Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.69611729
At the level of the ER , caveolin 2 interacts with caveolin 1 to form a high molecular mass hetero oligomeric complex that is targeted to lipid rafts and drives the formation of caveolae . 0.69611729^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.51789852
More importantly , we show that caveolin 2 is phosphorylated in vivo at two serine residues and that the phosphorylation of caveolin 2 is necessary for its actions as a positive regulator of caveolin 1 during organelle biogenesis in prostate cancer cells . 0.51789852^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.70437865
First , using caveolin 1 deficient mouse embryonic fibroblasts , in which we have stably expressed caveolin 1 , 2 , or 3 , we find that caveolin 1 interacts with caveolin 2 in this setting , whereas caveolin 3 does not , in agreement with most published observations . 0.70437865^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.66575799
Cav 2 was able to interact with Cav 1 in the Golgi complex but this interaction was not sufficient to export it from this compartment . 0.66575799^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Here , an approximately 20 kDa caveolin related protein , caveolin 2 , was identified through microsequencing of adipocyte derived caveolin enriched membranes ; caveolin was retermed caveolin 1 . ^^^ Caveolins 1 and 2 are similar in most respects . mRNAs for both caveolin 1 and caveolin 2 are most abundantly expressed in white adipose tissue and are induced during adipocyte differentiation . ^^^ Caveolin 2 colocalizes with caveolin 1 , indicating that caveolin 2 also localizes to caveolae . ^^^ However , caveolin 1 and caveolin 2 differ in their functional interactions with heterotrimeric G proteins , possibly explaining why caveolin 1 and 2 are coexpressed within a single cell . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This appears to be a specific homo typic interaction , because the C terminal domain of caveolin 1 failed to interact with full length forms of caveolin 2 and caveolin 3 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Incubation of pure eNOS with peptides derived from the scaffolding domains of caveolin 1 and 3 , but not the analogous regions from caveolin 2 , resulted in inhibition of eNOS , inducible NOS ( iNOS ) , and neuronal NOS ( nNOS ) activities . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This pattern of caveolin 2 protein expression most closely resembles the cellular distribution of caveolin 1 . ^^^ In line with these observations , co immunoprecipitation experiments with mono specific antibodies directed against either caveolin 1 or caveolin 2 directly show that these molecules form a stable hetero oligomeric complex . ^^^ Despite this strict co localization , it appears that regulation of caveolin 2 expression occurs independently of the expression of either caveolin 1 or caveolin 3 as observed using two different model cell systems . ^^^ Although caveolin 1 expression is down regulated in response to oncogenic transformation of NIH 3T3 cells , caveolin 2 protein levels remain unchanged . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 1 is present on both the apical and basolateral plasma membrane , whereas caveolin 2 is enriched on the basolateral surface where caveolae are present . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In contrast , peptides derived from the caveolin 2 scaffolding domain and a caveolin 1 nonscaffolding domain had no effect . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We isolated a full length cDNA encoding human caveolin 3 , which is 72 % and 59 % homologous to human caveolin 1 and caveolin 2 , respectively . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
As this region ( 6 A2 / 7q31 ) is the site of an as yet unidentified tumor suppressor gene ( s ) , our mapping studies clearly define caveolin 1 and caveolin 2 as candidate genes that may be deleted at these loci . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Our current results show that mutational activation of c Neu down regulates caveolin 1 protein expression , but not caveolin 2 , in cultured NIH 3T3 and Rat 1 cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of caveolin 1 and 2 in differentiating PC 12 cells and dorsal root ganglion neurons : caveolin 2 is up regulated in response to cell injury . ^^^ In PC 12 cells , caveolin 1 expression is up regulated on day 4 of nerve growth factor ( NGF ) treatment , whereas caveolin 2 expression is transiently up regulated early in the differentiation program and then rapidly down regulated . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of caveolin 1 recruits caveolin 2 to caveolae membranes . ^^^ These results are consistent with the previous observation that in transformed NIH 3T3 cells that lack caveolin 1 expression , but continue to express caveolin 2 , no morphologically distinguishable caveolae are observed . ^^^ In direct support of this notion , regulated induction of caveolin 1 expression in transformed NIH 3T3 cells was sufficient to recruit caveolin 2 to caveolae membranes . ^^^ The ability of caveolin 1 to recruit caveolin 2 most likely occurs through a direct interaction between caveolins 1 and 2 , as caveolins 1 and 2 are normally co expressed and interact with each other to form high molecular mass hetero oligomers containing both caveolins 1 and 2 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Peptides from caveolin 2 exhibited no effect , whereas caveolin 1 and 3 stimulated activity 10 and 17 fold , respectively . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The human caveolin 1 gene contains three exons , while the human caveolin 2 gene contains two exons . ^^^ Interestingly , the boundary of the last exon of the human caveolin 1 and caveolin 2 genes are analogous , suggesting that they arose through gene duplication at this locus . ( CA ) n microsatellite repeat marker analysis of these caveolin genomic clones indicates they contain the marker D7S522 ( located at 7q31 . 1 ) , but not other microsatellite repeat markers tested . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Here , we employ an antisense approach to derive stable NIH 3T3 cell lines that express dramatically reduced levels of caveolin 1 but contain normal amounts of caveolin 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 expression is also up regulated in MCF 7 AdrR cells , but neither caveolin 1 nor caveolin 2 were detected in MCF 7 cells stably transfected with P gp . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The cellular distribution of caveolin 2 was similar to that of caveolin 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 1 , but not caveolin 2 , was upregulated in highly multidrug resistant SKVLBI cells that express high levels of P glycoprotein , and in low level Taxol resistant A 549 cell lines that express low amounts of P glycoprotein . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
There are multiple forms of caveolins : caveolin 1 and caveolin 2 are expressed as stable heterooligomeric complexes within most cell types , while caveolin 3 is restricted to striated muscle cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
With the exception of Calu 1 cell line , all cancer cell lines showed very low or no expression of caveolin 1 while caveolin 2 expression was similar to the one observed in normal bronchial epithelial cells . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Human caveolin 1 and caveolin 2 are closely linked genes colocalized with WI 5336 in a region of 7q31 frequently deleted in tumors . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
