| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation of ERK1 / 2 but not that of stathmin was abolished by the MAPK / ERK kinase 1 inhibitor U 0126 , whereas both signaling pathways were blocked by the specific inhibitor of the epidermal growth factor receptor AG 1478 , suggesting the likely recruitment of this receptor upon prion clustering . . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Antibodies to estrogen receptor , epidermal growth factor receptor , c Fos , c Jun , and Ras proteins , protein kinases involved in receptor tyrosine kinase signal transduction pathway , MEK 1 and phosphotyrosine were utilized in immunocytochemical localization experiments to evaluate temporal expression of these factors in response to estrogen treatment . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| We find that co expression with caveolin 1 dramatically inhibits signaling from EGF R , Raf , MEK 1 and ERK 2 to the nucleus . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| In contrast , H ( 2 ) O ( 2 ) induced ERK activation is dependent on EGFR activation , which then leads to downstream Raf 1 and MEK 1 activation . . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Unlike wild type cells , FASMCF cells are highly sensitive to growth inhibition by an EgfR specific tyrosine kinase inhibitor ( TKI ) , ZD 1839 ( Iressa ) , and an inhibitor of the activation of MEK 1 ( MAPKK ) , PD 098059 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Both ouabain induced DNA synthesis ( BrdUrd uptake ) and MAPK42 / 44 phosphorylation were inhibited by the Src inhibitor PP 2 , the EGFR kinase inhibitor AG 1478 , the tyrosine kinase inhibitor genistein , and the MEK 1 inhibitor PD 98059 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| The expression and phosphorylation of downstream targets of the EGFR , mitogen activating kinase kinase ( MEK 1 and 2 ) and extracellular signal regulated kinases 1 and 2 ( ERK 1 and 2 ) were not significantly affected . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| A similar association can be detected between the EGFR and MEK 1 in receptor overexpressing cells , suggesting that multiple components of the ERK signaling pathway may become trapped in complexes with the EGFR . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| This GH induced EGFR phosphorylation was prevented by MEK 1 inhibitors but not by a protein kinase C inhibitor . ^^^ Notably , protection from EGF induced degradation and inhibition of EGF induced intracellular redistribution afforded by GH were both prevented by a MEK 1 inhibitor , suggesting a role for GH induced ERK activation in regulating the trafficking itinerary of the EGF stimulated EGFR . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Tyrphostins AG 1478 ( an epidermal growth factor receptor [ EGFr ] inhibitor ) and AG 490 ( a Janus kinase 2 [ JAK 2 ] inhibitor ) , SB 203580 ( a p 38 inhibitor ) , and PD 98059 ( a MEK 1 inhibitor ) were used . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| These effects were also MEK 1 dependent and suggested ERK pathway dependent influence of GH on EGF induced EGFR postendocytic trafficking and signaling . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| These compounds were competitive inhibitors of ATP binding by MEK 1 kinase , and they had minimal or no effect on Raf , epidermal growth factor receptor ( EGFR ) , Akt , cyclin dependent kinase 4 ( CDK 4 ) , or MK 2 kinases at concentrations > 100 fold higher than those that inhibited MEK 1 kinase . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| SP also induced MAPK mediated cell proliferation that was inhibited by TACE , matrix metalloproteinase ( MMP ) , EGFR , and MEK 1 inhibitors . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| The near infrared cytoblot was used to analyze phosphorylation of EGFR , Akt , Stat 3 , MEK 1 , and ERK1 / 2 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| STAT5b was tyrosine phosphorylated , in response to uPA , only in EGFR expressing cells . uPA induced cell proliferation was blocked by dominant negative MEK 1 , dominant negative STAT5b , and by expression of an EGFR that is mutated at Tyr 845 , which is essential for STAT5b activation . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Thus , peroxynitrite activates ERK in cardiomyocytes through an unusual signaling cascade involving Raf 1 and MEK 1 , independently from EGFR and P 21 ( Ras ) , and also acts as a potent activator of JNK and p 38 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| These assays were repeated in the presence of the Galphai inhibitor pertussis toxin ( PTx , 100 ng / mL ) , the Ras inhibitor manumycin A ( MA , 10 microM ) , the phosphatidyl inositol 3 ' kinase ( PI3K ) inhibitor wortmannin ( WN , 1 microM ) , the EGFR inhibitor AG 1478 ( AG , 10 nM ) , the MEK 1 inhibitor PD 98059 ( PD , 10 microM ) , the p 38 MAPK inhibitor SB 203580 ( SB , 10 microM ) , and the plasmin inhibitors aprotinin and epsilon aminocaproic acid . ^^^ Inhibition of EGFR reduced both ERK1 / 2 and p 38 MAPK activation . uPA activation of PI3K and MKK3 / 6 was EGFR dependent and that of MEK 1 was EGFR independent . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of cells incubated with imatinib demonstrated activation of EGFR and downstream signaling that was reduced by inhibition of mitogen activated protein / extracellular signal regulated kinase kinase 1 ( MEK 1 ) and EGFR , but not Her2 / ErbB2 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| After cellular stimulation with EGF and analysis at the level of AP 1 activation using a beta lactamase reporter gene , a 10 12 fold shift or 2 . 5 3 fold shift toward greater potency in the IC 50 was observed for EGFR and MEK 1 inhibitors , respectively , in the presence of RNAi targeting EGFR . ^^^ The combination of these two targeted agents was shown to increase the efficacy of EGFR and MEK 1 kinase inhibitors , leading to possible implications for overcoming or preventing drug resistance , lowering effective drug doses , and providing new strategies for interrogating cellular signalling pathways . . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| These results were confirmed through the use of EGFR antagonist AG 1478 and MEK 1 inhibitor PD 98059 . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| Sustained activation of MAPK3 / 1 beyond 3 h was independent of EGFR kinase activity but dependent on PKC activity , which appeared to form a self sustaining MAPK3 / 1 activation loop by activating RAF 1 , MAP2K1 , and PLA2G4 ( phospholipase A 2 [ all cytosolic isozymes ] ) . ^^^ |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02750 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|