Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.73715078
To do this , ERK 2 was quantitatively activated by MAPKK 1 in vitro by monitoring the stoichiometry and site specificity of phosphorylation using a combination of protein mass spectrometry , tryptic peptide analysis , and ( 32 ) P radiolabeling . 0.73715078^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.61705352
Cotransfection of dominant negative MEK 1 with a dominant negative mitogen activated protein kinase ( extracellular signal regulated protein kinase ( ERK 2 ) ) increased this inhibition . 0.61705352^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.6922387
Some mutations identified in this manner impaired the two hybrid interaction of ERK 2 with both MEK 1 and MEK 2 , whereas others had a predominant effect on the interaction with either MEK 1 or MEK 2 . 0.6922387^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.57386276
Consistent with its recognition of the MAP kinase insert , PEA 15 blocked activation of ERK 2 by MEK 1 , which also requires the MAP kinase insert to interact productively with ERK 2 . 0.57386276^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Since the only known targets of MAP kinase kinase 1 are Erk 1 and Erk 2 , these findings argue that MAP kinase function is required for the spindle assembly checkpoint in XTC cells . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To confirm a requirement for sustained ERK activation for megakaryocytic differentiation , PD 098059 , a synthetic inhibitor of the MAP kinase kinase 1 ( MEK 1 ) was employed . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment of cells with the MAP kinase kinase 1 ( MEK 1 ) inhibitor PD 98059 blocked IL 1beta induced activation of ERK 2 by more than 90 % but enhanced IL 1beta stimulated induction of PDGF Ralpha expression fourfold . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Nevertheless , activation of ERK by transient transfection of constitutively active mutant MAP kinase kinase 1 ( MKK 1 ) enhanced endogenous topoisomerase 2 activity by fourfold . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
UO 126 , an inhibitor of MAP kinase kinase 1 ( MEK 1 ) , suppressed focal adhesion targeting of active ERK and cell spreading . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
TGF beta ' s stimulation of ERK activation was blocked by PD 98059 , an inhibitor of MAP kinase kinase 1 , and by adenoviral transfer of dominant negative RasN 17 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
IFNbeta also induced phosphorylation of extracellular signal regulated kinase ( ERK ) mitogen activated protein kinase , and the MAP kinase kinase 1 ( MEK 1 ) inhibitor PD 98059 dose dependently inhibited beta chemokine mRNA and protein expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To evaluate a possible mechanism for the chronic regulation of MAPK / ERK kinase 1 ( MEK 1 ) and p 42 mitogen activated protein kinase ( MAPK ) we studied the long term effects of the G protein coupled receptor agonist endothelin 1 ( ET 1 ) and the protein tyrosine kinase coupled receptor agonist platelet derived growth factor BB ( PDGF BB ) on MEK 1 and p 42 MAPK in glomerular mesangial cells ( GMCs ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , the selective MAPK / ERK kinase 1 inhibitor PD 98059 ( 2 ' amino 3 ' methoxyflavone ) , and p 38 MAPK inhibitors SB 202190 ( 4 ( 4 fluorophenyl ) 2 ( 4 hydroxyphenyl ) 5 ( 4 pyridyl ) 1H imidazole ) and SB 203580 ( 4 ( 4 fluorophenyl ) 2 ( 4 methylsulfinylphenyl ) 5 ( 4 pyridyl ) 1H imidaz ole ) blocked A2B receptor mediated production of IL 8 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This morphogenetic behavior is profoundly subverted in MDCK C 7 cells expressing a constitutively active MAPK / ERK kinase 1 ( caMEK 1 ) mutant ( C 7 caMEK1 cells ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NRG 1 activated p42 / p44 mitogen activated protein kinase ( MAPK ) [ extracellular signal regulated kinase ( ERK ) 2 / ERK1 ] and ribosomal S 6 kinase ( RSK ) 2 ( 90 kDa ribosomal S 6 kinase ) , both of which could be inhibited by the MAPK / ERK kinase 1 antagonist PD 098059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 , which blocks the activation of MAPK / ERK kinase 1 ( MEK 1 ) , inhibited the increase in the activity of ERK 2 by infection of respiratory syncytial virus by about 50 % at 10 microM . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , the BDNF accelerated apoptosis was inhibited by the addition of SB 202190 and SB 203580 , specific inhibitors of p 38 mitogen activated protein kinase ( MAPK ) , and U 0126 , a specific inhibitor of MAPK / ERK kinase 1 , indicating that the activation of both p 38 MAPK and ERK is involved in the signaling cascade of the BDNF accelerated , NO donor induced apoptosis . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Lung fibroblasts were stimulated with BK in the presence or in the absence of PD 98059 , a specific MAPK / ERK kinase 1 inhibitor , or SB 203580 , a specific p 38 MAPK inhibitor , and IL 6 or IL 8 production and their gene expression was examined . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
An inhibitor of MAPK / ERK kinase 1 , PD 98059 , also inhibited ECGS stimulated DNA synthesis ( IC ( 50 ) , approximately 55 microM ) , but combining PD 98059 with MSeA had an effect similar to that when PD 98059 was used alone . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The dual phosphorylation of Thr 183 and Tyr 185 in ERK 2 is catalyzed by MAPK / ERK kinase 1 ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Dominant negative mutants of the upstream kinases MEK 1 ( MAPK / ERK kinase 1 ) , MKK ( MAPK kinase ) 6 and MKK 7 also inhibited IL 8 secretion , pointing to a role of all three MAPKs in HPE mediated IL 8 release . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Using a rat calvarial organ culture system , the inhibition of ERK phosphorylation by PD 98059 , a MAPK / ERK kinase 1 ( MEK 1 ) inhibitor , was assayed by immunoblotting . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activation of ERK1 / 2 by adenovirus mediated delivery of constitutively active MAPK / ERK kinase 1 resulted in the induction of MMP 19 expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Two highly conserved and hydrophobic protein binding sites are located on the opposite `` cytoplasmic ' ' face of the p14 / MP1 heterodimer and might therefore function as docking sites for the target proteins extracellular regulated kinase ( ERK ) 1 and MAPK / ERK kinase 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
GnRH induced phosphorylation of MAPK / ERK kinase 1 and c Raf kinase was blocked by Cam inhibition , whereas activity of phospholipase C was unaffected , suggesting that Ca2+ / Cam modulation of the ERK cascade potentially at the level of c Raf kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment with PD 98059 , which blocks the activation of MAPK / ERK kinase 1 , inhibited MPL induced IL 8 production by 64 . 4 % at 25 micromol / L . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The RG induced GDNF mRNA up regulation in C 6 glioblastoma cells was completely attenuated by the presence of a pan specific protein kinase C ( PKC ) inhibitor ( Ro 31 8220 ) and a MAPK / ERK kinase 1 ( MEK 1 ) inhibitor ( U 0126 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Constitutively active MAPKK 1 fully activates ERK 2 and the transcription factor Elk 1 , but does not substitute for activated p21ras and synergize with calcium / calcineurin signals to induce NFAT . ^^^ Thus , p21ras regulation of NFAT in T cells requires the activity of multiple effector pathways including those regulated by MAPKK 1 / ERK 2 and Rac 1 . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here we have investigated the mechanism through which a constitutively active form of human MAPKK 1 ( denoted MAPKK 1 R4F or MAPKK 1 * ) phosphorylates Xenopus p 42 MAPK in vitro . ^^^ These findings indicate that MAPKK 1 * phosphorylates p 42 MAPK by a two collision , distributive mechanism rather than a single collision , processive mechanism , and provide a mechanistic basis for understanding how MAP kinase can convert graded inputs into switch like outputs . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The structure of this protein kinase , denoted MEK 1 , for MAP kinase or ERK kinase , was elucidated from a complementary DNA sequence and shown to be a protein of 393 amino acids ( 43 , 500 daltons ) that is related most closely in size and sequence to the product encoded by the Schizosaccharomyces pombe byr 1 gene . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The prototype mitogen activated protein ( MAP ) kinase module is a three kinase cascade consisting of the MAP kinase , extracellular signal regulated protein kinase ( ERK ) 1 or ERK 2 , the MAP / ERK kinase ( MEK ) MEK 1 or MEK 2 , and the MEK kinase , Raf 1 or B Raf . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
It was possible , however , to activate Raf 1 , MEK 1 , and p42MAPK in J . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Following EGF stimulation of B82L cells expressing a kinase defective EGF receptor mutant ( K721M ) , we found that ERK 2 and ERK 1 MAP kinases , as well as MEK 1 and MEK 2 were all activated , and SHC became prominently tyrosine phosphorylated . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A constitutively active fragment of rat MEK kinase 1 ( MEKK 1 ) consisting of only its catalytic domain ( MEKK C ) expressed in bacteria quantitatively activates recombinant mitogen activated protein ( MAP ) kinase / extracellular signal regulated protein kinase ( ERK ) kinases 1 and 2 ( MEK 1 and MEK 2 ) in vitro . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Swiss 3T3 cells stably transfected with interfering mutants of MEK 1 showed a 60 % decrease in PDGF stimulated p 42 MAPK activation , but there was no inhibition of the mitogenic effect of forskolin in these cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inasmuch as hSOS is involved in p21ras activation , we also demonstrated that mIgM ligation activated a Ras dependent kinase cascade in which sequential activation of Raf 1 and MEK 1 culminates in the activation of p 42 mitogen activated protein ( MAP ) kinase ( ERK 2 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Stimulation of these cells with Ag , carbachol , Ca ( 2+ ) ionophore , or thapsigargin resulted in the phosphorylation of Raf 1 , MEK 1 , p42mapk MAP kinase , and the recently cloned cytosolic phospholipase A 2 ( PLA 2 ) and increased activities of both MAP kinase and PLA 2 , as well as release of arachidonic acid . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Regulation of the MAP kinase cascade in PC 12 cells : B Raf activates MEK 1 ( MAP kinase or ERK kinase ) and is inhibited by cAMP . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These data demonstrate that the activators of p 38 ( MKK 3 and MKK 4 ) , JNK ( MKK 4 ) , and ERK ( MEK 1 and MEK 2 ) define independent MAP kinase signal transduction pathways . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 activity , measured as autophosphorylation or as phosphorylation of a glutathione S transferase Erk fusion protein , was undetectable in unstimulated cells but became evident after treatment with chemoattractant . ^^^ The data suggest that MEK 1 is largely responsible for the activation of Erk in chemoattractant stimulated neutrophils and that protein kinase C and / or tyrosine kinases mediate this effect , whereas elevated cytosolic Ca2+ is not essential . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Previously , we have observed that overexpression of either ERK 1 , MEK 1 , or a constitutively active truncated form of c Raf 1 ( BXB ) is insufficient to activate AP 1 in REF 52 fibroblasts . ^^^ We therefore examined whether overexpression of small t either alone or in conjunction with ERK 1 , MEK 1 , or BXB could activate AP 1 . ^^^ We found that coexpression of small t and either ERK 1 , MEK 1 , or BXB resulted in an increase in AP 1 activity , whereas expression of either small t or any of the kinases alone did not have any effect . ^^^ Coexpression of kinase deficient mutants of ERK 1 and ERK 2 inhibited the activation of AP 1 caused by expression of small t and either MEK 1 or BXB . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We now demonstrate that three recombinant MEKs ( MEK 1 , MEK 2 , MEK 3 ) show remarkably different activity toward recombinant ERK 1 and ERK 2 . ^^^ MEK 3 , which is identical to MEK 1 except for missing an internal 26 amino acid residue and probably represents an alternative splicing product of MEK 1 , shows neither autophosphorylation nor ERK activating activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Interaction with SV 40 small tumor antigen ( small t ) compromised the ability of multimeric protein phosphatase 2A to inactivate the mitogen activated protein kinase ERK 1 and the mitogen activated protein kinase kinase MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 is a dual specificity kinase that phosphorylates and activates the Erk / MAP kinases Erk 1 and Erk 2 by phosphorylating them on threonine and tyrosine . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Both MEK 1 and MEK 2 were expressed in Escherichia coli and shown to be able to activate recombinant human ERK 1 in vitro . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A MAP kinase kinase ( MKK 1 or MEK 1 ) has been identified as a dual specificity protein kinase that is sufficient to phosphorylate MAP kinases p42mapk and p44mapk on the regulatory threonine and tyrosine residues . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Active Raf 1 phosphorylates and activates the mitogen activated protein ( MAP ) kinase / extracellular signal regulated kinase kinase 1 ( MEK 1 ) , which in turn phosphorylates and activates the MAP kinases / extracellular signal regulated kinases , ERK 1 and ERK 2 . ^^^ Therefore , we sought to determine whether MEK 1 and ERK activities also stimulate IL 2 gene transcription . ^^^ Expression of the MAP kinase specific phosphatase , MKP 1 , which blocks ERK activation , inhibited IL 2 promoter and NF AT driven transcription stimulated by a calcium ionophore and PMA , and in addition , MKP 1 neutralized the transcriptional enhancement caused by active Raf 1 and MEK 1 expression . ^^^ We conclude that the MAP kinase signal transduction pathway consisting of Raf 1 , MEK 1 , and ERK 1 and ERK 2 functions in the stimulation IL 2 gene transcription in activated T lymphocytes . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Characterization of ERK 1 activation site mutants and the effect on recognition by MEK 1 and MEK 2 . ^^^ To discern MEK 1 and MEK 2 specificity for their substrate , extracellular signal regulated kinase ( ERK ) , site directed mutagenesis was performed on the amino acid residues flanking the regulatory phosphorylation sites of ERK 1 . ^^^ These ERK 1 mutants were analyzed for the ability to act as a substrate for MEK 1 and MEK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
OSM also induced the activation of ERK 2 by activating the serine / threonine kinases Raf 1 and [ mitogenactivated protein kinase ( MAPK ) ERK kinase ( MEK 1 ) ] 1 , while bFGF failed to activate any of the above components . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
FBS , ET 1 , and PDGF BB led to a time dependent increase in MEK 1 mRNA and protein expression without altering p 42 MAPK mRNA and protein levels . ^^^ In GMC , cycloheximide inhibited MEK 1 mRNA induction but stimulated p 42 MAPK mRNA expression in the absence and presence of FBS , ET 1 , or PDGF . ^^^ The FBS induced increase in MEK 1 mRNA was accompanied by a sustained enhancement of MEK activity , as assessed by the ability of immunoprecipitated p 45 MEK to activate recombinant p 42 MAPK and hence phosphorylate myelin basic protein , and p 42 MAPK activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : In RAW 264 . 7 mouse macrophages , both ERK 2 and MEK 1 activity are induced by LPS treatment . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , phosphorylation of Raf 1 , MEK 1 , p42mapk , and cytosolic phospholipase A 2 , as well as the associated increase in MAP kinase activity and release of arachidonic acid , were markedly inhibited in cells treated with as little as 10 nM dexamethasone a concentration that only partially inhibited hydrolysis of inositol phospholipids or release of secretory granules . ^^^ Prolonged exposure to dexamethasone also resulted in a partial decrease in expression of MEK 1 , p42mapk , and cytosolic phospholipase A 2 , which may contribute further to the effects of dexamethasone on this pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
EGF induced CYP11A1 promoter activity was reduced 60 % by the MEK 1 inhibitor PD 098059 and 50 % by a dominant negative mutant of the ERK specific regulator MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To address these questions , we have utilized a constitutively active version of the immediate upstream activator of both ERK 1 and ERK 2 , mitogen activated / extracellular signal regulated kinase 1 ( MEK 1 ) , to activate ERK signaling selectively in the absence of other TCR activated signaling pathways . ^^^ The effect of constitutive MEK / ERK activation on T cell cytokine production was measured by transiently co transfecting newly activated mouse T cells with DNA encoding constitutively active MEK 1 ( CA MEK 1 ) and the human interleukin 2 ( IL 2 ) receptor alpha chain ( hCD 25 ) , purifying hCD25+ transfectants by flow cytometric cell sorting , and measuring the production of IL 3 , IL 4 , interferon ( IFN ) gamma and granulocyte / macrophage colony stimulating factor ( GM CSF ) either in the presence or absence of ionomycin stimulation . ^^^ CA MEK 1 expression led to constitutive ERK activation , which acted synergystically with ionomycin treatment to stimulate cytokine production . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Dominant negative mutants of either MEK 1 , ERK or SEK 1 , reduced small t dependent induction of the cyclin D 1 promoter . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 and MEK 2 phosphorylate a majority of the ERK 2 mutants . ^^^ Alteration of the residue between the two phosphorylation sites neither dramatically affected the activity of MEK 1 and MEK 2 toward ERK 2 nor conferred recognition by other MEKs . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
