| Interacting proteins: Q9ULH1 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Using affinity chromatography and yeast two hybrid interaction screens , we identified ASAP 1 as a major binding partner of protein tyrosine kinase focal adhesion kinase ( FAK ) . ^^^ Glutathione S transferase pull down and coimmunoprecipitation assays showed the binding of ASAP 1 to FAK is mediated by an interaction between the C terminal SH 3 domain of ASAP 1 with the second proline rich motif in the C terminal region of FAK . ^^^ In contrast , overexpression of a truncated variant of ASAP 1 that failed to bind FAK or a catalytically inactive variant of ASAP 1 lacking GAP activity resulted in a less pronounced inhibition of cell spreading . ^^^ Transient overexpression of wild type ASAP 1 prevented the efficient organization of paxillin and FAK in focal adhesions during cell spreading , while failing to significantly alter vinculin localization and organization . ^^^ We conclude from these studies that modulation of ARF activity by ASAP 1 is important for the regulation of focal adhesion assembly and / or organization by influencing the mechanisms responsible for the recruitment and organization of selected focal adhesion proteins such as paxillin and FAK . . ^^^ |
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| Interacting proteins: Q9ULH1 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Astler Coller B 1 and B 2 colorectal cancers differed significantly in DNA copy number of the genes , MOS ( p=0 . 04 ) , MYC ( p=0 . 007 ) , DDEF 1 ( p=0 . 004 ) , PTK 2 ( p=0 . 02 ) and PTP4A3 ( p=0 . 04 ) . ^^^ When comparing these with Astler Coller D primary tumors , significant differences were seen for several genes as well ( MYC ( p < 0 . 000 ) , DDEF 1 ( p < 0 . 000 ) , SLA ( p < 0 . 000 ) , PTK 2 ( p < 0 . 000 ) , PTP4A3 ( p=0 . 002 ) , and RECQL 4 ( p=0 . 01 ) ) . ^^^ |
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| Interacting proteins: Q9ULH1 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9ULH1 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|