These GFP caveolin 1 fusion proteins co fractionated with endogenous caveolin 1 using an established protocol that separates caveolae derived membranes from the bulk of cellular membranes and cytosolic proteins , and co localized with endogenous caveolin 2 in vivo as seen by immunofluorescence microscopy . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Detergent solubility , oligomerization , and association with caveolin 2 were observed similarly for caveolin 1 in normal and 5 Src expressing cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Analysis of these contigs reveals that D7S522 is located approximately 67 kb upstream of the caveolin 2 gene and that the caveolin 2 gene is located approximately 19 kb upstream of the caveolin 1 gene , providing for the first time a detailed genetic map of this region . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This down regulation event was selective for caveolin 1 , as caveolin 2 levels remained constant under these conditions of growth factor stimulation . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
SW 13 cell lines contained little or no detectable caveolin 1 or caveolin 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We have expressed caveolin 1 in Caco 2 cells which do not express it and also caveolin 2 , a related protein of unknown function . ^^^ Expression of caveolin 1 led to formation of caveolae indicating that this protein is necessary for caveolae formation while caveolin 2 is restricted to the Golgi apparatus and has no effect on caveolae formation . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 localizes to the golgi complex but redistributes to plasma membrane , caveolae , and rafts when co expressed with caveolin 1 . ^^^ In Fischer rat thyroid ( FRT ) cells , which express low levels of caveolin 2 and no caveolin 1 , caveolin 2 localizes exclusively to the Golgi complex but is partially redistributed to the plasma membrane upon co expression of caveolin 1 by transfection or by adenovirus mediated transduction . ^^^ In Madin Darby canine kidney ( MDCK ) cells , which constitutively express both caveolin 1 and 2 , caveolin 2 localized to both the Golgi complex and to the plasma membrane , where it co distributed with caveolin 1 in flat patches and in caveolae . ^^^ In FRT cells , endogenous or overexpressed caveolin 2 did not associate with low density Triton insoluble membranes that floated in sucrose density gradients but was recruited to these membranes when co expressed together with caveolin 1 . ^^^ In MDCK cells , both caveolin 1 and caveolin 2 associated with low density Triton insoluble membranes . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of caveolin 1 is required for the transport of caveolin 2 to the plasma membrane . ^^^ Here , we show that caveolin 1 co expression is required for the transport of caveolin 2 from the Golgi complex to the plasma membrane . ^^^ We identified a human erythroleukemic cell line , K 562 , that expresses caveolin 2 but fails to express detectable levels of caveolin 1 . ^^^ This allowed us to stringently assess the effects of recombinant caveolin 1 expression on the behavior of endogenous caveolin 2 . ^^^ In addition , recombinant expression of caveolin 1 allows caveolin 2 to form high molecular mass oligomers that are targeted to caveolae enriched membrane fractions . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Immunoblot analysis revealed that particulate fractions from rat C 6 glioma cells express caveolin 1 and caveolin 2 . ^^^ Light density , Triton 10 100 insoluble caveolin 1 and caveolin 2 enriched fractions were obtained after fractionation of rat C 6 glioma cells and their separation over 5 40 % discontinuous sucrose density gradients . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Using an in vitro model of VSMC proliferation , we found that serum stimulation caused a dose dependent decrease in both caveolin 1 and caveolin 2 protein levels in human coronary artery smooth muscle cells . ^^^ Heparin , an inhibitor of VSMC proliferation , inhibited the serum induced loss of caveolin 1 and caveolin 2 . ^^^ In addition , heparin caused an increase in both caveolin 1 and caveolin 2 localization to caveolae enriched sucrose gradient membrane fractions when compared to serum alone . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Although caveolin proteins were long thought to be absent from the brain , we have shown that the human neuroblastoma cell line LAN 1 expresses both caveolin 1 and caveolin 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolar membrane PLD activity is stimulated 2 fold by low concentrations ( 10 30 microM ) of the caveolin 1 and caveolin 2 scaffolding domain peptides , whereas it is inhibited at higher concentrations of the peptides . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Genomic organization and transcriptional analysis of the human genes coding for caveolin 1 and caveolin 2 . ^^^ Caveolin 1 and caveolin 2 are related proteins involved in the biogenesis of caveolae . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Since anti caveolin antibodies and synthetic peptides derived from the scaffolding domains of caveolin 1 and caveolin 2 effectively attenuated second messenger responses in sensory cilia preparations in a characteristic manner , the data led to the suggestion that caveolins could mediate the assembly of signaling complexes within specialized membrane microdomains of olfactory sensory neurons . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Immunohistochemistry showed that caveolin 1 was mainly localized to caveolae in contractile cells and partly shifted to Golgi associated vesicles in synthetic cells , whereas caveolin 2 chiefly appeared in cytoplasmic vesicles in both cases . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In addition to caveolin 1 , the mRNA expression of caveolin 2 , a second member of the caveolin family , was also induced in keratinocytes after stimulation with KGF but also with other growth factors and cytokines . ^^^ In contrast to caveolin 1 , caveolin 2 protein was expressed in all layers of the normal epidermis and in the suprabasal layers of the hyperproliferative wound epithelium . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Coexpression of caveolin 1 and caveolin 2 caused more efficient formation of deep caveolae than caveolin 1 alone . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In addition , analysis of cultured fibroblasts from Cav 1 null embryos reveals the following : ( 1 ) a loss of caveolin 2 protein expression ; ( 2 ) defects in the endocytosis of a known caveolar ligand , i . e . fluorescein isothiocyanate albumin ; and ( 3 ) a hyperproliferative phenotype . ^^^ Thus , caveolin 1 expression is required to stabilize the caveolin 2 protein product , to mediate the caveolar endocytosis of specific ligands , to negatively regulate the proliferation of certain cell types , and to provide tonic inhibition of eNOS activity in endothelial cells . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The approximately 20 kDa protein was labelled specifically only by anti VIP21 / caveolin 1 , while the approximately 29 kDa protein was labelled by both anti VIP21 / caveolin 1 and anti caveolin 2 antibodies . ^^^ In elicited macrophages , caveolae ( labelled with anti VIP21 / caveolin 1 antibody ) appeared in large numbers on the cell surface , but caveolin detected by anti caveolin 2 was also found in small vesicles and multivesicular bodies . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Large numbers of caveolae , as well as elevated expression of caveolin 1 and caveolin 2 isoforms in the myometrium of ovariectomised ( OVX ) rats were detected . 17beta estradiol ( E 2 ) has a downregulating effect : the treatment of OVX rats with E 2 ( 5 microg / animal ) reduced the formation of caveolae by approx . 90 % . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The approximately 20 kDa protein was labelled specifically only by anti VIP21 / caveolin 1 , while the approximately 29 kDa protein was labelled by anti VIP21 / caveolin 1 and anti caveolin 2 . ^^^ In elicited macrophages , caveolae ( labelled with the anti VIP21 / caveolin 1 antibody ) appeared in large numbers on the cell surface , but caveolin detected by anti caveolin 2 was also found in small vesicles and multivesicular bodies in the cytoplasm . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Epithelial expression of caveolin 2 , but not caveolin 1 , is enhanced in the inflamed mucosa of patients with ulcerative colitis . ^^^ Caveolins are the principal structural component of caveolae membranes , and three distinct forms of caveolins have been identified : caveolin 1 , caveolin 2 , and caveolin 3 . ^^^ In this study , we evaluated the changes in the caveolin 1 and caveolin 2 expression in the inflamed mucosa of patients with IBD . ^^^ In normal colonic mucosa , caveolin 1 expression was detected in the smooth muscle cells of the muscularis mucosae and the endothelial cells , but caveolin 2 expression was not detected . ^^^ In the inflamed mucosa of patients with active UC , caveolin 2 expression was clearly detectable as small scattered foci on the luminal surfaces of epithelial cells , but caveolin 1 expression was similar to that in normal mucosa . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
RESULTS : The results of cDNA arrays showed that 13 cancer related genes were up regulated > or =2 fold ( CDC25B , cyclin D 1 , PCNA , MET , Jagged 2 , Integrin alpha 3 , Integrin alpha 6 , Integrin beta 4 , Caveolin 2 , Caveolin 1 , MMP 13 , MMP 14 , and BIGH 3 ) and 5 genes were down regulated > or =2 fold ( CK 4 , Bad , IGFBP 2 , CSPCP , and IL 1RA ) in both ESCC cell lines at the mRNA level . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of TARP in PC 3 cells resulted in a more rapid growth rate with a 5 h decrease in doubling time . cDNA microarray analysis of 6538 genes revealed that caveolin 1 , caveolin 2 , amphiregulin , and melanoma growth stimulatory activity alpha were significantly up regulated , whereas IL 1beta was significantly down regulated in PC 3 cells expressing TARP . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The addition of antibodies against caveolin 3 to the cell ' s cytoplasm via the pipette solution abrogated this direct G protein induced increase in sodium current , whereas antibodies to caveolin 1 or caveolin 2 did not . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Although caveolin 2 is coexpressed and heterooligomerizes with caveolin 1 in many cell types ( most notably adipocytes and endothelial cells ) , caveolin 2 has traditionally been considered the dispensable structural partner of the widely studied caveolin 1 . ^^^ In the absence of caveolin 2 protein expression , caveolae still form and caveolin 1 maintains its localization in plasma membrane caveolae , although in certain tissues caveolin 1 is partially destabilized and shows modestly diminished protein levels . ^^^ Our analysis of several different phenotypes observed in caveolin 1 deficient mice ( i . e . , abnormal vascular responses and altered lipid homeostasis ) reveals that Cav 2 null mice do not show any of these other phenotypes , indicating a selective role for caveolin 2 in lung function . ^^^ Taken together , our data show for the first time a specific role for caveolin 2 in mammalian physiology independent of caveolin 1 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
However , when fused to caveolin 2 , the OTR protein proved to be exclusively localized in caveolin 1 enriched fractions , where it bound the agonist with increased affinity and efficiently coupled to Galpha ( q / 11 ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The isoform lacking the C terminus localized to the endoplasmic reticulum , while the authentic caveolin 2 was distributed to the Golgi and the plasma membrane along with caveolin 1 . ^^^ The result confirmed the necessity of the C terminal domain of caveolin 2 for the caveolar localization , and showed the existence of a novel caveolin 2 isoform , which is not recruited to caveolae even in the presence of caveolin 1 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We also show that these mice are deficient in all three caveolin gene products , as caveolin 2 is unstable in the absence of caveolin 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In contrast to the results for caveolin 1 , in the case of caveolin 2 and 3 , chondrocytes were devoid of immunoreactivity in all regions of the epiphyseal cartilage . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Using immunochemical , immunocytochemical , and ultrastructural methods , we show that the placenta expresses caveolin 1 and caveolin 2 , which are marker proteins for caveolae . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of a caveolin mutant prevented the formation of the large caveolin 1 / 2 hetero oligomeric complexes , and led to intracellular retention of caveolin 2 and disappearance of caveolae from the basolateral membrane . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Two of three caveolin 2 antibodies show little basal staining , but strong signal throughout the rest of the epidermis , whereas a third shows a pattern like caveolin 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In this article , cholesterol transport to caveolin 1 and caveolin 2 containing compartments , such as the trans Golgi network ( TGN ) and plasma membrane caveolae , was examined in normal ( NPC+ / + ) , NPC heterozygous ( NPC+ / ) , and NPC homozygous ( NPC / ) human fibroblasts . ^^^ The expression of caveolin 1 and caveolin 2 was increased in NPC+ / and NPC / fibroblasts , although the distribution in each cell type was similar and characterized by predominant staining of the TGN and plasma membrane . ^^^ These studies demonstrate that NPC 1 regulates cholesterol transport to caveolin 1 and caveolin 2 containing compartments such as the TGN and plasma membrane caveolae . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 is a structurally related isoform that alone does not drive caveolae biogenesis ; rather , caveolin 2 cooperates with caveolin 1 to form caveolae in nonmuscle cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 1 and caveolin 2 , together with three bone morphogenetic protein related genes , may encode novel tumor suppressors down regulated in sporadic follicular thyroid carcinogenesis . ^^^ Three commonly down regulated genes , caveolin 1 , caveolin 2 , and GDF10 / BMP3b , were chosen for further study on the basis of their localization to two chromosomal regions , 7q31 . 1 and 10q11 . 1 , that commonly show loss of heterozygosity in FTC . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Peroxisome proliferator activated receptor gamma upregulates caveolin 1 and caveolin 2 expression in human carcinoma cells . ^^^ In human HT 29 colon carcinoma cells , thiazolidinedione PPARgamma ligands increased the levels of caveolin 1 and caveolin 2 proteins two to fivefold in a concentration dependent manner within 24 h . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of caveolin 1 and caveolin 2 in urothelial carcinoma of the urinary bladder correlates with tumor grade and squamous differentiation . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