HeLa cell infection with L . monocytogenes EGD resulted in a rapid , but transient , phosphorylation of the MAP kinases erk 1 and erk 2 , a transient phosphorylation of the MAP kinase kinase MEK 1 , and a transient activation of the MAP kinase kinase kinase Raf . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of tyrosine kinase activity with genistein ( 10 microg / ml ) as well as selective MAP kinase inhibition with the MEK 1 inhibitor PD 98059 abolished the VLA 4 dependent ERK tyrosine phosphorylation , inhibited NF kappaB nuclear binding , and abrogated tissue factor expression induced by both VLA 4 cross linking and adhesion to fibronectin in THP 1 cells and human peripheral blood monocytes . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A two plasmid bacterial expression system was employed to express active forms of the following MEK and MAP kinase family members : ERK 1 , ERK 2 , alpha SAPK , and p 38 and their upstream activators , MEK 1 , 2 , 3 , and 4 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In the present study , we used a constitutively active MAPK / extracellular signal regulated kinase ( ERK ) kinase 1 ( MEK 1 ) mutant to investigate the function of the highly conserved MEK 1 ERK2 signaling module in renal epithelial cell differentiation and proliferation . ^^^ Stable expression of constitutively active MEK 1 ( CA MEK 1 ) in epithelial MDCK C 7 cells led to an increased basal and serum stimulated ERK 1 and ERK 2 phosphorylation as well as ERK 2 activation when compared with mock transfected cells . ^^^ In both mock transfected and CA MEK 1 transfected MDCK C 7 cells , basal and serum stimulated ERK 1 and ERK 2 phosphorylation was almost abolished by the synthetic MEK inhibitor PD 098059 . ^^^ Increased ERK 2 activation due to stable expression of CA MEK 1 in MDCK C 7 cells was associated with epithelial dedifferentiation as shown by both a dramatic alteration in cell morphology and an abolished cytokeratin expression but increased vimentin expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The selective MAPK / extracellular signal regulated kinase 1 ( MEK 1 ) inhibitor PD 098059 inhibited activation of p42MAPK induced by Fc gamma R cross linking , but not p 38 or JNK / SAPK activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 is required for PDGF induced ERK activation and DNA synthesis in tracheal myocytes . ^^^ We tested whether activation of mitogen activated protein kinase / extracellular signal regulated kinase kinase 1 ( MEK 1 ) is required and sufficient for extracellular signal regulated kinase ( ERK ) activation in airway smooth muscle cells . ^^^ First , we transiently cotransfected bovine tracheal myocytes with an epitope tagged ERK 2 and a dominant negative or a constitutively active form of the gene encoding MEK 1 and assessed ERK 2 activation by in vitro phosphorylation assay . ^^^ Expression of the dominant negative MEK 1 inhibited platelet derived growth factor ( PDGF ) induced ERK 2 activation , whereas expression of the constitutively active MEK 1 induced ERK 2 activation , suggesting that MEK 1 is required and sufficient for ERK activation in these cells . ^^^ Next , we assessed the effect of PD 98059 , a synthetic MEK inhibitor , on PDGF induced MEK 1 and ERK activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A beta ( 1 40 ) induced phosphorylation of p 44 and p 42 MAP kinases ( Erk 1 and Erk 2 ) at tyrosine 204 , and PD 98059 , a MEK 1 inhibitor , inhibited A beta ( 1 40 ) induced CREB phosphorylation at serine 133 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Western blot analysis was performed using anti ERK 2 , anti MEK 1 , and anti MEK 2 antibodies . ^^^ RESULTS : Corneal tissue expresses ERK 2 or MAPK , and both MEK 1 and MEK 2 , the immediate upstream regulators of MAPK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Interfering with MEK 1 , which is an upstream activator of ERK 1 , either with PD 098059 , which prevents the activation of MEK 1 , or with a dominant negative expression construct , reduced 92 kDa gelatinolysis and MMP 9 promoter activity respectively . c Raf 1 is an upstream activator of MEK 1 and a kinase deficient c Raf 1 expression construct decreased the activity of a promoter driven by either the MMP 9 promoter or three tandem AP 1 repeats . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Map / Erk kinase 1 ( MEK 1 ) and MEK 2 activate the Erk / MAP kinases and have been implicated in cell growth and differentiation . ^^^ These findings suggest that MEK 2 may be the primary Erk / MAP kinase activator during development and that MEK 1 may play a role in the proliferative or mitogenic response in adult mouse tissues . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The present study uses p 42 MAPK , also known as ERK 2 , and MEK 1 as representatives of their respective classes of enzymes . ^^^ We find that increasing the ERK 2 concentration decreases the rate of activation by a mechanism which does not involve inhibition of MEK 1 function . ^^^ Therefore , the mechanism of ERK 2 activation by MEK 1 in vitro is nonprocessive . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These results indicate that p21ras and MEK 1 are required for IFN gamma induced ERK 1 and ERK 2 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MAPK expression was determined in five human tumors and five normal tissues ( adjacent non neoplastic liver ) by Western blotting using specific antisera raised against four MAPK pathway intermediates : Erk 1 , Erk 2 ( extracellular signal regulated kinases ) , Mek 1 and Mek 2 ( mitogen activated protein kinase kinases ) . ^^^ There was a significant increase in Erk 1 , Erk 2 , Mek 1 and Mek 2 expression in particulate and cytosolic fractions prepared from tumor specimens as compared with the adjacent normal control tissues . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Interestingly , integrin mediated Erk 2 , Mek 1 , and Raf 1 activation , but not Ras GTP loading , was inhibited at least 80 % by wortmannin and LY 294002 . ^^^ We conclude that integrin mediated adhesion to fibronectin results in the accumulation of the PI 3 kinase products PI ( 3 , 4 ) P 2 and PI ( 3 , 4 , 5 ) P 3 as well as the PI 3 kinase dependent activation of the kinases Raf 1 , Mek 1 , Erk 2 , and AKT and that PI 3 kinase may function upstream of Raf 1 but downstream of Ras in integrin activation of Erk 2 MAP and AKT kinases . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of PDGF induced ERK 1 activity by the addition of a selective inhibitor of MEK 1 ( MAP kinase kinase / ERK kinase 1 ) activation , PD 98059 , or transfection with a dominant negative ERK 1 ( dnERK ) was correlated with growth arrest . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Morphine enhanced the cellular levels of ERK 1 ( 44 kDa ) , ERK 2 ( 42 kDa ) , a 54 kDa ERK , MEK 1 ( 45 kDa ) , and MEKK ( 78 kDa ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Overexpression of wild type ERK 1 and ERK 2 or activation of endogenous ERKs using activated MEK 1 ( mitogen activated protein kinase kinase or ERK kinase ) overexpression stimulated HDC promoter activity in a dose dependent fashion . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We confirm activation of the MAPK family members extracellular signal regulated kinases 1 and 2 ( ERK 1 and ERK 2 ) , p 38 , and Jun N terminal kinase / stress activated protein kinase ( JNK / SAPK ) , as well as activation of the immediate upstream MAPK activators MAPK / ERK kinases 1 and 4 ( MEK 1 and MEK 4 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Examination of ERK pathway kinases shows that neither MEK 1 nor Ras will synergize with Rho type proteins , and that only MEK 1 is fully activated , indicating that MEKs are a focal point for cross cascade regulation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor PD 98059 abrogated asbestos induced apoptosis , confirming a causal relationship between ERK activation and apoptosis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In tumor , extracellular regulated kinases ( ERKs ) ERK 1 , ERK 2 , and mitogen activated ERK regulated kinase 1 ( MEK 1 ) were elevated by three to fourfold as compared with adjacent nontumorigenic normal liver . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , specific inhibition of the RAF / ERK pathway by PD 98059 ( MEK 1 inhibitor ) completely blocked ERK activation by EGF and basal cell growth but not EGF stimulated growth , thereby dissociating the growth promoting roles of each pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Transient transfection of these PKC mutants into Cos 7 cells showed that members of all three groups of PKC ( conventional , novel , and atypical ) are able to activate p 42 MAPK as well as its immediate upstream activator , the MAPK / ERK kinase MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , the known cytoplasmic substrates for MEK 1 , ERK 1 , and ERK 2 are not required for this process . ^^^ Interestingly , we find a Golgi associated ERK , which we propose as the likely target for MEK 1 in Golgi fragmentation . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of the Erk 2 pathway by PD 98059 , specific for the upstream MAP kinase kinase ( MEK 1 ) , abolishes TPA stimulated junB but not insulin induced c jun . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK 2 is activated via a kinase cascade initiated by activation of the G protein p21Ras followed by phosphorylation and activation of Raf 1 and mitogen activated protein kinase kinase 1 ( MEK 1 ) . ^^^ In the present paper , we studied in human primary T cells the possible involvement of the Raf 1 / MEK 1 / ERK 2 pathway upon stimulation by jacalin , a mitogenic lectin which specifically stimulates CD4+ lymphocytes . ^^^ Moreover , activation of this pathway appeared to be essential , since its blockade by a specific inhibitor of the MEK 1 kinase abolished IL 2 gene transcription ; in contrast , in T cells stimulated with phytohemagglutinin M ( PHA ) , another potent T cell mitogenic lectin , blockade of MEK 1 reduced but did not totally inhibit either ERK 2 phosphorylation or IL 2 mRNA expression . ^^^ This shows , as already suggested , that another pathway in addition to the Raf 1 / MEK 1 / ERK 2 kinase cascade could be triggered in T cell activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Listeria monocytogenes invasion of epithelial cells requires the MEK 1 / ERK 2 mitogen activated protein kinase pathway . ^^^ These results suggest that MEK 1 and ERK 2 activities are essential for L . monocytogenes invasion into host epithelial cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , several lines of evidence suggest that CD 40 and the B cell Ag regulate ERK through distinct pathways that converge at the level of MEK 1 , mitogen activated protein kinase kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The first approach utilized transient expression of a catalytically inactive form of mitogen activated / ERK 1 ( CI MEK 1 ) , while the second involved using the MEK 1 and MEK 2 specific inhibitor PD 98059 to block ERK activation by the TCR . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Lastly , we observed that the use of the MEK 1 inhibitor PD 98059 inhibited TPA mediated ERK activity and abrogated the anti apoptotic effects of TPA . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 ( 80 micromol / L ) , a selective inhibitor of mitogen activated protein kinase / ERK kinase 1 ( MEK 1 ) , decreased ERK1 / 2 activity in injured arteries and also reduced the medial cell replication . ^^^ These findings demonstrate that fibroblast growth factor 2 induced ERK1 / 2 activation promotes medial cell replication after balloon injury ; however , signaling of intimal cell replication may not be linked to the MEK 1 dependent ERK pathway . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Phosphorylation of the 44 and 42 kDa proteins by one trial conditioning was inhibited by pretreatment with PD 098059 , A MEK 1 ( ERK Activating kinase ) inhibitor . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mitogens induce the sequential activation of the kinases Raf 1 > Mek 1 > Erk 2 > Rsk via the G protein Ras . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The Mek 1 dual specificity protein kinase phosphorylates and activates the mitogen activated protein kinases Erk 1 and Erk 2 in response to mitogenic stimulation . ^^^ However , elevated Mek ER activity attenuated subsequent stimulation of Erk 1 and Erk 2 by serum . 4 OH tamoxifen stimulation of Mek ER expressing fibroblasts also resulted in up regulation of cyclin D 1 expression and down regulation of p 27 ( Kip 1 ) expression , establishing a direct link between Mek 1 and the cell cycle machinery . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK activity was inhibited either by PD 98059 , a synthetic inhibitor of mitogen activated protein kinase ( MAPK ) / ERK kinase ( MEK ) , the upstream signaling intermediate required and sufficient for ERK activation , or by transient transfection with a dominant negative mutant of MEK 1 ( MEK 2A ) . ^^^ The level of activated ERKs was increased by transient transfection with either a constitutively active form of MEK 1 ( MEK 2E ) or wild type ERK 2 ( MAPKwt ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Protein kinase A ( PKA ) was found to enhance the activity by about eightfold , whereas both ERK ( extracellular signal regulated kinase ) activator kinase 1 ( MEK 1 ) and MEK kinase 1 ( MEKK 1 ) significantly repressed the activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To investigate further , we then purified a number of different kinases , including PKC , PhK , ZAP 70 , ERK , and MEK 1 ( a MKK ) , and showed that Ro 09 2210 was a selective inhibitor of MEK 1 in vitro ( IC 50 = 59 nM ) . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We find that co expression with caveolin 1 dramatically inhibits signaling from EGF R , Raf , MEK 1 and ERK 2 to the nucleus . ^^^ Peptides derived from this region of caveolin 1 also inhibit the in vitro kinase activity of purified MEK 1 and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We further demonstrate that 5 HT 1 agonists inactivate ERK by dephosphorylation , even in the presence of constitutively activated MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition is selective for MEK 1 and 2 , as U 0126 shows little , if any , effect on the kinase activities of protein kinase C , Abl , Raf , MEKK , ERK , JNK , MKK 3 , MKK 4 / SEK , MKK 6 , Cdk 2 , or Cdk 4 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 proline rich insert is required for efficient activation of the mitogen activated protein kinases ERK 1 and ERK 2 in mammalian cells . ^^^ MEK 1 and MEK 2 contain a proline rich insert not present in any other known MEK ( MAP ( mitogen activated protein ) / ERK ( extracellular signal regulated kinase ) kinase ) family members . ^^^ Overexpression of either constitutively active MEK 1 lacking the insert or ERK 2 compensates for the weaker in vivo activity of the MEK 1 deletion mutant . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Exposure to nerve growth factor , or expression of constitutively active MEK 1 two treatments which cause differentiation of PC 12 cells into a neuronal phenotype result in activation of ERK type MAP kinases and phosphorylation of c Jun on several sites including Ser 63 and Ser 73 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Addition of heptachlor to human CEM x 174 lymphocytic cells reduced the cellular levels of MAP kinase ( MAPK , mitogen activated protein kinase ) cascade proteins , including ERK 1 ( a 44 kDa MAPK ) , ERK 2 ( a 42 kDa MAPK ) , a 85 kDa and a 54 kDa MAP kinase , MEK 1 ( a 45 kDa ERK kinase ) and MEKK ( a 78 kDa MEK kinase ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To further assess the importance of sustained ERK activity in cellular differentiation , we transiently transfected a mutant constitutively active MEK 1 construct into AU 565 cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 inhibition resulted in lower levels of ERK 2 activation and synthesis and higher levels of apoptosis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We compared the distribution of p 42 mitogen activated protein kinase ( p42MAPK ) and its activator MAPK or ERK kinase ( MEK 1 ; involved in transduction of growth and differentiation signals ) , with c Jun N terminal kinase ( JNK 1 ) and its activator MEK 4 ( involved in transduction of stress and death signals ) in the adult rat central nervous system . ^^^ Immunolocalization of the mitogen activated protein kinases p42MAPK and JNK 1 , and their regulatory kinases MEK 1 and MEK 4 , in adult rat central nervous system . ^^^ A high degree of correspondence was found between the regional distribution of MEK 1 and p42MAPK . ^^^ Immunostaining for MEK 1 and p42MAPK was intense in olfactory structures , neocortex , hippocampus , striatum , midline , and interlaminar thalamic nuclei , hypothalamus , brainstem , Purkinje cells , and spinal cord . ^^^ The distribution of MEK 1 and p42MAPK proteins only partially overlapped with that of MEK 4 and JNK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 098059 , a specific MEK 1 inhibitor , also blocked ERK activation by DA . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In vitro protein kinase assays demonstrated that activation of Raf 1 and B Raf resulted in the phosphorylation and activation of MAPK kinase ( MEK 1 ) , which subsequently phosphorylated p42mapk . ^^^ Additionally , both PGF2alpha ( 1 microM ) and PMA ( 20 nM ) stimulated phosphorylation of Raf 1 , MEK 1 , and p42mapk in 32P labeled cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Expression of constitutively active MEK 1 , the kinase that activates ERKs , or overexpression of ERK 2 , but not JNK 1 , inhibited Stat 3 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A protein called MP 1 ( MEK Partner 1 ) was identified that bound specifically to MEK 1 and ERK 1 and facilitated their activation . ^^^ Expression of MP 1 in cells increased binding of ERK 1 to MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , in normal T cells , inhibition of PI 3 kinase prevented activation of enzymes in the extracellular signal regulated protein kinase ( ERK ) signaling pathway ( MEK 1 and ERK 2 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Most of the mitogenic protein kinases including Raf 1 , RafB , Mek 1 , Erk 2 , and Rsk 1 were significantly down regulated , whereas the stress signaling kinases , such as Mlk 3 , Mekkl , Sekl , Mkk 3 , and Mapkapk 2 were up regulated in expression during postnatal development . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In vitro , it activated MAP / extracellular signal regulated protein kinase ( ERK ) kinases ( MEKs ) 3 , 4 , and 6 of the stress responsive MAP kinase pathways , but not MEK 1 or 2 of the classical MAP kinase pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : To create an activated ERK 2 variant , we fused ERK 2 to the low activity form of its upstream regulator , the MAP kinase kinase MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 S218A , S222A does not inhibit MEK phosphorylation and is a poor inhibitor of ERK phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Convergence of CD 19 and B cell antigen receptor signals at MEK 1 in the ERK 2 activation cascade . ^^^ The best defined pathway , known to be activated by mIgM , consists of the sequential activation of the mitogen activated protein kinase ( MAPK ) cascade that includes Ras , Raf , MAPK kinase 1 ( MEK 1 ) , and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , we found that LPS activated the extracellular signal regulated kinase ( ERK ) , and that specific inhibition of MEK 1 , the kinase which activates ERK , abrogated the ability of LPS to prevent apoptosis but did not inhibit DC maturation or NF kappaB nuclear translocation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Both IL 8 and GROalpha activate ERK and MEK through R 2 , whereas MIP 1alpha , a beta chemokine , does not activate these kinases through either of these receptors . ( b ) ERK activation is inhibited by pertussis toxin and MEK 1 inhibitor . ( c ) ERK activation is independent of the upstream mediators Ras and Raf 1 . ( d ) The downstream effects of ERK activation result in an increase of c fos mRNA through both R 1 and R 2 receptors . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To investigate the contribution that ERK / mitogen activated protein kinase signalling makes to cell cycle progression and gene expression , we have constructed cell lines to express an inducible version of activated MEK 1 . ^^^ Activation of MEK 1 in growth arrested cells leads to DNA synthesis ; however , ERK activation alone is insufficient because the induction of DNA synthesis is blocked by inhibition of phosphatidylinositol 3 kinase ( PI 3 kinase ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here , we report that ERK 2 activated by its upstream kinase , MEK 1 , represses Stat 3 transcriptional activity induced by Src or Jak 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK activation in the presence of cAMP did not appear to involve any of the Raf isoforms and was blocked by expression of dominant negative MEK 1 or treatment with a MEK inhibitor , PD 98059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Induction of MMP 13 expression was inhibited by treatment of fibroblasts with a specific p 38 inhibitor , SB 203580 , whereas blocking the ERK 1 , 2 pathway ( Raf / MEK1 , 2 / ERK1 , 2 ) by PD 98059 , a selective inhibitor of MEK 1 , 2 activation potently augmented MMP 13 expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 098059 , a selective inhibitor of MAPK kinase 1 ( MEK 1 ) , blocked activation of extracellular signal regulated kinase ( ERK ) and partially blocked TNF alpha production by the clone . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor , PD 98059 , caused a dose dependent reduction in ERK 2 activity that paralleled a decrease in migration up to 60 % . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MAPK kinase ( MEK 1 ) inhibitor PD 98059 partially inhibits Erk phosphorylation and does not inhibit chromaffin cell proliferation , while depolarization selectively inhibits proliferation without blocking Erk phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This extracellular signal regulated protein kinase ( ERK ) activation was inhibited successfully by the MEK 1 inhibitor PD 98059 , but not by the estrogen receptor ( ER ) antagonist ICI 182 , 780 , and did not appear to result from estradiol induced activation of trk . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
TPO did not activate the mitogen activated protein kinase / ERK kinases , MEK 1 and MEK 2 , but activated Raf 1 and directly augmented the PKC mediated MEK activation , suggesting that TPO primarily potentiates the ERK pathway through regulating MEKs or upstream steps of MEKs including Raf 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK activation follows phosphorylation by a class of specific upstream MAP kinase / ERK kinase ( MEK ) exemplified by MEK 1 . ^^^ We report here the development of a simple , 96 well plate , quantitative in vitro assay measuring purified ERK 2 catalytic activation by a constitutive MEK 1 mutant ( S218E S222E ) . ^^^ This simple assay allows several hundred quantitative measurements of MEK 1 dependent ERK 2 activation to be performed in a day . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Therefore , MEK 1 participates in an obligate signaling pathway linking CSF 1R to c Myc expression , but other signals from CSF 1R must cooperate with the MEK / ERK pathway to induce c Myc expression and S phase entry in response to CSF 1 stimulation . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
When overexpressed in mammalian cells , wild type PTPBR 7 suppressed the phosphorylation and activation of ERK by epidermal growth factor ( EGF ) , nerve growth factor ( NGF ) , and constitutively active MEK 1 , a mutant MAPK kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
By use of Western blotting for iNOS detection and ELISA for quantitation of TNF secretion , three selective inhibitors of these pathways were tested ( the p 38 inhibitors SB 202190 and SB 203580 and the MEK 1 , 2 / ERK inhibitor PD 98059 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The components of this assay include human cRaf 1 , MEK 1 , and ERK 2 and a biotinylated peptide substrate for ERK 2 . cRaf 1 was expressed as a his tagged protein in insect cells in an active form . ^^^ MEK 1 and ERK 2 were expressed in Escherichia coli as glutathione S transferase ( GST ) fusion proteins in their inactive forms . ^^^ When the purified components are incubated together , cRaf 1 phosphorylates and activates MEK 1 , MEK 1 phosphorylates and activates ERK 2 , and ERK 2 phosphorylates the peptide , biotin AAATGPLSPGPFA . ^^^ The assay detects inhibitors of cRaf 1 , MEK 1 , or ERK 2 , and has been used to screen large numbers of compounds . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , ATF 1 had no effect in F 9 cells and exhibited a dominant negative effect in SK N MC cells . bFGF stimulation led to phosphorylation of the p 38 mitogen activated protein kinase ( MAPK ) , and the extracellular signal regulated kinase ( ERK ) MAPK and promoter activation , phosphorylation of CREB , and GAL 4 CREB dependent transcription were selectively prevented by a dominant negative Ras mutant , the p 38 MAPK specific inhibitor SB 203580 , and the MAP / ERK kinase 1 ( MEK 1 ) inhibitor PD 098059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 098059 , a selective inhibitor of the ERK activating kinase MEK 1 , had no effect on IL 1beta induced MCP 1 mRNA or protein levels , or on IL 1beta activation of NF kappaB . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MKK 3 and MKK 6 are known potential constituents of p 38 MAPK signaling pathway , whereas SEK 1 and MEK 1 are upstream activators of SAPK / JNK and ERK pathways , respectively . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Transient transfection of a dominant interfering mutant of mitogen activated / extracellular signal regulated receptor protein kinase kinase ( MEK 1 ) demonstrated that down regulation of the ERK pathway inhibited FasL expression during AICD , whereas activation of the ERK pathway with a constitutively active MEK 1 resulted in increased expression of FasL . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , TGF beta 1 does not rescue serum deprivation induced apoptosis in RAW 264 . 7 cells transfected with a dominant negative mutant MAPK ( ERK 2 ) cDNA or in wild type RAW 264 . 7 cells in the presence of the MAPK kinase ( MEK 1 ) inhibitor . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
By using the mitogen activated protein kinase / ERK 1 ( MEK 1 ) inhibitor PD 98059 and PI3K inhibitors wortmannin and LY 294002 , we found that blocking the MEK 1 ERK pathway had no effect on HRG pbeta 1 enhanced cell aggregation ; however , blocking the PI3K pathway greatly inhibited HRG beta 1 mediated cell aggregation . ^^^ Our study indicated that the HRG beta 1 activated MEK 1 ERK pathway has no demonstrable role in the induction of cell aggregation , whereas HRG beta 1 activated PI3K is required for enhancing breast cancer cell aggregation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Cell migration assays indicated that mek 1 / fibroblasts could not be induced to migrate by fibronectin , although the levels of Mek 2 protein and Erk activation were normal . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , there was no inhibition observed in cells treated with specific MEK 1 inhibitor , PD 98059 , or with stable expression of a dominant negative mutant of ERK 2 or JNK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mek 1 and especially Mek 2 protein expression , as well as MAP kinase kinase activity as determined by phosphorylation of kinase inactive Erk [ GST K71A ] were increased in cancer samples . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To identify Raf 1 independent upstream signaling intermediates of mitogen activated protein kinase / ERK kinase 1 ( MEK 1 ) , the dual function kinase required and sufficient for ERK activation in these cells , lysates from forskolin and PDGF treated bovine tracheal myocytes were resolved using ion exchange chromatography . ^^^ The ability of these fractions to activate MEK 1 was confirmed by examining the phosphorylation of myelin basic protein , a known substrate for ERKs , in the presence of functional MEK 1 and ERK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , the selective MEK 1 inhibitor , PD 98059 , inhibits oestradiol and progestin stimulation of Erk 2 and the steroid dependent S phase entry . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To answer this question , a constitutively active form of mitogen activated Erk activating kinase ( MEK 1 ) was cotransfected with an NF M expression construct into NIH 3T3 cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In support of these data , we also show that MEK 1 is highly phosphorylated in vivo on Ser 217 / 221 in MLK 3 transformed fibroblasts , whereas activating ERK phosphorylations are barely detectable . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Further , the forced expression of active forms of MEK 1 and MKK 6 , which are the upstream kinases of ERK and p38MAPK , respectively , induced de differentiation even when SMCs were stimulated with IGF 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Active Ras and MAPK / ERK kinase 1 ( MEK 1 ) ( the upstream activator of ERK ) each induced cyclin D 1 promoter activity , whereas active stress activated protein kinase / ERK kinase 1 ( SEK 1 ) , an upstream activator of JNK , did not . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of ERK activity by a dominant negative MEK 1 mutant significantly attenuates beta arrestin 1 phosphorylation , thereby increasing the concentration of dephosphorylated beta arrestin 1 . ^^^ In contrast , if ERK mediated phosphorylation is increased by transfection of a constitutively active MEK 1 mutant , receptor internalization is inhibited . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MAPK 2 turns out to be a crucial mediator of the OSM effect in glioma cells since inhibition of MAPK activity by the Mek 1 inhibitor PD 98059 blocks the OSM induced inhibition of DNA synthesis by about 70 % . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This was confirmed in the current study by showing that activation of MEK 1 , the upstream regulator of ERK , reduces Fas mediated apoptosis , whereas inhibition of MEK 1 augments apoptosis by Fas . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We here have investigated this issue by using MAP kinase kinase ( MEK 1 ) inhibitor PD 098059 and a dominant negative Erk 2 , as well as wild type Erk 2 , in a JB 6 cell model . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of 1 ) protein kinase C ( PKC ) , 2 ) mitogen and extracellular signal regulated kinase kinase ( MEK 1 ) with 2 ' amino 3 ' methoxyflavone ( PD 90859 ) , and 3 ) extracellular signal regulated kinase ( ERK ) with apigenin attenuated this effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In a previous study , we have demonstrated that a constitutively active form of MEK 1 activates Erk 1 and Erk 2 kinases , which phosphorylate co transfected NF M in NIH 3T3 cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The present study analyzed the effect of glucocorticoids on key regulatory pathways leading to passage of cells through the restriction point of the cell cycle , including those mediated by extracellular regulated kinases ( ERK ) 1 and 2 ; the ERK upstream regulator MAPK kinase ( MEK 1 ) ; cyclin D 1 levels ; and levels and phosphorylation of retinoblastoma protein ( pRb ) . ^^^ PD 98059 ( 10 microM ) , an inhibitor of MEK 1 activation , markedly attenuated thrombin stimulation of ERK activity and phosphorylation , DNA synthesis , and cyclin D 1 levels . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Using the Jurkat T lymphocyte cell line , we found that a stably expressed Gi protein coupled receptor ( the delta opioid receptor ( DOR 1 ) ) stimulates MEK 1 and extracellular signal regulated kinases 1 and 2 ( ERK 1 and ERK 2 ) and transcriptional activity by an ERK target , Elk 1 , via a mechanism requiring a PTX sensitive Gi protein . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Expression of wild type LC PTP in 293T cells suppressed the phosphorylation of ERK 2 by a mutant MEK 1 , which was constitutively active regardless of upstream activation signals . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment with ebselen , catalase , and the flavoprotein inhibitor diphenylene iodonium each attenuated PDGF and Rac 1 mediated cyclin D ( 1 ) promoter activation , while having no effect on the induction of cyclin D ( 1 ) by mitogen activated protein kinase / extracellular signal regulated kinase ( ERK ) kinase 1 ( MEK 1 ) , the upstream activator of ERKs . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 098059 , an inhibitor of the Erk 1 / 2 upstream kinase mitogen activated protein kinase kinase 1 ( MEK 1 ) , blocked Erk 1 / 2 activation , Egr 1 induction , and neuronal death by zinc . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Evidence for a p 21 ( ras ) / Raf 1 / MEK 1 / ERK 2 independent pathway in stimulation of IL 2 gene transcription in human primary T lymphocytes . ^^^ We found that , in contrast to the commonly used UCHT 1 , 10 35 activated IL 2 gene transcription without stimulation of the Raf 1 / mitogen activated ERK kinase 1 ( MEK 1 ) / ERK 2 phosphorylation cascade ; we also showed that 10 35 stimulation , which triggers an ERK 2 independent pathway , does not involve activation of p 21 ( ras ) . ^^^ In addition to demonstrating that activation of p 21 ( ras ) and of its Raf 1 / MEK 1 / ERK 2 effector pathway is not an event obligatorily triggered upon TCR / CD3 ligation , these results provide the first evidence of the existence of a p 21 ( ras ) / ERK 2 independent pathway for IL 2 gene transcription in human primary T lymphocytes . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Although the MAPK ( ERK ) pathway is activated following infection with EBV , MAPK / ERK kinase ( MEK 1 ) activity is not required to drive the proliferation of infected cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Raf 1 phosphorylates and activates MEK 1 , a kinase that activates the extracellular signal regulated kinases ( ERK ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A specific inhibitor ( PD 98059 ) of MEK 1 , the kinase that activates ERK , had little or no effect on PDT induced apoptosis in either LY R or CHO cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , coexpression of the GFP ERK 2 with its upstream activator , MEK 1 , resulted in a cytosolic retention of the GFP ERK 2 , which was the result of its association with MEK 1 , and was reversed upon stimulation . ^^^ Substitution of residues 312 319 of GFP ERK 2 to alanine residues prevented the cytosolic retention of ERK 2 as well as its association with MEK 1 , without affecting its activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition to requirements for PI3K , PDK 1 , and PKC zeta , we found that a tyrosine kinase ( presumably the insulin receptor ) , the SH 2 domain of GRB 2 , SOS , RAS , RAF , and MEK 1 were required for insulin effects on ERK in the rat adipocyte . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 ( MAP kinase / ERK kinase ) / ERK ( extracellular signal responsive kinase ) pathway has been implicated in cell growth and differentiation [ Seger , R . & Krebs , E . ^^^ This study indicates that the MEK1 / ERK pathway contributes to brain injury during focal cerebral ischemia and that PD 98059 , a MEK 1 specific antagonist , is a potent neuroprotective agent . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Thus , H . pylori rapidly activates ERK , p 38 , and JNK MAP kinases in gastric epithelial cells ; cag+ isolates are more potent than cag strains in inducing MAP kinase phosphorylation and gene products of the cag pathogenicity island are required for maximal MAP kinase activation . p 38 and MEK 1 activity are required for H . pylori induced IL 8 production , but do not appear to be essential for H . pylori induced NF kappaB activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
