| METHODS : Interaction of FAK with the C terminus of the hSlo alpha subunit of BK was investigated using the yeast two hybrid system as well as immunofluorescence microscopy and coimmunoprecipitation experiments with a rabbit anti hslo antibody on MG 63 and CAL 72 human osteosarcoma cell lines and on normal human osteoblasts . ^^^ Mapping of the FAK region interacting with hSlo was approached by testing the ability of hSlo to recruit mutated ot truncated FAK proteins . ^^^ RESULTS : To the best of our knowledge , we provide the first evidence of the physical association of FAK with the intracellular part of hslo . ^^^ We show that FAK / hSlo interaction likely takes place through the Pro 1 rich domain situated in the C terminal region of the kinase . ^^^ FAK / hSlo association occurs constitutively at a low , but appreciable , level in human osteosarcoma cells and normal human osteoblasts that express endogenous FAK and hSlo . ^^^ |