartate receptor in all regions of the hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
No difference was found in the whole tissue expression of messenger RNA for the NR2A , 2B or 2C subunits or for the eight known splice variants of the NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The model is constructed by co expressing glycine / Na / Cl cotransporters ( GLYT1b ) and NMDA receptors ( NMDARs ) ( composed of the subunits NR 1 and NR2A or NR2B ) in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To further investigate the modulatory effects of zinc on NMDA receptors of different subunit compositions , we coexpressed the recombinant subunit NR 1 with NR2A and / or NR2B in HEK 293 cells . ^^^ Cells cotransfected with NR 1 , NR2A , and NR2B showed zinc sensitivity intermediate between that characteristic of NR1 / NR2A and that of NR1 / NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridization using oligonucleotide probes specific for NMDA receptor subunit mRNAs NR 1 and NR2A , B , C , and D revealed no clear differences in brain regional expression between ANT and AT rates . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The essential role of NR 1 and NR2B in development is graphically demonstrated by the neonatal death of transgenic mice lacking either of these two subunits ( Forrest et al . , 1994 ; Kutsuwada et al . , 1996 ) whereas NR2A and NR2C deficient mice are less severely affected ( Sakimura et al . , 1995 ; Ebralidze et al . , 1996 ) . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
High affinity zinc inhibition of NMDA NR 1 NR2A receptors . ^^^ By analyzing the effects of Zn2+ on varied combinations of NR 1 ( NR1a or NR1b ) and NR 2 ( NR2A , NR2B , NR2C ) , we show that both the NR 1 and the NR 2 subunits contribute to the voltage independent Zn2+ inhibition . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
These experiments document an abrupt decrease in the NMDA receptor contribution to synaptic currents that occurs before eye opening and is closely associated with changes in NR 1 protein , rapidly rising levels of the NMDA receptor subunit NR2A , and decreasing levels of NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Supraoptic NMDA receptors thus present characteristics that strikingly resemble those of reconstituted receptors composed of NR 1 and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Molecular dissection of native mammalian forebrain NMDA receptors containing the NR 1 C2 exon : direct demonstration of NMDA receptors comprising NR 1 , NR2A , and NR2B subunits within the same complex . ^^^ The purified receptor subpopulation showed immunoreactivity with anti NR 1 C2 , anti NR 1 N1 , anti NR 1 C2 ' , anti NR2A , and anti NR2B NMDA receptor antibodies . ^^^ The NR 1 C2 receptor subpopulation was subjected to immunoprecipitation using anti NR2B antibodies and the resultant immune pellets analysed by immunoblotting where anti NR 1 C2 , anti NR 1 C2 ' , anti NR2A , and anti NR2B immunoreactivities were all found . ^^^ Of this , 87 % ( i . e . , 40 % of total ) were NR 1 C2 / NR2B receptors and 13 % ( 6 % of total ) were NR 1 C2 / NR2A / NR2B , thus identifying the triple combination as a minor receptor subset . ^^^ They show the coexistence of two splice forms of the NR 1 subunit , i . e . , NR 1 C2 and NR 1 C2 ' , in native receptors and , in addition , they imply an NMDA receptor subpopulation containing four types of NMDA receptor subunit , NR 1 C2 , NR 1 C2 ' , NR2A , and NR2B , which , in accord with molecular size determinations , predicts that the NMDA receptor is at least tetrameric . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The enhanced ifenprodil sensitivity following ethanol exposure was not associated with changes in NR 1 , NR2A , or NR2B immunoreactivity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Single channel recordings were obtained from Chinese hamster ovary cells transfected with the N methyl D aspartate ( NMDA ) receptor subunit NR 1 in combination with NR2A , NR2B , NR2C , or NR2A / NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the current study , structure activity relationships for sulfated steroids were examined in voltage clamped chick spinal cord and rat hippocampal neurons in culture and in Xenopus laevis oocytes expressing NR 1 ( 100 ) and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblotting of native NMDA receptors showed that NR 1 , NR2A , NR2C , and NR2D subunits could all be found in the human spinal cord of which NR 1 was preferentially located to the dorsal half . ^^^ Immunoprecipitation of solubilized receptors revealed that NR 1 , NR2C , and NR2D subunits coprecipitated with the NR2A subunit , indicating that native human spinal cord NMDA receptors are heteroligimeric receptors assembled by at least three different receptor subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The localization of neurons expressing mRNAs for the NR 1 and NR2A D subunits of the glutamatergic NMDA receptor was examined by non radioactive in situ hybridization throughout the guinea pig vestibular nuclei . ^^^ NR 1 and NR2A D mRNAs were colocalized in some but not other neuronal types . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To understand mechanisms of zinc inhibition , cloned heteromeric N methyl D aspartate receptors ( primary subunit NR 1 with secondary subunits NR2A , NR2C or NR2D ) were expressed in Xenopus oocytes and studied under two electrode voltage clamp . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridization histochemistry and immunocytochemistry were used to map distributions of cells expressing mRNAs encoding alpha , beta , gamma , and delta isoforms of type 2 calcium / calmodulin dependent protein kinase ( CaMKII ) , alpha amino 3 hydroxy 5 methyl 4 isoxazoleproprionate ( AMPA ) / kainate receptor subunits , ( GluR 1 7 ) , and N methyl D aspartate ( NMDA ) receptor subunits , NR 1 and NR2A D , or stained by subunit specific immunocytochemistry in the dorsal lateral geniculate nuclei of macaque monkeys . ^^^ NMDA subunits , NR 1 and NR2A , B , and D , are expressed at moderate to low levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 and NR2A were more evenly distributed over neurons of STR and GP Between the different parts of STR and GP , we observed only minor differences in the expression of NRs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We analyzed the cellular expression of the five NR subunit ( NR 1 and NR2A D ) mRNAs in these regions with in situ hybridization and human ribonucleotide probes . ^^^ Film autoradiograms demonstrated a distinct pattern of hybridization signal in the hippocampal complex and the neocortex with probes for NR 1 , NR2A , and NR2B mRNA . ^^^ Pyramidal cells in both hippocampus and neocortex express NR 1 , NR2A , NR2B , and , to a lesser extent , NR2D mRNA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Deactivation time course recorded in response to fast glutamate pulses were studied in isolated and lifted cells , as well as in outside out membrane patches excised from cells expressing recombinant NR 1 subunits in combination with the NR2A , NR2B , NR2C , or NR2D NMDA receptor subunits . ^^^ Channel deactivation was fast and comparable among receptors obtained by cotransfecting five distinct spliced variants of the NR 1 subunit , each with the NR2A subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have investigated the developmental change in NMDA receptor affinity for both glutamate and glycine in acutely dissociated parietal cortex neurons of the rat , together with the expression during ontogeny of NR2A and NR2B mRNA and protein . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have analysed the internal Mg2+ block in recombinant NR 1 NR2A NMDA receptors expressed in Xenopus oocytes . ^^^ We have determined the effects of mutations of two asparagines that line the selectivity filter of the channel , one located within the NR 1 subunit ( N 598 ) and the other within the NR2A subunit ( N 596 ) . 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Although a few studies have examined the composition of NMDA receptors containing NR 1 , NR2A , and NR2B , the composition of native NMDA receptors that incorporate the NR2D subunit is not known . ^^^ The goal of the current study was to examine the subunit composition of native NMDA receptors that contain the NR2D subunit in the rat central nervous system by immunoprecipitation of assembled NMDA receptors from rat brain tissues using specific antibodies against NR 1 , NR2A , NR2B , and NR2D subunits . ^^^ In addition , the NR2D subunit forms a heteromeric assembly with NR 1 , as well as with NR2A and / or NR2B subunits , reflecting ternary complex formation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we describe a novel NR2B selective NMDA antagonist , 4 3 [ 4 ( 4 fluoro phenyl ) 3 , 6 dihydro 2H pyridin 1 yl ] 2 hydroxy propoxy  benzamide ( Ro 8 4304 ) , which exhibits > 100 fold higher affinity for recombinant NR 1 ( 001 ) / NR2B than NR 1 ( 001 ) / NR2A receptors . 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The absence of NMDA and AMPA receptors in rat and human CEC was further confirmed by the lack of immunocytochemical staining of cells by antibodies specific for the AMPA receptor subunits GluR 1 , GluR2 / 3 , and GluR 4 and the NMDA receptor subunits NR 1 , NR2A , and NR2B . ^^^ We failed to detect mRNA expression of the AMPA receptor subunits GluR 1 to GluR 4 or the NMDA receptor subunits NR 1 ( 1XX ) ; NR 1 ( 0XX ) , and NR2A to NR2C in both freshly isolated rat and human microvessels and cultured CEC using reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Primary rat cortical neurons were found to express GluR 1 to GluR 3 and NR 1 , NR2A , and NR2B by both immunocytochemistry and RT PCR and KA 1 , KA 2 , GluR 5 , GluR 6 , and GluR 7 by RT PCR . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The acute effects of subcutaneously injected morphine on transcripts of the NMDA receptor subunits NR 1 , NR2A and NR2B in certain areas of the central nervous system of male rats were examined by Northern blot analysis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The protein levels of the NR 1 and NR2B , but not NR2A , subunits were significantly increased in diazepam withdrawn rats compared to those in control rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Similar results were obtained for two cysteine mutations at asparagine residues of the NR 1 and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
A similar effect was seen with a mutation at the equivalent residue in NR2A but not with mutations at the equivalent residue or adjacent residues in NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The pattern of NR 1 , NR2A , NR2B , and NR2C subunit mRNA in situ hybridization was consistent with previous studies . ^^^ Brain levels of NR 1 and NR2A mRNAs were lowest shortly after birth , increasing to reach peak levels by 14 or 21 days of age and subsequently decreasing at 28 days of age . ^^^ Lead exposure resulted in significant and opposite effects in NR 1 and NR2A subunit mRNA expression with no changes in NR2B or NR2C subunit expression . ^^^ The Pb induced changes in NR 1 and NR2A subunit mRNA were mainly present in the hippocampus . ^^^ NR 1 mRNA levels were also significantly increased ( 14 . 0 % ) in the cerebellum of 28 day old rats with no change in NR2A mRNA at any age . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The function of other ligand gated ion channels , such as the N methyl D aspartate receptor ( NR 1 + NR2A or 2C ) and the 5 HT 3 receptor expressed in Xenopus oocytes , were unaffected by OA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Distribution and expression of the subunits of N methyl D aspartate ( NMDA ) receptors ; NR 1 , NR2A and NR2B in hypoxic newborn piglet brains . ^^^ The purpose of this study was to identify the distribution and the expression of the NR 1 , NR2A and NR2B subunits of the NMDA receptor after cerebral hypoxia . ^^^ Tissue samples from the nine different regions of newborn piglet brain were obtained , and the protein amount of the NR 1 , NR2A , and NR2B subunits measured by immunoblot using the antibody to the NR 1 , NR2A , and NR2B subunits . ^^^ But the expression of the NR 1 , NR2A and NR2B subunits were not significantly different between the control and the hypoxic group , 1 h after hypoxic exposure , indicating no changes in the protein amount of NMDA receptor subunits . ^^^ These results show a significantly higher amount of the NR 1 , NR2A and NR2B subunits in the hippocampus and the cerebral cortex of newborn brains , indicating that these structures could be highly vulnerable to excitotoxicity in the newborn brain . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Human embryonic kidney ( HEK ) 293 cells containing NR 1 1a or NR 1 1b with either the NR2A or NR2B subunits did not differ in their EtOH sensitivity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We investigated the effect of chronic ethanol administration and its withdrawal on the polypeptide levels of NMDA receptor subunits such as NR 1 , NR2A , and NR2B in the rat cerebral cortex and hippocampus using Western blot analysis technique . ^^^ Our results indicate that chronic ethanol treatment upregulates NMDA receptor subunits NR 1 , NR2A , and NR2B ( approximately 35 % ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we show that NR2B , but not NR2A or NR 1 , subunits of NMDA receptors are responsible for autophosphorylation dependent targeting of CaMKII . ^^^ Autophosphorylation induces direct high affinity binding of CaMKII to a 50 amino acid domain in the NR2B cytoplasmic tail ; little or no binding is observed to NR2A and NR 1 cytoplasmic tails . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Our data show that inclusion of exon 5 into the NR 1 subunit increases the IC 50 for voltage independent Zn2+ inhibition from 3 fold to 10 fold when full length exon 22 is also spliced into the mature NR 1 transcript and the NMDA receptor complex contains the NR2A or NR2B subunits ; exon 5 has little effect on Zn2+ inhibition of receptors that contain NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Whereas the modulatory efficacy , but not the potency , of PS is increased two fold by co injection of NR 1 ( 100 ) +NR2A cRNAs as compared with NR 1 ( 100 ) cRNA alone , there is little or no effect of the NR2A subunit on efficacy or potency of pregnanolone ( or epipregnanolone ) sulfate as an inhibitor of the NMDA response . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Quantitative in situ hybridization histochemistry was used to evaluate the expression of NMDA receptor subunits NR 1 and NR2A D in neonatal rats , 2 to 4 h after treatment with MK 801 . ^^^ The percent increase in NR2A mRNA was larger than the percent change in NR 1 , NR2B or NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blot and RNase protection analyses were used to determine the expression of the various NMDA receptor subunits ( NR 1 , NR2A , NR2B , and NR2C ) after BDNF or FGF 2 treatment . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To understand its molecular anatomical basis , we produced affinity purified antibodies against the GluRepsilon 1 ( NR2A ) , GluRepsilon 2 ( NR2B ) , and GluRzeta 1 ( NR 1 ) subunits of the N methyl D aspartate ( NMDA ) receptor channel , and determined their distributions in the mouse hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Clusters of NR 1 and NR2B subunits were observed as early as DIV 3 , while NR2A clusters were rarely observed before DIV 10 . ^^^ At DIV 18 , NR2B clusters partially co localize with those of NR2A subunits , but NR2B clusters always co localize with those of NR 1 subunits . ^^^ Our data indicate that excitatory synapse formation occurs when NMDA receptors comprise NR 1 and NR2B subunits , and that NR2A subunits cluster preferentially at synaptic sites . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR2A or NR2B , in combination with NR 1 ( 011 ) , conveyed enhancement whereas NR2C and NR2D conveyed suppression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Differential effects of electroconvulsive shock on the glutamate receptor mRNAs for NR2A , NR2B and mGluR5b . ^^^ In the dentate gyrus , such treatment elevated the mRNAs for the NMDA subunits NR2A and NR2B , but it reduced the mRNA for the metabotropic glutamate receptor mGlu5b . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we extend those earlier studies by measuring levels of subunit protein for NMDAR 1 , NR2A , and NR2B in the hippocampus of control and kindled animals , 28 days after the last kindled seizure . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast , for NMDAR channels composed of NR 1 and NR2A subunits , the biionic flux ratio exponent was approximately 2 , indicating a deviation from Goldman Hodgkin Katz . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The presence of NMDA receptor protein was determined by Western blot analysis for the NR 1 , NR2A , and NR2B subunits . ^^^ No difference in NR 1 and NR2A subunit protein in the Hipc was detected between the lactating and nonlactating rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This study refines the region of the NR 1 subunit involved in a modulation crucial to the function of NMDA receptors and provides evidence that the NR2A and NR2B subunits realize this modulation differentially . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
By in situ hybridization combined with retrograde Fast Blue labeling , expression of alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid ( AMPA ) receptor ( GluR A to D ) and N methyl D aspartate ( NMDA ) receptor ( NR 1 and NR2A D ) subunit mRNAs were examined in visceromotor and somatomotor neurons of the rat lumbosacral spinal cord . ^^^ In addition to high levels of NR 1 subunit mRNA , dorsolateral nucleus neurons moderately expressed NR2A and NR2B subunit mRNAs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using in situ hybridization techniques , the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NRI , NR2A , and NR2B were investigated . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N Methyl D Aspartate ( NMDA ) receptors containing NR 1 and NR2A subunits have been expressed with high efficiency in Human Embryonic Kidney 293 cells with the aid of a recombinant vaccinia virus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Ischemic preconditioning selectively induces a decrease in the levels of the NR2A and NR2B subunits and a modest decrease in the levels of NR 1 subunit proteins in the synaptosomal fraction of the neocortex but not hippocampus after the second lethal ischemia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA induced changes in free intracellular Ca2+ concentration ( [ Ca2+ ] 1 ) were determined in individual cultured rat mesencephalic neurones by the fura 2 method . mRNA expression encoding NMDA receptor subunits ( NR 1 , NR2A D ) was examined by RT PCR . 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analysis of NMDA ( NR 1 , NR2A , NR2B , and NR2D ) , AMPA ( GluR 1 and GluR2 / 3 ) , and kainate ( GluR6 / 7 ) subunit polypeptides in 3 , 5 , 8 , 10 , and 12 day old cultures showed that NMDA receptor subunits NR 1 , NR2A , and NR2B and AMPA receptor subunits GluR2 / 3 progressively increased as a function of time , whereas levels of NMDA subunit NR2D were high at day 3 and progressively declined to barely detectable levels by day 12 . ^^^ However , exposure of low glutamine cultures to 100 mM ethanol for 4 days ( starting at culture day 9 ) significantly increased the levels of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) and AMPA receptor subunits ( GluR 1 and GluR2 / 3 ) , but had no effect upon kainate receptor subunits ( GluR6 / 7 ) or the synapse associated proteins synapsin 1 and PSD 95 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Changes in NMDA receptor mRNA expression were determined using ribonuclease protection assays targetting the NR2A , 2B , 2C and NR 1 pan subunits as well as the three alternatively spliced NR 1 inserts ( NR 1 pan describes all the known NR 1 splice variants generated from the 5 ' insert and the two 3 ' inserts ) . ^^^ Levels of NR2A , NR2C , NR 1 pan and both 3 ' NR 1 insert mRNAs from the ethanol treated groups did not alter compared with the pair fed control group . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 , NR2A , and NR2B subunit protein levels were measured in rat hippocampus and cortex at post natal days ( PND ) 7 , 14 , 21 , and 28 by Western blot and densitometric analysis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we present evidence from NR 1 mutants and splice variants that Src potentiates NMDA receptor currents by reducing the tonic inhibition of receptors composed of NR 1 and NR2A subunits by extracellular zinc . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We found an increase in expression of the NR2A subunit , and a decrease in expression of the NR2B subunit of the NMDA receptor in primary cultures of cerebellar granule neurons over time in culture , with no significant change in NR 1 expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
However , 1 , 5 ( diethylamino ) piperidine inhibited [ 125I ] MK 801 binding ( IC 50 = 8 . 0 microM ) in NR 1 : NR2A receptors and produced a small increase followed by a modest decrease in binding to NR1a / NR2B receptors . ^^^ Similar effects were demonstrated in both NR 1 : NR2A and NR 1 : NR2B receptors ( IC 50 = 8 . 4 and 14 . 1 microM , respectively ) and agonists decreased the affinity of arcaine in both receptor preparations . ^^^ These results suggest that the stimulatory effects of polyamines on recombinant receptors are influenced by the NR 2 subunit , and that NR 1 : NR2A does not contain a positive modulatory site . ^^^ Furthermore , native NMDA receptors pharmacology can not be modeled by simple NR 1 : NR2A or NR 1 : NR2B combinations . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Radioactive complementary RNA ( cRNA ) probes were prepared from cDNAs specific for alpha amino 3 hydroxy 5 methylisoxozolepropionate ( AMPA ) / kainate ( GluR 1 GluR4 ) , kainate ( GluR 5 GluR7 ) , and N methyl D aspartate ( NMDA ; NR 1 , NR2A NR2D ) receptor subunits . ^^^ AMPA / kainate and NR 1 , NR2A , and NR2B receptor transcripts show higher expression than other transcripts . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of NMDA receptor subunits NR 1 , NR2A , and NR2B was examined in animals ranging in age from postnatal day 16 to adult using Western blotting . ^^^ We observed a remarkable increase in NR 1 and NR2A , but not NR2B , expression after eye opening . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Differential interaction of the tSXV motifs of the NR 1 and NR2A NMDA receptor subunits with PSD 95 and SAP 97 . ^^^ Using the two hybrid genetic system in yeast and site directed mutagenesis , we compared the binding of the NR2A , NR 1 3 and NR 1 4 tSXV motifs with the PDZ domains of PSD 95 and SAP 97 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we investigated the co expression of the neurotrophin 4 / brain derived neurotrophic factor receptor TrkB with the N methyl D aspartate glutamate receptor subunits NR 1 C1 , NR2A and NR2B , on postnatal days 10 and 22 and in the adult rat primary visual cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study was undertaken to examine the level of gene expression for the NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A receptor subunits in isolated regions of rat brain using the nuclease protection assay . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptor NR 1 and NR2A / B subunit expression in trigeminal neurons during early postnatal development . ^^^ Receptor immunohistochemistry was used to describe changes in the expression of NMDA NR 1 and NR2A / B receptor subunits in Mo 5 , Me 5 , Su 5 , and I 5 neurons during postnatal development . ^^^ An affinity purified polyclonal antibody against the NR 1 subunit and an affinity purified polyclonal antibody that recognizes both NR2A and 2B subunits were used to depict the expression of these subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RESULTS : The transcript levels of NR 1 and NR2A subunits of N Methyl D Aspartate were unchanged in rats with a diabetic duration of 3 months when compared with age matched control rats . ^^^ Accordingly , NR 1 and NR2A as well as GluR 1 , GluR2 / 3 , PSD 95 and alphaCaMKII protein concentrations in post synaptic densities were the same in both control and diabetic rats , whereas the immunoreactivity for NR2B was reduced by about 40 % . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors consist of two distinct groups of subunits : NR 1 , that is ubiquitously in the receptor complexes ; and NR2A D , that differentiate and potentiate NMDA receptor responses by assembling with NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To relate this distribution to that of NR 1 and to the NMDA receptor anchoring protein PSD 95 , we documented extensive cellular colocalization of NR2A / B with NR 1 at the light microscopic level . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N to Q and N to R mutations in the N site of the NR 1 subunit ( N 598 in NR 1 ( 011 ) , N 619 in NR 1 ( 100 ) ) and N to Q mutations in the N and N + 1 sites ( N 595 and N 596 in NR2A , respectively ) of the NR 2 subunit ( Q / NN , R / NN , N / QN , N / NQ , Q / QN and Q / NQ receptors ) reduced affinity for phencyclidine . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus , human embryonic kidney 293 cells were transfected with the NR 1 1a and NR2A NMDA receptor subunits in combination with both FLAG and c Myc epitope tagged NR2B subunits . ^^^ Immunoblotting of the double immunopurified NR2A / NR2B ( FLAG ) containing material demonstrated the presence of anti NR 1 , anti NR2A , anti FLAG , and , more important , anti c Myc antibody immunoreactivities . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Hippocampal sclerosis ( HS ; n = 16 ) , non HS ( n = 10 ) , and autopsy hippocampi ( n = 9 ) were studied for NMDAR 1 ( NR 1 ) and NR2A D mRNA levels by using semiquantitative in situ hybridization techniques , along with neuron densities . ^^^ These findings indicate that chronic temporal lobe seizures are associated with differential changes in hippocampal NR 1 and NR2A D hybridization densities that vary by subfield and clinical pathological category . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we evaluated whether the two redox sites act independently of each other , with the NR 1 site affecting the opening frequency and the NR2A site altering open dwell time . ^^^ Dithiothreitol increased the open dwell time and opening frequency of NR 1 ( C744A , C798A ) / NR2A receptors in a manner indistinguishable from that previously seen in wild type channels . ^^^ Sulfhydryl reagents modulated NR 1 ( C744A , C798A ) / NR2A receptors identically to wild type NR1 / NR2A channels , whereas NR 1 ( C744A , C798A ) / NR2C receptors were insensitive to redox modulation . ^^^ We conclude that cysteines 744 and 798 on the NR 1 subunit are not involved in the redox modulation of NR1 / NR2A receptors , but are crucial for the modulation of NR1 / NR2C containing receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDARs at birth are comprised of NR2B and NR 1 subunits , and , over the first 5 postnatal weeks , there is a progressive inclusion of the NR2A subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 and NR2A mRNA increased continuously over time in culture , whereas NR2B mRNA increased dramatically during the first 10 days and subsequently remained stable . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Analysis of NMDA receptor subunits shows a persistent , approximately 30 50 % reduction of NR 1 , NR2A , and NR2B subunits at postnatal day 90 in the barrel field cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