BHK 1alpha cells exhibit a similar pattern of staining for caveolin 1 and caveolin 2 , and these two proteins show a high degree of co localization with mGlu1alpha receptor as demonstrated by immunogold and confocal laser microscopy . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In PT cells from adenomas , there are high levels of caveolin 2 , variably reduced caveolin 1 , and hyperactivation of ERK1 / 2 , which colocalizes with caveolin 1 in some cells , but localizes in the cytosol and nucleus in others . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We now show that endogenous caveolin 1 and caveolin 2 redistribute to LBs in lipid loaded A 431 and FRT cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolar microdomains isolated from BBCEC are enriched in P gp , cholesterol , caveolin 1 , and caveolin 2 . ^^^ Moreover , P gp interacts with caveolin 1 and caveolin 2 ; together , they form a high molecular mass complex . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Because hepatocytes express caveolin 1 and caveolin 2 , these proteins might modulate hepatic lipid metabolism and biliary lipid secretion . ^^^ C57BL / 6 mice were infected with recombinant human caveolin 1 and caveolin 2 adenoviruses . ^^^ Cav 2 infected mice exhibited a 10 and 7 fold increase in hepatic caveolin 1 and caveolin 2 protein expression , respectively . ^^^ Hepatic caveolin 1 and / or caveolin 2 overexpression significantly increased bile flow and secretion of all biliary lipids . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In normal MMCs , high levels of caveolin 2 and low level of caveolin 1 at mRNA and protein level were observed without any detectable expression of caveolin 3 . ^^^ Caveolae rich membranous fractions from the MMCs treated with Cd or SPER / NO contained both HO 1 and caveolin 1 or caveolin 2 . ^^^ The experiments of immuno precipitation showed complex formation between the HO 1 and caveolin 1 or caveolin 2 in the Cd treated MMCs . ^^^ Confocal microscopic results also support co localization of HO 1 and caveolin 1 or caveolin 2 at the plasma membrane . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Three caveolin isoforms are known : caveolin 1 , caveolin 2 and caveolin 3 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 associates with intracellular chlamydial inclusions independently of caveolin 1 . ^^^ METHODS : We utilized a caveolin 1 negative / caveolin 2 positive FRT cell line and laser confocal immunofluorescence techniques to visualize the colocalization of caveolin 2 with the chlamydial inclusions . ^^^ RESULTS : We show here that in infected HeLa cells , caveolin 2 , as well as caveolin 1 , colocalizes with inclusions of C . pneumoniae ( Cp ) , C . caviae ( GPIC ) , and C . trachomatis serovars E , F and K . ^^^ In addition , caveolin 2 also associates with C . trachomatis serovars A , B and C , although caveolin 1 did not colocalize with these organisms . ^^^ Using caveolin 1 deficient FRT cells , we show that although caveolin 2 normally is not transported out of the Golgi in the absence of caveolin 1 , it nevertheless colocalizes with chlamydial inclusions in these cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 1 alpha interacts with caveolin 1beta , caveolin 2 , actin , the microsomal form of NADH cytochrome B 5 reductase and ESA / reggie 1 / flotillin 2 as shown by co immunoprecipitation . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Clinical significance of Caveolin 1 , Caveolin 2 and HER2 / neu mRNA expression in human breast cancer . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Normally , cells that express caveolin 1 also express caveolin 2 , but this has not been demonstrated in bovine aortic endothelial cells ( BAECs ) . ^^^ Here , we show that BAECs express caveolin 2 , which localizes in caveolae with caveolin 1 . ^^^ We used differential gradient centrifugations and immunoprecipitation to show that bovine caveolin 2 and caveolin 1 form a hetero oligomer in plasma membrane caveolae . ^^^ Using immunocytochemistry we show that a pool of caveolin 2 also colocalizes with the cis Golgi in static culture , but unlike caveolin 1 , this Golgi associated pool is maintained after 1 day of shear exposure . ^^^ Therefore , the interaction of caveolin 2 with caveolin 1 could play an important role in caveolae biogenesis and shear stimulated mechano signal transduction . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Using TEM , immunocytochemistry and immunoblotting we show for the first time the existence of caveolae and the co localization of their signature marker integral proteins , caveolin 1 and caveolin 2 , in the intact lens of rabbit and guinea pig . ^^^ Immunofluorescence and immunoblot analyses confirmed the presence of caveolin 1 and caveolin 2 in the lens epithelium . ^^^ In addition , caveolin 1 and caveolin 2 co exist in the lens epithelium of both rabbit and guinea pig . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
An overlapping pattern of staining for mGlu1alpha receptor with caveolin 1 and caveolin 2 by confocal laser microscopy in transiently transfected HEK 293 cells is observed . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 is an accessory molecule and the binding partner of caveolin 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Western analysis demonstrated that mERhigh cells expressed caveolin 1 and caveolin 2 , and that ER alpha was contained in the same gradient separated membrane fractions . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The present study revealed : ( 1 ) Caveolae are most commonly observed in the Schwann cell plasmalemma of myelinated nerve fibers . ( 2 ) Caveolae are principally located in the perikaryal plasmalemma of Schwann cells of the myelinated nerves . ( 3 ) Caveolin 1 is expressed strongly in Schwann cell caveolae . ( 4 ) Caveolin 2 and 3 are also immunohistochemically detected in Schwann cell caveolae , although the immunoreactivities are slight . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In both nonpregnant and late pregnant myometrium , maxi K channels associate and colocalize with caveolar scaffolding proteins caveolin 1 and caveolin 2 , but not caveolin 3 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We have identified caveolin 1 ( alpha and beta ) , caveolin 2 and Cav 3 in the zebrafish . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In EAE lesions , the immunoreactivity of caveolin 1 was increased in the ependymal cells , some astrocytes , and some inflammatory cells of the spinal cord , while that of caveolin 2 showed an intense immunoreactivity . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
These findings suggest that rOAT 1 and caveolin 2 are co expressed in the plasma membrane and rOAT 1 ' s function for organic compound transport is upregulated by Cav 2 in the normal physiological condition . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 1 and caveolin 2 expression was much alike in control and pathological cases , while expression of caveolin 3 was lower in pathological samples . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
