| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Constitutively active MAPKK 1 fully activates ERK 2 and the transcription factor Elk 1 , but does not substitute for activated p21ras and synergize with calcium / calcineurin signals to induce NFAT . ^^^ Moreover , the combination of activated MAPKK 1 and Rac 1 could not substitute for activated p21ras and synergize with calcium signals to induce NFAT . ^^^ Thus , p21ras regulation of NFAT in T cells requires the activity of multiple effector pathways including those regulated by MAPKK 1 / ERK 2 and Rac 1 . . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Ha Ras containing point mutations at positions 65 and 71 possessed a twofold higher affinity for B raf and consequently MEK 1 . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Inasmuch as hSOS is involved in p21ras activation , we also demonstrated that mIgM ligation activated a Ras dependent kinase cascade in which sequential activation of Raf 1 and MEK 1 culminates in the activation of p 42 mitogen activated protein ( MAP ) kinase ( ERK 2 ) . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| The linkage between p21ras and MEK 1 was investigated to identify those elements which participate in the regulation of MEK 1 activity . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Association of MEK 1 with p21ras . ^^^ We have extended those observations to show that MEK 1 is the MEK activity previously described to associate with immobilized p21ras . ^^^ The association between MEK 1 and immobilized p21ras . ^^^ In contrast to Raf 1 immunodepleted lysates , preclearance of the cytosolic B Raf significantly reduced , by 96 % , the amount of MEK 1 activity associated with immobilized p21ras . ^^^ The decrease in MEK 1 activity correlated with complete loss in the binding of both B Raf and MEK 1 proteins with immobilized p21ras . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Recent studies have demonstrated the existence of a physical complex containing p21ras ( RAS ) , p74raf 1 ( RAF 1 ) , and MEK 1 . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| These results indicate that p21ras and MEK 1 are required for IFN gamma induced ERK 1 and ERK 2 activation . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Similarly , treatment of Ras inhibited myoblasts with the MEK 1 inhibitor PD 98059 revealed that elevated MAP kinase activity is not a significant contributor to the H Ras 12V effect . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| ERK 2 is activated via a kinase cascade initiated by activation of the G protein p21Ras followed by phosphorylation and activation of Raf 1 and mitogen activated protein kinase kinase 1 ( MEK 1 ) . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Conversely , the overexpression of constitutively active mutants of H ras or MEK 1 proteins had little or no effect on ChAT promoter activity , but strongly synergized with trapoxin . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| However , inhibition of mitogen activated protein kinase with PD 098059 did not preclude apoptosis and in fact increased the rate of apoptosis observed in serum deprived Ha ras transformed cells , indicating that the Ras / Raf 1 pathway induced apoptosis throughout a mitogen activated protein kinase kinase 1 ( MEK 1 ) independent pathway . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Activation of p44 / 42 ( MAPK ) induced by Hex A was inhibited by an 18 mer phosphorothioate derivatized antisense oligonucleotide ( 1 5 microM ) directed to human p 44 ( MAPK ) ; the mitogen activated protein kinase kinase ( MEK 1 ) inhibitor PD 98059 ( 5 microM ) ; the p 42 ( MAPK ) inhibitor Tyrphostin AG 126 ( 0 . 2 microM ) ; the farnesyl transferase inhibitors SCH 56582 ( 10 microM ) and FPT 3 ( 10 miroM ) , which inhibit p21Ras activation ; and Calphostin C ( 0 . 2 microM ) , an inhibitor of protein kinase C . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| In addition , dominant negative mutant of H Ras and a MAP kinase kinase ( MEK 1 ) inhibitor ( PD 98059 ) completely blocked ET 1 induced MAP kinase activation as well as [ 3H ] leucine and [ 3H ] thymidine uptake . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Stimulation of cells with epidermal growth factor ( EGF ) or fetal calf serum ( FCS ) resulted in activation of N Ras , but not K Ras , as well as activation of c Raf , MEK 1 , and p 42 MAPK . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| The effects of H RAS ( V 12 ) were mediated by activation of MAPK , as treatment with PD 98059 at concentrations verified to selectively inhibit MEK 1 reduced the frequency of prevalence of cells with micronuclei . ^^^ In addition , doxycycline inducible expression of a constitutively active MEK 1 , but not of a mutant RAC 1 , mimicked the effects of H RAS ( V 12 ) . ^^^ H RAS ( V 12 ) and activated MEK 1 also induced centrosome amplification , and chromosome misalignment . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| The H Ras ( v 12 ) effects on apoptosis were decreased by a mitogen activated protein kinase kinase ( MEK 1 ) inhibitor and recapitulated by doxycycline inducible expression of an activated MEK 1 mutant ( MEK 1 ( S217E / S221E ) ) . ^^^ Thus , coactivation of cAMP dependent pathways and ERK or JNK ( either through H Ras ( v 12 ) , Rac 1 ( v 12 ) , or MEK 1 ( S217E / S221E ) ) is inconsistent with cell survival . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| This conclusion is based on observations indicating that cyclin D 1 induction by transforming Ha Ras was depressed in a dose dependent manner by PD 98059 , a selective inhibitor of the mitogen activated kinase kinase MEK 1 , demonstrating that Ha Ras employs extracellular signal regulated kinases ( ERKs ) for signal transmission to the cyclin D 1 promoter . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Neither a dominant negative Ha Ras , constitutively activated Ha Ras , or a pharmacological inhibitor of MEK 1 affected T beta R 2 transcription . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Inhibitors of farnesyl protein transferase and MEK 1 , 2 induce apoptosis in fibroblasts transformed with farnesylated but not geranylgeranylated H Ras . ^^^ Treatment of H Ras CVLS transformed fibroblasts with MEK 1 , 2 inhibitors provides a pharmacological second signal to enhance FTI induced apoptosis . ^^^ The apoptotic response induced by MEK 1 , 2 inhibitors is much greater in magnitude in H Ras transformed cells than in K Ras transformed cells , also pointing to differences in pathway utilization and / or dependence for these two Ras isoforms . . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Accordingly , acute expression of H Ras ( V 12 ) or of a constitutively active MEK 1 also blocked TSH induced expression of Tg and NIS . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Whereas CaMK inhibition affected neither integrin stimulated Akt phosphorylation nor p21Ras or Mek 1 activity , it was necessary for Raf 1 activity . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Based on experiments with kinase inhibitors , with PCCL 3 cell lines with doxycycline inducible expression of RET / PTC mutants with substitutions of critical tyrosine residues in the kinase domain , and lines with inducible expression of activated mutants of H RAS and MEK 1 , RET / PTC was found to regulate mPGES 1 through Shc RAS MEK ERK . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Activation of the MEK 1 , 2 / ERK1 , 2 pathway either by pretreating cells with EGF or by expression of constitutively active K ras ( K rasV12G ) or MEK 1 ( MEK1 * ) increased the amplitude of G 17 stimulated Ca2+ release . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Moreover , the effects of oncogenic H ras expression on primary erythroid cells are blocked by the addition of U 0126 , a specific inhibitor of MEK 1 and MEK 2 , allowing normal terminal erythroid proliferation and differentiation . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| Both inhibition of the Ras Raf MEK ( mitogen activated protein / ERK kinase ) ERK cascade by either stable expression of dominant negative H Ras ( N 17 ) or addition of the MEK 1 inhibitor PD 98059 as well as inhibition of the phosphatidylinositol 3 kinase pathway by LY 294002 prevented GDNF induced migration and invasion of PANC 1 cells . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| We found that the MAPK pathway protein set consisting of the following gene products was present : FRS 2 alpha , GRB 2 , GAB 1 , SOS 1 , Ha ras , Raf1 / RafB , MEK 1 , 2 , 5 , MAPK / ERK1 , 2 , MAPK / ERK5 , and RSK 1 , 2 , 3 ( see abbreviations ) . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| To address this issue , we identified genes suppressed in HRAS transformed rat fibroblasts but upregulated after treatment with the demethylating agent 5 Aza 2 deoxycytidine and with the MEK 1 , 2 inhibitor U 0126 . ^^^ |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|