With voltage clamp recording of Xenopus oocytes expressing heteromeric NMDA receptors from cloned NR 1 and NR 2 subunit RNAs , Con R exhibited the following order of preference for NR 2 subunits : NR2B approximately NR2A > NR2C > > NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study was undertaken to determine the role in PKC induced potentiation of the NR 1 and NR2A C terminal tails , which serve as targets of PKC phosphorylation [ Tingley , W . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In lesioned striatum , the abundance of NR 1 and NR2B in striatal membranes was decreased to 68 + / 3 . 2 and 62 + / 4 . 4 % , respectively , relative to the unlesioned striata , whereas the abundance of NR2A was unchanged . ^^^ The phosphorylation of NR 1 protein at serine 890 and serine 896 , but not at serine 897 , and the tyrosine phosphorylation of NR2B but not NR2A were decreased in the membrane fraction of the lesioned striatum . ^^^ Chronic treatment of lesioned rats with L dopa normalized the alterations in the abundance and subunit composition of the NMDA receptors in striatal membranes , and produced striking hyperphosphorylation , both of NR 1 at serine residues , and NR2A and NR2B at tyrosine residues . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoreactive bands corresponding to the NMDA receptor subunits NR 1 , NR2A , NR2B , NR2C and NR2D were demonstrated by immunoblotting at the earliest between 4 . 5 and 7 weeks and increasing concentrations were seen up to 11 weeks of gestation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of NR 1 , NR2A D , and NR 3 subunits of the NMDA receptor in the cerebral cortex and olfactory bulb of adult rat . ^^^ Quantitative reverse transcriptase polymerase chain reaction was used to analyze the relative expressions of NR 1 , NR2A , NR2B , NR2C , NR2D , and NR 3 subunits of the NMDA receptor in the piriform , entorhinal , visual , and motor cortices as well as in the olfactory bulb of adult rat . ^^^ The expression of NR2A was only slightly higher than half of that of NR 1 . ^^^ NR2A had the levels of expression of approximately 50 % of those of NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Neurons pretreated with BDNF exhibited increased levels of the NMDA receptor subunits NR 1 and NR2A , which was associated with increased calcium responses to NMDA and vulnerability to excitotoxic necrosis and reduced vulnerability to apoptosis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Because native NR2A and NR2B containing receptors are pharmacologically distinct , it should be possible to develop NR2A and NR2B selective glutamate site antagonists . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Consistently , exposure to insulin increased the level of the NR2A subunit of the NMDA receptor without much altering NR 1 or NR2B levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Postnatal development of NR 1 , NR2A and NR2B immunoreactivity in the visual cortex of the rat . ^^^ The NMDAR subunits NR 1 , NR2A and NR2B are developmentally regulated , and it has been proposed that developmental changes in their expression may underlie developmental changes in cortical plasticity . ^^^ We hypothesized that there will be significant age dependent change in expression of NR 1 , NR2A or NR2B between P 22 , when the cortex is plastic , and P 90 , when it is not . ^^^ We applied specific antibodies recognizing NR 1 , NR2A and NR2B to the primary visual cortex at P 14 , P 22 , P 30 , P 45 and P 90 . ^^^ We found age dependent changes in NR 1 IR that were negatively correlated with changes in NR2A IR ; these subunits are not regulated in unison . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Finally , chronic MK 801 administration did not alter NMDA receptor NR 1 , NR2A , or NR2B subunit peptide expression in either the cerebral cortex or the hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunohistochemical localization of N methyl D aspartate receptor NR 1 , NR2A , NR2B and NR2C / D subunits in the adult mammalian cerebellum . ^^^ The distributions of the N methyl D aspartate ( NMDA ) receptor NR 1 , NR2A , NR2B and NR2C / D subunits were mapped in adult mouse cerebellum using subunit specific antibodies . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We used a dual label in situ hybridization technique to assess the levels of NR 1 and NR2A D messenger ribonucleic acid ( mRNA ) expressed in projection neurons and interneurons of the human striatum . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This neurotoxic effect could be totally prevented by preincubation with an N methyl D aspartate receptor subunit 2B ( NR2B ) specific antagonist , NR2B antibodies , and an unspecific N methyl D aspartate receptor blocker and was partially blocked with an NR2A specific antagonist but not with NR2A antibodies or alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptor and metabotropic glutamate receptor antagonists . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We now report that levels of AMPA receptor subunits GluR 1 and GluR 4 are rapidly decreased in cultured rat hippocampal neurons undergoing apoptosis in response to withdrawal of trophic support ( WTS ) , whereas levels of NMDA receptor subunits NR 1 , NR2A , and NR2B are unchanged . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , co expression of the NR2A subunit and an NR 1 subunit that lacks the alternatively spliced intracellular C 1 cassette did not reduce the effects of ethanol on channel function as measured by patch clamp electrophysiology . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using in situ hybridization , we found a selective increase of the subunits NR 1 and NR2A mRNA at 24 h in ventral motor neurons ( VMN ) caudal to a standardized spinal cord contusion injury ( SCI ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Development of NR 1 , NR2A and NR2B mRNA in NR 1 immunoreactive cells of rat visual cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridization autoradiography was used to quantify mRNA for the NMDA receptor subunits NR 1 , NR2A , and NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Developmental changes in the expression of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) in the cat visual cortex and the effects of dark rearing . ^^^ The present study used Western blots to determine changes in the level of expression of the three major NMDA receptor subunits , NR 1 , NR2A , and NR2B , in relation to the ' critical period ' in cat visual cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA induced currents recorded from XENOPUS : oocytes injected with cRNA encoding the NMDA receptor subunits NR 1 , NR2A , and / or NR2B , mainly found in rat hypothalamus , were not affected by PKA activation but were increased by protein kinase C ( PKC ) stimulation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In order to investigate whether functional receptors may contain more than one sort of NR 2 subunit , NR 1 , NR2A and NR2D subunits were co expressed in Xenopus oocytes . ^^^ It is postulated that the novel channel contains NR 1 , NR2A and NR2D subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors are multimeric associations of NR 1 and NR2A NR2D subunits that form ligand gated ion channels . ^^^ NR 1 was the most abundant subunit mRNA ; NR2A , NR2B , and NR2D subunit mRNAs were also present , but were expressed in nucleus specific patterns . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunohistochemistry was performed on retinal sections at different developmental ages between embryonic ( E ) days 20 / 21 and the adult stage using specific antibodies against NMDA subunits ( NR 1 , NR2A D ) . ^^^ NR 1 was found in two bands which can be related to the off and on signal pathways , whereas NR2A and NR2B were located in two bands within the off sublaminae of the IPL . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RESULTS : Ninety three percent of retinal ganglion cells expressed mRNA coding for NR 1 , 9 % for NR2A , 28 % for NR2B , 86 % for NR2C , and 84 % for NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Recombinant NR1a / NR2A ( T671A ) N methyl D aspartate ( NMDA ) receptor channels , which carry a point mutation in the putative glutamate binding site that reduces glutamate potency by around 1000 fold , have been expressed in Xenopus laevis oocytes and their single channel properties examined using patch clamp recording techniques . ^^^ The single channel data that we present in this paper support further the idea that the point mutation T671A in the NR2A NMDA receptor subunit affects mainly the ability of glutamate to remain bound to these channels . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Intracerebroventricular injection of the N terminal substance P fragment SP ( 1 7 ) regulates the expression of the N methyl D aspartate receptor NR 1 , NR2A and NR2B subunit mRNAs in the rat brain . ^^^ We have examined the effect of intracerebroventricular injections of SP ( 1 7 ) on the expression of the NMDA receptor subunits NR 1 , NR2A and NR2B mRNAs in the spinal cord and in discrete areas of the male rat brain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
As demonstrated by chronic blockade of postsynaptic glutamate receptors in vitro , the regulation of NR2A mRNA was strongly dependent on synaptic activity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Changes in the expression of the AMPA receptor subunits GluR 1 4 and of the NMDA receptor subunits NR 1 , NR2A D were investigated in the developing rat medial and lateral vestibular nuclei . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Previously we reported up regulation of NMDA receptor subunits NR 1 and NR2A mRNAs in VMN caudal to the lesion site 24 h after SCI . ^^^ We found increased NR 1 and NR2A mRNAs in VMN similar in extent to that seen after SCI . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
GluR 1 , GluR2 / 3 , GluR 4 , NR 1 ) appear to be stable for up to 18 h post mortem , the NR2A and NR2B subunits appear to be proteolyzed rapidly following death . ^^^ Modest decreases in NMDA receptor subunits NR 1 , NR2A , and NR2B were found in the hippocampus and in frontal cortex while little or no change in any of these subunits were documented in entorhinal cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Message levels for the NMDA receptor subunits NR 1 , NR2A , and NR2B were not different between the chemically challenged neurons and control cells , whereas NR2C message was barely detectable in either group . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We investigated the influence of centrally administered ginsenoside on the regulation of mRNA levels of the family of NMDA receptor subtypes ( NR 1 , NR2A , NR2B , NR2C ) by in situ hybridization histochemistry in the rat brain . ^^^ However , there was no significant change of NR 1 and NR2A mRNA levels in Rc infused rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) analysis indicated that GluR 1 , GluR 5 , GluR 7 , EAA 2 , NR 1 , NR2A and NR2D mRNAs were present in the cultured cells . ^^^ Northern analysis confirmed the presence of GluR 7 , EAA 2 , NR 1 , NR2A and NR2D mRNAs , while other mRNAs were under the detection limit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Early post natal deafening decreased bilaterally the expression of mRNAs encoding NR 1 , NR2a , NR2b and flop isoforms of AMPA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study was designed to clarify the role of the NR 1 , NR2A and NR2B subunits of N methyl D aspartate receptors in the development of morphine induced place preference using specific antibodies to N methyl D aspartate receptor subunits in the mouse . ^^^ An i . c . v . injection with specific antibodies against the carboxyl terminal region of either NR 1 , NR2A or NR2B subunits caused the attenuation of seizures induced by i . v . infusion of N methyl D aspartate in the mouse . ^^^ Using western blotting , NR 1 , NR2A and NR2B subunits were found to be highly expressed in the cerebral cortex and hippocampus of the mouse brain , which are key areas in producing seizures regulated by N methyl D aspartate receptors . ^^^ Under these conditions , i . c . v . treatment with the antibody against NR2B subunits abolished the morphine induced place preference , whereas antibodies against NR 1 and NR2A subunits did not affect the rewarding effect of morphine . ^^^ Furthermore , the NR2B subunit protein was specifically up regulated in the limbic forebrain of morphine conditioned mice , whereas the protein levels of either NR 1 or NR2A subunits were not altered . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Protein levels and phosphorylation status of NMDA receptor subunits NR 1 , NR2A and NR2B were correlated with measurements of cognitive performance . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The effects of continuous infusion of butorphanol on the modulation of NMDA receptor subunit NR 1 , NR2A , NR2B , and NR2C gene expression were investigated by using in situ hybridization technique . ^^^ Continuous intracerebroventricular ( i . c . v . ) infusion with butorphanol ( 26 nmol / microl / h ) resulted in significant modulations in the NRI , NR2A , and NR2B mRNA levels . ^^^ No changes of NR 1 , NR2A , NR2C subunit mRNA in the cerebellar granule cell layer were observed in either butorphanol tolerant or withdrawal rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