When ectopically expressed , the serine / threonine kinase Mos can induce oncogenic transformation of somatic cells by direct phosphorylation of MAP kinase / ERK kinase ( MEK 1 ) , activating the mitogen activated protein kinases ERK 1 and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The N terminal ERK binding site of MEK 1 is required for efficient feedback phosphorylation by ERK 2 in vitro and ERK activation in vivo . ^^^ An ERK 2 binding site at the N terminus of MEK 1 was reported to mediate their stable association . ^^^ We examined the importance of this binding site in the feedback phosphorylation of MEK 1 on Thr ( 292 ) and Thr ( 386 ) by ERK 2 , the phosphorylation and activation of ERK 2 by MEK 1 , and the interaction of MEK 1 with ERK 2 and Raf 1 . ^^^ Deletion of the binding site from MEK 1 reduced its phosphorylation by ERK 2 , but had no effect on its phosphorylation by p 21 activated protein kinase 1 ( PAK 1 ) . ^^^ A MEK 1 N terminal peptide containing the binding site inhibited MEK 1 phosphorylation by ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These results indicate that TGF beta 1 specifically activates MEK 1 and subsequent ERK pathways in CRAC , and that the activation of this MAPK pathway plays a role in the mitogenic response to TGF beta1 . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibitors of ERK activation , including PD 098059 and dominant negative MEK 1 , also decreased the migration of LRP deficient but not control cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , nuclear extracts from cells exposed to PD 98059 , a highly selective inhibitor of MAP kinase ERK kinase 1 ( MEK 1 ) and MEK 2 , showed reduced binding . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , expression of constitutively activated MEK 1 or its downstream target Erk 2 MAP kinase was sufficient to stimulate TGF alpha shedding . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Treating the cells with p 38 inhibitor SB 202190 inhibited p 38 activation , but not ERK ; treating the cells with MEK 1 inhibitor PD 98059 inhibited ERK activation without suppressing p 38 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A docking domain in extracellular signal regulated kinase ( ERK ) , a MAPK , serves as a common site for binding to the MAPK kinase MEK 1 , the MAPK activated protein kinase MNK 1 and the MAPK phosphatase MKP 3 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of ERK activation by PD 98059 , a specific inhibitor of the ERK signaling pathway , blocked the osteogenic differentiation in a dose dependent manner , as did transfection with a dominant negative form of MAP kinase kinase ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Unlike ERK 1 and ERK 2 , ERK1b failed to interact with MEK 1 as judged from its nuclear localization in resting cells overexpressing ERK1b together with MEK 1 or by lack of coimmunoprecipitation of the two proteins . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Using these mutant variants of MEK 1 , we showed previously that transfection of NIH / 3T3 or Swiss 3T3 cells causes morphological transformation and increases growth on soft agar , independent of ERK activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Therefore , most EGF stimulated Sp 1 kinase activity is Mek 1 dependent and distinct from Erk . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To further examine the role of ERK in negative selection we used the MEK 1 inhitibor , PD 98059 , a specific pharmacological inhibitor of the ERK pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activated MAP kinases ( ERK 1 and ERK 2 ) were detected in deltaRaf : ER transformed cells , and their presence was dependent upon a functional MEK 1 protein . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
EC migration and proliferation stimulated by basic fibroblast growth factor ( FGF 2 ) were blocked by inhibition of ERK activity by both the specific mitogen activated protein kinase kinase ( MEK ) 1 inhibitor PD 98059 and the overexpression of a dominant negative mutant of MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Moreover , KSR reconstituted mitogen activated protein kinase activation was detected in KSR immunoprecipitates depleted of all contaminating kinases ( c Raf 1 , MEK 1 , ERK 2 ) by multiple high salt washes . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Extracellular signal regulated kinase ( ERK ) 1 and ERK 2 , the known downstream targets of MEK 1 , are not required for this fragmentation ( Acharya et al . 1998 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , MEK 1 activation is required for ONOO ( ) induced ERK activation in myofibroblasts . ^^^ In contrast , H ( 2 ) O ( 2 ) induced ERK activation is dependent on EGFR activation , which then leads to downstream Raf 1 and MEK 1 activation . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In healthy subjects , within 30 min , insulin significantly increased MAP kinase [ isoforms p 42 ( MAPK ) and p 44 ( MAPK ) ( ERK 1 and ERK 2 ) ] phosphorylation ( 141 + / 2 % , P < 0 . 05 ) and activity ( 177 + / 5 % , P < 0 . 05 ) , and the activity of its upstream activator MEK 1 ( 161 + / 16 % , P < 0 . 05 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A dominant negative mutant of B Raf , but not of Raf 1 , blocked the cAMP induced activation of ERK , indicating that B Raf is the MEK 1 upstream regulator mediating this cAMP effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , specific activation of ERK 1 , 2 by adenovirus mediated expression of constitutively active MEK 1 in dermal fibroblasts results in marked reduction in decorin mRNA abundance and production . ^^^ Co transfections of human decorin promoter 5 ' deletion constructs with constitutively active MEK 1 expression vector identified the region 278 to 188 as essential for ERK 1 , 2 mediated down regulation of decorin promoter activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
AP 1 binding was dependent on ERK activation , since the MEK 1 ( mitogen activated protein kinase kinase ) inhibitor PD 98059 inhibited TGF beta 1 induced binding . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of c Src tyrosine kinase ( 4 amino 5 [ 4 chlorophenyl ] 7 [ t butyl ] pyrazolo [ 3 , 4 d ] pyrimidine ; PP 2 ) or MEK 1 ( mitogen activated protein kinase [ MAP ] / extracellular signal regulated kinase [ ERK ] 1 ) ( PD 98059 , blocking ERK1 / 2 ) blocked or reversed the pulsatile flow pH ( 1 ) change to acidification . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Serum starved cells and cells grown in medium with serum did not show constitutively activated c Raf , MEK 1 , or p 42 MAPK . ^^^ Stimulation of cells with epidermal growth factor ( EGF ) or fetal calf serum ( FCS ) resulted in activation of N Ras , but not K Ras , as well as activation of c Raf , MEK 1 , and p 42 MAPK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , both PD 98059 and a dominant negative ERK 2 augmented NF kappaB driven transcription ; however , neither PD 98059 nor MEK 1 altered NF kappaB activation at any level . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Association of decreased phosphorylation of ERK 2 with costimulation of rat T cell activation by MEK 1 inhibitors and TGF beta 1 . ^^^ ERK is phosphorylated by MEK 1 and PD 098059 and U 0126 are specific inhibitors for this kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , overexpression of MEK 1 DN in MCs inhibited endothelin 1 ( ET 1 ) , phenylephrine ( PE ) , leukemia inhibitory factor ( LIF ) , isoproterenol ( ISP ) , and mechanical stretch induced ERK activation and ANP mRNA expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
CD 19 consistently enhanced activation of ERK 2 and MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor PD 98059 blocked ERK 1 / 2 phosphorylation , and treatment of endothelial cells with PD 98059 resulted in apoptosis even upon Fg : ICAM 1 ligation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
TGFbeta 1 evoked Erk activation was blocked by the specific MEK 1 inhibitor 2 ( 2 ' amino 3 ' methoxyphenyl ) oxanaphthalen 4 one ( PD 98059 ) . ^^^ These results indicate that TGFbeta 1 elicits a biphasic stimulation of K ( Ca ) via activation of an MEK 1 Erk pathway and raise the possibility that other neuronal effects of TGFbeta superfamily members entail Erk activation . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Upregulation of MMP 1 expression by mitogenic or inflammatory stimuli is blocked by specific small molecular weight inhibitors of the ERK pathway or the p 38 pathway , respectively , and constitutively active kinases within the ERK1 / 2 pathway ( MEKK 1 , MEK 1 ) or the p 38 pathway ( ASK 1 , MEKK 1 , MKK 3 ) are potent activators of the MMP 1 promoter . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We found that switching off 5 Src led to a decrease in the activity of both PI 3 K and ERK1 / 2 , however , we found that adding a specific inhibitor of PI 3 K ( LY 294002 ) to 5 Src transformed Rat 1 cells grown in low serum induced apoptosis while a specific ERK kinase ( MEK 1 ) inhibitor ( PD 98059 ) had no effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To ensure that ERK activation was unnecessary for pVWF dependent platelet activation , we functionally inhibited ERK dependent signalling with PD 98059 , a potent and selective inhibitor of the MAP kinase kinase ( MEK 1 ) , which is the upstream kinase of ERK 1 and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ATP induced TNF alpha release was inhibited by PD 098059 , an inhibitor of extracellular signal regulated protein kinase ( ERK ) kinase 1 ( MEK 1 ) , which activates ERK , and also by SB 203580 , an inhibitor of p 38 mitogen activated protein kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Using PD 98059 , an inhibitor of the ERK kinase MEK 1 , we have here assessed the effects of ERK inhibition on the pattern of protein expression in the metastatic human breast cancer cell line MDA MB 231 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In the ovarian carcinoma cell line A 2780 , inhibition of ERK1 / 2 activation with the mitogen activated protein kinase / ERK kinase 1 ( MEK 1 ) inhibitor PD 98059 resulted in decreased p 53 protein half life and diminished accumulation of p 53 protein during exposure to cisplatin . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 ( mitogen activated ERK kinase ) inhibitor , PD 98059 ( 30 microM ) , inhibited both ERK phosphorylation and activity , and either prevented ( thrombin 0 . 3 and 3 u ml ( 1 ) , bFGF 300 pM ) or attenuated ( bFGF 3 nM ) DNA synthesis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
After mice were infected daily with 30 third stage larvae of A . cantonensis by oral adminstration for 6 weeks , no significant differences PKC alpha , MEK 1 , ERK 2 , JNK , and p 38 protein expression were found between the control and infected mice . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This same pattern in both cells of IRS 1 dependent augmentation and IRS 1 independent wortmannin sensitivity was also observed for GH induced activation of Akt and MEK 1 ( using state specific antibody blotting for both ) , despite the lack of difference in GHR , JAK 2 , SHP 2 , p 85 , Akt , Ras , Raf 1 , MEK 1 , ERK 1 , or ERK 2 abundance between the two cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In this study we demonstrate that endogenous MEKK 1 binds to endogenous ERK 2 , MEK 1 , and another MEKK level kinase , Raf 1 , suggesting that it can assemble all three proteins of the ERK 2 MAP kinase module . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK inhibitor U 0126 blocked the induction , while activated MEK 1 transfected into a rat mammary adenocarcinoma cell line induced a sustained activation of ERK and up regulated SMC / Muc4 expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Interference with ERK activation by either highly specific inhibitors of MEK 1 or a dominant negative ras mutant profoundly impaired the ability of EWS / FLI 1 to transform NIH3T3 cells to growth in semi solid medium . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : The MEK 1 inhibitor PD 98059 decreased erk 1 and 2 phosphorylation and blocked actin reorganization in a dose dependent manner . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Presently , we found that glucose and certain sugars rapidly activated extracellular signal regulated kinase ( ERK ) by a mechanism that was : ( a ) independent of glucose uptake / metabolism and protein kinase C but nevertheless cytochalasin B inhibitable ; ( b ) dependent upon proline rich tyrosine kinase 2 ( PYK 2 ) , GRB 2 , SOS , RAS , RAF , and MEK 1 ; and ( c ) amplified by overexpression of the Glut 1 , but not Glut 2 , Glut 3 , or Glut 4 , glucose transporter . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In the present study , we constructed a replication defective adenovirus vector carrying mutated form of MEK 1 ( CA MEK virus ) , which constitutively activate ERK pathway , and investigated its effect on thoracic spinal cord injury model in young adult rats as well as neurite outgrowth in vitro . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , expression of constitutively active MEK 1 , which activates ERK pathway , enhanced RXRE dependent activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In that study we also found MEK 1 and Raf 1 , which are involved in the extracellular signal regulated kinase ( ERK ) MAPK cascades , bind to JSAP 1 . ^^^ Finally , we investigated the molecular mechanism of JSAP 1 ' s inhibitory function and showed that JSAP 1 prevents MEK 1 phosphorylation and activation by Raf 1 , resulting in the suppression of the activation of ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pharmacological inhibition of MEK 1 or expression of a dominant negative form of c Raf or ERK 2 inhibits phenylephrine stimulated NFAT 3 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Moreover , the MAPK / ERK activation by HCV core protein was blocked in the presence of the specific MEK 1 inhibitor , PD 98059 . ^^^ These results indicate that ERK activation by HCV core protein may be independent of hepatocyte mitogen mediated signaling but synergistic with TPA , and HCV core protein may function at MEK 1 or farther upstream of that component . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The role of the mitogen activated protein kinase pathway was assessed using PD 98059 , which specifically inhibits mitogen activated protein kinase 1 and 2 ( that is , MEK 1 and MEK 2 ) , which activates mitogen activated protein kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Moreover , MGSA / GROalpha induction of NF kappaB is independent of the MEK1 / ERK cascade , because MGSA / GROalpha failed to increase ERK and ELK activation , and specific chemical inhibitors for MEK 1 ( PD 98059 ) had no effect on MGSA / GROalpha enhanced NF kappaB activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Preincubation with the MAP kinase kinase ( MEK 1 ) inhibitors PD 98059 ( 20 ng / ml ) and U 0126 ( 250 nM ) , or the PI 3 K inhibitors wortmannin ( 100 nM ) and LY 294002 ( 50 microM ) repressed the activation of ERK 1 , ERK 2 , and Akt in association with CPPD crystal incubation , in the absence or presence of TNF alpha . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Thus , coactivation of cAMP dependent pathways and ERK or JNK ( either through H Ras ( v 12 ) , Rac 1 ( v 12 ) , or MEK 1 ( S217E / S221E ) ) is inconsistent with cell survival . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The potential role of ERK in PGHS 2 up regulation was assessed by using cell lines expressing , both stably and after adenoviral infection , constitutively active forms of its upstream activator MAPK / ERK kinase ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Cytosolic MKP 3 was induced in parallel to ERK activation , the induction being dependent on ERK activation , as was shown using the MEK 1 inhibitor PD 98059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here we show that the corresponding sequences in human MEK 1 and MEK 2 are necessary and sufficient for the direct binding of the MAPKs ERK 1 and ERK 2 . ^^^ Furthermore , short peptides corresponding to the docking sites in these MEKs inhibited MEK 1 mediated phosphorylation of ERK 2 in vitro . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The protective effect of IGF 1 was blocked by phosphoinositide 3 kinase ( PI3K ) inhibitors , wortmannin , and LY 294002 , but was unaffected by rapamycin , PD 98059 , or SB 203580 , which inhibit mammalian target of rapamycin ( mTOR ) , ERK kinase ( MEK 1 ) , and p 38 MAPK respectively . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Finally , mechanistic studies to examine the mechanism by which alpha 1 antitrypsin acts , showed that alpha 1 antitrypsin induced the rapid activation of p42MAPK and p44MAPK ( also known as ERK1 / 2 ) and that the specific MEK 1 inhibitor PD 98059 totally blocked alpha 1 antitrypsin ' s mitogenic effects . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This suggests that laminin and fibronectin induced MEK 1 activation and the downstream targets Erk 1 and Erk 2 are involved in NF M KSP tail domain phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Rac 1 may stimulate the ERK cascade either by promoting the phosphorylation of c Raf or by increasing MEK 1 and / or 2 association with c Raf to facilitate MEK 1 and / or 2 activation . ^^^ However , toxin B decreased both the association of MEK 1 and / or 2 with c Raf and c Raf associated ERK activating activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PGF2alpha induced ERK phosphorylation was dose dependently inhibited by the MEK 1 inhibitor PD 098059 ( 1 50 microM ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Like ERK 1 and ERK 2 , ERK 5 is expressed in neurons , and ERK 5 stimulation by epidermal growth factor is blocked by the MAP kinase / ERK kinase 1 ( MEK 1 ) inhibitors PD 98059 and U 0126 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
