| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.91749508 |
| Here we investigated whether direct interaction between uPAR , a glycosyl phosphatidylinositol anchored protein , and LRP , a transmembrane receptor , is required for clearance of uPA : PAI 1 , regeneration of unoccupied uPAR , activation of plasminogen , and the ability of HT 1080 cells to invade extracellular matrix . 0.91749508^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| It has been posited that the GPI anchor facilitates clearance of uPAR bound complexes between two chain urokinase ( tcuPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) by the alpha 2 macroglobulin receptor ( alpha 2MR ) which permits re expression of unoccupied uPA receptors on the cell surface . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Internalization and degradation require binding to uPAR and subsequently an interaction with the alpha 2 macroglobulin receptor ( alpha 2 MR ) . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Both LB 6 clone 19 cells , a mouse cell line transfected with the human uPAR cDNA , and the human U 937 monocytic cell line , express in addition to uPAR also the endocytic alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( LRP / alpha 2 MR ) which is required to internalize uPAR bound uPA PAI 1 and uPA PN 1 complexes . ^^^ Downregulation of cell surface uPAR molecules in U 937 cells was detected by cytofluorimetric analysis after uPA PAI 1 and uPA PN 1 incubation for 30 min at 37 degrees C ; this effect was blocked by preincubation with the ligand of LRP / alpha 2 MR , RAP ( LRP / alpha 2 MR associated protein ) , known to block the binding of the uPA complexes to LRP / alpha 2 . ^^^ Immuno electron microscopy confirmed the plasma membrane to intracellular translocation of uPAR , and its dependence on LRP / alpha 2 MR in LB 6 clone 19 cells only after binding to the uPA PAI 1 complex . ^^^ We conclude therefore that in the process of uPA serpin internalization , uPAR itself is internalized , and that internalization requires the LRP / alpha 2 MR . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| We have analyzed uPAR expression in nine leukemic cell lines of distinct lineages and maturational states and correlated this with expression of plasminogen receptors , tissue type plasminogen activator ( tPA ) receptors and LDL receptor related protein ( LRP ) . ^^^ Plasminogen and tPA receptors were expressed by all leukemic cell lines and by all nucleated peripheral blood cells but B and T lymphocytes were negative for cell surface expression of both uPAR and LRP while monocytes and neutrophils were positive for expression of both uPAR and LRP . ^^^ PMA stimulation induced surface expression of uPAR in lymphocytes but did not induce expression of LRP by these cells . ^^^ The comparison of uPAR expression between these cell lines and peripheral blood cells and it correlation with plasminogen receptors , tPA receptors and LRP expression offers new insights regarding potential mechanisms for regulation of uPA uPAR mediated pericellular proteolysis . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Low density lipoprotein receptor related protein ( LRP ) mediates the endocytosis of diverse ligands , including urokinase plasminogen activator ( uPA ) and its receptor , uPAR , which have been implicated in cellular migration . ^^^ These studies demonstrate alterations in cellular migration and in the activity of the uPA / uPAR system which accompany complete deficiency of LRP expression in fibroblasts . ^^^ We propose that uPA and uPAR form an autocrine loop for promoting fibroblast migration and that LRP counteracts the activity of this system . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the presence of both LRP and urokinase type plasminogen activator receptor ( uPAR ) in astrocytoma tissues and in glioma cell lines by PCR and immunohistochemical analysis . ^^^ LRP mRNA was expressed frequently in glioblastomas , as compared with low grade astrocytomas by PCR analysis and was well correlated with uPAR expression . ^^^ These results indicate that LRP is overexpressed in malignant astrocytomas , especially in glioblastomas , and the increased expression of LRP appears to correlate with the expression of uPAR and the malignancy of astrocytomas . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Internalization of the urokinase type plasminogen activator ( uPA ) requires two receptors , the uPA receptor ( uPAR ) and the low density lipoprotein receptor related protein ( LRP ) / alpha2 macroglobulin ( alpha2M ) receptor . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the presence of both LRP and urokinase type plasminogen activator receptor ( uPAR ) in glioblastoma by reverse transcriptase polymerase chain reaction ( RT PCR ) , and the cellular localization of LRP in glioblastoma tissues by immunohistochemical analysis . ^^^ LRP mRNA was frequently expressed in glioblastomas and anaplastic astrocytomas compared with low grade astrocytomas by RT PCR analysis , and was well correlated with uPAR expression . ^^^ These results indicate that LRP is present both in the cellular cytoplasm and on the cell surface of glioblastomas with an increased expression of uPAR . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The LRP deficient cells demonstrated increased levels of cell surface uPAR , higher levels of uPA in conditioned medium , increased migration on vitronectin coated surfaces , and increased invasion of Matrigel . ^^^ Antibodies which block binding of endogenously produced uPA to uPAR reduced ERK phosphorylation and migration of LRP deficient cells to the levels observed with control cells . ^^^ By regulating the uPA / uPAR system , LRP may also regulate ERK activation , cellular migration , and invasion . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The physiological internalization of urokinase inhibitor complexes is triggered by the interaction of plasminogen inhibitors ( PAIs ) with receptors belonging to the low density lipoprotein related receptor protein ( LRP ) family , and involves a macro quaternary structure including uPAR , LRP , and PAIs . ^^^ However , in contrast to this mechanism , we observed a two step process : first , the urokinase receptor ( uPAR ) acts as the anchoring factor on the plasma membrane ; subsequently , LRP acts as the endocytic trigger . ^^^ Once the chimera is bound to the plasma membrane by interaction with uPAR , we suggest that a possible exchange may occur to transfer the toxin to LRP via the saporin moiety and begin the internalization . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Involvement of the [ uPAR : uPA : PAI 1 : LRP ] complex in human myogenic cell motility . ^^^ At the cell surface , receptor ( uPAR ) bound urokinase ( uPA ) binds its inhibitor PAI 1 , localized in the matrix , and the complex is internalized by endocytic receptors , such as the low density lipoprotein receptor related protein ( LRP ) . ^^^ Using a two dimensional motility assay and microcinematography , we showed that any interference with the [ uPAR : uPA : PAI 1 ] complex formation , and interference with LRP binding to this complex , markedly decreased myogenic cell motility . ^^^ Inhibition of cell motility was associated with suppression of both filopodia and membrane ruffling activity . [ uPAR : uPA : PAI 1 : LRP ] complex formation involves high affinity molecular interactions and results in quick internalization of the complex . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| PAI 1 ( plasminogen activator inhibitor 1 ) binds the urokinase type plasminogen activator ( uPA ) and causes its degradation via its receptor uPAR and low density lipoprotein receptor related protein ( LRP ) . ^^^ The inhibitory effect of PAI 1 on uPA dependent chemotaxis is reversed when uPAR internalization is inhibited by the 39 kDa receptor associated protein or by anti LRP antibodies . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The present study was undertaken to investigate the expression of the molecules of the PA system ( tPA , uPA , PAI 1 , uPAR , LRP ) , as well as several members of the MMP family and their inhibitors in the course of actively induced EAE in BALB / c mice . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The urokinase type plasminogen activator ( uPA ) system is a dynamic complex in which the membrane receptor uPAR binds uPA that binds the plasminogen activator inhibitor ( PAI ) 1 localized in the extracellular matrix , resulting in endocytosis of the whole complex by the low density lipoprotein receptor related protein ( LRP ) . ^^^ We previously reported a nonproteolytic role of the [ uPAR : uPA : PAI 1 : LRP ] complex operative in cell migration . ^^^ We showed that the uPA system and LRP are localized at filopodia of invasive cells , and that formation / internalization of the [ uPAR : uPA : PAI 1 : LRP ] complex is required for attachment and migration of cancer cells on plastic and on a PAI 1 coat . ^^^ Migration velocity , expression of the uPA system , use of the [ uPAR : uPA : PAI 1 : LRP ] complex to migrate , and promigratory effects of PAI 1 paralleled cancer cell invasiveness . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The low density lipoprotein receptor related protein ( LRP 1 ) binds and mediates the endocytosis of multiple ligands , transports the urokinase type plasminogen activator receptor ( uPAR ) and other membrane proteins into endosomes , and binds intracellular adaptor proteins involved in cell signaling . ^^^ In this paper , we show that in murine embryonic fibroblasts ( MEFs ) and L 929 cells , LRP 1 functions as a major regulator of Rac 1 activation , and that this activity depends on uPAR . ^^^ In uPAR negative cells , LRP 1 neutralization does not affect Rac 1 activation , and other mechanisms by which LRP 1 may regulate cell migration are not unmasked . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| The aim of this work was to evaluate by immunohistochemistry ( IHC ) the expression of both LRP 1 and urokinase type plasminogen activator receptor ( uPAR ) at different developmental stages of rat prostate disease by using a prostate cancer model previously developed in our laboratory . ^^^ The IHC for uPAR in normal prostates and in premalignant lesions showed a score of immunostaining that correlated with the expression of LRP 1 . ^^^ On the other hand , in prostates with adenocarcinomas and undifferentiated carcinomas , LRP 1 was undetectable or weakly detected , whereas uPAR showed a significantly higher level of expression . ^^^ Based on the IHC results in rat prostates with premalignant and malignant lesions and considering that LRP 1 , by mediating the internalization of uPAR , is involved in the regulation of extracellular matrix remodeling and cell migration , we conclude that a decreased expression of LRP 1 could be involved with the increasing activation of plasminogen activators shown in cancers . . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| LR 11 colocalizes with uPAR on the cell surface and inhibits the LDL receptor related protein ( LRP ) mediated binding and internalization of uPAR . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Here we show that sorLA interacts with both components of the plasminogen activating system and PDGF BB similarly to LRP 1 ( LDL receptor related protein / alpha2 macroglobulin receptor ) , which is an important clearance receptor with established functions in controlling uPAR expression as well as PDGF BB signalling . ^^^ By using LRP 1 deficient cells transfected with sorLA , we demonstrate that sorLA bound ligand is internalized at a much lower rate than LRP 1 bound ligand , and that sorLA is inefficient in regulating cell surface uPAR expression , which depends on rapid internalization of the ternary complex between urokinase type plasminogen activator , its type 1 inhibitor , and uPAR . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| This study examined the role of uPAR and its co receptor LDL receptor related protein ( LRP ) in the regulation of kidney fibroblast proliferation and extracellular signal regulated kinase ( ERK ) signaling . ^^^ LRP protein was reduced and extracellular accumulation of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) proteins were greater in uPAR / cultures . ^^^ These data suggest that uPAR deficient kidney fibroblasts express lower levels of its scavenger co receptor LRP , resulting in greater extracellular accumulation of uPA and PAI 1 . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| Inhibition of endothelial cell movement and tubulogenesis by human recombinant soluble melanotransferrin : involvement of the u PAR / LRP plasminolytic system . ^^^ In addition , in hr sMTf treated HMEC 1 , the expression of both urokinase type plasminogen activator receptor ( u PAR ) and low density lipoprotein receptor related protein ( LRP ) are down regulated . ^^^ |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03405 and Q07954 |
Pubmed |
SVM Score :0.0 |
| NA |
|