PMA treatment also produced an increase in the phosphorylation of serine 890 on the NR 1 subunit , a known PKC site , at 5 min with phosphorylation returning to near basal levels by 10 min while tyrosine phosphorylation of NR2A and NR2B was sustained for up to 15 min . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we show that NR3A coassembles with NR 1 1a and NR2A to form a receptor complex with distinct single channel properties and a reduced relative calcium permeability . ^^^ NR3A associates independently with both NR 1 1a and NR2A in the endoplasmic reticulum , but only heteromeric complexes containing the NR 1 1a NMDA receptor subunit are targeted to the plasma membrane . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using site directed mutagenesis , we identified three pairs of cysteine residues that underlie the various kinetic components of redox modulation of NMDA evoked currents in Xenopus oocytes expressing NR1 / NR2A receptors : ( 1 ) Cys 87 and Cys 320 in NR2A underlie the fast component , ( 2 ) Cys 79 and Cys 308 in NR 1 underlie the intermediate component , and ( 3 ) Cys 744 and Cys 798 in NR 1 underlie the persistent component . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In more severely injured rats , the chronic recovery of detrusor EUS coordination was very incomplete and correlated to decreased innervation of lower motoneurons by descending control pathways and their increased levels of mRNA for glutamate receptor subunits NR2A and GluR 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Molecular cloning studies have revealed that NMDA receptors consist of two families , the NR 1 and NR2A NR2D subunits , and NMDA receptors are thought to be pentameric or tetrameric complexes of the NR 1 subunit with one or more of the NR 2 subunits . ^^^ An intracerebroventricular ( i . c . v . ) treatment with a specific antibody against the carboxyl terminal region of the NR2B subunit abolishes the morphine induced place preference , whereas antibodies against the NR 1 and NR2A subunits do not affect the rewarding effect of morphine , indicating that the blockade of the NR2B subunit suppresses the development of the morphine induced rewarding effect . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA subunits NR 1 and NR2A were significantly and rapidly up regulated in VMN after SCI , but only caudal to the lesion site , while VMN loss is similar rostral and caudal to the epicenter . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Since NR2A is not transported to the cell surface unless it is associated with NR 1 ( McIlhinney , R . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The results indicate that : ( 1 ) no single cassette of NR 1 is differentially enriched in the postsynaptic densities and ( 2 ) the NR2A and NR2B subunits are similarly enriched at the synapse . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Following NMDA insult , the mRNA levels for NR 1 ( com ) , NR2A , NR2B and , to a lesser extent , the NR2C subunit were substantially reduced within 24 hr post treatment ( PT ) , and remained depressed for up to 48 hr . ^^^ Protein levels for NR 1 ( com ) , NR2A and NR2B were also monitored at select time points following NMDA insult . ^^^ By day 10 , the levels of protein expression for NR 1 ( com ) and NR2B remained suppressed despite the rise in gene expression for these two subunits , whereas protein for NR2A showed a substantial rise in expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NR 1 subunit of the N methyl D aspartate receptor can be efficiently expressed alone in the cell surface of mammalian cells and is required for the transport of the NR2A subunit . ^^^ We have used a heterologous system of expression of N methyl D aspartate ( NMDA ) receptors based on the use of vaccinia virus to analyse the maturation , transport , assembly and differential expression of the NR 1 and NR2A subunits of the receptors . ^^^ We have demonstrated that the NR 1 subunit is efficiently transported to the plasma membrane in cells expressing NR 1 alone , similarly to cells producing NR 1 and NR2A together . ^^^ In contrast , NR2A requires NR 1 expression to be located at the cell surface . ^^^ The stability of both receptor subunits expressed alone is similar to that obtained in cells producing NR 1 and NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RESULTS : In situ hybridization of riboprobes indicated that , in brain sections from both WSP and WSR mice , there was a differential regional distribution of mRNA for the mouse NR 1 , NR2A , NR2B , and NR2C NMDA receptor subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptor ( NMDAR ) is a hetero oligomeric protein composed of two classes of subunits , the essential subunit NR 1 and NR2A to D subunits that do not by themselves produce functional channels but potentiate NR 1 activity and confer functional variability to the receptor . ^^^ In situ hybridization on isolated rabbit osteoclasts and MG 63 cells has confirmed the localization of NR 1 , NR2B , and NR2D transcripts in osteoclasts and NR 1 , NR2A , NR2B , and NR2D transcripts in MG 63 cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We found that the NMDA receptor ( NR ) subunits NR 1 , NR2A and NR2B were expressed in all three cultures . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 , NR2A , and NR2B mRNA levels were measured by RNase protection assay in young ( 3 4 month ) , middle aged ( 12 13 month ) , and aged ( 24 25 month ) Sprague Dawley rats in different phases of the estrous cycle in cycling animals and in acyclic subjects . ^^^ Age related changes were observed in NR 1 , NR2A , and NR2B in the ventral hippocampus and in NR 1 and NR2B in the dorsal hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
As a result , intense immunolabelling for GluRepsilon 1 ( NR2A ) , GluRepsilon 3 ( NR2C ) , and GluRzeta 1 ( NR 1 ) all appeared in synaptic glomeruli of the granular layer . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
A crude synaptic membrane fraction ( SPM ) was prepared and assayed with antibodies specific for either the NR 1 , NR2A or NR2B subunits . ^^^ Relative to control animals raised in a diurnal light dark cycle , a period of 1 week of dark rearing caused an increase in the relative amount of NR 1 , a decrease in the level of NR2A , and no change in the level of NR2B subunit expression in postnatal day 12 rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The dopamine D 1 receptor agonist SKF 82958 produces ( 1 ) an increase in NR 1 , NR2A , and NR2B proteins in the synaptosomal membrane fraction ; ( 2 ) a decrease in NR 1 , NR2A , and NR2B proteins in the light membrane and synaptic vesicle enriched fractions ; and ( 3 ) an increase in the tyrosine phosphorylation of NR2A and NR2B in the synaptosomal membrane compartment . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Therefore , the present study was designed to investigate the effects of short and long term estrogen replacement or deprivation on mRNA levels of three NMDA receptor subunits , NR 1 , NR2A , and NR2B , in the hippocampus of aging female Sprague Dawley rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Within the anti calnexin immunoprecipitates , no NR2A , NR2B or NR2C subunits were detectable , but a significant pool of NR 1 subunits was identified . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The effects of continuous infusion of NMDA receptor antagonist MK 801 on the modulation of NMDA receptor subunits NR 1 , NR2A , NR2B , and NR2C were investigated by using in situ hybridization study . ^^^ Continuous intracerebroventricular ( i . c . v . ) infusion with MK 801 ( 1 pmol / 10 microl / h ) for 7 days resulted in significant modulations in the NR 1 , NR2A , and NR2B mRNA levels without producing stereotypic motor syndromes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we report that the fast desensitization of NR1 / NR2A receptors is caused by ambient zinc , and that a positive allosteric interaction occurs between the extracellular zinc binding site located in the amino terminal domain and the glutamate binding domain of NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This region in NR 1 and NR2B controls the action of NR2B selective drugs like ifenprodil , while this domain in receptors containing the NR2A subunit controls the action of NR2A selective drugs such as zinc . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Levels of NR 1 and NR2A subunit mRNA were significantly increased in the parietal cortex ( 8 . 4 and 10 . 6 % , respectively ) and hypothalamus ( 8 . 7 and 15 . 2 % , respectively ) in mu opioid receptor knockout mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Furthermore , our biochemical experiments showed that enrichment significantly increased protein levels of GluR 1 , NR2B , and NR2A subunits of glutamate receptors in both wild type and NR2B Tg mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Unexpectedly , we found that expression of NR 1 ( including the expression of NR 1 subunits containing the N 1 , C 1 , and C 2 inserts ) , NR2A , NR2B , NR2C , GluR 1 , GluR2 / 3 , GluR 5 , GluR6 / 7 , and KA 2 subunits was not altered in hippocampal homogenates from ethanol exposed rats . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Finally , although an intact C terminal domain is neither necessary for interaction with the NR2A subunit nor surface expression of the NR 1 4 subunit , the C terminal domain fragment alone blocks surface expression of native NR 1 4 , in a dominant negative fashion , when the two are coexpressed . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Since the sites for tyrosine phosphorylation and nitration are on adjacent carbon atoms , the present study tested the hypothesis that in vitro dephosphorylation of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increases receptor nitration by peroxynitrite in cortical membranes of newborn piglets . ^^^ Nitration was measured by immunoprecipitating with agarose conjugated anti nitrotyrosine antibody followed by Western blot analysis using specific anti NR 1 , NR2A and NR2B primary antibodies . ^^^ The data demonstrate that nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increases following dephosphorylation in both normoxic and hypoxic animals , however increase is much higher in hypoxic animals . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Parallel measurements of free glutamate and homocysteine levels in the patients blood plasma did not reveal any substantial changes of the given parameters , comparing to indices of autoantibodies to NR2a glutamate receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
They were designated as the NR 1 ( sigma 1 ) , NR2A ( epsilon 1 ) , NR2B ( epsilon 2 ) , NR2C ( epsilon 3 ) , and NR2D ( epsilon 4 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
This study was aimed to investigate gene expression of N methyl D aspartate ( NMDA ) receptor subunits ( NR 1 , NR2A , NR2B ) in rat adrenal gland under basal and stress conditions , using RT PCR . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The increase in the NR 1 , NR2A , and NR2B receptor mRNAs started at 5 h , maintained for 1 7 days ( P < 0 . 05 0 . 001 ) and returned to the control level at 14 days after inflammation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Lithium pre treatment did not alter total protein levels of NR 1 , NR2A and NR2B subunits of NMDA receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Whereas PS potentiated NMDA , glutamate , and glycine induced currents of NR1 / NR2A and NR1 / NR2B receptors , it was inhibitory at NR1 / NR2C and NR1 / NR2D receptors . 3 . ^^^ The NR 1 subunit can combine with NR2A , NR2B , NR2C , or NR2D subunits to form functional receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Semi quantitative Western blot techniques were applied with the use of antibodies that recognize either the epsilon 1 ( NR2A ) , epsilon 2 ( NR2B ) or zeta 1 ( NR 1 ) subunits of the NMDA receptor or a synaptic terminal protein , synaptophysin . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NMDA receptor subunits , NR 1 , NR2A , NR2B , NR2C , and NR2D have been cloned in several species , including man . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , temporal changes in NMDA receptor subunit ( NR 1 , NR2A , and NR2B ) levels in rat hippocampus after TBI were investigated by Western blot and mRNA expression levels by RT PCR methods . ^^^ Western blot analysis revealed significant decreases of NR 1 , NR2A , and NR2B subunit proteins at 6 and 12 hr postinjury in rat hippocampus . ^^^ Complete recovery of NR 1 , NR2A , and NR2B subunit protein to the levels of sham controls was observed at 24 hr postinjury . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Fifteen days after transfection , well defined GFP clusters were observed for both GFP NR2A and GFP NR2B subunits being co localized with endogenous NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast , no high affinity specific binding was detected to NR 1 , NR2A , NR2B subunits expressed alone or NR1 / NR2A receptors . ^^^ HEK 293 cells were transfected with NR 1 , NR2A and NR2B receptor subunits and complexes comprising all three subunits were isolated by anti NR2A immunoaffinity chromatography . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blotting analysis demonstrated that the expression of NR2A decreased 2 3 days after in vivo axotomy , while expression of NR 1 and NR2B , NR2C and NR2D subunits did not change significantly . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 and NR2A subunit proteins were detected both in nondifferentiated progenitor cells and in neurons , while the mature form of NR2B subunit protein appeared only at the time of neuronal process elongation . ^^^ Despite the early presence of NR 1 and NR2A subunits , NMDA evoked responses could be detected in NE 4C neurons only after the sixth day of induction , coinciding in time with the expression of the mature NR2B subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blots indicated that rat dorsal root ganglia ( DRG ) contain NR 1 , NR2B , NR2C , and NR2D but not NR2A . ^^^ Immunofluorescence with an antibody that recognized NR 1 and another that recognized NR2A and NR2B showed that NR 1 and NR2B colocalized in 90 % of DRG neurons , including most A fibers ( identified by the presence of neurofilament 200 kDa ) . ^^^ The percentage of cells immunoreactive for NR 1 , NR2A / NR2B , and NR2C / NR2D were the same in the T 9 , T 12 , L 4 , and L 6 DRG . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Animals subjected to brief hyperthermic heat shock ( HS ; 42 degrees C for 15 min ) exhibited augmented expression of NR 1 or NR2A subunit of N methyl D aspartate ( NMDA ) receptors , GluR 1 or GluR 4 subunits of alpha amino 3 hydroxy 5 methylisoxazole 4 propionate receptors and KA 1 subunit of kainate receptors in the NTS . ^^^ These findings suggest that HSP 70 induced by HS may enhance BRR response by up regulating the molecular synthesis and functional expression of NR 1 or NR2A subunit of NMDA receptors and GluR 1 , GluR 4 , or KA 1 subunit of non NMDA receptors in the NTS . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In general , oestrogen had inhibitory effects on NR 1 and NR2a , and differential effects on NR2b subunit mRNA levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analyses showed that levels of the N methyl d aspartate ( NMDA ) subunits NR 1 and NR2A were significantly decreased in neurons exposed to phenformin , whereas levels of the AMPA receptor subunit GluR 1 were unchanged . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Studies were conducted to evaluate the effects of s . c . injected recombinant human growth hormone ( GH ) on the expression of the gene transcript of N methyl D aspartate receptor subunits type 1 ( NR 1 ) , type 2A ( NR2A ) , and type 2B ( NR2B ) in the male rat hippocampus . ^^^ In elderly adult rats ( 57 67 weeks of age ) GH induced an increase in the expression of the hippocampal message for NR 1 and NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To test this hypothesis the effect of hypoxia on the nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor was determined . ^^^ There was a significant increase in the nitrated NR 1 , NR2A and NR2B subunits following hypoxia : 104+ / 11 vs . 275+ / 18 optical density ( OD ) xmm ( 2 ) for NR 1 ( P < 0 . 05 ) , 212+ / 36 vs . 421+ / 16 ODxmm ( 2 ) for NR2A ( P < 0 . 05 ) and 246+ / 44 vs . 360+ / 26 ODxmm ( 2 ) for NR2B ( P < 0 . 05 ) . ^^^ This increase in nitrated NR 1 , NR2A and NR2B subunits of the NMDA receptor was prevented by the administration of NNLA prior to hypoxia ( NR 1 160+ / 19 , P=NS , NNLA vs . normoxic ; NR2A 304+ / 49 , P=NS , NNLA vs . normoxic , and NR2B 274+ / 19 , P=NS , NNLA vs . normoxic ) . ^^^ The increase in nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increased as a function of decreased cerebral high energy phosphates , ATP and phosphocreatine , during hypoxia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Protein levels of NR 1 , phosphorylated NR 1 897serine , NR2A and NR2B in striatum were measured by immunoblotting after piglets underwent hypoxic asphyxic cardiac arrest , cardiopulmonary resuscitation , and recovery for 3 , 6 , 12 or 24 h . ^^^ In membrane fractions isolated from total striatum , mean NR 1 and NR2A levels did not change significantly with time after hypoxia ischemia compared to control ; however , the levels of both NR 1 and phosphorylated NR 1 897serine correlated with neuronal injury in putamen , with higher levels associated with greater neuronal injury in individual animals . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analyses suggest that the hyperfunctional NMDA response is at least partially mediated by an increased synthesis of NR 1 and NR2A subunits as well as a change in the subunit stoichiometry of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) was used to determine the messenger RNA ( mRNA ) levels of the NMDA receptor subunits NR 1 , NR2A , and NR2B in the hippocampus and entorhinal cortex of postmortem brain samples from nine clinically well characterized AD patients and nine aged controls . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To elucidate mechanisms controlling the number and subunit composition of synaptic NMDA Rs in hippocampal slice neurons , the NR 1 , NR2A , and NR2B subunits were optically and electrophysiologically tagged . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Colocalization of the three NMDAR subunits that are most abundant in the hypothalamus ( NR 1 , NR2A , or NR2B ) with GnRH perikarya was determined in female rats at different stages of the reproductive life cycle : young ( 3 4 months ) rats with regular estrous cycles , middle aged ( 8 10 months ) rats with regular estrous cycles , middle aged rats with irregular estrous cycles , and middle aged acyclic rats in persistent estrus . ^^^ The number , percent , and localization of GnRH perikarya expressing NR 1 , NR2A , or NR2B were mapped and quantified by double label immunofluorescence microscopy . ^^^ There were no age or reproductive status related changes in coexpression of NR 1 or NR2A subunits in GnRH neurons . ^^^ This may result in an age related increase in the ratio of the NR2B to the NR 1 and NR2A subunits on GnRH neurons . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We examined the expression of the NR 1 and NR2A subunits of the N methyl D aspartate ( NMDA ) receptor , and the GluR 2 subunit of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate ( AMPA ) receptor , in the ipsilateral and contralateral vestibular nucleus complexes ( VNCs ) at 10 h and 2 weeks following unilateral vestibular deafferentation ( UVD ) in rats , in order to directly test the hypothesis that the behavioural recovery following UVD ( ' vestibular compensation ' ) is associated with an up regulation of NMDA receptors . ^^^ We found no significant changes in NR 1 or NR2A expression at 10 hs or 2 weeks post op . compared to sham and anesthetic controls . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
METHODS : N methyl D aspartate gated currents were measured using whole cell voltage clamp recording in cortical neurons cultured for 1 4 weeks and HEK 293 cells transiently expressing NR 1 1a + NR2A or NR 1 1a + NR2B subunit containing receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Electrophysiological experiments revealed that homoquinolinate activated human recombinant NR1a / NR2A , NR1a / NR2B and NR1a / NR2A / NR2B receptors with EC 50 values of 25 . 2 , 13 . 8 and 9 . 04 micro m , respectively , with intrinsic activities of 148 , 93 . 3 and 125 % , respectively , compared to glutamate ( =100 % ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR2A levels in the right hemisphere of P 7 pups were markedly reduced after 24 h of recovery , while NR 1 and NR2B remained unchanged . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Formalin treatment did not affect NR 1 splice variant expression but significantly increased and decreased the proportion of NR2A and NR2C , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present paper , we investigated whether the expression of NMDAR subunits NR 1 , NR2A , NR2B is influenced by neurotrophins in rat visual cortex . ^^^ Different antibodies were used to stain neurons expressing NR 1 , NR2A and NR2B . ^^^ The staining level for both NR2A and NR2B , but not NR 1 , was reduced in all cortical layers in TrkB IgG treated animals . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Overexpression of either NR2A or NR2B , but not splice variants of NR 1 , by transfection caused a significant increase in the total number of functional NMDA receptors and in surface NR 1 subunit cluster density in CGCs in primary culture . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using the anti NR3A antibody in immunoprecipitation studies , we find that 9 . 7 + / 0 . 8 % of NR 1 , 8 . 7 + / 1 . 8 % of NR2A , and 5 . 0 + / 0 . 6 % of NR2B are associated with NR3A at postnatal day 10 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Two regions in the D 1 receptor carboxyl tail can directly and selectively couple to NMDA glutamate receptor subunits NR 1 1a and NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
A 30 % reduction in alpha amino 3 hydroxy 5 methyl 4 iso xazolepropionate ( AMPA ) GluR 1 and 2 subunit immunoreactivities was observed in the preconditioned cortex ( p < 0 . 03 ) , but there was no significant change in the NMDA receptor subunits , NR 1 , NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analyses of cell homogenates indicated upregulation of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) , but downregulation of synaptic organizing proteins ( PSD 95 , densin 180 , and septin 6 homologue ) and a synapse enriched enzyme ( alphaCaMKII ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Delta catenin forms stable complexes with excitatory neurotransmitter receptors including ionotropic N methyl D aspartic acid receptor 2A ( NR2A ) , metabotropic glutamate receptor 1alpha ( mGluR1alpha ) , as well as PSD 95 in vivo . ^^^ Upon glutamate receptor activation , delta catenin becomes down regulated and its association with NR2A and mGluR1alpha in cultured neurons is diminished . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Real time PCR data revealed that striatal NOS interneurons express the mRNAs encoding NR 1 , NR2A , NR2B , and NR2D but not NR2C . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridization histochemistry was used to determine the mRNA levels of the NMDA receptor subunits NR 1 , NR2A , NR2B , the AMPA receptor subunit GluR 1 , and the kainate receptor subunit KA 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The N methyl d aspartate ( NMDA ) glutamate receptor ( NMDAR ) , long implicated in developmental plasticity , shows decay time kinetics that shorten postnatally as NR2A subunits are added to the receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Transcript levels for the constitutive NMDAR subunit ( NR 1 ) and two modulatory subunits ( NR2A , NR2B ) were measured in four song control nuclei : lMAN , Area 10 , HVc , and RA . ^^^ NR 1 and NR2A mRNA levels were comparable in SD and LD groups in all four song regions studied . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Also , the NR2A / B subunits undergo activity dependent switching at synapses , indicating that their subcellular distribution may be regulated differently from the NR 1 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To test the possibility that NMDAR 2 subunits ( NR 2 ) control expression of functional NMDA receptors as well as NR 1 protein , we transferred NR2A D cDNAs into PC 12 cells using adenovirus vectors . ^^^ Prominent NMDA receptor mediated currents were recorded in PC 12 cells to which NR2A or NR2B cDNA was delivered without NR 1 cDNA . ^^^ The amplitudes of these responses were similar to those in PC 12 cells to which NR 1 cDNA was delivered together with NR2A or NR2B cDNA . ^^^ The delivery of NR2A D cDNAs also increased the amount of NR 1 protein but not that of NR 1 mRNA , suggesting that this protein increase is due to post transcriptional mechanisms . ^^^ The effects of NR2A B gene transfer on expression of NR 1 protein were much more efficient than those of NR2C D gene transfer . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The whole cell currents so produced are somewhat lower than with full length subunits , and they do not show the sag characteristic of currents from channels containing NR 1 and NR2A subunits in the continued presence of an agonist . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reactions ( RT PCR ) indicated that levels of NR 1 , NR2A and NR2D mRNAs reached peak levels between postnatal days 7 ( PND 7 ) and PND 14 . ^^^ In addition , double immunofluorescence revealed that the levels of NR 1 immunoreactivity increased but the levels of NR2A immunoreactivity were the same in parvalbumin ( PV ) positive striatal interneurons of PND 14 and adult rats . ^^^ Last but not least , in choline acetyltransferase ( ChAT ) positive striatal interneurons of PND 14 and adult rats , levels of NR 1 and NR2A immunoreactivity was seen to increase . ^^^ NR 1 , NR2A and NR2B are the major NMDA receptor subunits expressed during development . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In situ hybridization was performed for additional gene expression studies of the NR 1 , NR2A , NR2B , NR2C , and NR2D subunits . [ 3H ] MK 801 binding was found to be increased after haloperidol treatment in the striatum and nucleus accumbens . ^^^ However , the NR2A subunit was down regulated in the hippocampus and prefrontal cortex by both drugs , whereas only clozapine induced a down regulation of NR 1 in the dorsolateral prefrontal cortex . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Western blot analysis of hippocampal postsynaptic densities ( PSD ) revealed a reduction in NR2B subunit protein , whereas the abundance of the other major N methyl D aspartate ( NMDA ) receptor subunits ( NR 1 , NR2A ) , AMPA receptor subunits ( GluR2 / 3 ) , scaffolding proteins , and Ca2+ / calmodulin dependent protein kinase 2 ( alphaCaMKII ) were unaltered . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The aim of this study was to determine whether lesions of one vestibular labyrinth ( unilateral vestibular deafferentation , UVD ) result in changes in the expression of the NR 1 and NR2A subunits of the N methyl D aspartate ( NMDA ) receptor , and the GluR 2 subunit of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptor , in subregions of the rat hippocampus ( CA 1 , CA2 / 3 and the dentate gyrus ) at 10 h or 2 weeks following UVD . ^^^ No other changes in NR 1 , NR2A or GluR 2 expression were observed in any hippocampal subregion , at any time point , or in cortical tissue at any time point . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using antibodies against NR 1 , NR2A , NR2B , NR2D , GluR 2 , 3 , and GluR 5 , 6 , 7 receptor subunits we demonstrated a pronounced disturbance of cortical immunostaining pattern in the cortical malformation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To test whether NMDARs prevalent in neonates ( NR 1 plus NR2B or NR2D subunits ) are inhibited by hypoxia compared with those in mature neurons ( NR2A and NR2C ) , we expressed these receptors in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of NR 1 , NR2A , NR2C , GluR 1 , GluR6 / 7 , and KA 2 subunit proteins was not affected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Synapses in the central nucleus activate NMDA receptors that contain NR 1 and NR2B subunits , whereas synapses in the lateral nucleus contain receptors with both NR2A and NR2B subunits . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
N Methyl d aspartate ( NMDA ) receptor subunit 2A ( NR2A ) is an important modulatory component of the NMDA subtype of glutamate receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have previously shown that exposure to environmentally relevant levels of Pb ( 2+ ) during brain development decreases the expression of N methyl D aspartate receptor ( NMDAR ) subunit 1 ( NR 1 ) and NR2A genes in the hippocampus of young adult rats and was associated with deficits in hippocampal LTP and spatial learning [ Neuroscience 99 ( 2000 ) 233 242 ] . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Mutations in NR2A confirmed that the analogous region controls the pH sensitivity of this subunit and also identified the linker region between the third transmembrane domain ( M 4 ) and the S 2 portion of the NR 2 glutamate binding domain as an additional determinant of proton sensitivity . ^^^ One mutant receptor , NR 1 ( A649C ) / NR2A ( A651T ) , showed a 145 fold reduction in the IC 50 for protons ( IC 50 , 17 . 3 microM corresponding to pH 4 . 9 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