One of the major constituents of caveolae are three highly homologous caveolin isoforms ( caveolin 1 , caveolin 2 , and caveolin 3 ) . ^^^ Immunoblotting , conventional RT PCR , and real time RT PCR analysis established the expression of the caveolin 3 isoform in C 6 cells , in addition to caveolin 1 and caveolin 2 . ^^^ Western blot and real time RT PCR analysis demonstrate that caveolin 1 and caveolin 2 are up regulated during the late stages of the differentiation of C 6 cells . ^^^ In addition , co localization of caveolin 1 / caveolin 2 and caveolin 1 / caveolin 3 was detected in both C 6 glioma phenotypes . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This might be related to the nonpolar distribution of both caveolin 1 and 2 in RPE because caveolin 2 is basolateral and caveolin 1 nonpolar in other epithelial cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Expression of caveolin 1 and caveolin 2 in the human pituitary : an immunohistochemical study . ^^^ In the present work , expression of caveolin 1 and caveolin 2 was studied by immunohistochemistry using the streptavidin biotin peroxidase complex method . ^^^ No immunoreactivity was seen for caveolin 1 and caveolin 2 in non tumorous adenohypophysial and neurohypophysial cells and in the tumor cells , indicating that caveolins are not involved in the initiation and progression of pituitary adenomas . ^^^ The expression of caveolin 1 and caveolin 2 in the endothelial cells did not depend on age , gender , endocrine status of the patients , morphologic features , and type of pituitary tumors . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The expression of caveolin 1 as well as caveolin 2 and caveolin 3 was reduced . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolae are flask shaped endocytic structures composed primarily of caveolin 1 ( Cav 1 ) and caveolin 2 ( Cav 2 ) proteins . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We have also analyzed caveolin 1 gene expression and found that follows the opposite pattern of caveolin 2 , indicating that they are inversely regulated . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Caveolin 2 is coexpressed and hetero oligomerizes with caveolin 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Simvastatin partially restored caveolin 1 , caveolin 2 , and phospho caveolin expression in vessel walls . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
These caveolae contained caveolin 1 and caveolin 2 . ^^^ Another class of high density caveolae contained caveolin 1 , caveolin 2 and specifically fatty acid transport protein 1 , fatty acid transport protein 4 , fatty acyl CoA synthetase , hormone sensitive lipase , perilipin , and insulin regulated glucose transporter 4 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Monoclonal antibodies were produced against the Mirandola strain of canine adenovirus Type 1 ( CAV 1 ) and the Manhattan strain of canine adenovirus Type 2 ( CAV 2 ) . ^^^ Out of 36 monoclonal antibodies produced against the Mirandola strain , 18 were type specific for CAV 1 by IFA and 13 of those neutralized the virus in vitro . ^^^ The other 18 antibodies bound both CAV 1 and CAV 2 by IFA ; however , 7 of those specifically neutralized only CAV 1 . ^^^ The remaining 83 antibodies recognized both CAV 1 and CAV 2 by IFA , with 3 of those neutralizing both viral types . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This contrasted with our previous study ( CAV 1 protocol ) , which consisted of three courses of the same induction chemotherapy , the same intrathoracic irradiation , but with one year of oral maintenance chemotherapy . ^^^ During the CAV 1 regimen , we observed that herpes zoster developed in 13 of 161 ( 8 . 1 percent ) patients in association with their therapy . ^^^ The risk of development of herpes zoster in the CAV 1 group was significantly greater than the historical group ( p = 0 . 007 ) and was also greater than the CAV 2 group ( p = 0 . 031 ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The first consisted of six puppies , each of which was given two doses of a commercial inactivated CAV 1 vaccine , 14 days apart . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The putative protein has 80 % sequence similarity with the CAV 1 Fip . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
After resecting the epidural tumor of thoracic spine , chemotherapy ( CAV 1 kur ) and radiotherapy ( 4 , 200 rad ) was performed which was effective for minimizing the antero mediastinal tumor . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Using immunohistochemistry , we investigated the localization of constitutive neuronal ( nNOS ) and endothelial ( eNOS ) enzyme isoforms along with caveolin 1 ( CAV 1 ) and caveolin 3 ( CAV 3 ) throughout the systemic vasculature and peripheral tissues of the hamster . ^^^ In endothelial cells , eNOS and CAV 1 were present throughout the vasculature , whereas nNOS and CAV 3 were absent except in capillaries , which reacted for nNOS . ^^^ In smooth muscle cells , nNOS and CAV 1 were also expressed systemically , whereas eNOS was absent ; CAV 3 was present in the arterial but not the venous vasculature . ^^^ Both nNOS and CAV 3 were located at the sarcolemma of skeletal muscle fibers , which were devoid of eNOS and CAV 1 . ^^^ These immunolabeling patterns suggest functional interactions between eNOS and CAV 1 throughout the endothelium , regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle , and modulation of nNOS by CAV 3 in skeletal muscle . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Immunoprecipitation of Cav 1 from bovine lung microvascular endothelial cells shows that eNOS and hsp 90 are present in the Cav 1 complex . eNOS and hsp 90 from the lysate also interact with exogenous glutathione S transferase linked caveolin 1 ( GST Cav ) , and the addition of calcium activated calmodulin ( CaM ) to the GST Cav complex partially inhibited the association of eNOS and hsp 90 . ^^^ The association of eNOS with caveolin 1 ( Cav ) is believed to maintain eNOS in an inactive state ; however , increased association of eNOS to heat shock protein 90 ( hsp 90 ) is observed following activation . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NOS in canine lower esophageal sphincter : colocalized with Cav 1 and Ca2+ handling proteins . ^^^ The main Cav was Cav 1 . ^^^ In patch clamp studies , NO driven outward currents , mainly through BK ( Ca ) channels , were inhibited by antibodies to Cav 1 and not by calmodulin and were restored by an NO donor . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
We have used a combination of electron microscopy ( EM ) , circular dichroism , and analytical ultracentrifugation to determine the structure of the oligomers that form when the first 101 aa of caveolin 1 ( Cav ( 1 101 ) ) are allowed to associate . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Specifically , it was found that CAV 1 , 11 , 13 , 15 , 17 to 22 , and 24 are classified together with polioviruses and enterovirus 70 , whereas the rest of the CAVs are classified along with coxsackie B viruses , echoviruses , and the rest of the other enteroviruses . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Coronary allograft vasculopathy was defined as CAV 1 ( focal or diffuse stenosis < 50 % ) or CAV 2 ( focal or diffuse stenosis > or=50 % ) . ^^^ RESULTS : Results of coronary angiography were normal in 43 patients ( 63 % ) , showed CAV 1 in 11 ( 16 % ) , and showed CAV 2 in 14 ( 21 % ) . ^^^ After dipyridamole administration , wall motion remained normal in all 39 ( Group 1 , no CAV in 34 and CAV 1 in 5 ) . ^^^ Of 29 patients with rest wall motion abnormalities , all reversed to normal after dipyridamole in 8 patients ( Group 2 , no CAV in 7 and CAV 1 in 1 ) and remained or worsened in 21 ( Group 3 , CAV 2 in 14 and no CAV or CAV 1 in 7 ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In the 3CD region , CAV 1 , CAV 19 , and CAV 22 differed from one another by only 1 . 2 to 1 . 4 % and CAV 11 , CAV 13 , CAV 17 , CAV 20 , CAV 21 , CAV 24 , and the polioviruses differed from one another by only 1 . 2 to 3 . 6 % . ^^^ Only one group of serotypes ( CAV 1 , CAV 19 , and CAV 22 ) was consistently monophyletic in multiple genome regions . ^^^ The close relationship among the polioviruses and CAV 11 , CAV 13 , CAV 17 , CAV 20 , CAV 21 , and CAV 24 and the uniqueness of CAV 1 , CAV 19 , and CAV 22 suggest that revisions should be made to the classification of these viruses . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Recently , the scaffolding domain of caveolin 1 ( CAV ) has been implicated as a negative regulator of endothelial NOS ( eNOS ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
