| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.69696543 |
| In addition , a small amount of iNOS was found to cofractionate with cav 1 in Triton 10 100 insoluble membrane fractions where also iNOS degradation was apparent . 0.69696543^^^ As has been described for endothelial and neuronal NOS isoenzymes , direct binding between cav 1 and human iNOS was detected in vitro . 0.53117903^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.68478158 |
| Because activity of endothelial NOS ( eNOS ) is thought to be regulated by its interaction with the caveolar protein caveolin 1 , structural relationships between eNOS , caveolin 1 , and the VEGF receptor FIk 1 / KDR were analyzed with double label immunofluorescence and cell fractionation procedures . 0.68478158^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Interactions with caveolin 1 control the intracellular locale and degradation of iNOS in tumor cells . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In addition , NOS activity was negatively correlated to caveolin 1 level in the cytosolic fractions . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| A depletion of cholesterol in the caveolae induces a decrease in caveolin 1 at the cell surface allowing NOS activation . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Incubation of pure eNOS with peptides derived from the scaffolding domains of caveolin 1 and 3 , but not the analogous regions from caveolin 2 , resulted in inhibition of eNOS , inducible NOS ( iNOS ) , and neuronal NOS ( nNOS ) activities . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Tissue levels of connexins 40 , 43 ( major components of gap junction ) , inducible NOS ( iNOS ) , endothelial NOS ( eNOS ) and eNOS regulator proteins such as calmodulin , heat shock protein 90 ( hsp 90 ) and caveolin 1 were also examined using Western blot . 2 . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis was used to evaluate cytochrome c , endothelial and inducible NOS , NADPH oxidase , caveolin 1 and RhoA . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Subsequent 24 h or more reperfusion time led to an increase in inducible NOS ( iNOS ) expression and NO production , as well as a decline of caveolin 1 protein at the core and penumbra of the ischemic brain . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Protein expression of endothelial nitric oxide synthase ( eNOS ) , inducible NOS ( iNOS ) , caveolin 1 and PKC were determined . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Cav 1 ( / ) lungs concurrently showed suppression of NF kappaB activity and decreased transcription of inducible NO synthase and ICAM 1 . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| METHODS : We evaluated the expressions of caveolin 1 , caveolin 3 , and iNOS in 16 seminoma and 10 non seminoma testicular cancer specimens as well as normal testicular tissue , using a streptavidin biotin bridge technique on cryostat sections . ^^^ RESULTS : Immunohistochemistry revealed that caveolin 3 , but not caveolin 1 , was frequently expressed in seminomas ( 12 positive out of 16 specimens ) without positive staining in their normal counterparts or in nonseminomatous germ cell tumors , except for muscle components in the teratoma . iNOS was not expressed in any tissues examined . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In particular , we were interested in the possibility that caveolin 1 , a protein that functions as a tumor suppressor in colon carcinoma cells ( Bender et al . , 2002 ; this issue ) , might regulate iNOS activity . ^^^ Our results provide evidence for the existence of a post transcriptional mechanism controlling iNOS protein levels that involves caveolin 1 dependent sequestration of iNOS within a detergent insoluble compartment . ^^^ Interestingly , despite the high degree of conservation of the caveolin 1 scaffolding domain binding motif within all NOS enzymes , the interaction detected between caveolin 1 and iNOS in vitro is crucially dependent on presence of a caveolin 1 sequence element immediately adjacent to the scaffolding domain . ^^^ These observations are important in the context of tumor biology , since down regulation of caveolin 1 is predicted to promote uncontrolled iNOS activity , genotoxic damage and thereby facilitate tumor development in humans . . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Overexpression of an inactive mutant of Cav 1 lacking the tyrosine phosphorylation site ( Y14F ) or an siRNA mediated Cav 1 knock down also resulted in marked attenuation of IL 1beta induced iNOS gene expression and NO release from these cells , thus further implicating Cav 1 in this signaling cascade . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NOS activity correlated positively with calmodulin concentration in both of the fractions . nNOS and iNOS colocalized with caveolin 1 , as evidenced by immunocytochemical and immunoprecipitation studies . ^^^ The results thus provide first evidence of nNOS and iNOS in the nuclear compartment and suggest NOS interaction with caveolin 1 in rat PMNs . . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We conclude that : ( 1 ) baseline permeability does not depend on eNOS ; ( 2 ) eNOS and NO are integral elements of the signalling pathway for the hyperpermeability response to PAF ; ( 3 ) iNOS does not affect either baseline permeability or hyperpermeability responses to PAF ; and ( 4 ) caveolin 1 inhibits eNOS regulation of microvascular permeability in vivo . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In isolated cell systems , nitric oxide synthase ( NOS ) activity is regulated by caveolin ( CAV ) , a resident caveolae coat protein . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Neither the expression nor the interaction of caveolin 1 and caveolin 3 with NOS enzymes was found to be altered in muscle of diabetic rats . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Our results indicate that eNOS activity is up regulated in Cav 1 null animals , and this activity can be blunted by using a specific NOS inhibitor , nitro l arginine methyl ester . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| This study investigated the effects of an Ang 2 type 1 receptor antagonist , TCV 116 , on endothelial nitric oxide synthase ( eNOS ) mRNA and protein expression , and NOS activity and eNOS regulatory protein caveolin 1 protein expression in the left ventricle of Wistar Kyoto rats treated for 2 weeks with Ang 2 ( 200 ng / kg / min ) and evaluated these relations to myocardial remodeling . ^^^ The eNOS mRNA and protein levels , and NOS activity and caveolin 1 protein expression in the left ventricle were significantly decreased in ANGII compared with control rats ( CON ) , and were significantly increased in ANGII TCV compared with ANGII . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunogold labeling on samples of isolated perfused rat hearts embedded by an innovative low temperature LR White procedure provided detailed insight into the interaction of caveolin 1 and endothelial NOS in myocardial capillary endothelium at the subcellular level . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 coimmunoprecipitates with eNOS ; interaction with eNOS occurs via the caveolin 1 scaffolding domain and appears to result in the inhibition of NOS activity . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptors ( ER alpha and ER beta ) , NOS , heat shock proteins 70 and 90 ( HSP 70 and HSP 90 ) , caveolin 1 , 2 , and 3 , calmodulin , NOS activity , and cGMP were analyzed in a lysate of platelets from gonadally intact and ovariectomized female pigs . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical studies of dog saphenous vein using antibodies raised against neuronal NOS indicated prominent staining along the plasmalemma in a punctate pattern similar to the distribution of antibodies against caveolin 1 , a major constituent of the plasmalemmal caveolae . 5 . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Recently , the scaffolding domain of caveolin 1 ( CAV ) has been implicated as a negative regulator of endothelial NOS ( eNOS ) . ^^^ |
|
| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunoblot and immunohistochemistry results indicate the following : 1 ) LAD endothelial NOS protein content was similar among groups ; 2 ) HF decreased LAD superoxide dismutase ( SOD ) but increased caveolin 1 content ; and 3 ) Ex increased SOD content of HF LADs . ^^^ We conclude that HF impairs EDR by impairing the contribution of NO released from NOS ( due to decreased SOD and increased caveolin 1 protein content ) and by production of an Indo sensitive vasoconstrictor . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Cardiac and aortic expression of endothelial NOS and caveolin 1 were measured by immunoblotting . ^^^ Aortic caveolin 1 protein , an inhibitor of endothelial NOS , was also increased in these mice by 2 . 0 fold and correlated positively with systolic BPV in the very low frequency band . ^^^ CONCLUSIONS : Rosuvastatin decreases caveolin 1 expression and promotes NOS function in apoE / , dyslipidemic mice in vivo , with concurrent improvements in BPV and HRV . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| To explore the participation of nitric oxide ( NO ) and caveolin 1 in this protective effect , we evaluated proteinuria , creatinine clearance , renal structural lesions , nitrites and nitrates urinary excretion ( UNO ( 2 ) ( ) / NO ( 3 ) 5 ) , and mRNA and protein levels of neuronal NO synthase ( nNOS ) , endothelial NOS ( eNOS ) , and caveolin 1 in lean and fatty Zucker rats fed with 20 % casein or soy protein diet . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The present study was designed to test the hypothesis that CRF would induce changes in caveolin 1 ( Cav 1 ) , soluble guanylate cyclase ( sGC ) and Akt , three proteins important in regulating NO synthase ( NOS ) functionality . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We have demonstrated that inhibition of NO synthase ( NOS ) in endothelial cells by either the NOS inhibitor N ( omega ) monomethyl l arginine ( l NMMA ) or the internalization of caveolin 1 scaffolding domain attenuated platelet activating factor ( PAF ) induced increases in microvessel permeability ( Am J Physiol Heart Circ Physiol 286 : H 195 H201 , 2004 ) indicating the involvement of an NO dependent signaling pathway . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical staining showed loss of caveolin 1 and NOS in the jejunal smooth muscles and myenteric plexus interstitial cells of Cajal ( ICC ) of Cav 1 ( / ) mice ; however , nNOS immunoreactive nerves were still present in myenteric ganglia . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The study further sought to explore expressions of caveolin 1 , phospho AKt , and calmodulin , which regulate activities of constituitive NOS isoforms , as well as soluble guanylate cyclase ( sGC ) , which is involved in NO signaling . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Antagonizing ET 1 effects and blocking its activation in LPS pretreated SECs decreased the LPS induced overexpression of CAV 1 as well as the inhibition of ET 1 induced NOS activity . ^^^ LPS inhibits endothelin 1 induced endothelial NOS activation in hepatic sinusoidal cells through a negative feedback involving caveolin 1 . ^^^ |
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| Interacting proteins: P35228 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Likewise , muscle cells obtained from NOS 2 ( / ) knockout mice challenged with LPS / IFN gamma could downregulate their levels of cav 1 but not of cav 3 , unlike wild type animals , in which both cav 1 and cav 3 levels diminished in the presence of the proinflammatory insult . ^^^ When the induced NOS 2 was purified using an affinity resin or immunoprecipitated from muscular tissues , it appears strongly bound not only to calmodulin but also to cav 1 , and marginally to cav 2 and cav 3 . ^^^ When the cav levels where reduced using antisense oligonucleotides , an increase in the NOS 2 derived . ^^^ Our results show that cells expressing NOS 2 diminish their cav levels when the synthesis of * NO is required . . ^^^ |
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