LPS stimulation of peritoneal macrophages from these mice did not activate MEK 1 , ERK 1 , and ERK 2 but did activate JNK , p 38 MAPK , and NF kappaB . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 , a specific MAP kinase kinase ( MAPKK ) 1 ( also referred to as MEK 1 ) inhibitor , inhibited EGF dependent cell proliferation , that was associated with the inhibition of ERK and JNK phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A gain of function approach was used to perturb the ERK pathway in the lenses of transgenic mice via expression of a constitutively active mutant of the mitogen activated protein kinase kinase 1 ( MEK 1 ( E ) ) , the direct upstream kinase of the ERK 1 and ERK 2 kinases , under the alphaA crystallin promoter . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This targeting of ERK 2 reflects the formation of multiprotein complexes containing AT1aR , beta arrestin 2 , and the component kinases of the ERK cascade , cRaf 1 , MEK 1 , and ERK 2 . ^^^ Myc tagged cRaf 1 , MEK 1 , and green fluorescent protein tagged ERK 2 coprecipitated with Flag tagged beta arrestin 2 from transfected COS 7 cells . ^^^ Coprecipitation of cRaf 1 with beta arrestin 2 was independent of MEK 1 and ERK 2 , whereas the coprecipitation of MEK 1 and ERK 2 with beta arrestin 2 was significantly enhanced in the presence of overexpressed cRaf 1 , suggesting that binding of cRaf 1 to beta arrestin facilitates the assembly of a cRaf 1 , MEK 1 , ERK 2 complex . ^^^ The phosphorylation of ERK 2 in beta arrestin complexes was markedly enhanced by coexpression of cRaf 1 , and this effect is blocked by expression of a catalytically inactive dominant inhibitory mutant of MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Results were compared with those in T cells in which ras MAPK signaling was inhibited with a soluble inhibitor of MAPK ERK 1 ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Serum stimulation or constitutive activation of ERK kinase ( MEK 1 ) results in phosphorylation and cytoplasmic accumulation of hnRNP K . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RSK activity was reduced by PI 3 kinase inhibitor LY 294002 or MEK 1 inhibitor PD 98059 , suggesting that the ERK as well as the PI 3 kinase pathways are involved in regulation of RSK activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This enhanced ERK activation was sensitive to a MEK 1 specific inhibitor PD 98059 and was important for p97Eps8 mediated transformation , since transfection of vectors expressing dominant negative MEK 1 and p97Eps8 abrogated focus formation by p97Eps8 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , pretreatment of the cells with PD 98059 or SB 202190 or stable expression of a dominant negative mutant of ERK 2 or p 38 kinase impaired resveratrol induced p 53 dependent transcriptional activity and apoptosis , whereas constitutively active MEK 1 increased the transcriptional activity of p 53 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 , an activator of ERK , was found in the outer layer of Hassall ' s corpuscles where p ERK was expressed . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 ERK map kinase pathway and the PI 3 kinase Akt pathway independently mediate anti apoptotic signals in HepG 2 liver cancer cells . ^^^ However , we found that the MEK 1 inhibitor PD 98059 , but not the p 38 kinase inhibitor SB 203580 , potently induced apoptosis of the liver cancer cells in the presence of serum , indicating that the MEK ERK signaling pathway is required for serum dependent survival of HepG 2 cells . ^^^ These data indicate that survival of HepG 2 liver cancer cells depends upon serum and that both the MEK 1 ERK and the PI 3 kinase Akt pathways are required for survival signaling to the nucleus . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of MAP kinase kinases 1 and 2 ( MEK 1 and MEK 2 , respectively ) , which are upstream from ERK , with the specific MEK inhibitor U 0126 blocked both cell proliferation and ERK activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of ERK with the MEK 1 , 2 inhibitor PD 98059 increased apoptosis 2 fold , whereas the inhibitor of p 38 , SB 202190 , decreased the level of apoptotic cells by approximately 40 % . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Complement induced ERK activation was blocked in cells treated with GF109203X or Go 6976 , two selective PKC inhibitors , as well as by treatment with PD 098059 , an inhibitor of MEK 1 , the ERK kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In IMS 32 cells , activation of the Ras extracellular signal regulated kinase ( ERK ) pathway by adenoviral vector mediated expression of dominant active MEK 1 did not alter a basal level of CNTF expression , whereas inhibition of the Ras ERK pathway by using adenoviral vectors resulted in a marked increase in CNTF expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Myosin light chain phosphorylated by protein kinase C and Erk 2 phosphorylated by MEK 1 were poor substrates for CaMKPase , while a synthetic phosphopeptide corresponding to the sequence around the phosphorylation site of the former was not dephosphorylated by CaMKPase but that of the latter was fairly good substrate . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor PD 98059 ( 50 microM ) abolished the PDGF stimulated phosphorylation of ERK MAPKs and caldesmon and reduced cell migration by 50 % . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Immunohistochemical evaluation showed that the expression of iNOS , NF kappa B , MAPKs ( MEK 1 , ERK 2 ) , phosphotyrosine protein and c fos was increased in the terminal bronchioles but protein kinase C ( PKC ) , MEKK 1 , c jun , p 38 and c myc showed no change . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The role of the Ras mitogen activated protein kinase kinase ( MEK ) extracellular signal regulated kinase ( ERK ) cascade in mediating TGF beta induced morphological and functional effects were studied by pretreatment with the MEK 1 inhibitor PD 98059 and by measuring ERK 2 activation using immune complex kinase assays . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here , we show that both HCV genotype 1a and 3 core proteins activate MEK 1 and Erk1 / 2 MAP kinases and that the costitutive expression of the HCV core results in a high basal activity of Raf 1 and MAP / kinase / kinase , as determined by endogenous Raf 1 in vitro kinase assay and immunodetection of hyperphosphorylated Erk 1 and Erk 2 even after a serum starvation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Three different inhibitors of MEK1 / 2 abolished PE induced activation of S6K2 whereas expression of constitutively active MEK 1 activated S6K2 , without affecting the p 38 mitogen activated protein kinase and JNK pathways , indicating that MEK / ERK signaling plays a key role in regulation of S6K2 by PE . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Further analyses disclosed that transfection of dominant negative forms of raf 1 , MEK 1 , ERK 1 , ERK 2 , or wild type MEKK 1 all increased cystatin A promoter activity in normal human keratinocytes , whereas wild type raf 1 , ERK 1 , ERK 2 , or dominant negative forms of MEKK 1 , MKK 7 , or JNK 1 suppressed the promoter activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
TPA dependent ERK phosphorylation was also blocked by the MEK 1 inhibitors PD 098059 or U 0126 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Presently , we found that glucose acutely activated PKC zeta / lambda in rat adipocytes and rat skeletal muscle preparations by a mechanism that was independent of phosphatidylinositol 3 kinase but , interestingly , dependent on the apparently sequential activation of the dantrolene sensitive , nonreceptor proline rich tyrosine kinase 2 ; components of the extracellular signal regulated kinase ( ERK ) pathway , including , GRB 2 , SOS , RAS , RAF , MEK 1 and ERK1 / 2 ; and , most interestingly , phospholipase D , thus yielding increases in phosphatidic acid , a known activator of PKC zeta / lambda . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In addition , the GADD 45 promoter is strongly activated following expression of JNK 1 ; Raf 1 , which is an upstream activator of the ERK pathway ; or MEK 1 , an upstream activator of both the ERK and the JNK pathways . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 a specific inhibitor of MAP or Erk kinase 1 ( MEK 1 ) , the upstream kinase that phosphorylates Erk1 / 2 abolishes angiogenin induced Erk phosphorylation and cell proliferation without affecting nuclear translocation of angiogenin . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activation of Raf 1 and MEK 1 was coincident with Erk activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Infection of cultured cardiomyocytes with an activated MEK 1 expressing adenovirus induced robust phosphorylation of serine 105 in GATA 4 , while a dominant negative MEK 1 expressing adenovirus blocked agonist induced phosphorylation of serine 105 , implicating extracellular signal regulated kinase ( ERK ) as a GATA 4 kinase . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activation of the upstream ERK kinase ( MEK 1 ) paralleled ERK activation except in sparse cells in which the synergistic effects of menadione and PDGF on ERK could not be fully accounted for by MEK 1 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of ERK by the mitogen activated protein kinase kinase ( MEK 1 ) inhibitor PD 98059 ( 10 microM ) abolished AP 1 activation and apoptosis induction with SNAP . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Overexpression of dominant negative MEK 1 or treatment with PD 98059 abolishes MST 4 induced ERK activity , whereas dominant negative Ras or c Raf 1 mutants failed to do so , indicating MST 4 activates MEK1 / ERK via a Ras / Raf 1 independent pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
IGF 1 stimulation was followed by a PI3K dependent phosphorylation of AKT and BAD and an MEK 1 dependent phosphorylation of extracellular signal regulated kinase ( ERK ) 1 and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 , a specific inhibitor of the ERK activating kinase MEK 1 , abolished the TNF alpha induced ERK phosphorylation and osteoclast survival , and in these responses the involvement of Grb 2 and ceramide was observed . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Naloxone effectively blocked these actions of morphine , whereas a selective MEK 1 inhibitor , PD 98059 , inhibited the morphine induced increase in the phosphorylation of ERK and CREB , and the expression of CGRP and SP . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The phosphorylation of MEK 1 by cdk 5 resulted in inhibition of MEK 1 catalytic activity and the phosphorylation of extracellular signal regulated kinase ( ERK ) 1 / 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Overexpression of MEK 1 , an ERK activator , down regulated the 11beta HSD 2 activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Osteoblast proliferation induced by all BPs diminished to control levels in the presence of U 0126 , a specific inhibitor of the upstream kinase MEK 1 responsible for ERK phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Induction of GRP 78 by chronic hypoxia was completely abolished by pretreatment with PD 98059 [ a specific inhibitor of mitogen activated protein / extracellular signal regulated kinase ( ERK ) kinase ( MEK 1 ) ] or by overexpression of a dominant negative MEK 1 mutant , demonstrating a direct involvement of ERK in the induction of transcription at the GRP 78 promoter under these conditions . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We find the following : 1 ) the N terminal folding domain of ERK 3 functions in phosphoryl transfer reactions with the C terminal folding domain of ERK 2 ; 2 ) the C terminal halves of ERK 2 and ERK3DeltaC are primarily responsible for their subcellular localization in resting cells ; and 3 ) the N terminal folding domain of ERK 2 is required for its activation in cells , its interaction with MEK 1 , and its accumulation in the nucleus . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
While investigating the upstream signaling pathways , we found that ERK1 / 2 MAPK is activated and translocates to the nucleus to activate Elk 1 , and inhibition of the activation of ERK by specific inhibitors of MEK 1 blocks the up regulation of VEGF by low pH . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Surprisingly , MEK 2 but not MEK 1 was the principal mediator of estradiol induced activation of ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 , a specific inhibitor of the ERK activating kinase MEK 1 , also abolished the effects of IL 1alpha on ERK activation and osteoclast survival . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We found that expression of a fusion protein consisting of the Gab 1 PH domain and an active SHP 2 ( Gab1PH SHP2DeltaN ) induced constitutive Mek 1 and Erk 2 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Further , we investigated the molecular mechanisms underlying the effects of HGF and IFN gamma in BEAS 2B cells and found that the MEK 1 inhibitor PD 98059 , but not the p 38 M associated protein kinase inhibitor SB 203580 , abrogates HGF induced ERK activation and proliferation in response to HGF and serum . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In this study , we demonstrated that migration of MCF 7 breast cancer cells and HT 1080 fibrosarcoma cells on serum coated surfaces is stimulated by agents that activate ERK , including uPA , epidermal growth factor , and constitutively active MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor PD 98059 prevented ERK activation but not p 53 stabilization . ^^^ Conversely , enforced activation of ERK by overexpression of MEK 1 / Q56P sensitized cells to DNA damage induced apoptosis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of MEK 1 , 2 / ERK mitogenic pathway by estrogen with antiproliferative properties in rat aortic smooth muscle cells . ^^^ In 0 . 5 % serum treated RASMCs , estrogen dramatically inhibited the activity of extracellular signal regulated kinases ( ERK ) followed by inhibition of MEK 1 , 2 activity in dose dependent manner without affecting the other mitogen activating protein kinases ( MAPKs ) , c jun N terminal kinases ( JNK ) and p 38 . ^^^ These results indicate that the antiproliferative effects of estrogen in RASMCs involved ERK inhibition followed by the inactivation of MEK 1 , 2 and downregulation of Elk 1 expression . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , dominant negative ERK 2 or the MEK 1 inhibitor , PD 98059 , had no effect on p 53 phosphorylation at serine 20 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PAK 1 primes MEK 1 for phosphorylation by Raf 1 kinase during cross cascade activation of the ERK pathway . ^^^ Our findings indicate that PAK 1 primes MEK 1 for activation by Raf 1 and imply another level of regulation in the ERK cascade . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Western blot and immunohistochemistry were used to measure the expression of MEK 1 , MEK 2 and ERK 1 , ERK 2 protein . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Co transfection of the MAO B promoter with dominant negative forms of Ras , Raf 1 , MEKK 1 , MEK 1 , MEK 3 , MEK 7 , ERK 2 , JNK 1 , and p38 / RK inhibit the PMA dependent activation of the MAO B promoter . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In rat extensor digitorum longus ( EDL ) muscles , ( a ) AMPK activator , 5 aminoimidazole 4 carboxamide 1 beta d riboside ( AICAR ) , activated PYK 2 , ERK and aPKCs ; ( b ) effects of AICAR on ERK and aPKCs were blocked by tyrosine kinase inhibitor , genistein , and MEK 1 inhibitor , PD 98059 ; and ( c ) effects of AICAR on aPKCs and 2 deoxyglucose ( 2 DOG ) uptake were inhibited by genistein , PD 98059 , and PLD inhibitor , 1 butanol . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : ERK activity , MEK 1 , and p 42 and p 44 ERK dual phosphorylation were undetectable in the expanding , greatly proliferating and self renewing HP . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Neomycin induced ERK activation was both dose and time dependent and was attenuated by inhibitors of phosphatidylinositol 3 kinase , phosphatidylinositol bisphosphate ( PIP ( 2 ) ) specific phospholipase C , and MEK 1 , but not of protein kinase C . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
While the MEK 1 [ mitogen activated protein kinase ( MAP kinase ) / extracellular signal related kinase ( ERK ) kinase 1 ] inhibitor PD 98059 [ ( 2 amino 3 ' methoxyphenyl ) oxanaphthalen 4 one ] had no effect on leptin stimulated phosphorylation of STAT 3 Tyr705 , it greatly attenuated leptin ' s effects on STAT 3 Ser727 phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To further investigate the effect of UV induced Erk 2 activation on the expression of junD mRNA , cDNA encoding mitogen activated protein kinase kinase ( MEK 1 ) was overexpressed in ML 1 cells . ^^^ In contrast , the suppression of Erk activation with PD 98059 , a specific inhibitor of MEK 1 , inhibited UV and TPA induced junD mRNA expression , UV induced increases in caspase 3 activities , and cell death . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Ark repression was blocked by the MEK / ERK pathway inhibitor , PD 98059 , and dominant negative MEK 1 but was unaffected by dominant negative Ras . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Application of the MEK 1 inhibitor PD 98059 blocked TGFbeta 1 effects on Erk but had no effect on Akt / PKB phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activated MAPK / ERK kinase ( MEK 1 ) induces transdifferentiation of pigmented epithelium into neural retina . ^^^ In the present study , we show that ectopic expression of a constitutively activated allele of MEK 1 , the immediate upstream activator of the MAPK ERK , in chicken embryonic retina in ovo , induces transdifferentiation of the RPE into a neural like epithelium that is correlated with a downregulation of Mitf expression in the presumptive RPE . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Rac PAK signaling stimulates extracellular signal regulated kinase ( ERK ) activation by regulating formation of MEK 1 ERK complexes . ^^^ Activated forms of Rac and Cdc 42 could enhance the association of wild type ERK 2 with MEK 1 but not with MEK 2 in serum starved adherent cells . ^^^ In detached cells placed in suspension , ERK 2 was complexed with MEK 2 but not with MEK 1 . ^^^ However , upon replating of cells onto a fibronectin matrix , there was a substantial induction of MEK 1 ERK2 association and ERK activation , both of which could be inhibited by dominant negative PAK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The expression and phosphorylation of downstream targets of the EGFR , mitogen activating kinase kinase ( MEK 1 and 2 ) and extracellular signal regulated kinases 1 and 2 ( ERK 1 and 2 ) were not significantly affected . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The amount of Smad 2 protein was increased by transfection with a constitutively active MEK 1 and reduced by co transfection with the ERK phosphatase , HVH 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We report here that CD 40 induced TRAF 3 dependent activation of mitogen activated protein kinase ( MAPK ) / extracellular signal regulated kinase ( ERK ) kinase 1 ( MEK 1 ) is involved in the upregulation of IL 4 driven germline C epsilon transcription in a human Burkitt ' s lymphoma B cell line , DG 75 . ^^^ Antisense oligodeoxynucleotide ( ODN ) for TRAF 3 inhibited CD 40 induced activation of MEK 1 ERK pathway by decreasing expression of TRAF 3 protein , but antisense ODNs for TRAF 2 , 5 , and 6 were ineffective . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We demonstrate that exposure of post confluent 3T3 L 1 preadipocytes to insulin , isobutylmethylxanthine ( MIX ) , dexamethasone ( DEX ) , and fetal bovine serum induces a rapid but transient activation of MEK 1 as indicated by extensive phosphorylation of ERK 1 and ERK 2 during the initial 2 h of adipogenesis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Activation of ERK1 / 2 was induced by insulin and was dependent on the activation of MEK 1 , the kinase upstream of ERK in this pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The flavone PD 098059 is known to bind to the inactive forms of MEK 1 ( MAPK / ERK Kinase ) thus preventing activation by upstream activators . 20 microM PD 098059 ( IC 50 = 5 microM ) inhibited MAP kinase stimulated by either glucose , GRP , OT , GIP or PMA . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Use of the MEK 1 inhibitor U 0126 and dominant negative MEK 1 further showed that MSK 1 activation and c jun induction require the ERK pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Incubation of cell lines with the MEK 1 inhibitors PD 98059 or UO 126 after , but not during , cisplatin treatment completely inhibited cisplatin induced activation of ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The scaffold protein MP 1 ( MEK 1 partner ) assembles a scaffold complex in the ERK cascade . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This effect of endothelin 1 on LIFalpha and Jak 1 was attenuated by the MEK 1 inhibitor , PD 98059 , implicating involvement of the ERK kinases . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Phosphorylation of Ser ( 212 ) did not interfere with activating phosphorylation of MEK 1 at Ser ( 218 ) / Ser ( 222 ) or with binding to ERK 2 substrate . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The inhibition of the ERK kinases 1 and 2 ( MEK 1 and 2 ) by PD 098059 ( 2 ' amino 3 ' methoxyflavone ) failed to block the effect of Ang 2 on the Na ( + ) K ( + ) ATPase activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In the present study , we show that peptides corresponding to the MAPK docking sites of MEK 1 , MEK 2 , Ste 7 , Elk 1 and MAPK phosphatase ( MKP ) 2 potently inhibit MEK 2 phosphorylation of ERK 2 , ERK 2 phosphorylation of Elk 1 , and MKP 1 dephosphorylation of ERK 2 . ^^^ The MAPK docking site of MEK 1 also potently stimulated ERK 2 mediated phosphorylation of a target site on the same peptide . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Addition of the MEK 1 inhibitor PD 98059 had no effect on amylin induced apoptosis , suggesting that ERK activation does not play a role in this apoptotic scenario . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Roles of endogenous TGF beta / BMPs and MEK1 / Erk signaling in the action of AG 041R were investigated using the neutralizing soluble receptors and the MEK 1 inhibitor . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 mutant , which activates ERK , markedly down regulated expression of the insulin receptor ( IR ) and its major substrates , IRS 1 and IRS 2 , mRNA and protein , and in turn reduced tyrosine phosphorylation of IR as well as IRS 1 and IRS 2 and their associated phosphatidyl inositol 3 kinase ( PI3K ) activity . ^^^ In the context of our earlier report showing down regulation of glucose transporter 4 by MEK 1 ERK and MKK6 / 3 p 38 , the present findings suggest that chronic activation of ERK , p 38 , or JNK can induce insulin resistance by affecting glucose transporter expression and insulin signaling , though via distinctly different mechanisms . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A similar association can be detected between the EGFR and MEK 1 in receptor overexpressing cells , suggesting that multiple components of the ERK signaling pathway may become trapped in complexes with the EGFR . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These results imply that baicalein inhibits the ERK / MAPK cascade , acting on the phosphorylation of MEK 1 by Raf 1 . . ^^^ Baicalein inhibited the phosphorylation of exogenous MEK 1 by Raf 1 under cell free conditions , while it did not change the phosphorylation of exogenous p 42 MAPK by MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
No modifications in the expression of non phosphorylated MEK 1 , ERK 2 and GSK 3alpha / beta , as revealed by immunohistochemistry , were seen in AGD , but sarkosyl insoluble fractions were particularly enriched in JNK 1 and alphaCaM kinase 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The focus of this pathway is the dramatic activation of an endomembrane associated MEK 1 without the corresponding activation of the MEK substrate ERK . ^^^ This is because of the uncoupling of MEK 1 activation from ERK activation . ^^^ The mechanism of this uncoupling involves the cyclin B Cdc 2 dependent proteolytic cleavage of the N terminal ERK binding domain of MEK 1 and the phosphorylation of Thr ( 286 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mitogen activated protein kinase ( MAPK ) / extracellular signal regulated kinase ( ERK ) kinase 1 ( MEK 1 ) / ERK1 / 2 inhibitor PD 98059 interfered with this activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Identification of MEK 1 as a novel target for the treatment of neuropathic pain . ( 1 ) In the present study we have attempted to identify changes in gene expression which are associated with neuropathic pain using subtractive suppression hybridization analysis of the lumbar spinal cord of animals suffering streptozocin induced diabetic neuropathy . ( 2 ) Using this approach , we found a significant up regulation of several key components of the extracellular signal regulated kinase ( ERK ) cascade . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Notably , protection from EGF induced degradation and inhibition of EGF induced intracellular redistribution afforded by GH were both prevented by a MEK 1 inhibitor , suggesting a role for GH induced ERK activation in regulating the trafficking itinerary of the EGF stimulated EGFR . ^^^ Finally , we observed augmentation of early aspects of EGF signaling ( EGF induced ERK 2 activation and EGF induced Cbl tyrosine phosphorylation ) by GH cotreatment ; the GH effect on EGF induced Cbl tyrosine phosphorylation was also prevented by MEK 1 inhibition . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Treatment with the MEK 1 inhibitor PD 098059 ( 50 microM ) abolished EGF induced ERK activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of the ERK and protein kinase C signaling pathways with the MEK 1 inhibitor , U 0126 , and protein kinase C inhibitor , GF 1092030x , respectively , and chelating intracellular free calcium with 1 , 2 bis ( 2 aminophenoyl ) ethane N , N , N ' , N ' tetraacetic acid AM , which also reduced ERK1 / 2 activation , significantly reduced H2O2 induced AA release in MC+ / + expressing either group IIa or 5 PLA2s . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Tropomyosin phosphorylation was also induced by expression of a constitutively activated form of MEK 1 ( MEK ( CA ) ) , which confirms that its phosphorylation resulted from the activation of ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Amitotically activated mitogen activated protein kinase 1 ( MEK 1 ) fragments the pericentriolar Golgi stacks in mammalian cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Cotransfection of dominant negative mutant of Ras , Raf , and MEK 1 decreased the ET 1 induced increase in ET 1 transcription , suggesting that the Ras Raf ERK pathway is required for ET 1 action . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
There is evidence indicating that the CD site and the substrate binding region defined here are also utilized for MEK 1 recognition , and indeed , we demonstrate that the binding of MKP 3 , Elk 1 , and MEK 1 to ERK 2 is mutually exclusive . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Transfection of a vector expressing constitutively active MEK 1 generated a constant , high level of phosphorylated ERK comparable to the peak serum induced level and fully restored TCDD suppression without a TCDD mediated effect on ERK phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
CONCLUSION : Taken together , our results suggest that IL 1beta stimulation of cells activates MMP 9 secretion by the activation of the dual signalling pathways , the PI3K Akt and MEK 1 Erk and constitutive activation of these pathways were sufficient to induce MMP 9 secretion . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Finally , the selectivity of the MKK 4 , MEK 1 , and MEK 2 D sites for JNK versus ERK was quantified . ^^^ The MEK 1 and MEK 2 D sites displayed a strong selectivity for their cognate MAPK ( ERK 2 ) versus a non cognate MAPK ( JNK ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In this study , we focused on one of the physiological activators of ERK , mitogen activated protein kinase ( MAPK ) / ERK kinase 1 ( MEK 1 ) . ^^^ The considerable overlap between MEK 1 and its downstream effector , phospho ERK , suggests both a functional and mechanistic link . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , increased activation of the ERK pathway by constitutively active MEK 1 stimulates tTG mRNA expression , biosynthesis , and surface expression of tTG , whereas MEK inhibitors or dominant negative MEK 1 exert an opposite effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Although we effectively blocked phosphorylation of MAPKs ERK 1 and ERK 2 using the selective MEK 1 inhibitor PD 98059 , no quantitative changes of mRNA or protein levels of claudin 1 , occludin and ZO 1 could be detected in all cell lines investigated . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The effects of KCl and forskolin were mediated via the protein kinase A ( PKA ) and the extracellular signal regulated kinase ( ERK ) signaling pathways , since addition of the ERK kinase ( MEK 1 ) inhibitor PD 98059 and the PKA inhibitor H 89 inhibits neurite outgrowth . ^^^ KCl depolarization and forskolin synergistically activate the ERK signaling pathway , but whereas KCl mediated ERK activation depends on both PKA and MEK 1 , forskolin activates ERK in a PKA independent manner . ^^^ Finally , we find that KCl depolarization and forskolin both induce nuclear ERK 2 translocation via a PKA and MEK 1 dependent pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
When IFNalpha induced apoptosis , a hyperactivation of MEK 1 and ERK signalling and a decrease of the hypusine containing form and , thus , of eIF 5A activity were recorded . ^^^ The latter effect was again antagonized by the addition of EGF to IFNalpha pretreated cells , probably through the activation of the EGF > ERK dependent pathway , since the addition of the specific MEK 1 inhibitor PD 098059 abrogated the recovery of intracellular hypusine content induced by EGF in IFNalpha pretreated cancer cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , the reversibility of these cellular events , as well as the relative role of both MEK isoforms ( MEK 1 and MEK 2 ) and both ERK isoforms ( ERK 1 and ERK 2 ) during these processes , has not yet been investigated . ^^^ We now report that loss of constitutively active MEK 1 ( caMEK 1 ) and , thus , loss of active ERK1 / 2 in C7caMEK1 cells is associated with increased MEK 2 protein expression , reexpression of ERK 1 protein , and epithelial redifferentiation of these cells . ^^^ In contrast , loss of active MEK 1 ERK1 / 2 results in increased MEK 2 protein expression and reexpression of ERK 1 protein , concomitant with the restoration of epithelial phenotype and the ability to form cystic structures . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 as a specific inhibitor of MAPK kinase 1 ( MEK 1 ) , an up stream kinase of ERK , SB 203580 as a specific inhibitor of p 38 MAPK and CEP 11004 as a specific inhibitor of JNK cascade were used to investigate the role of ERK , p 38 MAPK and JNK in catabolizing arachidonic acid cascade in BEC . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
After 24 h incubation of the AML blasts with high ERK activity using PD 98059 ( New England BioLabs , Beverly , MA , USA ) , a selective inhibitor of MEK 1 phosphorylation , at concentrations of 20 and 40 microM , we observed a strong decrease in the levels of ERK1 / 2 activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We show that , similar to the known human KSR , hKSR 2 co immunoprecipitates with many signaling components of the Ras / MAPK pathway , including Ras , Raf , MEK 1 , and ERK 1 / 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Among the KRAS2 / BRAF wild type carcinomas , no mutations within pathway members MEK 1 , MEK 2 , ERK 1 , ERK 2 , RAP1B , or BAD were found . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Meanwhile , the pretreatment of the MEK 1 inhibitor ( PD 98059 ) completely blocked the cobalt chloride induced ERK 1 / 2 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment with wortmannin or 2 ( 4 Morpholinyl ) 8 phenyl 4H 1 benzopyran 4 one ( LY 294002 ) , a PI 3 kinase inhibitor , suppressed angiotensin 2 induced phosphorylation , but a mitogen activated protein kinase ( MAPK ) / extracellular signal regulated kinases ( ERK ) kinase 1 ( MEK 1 ) inhibitor , 2 ' Amino 3 ' methoxyflavone ( PD 98059 ) , and a p 38 MAPK inhibitor , 4 ( 4 Fluorophenyl ) 2 ( 4 methylsulfinylphenyl ) 5 ( 4 pyridyl ) 1H imidazole ( SB 203580 ) , had no effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We report that ( 1 ) ERK1 / 2 and their upstream modulators Ras , p 85 ( PI 3K ) , Rac 1 , and MEK 1 are found in the brush border ; ( 2 ) brush border associated ERK1 / 2 are stimulated by EGF and feeding ; ( 3 ) immunoblotting of proteins phosphorylated on SP / K motif suggests the presence of ERK substrates in the brush border , one of which could be actin ; and ( 4 ) pharmacological inhibition of ERK alters microvilli architecture . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Either PD 98059 or U 0126 , selective inhibitors of MEK , or overexpression of a dominant negative MEK 1 inhibited interleukin 1beta induced ERK activation and the expression of iNOS and COX 2 but had essentially no effect on the expression of VCAM 1 and Mn SOD . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Using in vitro assays , we show that platelet derived growth factor alpha ( PDGFA ) enhances medulloblastoma migration and increases downstream MAP2K1 ( MEK 1 ) , MAP2K2 ( MEK 2 ) , MAPK 1 ( p 42 MAPK ) and MAPK 3 ( p 44 MAPK ) phosphorylation in a dose dependent manner . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of MEK 1 by its specific inhibitor , PD 98059 substantially inhibited Erk activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Signalling from Ras to AP 1 is through the Raf / MEK [ mitogen activated protein ( MAP ) kinase kinase ] / ERK ( extracellular signal regulated kinase ) MAP kinase pathway as sustained activation of Raf 1 or Mek 1 modifies AP 1 composition and activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Enforced expression of doxycycline inducible p 21 ( CIP 1 ) or constitutively active MEK 1 significantly diminished 17 AAG / SAHA mediated lethality , indicating that interference with ERK activation and p 21 ( CIP 1 ) induction play important functional roles in the lethal effects of this regimen . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
However , PD 98059 , a specific inhibitor of mitogen activated protein kinase kinase ( MEK 1 ) , inhibited ERK activation by TGF beta ( 1 ) , and consequently attenuated TGF beta ( 1 ) enhancement of its own mRNA expression in PSCs . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Deprived FD / ( delta ) Raf 1 : ER , but not FD / ( delta ) B Raf : ER cells , expressed activated forms of MEK 1 and ERK after beta estradiol or IL 3 stimulation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Cotransfection of the dominant negative mutant of Ras , Raf and MEK 1 ( ERK kinase ) attenuated the Ang 2 enhanced ET 1 promoter activity , suggesting that the Ras / Raf / ERK pathway is required for Ang 2 induced ET 1 gene expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Co transfection of dominant negative mutant of Ras , Raf and MEK 1 attenuated the Ang 2 increased ET 1 promoter activity , suggesting that the Ras Raf ERK pathway is required for Ang 2 induced ET 1 gene . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We found that the treatment with MAP / ERK kinase 1 ( MEK 1 ) inhibitors PD 98059 or PD 184352 produced a reduction of phosphorylated ERK1 / 2 ( p ERK1 / 2 ) levels in myeloma cells of more than 80 % and prevented the increase of p ERK1 / 2 induced by interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mek 1 and Erk 1 depletion also caused cell cycle arrest at G 2 , suggesting that these enzymes are required for the G2 / M transition , whereas the loss of Mek 2 or Erk 2 caused arrest at G1 . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In 500 microM H ( 2 ) O ( 2 ) stimulated rat aortic smooth muscle cells , raxofelast dramatically attenuated the activation of mitogen activating protein kinase ( MAPK ) / ERK kinase 1 , 2 ( MEK 1 , 2 ) and protein kinase C ( PKC ) without affecting Ras expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 ( MEK 1 ; ERK kinase inhibitor ) abolished all agonist mediated ERK1 / 2 phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We now show that in double positive thymocytes Vav 1 is required for TCR induced activation of the ERK 1 and ERK 2 kinases via a pathway involving the Ras GTPase , and B Raf , MEK 1 , and MEK 2 kinases . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NK ( 1 ) R mediated cell death was inhibited by a dominant negative form of arrestin 2 , Raf 1 , or Nur 77 , by MEK1 / 2 specific inhibitors , and by RNA interference directed against ERK 2 or MEK 2 but not ERK 1 or MEK 1 and against Nur 77 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mitogen activated protein kinase extracellular signal related kinase ( ERK ) kinase 1 ( MEK 1 ) / ERK signaling has been implicated in the regulation of tumor cell invasion and metastasis . ^^^ Expression of a constitutively active form of MEK 1 promoted MMP 2 processing concomitant with the increase of MT 1 MMP levels , suggesting that MT 1 MMP is regulated by MEK / ERK signaling . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Both EGF receptor kinase inhibitor AG 1478 and MEK 1 inhibitor PD 98059 attenuated ERK activation and DNA synthesis enhanced by Ang 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