A peptide corresponding to the scaffolding domain of caveolin 1 ( Cav ( 82 101 ) ) dramatically inhibited cPLA 2 activity in purified hippocampal synaptoneurosomes . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Cav 1 containing lipid rafts and redistribution of Cav 1 , PKCgamma , and Cx 43 were analyzed by sucrose gradients and by consequent Western blot analysis . ^^^ RESULTS : Cav 1 and 2 were found in N / N1003A and bovine primary lens epithelial cells . ^^^ Cx 43 was associated with Cav 1 in lipid rafts . ^^^ Phorbol ester ( TPA ) and insulin like growth factor ( IGF ) 1 recruited PKCgamma into Cav 1 containing lipid rafts and stimulated the interactions of PKCgamma with Cav 1 and Cx 43 . ^^^ TPA and IGF 1 induced redistribution of Cav 1 and Cx 43 from light density fractions to higher density fractions on sucrose gradients . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
This lighter Golgi fraction was enriched for hypo oligomeric Cav 1 , indicating dysfunctional trafficking of cargo . ^^^ Immunofluorescence imaging studies confirmed the trapping of Cav 1 in a GM 130 positive Golgi compartment . ^^^ There was an increase in Ser 25 phosphorylation of GM 130 ( typically a prelude to Golgi fragmentation and mitosis ) and increased association between pGM 130 , cdc 2 kinase , and Cav 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Because only smooth muscle contains all caveolin ( Cav ) family members ( Cav 1 , 2 and 3 ) , we examined the contribution of each caveolin to urogenital smooth muscle structure / function . ^^^ MATERIALS AND METHODS : WT , Cav 1 , 2 , 3 and 1 / 3 knockout ( KO ) mouse bladders were characterized by Western blot , co immunoprecipitation , immunofluorescence microscopy , electron microscopy , histochemistry and pharmacological techniques . ^^^ RESULTS : The loss of bladder Cav 1 results in a marked decrease in Cav 2 but not Cav 3 expression . ^^^ Ablation of Cav 3 fails to alter Cav 1 or Cav 2 expression . ^^^ Deletion of Cav 1 results in the almost complete loss of caveolae , while Cav 2 KO and Cav 3 KO mouse smooth muscle showed a normal number of caveolae . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Application of the L ( Cav 1 ) type calcium channel antagonist nimodipine ( 10 microM ) had no effect . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
BACKGROUND : Caveolin 1 ( Cav 1 ) , a principal component of caveolae membranes , may function as a tumor suppressor in several types of human cancer . ^^^ RESULTS : We found two anchorage independent clones ( ST 1 and ST 3 ) that expressed reduced levels ( approximately 50 % ) of Cav 1 mRNA and protein , suggesting the inactivation of one allele of Cav gene . ^^^ However , haploinsufficiency of Cav 1 expression did not induce significant tumor formation when tested in nude mice . ^^^ CONCLUSION : Cav 1 haploinsufficiency in human breast epithelial cells can lead to partial transformation . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
MO targeting of anx2b or cav 1 , which are both strongly expressed in the larval and adult zebrafish intestinal epithelium , prevents formation of the protein heterocomplex . ^^^ These data suggest that ANX 2 and CAV 1 are components of an intestinal sterol transport complex . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Recently , the attention has been focused on the modulation of L channels ( CaV 1 ) , which represent the major Ca2+ current component in rat and human chromaffin cells . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In endothelia , NO is synthesized by endothelial NO synthase ( eNOS ) , which is negatively regulated by caveolin 1 ( Cav 1 ) , the primary coat protein of caveolae . ^^^ We show that delivery of Cav 1 amino acids 82 101 ( Cav ) fused to an internalization sequence from Antennapedia ( AP ) blocks NO release in vitro and inflammation and tumor angiogenesis in vivo . ^^^ To characterize the molecular mechanism by which the AP Cav peptide and Cav 1 mediate eNOS inhibition , we subdivided the Cav portion of AP Cav into three domains ( Cav A , B , and C ) , synthesized five overlapping peptides ( AP Cav A , AB , B , BC , and C ) , and tested their effects on eNOS dependent activities . ^^^ Mutation of F 92 to A 92 in the Cav 1 cDNA caused the loss of eNOS inhibitory activity compared with wild type Cav 1 . ^^^ These data highlight the importance of amino acids 89 95 and particularly F 92 in mediating eNOS inhibition by AP Cav and Cav 1 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Both mRNA and protein expression of NCX 1 , Cav 1 and Cav 2 was observed , but no expression of mRNA and protein of Cav 3 were observed in C 6 glioma cells . ^^^ In isolated caveolae enriched membrane fraction , the NCX 1 , Cav 1 and Cav 2 proteins localized in same fractions . ^^^ Functionally , sodium free induced forward mode of NCX 1 attenuated by Cav 1 antisense ODN . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In the isolated caveolae rich membrane fraction from Saos 2 cells , the CaSR , Cav 1 and Cav 2 proteins were localized in same fractions ( fraction number 4 and 5 ) . ^^^ The immuno precipitation experiment using the respective antibodies showed complex formation between the CaSR and Cav 1 , but no complex formation of CaSR and Cav 2 . ^^^ Confocal microscopy also supported the co localization of CaSR and Cav 1 at the plasma membrane . ^^^ Functionally , the [ Ca ( 2+ ) ] ( o ) induced [ Ca ( 2+ ) ] ( 1 ) increment was attenuated by the introduction of Cav 1 antisense oligodeoxynucleotide ( ODN ) . ^^^ From these results , in Saos 2 cells , the function of CaSR might be regulated by binding with Cav 1 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
RESULTS : In normal liver , scanty immunostaining for CAV 1 and 2 was observed in bile ductules . ^^^ In PBC liver samples , the expression levels of CAV 1 and 2 were increased on proliferating bile ductules especially in stage 3 cases , but was sparse on interlobular bile duct in stage 1 specimens . ^^^ Especially , the regenerating bile ductules at the interface of portal tracts and necrotic areas were immunostained intensely for CAV 1 and 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Canine distemper virus ( CDV ) , canine parvovirus type 2 ( CPV 2 ) , and canine adenovirus type 1 ( CAV 1 ) are pathogens that are typically associated with canids but may cause serious disease in a wide range of other carnivores . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In this study , using caveolin 1 null mice ( Cav ( / ) ) , we examined whether caveolin was also involved in the EPC recruitment in a model of ischemic hindlimb . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
