| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.54080001 |
| In this study we demonstrate that endogenous MEKK 1 binds to endogenous ERK 2 , MEK 1 , and another MEKK level kinase , Raf 1 , suggesting that it can assemble all three proteins of the ERK 2 MAP kinase module . . 0.54080001^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| MEKK 1 phosphorylates MEK 1 and MEK 2 but does not cause activation of mitogen activated protein kinase . ^^^ A constitutively active fragment of rat MEK kinase 1 ( MEKK 1 ) consisting of only its catalytic domain ( MEKK C ) expressed in bacteria quantitatively activates recombinant mitogen activated protein ( MAP ) kinase / extracellular signal regulated protein kinase ( ERK ) kinases 1 and 2 ( MEK 1 and MEK 2 ) in vitro . ^^^ However , in addition to its capacity to phosphorylate MEK 1 in vitro , MEKK C interacts with MEK 1 in the two hybrid system , and expression of mouse MEKK 1 or MEKK C in mammalian cells causes constitutive activation of both MEK 1 and MEK 2 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Raf and MAPK / extracellular signal regulated kinase kinase ( MEKK ) independently phosphorylate and activate MEK 1 . ^^^ Purified recombinant wild type MEK 1 and a mutant kinase inactive MEK 1 were used as substrates for MEKK , Raf , and autophosphorylation . ^^^ MEK 1 phosphorylation catalyzed by Raf , MEKK , or autophosphorylation resulted in activation of MEK 1 kinase activity measured by phosphorylation of a mutant kinase inactive MAPK . ^^^ MEK 1 is phosphorylated by MAPK at sites different from that for Raf and MEKK . ^^^ The results demonstrate that MEKK and Raf regulate MEK 1 activity by phosphorylation of common residues and thus , two independent protein kinases converge at MEK 1 to regulate the activity of MAPK . . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitated MEKK 2 phosphorylated the MAP kinase kinases , MEK 1 , and JNK kinase . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Receptor induced activation of other Ser / Thr kinases , such as c Raf , B Raf , Mek 1 , Mek 2 , Mekk , was not affected by wortmannin . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| These data suggest that the activation of the MAPK cascade in response to TNF alpha is mediated by the sequential activation of an MEKK and MEK 1 in a c Raf 1 and Raf B independent fashion . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Activation of MEKK was determined on immunoprecipitates from FMLP stimulated neutrophils by in vitro kinase assay , which utilized both MEK 1 and MEK 2 as substrates . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| In each kinase module , the upstream activator , a constitutively active mutant of MEK 1 or MEKK 1 , was expressed from a low copy plasmid , while one or two downstream effector kinases were expressed from a high copy plasmid with different antibiotic resistance genes and origins of replication . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Morphine enhanced the cellular levels of ERK 1 ( 44 kDa ) , ERK 2 ( 42 kDa ) , a 54 kDa ERK , MEK 1 ( 45 kDa ) , and MEKK ( 78 kDa ) . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Coexpression of dominant negative and constitutively active forms of CDC 42 , Rac 1 , Ras , MEKK 1 , and MEK 1 with JNK indicated that JNK activation by GnRH and TPA is mediated by CDC 42 and MEKK 1 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Protein kinase A ( PKA ) was found to enhance the activity by about eightfold , whereas both ERK ( extracellular signal regulated kinase ) activator kinase 1 ( MEK 1 ) and MEK kinase 1 ( MEKK 1 ) significantly repressed the activity . ^^^ Because RBCC proteins are presumed to act as a proto oncoprotein , these results suggest that the RBCK 1 protein is involved in the intracellular signaling cascades along with PKA , MEK 1 , and MEKK 1 and mediates cell growth and differentiation . . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Inhibition is selective for MEK 1 and 2 , as U 0126 shows little , if any , effect on the kinase activities of protein kinase C , Abl , Raf , MEKK , ERK , JNK , MKK 3 , MKK 4 / SEK , MKK 6 , Cdk 2 , or Cdk 4 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Addition of heptachlor to human CEM x 174 lymphocytic cells reduced the cellular levels of MAP kinase ( MAPK , mitogen activated protein kinase ) cascade proteins , including ERK 1 ( a 44 kDa MAPK ) , ERK 2 ( a 42 kDa MAPK ) , a 85 kDa and a 54 kDa MAP kinase , MEK 1 ( a 45 kDa ERK kinase ) and MEKK ( a 78 kDa MEK kinase ) . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Overexpression of upstream activators of MAP kinases , like MEKK 1 or MEK 1 , demonstrated that ADD1 / SREBP 1c mediated effects of insulin and PDGF might be linked to the MAP kinase cascade . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Co transfection of the hINV promoter with dominant negative forms of Ras , MEKK 1 , MEK 1 , MEK 7 , MEK 3 , p38 / RK , and c Jun inhibit the TPA dependent increase . ^^^ Wild type MEKK 1 enhances promoter activity and the activity can be inhibited by dominant negative MEKK 1 , MEK 1 , MEK 7 , MEK 3 , p38 / RK , and c Jun . ^^^ Additional pathways that transfer MEKK 1 activation via MEK 1 and MEK 7 also may function , but the downstream targets of these kinases need to be identified . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| A constitutively active form of MEKK 1 but not mitogen activated protein kinase kinase 1 ( MEK 1 ) or TGF beta activated kinase 1 , two distinct intracellular kinases , can specifically activate a Gal 4 Smad2 fusion protein , and this effect correlates with an increase in the phosphorylation state of the Smad 2 protein . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Although JSAP 1 coprecipitated with MEK 1 MAPKK and Raf 1 MAPKKK , and not MKK 6 or MKK 7 MAPKK , in cotransfected COS 7 cells , MEK 1 and Raf 1 do not interfere with the binding of SEK 1 and MEKK 1 to JSAP 1 , respectively . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Overexpression of upstream activators of the MAP kinases , like MEKK 1 or MEK 1 , stimulated LDL receptor promoter activity several fold in an sre 1 related manner . ^^^ Using the heterologous Gal 4 system , we could show that intracellular activation of the MAP kinase cascade by ectopic expression of MEKK 1 or MEK 1 has a direct stimulatory effect on the transcriptional activity of SREBP 1a and SREBP 2 . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion studies produced a marked reduction in immunoreactive Raf / MEKK content and activity , but a minimal decrease in the ability of chromatography fractions to phosphorylate and activate recombinant MEK 1 . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Although PD 98059 and dominant negative MEK 1 blocked Ang 2 induced activation of the ANF promoter , SB 203580 or dominant negative MEK kinase 1 ( MEKK 1 ) showed no effect . ^^^ Phenylephrine induced ANF promoter activation was significantly inhibited by SB 203580 and dominant negative MEKK 1 , but not by PD 98059 or dominant negative MEK 1 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Upregulation of MMP 1 expression by mitogenic or inflammatory stimuli is blocked by specific small molecular weight inhibitors of the ERK pathway or the p 38 pathway , respectively , and constitutively active kinases within the ERK1 / 2 pathway ( MEKK 1 , MEK 1 ) or the p 38 pathway ( ASK 1 , MEKK 1 , MKK 3 ) are potent activators of the MMP 1 promoter . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Intriguingly , the SMRT protein is a substrate for phosphorylation by protein kinases operating at multiple levels in this MAPKKK pathway , including the MAPKs , MAPK extracellular signal regulated kinase 1 ( MEK 1 ) , and MEK 1 kinase ( MEKK 1 ) . ^^^ Phosphorylation of SMRT by MEKK 1 and , to a lesser extent , MEK 1 inhibits the ability of SMRT to physically tether to its transcription factor partners . ^^^ Notably , activation of MEKK 1 or MEK 1 signaling in transfected cells also leads to a redistribution of the SMRT protein from a nuclear compartment to a more perinuclear or cytoplasmic compartment . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| ATP also inhibited the activation of cRaf 1 by other growth factors , epidermal growth factor and platelet derived growth factor , as well as other MEK 1 activators stimulated by FGF 2 , MEK kinase 1 ( MEKK 1 ) and MEKK 2 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Further analyses disclosed that transfection of dominant negative forms of raf 1 , MEK 1 , ERK 1 , ERK 2 , or wild type MEKK 1 all increased cystatin A promoter activity in normal human keratinocytes , whereas wild type raf 1 , ERK 1 , ERK 2 , or dominant negative forms of MEKK 1 , MKK 7 , or JNK 1 suppressed the promoter activity . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Jun N terminal kinase 1 activity , MEK 1 , 2 , MKK 4 , and MEKK 1 were constitutively activated in clones stably transfected to express Q226L Galpha 13 . ^^^ We demonstrate that the signal linkage map from Galpha 13 activation to primitive endoderm formation in these stem cells requires activation at three levels of the mitogen activated protein kinase cascade : MEKK 1 , 2 , or 4 for MAP kinase kinase kinase ; MKK 4 and / or MEK 1 for MAP kinase kinase ; and JNK 1 for MAP kinase . . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| We present data showing that overexpression of the serine threonine kinase , mitogen activated protein kinase / extracellular signal regulated kinase kinase kinase 1 ( MEKK 1 ) , but not nuclear factor kappa B inducing kinase , or MAP kinase / ERK kinase 1 ( MEK 1 ) , can significantly increase the level of CD28RE / AP1 driven luciferase ( Luc ) reporter gene expression in Jurkat E 6 1 cells . ^^^ Calcineurin inhibitors , CsA and FK 520 , or inhibitors of p 38 kinase ( SB 203580 ) , or MEK 1 ( PD 098059 ) , did not affect MEKK 1 induced reporter activation . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Co transfection of the MAO B promoter with dominant negative forms of Ras , Raf 1 , MEKK 1 , MEK 1 , MEK 3 , MEK 7 , ERK 2 , JNK 1 , and p38 / RK inhibit the PMA dependent activation of the MAO B promoter . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Therefore , our data suggest that H ( 2 ) O ( 2 ) regulates the activity of NF kappaB by MEK 1 activation through MEKK 1 dependent but Ras / Raf independent mechanism . . ^^^ |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| We identified the upstream kinase MEKK 1 as an activator of both the ERK1 / 2 and JNK 2 pathways , whereby the ERK1 / 2 kinase MEK 1 and the JNK kinase MKK 7 bind to MEKK 1 in a competing fashion . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| This cascade consists of Bck 1 ( a MEKK ) , the redundant Mkk 1 and Mkk 2 enzymes ( MEKs ) , and Mpk 1 ( a MAPK ) . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Although neither activation of BCK 1 ( MEKK ) by the dominant BCK 1 20 mutation nor increased dosage of MKK 1 ( MEK ) or MPK 1 ( MAP kinase ) mimicked PKC 1 as a glc 7 10 dosage suppressor , extra copies of genes encoding upstream components of the Pkc1p pathway such as ROM 2 , RHO 2 , HCS77 / WSC1 / SLG1 and MID 2 also suppressed glc 7 10 effectively . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Since MEKK 1 activates multiple parallel pathways , we examined involvement of JNK / SAPK , p 38 , and MKK 1 in promoter regulation . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Ubiquitylation of MEKK 1 inhibits its phosphorylation of MKK 1 and MKK 4 and activation of the ERK1 / 2 and JNK pathways . ^^^ MEKK 1 phosphorylates and activates MKK 1 and MKK 4 , leading to ERK1 / 2 and JNK activation . ^^^ The functional consequence of MEKK 1 ubiquitylation is the inhibition of MEKK 1 catalyzed phosphorylation of MKK 1 and MKK 4 resulting in inhibition of ERK1 / 2 and JNK activation . ^^^ The C441A mutation within the PHD of MEKK 1 prevents ubiquitylation and preserves the ability of MEKK 1 to catalyze MKK 1 and MKK 4 phosphorylation . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| Further , we show that growth factor induced uPA expression requires MEKK 1 dependent MKK 1 and JNK activity and that transfection of MEKK 1 into knockout cells restores inducible uPA expression and activity . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| MAP kinase kinase 3 ( MKK 3 ) was phosphorylated in response to LPS , and expression of constitutively active MKK 3 , but not the MAP kinase kinases MEKK 1 or MKK 1 , activated CYP2E1 . ^^^ |
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| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and Q13233 |
Pubmed |
SVM Score :0.0 |
| NA |
|