| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.93410094 |
| In the present study we show that caveolin 1 binds directly to ERalpha . 0.93410094^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.5969027 |
| In human vascular smooth muscle or MCF 7 ( human breast cancer ) cell membranes , coimmunoprecipitation shows that ER associates with caveolin 1 and 2 . 0.5969027^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.65589153 |
| We found that palmitoylation of ERalpha enacts ERalpha association with the plasma membrane , interaction with the membrane protein caveolin 1 , and nongenomic activities , including activation of signaling pathways and cell proliferation ( i . e . , ERK and AKT activation , cyclin D 1 promoter activity , DNA synthesis ) . 0.65589153^^^ |
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| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| We found ER alpha in the nucleus and cell periphery , where one third colocalized with caveolin 1 . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 mutations in human breast cancer : functional association with estrogen receptor alpha positive status . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| We show that in addition to homooligomers of caveolin 1 , heterooligomeric complexes of caveolin 1 and 2 are formed in the ER . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 mediated activation of ERalpha was sensitive to a well known ER antagonist , 4 hydroxytamoxifen . ^^^ Caveolin 1 potentiates estrogen receptor alpha ( ERalpha ) signaling . caveolin 1 drives ligand independent nuclear translocation and activation of ERalpha . ^^^ Here , we show that caveolin 1 potentiates ERalpha mediated signal transduction . ^^^ Coexpression of caveolin 1 and ERalpha resulted in ligand independent translocation of ERalpha to the nucleus as shown by both cell fractionation and immunofluorescence microscopic studies . ^^^ Similarly , caveolin 1 augmented both ligand independent and ligand dependent ERalpha signaling as measured using a estrogen response element based luciferase reporter assay . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| The administration of the pure estrogen receptor ( ERalpha ) antagonist ICI 182 , 780 ( 1 mg / animal ) not only compensated for the inhibitory effect of E 2 , but further increased the level of caveolin 1 in the myometrium of OVX rats and facilitated the formation of caveolae by approximately 70 % . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| These increases in caveolin 1 were inhibited by the estrogen receptor antagonist ICI 182 , 780 ( 10 ( 6 ) M ) . ^^^ Results of this study suggest that E ( 2 ) stimulates caveolin 1 transcription and translation through estrogen receptor mediated mechanisms . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Moreover , some beta COP colocalizes with intracellular caveolin 1 , which was previously shown to be present on a new organelle ( termed the caveosome ) that is an intermediate in the transport of SV 40 to the ER ( L . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptors ( ER alpha and ER beta ) , NOS , heat shock proteins 70 and 90 ( HSP 70 and HSP 90 ) , caveolin 1 , 2 , and 3 , calmodulin , NOS activity , and cGMP were analyzed in a lysate of platelets from gonadally intact and ovariectomized female pigs . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| At the level of the ER , caveolin 2 interacts with caveolin 1 to form a high molecular mass hetero oligomeric complex that is targeted to lipid rafts and drives the formation of caveolae . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 is a recently identified co activator of ER alpha mediating the ligand independent activation of this steroid receptor . ^^^ We found that ectopic expression of ER alpha in SK N MC cells ( SK ER alpha ) leads to a ligand independent transcriptional suppression of caveolin 1 / 2 genes . ^^^ The analysis of selected promoter regions of the human caveolin 1 gene showed that certain CpG dinucleotides were hypermethylated in SK ER alpha cells , whereas the same sites were unmethylated in control , ER beta , and ER alpha / beta co expressing SK N MC cells . ^^^ Inhibition of DNA methylation or histone deacetylation led to partial re expression of caveolin 1 / 2 genes in SK ER alpha . ^^^ In conclusion , we have shown for the first time that ER alpha and not ER beta silences caveolin 1 / 2 expression in an epigenetic fashion in neuronal cells . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| The S522A mutant was also 60 % less effective than wt ERalpha in binding caveolin 1 , which facilitates ER transport to the PM . ^^^ Upon transfection in CHO cells , the S522A mutant ERalpha resulted in a 62 % decrease in membrane receptor number and reduced colocalization with caveolin 1 relative to those with expression of wt ERalpha . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| A recently identified co activator of ERalpha is caveolin 1 which has been shown to mediate the ligand independent activation of this steroid receptor . ^^^ In the present study we have demonstrated that neuronal SK N MC cells lacking functional ERalpha show high levels of caveolin 1 / 2 specific transcripts and proteins . ^^^ Ectopic expression of ERalpha in SK N MC cells leads to the transcriptional suppression of caveolin 1 and 2 genes . ^^^ Certain CpG dinucleotides were methylated in the caveolin 1 promoter region of the SK ERalpha cells whereas the same sites were non methylated in control SK N MC cells , implicating a gene silencing mechanism including hypermethylation of DNA . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptors ( ERalpha and ERbeta ) , heat shock proteins ( hsp 70 and hsp 90 ) , endothelial nitric oxide synthases ( eNOS ) , caveolin 1 , 2 and 3 and calmodulin were analyzed in total platelet lysate by immunoblotting . ^^^ Expression of ERalpha and ERbeta , hsp 70 , hsp 90 , eNOS , calmodulin , and caveolin 1 were observed in both sexes . ^^^ Human platelets contain estrogen receptor alpha , caveolin 1 and estrogen receptor associated proteins . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| We used the fact that wild type caveolin 1 accumulates in LDs after brefeldin A treatment or when linked to an ER retrieval motif to search for mutants defective in LD targeting . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| The methylation status of caveolin 1 gene had no clear relationship with age , cell grade , stage of tumor , and status of estrogen receptor , p 53 and c erbB 2 in the breast cancer tissues . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| The effect is mediated by estrogen receptors ( ERs ) , consistent with colocalization of ERalpha and caveolin 1 immunoreactivity at the plasma membrane of endothelial cells lining cerebral arteries . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Fluorescence immunolabeling analysis showed that ER alpha is present in both nuclei and plasma membranes in UAECs , and the latter is also colocalized with caveolin 1 . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Western analysis demonstrated that mERhigh cells expressed caveolin 1 and caveolin 2 , and that ER alpha was contained in the same gradient separated membrane fractions . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Here , we further demonstrated that ER alpha but not ER beta expression was constitutively activated in these caveolin 1 haploinsufficient cells . ^^^ Transient treatment of MCF10A cells with beta methyl cyclodextrin , a chemical that can displace caveolin 1 from the plasma membrane , also stimulated ER alpha expression . ^^^ These results suggest that dysregulation of caveolin 1 is one of the mechanisms by which ER alpha expression is activated during initiation of breast tumorigenesis . . ^^^ Caveolin 1 down regulation activates estrogen receptor alpha expression and leads to 17beta estradiol stimulated mammary tumorigenesis . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| We recently found the translocation of ERalpha to the membrane in the absence of estrogen is dependent on caveolin 1 and serine 522 of the ERalpha protein . ^^^ Similarly , deletion of the caveolin 1 scaffolding domain ( amino acids 60 100 ) largely prevents the localization of ERalpha at the plasma membrane . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| In addition to providing further evidence that ERalpha interacts with caveolin / caveolae in uterine smooth muscle cells , this study also shows that the interaction between caveolin 1 and ERalpha is actually facilitated by E 2 . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| In the present study , we investigated the expression of the two estrogen receptors , ERalpha and ERbeta , and their influence on regulation of the glucose transporter , GLUT 4 , and its associated structural protein , caveolin 1 , in mouse gastrocnemius muscle . ^^^ By immunofluorescence and Western blotting , caveolin 1 expression was higher in ArKO mice and lower in ERbeta ( / ) and ERalpha ( / ) mice than in WT littermates . ^^^ GLUT 4 and caveolin 1 were colocalized in WT and ArKO mice but not in ERbeta ( / ) and ERalpha ( / ) mice . ^^^ Both ERbeta and ERalpha are necessary for optimal caveolin 1 expression . ^^^ Taken together , these results indicate that colocalization of caveolin 1 and GLUT 4 is not an absolute requirement for muscle glucose metabolism but that reduction in GLUT 4 could be contributing to the insulin resistance observed in ERalpha ( / ) mice . . ^^^ |
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| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| We now show colocalization of NSP 4 and caveolin 1 in NSP 4 transfected and rotavirus infected mammalian cells in reticular structures surrounding the nucleus ( likely ER ) , in the cytosol , and at the cell periphery by laser scanning confocal microscopy . ^^^ We propose that the association of NSP 4 and caveolin 1 contributes to NSP 4 intracellular trafficking from the ER to the cell surface and speculate that exogenously added NSP 4 stimulates signaling molecules located in caveola microdomains . . ^^^ |
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| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Increased ER signaling in response to lapatinib is enhanced by the activation of factors facilitating the transcriptional activity of ER , notably FOXO3a and caveolin 1 . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Adsorption of Gd3+ and Mn2+ into hydroxyapatite ( HAP ( Ca 5 ( PO 4 ) 3 ( OH ) 2 ) was studied by ESR , ion chromatography and ICP AES to investigate the U uptake model . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptor and other members of the nuclear receptor superfamily may provide a mechanism to regulate CAV activity in situations of low virus copy number . . ^^^ |
|
| Interacting proteins: Q03135 and P03372 |
Pubmed |
SVM Score :0.0 |
| Our results revealed the existence of a virus activated endocytic pathway from the plasma membrane to the ER that involves neither clathrin nor caveolae and that can be activated also in the presence of cav 1 . . ^^^ |
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