To address the relationship of these two proteins , we investigated the protein protein interaction between rOAT 3 and Cav 1 . ^^^ The rOAT 3 mRNA and protein expression were observed in the rat kidney , and the expressions of Cav 1 mRNA and protein were also detected in the kidney . ^^^ Confocal microscopy of the immuno cytochemistry experiments using primary cultured renal proximal tubular cells showed that rOAT 3 and Cav 1 were co localized at the plasma membrane . ^^^ These findings suggest that rOAT 3 and caveolin 1 share a cellular expression in the plasma membrane and Cav 1 up regulates the organic anionic compound uptake via rOAT 3 under normal physiological conditions . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
METHODS : The renal distribution of rBAT 1 , rBAT and Cav 1 were demonstrated by employing reverse transcriptase polymerase chain reaction ( RT PCR ) and Western blot analysis . ^^^ Co localization of rBAT 1 and Cav 1 was observed by immunocytochemistry in primary cultured renal proximal tubule derived cells using a confocal microscope . ^^^ RESULTS : In the separated rat kidney tissues following the corticomedullary axis , Cav 1 mRNA and protein expressions were increased from the cortex to the inner medulla . rBAT 1 mRNA and protein expression were detected mainly in the outer medulla . ^^^ Confocal microscopic results showed rBAT 1 and Cav 1 co localization in the plasma membrane . ^^^ The effect of Cav 1 on rBAT 1 function was evaluated using Cav 1 antisense oligodeoxynucleotide ( ODN ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Here , we demonstrate that T lymphocytes express both regulatory beta 4 and poreforming Cav 1 alpha1 subunits of Cav channels . ^^^ Although Cav 1 channels of lymphocytes retain their voltage dependency , T cell receptor stimulation dramatically increases channel opening , providing a new mechanism for calcium entry in lymphocytes . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Twenty four Beagle pups with high levels of maternal antibody to canine adenovirus type 1 ( CAV 1 ) and canine adenovirus type 2 ( CAV 2 ) were oronasally inoculated with CAV 2 at 4 weeks of age . ^^^ In spite of the infection , maternal antibody continued to decrease for 4 to 8 postinoculation weeks , and then homologous CAV 2 neutralizing antibody and , to a lesser extent , CAV 1 neutralizing antibody began to increase . ^^^ When these pups were challenge inoculated with CAV 1 and CAV 2 at a time when maternal antibody to CAV 1 would normally have disappeared , they were immune . ^^^ In addition , 3 pups with maternal antibody to CAV 1 and CAV 2 were intramuscularly inoculated with CAV 2 at 3 weeks of age . ^^^ These pups were not immune to challenge inoculation with CAV 1 and CAV 2 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The Manhattan strain of canine adenovirus type 2 ( CAV 2 ) was examined . ^^^ Relatedness of CAV 2 DNA to the DNA of bovine adenovirus type 2 ( BAV 2 ) and human adenovirus type 2 ( HAV 2 ) has also been observed during DNA hybridization experiments . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Sequence analysis of a region extending between the pVIII and the fiber gene of Canine adenovirus type 1 ( Cav 1 , Utrecht ) and type 2 ( Cav 2 , Manhattan ) was performed . ^^^ The open reading frames of region E 3 in of Cav 1 and Cav 2 encoded a 22 kDa and 40 . 7 kDa polypeptide , respectively . ^^^ The Cav 1 and Cav 2 E 3 region polypeptides shared conserved amino and carboxyl domains . ^^^ It can be speculated that this insertion in the E 3 region contributes to the observed biological differences between Cav 1 and Cav 2 . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The level of homology observed across the E 1 regions appears to be of the same order as the overall DNA similarity between CAV 1 and CAV 2 ( 75 % ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
One isolate , 9228 , was selected as a prototype and was compared with reference strains of canine adenovirus 2 ( CAV 2 ) ( Manhattan and Toronto A26 / 61 ) and CAV 1 . ^^^ Isolate 9228 was clearly distinct from CAV 1 but identical to both Manhattan and Toronto ( A26 / 61 ) strains of CAV 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Immunity to canine adenovirus respiratory disease : a comparison of attenuated CAV 1 and CAV 2 vaccines . ^^^ One group was vaccinated with a live CAV 1 vaccine and another with a live CAV 2 vaccine . ^^^ In general , the degree of protection afforded by CAV 1 vaccine seemed similar to that provided by CAV 2 vaccine . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The fraction of dogs with CAV 1 antibody titres > or = 1 : 16 was significantly greater in the group that received inactivated CAV 1 vaccine than in the group vaccinated with attenuated live CAV 2 vaccine . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
All patients received two to four cycles of preoperative chemotherapy 4 every 3 weeks ( CAV 2 : cisplatin , 80 mg / m2 , day 1 ; doxorubicin hydrochloride ( Adriamycin ) , 30 mg / m2 , day 1 ; etoposide ( VePesid ) , 60 mg / m2 day 1 to 5 ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
As an initial trial to circumvent some of the shortcomings of HAV vectors , we have produced an E 1 deleted canine adenovirus type 2 ( CAV 2 ) vector for gene transfer . ^^^ Initially , we demonstrated that CAV 2 undergoes an abortive viral cycle in a wide range of human derived cell lines . ^^^ Second , we assayed human sera containing HAV 5 neutralizing antibodies for their ability to inhibit CAV 2 induced plaques on permissive cells . ^^^ In the cohort tested , our data demonstrate that the humoral response directed against HAV 5 does not inhibit CAV 2 plaque formation in the majority of cases . ^^^ Canine cell lines expressing the E 1 region of CAV 2 were generated and characterized . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Four strains of canine adenovirus type 1 ( CAV 1 ) and two strains of canine adenovirus type 2 ( CAV 2 ) were examined by restriction enzyme analysis . ^^^ In all cases , CAV 1 could be readily differentiated from CAV 2 . ^^^ Additionally , monoclonal antibodies were prepared against the Mirandola and Manhattan strains of CAV 1 and CAV 2 , respectively . 18 of 36 monoclonal antibodies from the CAV 1 fusion and 77 of 160 monoclonal antibodies from the CAV 2 fusion were type specific by an indirect fluorescent antibody technique . ^^^ Moreover , among those type specific monoclonal antibodies , 13 of the 18 CAV 1 antibodies and 39 of the 77 CAV 2 antibodies specifically neutralized only the homologous virus in vitro . ^^^ These data , along with other evidence from the literature , suggest that not only should CAV 1 and CAV 2 be recognized as distinct species in the genus mastadenovirus of the family Adenoviridae , but also that the major criterion of species distinction in that family , namely neutralization , should be reconsidered . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Both isolates , designated IAF 81 2116 and IAF 75 95 , appeared as genotypic variants of CAV 2 . ^^^ Seemingly , relatively small changes in the original CAV 2 DNA sequence allowed the virus to replicate at an unusual site in the dog . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