It was previously shown that Rac p 21 activated kinase ( PAK ) signaling regulated the physical association of MEK 1 with ERK 2 through phosphorylation sites in the proline rich sequence ( PRS ) of MEK 1 . ^^^ It was also shown that activation of MEK 1 and ERK by integrins depends on PAK phosphorylation of S 298 in the PRS . ^^^ Here we report a novel MEK 1 specific regulatory feedback mechanism that provides a means by which activated ERK can terminate continued PAK phosphorylation of MEK 1 . ^^^ Activated ERK can phosphorylate T 292 in the PRS , and this blocks the ability of PAK to phosphorylate S 298 and of Rac PAK signaling to enhance MEK 1 ERK complex formation . ^^^ Preventing ERK feedback phosphorylation on T 292 during cellular adhesion prolonged phosphorylation of S 298 by PAK and phosphorylation of S 218 and S 222 , the MEK 1 activating sites . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To determine the function of neuronal ERK activity in learning , a new line of transgenic mice was generated wherein dominant negative MEK 1 , the upstream obligate activator of ERK1 / 2 , was expressed by using a neuronal specific and pan neuronal Talpha 1 alpha tubulin promoter element . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Conditional expression of a dominant negative form of MEK 1 in the postnatal murine forebrain inhibited ERK activation and caused selective deficits in hippocampal memory retention and the translation dependent , transcription independent phase of hippocampal L LTP . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pull down assays showed that Plk 3 physically interacted with MEK 1 and ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inactivation of ERK accelerates erythroid differentiation of K 562 cells induced by herbimycin A and STI 571 while activation of MEK 1 interferes with it . ^^^ We studied the role of the mitogen activated protein kinase ( MAPK ) kinase 1 ( MEK 1 ) / extracellular signal regulated kinase ( ERK ) pathway during the erythroid differentiation of K 562 cells induced by tyrosine kinase inhibitors ( herbimycin A or STI 571 ) , using genetically modified cells ( constitutively MEK 1 activated K 562 : K562 / MEK1 , and inducible ERK inactivated K 562 : K562 / CL100 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Dendritic growth was also increased in cells transfected with dominant negative mutants of MEK 1 and ERK 2 but not with dominant negative mutants of MEK 5 and ERK 5 , suggesting that ERK1 / 2 is the primary mediator of this effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These effects were also MEK 1 dependent and suggested ERK pathway dependent influence of GH on EGF induced EGFR postendocytic trafficking and signaling . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The role of ERK and JNK in this process was studied by using adenoviruses that contain either a constitutively active mitogen activated protein kinase kinase 1 ( MEK 1 ) or a dominant negative JNK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Leukocyte specific protein 1 targets the ERK / MAP kinase scaffold protein KSR and MEK 1 and ERK 2 to the actin cytoskeleton . ^^^ We show that LSP 1 associates with the ERK scaffold protein KSR and with MEK 1 and ERK 2 . ^^^ Confocal microscopy showed that LSP 1 targets KSR , MEK 1 and ERK 2 to peripheral actin filaments . ^^^ Thus our data show that LSP 1 is a cytoskeletal targeting protein for the ERK / MAP kinase pathway and support a model in which MEK 1 and ERK 2 are organized in a cytoskeletal signaling complex together with KSR , PKCbetaI and LSP 1 and are activated by anti IgM in a PKCbetaI dependent manner . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 ERK 2 signaling pathway protects myocardium from ischemic injury in vivo . ^^^ METHODS AND RESULTS : To establish a causal relationship between ERK1 / 2 signaling and cardioprotection , we analyzed Erk 1 nullizygous gene targeted mice , Erk 2 heterozygous gene targeted mice , and transgenic mice with activated MEK 1 ERK1 / 2 signaling in the heart . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Our studies identified the Raf > MEK 1 > ERK 1 / 2 kinase module as the common signaling pathway mediating gastrin dependent ECL cell gene transcription . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitors also induced necrosis of other glucose deprived cell types including primary neurons at the same concentrations required for inhibition of ERK phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here we show that IKK serves as an essential component of a signaling pathway that involves activation of the Tpl 2 kinase and its downstream targets , MEK 1 and ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Interestingly , a transiently transfected dominant negative MEK 1 completely abrogated activation of a coexpressed green fluorescent protein ERK 2 reporter by all three of the factors . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In cells transfected with a catalytically defective mutant of MEK 1 , the upstream ERK specific kinase , both dopamine and SKF R 38393 mediated cytotoxicity was markedly attenuated , confirming the participation of the ERK signaling pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Both inhibition of the Ras Raf MEK ( mitogen activated protein / ERK kinase ) ERK cascade by either stable expression of dominant negative H Ras ( N 17 ) or addition of the MEK 1 inhibitor PD 98059 as well as inhibition of the phosphatidylinositol 3 kinase pathway by LY 294002 prevented GDNF induced migration and invasion of PANC 1 cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Additionally , Cdk5 / p35 phosphorylates MEK 1 and inhibits its ability to phosphorylate its downstream target Erk 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Prolonged activation of p 38 ( MAPK ) is accompanied by a rapid and sustained phosphorylation of Ras and ERK ; inhibition of ERK phosphorylation using the MEK 1 inhibitor PD 98059 rescued approximately 30 % of cells from bFGF induced death suggesting ERK plays a secondary role in the induction of death . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To test directly for a role of ERK in experience dependent zenk gene regulation , we infused an inhibitor of mitogen activated and extracellular regulated protein kinase kinase ( MEK 1 ; the enzyme responsible for ERK activation ) unilaterally into one auditory lobule just before song stimulation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MAPK activator MEK 1 is required for mitotic activation of p 42 MAPK in Xenopus egg extracts ; however , the identity of the kinase that activates MEK 1 is unknown . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The highly homologous kinases , Mek 1 and Mek 2 , act downstream of Ras and Raf to activate extracellular signal regulated kinase ( ERK ) mitogen activated protein kinases . ^^^ Both Mek 1 and Mek 2 triggered ERK phosphorylation . ^^^ Furthermore , a kinase dead Mek 1 mutant incapable of phosphorylating ERK proteins retained ability to mediate Mek 1 driven epidermal proliferation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of MEK 1 significantly inhibited extracellularly regulated kinase ( ERK ) 1 / 2 activation and insulin regulated Gene 33 transcription and protein levels in H4IIE cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Indeed , down regulation of the Raf / MEK / ERK pathway potentiates statin induced apoptosis because exposure to the MEK 1 inhibitor PD 98059 sensitizes AML cells to low , physiologically achievable concentrations of lovastatin . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The role of the extracellular signal regulated kinase ( ERK ) 1 and ERK 2 in the neutrophil chemotactic response remains to be identified since a previously used specific inhibitor of MEK 1 and MEK 2 , PD 98059 , that was used to provide evidence for a role of ERK 1 and ERK 2 in regulating chemotaxis , has recently been reported to also inhibit MEK 5 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The involvement of the Ras > Erk pathway in the protection of tumor cells from the apoptosis induced by IFNalpha is further demonstrated by both Ras inactivation by RASN 17 transfection and mitogen extracellular signal regulated kinase 1 ( Mek 1 ) inhibition by exposure to PD 098059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , in AI LNCaP HP cells , the Src MEK 1 / 2 ERK 1 / 2 CREB pathway was constitutively active . ^^^ Additional experiments showed that Src and the scaffold protein MNAR coimmunoprecipitated with AR , indicating a role for Src as an apical molecule in the Src MEK 1 / 2 ERK 1 / 2 CREB pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of ERK activation by the MEK 1 inhibitor PD 98059 was associated with a reduction of CTGF promoted alpha SMA protein expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We examined the effect of selective MAPK inhibitors , SB 239063 ( a p 38 MAPK inhibitor ) , U 0126 ( a mitogen activated and extracellular regulated kinase kinase , MEK 1 , inhibitor which inhibits downstream extracellular regulated kinase , ERK , activity ) , and SP 600125 ( a c jun N terminal kinase , JNK , inhibitor ) on IL 1beta induced proliferation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of MEK 1 inhibited basal as well as EGF induced ERK activation and migration . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Disruption of protein protein interactions by treatment with high salt was required to facilitate immunoprecipitation of active ERK 1 and co precipitation of MEK 1 . ^^^ The large protein complex containing ERK 1 and MEK 1 was resolved by velocity sedimentation from fragments of microtubules ; however , it did not contain other scaffolding components known to bind ERK and MEK . ^^^ We conclude that there are two independent nerve growth factor regulated ' signalling particles ' with an estimated size of 60 75 S , one containing ERK 1 and MEK 1 and the other containing B Raf . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To probe the mechanisms regulating the subcellular localization of ERK 2 , we used live cell imaging to examine the interaction between MEK 1 and ERK 2 . ^^^ Fluorescence resonance energy transfer ( FRET ) studies show that MEK 1 and ERK 2 directly interact and demonstrate that this interaction in the cytoplasm is largely responsible for cytoplasmic retention of ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To gain insight into the interactions of MP 1 with the ERK pathway , we analyzed the ability of MP 1 to bind to MEK 1 , ERK 1 , and to itself , and the regulation of these interactions . ^^^ An MP 1 mutant that lost MEK 1 binding no longer enhanced RasV 12 stimulated ERK 1 activity , and functioned as a dominant negative , consistent with the concept that MP 1 function depends on facilitating these oligomerizations . ^^^ These results support the concept that MP 1 functions as a regulator of MAP kinase signaling by binding to MEK 1 and regulating its association with a larger signaling complex that may sequentially service multiple molecules of ERK . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , we found that melanoma cell integrin alphav was required for MAPK kinase ( MEK ) 1 and extracellular signal regulated kinase ( ERK ) 1 / 2 activity in 3D collagen , whereas inhibition of MEK 1 activity induced apoptosis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Erk kinase is linked to Ah receptor expression , as demonstrated by reductions in total Ah receptor levels after overexpression of constitutively active MEK 1 . ^^^ In addition , Erk kinase activity modulates the transcriptional response because MEK 1 overexpression enhances TCDD initiated transactivation potential of the receptor . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The involvement of the Ras > Erk pathway in the protection of tumour cells from the apoptosis induced by IFNalpha is further demonstrated by both Ras inactivation by RASN 17 transfection and mitogen extracellular signal regulated kinase 1 ( Mek 1 ) inhibition by exposure to PD 098059 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MEK 1 inhibitor PD 98059 which blocks ERK activation had only a small inhibitory effect on DON induced apoptosis while the JNK inhibitor SP 600125 was without effect . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MIP 2 activated extracellular signal regulated kinase ( ERK ) 1 / 2 and Akt and both the mitogen activated protein kinase kinase 1 ( MEK 1 ) and phosphatidylinositol 3 kinase ( PI3K ) inhibitors 2 ' amino 3 ' methoxyflavone ( PD 98059 ) and wortmannin reduced the neuroprotective effect of MIP 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Surprisingly , dominant negative MEK 1 or Erk 2 displays only marginal inhibitory effect on LPA induced uPA up regulation , suggesting that a signaling pathway distinct from Raf MEK1 / 2 Erk is the prominent pathway responsible for this process . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Expression of constitutively active MEK 1 that selectively phosphorylates ERK was sufficient for TGF beta ( 1 ) mRNA stabilization in Swiss 3T3 fibroblasts . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : Compared with normal tissues , the protein levels of ERK 1 ( integral optical density value 159 526+ / 65 760 vs 122 807+ / 65 515 , P = 0 . 001 ) , ERK 2 ( 168 471+ / 95 051 vs 120 469+ / 72 874 , P < 0 . 001 ) , ERK 3 ( 118 651+ / 71 513 vs 70 934+ / 68 058 , P < 0 . 001 ) , P 38 ( 104 776+ / 51 650 vs 82 930+ / 40 392 , P = 0 . 048 ) and MEK 1 ( 116 486+ / 45 725 vs 101 434+ / 49 387 , P = 0 . 027 ) were increased in gastric cancer tissues . ^^^ Protein levels of ERK 2 , ERK 3 and MEK 1 in metastatic lymph nodes were 2 7 folds higher than those in adjacent normal mucosa . ^^^ The immunohistochemistry demonstrated that ERK 1 , ERK 2 , ERK 3 , p 38 and MEK 1 proteins were mainly localized in cytoplasm . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MEK 1 , a member of the mitogen activated protein kinase ( MAPK ) cascade that directly activates extracellular signal regulated kinase ( ERK ) , induces cardiac hypertrophy in transgenic mice . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Inhibition of the upstream ERK 1 , 2 activator MEK 1 , 2 with U 0126 prevented IL 1beta stimulated iNOS expression , while the p38MAPK inhibitor SB 03580 potentiated iNOS expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We found that ERK activation could be completely abolished if RINm5F cells were incubated with both bpV ( phen ) and PD 98059 , a specific inhibitor of upstream ERK kinase MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The data also show that the MEK 1 ( mitogen activated protein kinase kinase 1 ) / ERK ( extracellular signal regulated kinase ) pathway is only involved in bFGF induced transactivity of HIF 1 , but not HIF 1alpha expression , indicating roles for both the PI 3K / Akt and the MEK1 / ERK pathways in bFGF activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Glucose deprivation caused the loss of ATP and consequently cell death in immunostimulated astroglia , which was significantly blocked by the treatment with the ERK kinase ( MEK 1 ) inhibitor , PD 98059 ( 10 40 microM ) , to inhibit the ERK1 / 2 pathways . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The adaptor protein p 14 is associated with the cytoplasmic face of late endosomes that is involved in cell surface receptor endocytosis and it also directly interacts with MP 1 , a scaffolding protein that binds the MAP kinase ERK 1 and its upstream kinase activator MEK 1 . ^^^ The interaction of p 14 with MP 1 recruits the latter to late endosomes and the endosomal localization of p14 / MP1 MEK 1 ERK1 scaffolding complex is required for signaling via ERK MAP kinase in an efficient and specific manner upon receptor stimulation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Conversely , the inhibition of Erk activity induced by 12 h exposure to 10 mM Mek 1 inhibitor U 0126 antagonized the effects induced by HSP 90 transfection on apoptosis caused by MW EMF . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Signaling distal to MEK 1 involved extracellular signal regulated kinase ( ERK ) because inhibition of MEK 1 suppressed the Ang 2 induced activation of ribosomal S 6 kinase ( RSK ) , a substrate of ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The biological significance of ERK mediated inhibition of STAT 3 ( ( Tyr 705 ) ) phosphorylation was further assessed by treating the cells with an inhibitor ( PD 98059 ) of mitogen activated protein kinase kinase ( MEK ) or by transfecting the cells with a vector that expresses constitutively active MEK 1 . ^^^ Expression of constitutively active MEK 1 caused an increase of ERK activity and inhibited STAT 3 ( ( Tyr 705 ) ) phosphorylation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
This SDF1alpha induced ERK activity was dose dependent and could be inhibited by pre treatment of the cells with either pertussis toxin , an inactivator of G protein coupled receptors , or PD 98059 , a MEK 1 inhibitor . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Adenoviral expression of a constitutively active form of mitogen activated protein kinase ( MAPK ) or extracellular signal regulated kinase ( ERK ) kinase 1 ( MEK 1 ) was used to induce PKC independent p90RSK activation and downstream phosphorylation of eEF2K . 5 eEF2K phosphorylation was abolished by U 0126 ( 1 microM ) , a selective inhibitor of MEK 1 , and was significantly reduced by GF109203X at > or =3 microM and by Ro 31 8220 at > or =1 microM . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Treatment of myoblasts with LIF induced phosphorylation of ERK , and the LIF induced inhibitory effect on myogenesis was blocked by pretreatment with U 0126 , a specific MEK inhibitor , and transient transfection with dominant negative ( DN ) MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Peroxynitrite elicited a concentration and time dependent activation of ERK , secondary to the upstream activation of MEK 1 ( ERK kinase ) . ^^^ Thus , peroxynitrite activates ERK in cardiomyocytes through an unusual signaling cascade involving Raf 1 and MEK 1 , independently from EGFR and P 21 ( Ras ) , and also acts as a potent activator of JNK and p 38 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Upregulation of Egr 1 protein was inhibited by the extracellular regulated kinase ( ERK ) 1 / 2 inhibitor PD 98059 and overexpression of dominant negative MEK 1 downregulated Egr 1 luciferase reporter gene activity . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In the current study , we tried to develop a high throughput assay for LF activity based on native substrate , mitogen activated ERK kinase 1 ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Addition of PD 98059 , a MEK 1 inhibitor , suppressed ERK activation and significantly but incompletely reversed these signaling events and apoptosis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PD 98059 , a specific inhibitor of MEK 1 , almost completely blocked 2 AG induced ERK phosphorylation and AP 1 activation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In contrast , ERK activation in cells expressing active MEK 1 was not inhibited during mitosis or affected by roscovitine . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Further , using MEK 1 inhibitor PD 98059 we showed that leptin activates cPLA 2 through ERK induction . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , we show that DYRK1A prolongs the kinetics of ERK activation by interacting with Ras , B Raf , and MEK 1 to facilitate the formation of a Ras / B Raf / MEK1 multiprotein complex . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , transfection of an active mutant of MEK 1 ( ERK 1 / 2 kinase ) markedly increased superoxide production in rat aortic smooth muscle cells , as detected by dihydroethydium staining , suggesting that ERK 1 / 2 activation stimulates ROS generation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Our data further show that MP 1 , p 14 , and MEK 1 serve to inhibit Rho / Rho kinase functions necessary for the turnover of adhesion structures and cell spreading and reveal a signal channeling function for a MEK1 / ERK scaffold in orchestrating cytoskeletal rearrangements important for cell motility . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Estrogen induced cell cycle progression was not sensitive to the inhibition of ERK regulating kinases MEK 1 and 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment of HDFs with EPA inhibited UV induced MMP 1 expression in a dose dependent manner and also inhibited the UV induced activation of ERK and JNK by inhibiting ERK kinase ( MEK 1 ) and SAPK / ERK kinase 1 ( SEK 1 ) activation , respectively . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Here we found that the ability of constitutively active human MEK 1 and MEK 2 to stimulate ERK phosphorylation and to induce the neoplastic transformation of NIH 3T3 cells required the integrity of the D site . ^^^ ERK activation , cytoplasmic anchoring and release were completely retained in ' swap ' mutants in which MEK 2 ' s D site was replaced with the D site of MEK 1 or yeast Ste 7 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The purified pseudopodial fraction contains phosphotyrosinated proteins , including Met and FAK , and various signaling proteins , including Raf 1 , MEK 1 , ERK 2 , PKBalpha ( Akt 1 ) , GSK3alpha , GSK3beta , Rb , and Stat 3 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Gab 2 suppression reduces bFGF dependent activation of AKT but not ERK , and constitutively active AKT , but not constitutively active MEK 1 , reverses the hypersensitization . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Furthermore , whilst pre treatment of cells with either the JNK inhibitor SP 600125 , or the MEK 1 inhibitor PD 98059 , also reverses UV C and cisplatin induced apoptosis , the anti apoptotic effect of MKP 2 overexpression is not additive with SP 600125 but is with PD 098059 , suggesting that MKP 2 is involved in specifically terminating JNK activity and not ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
