| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.5781946 |
| We also show that EBF directly binds and activates the Pax 5 promoter . 0.5781946^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| All these genes including E2A and EBF are expressed in pro B cells lacking the transcription factor Pax 5 . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| EBF regulating Pax 5 transcription is enhanced by STAT 5 in the early stage of B cells . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In pre B cells mb 1 and CD 19 are regulate by EBF 1 in collaboration with Pax 5 and E proteins . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Here , we show that adult B cell development in IL 7 ( / ) and IL 7R alpha ( 2 / ) mice is arrested at the pre pro B cell stage due to insufficient expression of the B cell specific transcription factor EBF and its target genes , which form a transcription factor network in determining B lineage specification . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Recent studies have identified factors that regulate differentiation of hematopoietic stem cells into B cell progenitors ( PU . 1 and lkaros ) , and further differentiation of these progenitors into mature B cells ( NF kappa B , E2A , early B cell factor [ EBF ] and B cell specific activator protein [ BSAP ] ) . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| These data indicate that EBF plays an important role in the regulation of the Blk promoter in early B cell development and that EBF and BSAP are capable to act in cooperation to induce a target gene . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The present paper analyzes these components and the participation of some of them such as PU . 1 , Ikaros , Aiolos , GATA 3 , Egf 1 , E2A , EBF 1 , PAX 5 ( BSAP ) , TFE 3 , Oct 1 , Oct 2 , and Nf kappa B in the regulation of the differentiation stages of cells belonging to the B and T lymphoid lineages . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The transcription factors Pax5a or BSAP ( B cell lineage specific activator protein ) and EBF ( early B cell factor ) are constitutively expressed in spleen B cells and CD 40 cross linking induces increases in Pax5a and EBF binding activity compared with nonstimulated B cells . ^^^ CD 40 stimulation induces Pax5 / BSAP and EBF activation through a APE / Ref 1 dependent redox mechanism . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Both B 220 ( ) and B 220 ( + ) cells similarly express the IgH germ line transcript ( Imu ) , mRNAs for recombinase ( TdT , Rag 1 , and Rag 2 ) , and lymphoid specific transcription factors ( Pax 5 , EBF , E12 / E47 , Oct 2 , and Ikaros ) , but the DNA binding activity of Pax 5 , EBF , Oct 2 , and E2A are low in B 220 ( ) cells and while high in B 220 ( + ) cells . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| In the present report we show that prebursally committed B cell progenitors specifically express early B cell factor ( EBF ) and Pax 5 transcription factors . ^^^ Pax 5 and EBF are expressed in committed B cell progenitors prior to the colonization of the embryonic bursa of fabricius . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The three transcription factors E2A , EBF ( early B cell factor ) and Pax 5 play essential roles in B cell commitment checkpoints . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Significant progress has recently been made in our understanding of how transcription factors such as PU . 1 , Notch 1 , E2A , EBF , Pax 5 , Bcl 6 , Blimp 1 and XBP 1 control different developmental decisions during the onset and terminal phase of B lymphopoiesis . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| This inactivity correlates with either reduced levels or even a complete lack of several B cell specific transcription factors required for their expression : Oct 2 , OBF 1 , PU . 1 , E47 / E12 , PAX 5 and EBF . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Besides known regulator genes of early B cell development such as PAX 5 , E2A , and EBF , the most abundantly expressed genes in PBCs include ATM , PDGFRA , SIAH 1 , PIM 2 , C / EBPB , WNT 16 , and TCL 1 , the role of which has not been established yet in early B cell development . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Early B cell factor , E2A , and Pax 5 cooperate to activate the early B cell specific mb 1 promoter . ^^^ Previous studies have suggested that the early B cell specific mb 1 ( Igalpha ) promoter is regulated by EBF and Pax 5 . ^^^ In addition to EBF and Pax 5 binding sites , we detected occupancy of a consensus binding site for E2A proteins ( E box ) in pre B cells . ^^^ Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation , whereas binding sites for Pax 5 ( and its Ets ternary complex partners ) are required for promoter function in all mb 1 expressing cells . ^^^ Together , our studies demonstrate the complexity of factors regulating tissue specific transcription and support the concept that EBF , E2A , and Pax 5 cooperate to activate target genes in early B cell development . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| B lymphocyte development is regulated by the nuclear proteins Early B cell factor ( EBF ) and Pax 5 . ^^^ Roles of EBF and Pax 5 in B lineage commitment and development . ^^^ EBF and Pax 5 work separately and in concert to activate genes required for B cell differentiation . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Furthermore , E2A activity was inhibited in these cells as determined by decreased DNA binding and reduced expression of its target genes , including the transcription factors early B cell factor and Pax 5 . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Purified IL 7Ralpha+CD79a+CD19 cells transcribed the B lymphoid specific genes E2A , EBF , TdT , Rag 1 , had initiated DJH rearrangements , but almost lacked Pax 5 mRNA . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Phenotypic and expression studies show that Bcl11a functions upstream of the transcription factors Ebf 1 and Pax 5 in the B cell pathway . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Unlike pre proB cells from control animals , developmentally arrested IL 7R ( + ) B 220 ( + ) CD 19 ( ) NK1 . 1 ( ) Ly 6C ( ) cells failed to express EBF and Pax 5 . ^^^ These results suggest that transient downregulation of IL 7R signaling is a necessary event for induction of EBF and Pax 5 expression and B lymphocyte commitment . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The basic helix loop helix transcription factors encoded by the E2A gene function at the apex of a transcriptional hierarchy involving E2A , early B cell factor ( EBF ) , and Pax 5 , which is essential for B lymphopoiesis . ^^^ Herein , we demonstrate that the block in B lymphopoiesis imposed by the absence of E2A can be overcome by expression of EBF , but not Pax 5 , indicating that EBF is the essential target of E2A required for development of B lineage progenitors . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| In this context , early B cell factor ( EBF ) , a transcription factor required for B lymphopoiesis , potentiated activation of mb 1 by Pax 5 . ^^^ These data suggest a molecular basis for the hierarchical dependence of Pax 5 function on EBF and E2A in B lymphocyte development . . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The generation of B lymphocyte precursors is dependent on the combinatorial action of the transcription factors PU . 1 , Ikaros , E2A , EBF , and Pax 5 . ^^^ Loss of PU . 1 results in a severe reduction in Flk2+ , IL 7R+ lymphoid progenitors as well as impaired expression of EBF and Pax 5 . ^^^ Using EBF / mutant progenitors , we show that EBF induces Pax 5 and the early program of B lineage gene expression . ^^^ Importantly , Pax 5 does not rescue B cell development from either PU . 1 / or EBF / progenitors . ^^^ Pax 5 expression and function are contingent on EBF . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Furthermore , pDCs were present in the BM of RAG 1 ( / ) , CD127 / IL 7Ra ( / ) , and Pax 5 ( / ) mice . pDCs in RAG1 / GFP knock in mice could be subdivided , and immunoglobulin D ( H ) J ( H ) rearrangements , as well as transcripts for the B lineage related genes Pax 5 , mb1 / CD79a , ebf , and Bcl11a , were identified only in the green fluorescent protein positive ( GFP ( + ) ) pDC 1 subset . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Accordingly , CLPs from IL 7 deficient mice express lower amounts of early B cell factor ( EBF ) and Pax 5 than wild type CLPs , but similar amounts of GATA 3 . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| These components include the transcription factors PU . 1 , Ikaros , Bcl11a , E2A , EBF , and Pax 5 , as well as the cytokine receptors Flk 2 and IL 7R . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Through the use of novel biological network analyses , essential regulators of early B cell development , PAX 5 and EBF , were shown to be associated with a clear B lineage commitment in lymphoblastic t ( 11q23 ) / MLL leukemias . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| These components include the cytokine receptors Flk 2 and IL 7R as well as the transcription factors PU . 1 , Ikaros , Bcl11a , E2A , EBF , and Pax 5 . ^^^ Pax 5 expression and function are contingent on EBF . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| However , as early progenitors differentiate , it is increasing clear that a limited set of transcription factors ( e . g . ikaros , PU . 1 , E2A , EBF , pax 5 ) regulate B lineage specific genes , and that expression and stability of these factors is responsive to the microenvironment . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| How EBF mediates these effects has yet to be thoroughly explored , however , it initiates epigenetic modifications necessary for gene activation and the function of other transcriptional regulators , including Pax 5 . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| In this study , we found evidence for early B cell lineage commitment as determined by coexpression of CD 19 and CD45R ( B 220 ) when Pu . 1 expression was knocked down in HPC by specific small interfering RNA ( siRNA ) ; moreover , the expression of early B cell factor ( Ebf ) and paired box protein 5 ( Pax 5 ) transcription factors was induced when cells were treated by Pu . 1 siRNA , but not by control siRNA . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| Transcripts of the RAG 1 and RAG 2 genes and earlier defined transcription factors , such as E2A , early B cell factor , E 2 2 , PAX 5 , and IRF 4 , were specifically up regulated at stages undergoing Ig gene rearrangements . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| For example , forced activation of beta catenin quickly increased C / EBPalpha while reducing EBF and Pax 5 in lymphoid progenitors that then generated myeloid cells . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| In order to understand the mechanism underlying this defect we examined the expression of key transcription factors ( early B cell factor , EBF , and Pax 5 ) in this progenitor pool . ^^^ Here , we report that > 95 % of HSA ( lo ) CD 43 ( lo ) Lin ( ) cells express EBF and 5 % express Pax 5 . ^^^ |
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| Interacting proteins: Q02548 and Q9UH73 |
Pubmed |
SVM Score :0.0 |
| The reasons for this loss remained largely unknown as transcription factors with pleiotropic effects on B cell specific gene expression , namely E2A , EBF , and PAX 5 , are present in primary HRS cells . ^^^ |
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