| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.61389032 |
| The fusion protein ( FP ) produced by one clone immunoadsorbed autoantibodies , which specifically recognized the BP 180 by antigen , showing no cross reactivity with BP 240 by immunoblot analysis . 0.61389032^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| In BP , the antigens involved in the autoimmunity are epidermal basement membrane peptides BPAg 1 and BPAg 2 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Bands corresponding to desmoglein 3 , desmoglein 1 , BPAg 2 , BPAg 1 , and type 7 collagen were found in 38 sera . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| A number of immunoblot analyses have indicated that two major antigenic proteins of epidermal extracts , the 230 kDa BP antigen ( BP 230 or BPAG 1 ) and the 180 kDa BP antigen ( BP 180 , BPAG 2 or type 17 collagen ) , are detected by sera from patients with BP in various patterns . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Of the constituents of hemidesmosomes , the plaque protein , BP 230 ( BPAG 1 ) , and the collagen like transmembrane protein , BP 180 ( BPAG 2 ) , are the autoantigens of bullous pemphigoid and pemphigoid gestationis , the manifestations of both of which include subepidermal blistering . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| These autoantibodies show a linear staining at the dermal epidermal junction ( DEJ ) and recognize two major hemidesmosomal proteins , the BP 230 ( BPAG 1 ) and BP 180 ( BPAG 2 ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| These autoantibodies show a linear staining at the dermal epidermal junction , activate complement , and recognize two major hemidesmosomal antigens , BP 230 ( BPAG 1 ) and BP 180 ( BPAG 2 or type 17 collagen ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The antigen specificity of the patient ' s circulating antibodies was determined by Western blotting and enzyme linked immunoabsorbent assay ( ELISA ) using various antigen sources , including cultured human keratinocytes , dermal protein lysates , and purified laminin 5 , as well as proteins corresponding to BP 180 , the 230 kDa bullous pemphigoid antigen ( BP 230 ) , laminin 5 subunits , and collagen 4 alpha 1 alpha6 chains . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| In the skin , laminins are part of a hemidesmosome complex essential for basal keratinocyte adhesion that includes collagen 17 ( BP 180 ) and BPAG 1 ( dystonin / BP230 ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Transforming growth factor beta up regulates the expression of the genes for beta 4 integrin and bullous pemphigoid antigens ( BPAG 1 and BPAG 2 ) in normal and transformed human keratinocytes . ^^^ Three distinct proteins , namely , beta 4 integrins , and the 230 kDa ( BPAG 1 ) and 180 kDa ( BPAG 2 ) bullous and pemphigoid antigens , have been shown to co localize with hemidesmosomes at the dermal epidermal basement membrane zone . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The BPAG 2 gene was mapped by chromosomal in situ hybridization to the long arm of human chromosome 10 , at locus 10q24 . 3 ; this location is distinct from any previously mapped collagens , and it also distinguishes BPAG 2 from BPAG 1 , a 230 kDa bullous pemphigoid antigen , which has been mapped to chromosome 6p ( Sawamura , D . , Nomura , K . , Sugita , Y . , Mattei , M . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Bullous pemphigoid is an acquired blistering skin disease associated with the production of IgG autoantibodies to 230 kDa BP Ag ( BPAg 1 ) and to 180 kDa BP Ag ( BPAg 2 ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Human autoantibodies against the 230 kD bullous pemphigoid antigen ( BPAG 1 ) bind only to the intracellular domain of the hemidesmosome , whereas those against the 180 kD bullous pemphigoid antigen ( BPAG 2 ) bind along the plasma membrane of the hemidesmosome in normal human and swine skin . ^^^ Bullous pemphigoid ( BP ) is a blistering skin disease in which autoantibodies develop to hemidesmosomal components of the epidermal basement membrane zone , including two major antigenic proteins of the 230 kD antigen ( BPAG 1 ) and the 180 kD antigen ( BPAG 2 ) . ^^^ The present study demonstrated the precise ultrastructural localization of the epitopes for autoantibodies against BPAG 1 and BPAG 2 in normal skin . ^^^ Autoantibodies against either BPAG 1 or BPAG 2 were affinity purified using nitrocellulose membrane , which was blotted with SDS PAGE fractionated antigens from human epidermal extract as the immunoabsorbent . ^^^ These results suggest that the autoantibodies against BPAG 1 and BPAG 2 react with the epitopes localizing in distinct regions of the hemidesmosome complex , and may play different roles in the blister formation in patients with BP . . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| These autoantibodies react with antigens of 180 kDa and 230 kDa relative molecular mass , which are presumably identical to the bullous pemphigoid antigen BPAG 1 and BPAG 2 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The purpose of this study is to clarify a relationship between bullous pemphigoid autoantibodies against two major BP antigens , 230 kilodalton ( kD ) ( BPAG 1 ) and 180 kD ( BPAG 2 ) and their binding sites , using immunoblot analysis and indirect immunofluorescence on salt split skin . ^^^ Of 42 recognizing both BPAG 1 and BPAG 2 , 35 showed the epidermal pattern and seven showed the combined pattern . ^^^ Our results suggest that autoantibodies that react solely with BPAG 1 bind exclusively to the epidermal side of salt split skin and never show either a combined or a dermal pattern , and that most antibodies against BPAG 2 bind to the epidermal side . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| BPAG 1 and BPAG 2 : identification of a new region of homology between mouse and human chromosomes . ^^^ Two bullous pemphigoid antigens , BPAG 1 and BPAG 2 , have been recently cloned and mapped to human chromosomes 6p12 p 11 and 10q24 . 3 , respectively . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Immunoaffinity purified anti M 168 antibodies did not bind to the 230 kDa bullous pemphigoid antigen ( BPAg 1 ) or to the 180 kDa BPAg 2 . ^^^ This study demonstrates the specificity of some cicatricial pemphigoid sera against a 168 kDa antigen that is different from the laminin 5 subunits and shares no epitopes with the antigens of bullous pemphigoid ( BPAg 1 , BPAg 2 ) or the epidermolysis bullosa acquisita . . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| One hundred and eighty two of the 263 sera ( 69 % ) reacted by IB with BP antigens ( Ag ) , most commonly the BPAg 1 ( 93 cases , 51 % ) , and a complex of BPAg 1 and the 180 kDa minor BP antigen ( BPAg 2 ) ( 47 cases , 26 % ) . ^^^ A good correlation was found between the detection of autoantibodies by IIF and labelling of BPAg 1 and / or BPAg 2 by IB assay , in which 152 of 198 sera with an epidermal pattern in IIF identified a BP antigen . ^^^ Thirty one percent ( 13 of 42 ) of sera recognizing by IB BPAg 2 , were negative by IIF , as compared with 12 % ( 11 of 93 ) of those recognizing BPAg 1 ( P < 0 . 01 ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Sera from patients with bullous pemphigoid ( BP ) from the United States ( US ) , Japan , and Britain demonstrate similar reactivity to the major target antigens BPAG 1 and BPAG 2 . ^^^ Sera from patients and controls with BP from the US and Japan were assayed for reactivity to intact BPAG 1 and BPAG 2 by immunoblot , and to fusion proteins encoded by BPAG 1 by immunoblot and enzyme linked immunosorbant assay ( ELISA ) . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Pemphigoid gestationis and bullous pemphigoid are autoimmune diseases characterized by subepidermal blisters and antibodies against the hemidesmosomal antigens : BPAG 1 and BPAG 2 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Hemidesmosomes contain the plaque protein BPAG 1 and the partly collagen like transmembrane protein BPAG 2 , representing the autoantigens of bullous pemphigoid and pemphigoid gestationis with subepidermal blistering . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Hemidesmosomes contain the plaque protein BPAG 1 and the partly collagen like transmembrane protein BPAG 2 , representing the autoantigens of bullous pemphigoid and pemphigoid gestations which show subepidermal blistering . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Indirect immunofluorescence revealed normal linear labeling with antibodies against hemidesmosomal components ( alpha 6 and beta 4 integrins , BPAG 1 , and BPAG 2 ) and against anchoring filament components such as uncein , as detected by the 19 DEJ 1 monoclonal antibody . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| RNA was isolated from papulonodular basal cell carcinoma , normal human epidermal keratinocytes , and normal human skin , reverse transcribed to cDNA and amplified by the polymerase chain reaction utilizing primers specific for the 230 kDa bullous pemphigoid antigen ( BPAG 1 ) , the 180 kDa bullous pemphigoid antigen ( BPAG 2 ) , the alpha 6 and beta 4 chains of the alpha6beta4 integrin complex , and the beta 3 chain of laminin 5 . ^^^ The mean polymerase chain reaction product intensities were significantly less in the basal cell carcinoma as compared with the normal human epidermal keratinocytes at the following levels : p < 0 . 