| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.61982219 |
| We show that , consequently , B Raf interacts with MEK 1 and MEK 2 with a better affinity than does c Raf 1 , thus strengthening the notion that B Raf is a stronger MEK activator than c Raf l . 0.61982219^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| The prototype mitogen activated protein ( MAP ) kinase module is a three kinase cascade consisting of the MAP kinase , extracellular signal regulated protein kinase ( ERK ) 1 or ERK 2 , the MAP / ERK kinase ( MEK ) MEK 1 or MEK 2 , and the MEK kinase , Raf 1 or B Raf . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| In addition , this sequence was necessary for efficient activation of MEK 1 in vitro by B Raf but dispensable for activation by a novel MEK 1 activator which we have previously detected in fractionated fibroblast extracts . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Ha Ras containing point mutations at positions 65 and 71 possessed a twofold higher affinity for B raf and consequently MEK 1 . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| B raf gene encodes for multiple isoforms with Mek 1 kinase activity ] . ^^^ We also show that several B Raf isoforms interact with the Mek 1 protein ( MAP kinase kinase ) and phosphorylate this protein on serine residues 218 and 222 . . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| The first MEK activator has an apparent M ( r ) of 40 , 000 50 , 000 , was immunologically distinct from A Raf , B Raf , c Raf 1 , c MEKK , c Mos , MEK 1 , and MEK 2 , and was rapidly activated by serum , platelet derived growth factor ( PDGF ) , insulin , thrombin , and phorbol ester . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| B Raf protein isoforms interact with and phosphorylate Mek 1 on serine residues 218 and 222 . ^^^ Recent studies suggested that B Raf could be the intermediate molecule between Ras and Mek 1 ( MAP Kinase Kinase ) in signalling pathways specific of neural cells . ^^^ However , there has been no evidence for a direct interaction between B Raf and Mek 1 . ^^^ We report here that different B Raf isoforms can be co immunoprecipitated with anti Mek 1 antisera in COS 1 cells and that the kinase activity of B Raf is not required for its interaction with Mek 1 . ^^^ We also show that all B Raf isoforms tested phosphorylate Mek 1 in a time dependent manner , whereas kinase defective mutants fail to do so . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Regulation of the MAP kinase cascade in PC 12 cells : B Raf activates MEK 1 ( MAP kinase or ERK kinase ) and is inhibited by cAMP . ^^^ We show that B Raf is activated following NGF and PMA treatment of PC 12 cells , and that it can phosphorylate and activate MEK 1 . ^^^ However , cAMP inhibits B Raf autokinase activity as well as its ability to phosphorylate and activate MEK 1 . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| B Raf dependent regulation of the MEK 1 / mitogen activated protein kinase pathway in PC 12 cells and regulation by cyclic AMP . ^^^ In response to epidermal growth factor , nerve growth factor , and platelet derived growth factor , B Raf and Raf 1 are activated , phosphorylate recombinant kinase inactive MEK 1 , and activate wild type MEK 1 . ^^^ B Raf and Raf 1 are growth factor regulated Raf family members which regulate MEK 1 and MAP kinase activity in PC 12 cells . ^^^ The results demonstrate that tyrosine kinase receptor activation of MEK 1 and MAP kinase in PC 12 cells is regulated by B Raf and Raf 1 , whose activation is inhibited by protein kinase A , and MEK activators , whose activation is independent of cAMP regulation . . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| In contrast to Raf 1 immunodepleted lysates , preclearance of the cytosolic B Raf significantly reduced , by 96 % , the amount of MEK 1 activity associated with immobilized p21ras . ^^^ The decrease in MEK 1 activity correlated with complete loss in the binding of both B Raf and MEK 1 proteins with immobilized p21ras . ^^^ GMPPNP dependent activation of MEK 1 in brain extracts is dependent on the presence of the B Raf protein kinase . . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| B Raf and Raf 1 both efficiently phosphorylate MAP kinase kinase ( MEK 1 ) . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Receptor induced activation of other Ser / Thr kinases , such as c Raf , B Raf , Mek 1 , Mek 2 , Mekk , was not affected by wortmannin . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Moreover , the overexpression of MEK 1 ( 3A ) failed to block endogenous MEK 1 activation , although MEK 1 ( 3A ) formed complexes with both c Raf and B Raf as well as p42 / p44 MAPK . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In the yeast two hybrid interaction assay , mKsr 1 did not bind to either Ras , B Raf or Raf 1 , but interacted strongly with both MEK 1 and MEK 2 , activators of MAP kinase . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Wild type Galpha 12 overexpression prevented the decrease in MAPK activity and induced MEK 1 , but not B Raf , expression . ^^^ Moreover , the activated mutant of Galpha 12 induced an increase in MAPK activity and in the expression of both MEK 1 and B Raf . ^^^ These data indicate that the activated mutant of Galpha 12 stimulates the proliferation of 3T3 F442A preadipocytes , possibly through an increase in B Raf expression , independently of the MEK / MAPK pathway , but prevents differentiation , probably through an increase in MEK 1 expression and MAPK activity . . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| In vitro protein kinase assays demonstrated that activation of Raf 1 and B Raf resulted in the phosphorylation and activation of MAPK kinase ( MEK 1 ) , which subsequently phosphorylated p42mapk . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| A dominant negative mutant of B Raf , but not of Raf 1 , blocked the cAMP induced activation of ERK , indicating that B Raf is the MEK 1 upstream regulator mediating this cAMP effect . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Raf and MEK could abrogate the cytokine dependence of murine FDC PI and human TF 1 cells ; however , the frequency of transformation was dependent on the particular oncogene examined , as more factor independent cells were isolated after infection with activated retroviruses encoding A Raf or Raf 1 than were with MEK 1 or B Raf . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Down stream of PKA , the activity of B Raf was significantly decreased by nicotine in SH SY5Y cells , as determined by direct measurement of MEK 1 phosphorylation or in vitro kinase assays , whereas the modulation of MEK 1 phosphorylation by Raf 1 tended to increase . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Among the KRAS2 / BRAF wild type carcinomas , no mutations within pathway members MEK 1 , MEK 2 , ERK 1 , ERK 2 , RAP1B , or BAD were found . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Deprived FD / ( delta ) Raf 1 : ER , but not FD / ( delta ) B Raf : ER cells , expressed activated forms of MEK 1 and ERK after beta estradiol or IL 3 stimulation . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| We conclude that there are two independent nerve growth factor regulated ' signalling particles ' with an estimated size of 60 75 S , one containing ERK 1 and MEK 1 and the other containing B Raf . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| DYRK1A enhances the mitogen activated protein kinase cascade in PC 12 cells by forming a complex with Ras , B Raf , and MEK 1 . ^^^ Furthermore , we show that DYRK1A prolongs the kinetics of ERK activation by interacting with Ras , B Raf , and MEK 1 to facilitate the formation of a Ras / B Raf / MEK1 multiprotein complex . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| However , RIG 1 did not inhibit the transactivation induced by MEK 1 or MEK 3 , and failed to suppress the phosphorylation of extracellular signal regulated kinases 1 and 2 induced by the constitutively activated B Raf ( V599E ) . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| These compounds potently inhibit the activation of the unactivated human MEK 1 by upstream MAP3Ks ( including BRAF and COT ) , but do not inhibit the activity of the activated MEK 1 . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| The authors have developed and run a high throughput screen to find inhibitors of V600E BRAF using an enzyme cascade assay in which oncogenic BRAF activates MEK 1 , which in turn activates ERK 2 , which then phosphorylates the transcription factor ELK 1 . ^^^ These compounds did not inhibit a shortened activated MEK 1 to ELK 1 cascade but were active ( 0 . 5 27 . 9 microM ) in a V600E BRAF assay and represent a potential starting point for future drug discovery and development . . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| No changes in the activity of B Raf were detectable during progesterone induced oocyte maturation , after egg fertilization , or during the early embryonic cell cycle , arguing against a role for B Raf in the mitotic activation of MEK 1 and p 42 MAPK . ^^^ We found that both B Raf and C Raf , but not Mos , are required for Ras induced MEK 1 and p 42 MAPK activation . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Furthermore , three of five individuals without BRAF mutations had missense mutations in either MEK 1 or MEK 2 , downstream effectors of B Raf . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| Recently , we and others have generated mouse strains that allow the conditional ablation of the genes coding for Raf 1 , B Raf and MEK 1 . ^^^ |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and Q02750 |
Pubmed |
SVM Score :0.0 |
| NA |
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