| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| PAK 1 phosphorylates MEK 1 on Ser 298 , a site important for binding of Raf 1 to MEK 1 in vivo . ^^^ Expression of interfering PAK 1 also reduces stimulation of TCF function by serum growth factors , while expression of active PAK 1 enhances EGF stimulated MEK 1 activity . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| Deletion of the binding site from MEK 1 reduced its phosphorylation by ERK 2 , but had no effect on its phosphorylation by p 21 activated protein kinase 1 ( PAK 1 ) . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| PAK 1 primes MEK 1 for phosphorylation by Raf 1 kinase during cross cascade activation of the ERK pathway . ^^^ Here we show that both Raf 1 and MEK 1 are phosphorylated by PAK 1 and that mutations at PAK 1 phosphorylation sites in either protein prevent cross cascade activation . ^^^ In contrast , MEK 1 activation by constitutively active Raf 1 is refractory to mutations at PAK 1 phosphorylation sites . ^^^ Our findings indicate that PAK 1 primes MEK 1 for activation by Raf 1 and imply another level of regulation in the ERK cascade . . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| However , upon replating of cells onto a fibronectin matrix , there was a substantial induction of MEK 1 ERK2 association and ERK activation , both of which could be inhibited by dominant negative PAK 1 . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| Here , we show that basic fibroblast growth factor ( bFGF ) and vascular endothelial growth factor ( VEGF ) differentially activate Raf , resulting in protection from distinct pathways of apoptosis in human endothelial cells and chick embryo vasculature . bFGF activated Raf 1 via p 21 activated protein kinase 1 ( PAK 1 ) phosphorylation of serines 338 and 339 , resulting in Raf 1 mitochondrial translocation and endothelial cell protection from the intrinsic pathway of apoptosis , independent of the mitogen activated protein kinase kinase 1 ( MEK 1 ) . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| PAK 1 phosphorylation of MEK 1 regulates fibronectin stimulated MAPK activation . ^^^ We find that adhesion to fibronectin induces PAK 1 dependent phosphorylation of MEK 1 on S 298 and that this phosphorylation is necessary for efficient activation of MEK 1 and subsequent MAPK activation . ^^^ We propose that FAK / Src dependent , PAK 1 mediated phosphorylation of MEK 1 on S 298 is central to the organization and localization of active Raf MEK 1 MAPK signaling complexes , and that formation of such complexes contributes to the adhesion dependence of growth factor signaling to MAPK . . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| The MEK 1 scaffolding protein MP 1 regulates cell spreading by integrating PAK 1 and Rho signals . ^^^ MP 1 associates with active but not inactive PAK 1 and controls PAK 1 phosphorylation of MEK 1 . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| Although Rac 1 deletion caused a significant reduction in phospho PAK 1 , AKT , and ERK under serum stimulation , reconstitution of active PAK 1 , but not AKT or MEK 1 , was able to rescue the actin cytoskeleton and adhesion phenotypes of the Rac 1 deficient cells . ^^^ Furthermore , Rac 1 deletion led to a marked increase in spontaneous apoptosis that could be rescued by active PAK 1 , AKT , or MEK 1 expression . ^^^ |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02750 and Q13153 |
Pubmed |
SVM Score :0.0 |
| NA |
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