PROCEDURE : Dogs were > or = 2 years old and vaccinated against canine distemper virus ( CDV ) , canine adenovirus 1 ( CAV 1 ) , canine adenovirus 2 ( CAV 2 ) , canine parainfluenza virus ( CPIV ) , and canine parvovirus ( CPV ) . ^^^ Dogs were considered to have responded serologically if they had a day 0 serum neutralization titer to CDV > or = 1 : 32 ; a serum neutralization titer to CAV 1 , CAV 2 , or CPIV > or = 1 : 16 ; a hemagglutination inhibition titer to CPV > or = 1 : 80 ; or a > or = 4 fold increase in antibody titer after revaccination . ^^^ RESULTS : The percentage of dogs that had titers at or greater than the threshold values or responded to revaccination with a > or = 4 fold increase in titer was 98 . 1 % for CDV , 98 . 4 % for CAV 1 , 99 . 0 % for CAV 2 , 100 % for CPIV , and 98 . 1 % for CPV . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Of these genes , DSP , HMGA 1 ( alias HMGIY ) , TIMP 1 , MIF , CCNB 1 , TN , MMP 11 , and MMP 12 were upregulated and COPEB ( alias CPBP ) , TYROBP , BENE , BMPR 2 , SOCS 3 , TIMP 3 , CAV 1 , and CAV 2 were downregulated . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In addition , several genes , such as caveolin 1 ( CAV 1 ) and caveolin 2 ( CAV 2 ) , were constantly deregulated in all types of tumour sample , compared with normal tissue . ^^^ Immunostaining for CAV 1 was negative in 48 % of tumours , whereas 28 % of the tumours did not express CAV 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Phoneutria nigriventer omega phonetoxin IIA blocks the Cav 2 family of calcium channels and interacts with omega conotoxin binding sites . omega Phonetoxin IIA ( omegaPtxIIA ) , a peptide from spider venom ( Phoneutria nigriventer ) , inhibits high threshold voltage dependent calcium currents in neurons . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In the adult frog , Xenopus Cav 1 and Cav 2 are most abundantly expressed in the fat body and the lungs , while Xenopus Cav 3 is primarily expressed in muscle tissue types ( heart and skeletal muscle ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
FRA7G is a common fragile site containing the candidate tumor suppressor genes CAV 1 , CAV 2 , and TESTIN ( TES ) . ^^^ Our results demonstrate that CAV 1 , CAV 2 , and TESTIN are coamplified with the MET oncogene and overexpressed in GTL 16 . ^^^ The results show that CAV 1 , CAV 2 , and TESTIN are not tumor suppressor genes in this gastric cancer . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
According to the known functions of their genes , the clusters were designated as proliferating cell ( CDC 42 , TOP2A , FGFR 3 , MYC , etc . ) , neoangiogenesis and blood cell ( LCK , VAV 1 , KDR , VEGF , MMP 9 , SYK , PTPRS , and FLT 4 ) , invasion 1 and invasion 2 ( ADAM 17 , TRPM 2 , DUSP 6 , VIM , CAV 1 , CAV 2 , JAK 1 , PTPNS 1 , FYN , and PDGFB ) , HER 2 , and PSA / PSM / HER3 . ^^^ The results suggested that , along with PSA , PSM ( FOLH 1 ) , kallikrein 2 , and a 2 macroglobulin , cell signaling genes EGFR , HER 2 , HER 3 , TOP 2 , KRT 8 , KRT 18 , VEGF , CD 44 , VIM , CAV 1 , and CAV 2 may serve as diagnostic and prognostic markers in PC . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Alternative splicing of Cav 2 genes and their functional significance . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Deletion of CAV 1 does not result in either a compensatory increase of CAV 2 or CAV 3 in intestine . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The generation of mice deficient in the various caveolin genes ( cav 1 , cav 2 and cav 3 ) has provided physiological models to unravel the role of caveolins or caveolae at the whole organism level . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
The virus could be neutralised with a CAV 2 specific reference antiserum and also showed some cross neutralisation with CAV 1 specific reference antiserum . ^^^ The virus 5 113 87 had a high haemagglutination inhibition titre with CAV 2 antiserum using human group 0 red blood cells and CAV 1 and CAV 2 reference antisera . ^^^ This virus also had DNA restriction profiles identical to those of the reference CAV 2 ( Toronto A26 / 61 ) , whereas previously isolated strains of adenovirus from dogs in New Zealand had DNA restriction patterns identical to the prototype CAV 1 strain ( Utrecht ) . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Both immunoblot analysis and immunohistochemistry dramatically show that Cav 1 and Cav 2 expression is downregulated to almost undetectable levels in the lungs of post MI rats . ^^^ We also show that STAT 3 is hyperphosphorylated , and cyclin D 1 and D 3 levels are dramatically upregulated , in lung tissue samples derived from Cav 1 ( / ) and Cav 2 ( / ) deficient mice . ^^^ CONCLUSIONS : Thus , down modulation of pulmonary Cav 1 and Cav 2 expression in rats subjected to MI may represent an initiating mechanism leading to the activation of the STAT3 / Cyclins pathway and , ultimately , to the development of PH and lung structural remodeling . . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
PTCs showed overexpression of CITED 1 , claudin 10 ( CLDN 10 ) , and insulin like growth factor binding protein 6 ( IGFBP 6 ) but showed no change in expression of caveolin 1 ( CAV 1 ) or 2 ( CAV 2 ) ; conversely , FTCs did not express CLDN 10 and had decreased expression of IGFBP 6 and / or CAV 1 and CAV 2 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Mechanism of SNARE protein binding and regulation of Cav 2 channels by phosphorylation of the synaptic protein interaction site . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
In contrast , the genes CAV 1 , CAV 2 , and GNG 11 located in 7q31 , a commonly deleted area , were down regulated in the entire series . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
A reconfiguration of CaV 2 Ca2+ channel current and its dopaminergic D 2 modulation in developing neostriatal neurons . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Four nine to 11 week old puppies developed respiratory and neurological signs due to an infection with canine adenovirus type 2 ( cav 2 ) ; three of these were euthanased . ^^^ Adenovirus specific nucleic acid was detected by pcr in samples of lung and brain and the amplified product was 99 . 8 per cent homologous with the cav 2 reference strain Toronto a26 / 61 . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
METHODS : Western blotting , real time quantitative PCR analysis of abraded tracheal epithelium and laser assisted microdissection combined with subsequent mRNA analysis were used to examine the expression of cav 1 and cav 2 , two major caveolar coat proteins , in rat tracheal epithelium . ^^^ RESULTS : Western blotting and laser assisted microdissection identified protein and transcripts , respectively , of cav 1 and cav 2 in airway epithelium . ^^^ Real time quantitative RT PCR analysis of abraded tracheal epithelium revealed a higher expression of cav 2 than of cav 1 . ^^^ Immunoreactivities for cav 1 and for cav 2 were co localized in the cell membrane of the basal cells and basolaterally in the ciliated epithelial cells of large airways of rat and human . ^^^ However , no labeling for cav 1 or cav 2 was observed in the epithelial cells of small bronchi . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
Mechanistically , the development of PH was associated with reduced expression of pulmonary Cav 1 and Cav 2 , hyperactivation of the STAT 3 signaling cascade , and upregulation of cyclin D 1 and D 3 protein levels , all of which were prevented by administration of AP Cav . ^^^
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA
Interacting proteins: P51636 and Q03135	Pubmed	SVM Score :0.0
NA