| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.62680178 |
| Cas interacts with focal adhesion plaques and is phosphorylated by the tyrosine kinases FAK and Src . 0.62680178^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.56174283 |
| FAK associates with several different signaling proteins such as Src family PTKs , p130Cas , Shc , Grb 2 , PI 3 kinase , and paxillin . 0.56174283^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.53012652 |
| While the FAK directly binds to Cas SH 3 , our findings imply that SH 3 binding molecule ( s ) other than FAK might regulate Cas phosphorylation , at least in FAK / cells . 0.53012652^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.84844908 |
| Considering that Cas associates with FAK , paxillin , and other molecules involved in the integrin signaling pathway , these results suggest that caspase mediated cleavage of Cas contributes to the disassembly of focal adhesion complexes and interrupts survival signals from the extracellular matrix . . 0.84844908^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.70850403 |
| Subsequent studies revealed that pp125FAK binds Cas L on its SH 3 domain and phosphorylates its tyrosine residues upon beta 1 integrin stimulation . 0.70850403^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.50985532 |
| Expression of the Cas binding proline rich region 1 of FAK hindered association of Cas with FAK and impaired the structural stability of sarcomeres . 0.50985532^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Crk associated substrate ( CAS ) family members appear to play a pivotal role in FAK regulation of cell migration . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Among these are the focal adhesion proteins p130cas ( Cas ) and focal adhesion kinase ( FAK ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These protein substrates have included the focal adhesion kinase ( FAK ) , cortactin , AFAP 110 , p120CAS , and p130CAS . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of p130cas but not of paxillin is stimulated after FAK expression . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These drugs also reduced the tyrosine phosphorylation of FAK and an associated factor , p130Cas . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| METHODS : Indirect immunofluorescence was used to label beta ( 1 ) integrin , FAK , paxillin , and p130CAS . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| They include the activation of the family of Rho GTPases , p130cas , and focal adhesion kinase ( FAK ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The docking protein p130Cas has , together with FAK , been found as a target of the Yersinia virulence effector YopH . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Integrin mediated cell adhesion involves p 130 ( CAS ) association with focal adhesion kinase ( p 125 ( FAK ) ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We observe increased CrkII complex formation with p 130 ( Cas ) , focal adhesion kinase ( FAK ) , and Shb in PC 12 GTK cells . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| PAF exposure induced binding of p 130 ( Cas ) , Src , SHC , and paxillin to FAK . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Complexes of focal adhesion kinase ( FAK ) and Crk associated substrate ( p 130 ( Cas ) ) are elevated in cytoskeleton associated fractions following adhesion and Src transformation . ^^^ The focal adhesion kinase ( FAK ) and Crk associated substrate , p 130 ( Cas ) ( Cas ) , have been implicated in diverse signaling pathways including those mediated by integrins , G protein coupled receptors , tyrosine kinase receptors , and the 5 src and 5 crk oncogenes . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Integrin ligand binding induces the tyrosine phosphorylation of various proteins including focal adhesion kinase ( pp 125 ( FAK ) ) and Crk associated substrate ( Cas ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Cleavage of pp125FAK differentially affects its association with signaling and cytoskeletal components of the focal adhesion complex ; binding of paxillin , but not pp 130 ( Cas ) ( Cas , Crk associated substrate ) and vinculin , to the COOH terminally truncated pp125FAK is abolished . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Several of these proteins were identified and include protein tyrosine kinase 2 ( also known as CAKbeta , RAFTK , and CADTK ) , pp 125 focal adhesion tyrosine kinase , pp 130 Crk associated substrate , paxillin , and Cbl . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Here , the effects of PTEN on cell invasion , migration , and growth as well as the involvement of FAK and p 130 Crk associated substrate ( p130Cas ) were investigated in U87MG glioblastoma cells missing PTEN . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The phosphorylated proteins include focal adhesion kinase ( FAK ) , paxillin and Crk associated substrate , p130Cas , all of which are known to be associated with focal adhesions . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We previously described inhibition of cell migration by PTEN and restoration of motility by focal adhesion kinase ( FAK ) and p 130 Crk associated substrate ( p 130 ( Cas ) ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In parallel , IGF IR activation induced rapid and transient tyrosine dephosphorylation of focal adhesion proteins p 125 focal adhesion kinase ( FAK ) , p 130 Crk associated substrate ( Cas ) , and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In cardiomyocytes cultured on ECM , PE stimulated a rapid increase in tyrosine phosphorylation of focal adhesion proteins including FAK , paxillin , and p 130 Crk associated substrate and subsequent formation of peripheral focal complexes . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Abbreviations : CAS , CRK associated substrate ; CH , calponin homology domain ; CSK , C terminal SRC kinase ; E 6 , Papillomavirus E 6 protein ; FAK , focal adhesion kinase ; GIT , GRK interacter ; GPCR , heterotrimeric G protein coupled receptor ; GRK , G protein coupled receptor kinase ; MAPK , mitogen activated protein kinase ( ERK , p 38 , JNK ) ; PAK , p 21 activated kinase ; PBS , paxillin binding subdomain ; PIX , PAK interacting exchange factor ; PKL , paxillin kinase linker ; POR 1 , partner of Rac ; PS , phosphoserine ; PT , phosphothreonine ; PY , phosphotyrosine ; RTK , growth factor receptor tyrosine kinase ; SH , SRC homology domain . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We now show that the cytoplasmic tyrosine kinase , focal adhesion kinase ( FAK ) plays an essential role in the beta 1 integrin stimulated migration of T cells through regulation of the unique Crk associated substrate ( Cas ) family docking protein , human enhancer of filamentation 1 ( HEF 1 ) and effects on `` outside in ' ' beta 1 integrin signaling . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Our results portray FAK as a major 5 Src substrate that also plays a role in recruiting 5 Src to phosphorylate substrates CAS ( Crk associated substrate ) and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Crk associated substrate ( Cas ) , Nck , and focal adhesion kinase ( FAK ) were also phosphorylated after SDF 1alpha stimulation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Among the host cell targets of YopH are the focal adhesion proteins Crk associated substrate ( p130Cas ) and focal adhesion kinase ( FAK ) in epithelial cells , and p130Cas and Fyn binding protein ( Fyb ) in macrophages . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Src family kinases recruited to the Tyr 397 site phosphorylate two FAK interacting proteins , Crk associated substrate ( CAS ) and paxillin , which results ultimately in regulation of Rho family GTPases contributing to cell motility . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| As a downstream target of FAK Lyn signaling , the p130CAS ( Crk associated substrate ) protein was decreased upon the expression of KAI1 / CD82 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Src activation failed to increase the high basal tyrosine phosphorylation of the Crk associated substrate , CAS , found in FAK / MEF , indicating that CAS phosphorylation alone is insufficient to induce motility in the absence of FAK or 5 Src induced cytoskeletal remodeling . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The purpose of the present study was to determine the effects of G 17 amide on tyrosine phosphorylation of focal adhesion kinase ( FAK ) , paxillin , and p 130 Crk associated substrate ( p 130 ( Cas ) ) in Colo 320 cells , a human colorectal cancer cell line which expresses CCK ( 2 ) receptors . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| CrkII overexpression enhanced the autophosphorylation of FAK at Tyr 397 and tyrosine phosphorylation of p 130 ( Cas ) ( Crk associated substrate , Cas ) upon stimulation of integrin by fibronectin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Complex formation of FAK with JSAP 1 and p 130 Crk associated substrate ( p 130 ( Cas ) ) resulted in augmentation of FAK activity and phosphorylation of both JSAP 1 and p 130 ( Cas ) , which required p 130 ( Cas ) hyperphosphorylation and was abolished by inhibition of Src . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We identified p 125 focal adhesion kinase ( FAK ) , p 130 Crk associated substrate ( CAS ) and paxillin as prominent targets of gastrointestinal peptide stimulated tyrosine phosphorylation and developed a model that envisages a G12 / Rho dependent pathway connecting GPCR activation to the tyrosine phosphorylation of these focal adhesion proteins . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Neuronal precursor cell expressed , developmentally down regulated gene ( Nedd ) 9 was recognized to be identical to Crk associated substrate lymphocyte type ( Cas L ) , a docking protein that associates with a variety of signaling molecules , such as focal adhesion kinase ( FAK ) , proline rich tyrosine kinase 2 ( Pyk 2 ) , and Crk . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| FAK overexpression and PDGF stimulation was found to increase the phosphorylation of the Crk associated substrate ( CAS ) family member human enhancer of filamentation 1 ( HEF 1 ) , but not p130CAS or Src interacting protein ( Sin ) / Efs , although the levels of expression of these proteins was similar . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Here we studied molecular interactions and tyrosine phosphorylation of paxillin , Crk associated substrate ( CAS ) , and focal adhesion kinase ( FAK ) in focal adhesions . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The screen identified two proteins that interact with FAK via their Src homology 3 ( SH 3 ) domains : a 5 Crk associated tyrosine kinase substrate ( Cas ) , p130Cas , and a still uncharacterized protein , FIPSH 3 2 , which contains an SH 3 domain closely related to that of p130Cas . ^^^ Coimmunoprecipitation experiments confirmed that p130Cas and FAK are associated in mouse fibroblasts . ^^^ The stable interaction between p130Cas and FAK emerges as a likely key element in integrin mediated signal transduction and further represents a direct molecular link between the 5 Src and 5 Crk oncoproteins . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Activation of PKC by adding phorbol ester to alpha 5 beta 5 expressing cells induced spreading , increased colocalization of alpha actinin , tensin , vinculin , p130cas and actin , and triggered tyrosine phosphorylation of FAK . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Deduced amino acid sequence revealed that pp 105 contains conserved motifs with p130Cas , and both pp 105 and p130Cas bind to focal adhesion kinase ( pp125FAK ) and Crk . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Tyr ) and Grb 2 binding to FAK were reduced , whereas the tyrosine phosphorylation of another signaling protein , p130cas , was not detected in the Src cells . ^^^ Stable expression of residues 1 to 298 of Src ( Src 1 298 , which encompass the SH 3 and SH 2 domains of c Src ) in the Src cells blocked Grb 2 binding to FAK ; but surprisingly , Src 1 298 expression also resulted in elevated p130cas P . ^^^ Src 1 298 bound to both FAK and p130cas and promoted FAK association with p130cas in vivo . ^^^ FAK was observed to phosphorylate p130cas in vitro and could thus phosphorylate p130cas upon FN stimulation of the Src 1 298 expressing cells . ^^^ FAK induced phosphorylation of p130cas in the Src 1 298 cells promoted the SH 2 domain dependent binding of the Nck adaptor protein to p130cas , which may facilitate signaling to ERK 2 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The PTPase YopH inhibits uptake of Yersinia , tyrosine phosphorylation of p130Cas and FAK , and the associated accumulation of these proteins in peripheral focal adhesions . ^^^ We show that p130Cas and FAK are phosphorylated and recruited to peripheral focal complexes during bacterial uptake in HeLa cells . ^^^ The inactive form of YopH interacts with the tyrosine phosphorylated forms of FAK and p130Cas and co localizes with these proteins in focal adhesions . ^^^ On the other hand , the presence of active YopH results in inhibition of uptake , dephosphorylation of p130Cas and FAK , and disruption of peripheral focal complexes . ^^^ We suggest that p130Cas and FAK are substrates for YopH and that the dephosphorylation of these proteins impairs the uptake of Yersinia pseudotuberculosis into HeLa cells . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Both pp 105 and pp 115 are antigenically distinct from HEF 1 , p130Cas , pp125FAK , Pyk 2 , p120cbl , and the p 110 subunit of phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| FAK / Src association activates both kinases , which act on the potential substrates tensin , paxillin and p130cas . ^^^ Besides cytoskeletal regulation , FAK phosphorylation of paxillin and p130cas could also lead to MAP kinase pathway by adaptor proteins such as Crk and Nck . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Although it remains to be determined which protein tyrosine kinase ( s ) is involved in this response , pp 130 tyrosine phosphorylation appears to be a specific and early signal transmitted after the interaction of FRP 1 with a specific antibody . pp 130 was present in the cytosol fraction and was distinct from pp125FAK , p130CAS , vinculin , and beta 1 integrin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The activated FAK / Src complex acts on potential substrates tensin , paxillin and p130cas . ^^^ Besides cytoskeletal regulation , FAK phosphorylation and / or binding to paxillin and p130cas may trigger downstream activation of MAP kinase by the adoptor protein Crk . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These effects on transformation correlate with the phosphorylation status of p130Cas and two proteins that are associated with p130Cas , Paxillin and Fak . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic focal adhesion proteins vinculin , talin , paxillin , p130CAS , and pp125FAK were detected , although vinculin appeared to be confined mainly to the mesangium . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In the H 9 T cell line , beta 1 integrin engagement leads to the increased tyrosine phosphorylation of three 105 to 115 kDa substrates that are distinct from focal adhesion kinase ( FAK ) : HEF 1 ( human enhancer of filamentation 1 ) , a protein with structural homology to p130Cas , and two novel substrates , pp 105 and pp 115 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We found that a focal adhesion kinase ( FAK ) related protein , cell adhesion kinase beta ( CAKbeta ) , was bound in vitro by the SH 3 domain of embryonal Fyn associated substrate ( Efs ) , a docking protein structurally related to p130Cas ( Cas ) and HEF 1 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| FAK interacts with a number of signaling and cytoskeletal proteins , including Src , phosphatidylinositol 3 kinase , Grb 2 , p130Cas and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Platelet intracellular Ca2+ mobilization induced by thrombin was hardly inhibited by these PKC inhibitors . p130Cas is a docking protein that associates with FAK or PYK 2 through the SH 3 domain . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion kinase ( FAK ) plays a prominent role in the adhesion signaling pathway through its tyrosine kinase activity and protein protein interaction with other signaling molecules , including src , paxillin , and p130CAS , and other proteins . ^^^ FAK , paxillin , and p130CAS appeared to be tyrosine phosphorylated in both NIH / 3T3 and B 104 1 1 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| CVI induced tyrosine phosphorylation of proteins that associate with focal adhesions , such as paxillin , focal adhesion kinase ( FAK ) , and p130CAS . ^^^ Our results indicate that soluble fragments of native collagen 6 , a ubiquitous component of the interstitial extracellular matrix , can mediate stimulation of DNA synthesis via tyrosine phosphorylation of paxillin , FAK , p130CAS , and erk 2 in the absence of classical growth factors . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies confirm that the ability of pp 125 ( FAK ) to associate with paxillin , vinculin , and p130cas is significantly reduced in SMC treated with degraded collagen fragments . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These morphological changes appeared to be closely associated with degradation of focal adhesion proteins , including p130cas , p 125 ( FAK ) and paxillin . p130cas was also degraded in cells treated with staurosporine or etoposide , suggesting that degradation of focal adhesion proteins is a characteristic feature of apoptosis . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We report that binding of the SH 3 domain of p130Cas to proline rich region 1 of FAK is required to support survival of fibroblasts on fibronectin when serum is withdrawn . ^^^ The FAK p130Cas complex activates c Jun NH 2 terminal kinase ( JNK ) via a Ras / Rac1 / Pak1 / MAPK kinase 4 ( MKK 4 ) pathway . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of cell lysates adherent to placental laminin showed that the tyrosine phosphorylation of p130Cas and FAK was maximally above constitutive levels after 60 min . ^^^ In cells adherent to EHS laminin ( laminin 1 ) , the tyrosine phosphorylation kinetics of tensin , p130Cas , FAK and unknown proteins of 138 kDa and 110 kDa were similar , and peaked above constitutive levels after 30 min . ^^^ However , phosphorylation and activation kinetics of Erk 2 in cells adherent to placental laminin was similar to that observed for FAK and p130Cas . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemistry , sucrose density gradient sedimentation , co immunoprecipitation analyses and in vitro binding assays have shown that polycystin 1 associates with the focal adhesion proteins talin , vinculin , p130Cas , FAK , alpha actinin , paxillin and pp60c src in subconfluent normal human fetal collecting tubule ( HFCT ) epithelia when cell matrix interactions predominate . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In contrast , all other tyrosine phosphorylated forms of Fak and Pyk 2 are predominantly localized to focal adhesions and the cell periphery in motile cells , all colocalized with paxillin and p130Cas . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| To identify the tyrosine phosphorylated proteins required for osmotic shock stimulated glucose uptake , we examined tyrosine phosphorylation of candidate proteins , and found that the 60 80kDa species including paxillin and the 120 130kDa species including p130Cas , PYK 2 , FAK and Gab 1 were tyrosine phosphorylated in response to osmotic shock . ^^^ Inhibition of actin polymerization by cytochalasin D significantly decreased the tyrosine phosphorylation of paxillin , p130Cas , PYK 2 and FAK but not Gab 1 , but had no effect on 2 deoxyglucose ( DOG ) uptake , suggesting a role for Gab 1 in osmotic shock induced glucose transport . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of paxillin , FAK , and p130CAS : effects on cell spreading and migration . ^^^ This review focuses on three of the cytoskeletal components of the focal adhesion , paxillin , FAK , and p130CAS , that are phosphorylated and play a regulatory role in cell spreading and cell migration . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Paxillin associates with numerous signaling molecules including adaptor molecules ( p130Cas , CRK ) , kinases ( FAK , Pyk 2 , PAK and SRC ) , tyrosine phosphatases ( PTP PEST ) , ARF GAP proteins ( p95pkl , PAG 3 ) and papillomavirus E 6 oncoproteins . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition , Ang 2 activates many intracellular tyrosine kinases that play a role in growth signaling and inflammation , such as Src , Pyk 2 , p130Cas , FAK and JAK / STAT . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| FRNK expression disrupted the formation of a 5 Src FAK signaling complex , inhibited p130Cas tyrosine phosphorylation , and attenuated 5 Src stimulated ERK and JNK kinase activation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Activities of focal adhesion kinase ( FAK ) and its downstream p130Cas were examined by Western blotting . ^^^ RPMC formed focal adhesions on FN in the presence of a regular glucose concentration ( 5 . 6 mmol / L ) ; however , tyrosine phosphorylation of FAK and p130Cas and formation of focal adhesions observed by FAK and vinculin staining were substantially inhibited by high glucose . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We found that focal adhesion complex proteins , including focal adhesion kinase ( FAK ) , talin , paxillin , and p130cas , but not vinculin , were decreased within 1 h when MDCK cells were cultured on collagen gel . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Among the PKC downstream signal molecules , p130Cas , a mediator of cell migration , and its kinase , focal adhesion kinase ( FAK ) , increased following TPA treatment ; phosphorylation of p130Cas was induced in a PKC alpha dependent manner . ^^^ Together , these results demonstrate that PKC alpha promotes GT 1 neuronal migration by activating focal adhesion complex proteins such as p130Cas and FAK . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| RGD dose response experiments revealed that c Src activation occurs subsequent to its cytoskeletal recruitment and is accompanied by p130Cas cytoskeletal binding and focal adhesion kinase ( FAK ) Tyr 925 phosphorylation . ^^^ Together these data indicate that RGD treatment in cardiomyocytes causes beta 3 integrin activation and c Src sarcolemmal localization , that subsequent c Src activation is accompanied by p130Cas binding and FAK Tyr 925 phosphorylation , and that these events might be crucial for growth and remodeling of hypertrophying adult cardiomyocytes . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| VEGF induced the coupling of focal adhesion kinase ( FAK ) to integrin alphavbeta 5 and tyrosine phosphorylation of the cytoskeletal components paxillin and p130cas . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Ionizing radiation modules of the expression and tyrosine phosphorylation of the focal adhesion associated proteins focal adhesion kinase ( FAK ) and its substrates p130cas and paxillin in A 549 human lung carcinoma cells in vitro . ^^^ Therefore , study was performed to determine the effect of IR on the expression and phosphorylation of FAK and two of its substrates , p130cas and paxillin , in vitro . ^^^ Three of these proteins were identified as FAK , p130cas and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Using these assays , we show that kinases and adaptor molecules , including focal adhesion kinase ( FAK ) , Src , p130CAS , paxillin , extracellular signal regulated kinase ( ERK ) and myosin light chain kinase ( MLCK ) are critical for adhesion turnover at the cell front , a process central to migration . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we found that the cleavage of p130Cas , as well as another focal adhesion component FAK , is different from that of caspase substrate PARP and spectrin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that MDA MB 231 cells with encumbered motility due to forced re expression of FGF 2 have activated focal complexes as determined by immunoprecipitation / western blotting and immunofluorescence staining with antibodies to FAK , p130Cas , paxillin , vinculin and phosphotyrosine . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Lamellipodia production , or function , is defective and there is a selective reduction in the level and tyrosine phosphorylation of FAK , p130Cas , Crk , and Dock 180 at nascent focal complexes . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Although p130cas siRNA inhibited cell spreading on collagen 4 by 33 % , three different paxillin siRNAs did not inhibit cell spreading . p130cas siRNA did not affect Src Tyr 416 or Src Tyr 527 phosphorylation , FAK Tyr 397 phosphorylation , or Src dependent phosphorylation of FAK Tyr 925 , suggesting that p130cas did not inhibit cell spreading by altering FAK or Src activity . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition to colony formation , protein kinase B / Akt ( PKB / Akt ) kinase activity , focal adhesion kinase ( FAK ) , p130Cas , paxillin and c Jun N 2 terminal kinase ( JNK ) expression and phosphorylation were analyzed by Western blot technique . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Kit modulated phosphorylation dependent interactions with pathways controlling focal adhesion ( paxillin , leupaxin , p130CAS , FAK 1 , the Src family kinase Lyn , Wasp , Fhl 3 , G25K , Ack 1 , Nap 1 , SH3P12 / ponsin ) and septin actin complexes ( NEDD 5 , cdc 11 , actin ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In the present study , we investigated the effect of neonatal cerebral hypoxia ischemia ( HI ) on levels and tyrosine phosphorylation of focal adhesion kinase and the interaction of this enzyme with Src protein tyrosine kinase and adapter protein p130Cas , involved in FAK mediated signaling pathway . ^^^ Concomitantly a decreased association of FAK with its investigated molecular partners , Src kinase and p130Cas protein has been observed . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Ang 2 induced AT1R activation via Gq / 11 stimulates phospholipases A 2 , C , and D , and activates inositol trisphosphate / Ca2+ signaling , protein kinase C isoforms , and MAPKs , as well as several tyrosine kinases ( Pyk 2 , Src , Tyk 2 , FAK ) , scaffold proteins ( G protein coupled receptor kinase interacting protein 1 , p130Cas , paxillin , vinculin ) , receptor tyrosine kinases , and the nuclear factor kappaB pathway . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| As p130Cas phosphorylation and Rac activation are common downstream targets for alpha5beta1 stimulated FAK activation , our results support the existence of a novel alpha 4 cytoplasmic domain connection leading to c Src activation which functions as a FAK independent linkage to a common motility promoting signaling pathway . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| CD 82 expression also reduced integrin induced activation and phosphorylation of the cytoplasmic tyrosine kinase Src , and its downstream substrates p130Cas and FAK Y 861 . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Last , decreased tyrosine phosphorylation of FAK substrates p130Cas and paxillin were observed in CFKO mice compared with the control littermates . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The kinetic profile of Cas phosphorylation was almost identical with that of tyrosine phosphorylation of focal adhesion kinase pp125FAK ( Fak ) , which is well known to be activated subsequent to integrin mediated cell adhesion . ^^^ These results suggest that tyrosine phosphorylation of Cas is stimulated by normal cell adhesion in close association with Fak phosphorylation and the formation of actin stress fibers . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Sequences necessary for interacting with the focal adhesion kinase pp125FAK ( FAK ) , 5 SRC and 5 CRK have been mapped to distinct regions of CAS . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that p 130 ( Cas ) associates both in vitro and in vivo with pp 125 ( FAK ) ( focal adhesion kinase ) , a kinase implicated in signaling by the integrin family of cell adhesion receptors . p 130 ( Cas ) also associates with pp41 / 43 ( FRNK ) ( pp 125 ( FAK ) related , non kinase ) , an autonomously expressed form of pp 125 ( FAK ) composed of only the C terminal noncatalytic domain . ^^^ We show that the association of p 130 ( Cas ) with pp 125 ( Fak ) and pp41 / 43 ( FRNK ) is direct , and is mediated by the binding of the SH 3 domain of p 130 ( Cas ) to a proline rich sequence present in both the C terminus of pp 125 ( FAK ) and in pp41 / 43 ( FRNK ) . ^^^ The association of p 130 ( Cas ) with pp 125 ( FAK ) , a kinase which is activated upon cell adhesion , is likely to be functionally important in integrin mediated signal transduction . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In normal cells , the focal adhesion proteins tensin , p 125 ( FAK ) , and paxillin constitutively associated with p 130 ( CAS ) . ^^^ However , in BCR / ABL transformed cells , the interaction between p 130 ( CAS ) and tensin was disrupted , while the associations between p 130 ( CAS ) , p 125 ( FAK ) , and paxillin were unaffected . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| A signaling partnership is formed between FAK and Src family kinases , leading to tyrosine phosphorylation of FAK and associated ' docking ' proteins Cas and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Expression of wild type and Phe 925 FAK , but not Phe 397 FAK , enhanced p 130 ( Cas ) association with FAK , Shc tyrosine phosphorylation , and Grb 2 binding to Shc after FN stimulation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In adherent GN 4 cells , the constitutive activity of p 125 ( FAK ) was correlated with basal paxillin , tensin , and p 130 ( CAS ) tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Vanadate treatment of pp 125 ( FAK ) and CAKbeta overexpressing CE cells induced a dramatic increase in the phosphotyrosine content of a common set of proteins including tensin , paxillin , and p 130 ( Cas ) , but some of these substrates , particularly p 130 ( Cas ) , appeared to be differentially phosphorylated by pp 125 ( FAK ) and CAKbeta . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In contrast , insulin had no effect on CADTK but stimulated the tyrosine phosphorylation of Cbl and the tyrosine dephosphorylation of pp 125 ( FAK ) and p 130 ( cas ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In this report , we have examined the role of FAK association with Grb 2 and p 130 ( Cas ) , two downstream events of the FAK / Src complex that could mediate integrin stimulated activation of extracellular signal regulated kinases ( Erks ) . ^^^ This mutation did not affect FAK kinase activity , autophosphorylation , or Src association but did significantly reduce p 130 ( Cas ) association with FAK . ^^^ Furthermore , FAK expression in CHO cells increased tyrosine phosphorylation of p 130 ( Cas ) and its subsequent binding to several SH 2 domains , which depended on both the p 130 ( Cas ) binding site and the Src binding site . ^^^ Together , these results demonstrate that p 130 ( Cas ) , but not Grb 2 , is a mediator of FAK promoted cell migration and suggest that FAK / p 130 ( Cas ) complex targets downstream pathways other than Erks in mediating FAK promoted cell migration . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Furthermore , a Cas L mutant that lacks the SH 3 domain , the binding site for focal adhesion kinase ( FAK ) , is also tyrosine phosphorylated upon CD 3 cross linking , but not upon beta 1 integrin crosslinking , suggesting that FAK is not involved in CD 3 dependent Cas L phosphorylation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The focal adhesion kinase ( FAK ) functions in regulating tyrosine phosphorylation of several of these proteins , including paxillin , tensin , and p 130 ( cas ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Analysis by immunoblotting demonstrated tyrosine phosphorylation of focal adhesion kinase ( FAK ) , the focal adhesion associated proteins paxillin and p 130 ( cas ) , and mitogen activated protein kinase ( MAPK ) following the occupancy of the uPAR by uPA . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| IGF 1 also promoted the formation of a complex between p 130 ( Cas ) and c Crk and elicited a parallel increase in the tyrosine phosphorylation of p 125 ( Fak ) and paxillin . ^^^ IGF 1 induced p 130 ( Cas ) , p 125 ( Fak ) , and paxillin tyrosine phosphorylation could be dissociated from mitogen activated protein kinase kinase , p 70 ( S6K ) , and protein kinase C activation . ^^^ In contrast , the structurally unrelated phosphatidylinositol 3 kinase inhibitors wortmannin and LY 294002 markedly attenuated the increase in tyrosine phosphorylation of p 130 ( Cas ) , p 125 ( Fak ) , and paxillin induced by IGF 1 . ^^^ Cytochalasin D , which disrupts the network of actin microfilaments , completely prevented tyrosine phosphorylation of p 130 ( Cas ) , p 125 ( Fak ) , and paxillin and the formation of a p 130 ( Cas ) . ^^^ Thus , our results identified a phosphatidylinositol 3 kinase dependent pathway that requires the integrity of the actin cytoskeleton to induce tyrosine phosphorylation of p 130 ( Cas ) , p 125 ( Fak ) , and paxillin in response to IGF 1 and suggest that tyrosine phosphorylation of these focal adhesion proteins , together with the recruitment of c Crk into a complex with p 130 ( Cas ) , may play a novel role in IGF 1 signal transduction . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We now show here in Chinese hamster ovary cells stably transfected with rat GH receptor cDNA that human ( h ) GH induces the formation of a large multiprotein signaling complex centered around another FAK associated protein , p 130 ( Cas ) and the adaptor protein CrkII . hGH stimulates the tyrosine phosphorylation of both p 130 ( Cas ) and CrkII , their association , and the association of multiple other tyrosine phosphorylated proteins to the complex . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The expression of other Src substrates and interacting proteins , such as p 120 Cas , p 130 Cas , vinculin , Fak kinase , and the p 85 phosphatidylinositol 3 kinase subunit either did not change or slightly increased during PMA treatment . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Dissociation of FAK / p130 ( CAS ) / c Src complex during mitosis : role of mitosis specific serine phosphorylation of FAK . ^^^ We have found that components of focal adhesions including focal adhesion kinase ( FAK ) , paxillin , and p 130 ( CAS ) ( CAS ) are serine / threonine phosphorylated during mitosis when all three proteins are tyrosine dephosphorylated . ^^^ First , the association of FAK with CAS or c Src is greatly inhibited , with levels decreasing to 16 and 13 % of the interphase levels , respectively . ^^^ Mitosis specific phosphorylation is responsible for the disruption of FAK / CAS binding because dephosphorylation of mitotic FAK in vitro by protein serine / threonine phosphatase 1 restores the ability of FAK to associate with CAS , though not with c Src . ^^^ These results suggest that mitosis specific modification of FAK uncouples signal transduction pathways involving integrin , CAS , and c Src , and may maintain FAK in an inactive state until post mitotic spreading . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| This phenomenon appears to be due in part to a constitutive increase in tyrosine phosphorylation of p 130 ( CAS ) , a known PTP PEST substrate , paxillin , which associates with PTP PEST in vitro , and focal adhesion kinase ( FAK ) . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Immuno precipitation experiments revealed that paxillin , pp 125 ( FAK ) , and pp 130 ( CAS ) were included in the 70 kDa , and 120 130 kDa bands , respectively . ^^^ These results strongly suggest that cyclic stretch induces the activation of pp 60 ( src ) and that pp 60 ( src ) is indispensable for the tyrosine phosphorylation of pp 130 ( CAS ) , pp 125 ( FAK ) and paxillin followed by the orienting response in 3Y1 fibroblasts . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Together , these results strongly suggest that PI3K binding is required for FAK to promote cell migration and that the binding of Src and p 130 ( Cas ) to FAK may not be sufficient for this event . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In response to adhesion on a fibronectin substrate , RPTPalpha / fibroblasts also exhibited characteristic deficiencies in integrin mediated signalling responses , such as decreased tyrosine phosphorylation of the c Src substrates Fak and p 130 ( cas ) , and reduced activation of extracellular signal regulated ( Erk ) MAP kinases . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Negative effects on cell spreading and migration , as well as decreased phosphorylation of the substrate p 130 ( Cas ) , were observed upon induced expression of the FAK autophosphorylation site mutant . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Integrin mediated stimulation of JNK required the association of focal adhesion kinase ( FAK ) with a Src kinase and p 130 ( CAS ) , the phosphorylation of p 130 ( CAS ) , and subsequently , the recruitment of Crk . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Platelet activating factor stimulation of p 125 ( FAK ) and p 130 ( Cas ) tyrosine phosphorylation in brain . ^^^ These proteins were identified by immunoprecipitation as p 125 ( FAK ) and p 130 ( Cas ) , using monoclonal antibodies . ^^^ The finding that PAF stimulates tyrosine phosphorylation of both focal adhesion protein p 125 ( FAK ) and p 130 ( Cas ) suggests that PAF might modulate the integrin mediated signal transduction in the brain . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition , we found that phosphorylation of FAK or p 130 ( cas ) was not affected by the expression of either Grb 7 or its SH 2 domain alone , suggesting that Grb 7 is downstream of FAK and does not compete with Src for binding to FAK in vivo . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In this study , we have demonstrated that UV irradiation induced cleavage of FAK and two of its interacting proteins Src and p 130 ( Cas ) in Madin Darby canine kidney cells , concomitant with an increase in cell death . ^^^ Moreover , the expression of the Src homology 3 domain of p 130 ( Cas ) , which competed with endogenous p 130 ( Cas ) for FAK binding , abrogated the FAK promoted cell survival . ^^^ Together , these results suggest that the integrity of FAK and its binding to phosphatidylinositol 3 kinase and p 130 ( Cas ) are required for FAK to exert its antiapoptotic function . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We found no evidence for [ Ca ( 2+ ) ] ( c ) mediated signaling or for tyrosine phosphorylation of pp 125 ( FAK ) , p 130 ( CAS ) , and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| However , clustering of beta1B did not induce tyrosine phosphorylation of FAK , p 130 ( Cas ) , or paxillin , as studied by beta1B mediated adhesion , to fibronectin in the presence of Mn ( 2+ ) or to anti beta 1 antibody in DMEM . ^^^ Stimulation of tyrosine phosphorylation on FAK , p 130 ( Cas ) , and paxillin by adhesion via integrin alphaVbeta 3 to fibronectin or vitronectin was not disturbed in GD 25 beta1B cells compared to the untransfected GD 25 cells , nor were any negative effects of beta1B observed on alphaVbeta 3 mediated cell attachment , spreading , and actin organization , or on the cell proliferation rate . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that different agonists increase tyrosine phosphorylation of the focal adhesion related proteins p 125 ( FAK ) , p 130 ( Cas ) , and paxillin in different cell types and that tyrosine phosphorylation depends on the integrity of the actin cytoskeleton . ^^^ Addition of carbachol or CCK 8 to pancreatic acini resulted in rapid increases in the tyrosine phosphorylation of p 125 ( FAK ) , p 130 ( Cas ) , and paxillin . ^^^ Pretreatment of pancreatic acini with LY 294002 or wortmannin resulted in a concentration dependent inhibition of tyrosine phosphorylation of p 125 ( FAK ) , p 130 ( Cas ) , and paxillin stimulated by carbachol or CCK 8 . ^^^ These results indicate that m 3 muscarinic and CCK ( A ) receptor mediated increase in p 125 ( FAK ) , p 130 ( Cas ) , and paxillin tyrosine phosphorylation in pancreatic acini depends on the ability of these cells to synthesise phosphoinositides . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In our efforts to determine the mechanism by which FAK promotes HGF induced cell migration , we found that FAK mutants deficient in phosphatidylinositol 3 kinase or p 130 ( Cas ) binding failed to promote HGF induced cell migration . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of p 125 ( Fak ) , p 130 ( Cas ) , and paxillin does not require extracellular signal regulated kinase activation in Swiss 3T3 cells stimulated by bombesin or platelet derived growth factor . ^^^ The experiments presented here were designed to examine the contribution of the extracellular signal regulated mitogen activated protein kinases ( ERKs ) to the tyrosine phosphorylation of the focal adhesion proteins p 125 ( Fak ) , p 130 ( Cas ) , and paxillin induced by G protein coupled receptors ( GPCRs ) and tyrosine kinase receptors in Swiss 3T3 cells . ^^^ Exposure of the cells to two structurally unrelated mitogen activated protein kinase or ERK kinase ( MEK ) inhibitors , PD 98059 and U 0126 , completely abrogated ERK activation but did not prevent tyrosine phosphorylation of p 125 ( Fak ) , p 130 ( Cas ) , and paxillin . ^^^ Thus , our results demonstrate that the activation of the ERK pathway is not necessary for the increase of the tyrosine phosphorylation of p 125 ( Fak ) , p 130 ( Cas ) , and paxillin induced by either GPCRs or tyrosine kinase receptors in Swiss 3T3 cells . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Activation of m 3 muscarinic receptors induces rapid tyrosine phosphorylation of p 125 ( FAK ) , p 130 ( cas ) , and paxillin in rat pancreatic acini . ^^^ The present study was aimed at examining whether activation of m 3 muscarinic receptors in rat pancreatic acini evokes tyrosine phosphorylation of p 125 ( FAK ) , and its substrates , p 130 ( cas ) and paxillin . ^^^ Results show that stimulation of pancreatic acini with carbachol resulted in a rapid and transient increase in tyrosine phosphorylation of p 125 ( FAK ) , p 130 ( cas ) , and paxillin . ^^^ Simultaneous blockage of both PKC activation and increases in [ Ca ( 2+ ) ] ( 1 ) partially decreased p 125 ( FAK ) , p 130 ( cas ) , and paxillin tyrosine phosphorylation stimulated by carbachol . ^^^ Pretreatment of pancreatic acini with Clostridium botulinum C 3 transferase , which specifically inactivates p 21 ( rho ) , partially inhibited carbachol induced p 125 ( FAK ) , p 130 ( cas ) , and paxillin tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In contrast to Cas involvement in focal adhesions in other cells , platelet Cas phosphorylation preceded the activation of focal adhesion kinase ( FAK ) , and blockage of alpha IIb beta 3 mediated platelet aggregation with a GRGDS peptide resulted in prolongation of stimulation dependent Cas tyrosine phosphorylation but in suppression of FAK tyrosine phosphorylation . ^^^ The failure of FAK to associate with Cas in immunoprecipitation studies also suggests that Cas tyrosine phosphorylation is independent of FAK activation . ^^^ Finally , Cas existed mainly in cytosol and membrane cytoskeleton fractions in the resting state , and remained unchanged during platelet aggregation , when FAK translocated to the cytoskeletal fraction . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These include bombesin induced assembly of focal adhesions , formation of parallel arrays of actin stress fibers , increase in the tyrosine phosphorylation of focal adhesion kinase ( FAK ) , p 130 ( Cas ) , and paxillin , and formation of a complex between FAK and Src . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Contortrostatin , a snake venom disintegrin , induces alphavbeta 3 mediated tyrosine phosphorylation of CAS and FAK in tumor cells . ^^^ We found that at concentrations as low as 1 nM , soluble contortrostatin activates integrin signals leading to increased tyrosine phosphorylation of FAK and CAS , and that these signals are abolished by inhibiting Src family kinases . ^^^ We propose that the homodimeric nature of contortrostatin imparts the ability to crosslink alphavbeta 3 integrins , causing Src activation and hyperphosphorylation of FAK and CAS . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These changes are accompanied by cytoskeletal binding and phosphorylation of focal adhesion kinase ( FAK ) at Tyr 397 and Tyr 925 , c Src at Tyr 416 , recruitment of the adapter proteins p 130 ( Cas ) , Shc , and Nck , and activation of the extracellular regulated kinases ERK1 / 2 . ^^^ In RGD stimulated collagen embedded cells , FAK was phosphorylated only at Tyr 397 and c Src association occurred without Tyr 416 phosphorylation and p 130 ( Cas ) association . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Dominant negative studies indicate that Shc mediates the early phase and peak , whereas FAK , p 130 ( CAS ) , Crk , and Rap 1 contribute to the late phase of integrin dependent activation of ERK in these cells . ^^^ Although not necessary for signaling to ERK in primary fibroblasts , FAK may enhance and prolong integrin mediated activation of ERK through p 130 ( CAS ) , Crk , and Rap 1 in cells expressing B Raf . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Collagens or laminin , but not fibronectin , stimulated tyrosine phosphorylation of FAK , paxillin , and p 130 ( cas ) and activated ERK1 / 2 . ^^^ FAK , ERK , and p 130 ( cas ) tyrosine phosphorylation were activated after 10 min adhesion to collagen 4 . ^^^ FAK activity increased for 45 min after collagen 4 adhesion and persisted for 2 h , while p 130 ( cas ) phosphorylation increased only slightly after 10 min . ^^^ Transfection with FAK related nonkinase , but not substrate domain deleted p 130 ( cas ) , strongly inhibited ERK 2 activation in response to collagen 4 , indicating Caco 2 ERK activation is at least partly regulated by FAK . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These results indicate that CD 146 is coupled to a FYN dependent pathway that triggers Ca ( 2+ ) flux via phospholipase C gamma activation leading subsequently to the tyrosine phosphorylation of downstream targets such as Pyk 2 , p 130 ( Cas ) , FAK , and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In HUVECs stimulated with Sph 1 P , these data suggest the following : ( 1 ) cytoskeletal signalings may be separable into G ( 1 ) mediated signaling pathways ( involving Cas ) and Rho mediated ones ( involving FAK ) , and ( 2 ) coordinated signalings from both pathways are required for Sph 1 P enhanced HUVEC motility . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domain of CAS , when expressed in isolation from the rest of the protein , was able to target to focal adhesions , whereas a variant containing a point mutation that rendered the SH 3 domain unable to associate with FAK remained cytoplasmic . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Finally , we provide evidence that the Src dependent association of FAK with Grb 2 and p 130 ( Cas ) are both required for the regulation of cell cycle progression by FAK . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Analysis of focal adhesion kinase ( FAK ) , paxillin , and vinculin demonstrated disruption of focal adhesion complexes after 4 h of treatment with adenosine homocysteine followed by caspase induced proteolysis of FAK , paxillin , and p 130 ( CAS ) . ^^^ Pretreatment with the caspase inhibitor Z Val Ala Asp fluoromethylketone prevented adenosine homocysteine induced DNA fragmentation and FAK , paxillin , and p 130 ( CAS ) proteolysis . ^^^ Sodium orthovanadate did block adenosine homocysteine induced FAK , paxillin , and p 130 ( CAS ) proteolysis and Asp Glu Val Asp ase activity . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In vitro peptide binding experiments provide evidence that phosphorylation of pS 1 ( Ser 722 ) may play a role in modulating FAK binding to the SH 3 domain of the adapter protein p 130 ( Cas ) . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| PKC dependent activation of FAK and src induces tyrosine phosphorylation of Cas and formation of Cas Crk complexes . ^^^ The activity of two protein tyrosine kinases , Src and FAK , was shown to be necessary and sufficient for TPA induced Cas phosphorylation . ^^^ We propose that the PKC dependent phosphorylation of Cas by Src and FAK promotes the establishment of Cas Crk complexes and that these interactions may play an important role in regulating the actin cytoskeleton during neuronal differentiation . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| However , the induced expression of 14 3 3beta did not affect tyrosine phosphorylation of FAK or its substrates , p 130 ( cas ) and paxillin , suggesting that 14 3 3beta regulated integrin mediated cell spreading and migration by FAK independent mechanisms . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Such mutants , Crk Delta 255 or Crk Delta 242 Extended Linker ( Crk Delta 242 ( [ EL ] ) ) , characterized by a disruption in the SH 3 linker / C terminal SH 3 boundary , induced robust hyperphosphorylation of focal adhesion kinase ( FAK ) on Tyr ( 397 ) , hyperphosphorylation of focal adhesion proteins p 130 ( cas ) and paxillin and increased focal adhesion formation in NIH3T3 cells . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion kinase ( FAK ) and cell adhesion kinase beta ( CAKbeta ) / PYK2 / CADTK / RAFTK are protein tyrosine kinases that can colocalize with , bind to , and induce tyrosine phosphorylation of p 130 ( CAS ) and paxillin . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Collagen gel overlay induced apoptosis was accompanied by selective proteolysis of focal adhesion kinase ( FAK ) , talin , p 130 ( cas ) , and c src . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Two focal adhesion family kinases , Pyk 2 and , to a lesser extent , FAK were inducibly phosphorylated , as was a potential substrate , Cas . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Calyculin A induces focal adhesion assembly and tyrosine phosphorylation of p 125 ( Fak ) , p 130 ( Cas ) , and paxillin in Swiss 3T3 cells . ^^^ Thus , calyculin A induces transient focal adhesion assembly and tyrosine phosphorylation of p 125 ( Fak ) , p 130 ( Cas ) , and paxillin , acting downstream of ROK . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| A rapid increase in tyrosine phosphorylation of focal adhesion kinase ( FAK ) , paxillin , and Crk associated substrate ( CAS ) are prominent early events triggered by many G protein coupled receptors ( GPCRs ) , but the mechanisms involved remain unclear . ^^^ Here , we examined whether the Rho associated protein serine / threonine kinase family ( ROCK ) is a critical Rho effector in the pathway that links GPCR activation to the tyrosine phosphorylation of FAK , CAS , and paxillin . ^^^ HA 1077 , a preferential inhibitor of ROCK activity structurally unrelated to Y 27632 , also attenuated the increase in the tyrosine phosphorylation of FAK and paxillin but did not affect the tyrosine phosphorylation of CAS induced by bombesin in Swiss 3T3 cells . ^^^ The results demonstrate that ROCK dependent tyrosine phosphorylation of FAK and paxillin can be dissociated from a ROCK independent pathway leading to tyrosine phosphorylation of CAS . . ^^^ |
|
| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The addition of Thy 1 to matrix bound astrocytes induced recruitment of paxillin , vinculin , and focal adhesion kinase ( FAK ) to focal contacts and increased tyrosine phosphorylation of proteins such as p 130 ( Cas ) and FAK . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Treatment of differentiated cells with 1 mM carbachol caused rapid increases in the tyrosine phosphorylation of focal adhesion kinase ( FAK ) , Cas , and paxillin . ^^^ The src family kinase selective inhibitor PP 1 reduced carbachol stimulated tyrosine phosphorylation of FAK , Cas , and paxillin by 50 to 75 % . ^^^ Thus , muscarinic receptors activate protein tyrosine phosphorylation in differentiated cells , and the tyrosine phosphorylation of FAK , Cas , and paxillin , but not ERK1 / 2 , is mediated by a src family tyrosine kinase activated in response to stimulation of muscarinic receptors . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Treatment of A 549 cells with FAK antisense ( ISIS 15421 ) but not a mismatched control ( ISIS 17636 ) oligonucleotide resulted in reduced EGF stimulated p 130 ( Cas ) Src complex formation , c Jun NH ( 2 ) terminal kinase ( JNK ) activation , directed cell motility , and serum stimulated cell invasion through Matrigel . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src . ^^^ CAS makes multiple interactions , direct and indirect , with the tyrosine kinases Src and focal adhesion kinase ( FAK ) , and as a result of this complexity , several plausible models have been proposed for the mechanism of CAS SD phosphorylation . ^^^ The objective of this study was to provide experimental tests of these models in order to determine the most likely mechanism ( s ) of CAS SD tyrosine phosphorylation by FAK and Src . ^^^ In vitro kinase assays indicated that FAK has a very poor capacity to phosphorylate CAS SD , relative to Src . ^^^ However , FAK expression along with Src was found to be important for achieving high levels of CAS tyrosine phosphorylation in COS 7 cells , as well as recovery of CAS associated Src activity toward the SD . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| A FRNK variant in which the direct interaction with 5 Crk associated tyrosine kinase substrate ( CAS ) was disturbed by point mutations still functioned as an inhibitor of FAK , suggesting that FRNK is unlikely to inhibit FAK by sequestering CAS . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Paxillin was also found to play a critical role in fibronectin receptor biology ex vivo since cultured paxillin null fibroblasts display abnormal focal adhesions , reduced cell migration , inefficient localization of focal adhesion kinase ( FAK ) , and reduced fibronectin induced phosphorylation of FAK , Cas , and mitogen activated protein kinase . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Fluoroaluminate stimulates phosphorylation of p 130 Cas and Fak and increases attachment and spreading of preosteoblastic MC3T3 E 1 cells . ^^^ The addition of fluoroaluminate during cell attachment to type 1 collagen further stimulated phosphorylation of p 130 Cas and of Fak . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Activated Src ( SrcY529F ) as well as activation of putative Src signaling mediators ( Fak , Cas , Crk / CrkL , C3G , and Rap 1 ) blocked the effect of FA Csk in a manner dependent on Rap 1 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In contrast , Src mutations in the SH 2 or SH 3 domain greatly reduced binding to FAK , Cas , and paxillin but had little effect on tyrosine phosphorylation or biological assays . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Cas , Fak and Pyk 2 function in diverse signaling cascades to promote Yersinia uptake . ^^^ Using a reconstitution approach in Fak ( / ) fibroblasts , we have been able to specifically address the interplay between Fak , Cas and Pyk 2 in this process . ^^^ We show that both Fak and Cas play roles in the Yersinia uptake process and that Cas can function in a novel pathway that is independent of Fak . ^^^ Fak dependent Yersinia uptake does not appear to involve Cas Crk signaling . ^^^ By contrast , Cas mediated uptake in the absence of Fak requires Crk as well as the protein tyrosine kinases Pyk 2 and Src . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In neonatal cardiac myocytes FAK , Cas and paxillin are located in sarcomeric Z lines , suggesting that the Z line is an important signaling locus in these cells . ^^^ Moreover , expression of the C terminal focal adhesion targeting domain of FAK both disrupted sarcomeric organization and interfered with the localization of endogenous Cas to Z lines . ^^^ These findings suggest that the association of FAK and Cas and the preservation of multiple protein interaction motifs of Cas are required for the correct assembly of sarcomeres in cardiac myocytes . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| EphrinA 1 induced cytoskeletal re organization requires FAK and p 130 ( cas ) . ^^^ EphA 2 , focal adhesion kinase ( FAK ) and p 130 ( cas ) were identified as the major ephrin dependent phosphotyrosyl proteins during the ephrin induced morphological changes . ^^^ Mouse embryonic fibroblasts ( MEFs ) derived from FAK ( / ) and p 130 ( cas / ) mice had severe defects in ephrinA 1 induced cell spreading , which were reversed after re expression of FAK or p 130 ( cas ) , respectively . ^^^ These data show that ephrinA 1 can induce cell adhesion and actin cytoskeletal changes in fibroblasts in a FAK and p 130 ( cas ) dependent manner , through activation of the EphA 2 receptor . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| PKC regulated myogenesis is associated with increased tyrosine phosphorylation of FAK , Cas , and paxillin , formation of Cas CRK complex , and JNK activation . ^^^ We showed that , during differentiation , downregulation of PKC expression results in increased tyrosine phosphorylation of FAK , Cas , and paxillin , concomitant with enhanced Cas CrkII complex formation , which leads to activation of JNK 2 . ^^^ But in proliferated muscle cells , PKC inhibition results in FAK and Cas tyrosine dephosphorylation . ^^^ Further , disruption of actin cytoskeleton by cytochalasin D prevents the activation of FAK and Cas as well as the formation of Cas CrkII complex stimulated by PKC downregulation during muscle cell differentiation . ^^^ Based on the above data , we propose that PKC downregulation results in enhanced tyrosine phosphorylation of FAK , Cas , and paxillin , thus promoting the establishment of Cas CrkII complex , leading to activation of JNK and that these interactions are dependent upon the integrity of actin cytoskeleton during muscle cell differentiation . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Using various FAK mutants , we found that the simultaneous bindings of Src and p 130 ( cas ) were required for FAK to potentiate cell transformation . ^^^ Expression of FAK related nonkinase , kinase deficient Src , or the Src homology 3 domain of p 130 ( cas ) , which respectively serve as dominant negative versions of FAK , Src , and p 130 ( cas ) , apparently reversed the transformed phenotypes of FAK overexpressed cells upon HGF stimulation . ^^^ Moreover , FAK overexpression was able to enhance HGF elicited signals , leading to sustained activation of ERK , JNK , and AKT , which could be prevented by the expression of the Src homology 3 domain of p 130 ( cas ) . ^^^ Taken together , our results indicate that the synergistic effect of FAK overexpression and HGF stimulation leads to cell transformation and implicate a critical role of p 130 ( cas ) in this process . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Analysis of two known p 130 ( Cas ) associated tyrosine kinases FAK and Src indicated that the regulation of tyrosine phosphorylation of FAK and Src are altered in the tumor cells . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Constitutively activated R Ras ( 38V ) dramatically enhanced focal adhesion kinase ( FAK ) and p 130 ( Cas ) phosphorylation upon collagen stimulation or clustering of the alpha2beta1 integrin , even in the absence of increased ligand binding . ^^^ Signaling events downstream of R Ras differed from integrins and K Ras , since pharmacological inhibition of Src or disruption of actin inhibited integrin mediated FAK and p 130 ( Cas ) phosphorylation , focal adhesion formation , and migration in control and K Ras ( 12V ) expressing cells but had minimal effect in cells expressing R Ras ( 38V ) . ^^^ Therefore , signaling from R Ras to FAK and p 130 ( Cas ) has a component that is Src independent and not through classic integrin signaling pathways and a component that is Src dependent . ^^^ R Ras effector domain mutants and pharmacological inhibition suggest a partial role for phosphatidylinositol 3 kinase ( PI3K ) , but not Raf , in R Ras signaling to FAK and p 130 ( Cas ) . ^^^ Our results suggest that R Ras promotes focal adhesion formation by signaling to FAK and p 130 ( Cas ) through a novel mechanism that differs from but synergizes with the alpha2beta1 integrin . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In salmosin treated BCE cells , focal adhesion kinase ( FAK ) was dephosphorylated and expression of paxillin and p 130 ( CAS ) was decreased , but PI 3 kinase , ILK , and beta catenin were not expressed in decreased amounts or modified , suggesting that salmosin inactivated FAK dependent integrin signaling pathways . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of recombinant VCIP promoted adhesion , spreading and tyrosine phosphorylation of Fak , Shc , Cas and paxillin in endothelial cells . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Using Sertoli germ cells cocultured in vitro to study the regulation of AJ assembly , it was shown that this event associated with a transient induction of beta 1 integrin , vinculin , p FAK Tyr ( 397 ) , and phosphatidylinositol 3 kinase ( PI3K ) but not the nonphosphorylated form of focal adhesion kinase ( FAK ) , paxillin , and p 130 Cas . ^^^ When rats were treated with 1 ( 2 , 4 dichlorobenzyl ) indazole 3 carbohydrazide ( AF 2364 ) to perturb Sertoli germ cell AJs , an induction of beta 1 integrin , vinculin , p FAK Tyr ( 397 ) , PI3K , and p 130 Cas but not the nonphosphorylated form of FAK and paxillin was also detected in the testis , coinciding with the time spermatids began to deplete from the epithelium , indicating their involvement in AJ disassembly . ^^^ Thereafter , the levels of vinculin , p FAK Tyr ( 397 ) , PI3K , and p 130 Cas in the testis plunged , coinciding with the declining events of AJ disruption when virtually all spermatids were depleted from the epithelium . ^^^ In summary , the events of AJ dynamics in the testis , in particular at the site of ES , are regulated , at least in part , by proteins that are found in the FAC in other epithelia , such as beta 1 integrin , vinculin , and FAK utilizing the integrin / pFAK / PI3K / p130 Cas signaling pathway . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Transient overexpression of FRNK in SMC attenuated autophosphorylation of FAK at Tyr 397 , reduced Src family dependent tyrosine phosphorylation of FAK at Tyr 576 , Tyr 577 , and Tyr 881 , and reduced phosphorylation of the FAK / Src substrates Cas and paxillin . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| During cell spike formation , FAK and CAS were activated . ^^^ More CAS was activated in cells on fibrillar collagen gel than on the monomeric form , whereas FAK was activated to the same level on either . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The FcepsilonRI induced tyrosine phosphorylation of paxillin , Crk associated tyrosine kinase substrate ( CAS ) , and mitogen activated protein kinase proteins was independent of FAK . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion kinase ( FAK ) and p 130 ( Cas ) were phosphorylated when GD 25 alpha2beta1A cells , but not GD 25 alpha2beta1Amut cells were seeded on collagen coated dishes . ^^^ Subsequent treatment with PDGF BB further increased phosphorylation of FAK and p 130 ( Cas ) only in GD 25 alpha2beta1A cells . ^^^ However , when cultured within collagen lattices , FAK and p 130 ( Cas ) phosphorylation were stimulated in both alpha2beta1A and alpha2beta1Amut expressing cells but further phosphorylation , in response to subsequent treatment with PDGF BB , was seen only in GD 25 alpha2beta1A cells . ^^^ Phosphorylation of p 130 ( Cas ) , but not FAK , in GD 25 alpha2beta1Amut cells seeded in collagen lattices also depended on alphavbeta 3 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| An analysis of CAS and focal adhesion kinase ( FAK ) variants expressed in CAS and FAK deficient cell lines , respectively , indicated that CAS SD tyrosine phosphorylation is substantially achieved by Src family kinases brought into association with CAS through two distinct mechanisms : direct binding to the CAS Src binding domain and indirect association through a FAK bridge . ^^^ These findings further document a role for FAK as an important upstream regulator of CAS SD tyrosine phosphorylation and implicate CAS mediated signaling events in promoting membrane protrusion / lamellipodium extension during cell motility . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylation level of paxillin , a downstream target of FAK / Src , was unaffected by the beta 1 mutation , whereas tyrosine phosphorylation of CAS was strongly reduced . ^^^ The results demonstrate that CAS is a target for phosphorylation both by FAK dependent and independent pathways after integrin ligation . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In contrast to unaltered FAK / Src activity , the association of FAK and p 130 ( CAS ) was decreased in FAK Y861F transfected cells , and FAK phosphorylation at tyrosine 861 enhanced this association in vitro . ^^^ Taken together , these results strongly suggest that FAK phosphorylation at tyrosine 861 is crucial for H Ras induced transformation through regulation of the association of FAK with p 130 ( CAS ) . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| CAS expression led to a substantial increase in the phosphotyrosine content of FAK and paxillin , supporting a role for CAS as a positive regulator of Src activity at integrin adhesion sites . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Cas transmits signals through interactions with the Src homology 3 ( SH 3 ) and Src homology 2 domains of FAK or 5 Crk signaling molecules , or with 14 3 3 protein , as well as phosphatases PTP1B and PTP PEST . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We will identify the key players in the integrin mediated signaling pathways involved in cell motility and apoptosis , such as FAK , paxillin and p 130 ( CAS ) , and discuss how Src signaling affects the formation of focal adhesions and the extracellular matrix . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Using a constitutively active form for Mer ( CDMer ) or Gas 6 as a ligand to stimulate Mer , we found that Mer activation induced a post receptor signaling cascade involving Src mediated tyrosine phosphorylation of FAK on Tyr ( 861 ) , the recruitment of FAK ( Tyr 861 ) to the alphavbeta 5 integrin , and increased formation of p 130 ( CAS ) / CrkII / Dock180 complex to activate Rac 1 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domain of CAS mediates its interaction with several proteins involved in signaling pathways such as focal adhesion kinase ( FAK ) , tyrosine phosphatases PTP1B and PTP PEST , and the guanine nucleotide exchange factor C3G . ^^^ The structure enables modelling of the docking interactions to its ligands , for example from focal adhesion kinase , and supports structure based drug design of inhibitors of the CAS FAK interaction . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Here , it is shown that stimulation of human umbilical vein endothelial cells ( HUVEC ) with extracellular ATP or UTP increased intracellular free calcium ion concentrations ( [ Ca ( 2+ ) ] ( 1 ) ) , induced phosphorylation of focal adhesion kinase ( FAK ) , p 130 ( cas ) and paxillin , and caused cytoskeletal rearrangements with consequent cell migration . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We show that down regulation of PTP1B activity with small molecule inhibitors suppresses cell spreading and migration to fibronectin , increases Tyr ( 527 ) phosphorylation in Src , and decreases phosphorylation of FAK , p 130 ( Cas ) , and ERK1 / 2 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We recently reported that reexpression of CAS in CAS deficient mouse embryo fibroblasts transformed by oncogenic Src promoted an invasive phenotype associated with enhanced cell migration through Matrigel , organization of actin into large podosome ring and belt structures , activation of matrix metalloproteinase 2 , and elevated tyrosine phosphorylation of the focal adhesion proteins FAK and paxillin . ^^^ The ability of CAS to promote Matrigel invasion , formation of large podosome structures , and tyrosine phosphorylation of Src substrates , including FAK , paxillin , and cortactin , was also strictly dependent on the YxxP tyrosines . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Long term adhesion of endothelial cells to GST mLpp 3 RGE induced phosphorylation of FAK , SHC , and CAS , whereas adhesion to GST hLPP 3 RAD failed to do so . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition , under anoikis stress , the induction of the Y397F mutant in 5 Src transformed FAK ( / ) cells selectively led to a decrease in the level of p 130 ( Cas ) , but not other focal adhesion proteins such as talin , vinculin , and paxillin . ^^^ These results suggest that FAK may increase the susceptibility of 5 Src transformed cells to anoikis by modulating the level of p 130 ( Cas ) . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Under conditions of low concentrations of ECM , invasin was found to be the dominant adhesin , promoting high levels of phagocytosis coincident with robust and sustained activation of the protein tyrosine kinases Fak and Pyk 2 , phosphorylation of the adaptor molecule Cas and activation of the small GTPase Rac 1 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We show that Ang 2 interacts with alpha ( 5 ) beta ( 1 ) integrin in Tie 2 deficient human glioma cells , activating focal adhesion kinase ( FAK ) , p 130 ( Cas ) , extracellular signal regulated protein kinase ( ERK ) 1 / 2 , and c jun NH ( 2 ) terminal kinase ( JNK ) and substantially enhancing MMP 2 expression and secretion . ^^^ The Ang2 / alpha ( 5 ) beta ( 1 ) integrin signaling pathway was attenuated by functional inhibition of beta ( 1 ) and alpha ( 5 ) integrins , FAK , p 130 ( Cas ) , ERK1 / 2 , and JNK . ^^^ These data establish a functional link between Ang 2 interaction with alpha ( 5 ) beta ( 1 ) integrin and glioma cell invasion through the FAK / p130 ( Cas ) / ERK1 / 2 and JNK mediated signaling pathway . . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Cas proteins are large multidomain molecules that transmit signals as intermediaries through interactions with signaling molecules such as FAK and other tyrosine kinases , as well as tyrosine phosphatases . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| G treatment and was associated with a decrease of FAK association with adapter and cytoskeletal proteins , p 130 ( Cas ) and paxillin , respectively . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| CCK stimulated an association of Lyn with PKC delta , Shc , p 125 ( FAK ) and PYK 2 as well as with their autophosphorylated forms , but not with Cbl , p 85 , p 130 ( CAS ) or ERK 1 / 2 . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These alterations were coupled to increased Fak Tyr ( 397 ) autophosphorylation and to inhibition of Fak Tyr ( 925 ) , p 130 ( CAS ) , and paxillin phosphorylation . ^^^ An increased association of total Src with Fak and a decreased interaction of p 130 ( CAS ) and p 85 PI3K with Fak were also observed . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Furthermore , NRG 1Beta stimulated the formation of a multiprotein complex between erbB 2 , FAK , p 130 ( CAS ) and paxillin within 30 min , and induced lamellipodia with longitudinal elongation of the myocytes within days . ^^^ |
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| Interacting proteins: P56945 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Concordant with these findings , there was decreased interaction between FAK and its downstream partners p ( 130 ) Cas and Crk observed in FL cells but not in dPXXP cells . ^^^ |
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