PGE ( 2 ) induced increases in neurotrophin 4 expression , Sp 1 transcriptional and DNA binding activity , Sp 1 mRNA and protein levels , and ERK phosphorylation were suppressed by antisense EP 3 oligodeoxynucleotide , inhibitors of phosphatidylinositol specific phospholipase C , conventional protein kinase C , and mitogen activated protein kinase / ERK kinase 1 ( MEK 1 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Dictyostelium mek 1 ( ) ( MEK 1 null ) and erk 1 ( ) cells exhibit severe defects in cell polarization and directional movement , but the molecules responsible for the mek 1 ( ) and erk 1 ( ) chemotaxis defects are unknown . ^^^ Microarray analysis reveals that some genes are precociously expressed in mek 1 ( ) and erk 1 ( ) cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Conversely , silencing of the B family regulatory subunits Balpha and Bdelta led to hyperactivation of ERK stimulated by constitutively active MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The addition of ERK 2 enhanced by fourfold the in vitro interaction of MEK 2 with IQGAP 1 without altering binding of MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK 2 shows a restrictive and locally selective mechanism of recognition by its tyrosine phosphatase inactivators not shared by its activator MEK 1 . ^^^ We have analyzed the contribution of specific residues of ERK 2 in the physical and functional interaction with the ERK 2 phosphatase inactivators PTP SL and MKP 3 and with its activator MEK 1 . ^^^ Remarkably , the cytosolic retention of ERK 2 by its activator MEK 1 was not affected by any of the analyzed ERK 2 single amino acid substitutions . ^^^ A chimeric MEK 1 protein , containing the kinase interaction motif of PTP SL , bound tightly to ERK 2 through its docking groove and behaved as a gain of function MAP kinase kinase that hyperactivated ERK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Moreover , phosphorylated extracellular signal regulated kinase ( ERK ) 1 / 2 and mitogen activated protein kinase ERK ( MEK ) 1 were increased in the Smad 4 mutants , suggesting that an upregulation of MEK 1 ERK1 / 2 signaling as a consequence of deletion of Smad 4 underlies the impaired cardiac function . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
We report here that hydroxyurea ( HU ) activates Erk via MEK 1 , a process that is sensitized by a constitutively active MEK 1 ( MEK1Q56P ) and attenuated by a dominant negative MEK 1 ( MEK1K97M ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A homogenous TR FRET based in vitro coupling assay for the MAP3Ks MEK 1 ERK2 kinase cascade was established and was used to screen for inhibitors of the ERK / MAPK pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Epo stimulated MCF 7 cell migration was blocked by the MEK inhibitor PD 098059 and by dominant negative MEK 1 , indicating an essential role for ERK . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
ERK phosphorylation and chemokine production in response to LPA require IL 4 dependent up regulation of MEK 1 expression by a pathway involving PI3K . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Overexpression of deltaCAPRI or a constitutive active form of Ras up regulated the expression level of matrix metalloproteinase 14 ( MMP 14 ) , which directly cleaves the ectodomain of RANKL , whereas Erk activation by expressing the constitutive active Mek 1 did not affect the MMP 14 expression or RANKL shedding . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The novel hybrid transcription factor TetR Elk 1 was regulated by MAPK ERK kinase 1 ( MEK 1 ) overexpression , and TetR CREB was regulated by protein kinase A ( PKA ) overexpression or elevation of cyclic AMP levels . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The DeltaRaf : ER cells were very sensitive to induction of apoptosis by the mitogen activated protein / ERK kinase ( MEK ) 1 inhibitor CI 1040 whereas parental cells were much less affected , demonstrating that the MEK 1 may be useful in eliminating Ras / Raf / MEK transformed cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Although Herceptin could down regulate both phosphatidylinositol 3 kinase ( PI3K ) / AKT signal and mitogen activated protein / extracellular signal related kinase ( ERK ) kinase 1 ( MEK 1 ) / ERK signal in HER 2 positive breast cancer cells , PI3K specific inhibitor but not MEK 1 specific inhibitor could decrease the survivin levels . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The authors have developed and run a high throughput screen to find inhibitors of V600E BRAF using an enzyme cascade assay in which oncogenic BRAF activates MEK 1 , which in turn activates ERK 2 , which then phosphorylates the transcription factor ELK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
IL 1beta activated ERK , and PD 98059 , a MEK 1 inhibitor , blocked IL 1beta enhancement of TGF beta ( 1 ) expression and secretion by PSCs . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The NH ( 2 ) terminal region of GLI 1 ( amino acids 1 130 ) is required for sensing the ERK pathway , as deletion of this domain produces active GLI 1 protein with greatly reduced response to activation by MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
No changes in the activity of B Raf were detectable during progesterone induced oocyte maturation , after egg fertilization , or during the early embryonic cell cycle , arguing against a role for B Raf in the mitotic activation of MEK 1 and p 42 MAPK . ^^^ Ras proteins can bring about activation of MEK 1 and p 42 MAPK in extracts , and Ras may contribute to signaling from the classical progesterone receptor during oocyte maturation and from receptor tyrosine kinases during early embryogenesis . ^^^ We found that both B Raf and C Raf , but not Mos , are required for Ras induced MEK 1 and p 42 MAPK activation . ^^^ These data indicate that two upstream stimuli , active Ras and active Cdc 2 , utilize different MAPKKKs to activate MEK 1 and p 42 MAPK . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
In response to LPS stimulation , Tpl 2 phosphorylates a downstream kinase , MEK 1 , leading to the activation of ERK signaling pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
To determine if PDGF stimulated w fb and n fb utilized the MAPK ERK pathway in a dependent manner , the upstream kinase MAPK kinase 1 ( MEK 1 ) was inhibited by PD 98059 . ^^^ In the presence of PDGF , fibroblasts with decreased growth rate express MAPK , and proliferation is further abrogated with addition of MEK 1 inhibitor , suggesting the importance of the MAPK ERK pathway regulating w fb and n fb proliferation . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Reduction of endogenous levels of betaarr or FLNA and a catalytically inactive dominant negative MEK 1 , which prevents ERK activation , inhibit membrane ruffle formation , indicating the functional requirement for betaarr , FLNA , and active ERK in this process . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Bcl G directly bound to ERKs and inhibited ERK activation by MEK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Cellular morphology , DNA fragmentation , nuclear condensation , total mitogen activated protein kinase / extracellular regulated protein kinase 1 ( ERK 1 ) , total ERK 1 protein , and phosphorylated ERK 1 protein products in cultured H9c2 myocardial cells were measured by actin immunofluorescence , agarose gel electrophoresis , nuclear condensation , and western blotting following stimulation with P . gingivalis spent growth medium or pre administration of U 0126 , a potent MEK 1 / 2 inhibitor . ^^^ Components of P . gingivalis spent culture medium not only resulted in increased total MEK 1 and ERK 1 protein products , but also caused increased cellular size , DNA fragmentation , and nuclear condensation in H9c2 cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Although Rac 1 deletion caused a significant reduction in phospho PAK 1 , AKT , and ERK under serum stimulation , reconstitution of active PAK 1 , but not AKT or MEK 1 , was able to rescue the actin cytoskeleton and adhesion phenotypes of the Rac 1 deficient cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The levels of MKK 6 , p 38 MAPK , MEK 1 and p 42 / 44 ERK were significantly higher in the vehicle treated SHRSP than in the WKY . ^^^ Furthermore , the levels of MEK 1 and p 42 / 44 ERK were significantly lower in the amlodipine than in the enalapril treated SHRSP group , whereas enalapril was more effective than amlodipine at increasing p Akt and endothelial NO synthase in SHRSP aortas , which were significantly lower in the vehicle SHRSP group than in the WKY group . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Pretreatment of HSFs with 2 ' , 4 ' , 7 THF inhibited UV induced MMP 1 expression in a dose dependent manner , and also inhibited the UV induced activations of ERK and JNK by inhibiting MEK 1 and SEK 1 activation , respectively . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Dominant negative MEK 1 , MEK 2 , and ERK 2 block PPARgamma induced EMT , whereas constitutively active MEK 1 and MEK 2 induce a mesenchymal phenotype similar to that evoked by PPARgamma . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
On the other hand , thapsigargin transiently enhanced phosphorylation status of mitogen activated protein kinase ( MAPK ) pathway , including extracellular signal regulated kinase ( Erk ) , p 38 MAPK and c JUN amino terminal kinase 1 ( JNK 1 ) simultaneously ; whereas specific inhibitors against MEK 1 and JNK only reduced the thapsigargin induced GDNF mRNA expression . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Specific MEK [ mitogen activated protein ( MAP ) kinase / extracellular signal regulated kinase ( ERK ) kinase ] inhibitors PD 98056 and UO 126 , as well as the use of a dominant negative form of MEK 1 abrogated STAT 3 Ser727 phosphorylation , suggesting involvement of MAP Kinase / Erk pathway . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
RESULTS : We have found that inhibition of ERK activation by the MEK inhibitor or dominant negative MEK 1 even immediately before the onset of S phase leads to the cessation of S phase entry . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Intracellular signaling for dermal collagen production was investigated using inhibitors of MEK 1 and by demonstration of ERK phosphorylation . ^^^ Adenosine A2A receptor ligation stimulated ERK phosphorylation , and A2A receptor mediated collagen production by dermal fibroblasts was blocked by MEK 1 inhibitors . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Taken together , our results strongly suggest that SHP 2 plays a critical role as a positive modulator for the production of TIMP 2 via MEK 1 Erk signaling in fibroblasts . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
A similar repression was also observed in cells that contained a dominant , nonactive form of ERK 2 but not in cells where ERK 1 phosphorylation was inhibited via overexpression of a dominant negative mutant MEK 1 protein . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Extracellular signal regulated kinase 2 ( ERK 2 ) was activated at low As ( 2 ) O ( 3 ) concentrations , and PD 98059 , an inhibitor of MEK 1 , blocked SH SY5Y cell differentiation by As ( 2 ) O ( 3 ) . ^^^ Extracellular signal regulated kinase 2 ( ERK 2 ) was activated at low As ( 2 ) O ( 3 ) concentrations , and PD 98059 , an inhibitor of MEK 1 , blocked SH SY5Y cell differentiation by As ( 2 ) O ( 3 ) . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MPM 2 phosphoepitope can be generated in vitro on bacterially expressed p42mapk by phosphorylation with either isoform of MAP kinase kinase ( MKK ) , MKK 1 , or MKK 2 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
MAP kinase ( ERK 2 ) and MAP kinase kinase ( MKK 1 ) activity , which are constitutively elevated in 5 mos and 5 raf transformed cells , exhibits levels in the F 1 revertant similar to those seen in nontransformed cells . ^^^ The results support the hypothesis that mos acts through the MAP kinase cascade ( MKK 1 and ERK 2 ) to induce cell transformation and that blocking 5 mos activation of that cascade ( possibly because of increased levels of phosphatase ) prevents transformation . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mitogen activated protein ( MAP ) kinase kinases ( MKKs ) are dual specificity protein kinases which activate p42mapk and p44mapk by phosphorylation of regulatory tyrosine and threonine residues . cDNAs for two isotypes of MKK , MKK 1 and MKK 2 , have been isolated from several species . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Transformation of immortalized rat embryo fibroblasts by a ts isolate of Moloney murine sarcoma virus ( Mo MuSVts 110 ) constitutively activates MAP kinases ( ERK 1 and ERK 2 ) and MAP kinase kinases ( MKK 1 and MKK 2 ) only at the permissive temperature when 5 mos kinase is present and active . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Both the acidic substitutions and the N terminal deletion increase Vmax , V / K ( m ) , ERK 2 , and V / K ( m ) , ATP , as is also observed following phosphorylation of wild type MKK 1 . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Induction of DNA synthesis and the activity of ERK and the ERK activating kinase MKK 1 were reduced only slightly after stimulation with PDGF BB in cells cultured on LN vs . those cultured on FN . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The mitogen activated protein ( MAP ) kinase pathway , which includes extracellular signal regulated protein kinases 1 and 2 ( ERK 1 , ERK 2 ) and MAP kinase kinases 1 and 2 ( MKK 1 , MKK 2 ) , is well known to be required for cell cycle progression from G 1 to S phase , but its role in somatic cell mitosis has not been clearly established . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Transfection with dominant negative mutants of Ras , Raf , and MKK 1 also inhibited the increase in ERK activity in response to calcium , as did treatment with herbimycin , a selective inhibitor for Src family kinases . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
Mutant MKK 1 , its inhibitors , and mutant ERK suppressed IFN gamma stimulated gene induction through the gamma IFN activated transcriptional element . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These data implicate the MKK 1 / ERK signalling cascade in Ca ( 2+ ) independent , histamine induced contraction of bovine trachealis . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The C terminal region of mitogen activated protein kinase kinase 1 and 2 ( MKK 1 and MKK 2 ) may function in regulating interactions with upstream kinases or the magnitude and duration of ERK mitogen activated protein kinase activity . ^^^ To further understand how phosphorylation at the C terminus of MKK 1 and protein interactions regulate MKK 1 function , we have generated several MKK 1 C terminal deletion mutants and examined their function in regulating MKK 1 localization , ERK protein activation , and cell growth . ^^^ ERK activation in response to constitutively active Raf 1 or growth factor stimulus was attenuated in cells expressing MKK 1 C terminal deletion mutants . ^^^ These findings identify a novel C terminal region between amino acid residues 330 and 379 on MKK 1 that is necessary for regulating the cytoplasmic distribution and subsequent ERK protein activation necessary for cell survival and viability . . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
The MKK 1 inhibitor , PD 98059 and transfection of a dominant negative MKK 1 blocked ras induced instability of MTs but did not modify the association of erk with MTs or affect MT stability of the parental cells . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
These include the large extracellular signal related kinase ( HUMERK ) , the ERK activator kinase ( PRKMK 1 ) , a new member of the RAS oncogene family , protein phosphatase 2 regulatory subunit B alpha isoform ( PPP2R2A ) , and a novel human gene with very high homology to a plant membrane transport family . ^^^
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q02750 and P28482	Pubmed	SVM Score :0.0
NA