All neurones studied appeared to express the N methyl D aspartate ( NMDA ) receptor subunit NR 1 , and while antibodies recognising the NR2A and NR2B splice variants gave strong labelling , immunoreactivity with a NR2B specific antibody was weaker . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The majority of N methyl D aspartate receptors ( NMDA R ) in the adult forebrain are di or triheteromers composed of NR 1 , NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Stress and stress hormones alter the expression of mRNA for the NR 1 , NR2A and NR2B subunits of the N methyl D aspartate ( NMDA ) receptor in brain regions associated with the stress response . ^^^ Using in situ hybridization histochemistry , we measured NR 1 , NR2A and NR2B mRNA expression in the hippocampus and in the hypothalamic paraventricular nucleus ( PVN ) of rats that had undergone adrenalectomy ( ADX ) or sham surgery before or after puberty . ^^^ These results provide evidence that glucocorticoids have differential effects on the regional expression of mRNA NMDA receptor subunits and elucidate a window during adolescence in which the NR 1 , NR2A and NR2B genes are responsive to glucocorticoids . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using preembedding and postembedding immunostaining , as well as electron microscopy , we quantified the volumetric densities of NR 1 , NR2A , and NR2B containing synapses in layers 4 and 2 / 3 of the ferret visual cortex at different postnatal ages . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The distributions of NR2A and NR2C showed temporal and spatial similarities to that of NR 1 , while the expression of NR2B showed differences in the intensity and distribution . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Glutamate receptors AMPA GluR2 / 3 and NMDA R2A : NR2A ( NMDAepsilon 1 ) , and GABA ( A ) ( GABA ( A ) Ralpha 1 ) receptors were examined by immunohistochemistry in the cerebral cortex ( frontal cortex ) entorhinal cortex , hippocampus and cerebellar cortex in nine patients with sporadic Creutzfeldt Jakob disease ( CJD ) and eight age matched controls obtained 3 8 h after death . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The analysis revealed significant changes in genes associated with the NMDA receptor / postsynaptic density complex and the vesicle exocytosis machinery including NMDA receptor NR 1 and NR2A subunits , densin 180 , brain enriched guanylate kinase associated protein , synaptosome associated protein of 25 kDa , synaphin / complexin and vesicle associated membrane protein 2 / synaptobrevin 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Since NR 2 subunits are not transported to the cell surface unless they associate with NR 1 subunits , cell surface expression of NR2A can be used to monitor the association of the different subunits in cells transfected with N and C terminally truncated NR 1 subunits . ^^^ By combining measurements of cell surface expression of NR2A with co immunoprecipitation experiments , and by using Blue Native gel electrophoresis to determine the oligomerization status of the subunits , we have shown that regions of the N terminus of NR 1 are critical for subunit association , whereas the truncation of the C terminus of NR 1 before the last transmembrane region has no effect on the association of the subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the cerebral cortex of flurothyl treated rats , the protein expressions of NR 1 , NR2A , and NR2B decreased significantly , but NR2C increased significantly . ^^^ In the hippocampus of flurothyltreated rats , the protein expression of NR2A decreased significantly , the protein expression of NR2C increased significantly , and NR 1 and NR2B were similar to that in the hippocampus of control group . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Intravenous injection of GF immediately following the ischemic challenge resulted in decreased phosphorylation of NR 1 on PKC phosphorylation sites and reduced ischemia induced increases in tyrosine phosphorylation of NR2A and NR2B without affecting the increase in total tyrosine phosphorylation of hippocampal proteins . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression of NMDA receptor NR 1 , NR2A and NR2B subunit mRNAs during development of the human hippocampal formation . ^^^ We used in situ hybridization to examine the distribution and relative abundance of NR 1 , NR2A and NR2B subunit messenger ribonucleic acids in the hippocampal formation and adjacent cortex of 34 human subjects at five stages of life ( neonate , infant , adolescent , young adult and adult ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The activity of the N methyl D aspartate ( NMDA ) receptor , a subclass of ionotropic glutamate receptor , is modulated by a complex network of phosphorylation and dephosphorylation . 1 investigated the relative extent of tyrosine phosphorylation of NMDA receptor subunit 2A ( NR2A ) and 2B ( NR2B ) subunits in the rat forebrain postsynaptic density ( PSD ) fraction . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Laminar distribution of NMDA receptor subunit ( NR 1 , NR2A , NR2B ) expression during the critical period in cat visual cortex . ^^^ The present study used immunohistochemistry to determine developmental changes in the laminar distribution of the three major cortical NMDA subunits ( NR2A , NR2B , NR 1 ) during the critical period in cat visual cortex . ^^^ NR 1 showed a developmental pattern of expression different from NR2A and NR2B after 1 week . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Coimmunoprecipitation and electrophysiological analyses demonstrated that NR3B , when exogenously introduced into hippocampal neurons , can coassemble with endogenous NR 1 and NR2A and can reduce the Ca2+ permeability of NMDA currents . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
As expected , currents recorded from cells expressing the NR 1 subunit and a zinc insensitive NR2A mutant ( H128S ) were not augmented by zinc chelators . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we show that NR2B , but not NR2A or NR 1 subunits of the NMDAR , interacts in vivo and in vitro with RasGRF 1 , a Ca ( 2+ ) / calmodulin dependent Ras guanine nucleotide releasing factor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Proteins were transblotted and probed with NMDA receptor subunit 1 ( NR 1 ) , NR2A or NR2B antibodies . ^^^ As tissue PCr levels decreased from 3 . 5 to 0 . 5 micromol / g brain during hypoxia , NR 1 , NR2A and NR2B protein increased by 84 , 56 and 38 % , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 and NR2A fusion proteins formed functional , excitotoxic channels upon co expression . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Cultures treated for up to 5 weeks with epidermal growth factor , fibroblast growth factor 2 , retinoic acid , and nerve growth factor displayed neuron like cellular processes and expressed neuronal markers , including the neuronal nuclear antigen NeuN , microtubule associated protein 2 , tau , synaptophysin , alpha ( 1A ) and alpha ( 1B ) calcium channel subunits , NR2A glutamate receptor subunits , and gamma aminobutyric acid . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At hippocampal CA 3 to CA 1 synapses of adult mice , different NMDAR subtypes with distinct functionality assemble from NR 1 with NR2A and / or NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Neuron density , the expressions of N methyl D aspartate ( NMDA ) receptor subunit proteins ( NR 1 , NR2A , NA2B ) and vascular cell adhesion molecule 1 ( VCAM 1 ) in the cerebral cortex and hippocampus were measured at 72 h after reperfusion . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Although examining NMDA receptor subunit proteins showed no change in NR 1 , NR2A , or NR2B in the striata of the symptomatic mice , we observed a decrease in phosphorylation of NR 1 at Ser 897 , previously reported to decrease NMDA receptor current . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In striatal neurons , NR 1 , NR2A and NR2B mRNAs and immunoreactivity , and NR2D mRNA were found and the maximal levels of NR 1 mRNA and immunoreactivity expression were found at 6 day in vitro ( DIV ) . ^^^ NMDA receptors found at this stage in striatal neurons are likely to contain NR 1 plus NR2A , NR2B and NR2D subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
R6 / 2 mice had decreased levels of mRNAs encoding epsilon 1 and epsilon 2 NMDA receptor subunits ( mouse orthologs of rat NR2A and NR2B subunits ) , but not the zeta 1 subunit ( mouse ortholog of NR 1 ) , as assessed by gene expression profiling and Northern blotting . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Neuronal injury and expression of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) mRNA were detected by using in situ staining and RT PCR , respectively . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Activity dependent changes in expression and localization of the largest major isoform of the neural cell adhesion molecule NCAM 180 and three subtypes of glutamate receptors predominantly expressed in the outer part of the molecular layer of the dentate gyrus of adult rats the NMDA receptor NR2A , the AMPA receptor GluR2 / 3 , and the metabotropic glutamate receptor mGluR 1 were investigated using postembedding immunogold labeling , and electron microscopy . ^^^ Thus , changes in synaptic strength are associated with concomitant changes in the expression and distribution of NCAM 180 and glutamate receptors , particularly of the NR2A subtype . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Surface expression of NMDA receptors composed of NR 1 subunit and NR2A subunit mutants with partially deleted C terminus in HEK 293 cells ] . ^^^ CONCLUSION : Within the region downstream from the 897L of NR2A subunit , neither a particular domain directly interacted with ER retention domain in NR 1 1a C 1 cassette , nor that determining ER retention of NR2A subunit itself has been found , indicating that more complicated mechanisms might exist in which the subunit assembling and targeting to plasma membrane of NMDA receptors undergo . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In striatal tissue from DARPP 32 depleted mice , basal tyrosine and serine phosphorylation of striatal NMDA receptor subunits NR 1 , NR2A , and NR2B was normal , and activation of dopamine D 1 receptors with the agonist SKF 82958 [ ( + / ) 6 chloro 7 , 8 dihydroxy 3 allyl 1 phenyl 2 , 3 , 4 , 5 tetra hydro 1H benzazepine ] produced redistribution of NMDA receptors from vesicular compartments ( P 3 and LP 2 ) to synaptosomal membranes ( LP 1 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the absence of extracellular calcium , substitution of Arg for Asn 598 ( NR1R ) slowed desensitization by 2 to 3 fold compared with wild type NR1 / NR2A , and glutamate evoked peak current EC 50 and deactivation rate were also affected . ^^^ NR 1 is an obligate subunit in all NMDAR complexes , where it combines with NR2A , 2B , 2C , and / or 2D . ^^^ Here , we report that mutations of NR 1 ( Asn 598 ) in combination with wild type NR2A , expressed in human embryonic kidney 293 cells , exhibit altered glycine independent desensitization . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 2a ( c Myc ) / NR2A receptors when expressed in mammalian cells are not trafficked to the cell surface nor do they yield cell cytotoxicity post transfection . ^^^ NR 1 2a ( c Myc ) was , however , shown to assemble with NR2A subunits by immunoprecipitation and [ ( 3 ) H ] MK 801 radioligand binding assays . ^^^ Immunoblots of cells co transfected with wild type NR 1 2a / NR2A subunits yielded two NR 1 2a immunoreactive species with molecular masses of 115 and 226 kDa . ^^^ Only the 115 kDa NR 1 2a species was detected for NR 1 2a ( c Myc ) / NR2A . ^^^ A series of cysteine > alanine NR 1 2a mutants was generated , and the NR 1 2a mutants were co expressed with NR2A or NR2B subunits in mammalian cells and characterized with respect to cell surface expression , cell cytotoxicity post transfection , co association by immunoprecipitation , and immunoblotting following SDS PAGE under both reducing and non reducing conditions . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus , we investigated the expression of the NR 1 , NR2A , and NR2B subunits after combined prenatal and postnatal exposure to EtOH . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using quantitative Western blotting , the levels of NR 1 , NR2A and NR2B subunits were measured in the cortex and hippocampus of rats at different times after PTZ injection . ^^^ In the early seizure developmental process , both NR 1 and NR2A were markedly increased in the cortex , and NR 1 was significantly increased in the hippocampus . ^^^ On the other hand , in the kindled process both NR 1 and NR2A decreased in the cortex and hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Receptors containing NR 1 and NR2A ( V820W ) or NR2A ( M817W ) mutant subunits expressed in HEK 293 cells were not functional . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To address these contradictory results , NMDA receptor mediated currents were recorded from HEK 293 cells transfected with NR 1 and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemical analysis confirmed expression of NR 1 , NR2A , and NR2B subunits in neurospheres cultured for 4 12 DIV . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The levels of cortical NR 1 NR2A and NR2B subunit proteins were detected by immunoblotting . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Acutely after injury significant decreases were observed in the GluR 1 , GluR 2 , and GluR 4 subunits of the alpha amino 3 hydroxy 5 methyl 4 isoxazole proprionate ( AMPA ) receptor , and the NR2A and NR2B subunits , but not the NR 1 subunit , of the N methyl d aspartate ( NMDA ) receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using immunohistochemistry and RTPCR normal and OA chondrocytes are shown to express NR 1 and NR2a subunits of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study , we identify a conserved family of membrane proximal endocytic signals , two within the NMDAR type 1 ( NR 1 ) subunit and one within the NR2A and NR2B subunits , necessary and sufficient to drive the internalization of NMDARs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
