| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Transcription factors in renal development : the WT 1 and Pax 2 story . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| In the mesenchyme , the early induction response requires at least two transcription factors , WT 1 and Pax 2 . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Pax 2 and Pax 8 are known to enhance expression of wt 1 through this conserved regulatory element . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| A host of transcription factors , especially WT 1 and PAX 2 , play a significant role in modulating podocyte function . . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| This model is based on podocyte specific overexpression of Pax 2 and associated with a loss of Wt 1 expression . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| The Wilms ' tumor suppressor gene WT 1 is required for the kidney mesenchyme to respond to induction ; the transcription factor Pax 2 may regulate the aggregation and proliferation of the kidney mesenchyme immediately after induction . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Repression of Pax 2 by WT 1 during normal kidney development . ^^^ We have examined the spatial and temporal expression patterns of Pax 2 and the Wilm ' s tumor suppresser protein WT 1 with specific antibodies in developing mouse kidneys . ^^^ A marked increase in WT 1 protein levels coincided precisely with down regulation of the Pax 2 gene in the individual precursor cells of the visceral glomerular epithelium , suggesting a direct effect of the WT 1 repressor protein on Pax 2 regulatory elements . ^^^ To examine whether WT 1 could directly repress Pax 2 transcription , binding of WT 1 to three high affinity sites in the 5 ' untranslated Pax 2 leader sequence was demonstrated by DNAseI footprinting analysis . ^^^ Furthermore , co transfection assays using CAT reporter constructs under the control of Pax 2 regulatory sequences demonstrated WT 1 dependent transcriptional repression . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Comparative in situ hybridization analysis of PAX 2 , PAX 8 , and WT 1 gene transcription in human fetal kidney and Wilms ' tumors . ^^^ A zinc finger gene , WT 1 , which is involved in WT induction , encodes a DNA binding protein , and like PAX 2 and PAX 8 proteins is a transcription factor with an important role in kidney development . ^^^ We have compared the expression patterns of PAX 2 , PAX 8 , and WT 1 in fetal kidney and WTs by in situ hybridization . ^^^ The PAX 2 , PAX 8 , and WT 1 genes were transcribed in the condensed mesenchyme and early stages of epithelial differentiation in fetal kidney . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| RNA samples were electrophoretically separated in 1 . 2 % agarose gels , transferred to nylon membranes , and hybridized to random primer labeled PAX 2 , PAX 8 , and WT 1 probes . ^^^ PAX 2 and WT 1 expression were seen in all four primary WTs ; PAX 8 was seen in three of the four primary WTs . ^^^ Of the 12 heterotransplant Wilms ' tumors , PAX 2 , PAX 8 , and WT 1 were concomitantly expressed in seven tumors . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| These include the product of the Wilms ' tumor suppressor gene ( WT 1 ) , a mammalian paired homologue ( Pax 2 ) , and the N myc oncoprotein . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Like Wilms tumors , mRNA levels of WT 1 , IGF 2 , Pax 2 , and MK genes were higher than newborn kidney in the majority of the tumors . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Our data demonstrate that WT 1 , in addition to its known effects on insulin like growth factor 2 , platelet derived growth factor A , and Pax 2 transcription , is a powerful repressor of its own gene . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Unlike the classic triphasic Wilms ' tumor that contains blastema , stroma , and epithelial tubules , the anaplastic tumor expressed only marginal levels of insulin like growth factor 2 ( IGF 2 ) mRNA and imperceptible levels of the Wilms ' tumor gene ( WT 1 ) , Pax 2 , and Pax 8 mRNA . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| The paired box transcription factor , PAX 2 , positively modulates expression of the Wilms ' tumor suppressor gene ( WT 1 ) . ^^^ In this report , we demonstrate that PAX 2 isoforms are capable of transactivating the wt 1 promoter . ^^^ Deletion mutagenesis of the wt 1 promoter identified an element responsible for mediating PAX 2 responsiveness , located between nucleotides 33 and 71 relative to the first wt 1 transcription start site . ^^^ Finally , we demonstrate that PAX 2 can stimulate expression of the endogenous wt 1 gene . ^^^ These results suggest that a role for PAX 2 during mesenchyme to epithelium transition in renal development is to induce wt 1 expression . . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Recent , data suggest that the Wilms ' tumor suppressor gene WT 1 can down regulate Pax 2 expression , consistent with high levels of Pax 2 in Wilms ' tumors . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Pax 2 and c ret expressions in the Wolffian duct and WT 1 and GDNF expressions in the metanephric blastema were initially normal in the mutant . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Differential regulation of the human Wilms tumour suppressor gene ( WT 1 ) promoter by two isoforms of PAX 2 . ^^^ PAX 2 and WT 1 encode transcription factors expressed during fetal kidney development in patterns that overlap both spatially and temporally . ^^^ The overlap of PAX 2 and WT 1 expression in fetal kidney prompted us to determine whether PAX 2 regulates the WT 1 gene . ^^^ To investigate this possibility , the WT 1 promoter and a series of WT 1 promoter deletion fragments were cloned into a luciferase reporter vector , and used in co transfection experiments with PAX 2 expression constructs . ^^^ PAX 2 transactivated the WT 1 promoter up to 35 fold in CHO K 1 cells , and from four to sevenfold in 293 cells . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| A more detailed function in kidney development has been postulated for transcription factors such as WT 1 , Pax 2 or other molecules such as glial cell line derived neurotrophic factor ( GDNF ) , Wnt 4 , c ret . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| The Wilm ' s tumor suppressor gene , WT 1 , and Pax 2 regulate the mesenchymal epithelial transition that occurs during nephrogenesis and both these genes exhibit altered expression patterns and / or are mutated in renal tumors . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| There are now 11 genes that satisfy these conditions and thus have been shown to be crucial for metanephric kidney development : WT 1 , Pax 2 , c ret , GDNF , alpha8beta1 , Wnt 4 , BF 2 , BMP 7 , PDGF B , PDGFRbeta , and alpha3beta1 . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| The WT 1 tumor suppressor protein , which has previously been shown to repress murine Pax 2 expression in vitro , was shown to also repress expression from the human PAX 2 promoter . . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| WT 1 and PAX 2 podocyte expression in Denys Drash syndrome and isolated diffuse mesangial sclerosis . ^^^ One target gene of WT 1 is PAX 2 , the expression of which is down regulated in podocytes during early stages of nephrogenesis . ^^^ We demonstrate that WT 1 mislocalization is associated with abnormal podocyte expression of PAX 2 protein and RNA . ^^^ We suggest that persistent expression of PAX 2 is likely to result from the loss of WT 1 dependent transcriptional repression and may participate in the pathological mechanisms leading to glomerular dysfunction . ^^^ Abnormal distribution of WT 1 and PAX 2 was also observed in isolated diffuse mesangial sclerosis suggesting that a defect in WT 1 could also be operative in isolated diffuse mesangial sclerosis . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Data from gene ablation studies in mice have indicated critical roles for Lim 1 , Wnt 4 , WT 1 , and Pax 2 in the coordination and execution of kidney patterning and differentiation . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that several markers , including Pax 2 , Six 2 , and GDNF , were present as RNAs in the metanephric mesenchyme of Wt 1 / embryos . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Examples of these genes include glial cell line derived neurotrophic factor ( GDNF ) , RET , PAX 2 , Wilms tumor suppressor ( WT 1 ) , and components in the renin angiotensin pathway . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| In addition , elevated Notch signaling in the pronephric anlage both perturbed the characteristic pattern of the differentiated tubule network and increased the expression of early markers of pronephric precursor cells , Pax 2 and Wilms ' tumor suppressor gene ( Wt 1 ) . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| We further provide evidence that vhnf 1 regulates the expression of key patterning genes for these organs . vhnf 1 is required for the proper expression of pdx 1 and shh ( sonic hedgehog ) in the gut endoderm , pax 2 and wt 1 in the pronephric primordial , and valentino ( val ) in the hindbrain . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Using RT PCR , a significant reduction in WT 1 mRNA expression was detected in the PAX 2 mutant glands compared to wild type counterparts and double antibody immunohistochemistry detected the co localization of PAX 2 and WT 1 in the nuclei of normal and cancerous breast cells . ^^^ These data indicate a role for PAX 2 ( and possibly WT 1 ) in the regulation of the progesterone response of the mature mammary gland . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| WT 1 and Pax 2 are transcription factors involved in kidney development and phenotypic regulation of glomerular epithelial cells . ^^^ However , the role of WT 1 and Pax 2 in the development of CLs in primary FSGS is unclear . ^^^ Using immunohistochemistry , the expression of WT 1 , Pax 2 , and cytokeratin ( CK ) , an epithelial marker never found in normal podocytes , was examined in 35 biopsy samples of primary FSGS . ^^^ Expression of WT 1 , Pax 2 , and CK was scantly positive in CSs . ^^^ WT 1 expression was decreased in CLs compared with unaffected podocytes , but Pax 2 and CK were strongly expressed in CLs and podocytes of morphologically unaffected tufts in cases with CLs . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| As nephrons form , they express critical transcription factors such as WT 1 , Pax 2 , and Hoxa 11 and d 11 , condense , and secrete Wnt 4 . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| These transcription factors include Pax 2 , WT 1 ( the Wilms tumor suppressor gene ) , Pod 1 ( capsulin , epicardin ) , Kreisler ( maf 1 ) , lmx1b , and mf 2 . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| In these triple mutants , the metanephric blastema condenses , and expression of early patterning genes , Pax 2 and Wt 1 , is unperturbed . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| METHODS : Using immunohistochemical techniques , we studied the podocyte expression of the transcription factor WT 1 and its target PAX 2 , GLEPP 1 , synaptopodin and vimentin as markers of podocyte maturity and of proliferating cell nuclear antigen ( PCNA ) as a marker of proliferation . ^^^ RESULTS : Abnormal distribution of WT 1 and PAX 2 and extensive loss of podocyte markers were observed in ICG and HIV AN ; this dysregulation was associated with podocyte proliferation without detectable apoptosis . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| These cells coexpressed adult epithelial ( keratin ) and embryonic mesenchymal ( vimentin , osteopontin , FSP 1 , PAX 2 , and WT 1 ) genes . ^^^ WT12S immortalized cells grown on or in Matrigel formed cysts or tubules , consistent with their expression profiles , which consisted of both epithelial and adult kidney markers ( E cadherin , alpha catenin , circumferential actin filaments ( CAF ) , alkaline phosphatase , aminopeptidase M , BMP 7 , or podocalyxin ) , but not embryonic / mesenchymal markers ( PAX 2 or WT 1 ) . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| WT 1 and Pax 2 are transcription factors involved in kidney development and phenotypic regulation of glomerular epithelial cells . ^^^ To investigate the contribution of WT 1 and Pax 2 to the development of the CL in primary FSGS , immunohistological studies were performed using renal biopsy specimens on the expression of WT 1 , Pax 2 and cytokeratin ( CK ) , which is an epithelial marker but never found in normal podocytes . ^^^ The expression of WT 1 was decreased in the CL compared with unaffected podocytes , but Pax 2 and CK were expressed significantly in the CL and also in the cells morphologically recognized as podocytes in cases with CL . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Recently , abnormal expression of WT 1 and PAX 2 was shown in the podocytes in diffuse mesangial sclerosis ( DMS ) associated with DDS and isolated DMS . ^^^ Immunohistochemical analysis of WT 1 , PAX 2 , vimentin , cytokeratin , and epithelial membrane antigen was performed in the kidney specimens obtained at autopsy or surgery . ^^^ Abnormal expression of WT 1 and PAX 2 in the EHBCE was observed in both patients , supporting our hypothesis . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| In the present study , the fate of Wt 1 mutant cells in chimeric kidneys was assessed by in situ marker analysis , and immunocytochemistry was used to re examine the claim that glomerulosclerosis ( GS ) is caused by loss of WT 1 and persistent Pax 2 expression by podocytes . ^^^ The development of GS was preceded by widespread loss of ZO 1 signal in podocytes ( even in kidneys where < 5 % of glomeruli contained Wt 1 mutant podocytes ) , increased intra renal renin expression , and de novo podocyte TGF beta 1 expression , but not podocyte Pax 2 expression or loss of WT 1 , synaptopodin , alpha actinin 4 or nephrin expression . ^^^ However , podocytes in partially sclerotic glomeruli that still expressed WT 1 at high levels showed reduced vimentin expression , cell cycle re entry , and re expressed desmin , cytokeratin and Pax 2 . ^^^ The results suggest that : ( 1 ) GS is not due to loss of WT 1 expression by podocytes ; ( 2 ) podocyte Pax 2 expression reflects re expression rather than persistent expression , and is the consequence of GS ; ( 3 ) GS is mediated systemically and the mechanism involves activation of the renin angiotensin system ; and ( 4 ) podocytes undergo typical maturational changes but subsequently de differentiate and revert to an immature phenotype during disease progression . . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Possible regulation of Wilms ' tumour gene 1 ( WT 1 ) expression by the paired box genes PAX 2 and PAX 8 and by the haematopoietic transcription factor GATA 1 in human acute myeloid leukaemias . ^^^ PAX 2 , PAX 8 and GATA 1 are known physiological regulators of WT 1 . ^^^ In the present study , we analysed either bone marrow or blood samples of 43 AML patients for the expression levels of WT 1 , PAX 2 , PAX 8 and GATA 1 by real time reverse transcription polymerase chain reaction ( LightCycler ) . ^^^ PAX 2 expression was found in 11 of 43 AML samples , with a clear correlation of PAX 2 with WT 1 expression levels observed . ^^^ In conclusion , PAX 2 , and possibly PAX 8 , appears to be a candidate for the upregulation of WT 1 in a proportion of AML , whereas GATA 1 expression can not be explained as an inducer of WT 1 . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| WT 1 is a modifier of the Pax 2 mutant phenotype : cooperation and interaction between WT 1 and Pax 2 . ^^^ Metanephric kidney development requires an inductive interaction between the ureteric bud and progenitor mesenchyme , where the early expression of two genes , Wilms ' tumour 1 ( WT 1 ) and paired box 2 ( Pax 2 ) , establishes critical but unknown developmental pathways . ^^^ Indeed , transgenic mice with deregulated overexpression of Pax 2 exhibit structural kidney defects and impaired renal function , as do mice harboring targeted disruptions and / or spontaneous mutations of either the Pax 2 or WT 1 genes . ^^^ WT 1 and Pax 2 are thought to regulate each other ' s expression during renal development . ^^^ To better define the relationship between WT 1 and Pax 2 , we generated mouse embryos containing heterozygous mutations in both genes . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| In line with established hypotheses about genetic pathways that control kidney development , we find that repressing Pax 2 using siRNAs represses Wt 1 expression and blocks both bud growth and nephron differentiation , but that repressing Wnt 4 blocks nephron differentiation without affecting Wt 1 expression . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Many of the genes that play a crucial role in early kidney development , such as Pax 2 , Eya 1 , Six 1 , Six 2 , Sall 1 , Foxc 1 , Wt 1 , and the Hox 11 genes , are expressed in the mesenchyme and encode transcription factors that with few exceptions are involved in regulation of the Gdnf gene . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis detected gene expression of Pax 2 , Lim 1 , c Ret , Emx 2 , Sall 1 , WT 1 , Eya 1 , GDNF , and Wnt 4 in the EB outgrowths from days 6 9 of expansion onward , and also in the teratoma tissues 14 and 28 days after transplantation . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| Detailed molecular marker analyses show that key regulators of early intermediate mesoderm development , including Lhx 1 , Pax 2 , and Wt 1 , are all down regulated and nephrogenic mesenchyme undergoes massive apoptosis , resulting in disruption of nephric duct elongation and failure of metanephric induction in the Odd 1 ( / ) mutant embryos . ^^^ |
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| Interacting proteins: Q02962 and P19544 |
Pubmed |
SVM Score :0.0 |
| PAX 2 activation caused repression of the podocyte key regulator molecule Wt 1 and consequently a dramatic reduction of nephrin expression . ^^^ Recruitment of the groucho related protein TLE 4 may be involved in converting Pax 2 into a transcriptional repressor of Wt 1 . ^^^ |
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