001 for alpha 6 and beta 4 integrins and the beta 3 chain of laminin 5 ; p < 0 . 01 for BPAG 1 ; and p < 0 . 05 for BPAG 2 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The fates of BPAG 2 , 230 kDa BPA ( BPAG 1 ) and bound BP IgG were traced by immunofluorescence microscopy using monoclonal antibodies to BPAG 1 , BPAG 2 and human IgG . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Using postembedding immunogold electron microscopy , we previously demonstrated that autoantibodies against the 230 kDa BP antigen ( BPAG 1 ) bind to the intracellular hemidesmosomal component of normal skin , whereas those against the 180 kDa BP antigen ( BPAG 2 ) bind only along the plasma membrane of hemidesmosomes . ^^^ Samples of fresh perilesional skin were obtained from three patients with BP whose sera reacted only with BPAG 1 , only with BPAG 2 , and with both BPAG 1 and BPAG 2 upon immunoblotting using epidermal extracts . ^^^ This distribution corresponded to the location of BPAG 2 , but not to that of BPAG 1 . ^^^ These findings suggest that most , if not all , of the in vivo deposited IgG antibodies in the perilesional skin of BP are directed against BPAG 2 , rather than against BPAG 1 , thus further supporting the crucial role of anti BPAG 2 autoantibody in the initial stage of blister formation in BP . . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| BP , CP and pemphigoid gestationis are characterized by the production of autoantibodies mainly directed against two hemidesmosomal proteins : BPAG 1 and BPAG 2 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of the HD associated structural proteins , bullous pemphigoid antigens 1 ( BPAG 1 ) and 2 ( BPAG 2 ) , was demonstrated in NHBE cells by immunofluorescence microscopy and immunoblot analyses . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| This observation suggests the presence of hitherto uncharacterized 200 and 280 kDa hemidesmosomal proteins distinct from BPAG 1 , BPAG 2 and beta 4 integrin as target antigens in linear IgA disease . . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| As the BP autoantigen primary structures are known , the question was addressed as to whether it is possible to demonstrate circulating antibodies against BP autoantigens ( BPAG 1 and BPAG 2 ) by means of an ELISA system , using antigenic epitopes . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Bands corresponding to desmoglein 1 and 3 , desmoplakin 1 and 2 , periplakin , BPAG 1 , BPAG 2 and type 7 collagen were identified distinctly with these sera . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Bullous pemphigoid ( BP ) is an autoimmune blistering skin disease characterized by the presence of autoantibodies to normal components of the hemidesmosome ( BPAg 1 and BPAg 2 ) . ^^^ IgG autoantibodies to BPAg 1 and BPAg 2 were detected in both blister fluid and serum of patients , whereas IgE autoantibodies , in much lower titers , were detected to only BPAg 1 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The serum level of autoantibodies against autoantigens of the bullous pemphigoid peptides 1 and 2 ( BPAG 1 and BPAG 2 ) is a relevant diagnostic marker . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Using bovine gingiva lysate as substrate in an immunoblot assay , autoantibodies to human bullous pemphigoid antigens ( BPAg 1 and BPAg 2 ) , human beta 4 integrin , and laminin 5 were measured . ^^^ The titers of antibodies to BPAg 1 and BPAg 2 did not correlate with disease activity or response to therapy . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| The 230 kD bullous pemphigoid antigen ( BPAG 1 ) gene expression was also reduced by RA , whereas transcript levels of integrin alpha 6 , integrin beta 4 , the 180 kD bullous pemphigoid antigen ( BPAG 2 ) , and laminin 5 were not affected . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| By immunoblotting , IgG of 13 sera ( 46 % ) reacted with the 180 kD bullous pemphigoid antigen ( BPAg 2 ) , six ( 21 % ) reacted with the 230 kD bullous pemphigoid antigen ( BPAg 1 ) , and two ( 7 % ) reacted with an antigen of 200 kD . ^^^ IgA of three sera ( 11 % ) reacted with BPAg 2 and three ( 11 % ) reacted with BPAg 1 . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| Lesions develop when autoantibodies are directed against glycoproteins BPAG 1 and BPAG 2 , hemidesmosomes that are embedded in the epidermal basement membrane . ^^^ |
|
| Interacting proteins: Q9UMD9 and Q03001 |
Pubmed |
SVM Score :0.0 |
| It is mediated by circulating antibodies directed against two hemidesmosomal proteins of the dermal epidermal junction : BPAG 1 and BPAG 2 . ^^^ |
|