However , this decrease was not the same for both subunits , resulting in a decrease ( over 30 % ) in the NR2A : NR2B ratio indicating that the NMDAR had temporarily become more sensitive to glutamate and would remain open longer once activated . ^^^ To test this hypothesis , adult rats were subjected to a lateral fluid percussion brain injury and the NR 1 , NR2A and NR2B subunits measured within different regions . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Compared with the control rats , the protein expressions of NR 1 , NR2A and NR2B in the cerebral cortex and NR2A in the hippocampus of the recurrent seizure group was significantly decreased , but NR2C protein expression in the cerebral cortex and hippocampus significantly increased . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The aim of this study was to investigate whether L Asp and NMDA inhibit histamine stimulated GMBF and to examine the expression patterns of NMDA receptor subunits NR 1 , NR2A , and NR2B in rat stomach . ^^^ In addition , the distribution patterns of NR 1 , NR2A , and NR2B contaning NMDA receptors in rat stomach were determined immunohistochemically by using specific antibodies against NR 1 , NR2A , and NR2B . ^^^ The fact that NMDA receptor subunits NR 1 , NR2A , and NR2B were found to be localized in the rat stomach as visualized immunohistochemically with specific antibodies against NR 1 , NR2A , and NR2B is consistent with this hypothesis . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We have compared the expression of N methyl d aspartate ( NMDA ) receptor subunits ( NR 1 , NR2A D ) , NR 1 splice variants ( NR 1 1a / 1b , 2a / 2b , 3a / 3b , 4a / 4b ) , and the neuronal and inducible isoforms of NO synthase ( nNOS and iNOS ) in the RVLM of Wistar Kyoto ( WKY ) and spontaneously hypertensive rats ( SHR ) , based on the hypothesis that altered NMDA receptor make up or altered expression of endogenous NO may be associated with the increase in sympathetic output described from this site in hypertension . ^^^ Quantitative real time PCR indicated that for both strains of rat , mRNA for the NR 1 subunit ( all splice variants ) was the most abundant ( 16 . 5 fold greater , P < or =0 . 05 , relative to the NR2A subunit ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Mutations of several residues in the lurcher motif of either NR 1 or NR2A significantly reduce proton sensitivity of recombinant NR1 / NR2A receptors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptor channel block by Mg ( 2+ ) ( o ) depends on receptor subunit composition : NR1 / 2A receptors ( receptors composed of NR 1 and NR2A subunits ) and NR1 / 2B receptors are more strongly inhibited by Mg ( 2+ ) ( o ) than are NR1 / 2C or NR1 / 2D receptors . ^^^ The k ( + , app ) for NR1 / 2D receptors was similar to but moderately slower than the k ( + , app ) obtained from cortical NMDA receptors composed of NR 1 , NR2A and NR2B subunits at all voltages tested . ( 3 ) Mg ( 2+ ) ( o ) blocking events induced an additional component in the closed duration distribution ; this component was used to estimate the apparent unblocking rate of Mg ( 2+ ) ( o ) ( k ( , app ) ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Since the normal function of NMDA receptor is critical for synaptogenesis , the long term effects of toluene exposure during synaptogenesis on the neurobehavioral function and the expression of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) were examined . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblot analysis revealed that prolonged toluene exposure increased levels of NR2A and NR2B NMDA receptor subunits with no change in NR 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR2A cleavage by calpain occurred in the cell surface and intracellular fractions and required the presence of NR 1 subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We studied the distribution pattern of different subunits of the NMDA receptor within the rat retina including quantifying the pattern of labelling for all the NRI splice variants , the NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Synaptic distribution of the NR 1 , NR2A and NR2B subunits of the N methyl d aspartate receptor in the rat lumbar spinal cord revealed with an antigen unmasking technique . ^^^ We have used an antigen unmasking method involving pepsin treatment to reveal NR 1 , NR2A and NR2B subunits and have compared their distribution with that of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid receptor GluR 2 subunit , which is thought to be present at most glutamatergic synapses throughout the spinal cord . ^^^ Although NR 1 puncta were present throughout the grey matter , NR2A was concentrated in laminae 3 4 and NR2B in laminae 1 2 . ^^^ The majority of puncta labelled with each NMDA receptor antibody were GluR 2 immunoreactive , which suggests that they were present at synapses , and this was confirmed with electron microscopy for the NR 1 and NR2A antibodies . ^^^ In laminae 1 2 , most NR2B puncta were also NR 1 immunoreactive and a similar arrangement was found for NR2A / NR1 in laminae 3 4 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At the light microscopic level , both NR2A and NR2B exhibit a distribution in monkey and rat hippocampus very similar to NMDA receptor subunit NR 1 , and both are highly colocalized with NR 1 . ^^^ Electron microscopic immunogold studies demonstrated that both NR2A and NR2B are often present in asymmetric synapses in CA 1 , commonly colocalized with NR 1 , and often colocalized with each other in the same asymmetric synapses . ^^^ The confocal and ultrastructural data suggest that whereas NR 1 , NR2A , and NR2B are essentially uniformly colocalized in hippocampal projection neurons , there is extensive heterogeneity at the synaptic level that would lead to multiple functional classes of NMDA receptor mediated synapses , and extensive capacity for plasticity at the synapse . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunocytochemistry revealed that dendrites and somas displayed distinct NR 1 and NR2A subunit clusters which became increasingly colocalized in + / + neurones . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Recordings from outside out patches that contain a single active channel show that NR2A containing receptors have a higher probability of opening at least once in response to a brief synaptic like pulse of glutamate than NR2B containing receptors ( NR2A , 0 . 80 ; NR2B , 0 . 56 ) , a higher peak open probability ( NR2A , 0 . 50 ; NR2B , 0 . 12 ) , and a higher open probability within an activation ( NR2A , 0 . 67 ; NR2B , 0 . 37 ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
On the other hand , several residues in the lurcher motif of either NR 1 or NR2A are critical for the glycine independent desensitization of NR1 / NR2A receptors . ^^^ Since all these mutations also alter the proton sensitivity or deactivation time constants of NR1 / NR2A receptors , our data suggest that the channel gating and desensitization of NMDA receptors share common molecular determinants and that the lurcher motif of NR 1 and NR2A is critical for both processes . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Myosin RLC co localized with NR 1 in the dendritic spines of isolated hippocampal neurons , and was co immunoprecipitated from brain extracts in a complex with NR 1 , NR2A , NR2B , PSD 95 , Adaptor protein 2 and myosin 2 heavy chain . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
After 3 weeks in culture ( days in vitro [ DIV ] 22 ) , the numbers of postsynaptic receptor clusters with N 1 containing NR 1 splice variants and NR2A subunits are upregulated . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The expression of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) at 5 14 days in vitro ( DIV ) were also determined . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The present study characterized the long term effects of toluene exposure during synaptogenesis on the expression of NMDA receptor subunits ( NR 1 , NR2A and NR2B ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Structural features of the glutamate binding site in recombinant NR1 / NR2A N methyl D aspartate receptors determined by site directed mutagenesis and molecular modeling . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunohistochemical studies verified that N methyl D aspartate ( NMDA ) receptor subunit proteins NR 1 and NR2A / 2B are expressed in the medial parvocellular PVN , indicating the potential for NMDA receptor regulation of corticotropin releasing hormone ( CRH ) release . ^^^ No changes were observed for NR 1 or NR2A mRNAs . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RESULTS : Our data confirm the mRNA expression of the NR 1 , NR2A , NR2B and NR2D subunits of NMDA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Quantitative immunoblotting was used to determine the subcellular abundance of NR 1 , NR2A and NR2B subunits in striata from unlesioned , MPTP lesioned ( parkinsonian ) and MPTP lesioned , levodopa treated ( dyskinetic ) macaques . ^^^ In parkinsonian macaques , NR 1 and NR2B subunits in synaptosomal membranes were decreased to 66 + / 11 % and 51 . 2 + / 5 % of unlesioned levels respectively , while the abundance of NR2A was unaltered . ^^^ Levodopa treatment eliciting dyskinesia normalized NR 1 and NR2B and increased NR2A subunits to 150 + / 12 % of unlesioned levels . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NMDA receptors ( NMDAR ) are heteromers associating the NR 1 subunit and one of the four types of NR 2 subunits ( NR2A to D ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Following ischemia , the phosphorylation of NR 1 by protein kinase C and tyrosine phosphorylation of NR2A and NR2B was increased in both lipid rafts and PSDs , with a greater increase in tyrosine phosphorylation occurring in the raft fraction . ^^^ Following ischemia , NR 1 , NR2A and NR2B levels were elevated in PSDs and reduced in lipid rafts . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunoblotting analysis showed that the NR2A and NR2B subunits of NMDARs were markedly degraded in cultured cortical neurons treated with glutamate , and the degradation of NR 2 subunits was prevented by calpain inhibitors . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using excitotoxicity as a proxy for exaggerated NMDAR activity , we analyzed the effect of over expressing NR 1 4 and NR2A C terminal domains on exaggerated NMDAR function . ^^^ We demonstrate that a determinant within the C terminal domain of NR 1 4 ( C 02 ' ) is important for NMDAR excitotoxicity , whereas no novel determinants were identified in the NR2A C terminus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 , NR2A or NR2B antisense ODNs were incubated alone or with PCP for 48h . ^^^ One day following washout , it was observed that PCP treatment caused an increase in NR 1 , NR2A and Bax polypeptides in the cortex , but had no effect on Bcl xL . ^^^ Co incubation of PCP with either NR 1 or NR2A antisense significantly reduced PCP induced apoptosis , while neither NR2B antisense ODN nor NR 1 sense ODN used as a control had an effect . ^^^ That is , western analysis showed that antisense ODNs directed against either NR 1 or NR2A prevented PCP induced increases in Bax in addition to preventing the upregulation of the respective receptor proteins . ^^^ These data suggest that NR 1 and NR2A antisense ODNs offer neuroprotection from apoptosis , and that upregulation of the NR 1 and NR2A subunits following PCP administration is at least partly responsible for the observed apoptotic DNA fragmentation . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
To test this hypothesis further , we examined the effects of 10 daily s . c . injections of IGF 1 on NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) , GH receptor ( GHR ) , GH binding protein ( GHBP ) and type 1 IGF receptor ( IGF 1R ) gene transcripts in the hippocampus . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Using coimmunoprecipitation , Western blotting , and mass spectrometry , we show that inhibition of tyrosine phosphatases triggers disassembly of NR 1 , NR2A , and NR2B in cortical NMDA receptor complexes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
MK 801 not only decreased the expression of the NR 1 subunit of NMDA receptors but also increased NR2A expression , without affecting NR2B expression . ^^^ However , the repetitive magnetism prevented decreases in both BDNF mRNA and MAP 2 and additionally increased the expression of NR2A subunit , without altering NR 1 expression in neurons cultured in the presence of MK 801 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Because structural heterogeneity might be the basis for physiological properties , we examined the expression of six NMDA receptor subunits ( NMDAR 1 , NR2A 2D , and NR3A ) plus eight NMDR 1 splice variants in pre Botzinger complex , hypoglossal and , for comparison , neurons from the nucleus of the solitary tract in young rats using single cell multiplex RT PCR . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
METHODS : NR 1 and NR2A , two NMDAr subunits , were probed in rat carotid arteries by immunohistochemistry . ^^^ RESULTS : Both NR 1 and NR2A were expressed in rat carotid arteries . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The results showed that NR 1 , NR2A , and NR2B subunits could form homodimers when they were expressed alone in HEK 293 cells . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast to NRI and NR2C , very weak immunoreactivity of NR2A and NR2B subunits was observed in the LC . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
