| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Previous studies demonstrated that integrin alpha ( M ) beta ( 2 ) ( CD11b / 18 , Mac 1 ) forms a physical complex with the urokinase type plasminogen activator receptor ( uPAR / CD87 ) on leukocytes . ^^^ In this study , we used human peripheral blood neutrophils and transfected cells expressing alpha ( M ) beta ( 2 ) , uPAR , or both receptors to show that the integrin can directly interact with urokinase ( uPA ) . ^^^ Within the alpha ( M ) subunit of the integrin , the 1 domain interacts with uPA , which is distinct from the region that interacts with uPAR . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| The cell adhesion molecule integrin alphaMbeta 2 associates with the urokinase type plasminogen activator receptor ( uPAR ) on monocytes and neutrophils . uPAR also associates with members of the beta 1 and beta 3 integrins , and it modulates the ligand binding function of these integrins . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Neutrophil membranes were separated into lipid raft and non raft fractions , revealing the presence of uPAR and L selectin , but the virtual absence of CR 3 alpha chain in lipid rafts , either constitutively or in response to uPAR aggregation . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Mechanisms of pertussis toxin induced myelomonocytic cell adhesion : role of Mac 1 ( CD11b / CD18 ) and urokinase receptor ( CD 87 ) . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| The adhesive function of uPAR depends on a direct interaction with vitronectin which is increased by uPA and by modification of cell surface integrin ( such as CD11b CD 18 ) when associated to uPAR . ^^^ In this study we analysed the role of three deactivating cytokines , IL 4 , IL 10 and IL 13 , on the surface expression of uPA , uPAR and CD11b by monocytes and their consequences on monocyte adhesion to immobilized fibrinogen and vitronectin . ^^^ IL 10 induced a decrease in uPA and CD11b after 18 h incubation and a delayed decrease in uPAR which was only significant after 48 h incubation . ^^^ In contrast , IL 4 and IL 13 induced a decrease in uPAR after 18 h and a significant increase in uPA both in the cell lysates and at the cell surface , as well as an increase in cell surface associated CD11b . ^^^ This could be due to the decrease in uPAR because CD11b CD18 / uPAR forms a cell adhesion complex . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Here we investigated major adhesion molecules as predisposition factors via common polymorphisms in or in the vicinity of the candidate genes ICAM 1 , e selectin , PLAUR , CD11b , and CD 18 . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Flow cytometric analysis of surface antigen expression identified that after 5 days of culture the proliferating subpopulation of monocytes expressed CD 14 , CD 13 , CD 33 , CD11b , CD11c , CD 87 , HLA DR , CD45RO , and did not express CD 86 , CD 34 , CD 80 , CD 4 , CD 16 , and CD 56 . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| One such protein , the glycolipid anchored urokinase receptor ( uPAR ) , associates with and modifies the function of the beta ( 2 ) integrin Mac 1 ( CD11b / CD18 ) . ^^^ In this study , a critical non 1 domain binding site for uPAR on CD11b ( M 25 ; residues 424 440 ) is identified by homology with a phage display peptide known to bind uPAR . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Monoclonal antibodies ( mAbs ) directed against CD 14 , CD11b , CD 18 or urokinase receptor ( uPAR ) significantly inhibited PTX induced primed U 937 cell adhesion in serum in a concentration dependent manner . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Cross linking uPAR induced rapid and significant increases in membrane expression of CD11b and increased degranulation ( release of beta glucuronidase and lactoferrin ) to a significantly greater degree than cross linking control Abs . ^^^ We conclude that uPAR aggregation initiates activation signaling in polymorphonuclear neutrophils through at least two distinct uPA dependent and uPA independent pathways , increasing their proinflammatory potency ( degranulation and oxidant release ) and altering expression of CD11b / CD18 to favor a firmly adherent phenotype . . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| The mechanisms for these activities remain incompletely defined , although uPAR was recently identified as a cis acting ligand for the beta 2 integrin CD11b / CD18 ( Mac 1 ) . ^^^ Soluble uPAR bound to recombinant alpha3beta1 in a uPA dependent manner ( K ( d ) < 20 nM ) and binding was blocked by a 17 mer alpha3beta1 integrin peptide ( alpha 325 ) homologous to the CD11b uPAR binding site . uPAR colocalized with alpha3beta1 in MDA MB 231 cells and uPA ( 1 nM ) enhanced spreading and focal adhesion kinase phosphorylation on fibronectin ( Fn ) or collagen type 1 ( Col ) in a pertussis toxin and alpha 325 sensitive manner . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| The extensively glycosylated uPAR binds to the same C terminal lectin domain of CD11b that had previously been shown to prime Mac 1 / CR3 for cytotoxic degranulation in response to beta glucan . uPAR and beta glucan compete for a lectin site that is near to the CBRM1 / 23 epitope ( residues 943 1047 ) at the C terminus of CD11b , and thus the lectin domain is critical to both the adhesion and cytotoxic functions of Mac 1 / CR3 . ^^^ Adhesion is reversed when the uPA enzyme is captured by its receptor ( uPAR ) , causing uPAR to bind to CD11b at a second site ( residues 424 440 ) that is in between the N terminal 1 domain and the divalent cation binding region . . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| A single CD11b lectin site for beta glucan and uPAR was suggested because the binding of either beta glucan or uPAR to Mac 1 / CR3 selectively masked two CD11b epitopes adjacent to the transmembrane domain . ^^^ Moreover , treatment with phosphatidylinositol specific phospholipase C that removed GPI anchored proteins increased CD11b specific binding of ( 125 ) 1 labeled beta glucan by 3 fold and this was reversed with soluble recombinant uPAR . ^^^ Conversely , neutrophil activation for generation of Mac 1 / CR3 / uPAR complexes inhibited CD11b dependent binding of ( 125 ) 1 labeled beta glucan by 75 % . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| METHODS : Bone marrow aspirates from 11 control subjects were studied by flow cytometry and a lysed whole blood technique to compare surface expression of CD 87 on marrow granulocytes with those of CD11b , CD 16 , CD 35 , and CD 10 , which are expressed at the myelocyte , metamyelocyte , band , and segmented stage of neutrophilic development , respectively . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Double immunofluorescent staining of live human RPE cells with anti CR 3 antibody ( CD11b ) was performed to demonstrate the physical proximity of this beta 2 integrin with uPAR and determine whether associations were dependent on RPE confluence and polarity . ^^^ On elongated , live HRPE cells , uPAR dissociated from CD11b ( CR 3 ) and translocated to anterior poles of migrating cells . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Although uPAR was associated with CD11b ( CR 3 ) on live resting cells , polarized migratory HRPE cells were found to dissociate uPAR from CR 3 ; uPAR then translocated to anterior pole of the cell , where it enhanced PAI 1 inhibitable local proteolytic activity . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Mac 1 ( CD11b / CD18 ) and the urokinase receptor ( CD 87 ) form a functional unit on monocytic cells . ^^^ The leukocyte integrin Mac 1 ( CD11b / CD18 ) and the urokinase receptor ( uPAR , CD 87 ) mediate complementary functions in myelomonocytic cells . ^^^ Both receptors promote degradation of fibrin ( ogen ) and also confer adhesive properties on cells because Mac 1 and uPAR bind fibrin and vitronectin , respectively . ^^^ Because of the parallel roles and physical proximity of Mac 1 and uPAR , the capacity of these receptors to functionally interact was explored . ^^^ Induction of Mac 1 and uPAR expression on monocytic cell lines by transforming growth factor beta 1 and 1 . 25 ( OH ) 2 vitamin D 3 conferred urokinase and uPAR dependent adhesion to vitronectin , which was further promoted by engagement of Mac 1 . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Evidence in vitro has implicated uPAR as a modulator of beta 2 integrin function , particularly CR 3 ( CD11b / CD18 , Mac 1 ) . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Proteolytic cleavage of single chain , high molecular weight kininogen ( HK ) by kallikrein releases the short lived vasodilator bradykinin and leaves behind a two chain , high molecular weight kininogen ( HKa ) reported to bind to the beta 2 integrin Mac 1 ( CR 3 , CD11b / CD18 , alphaMbeta 2 ) on neutrophils and exert antiadhesive properties by binding to the urokinase receptor ( uPAR ) and vitronectin . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Expression of uPAR correlated with activation of beta ( 2 ) integrins lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) , measured by using monoclonal antibodies ( mAbs ) 24 and CBRM1 / 5 . ^^^ Isolated mononuclear cells ( MNCs ) from patients with AMI showed enhanced adhesiveness to human umbilical vein endothelial cells ( HUVECs ) , to fibrinogen ( Mac 1 ligand ) , and to vitronectin ( uPAR ligand ) . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| Regulation of CD11b / CD18 ( Mac 1 ) adhesion to fibrinogen by urokinase receptor ( uPAR ) . ^^^ OBJECTIVE AND DESIGN : The goal of this study is to investigate the consequence of the interaction between Mac 1 and uPAR and determine the mechanisms by which uPAR regulates Mac 1 dependent adhesion to fibrinogen . ^^^ MATERIAL : Human embryonic kidney 293 cells transfected with Mac 1 or uPAR or co transfected with both Mac 1 and uPAR . ^^^ RESULTS : The adhesion to fibrinogen was increased two fold for Mac 1 uPAR co transfected cells comparing to the Mac 1 transfected cells alone . ^^^ Phosphorylation of focal adhesion kinase ( FAK ) and mitogen activated protein kinase ( MAPK ) was increased in Mac 1 uPAR co transfected cells but not in Mac 1 transfected cells . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| High molecular weight kininogen fragments stimulate the secretion of cytokines and chemokines through uPAR , Mac 1 , and gC1qR in monocytes . ^^^ Antibodies to HK receptors on leukocytes including Mac 1 , LFA 1 , uPAR , and C1qR inhibited IL 1beta secretion induced by tKa 98 % , 89 % , 85 % , and 62 % , respectively . ^^^ |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11215 and Q03405 |
Pubmed |
SVM Score :0.0 |
| NA |
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