| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| With the approval of alteplase ( tPA ) therapy for stroke , it is likely that combination therapy with tPA to restore blood flow , and agents like glutamate receptor antagonists to halt or reverse the cascade of neuronal damage , will dominate the future of stroke care . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| When confluent cultures of HUVECs ( passages 2 to 3 ) were conditioned with purified notoginsenoside R 1 ( NR 1 ) , a dose ( 0 . 01 to 100 micrograms NR1 / mL ) and time dependent increase in tissue type plasminogen activator ( TPA ) synthesis was observed , which was significant from 0 . 1 microgram NR1 / mL and from 6 hours of incubation with 100 micrograms NR1 / mL on . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Arginine 260 of the amino terminal domain of NR 1 subunit is critical for tissue type plasminogen activator mediated enhancement of N methyl D aspartate receptor signaling . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Besides its well established role in wound healing and fibrinolysis , tissue type plasminogen activator ( t PA ) has been shown to contribute to cognitive processes and memory formation within the central nervous system , and to promote glutamate receptor mediated excitotoxicity . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The effects of 12 O tetradecanoylphorbol 13 acetate ( TPA ) , a potent activator of protein kinase C , on high affinity Na ( + ) dependent glutamate transport were investigated in primary cultures of neurons and glial cells from rat brain cortex . ^^^ Incubation of glial cells with TPA led to concentration and time dependent increases in the glutamate transport that could be completely suppressed by the addition of the protein kinase C ( PKC ) inhibitor 1 ( 5 isoquinolinylsulfonyl ) 2 methylpiperazine . ^^^ Under the chosen experimental conditions TPA had no effect on glutamate transport in neurons . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The activities of the microsomal marker , NADPH cytochrome c reductase and the mitochondrial marker , glutamate dehydrogenase were not enhanced by TPA treatment . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In fact , TPA induced only a small increase in the KD but no change in the Bmax of [ 3H ] glutamate binding in cerebellar membranes . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Treatment of hippocampal neurons with glutamate / glycine ( Glu / Gly ) , ionomycin , or 12 O tetradecanoylphorbol 13 acetate ( TPA ) increased 32P labeling of immunoprecipitated alpha amino 3 hydroxy 5 methyl 4 isoxazoleproprionate ( AMPA ) type GluRs by 145 % , 180 % , and 227 % , respectively , of control values . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Exposure of cells to either PGF 2 alpha or TPA completely inhibited the low pH induced increases in ( 15N ) ammonia formation from incubations with 5 15N , reflecting reduced flux through the mitochondrial phosphate dependent glutaminase , and from ( 2 15N ) glutamine , reflecting reduced flux through the mitochondrial glutamate dehydrogenase pathway . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Glutamate transport studies were performed in confluent human umbilical vein endothelial cells which were treated with the phorbol ester 12 myristate 13 acetate ( TPA , 0 1000 nM ) , a compound which directly activates PKC . ^^^ TPA inhibited Na ( + ) independent System xAG glutamate transport by 70 % but only slightly reduced Na ( + ) dependent activity . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| We examined the effect of basic fibroblast growth factor ( bFGF ) on the survival of cultured rat hippocampal neurons exposed to conditions in which PKC is likely to play a role . bFGF reduced neuron damage caused by the PKC activating phorbol ester 12 O tetradecanoylphorbol 13 acetate ( TPA ) , glutamate and ischaemia like culture conditions . bFGF was able to counteract the excessive activation of PKC caused by these treatments . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| A neuronal cell line , HT 22 , is sensitive to glutamate cytotoxicity via a non receptor mediated oxidative pathway . 12 O tetradecanoylphorbol 13 acetate ( TPA ) , an activator of protein kinase C , blocks this glutamate induced cell death . ^^^ Down regulation of protein kinase C eliminates the protection against glutamate cytotoxicity afforded by TPA . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with pertussis toxin ( PTX ) for 24 h or with 12 O tetradecanoylphorbol 13 acetate ( TPA ) for 30 45 min prior to NE addition abolished the NE mediated effect on glutamate uptake . ^^^ Addition of TPA alone resulted in a rapid increase of glutamate uptake , which declined to control levels when TPA was applied 30 min prior to uptake initiation by glutamate . ^^^ The increase in glutamate uptake elicited by TPA and NE added at the same time showed no additivity of the stimulatory effect resulting from treatment with each agent alone . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The effects of phorbol 12 tetradecanoyl 13 acetate ( TPA ) and dibutyryl cAMP on the glutamate transport present in chick Bergmann glial cell ( BGC ) cultures were examined . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Exposure of granule cells in culture to forskolin results in secretion of tPA , elongation of mossy fiber axons , and formation of new , active presynaptic varicosities contiguous to dendritic clusters of the glutamate receptor R 1 . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The glutamate synthetase gene ( GS ) contains a composite glucocorticoid response element ( cGRE ) comprised of a GRE and an adjacent element with features of both a cAMP response element ( CRE ) and a 12 O tetradecanoylphorbol 13 acetate ( TPA ) response element ( TRE ) . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| HPLC analysis of the perfusate obtained by microdialysis in vivo showed a significant increase in the extracellular levels of aspartate , glutamate , glycine and taurine , and a decrease of the glutamine level during TPA infusion . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| This effect was mimicked by co treatment with a growth factor ( aFGF , bFGF or BDNF ; but not GDNF , IGF 1 , EGF or TGF ) and the neurotransmitter 5 HT ( but not GABA , dopamine , glutamate ) and / or a protein kinase activator ( IBMX , forskolin , TPA ) . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Whereas glutamate receptor mediated events are important in cerebral ischemia and tPA has previously been shown to enhance excitotoxicity in hippocampus , we hypothesized that tPA would exaggerate oxygen glucose deprivation ( OGD ) injury in cultures of hippocampal neurons . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Blockade of N methyl D aspartate ( NMDA ) glutamate receptors with the non competitive antagonist MK 801 or the competitive antagonist AP 5 attenuated TPA induced CREB , Elk 1 , and ERK1 / 2 phosphorylation . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of ERK1 / 2 induced by TPA and co application of high potassium and glutamate was greatly attenuated by preincubating Purkinje cells with the MEK1 / 2 ( MAPK ERK kinase 1 / 2 ) inhibitor PD 98059 . ^^^ The MEK1 / 2 inhibitor also suppressed declustering of the ionotropic glutamate receptor subunit 2 / 3 ( GluR2 / 3 ) induced by TPA and co application of high potassium and glutamate , even though phosphorylation of Ser 880 of GluR2 / 3 was not inhibited significantly in the presence of PD 98059 . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Pretreatment of the cerebellar cells with L glutamate and 12 O tetradecanoyl phorbol 13 acetate ( TPA ) , a known Ca2+ / diacylglicerol dependent protein kinase ( PKC ) activator , produced a decrease in [ 3H ] D aspartate uptake . ^^^ In sharp contrast , in cortical astrocytes , both L glutamate and TPA pre exposure result in an augmentation of the [ 3H ] D aspartate uptake . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Tissue plasminogen activator ( tPA ) , a serine protease immediate early gene product expressed in brain areas important in learning and memory , has been shown to cleave the NR 1 subunit of the NMDA receptor leading to a potentiated Ca ( 2+ ) influx . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Incubation with 12 O tetradecanoylphorbol 13 acetate ( TPA ) , a protein kinase C activator , caused a significant increase in glutamate uptake and completely reversed MPP+ induced inhibitory effect on glutamate uptake . ^^^ MPP ( + ) induced inhibition of glutamate uptake was due to the dysfunction of glutamate transporters ; TPA enhanced glutamate uptake and completely reversed the inhibitory effect of MPP+ . . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Besides clot lysis per se , tPA may have pleiotropic actions in the brain , including direct vasoactivity , cleaveage of the N methyl D aspartate ( NMDA ) NR 1 subunit , amplification of intracellular Ca++ conductance , and activation of other extracellular proteases from the matrix metalloproteinase ( MMP ) family , e . g . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| By interacting with the NMDA type glutamate receptor , tPA may amplify potentially excitotoxic calcium currents . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Here , we show tPA activity is rapidly increased in hippocampal neurons after glutamate stimulation . ^^^ Furthermore , the mRNA encoding tPA is present in dendrites and is rapidly polyadenylated after glutamate stimulation . ^^^ Both the polyadenylation of tPA mRNA and the subsequent increase in tPA protein is dependent on metabotropic glutamate receptor ( mGluR ) activation . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Moreover , i . p . treatment with alpha , beta amyrin was able to reduce the nociception produced by 8 bromo cAMP ( 8 Br cAMP ) and by 12 O tetradecanoylphorbol 13 acetate ( TPA ) or the hyperalgesia caused by glutamate . ^^^ |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In this study , we injected kainic acid ( KA ) , a glutamate receptor agonist that specifically hyperstimulates non NMDA type receptors , into the vitreous humor of CD 1 mice and have investigated the role of plasminogen activators ( PAs ) [ tissue plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) ] in excitotoxicity induced retinal damage . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Memantine , which is known to be a moderate affinity , uncompetitive , NMDA receptor antagonist , was investigated with regard to its ability to block the glutamate overstimulation and tissue plasminogen activator ( tPA ) / urokinase plasminogen activator ( uPA ) / matrix metalloproteinase ( MMP ) 9 modulation in experimental ICH . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| We examine here the potentially interrelated roles of tPA , NO and peroxynitrite ( ONOO ) , which is the toxic metabolite of NO , in BBB breakdown and neurodegeneration following intrahippocampal injection of the glutamate analog kainite ( KA ) . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Here we show that glutamate treatment leads to an increase in ROS production and activation of AP 1 , but not NFkappaB . 12 O Tetradecanoylphorbol 13 acetate ( TPA ) , an activator of protein kinase C and an inducer of NFkappaB and AP 1 , protected the cells . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| We tested the effects of tissue plasminogen activator ( t PA ) with the glutamate antagonist MK 801 or the calcium channel blocker nimodipine in an embolic stroke model . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The effect of glutamine replacement by glutamate and the balance between glutamate and glucose metabolism on the redistribution of t PA producing recombinant CHO cells metabolism is studied in a series of glucose shift down and shift up experiments in continuous culture . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| When depolarized , cortical neurons release bio active t PA that interacts with and cleaves the NR 1 subunit of the NMDA receptor . ^^^ |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00750 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|