During the same period , neurochemical investigations have shown that some hippocampal subregions exhibit long term changes in the expression of neuronal nitric oxide synthase , arginase 1 and 2 , and the NR 1 and NR2A N methyl D aspartate ( NMDA ) receptor subunits following peripheral vestibular damage . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At CBF 30 > 9 min , we found downregulation of mRNA for NR2A , NR2B , and NR3B at 3 5 h after SAH , whereas the levels of NR 1 mRNA were unaffected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Basolateral and central amygdala tissues were assayed for protein expression of the alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) receptor subunits ( GluR 1 and GluR 2 ) and the NMDA receptor subunits ( NR 1 , NR2A and NR2B ) . ^^^ In the central amygdala , GluR 2 but not GluR 1 levels were increased on days 1 and 30 , NR 1 levels were increased on day 30 and NR2A or NR2B levels were not altered after withdrawal from cocaine . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here we report that covalent modification of NR 1 A652C or the analogous mutation in NR2A , 2B , 2C , or 2D by methanethiosulfonate ethylammonium ( MT SEA ) occurs only in the presence of glutamate and glycine , and that modification potentiates recombinant NMDA receptor currents . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
At PN 2 , expression of whole brain NMDA receptor subunits NR 1 , NR2A , and NR2B was lower in pups from dams fed ZD and MZD compared to controls , as analyzed using relative RT PCR and immunoblotting . ^^^ At PN 11 , whole brain and hippocampi NR 1 , NR2A , NR2B and PSA NCAM ( polysialic acid neural cell adhesion molecule ) expression and the number of PSA NCAM immunoreactive cells were lower in pups from dams fed ZD compared to controls . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The ethanol sensitive NMDA receptor subunits NR 1 , NR2A and NR2B were quantified by Western immunoblot analysis . ^^^ Exposure to ethanol ( 50 mM ) caused an increase in the levels of NR 1 ( 137 + / 11 % of untreated control , P = 0 . 009 ) , NR2A ( 128 + / 14 % , P = 0 . 022 ) and NR2B ( 136 + / 19 % , P = 0 . 012 ) . ^^^ Coincubation with memantine ( 10 microM ) completely blocked the ethanol induced up regulation of NR 1 ( 102 + / 4 % ) , NR2A ( 95 + / 7 % ) and NR2B ( 105 + / 13 % ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Expression levels of NR 1 , NR2A , but not NR2C , also tended to be higher in ArKO mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 , NR2A and NR2C subunits expression after cervical spinal cord transplant and section in dogs . ^^^ This paper served to evaluate the expression levels of subunits NR 1 , NR2A and NR2C which are implicated in neuronal plasticity events . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Here , we show that in transgenic mice overexpressing the human AD gene APPswe ( Tg 2576 ) , safflower oil induced n 3 PFA deficiency caused a decrease in N methyl D aspartate ( NMDA ) receptor subunits , NR2A and NR2B , in the cortex and hippocampus with no loss of the presynaptic markers , synaptophysin and synaptosomal associated protein 25 ( SNAP 25 ) . n 3 PFA depletion also decreased the NR 1 subunit in the hippocampus and Ca2+ / calmodulin dependent protein kinase ( CaMKII ) in the cortex of Tg 2576 mice . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Thus , we report two NMDAR 1 genes ( NR1 . 1 and NR1 . 2 ) , and eight NMDAR 2 genes , designated NR2A . 1 and NR2A . 2 , NR2B . 1 and NR2B . 2 , NR2C . 1 and NR2C . 2 , and NR2D . 1 and NR2D . 2 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
An analysis of NR 1 and NR 2 protein expression demonstrated no change in the levels of NR 1 splice variants or NR2A following the inflammation . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We studied adsorption capacity of 5 latexes to synthetic peptide fragments of mu and delta opioid receptors and to GluR 1 and NR2A subunits of glutamate receptor . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
There are at least eight splicing variant isoforms of the NR 1 subunit and four types of NR 2 subunits : NR2A , NR2B , NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Secondly , we used immunoblotting and immunohistochemistry to investigate the effects of nerve injury on NMDA receptor subunit expression , revealing increased expression of NR2B , but not NR2A and reduced NR 1 in the superficial dorsal horn . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Allosteric interaction between zinc and glutamate binding domains on NR2A causes desensitization of NMDA receptors . ^^^ Previous work suggests that fast desensitization of NR1 / NR2A receptors is caused by ambient zinc , and that a positive allosteric interaction occurs between the extracellular zinc binding amino terminal domain and the glutamate binding domain of NR2A . ^^^ Here we demonstrate that this allosteric coupling reflects interactions within the NR2A subunit , and that the affinity of zinc for its binding site is regulated by glutamate binding and not by glycine binding nor by channel pore opening . ^^^ We subsequently use this model to evaluate the effects of extracellular zinc on NR1 / NR2A excitatory postsynaptic currents ( EPSCs ) by simulating the response to a brief synaptic like pulse of glutamate . ^^^ Modelling results show that zinc at a steady state concentration of at least 100 nm has a significant effect on the amplitude of NMDA EPSCs but that concurrent release of 10 microm zinc with synaptic glutamate release has little effect on the amplitude of a single NR1 / NR2A NMDA EPSC . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The same compounds were preliminarily assayed using Xenopus oocytes expressing cloned rat NMDA receptors containing the NR 1 subunit in combination with either NR2A , NR2B , NR2C , or NR2D subunit . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Aliquots of NR 1 , NR2A and NR2B proteins from cell homogenate were immunoprecipitated with protein A agarose and detected by Western blotting . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Within the nigrostriatal pathway , NR 1 , NR2A , NR2B , GluR 1 , GluR6 / 7 and KA 2 were increased in the dorsal CPu , whereas no significant changes were observed in the SN . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RT PCR analysis showed that human resting peripheral blood lymphocytes ( PBL ) and Jurkat T cells express genes encoding for both NR 1 and NR2B subunits : phytohemagglutinin ( PHA ) activated PBL also expresses both these genes and the NR2A and NR2D genes . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In surviving neurons , multiple changes occurred in the proportion of individual NMDA receptor subunit 1 ( NR 1 ) splice variant mRNA expression , whereas the overall expression of NR 1 , NR2A and NR2B transcripts remained unaffected . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A subunits showed clustered expression in cell processes , but NR3B was absent . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The NR2A pre M 1 region , the linker between the glutamate binding domain and the channel pore , plays a critical role in desensitization . ^^^ The rate of MTSET modification of the homologous residue on NR 1 ( NR 1 1a ( L562C ) / NR2A ) was much slower than V557C . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Protein kinase C ( PKC ) phosphorylates the NR 1 and NR2A subunits of NMDARs at consensus sites located within their intracellular C terminal tails . ^^^ When co expressing NR 1 ( stop 838 ) / NR2A the effects of PMA could only be observed with agonist concentrations sufficient to induce glycine insensitive desensitization . ^^^ We conclude that activation of PKC increases NMDAR glycine insensitive desensitization independently of previously identified sites located within the NR 1 C terminus and distal segment of the NR2A C terminus . . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In this study we examined the effects of chronic morphine administration on gene and protein expression of three major NMDA receptors subunits ( NR 1 , NR2A , and NR2B ) in NAcc and CeA . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Compared to the contralateral side , ipsilateral basal ganglia NR 1 and NR2A subunit expression transiently increased at 1 hour after ICH and thrombin injection . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
We did not detect any differences in immunoreactivity in any of the non phosphorylated NR 1 , NR2A , NR2B , NR2C or the NR2D subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Mutant NR 1 subunits were coexpressed with the NR2A subunit in HEK 293 cells and examined for alterations in ethanol sensitivity using whole cell voltage clamp electrophysiology . ^^^ RESULTS : Replacing phenylalanine at TM 3 position 639 in the NR 1 subunit ( F 639 ) with 9 different amino acids produced functional receptors when coexpressed with the NR2A subunit . ^^^ Substitution of tryptophan for TM 4 residues in the NR 1 subunit ( positions 820 822 ) that are homologous to a site in the NR2A subunit shown to reduce ethanol inhibition ( A825W ) had no effect on ethanol sensitivity . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Furthermore , the association between PSD 95 and NMDAR subunits ( NR 1 , NR2A , and NR2B ) in the hippocampal CA 1 was also markedly altered by perinatal hypoxia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Recently , it was shown that ethanol inhibition of NMDA receptors was reduced by mutation of residues in the transmembrane ( TM ) segment 3 of the NR 1 subunit ( F639A ) or in TM 4 of the NR2A subunit ( A825W ) , suggesting putative ethanol binding sites . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
According to the quantitative changes of NMDA receptor subunits , including NR 1 , NR2A , and NR2B , induced by diverse types of reinforcers , we chose NR2B subunit as the target of research . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Hippocampal mRNA expression of the NMDA subunits NR 1 , NR2A and NR2B , insulin like growth factor type 1 receptor ( IGF 1R ) , and postsynaptic density protein 95 ( PSD 95 ) was then measured in the animals by Northern blot analysis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Immunohistochemistry demonstrated that the NT 2 neurons are mostly GABAergic ; they predominantly express the NMDA receptor subunits NR2B and NR2C , and lower levels of NR 1 and , particularly , of NR2A . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Ammonia reduces MK 801 binding to NMDA receptors and the surface expression of the NR 1 and NR2A subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study we used RT PCR methods on single cells and tissue to compare the expression of NMDAR subunits , NR 1 , NR2A and NR2B , in the striatum of R6 / 2 transgenic mice with their wild type ( WT ) littermates at three different age groups corresponding to different symptomatic milestones ( 19 25 days showing no overt evidence of abnormal behavior , 38 45 days at the onset of the overt phenotype and 78 90 days displaying the full behavioral phenotype ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Mutation of Ser 670 to glycine ( S670G ) in NR2A reduced the potency of glutamate by 124 fold . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In contrast , the abundance of other alpha 2 3 , and beta glycine receptor , gephyrin , the anchoring protein of glycine receptor , the alpha 1 , beta 2 and gamma 2 subunits of GABAA receptors , GluR 2 , R 3 subunits of alpha amino 3 hydroxi 5 methylisoxazole 4 propionic acid receptors , and NR 1 and NR2A transcripts of N methyl D aspartate receptors was unaffected during the first week following the lesion . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
In the present study , we performed western blot analysis to determine whether protein levels of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) and associated PSD proteins ( NF L , PSD 95 , SAP 102 ) are altered in schizophrenia . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
RT PCR analysis showed no significant changes in NR 1 , NR2A , or NR2B gene expression ; however , as a positive control , hsp 70 was induced more than twofold in ipsilateral cortex and hippocampus on PID 1 . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
The IC 50 values for NVP AAM 077 acting at NR1 / NR2A NMDA receptors were , as expected , dependent on the glutamate concentration used to evoke responses , being 31 + / 2 nM ( with glutamate at its EC 50 concentration ) and 214 + / 10 nM ( at 10 times the EC 50 concentration ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
METHODS : The effects of chronic antipsychotic drug administration ( haloperidol and clozapine ) on protein levels of NR 1 , NR2A and NR2B proteins were evaluated in the nucleus accumbens ( NAc ) , putamen ( PUT ) , dorsolateral prefrontal cortex ( DLPFC ) , superior temporal gyrus ( STG ) , and entorhinal cortex ( EC ) of rhesus monkeys using Western blot analysis . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Double immunostaining for HDC and NMDA receptor subunits showed that histamine neurons of the rat tuberomammillary nucleus express NMDA receptor subunit 1 ( NR 1 ) with NMDA receptor subunit 2A ( NR2A ) and NMDA receptor 2B subunit ( NR2B ) . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Also , the expression level of NMDA receptors 1 ( NR 1 ) , NMDA receptors 2A ( NR2A ) , NMDA receptors 2B ( NR2B ) and AMPA ( GluR 1 subunit ) were determined by grayscale analysis in immunohistochemical stained slices . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
Previous work showed that permeant ions have profound effects on Mg2+ ( o ) block of NMDA receptors composed of NR 1 , NR2A , and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q05586	Pubmed	SVM Score :0.0
NA