| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.87233746 |
| XPC interacts with both HHR23B and HHR23A in vivo . 0.87233746^^^ Here , we show that XPC interacts in vivo , by means of the yeast two hybrid system , with both HHR23B and a second homologue of RAD 23 , HHR23A . 0.63551503^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.63662279 |
| The xeroderma pigmentosum group C ( XPC ) protein specifically involved in genome wide damage recognition for nucleotide excision repair ( NER ) was purified as a tight complex with HR23B , one of the two mammalian homologs of RAD 23 in budding yeast . 0.63662279^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Recently , we reported the cloning and analysis of three human NER genes , XPC , HHR23A , and HHR23B . ^^^ The XPC protein was found to be complexed with the product of HHR23B , one of the two human homologs of the Saccharomyces cerevisiae NER gene RAD 23 . ^^^ Interestingly , the HHR23B and XPC genes , the product of which forms a tight complex , were found to colocalize on band 3p25 . 1 . ^^^ Pulsed field gel electrophoresis revealed that the HHR23B and XPC genes possibly share a MluI restriction fragment of about 625 kb . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| The XPC protein is complexed with HHR23B , one of the two human homologs of the yeast NER protein , RAD 23 ( Masutani at al . ( 1994 ) EMBO J . 8 , 1831 1843 ) . ^^^ Using heparin chromatography , gel filtration and native gel electrophoresis we demonstrate that the majority of HHR23B is in a free , non complexed form , and that a minor fraction is tightly associated with XPC . ^^^ Gel filtration suggests the XPC HHR23B heterodimer resides in a high MW complex . ^^^ However , immunodepletion studies starting from repair competent Manley extracts fall to reveal a stable association of a significant fraction of the HHR 23 proteins or the XPC HHR23B complex with the basal transcription / repair factor TFIIH , or with the ERCC 1 repair complex . ^^^ Consistent with a function in repair or DNA / chromatin metabolism , immunofluorescence studies show all XPC , HHR23B and ( the free ) HHR23A to reside in the nucleus . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Because of the close chromosomal proximity of human XPC and HHR23B , the mouse XPC chromosomal location was determined ( 6D ) . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of XPC binding domain of hHR23B . hHR23B was originally isolated as a component of a protein complex that specifically complements nucleotide excision repair ( NER ) defects of xeroderma pigmentosum group C cell extracts in vitro and was identified as one of two human homologs of the Saccharomyces cerevisiae NER gene product Rad 23 . ^^^ Recombinant hHR23B has previously been shown to significantly stimulate the NER activity of recombinant human XPC protein ( rhXPC ) . ^^^ In this study we identify and functionally characterize the XPC binding domain of hHR23B protein . ^^^ Our work shows that binding to XPC alone is required and sufficient for the role of hHR23B in in vitro NER but does not rule out the possibility that the protein has additional functions in vivo . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Xeroderma pigmentosum complementation group C gene ( XPC ) encodes a protein of 125 kDa which is present in a tight complex with a 58 kDa protein encoded by the human homolog of the yeast RAD 23 gene , HHR23B ( Masutani , C . , Sugasawa , K . , Yanagisawa , J . , Sonoyama , T . , Ui , M . , Enomoto , T . , Takio , K . , Tanaka , K . , van der Spek , P . ^^^ The XPC HHR23B complex is required for excision of thymine dimers from DNA in a human excision nuclease system reconstituted from purified proteins . ^^^ In order to understand the role of the XPC HHR23B complex in excision repair , we have overexpressed each subunit alone and the heterodimer in heterologous systems , purified them , and characterized their biochemical properties . ^^^ Surprisingly , we find that the XPC subunit alone is sufficient for reconstitution of the human excision nuclease and that the HHR23B subunit has no detectable effect on the excision activity of the reconstituted system . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| HHR23B , a human Rad 23 homolog , stimulates XPC protein in nucleotide excision repair in vitro . ^^^ The complex consists of two tightly associated proteins : the XPC gene product and HHR23B , one of two human homologs of the Saccharomyces cerevisiae repair gene product Rad 23 ( Masutani et al . , EMBO J . 13 : 1831 1843 , 1994 ) . ^^^ The recombinant human XPC ( rhXPC ) protein exhibited a high level of affinity for single stranded DNA and corrected the repair defect in XP C whole cell extracts without extra addition of recombinant HHR23B ( rHHR23B ) protein . ^^^ To investigate the role of HHR23B , we fractionated the XP C cell extracts and constructed a reconstituted system in which neither endogenous XPC nor HHR23B proteins were present . ^^^ Stimulation of XPC by HHR23B was found with simian virus 40 minichromosomes as well as with naked plasmid DNA and with UV as well as N acetoxy 2 acetylfluorene induced DNA lesions , indicating a general role of HHR23B in XPC functioning in the genome overall nucleotide excision repair subpathway . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| XPC hHR23B protein complex is specifically involved in nucleotide excision repair ( NER ) of DNA lesions on transcriptionally inactive sequences as well as the nontranscribed strand of active genes . ^^^ Here we demonstrate that not only highly purified recombinant hHR23B ( rhHR23B ) but also a second human homolog of the Saccharomyces cerevisiae Rad 23 repair protein , hHR23A , stimulates the in vitro repair activity of recombinant human XPC ( rhXPC ) , revealing functional redundancy between these human Rad 23 homologs . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Although a small proportion of hHR23B is tightly complexed with the XP C responsible gene product , XPC protein , a vast majority exists as an XPC free form , indicating that hHR23B has additional functions other than NER in vivo . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Cells from humans with xeroderma pigmentosum group C do not perform NER in the bulk of the genome and are corrected by XPC protein , which forms a complex with hHR23B protein . ^^^ Recombinant XPC , hHR23B , and XPC hHR23B complex were purified . ^^^ In a reconstituted repair system , hHR23B stimulated XPC activity tenfold . ^^^ Electrophoretic mobility shift competition measurements revealed a 400 fold preference for binding of XPC hHR23B to UV damaged over non damaged DNA . ^^^ Binding of XPC hHR23B to UV damaged DNA was very fast . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Before the excision reaction , DNA damage is recognized by a complex originally thought to contain the XP group C responsible gene product ( XPC ) and the human homologue of Rad 23 B ( HR23B ) . ^^^ We demonstrate that nearly all XPC complexes contain CEN 2 , that CEN 2 interacts directly with XPC , and that CEN 2 , in cooperation with HR23B , stabilizes XPC , which stimulates XPC NER activity in vitro . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| XPA , XPC hHR23B , RPA , and TFIIH all are the damage recognition proteins essential for the early stage of nucleotide excision repair . ^^^ Instead , XPC hHR23B was effectively displaced from the damaged DNA by the combined action of RPA and XPA . ^^^ A mutant RPA lacking the XPA interaction domain failed to displace XPC hHR23B from damaged DNA , suggesting that XPA and RPA cooperate with each other to destabilize the XPC hHR23B damaged DNA complex . ^^^ Interestingly , the presence of hHR23B significantly increased RPA / XPA mediated displacement of XPC from damaged DNA , suggesting that hHR23B may modulate the binding of XPC to damaged DNA . ^^^ Together , our results suggest that damage recognition occurs in a multistep process such that XPC hHR23B initiates damage recognition , which was replaced by combined action of XPA and RPA . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| The NER earliest step is lesion recognition by a complex formed by XPC and HHR23B proteins . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Primary DNA damage sensing in mammalian global genome nucleotide excision repair ( GG NER ) is performed by the xeroderma pigmentosum group C ( XPC ) / HR23B protein complex . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Because the vast majority of XPC forms a complex with HR23B rather than HR23A , we investigated possible differences between the two Rad 23 homologs in terms of their effects on the XPC protein . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| The expression levels of XPC and hHR23B , two NER proteins , are correlated with PKCzeta expression . ^^^ Altogether , these results strongly suggest that PKCzeta could act as a modulator of NER activity by regulating the expression of XPC / hHR23B heterodimer . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| In mammals , the complex of HR23B ( Rad 23 homolog ) and XPC ( yeast Rad 4 homolog ) has been suggested to function in the damage recognition step of NER . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| We studied single nucleotide polymorphisms ( SNPs ) and their corresponding haplotypes in 6 genes ( ERCC 1 , ERCC2 / XPD , ERCC4 / XPF , ERCC5 / XPG , RAD23B and XPC ) involved in NER in a population based case control study of lung cancer in Xuan Wei . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Solution structure and backbone dynamics of the XPC binding domain of the human DNA repair protein hHR23B . ^^^ Human cells contain two homologs of the yeast RAD 23 protein , hHR23A and hHR23B , which participate in the DNA repair process . hHR23B houses a domain ( residues 277 332 , called XPCB ) that binds specifically and directly to the xeroderma pigmentosum group C protein ( XPC ) to initiate nucleotide excision repair ( NER ) . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| METHODS : Expression of CSA , CSB , XPC , hHR23B , XPA , XPB , ERCC 1 and p 53 genes was analyzed by quantitative RT PCR and immunoblotting in 26 HCC and 9 normal livers . ^^^ XPA , XPC , hHR23B and ERCC 1 mRNA levels were significantly increased ( p < 0 . 05 ) in HCC arising in cirrhotic livers compared to non fibrotic tissue . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| HHR23A and hHR23B are the human homologs of Saccharomyces cerevisiae Rad 23 . hHR23B is associated with the nucleotide excision repair ( NER ) factor xeroderma pigmentosum C ( XPC ) protein and is required for global genome repair . ^^^ Co immunoprecipitation experiments revealed that hHR23A was associated with a small portion of hHR23B and the majority of p 53 protein , indicating that hHR23A regulates the function of XPC by its association with the NER activator p53 . . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| No significant associations were observed for HR23B codon 249 , XPG codon 1104 , XPC codon 939 , XPF codon 415 , XPF nt 2063 , ERCC 6 codon 1213 or ERCC 6 codon 1230 . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Odds ratios ( ORs ) were calculated for breast cancer and smoking , and for breast cancer and nine non synonymous coding polymorphisms in six NER genes ( XPD codons 312 and 751 , RAD23B codon 249 , XPG codon 1104 , XPC codon 939 , XPF codons 415 and 662 , and ERCC 6 codons 1213 and 1230 ) . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| DNA was extracted from blood samples and 15 common nonsynonymous SNPs in seven nucleotide excision repair genes [ XPC , RAD23B ( hHR23B ) , CSB ( ERCC 6 ) , XPD ( ERCC 2 ) , CCNH , XPF ( ERCC 4 ) , and XPG ( ERCC 5 ) ] were genotyped . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| T [ CPD ] T / AG was inactive in HeLa extracts , or in extracts deficient in the photoproduct binding proteins DDB or XPC * hHR23B , arguing against interference from the nucleotide excision repair pathway . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Human centrin 2 is a component of the nucleotide excision repair system , as a subunit of the heterotrimer including xeroderma pigmentosum group C protein ( XPC ) and hHR23B . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| We evaluated the influence of common genetic variation in the NER pathway on bladder cancer risk by analyzing 22 single nucleotide polymorphisms ( SNP ) in seven NER genes ( XPC , RAD23B , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , and ERCC 6 ) . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| XP proteins are key players in several steps of the NER process , including DNA strand discrimination ( XPA , in complex with replication protein A ) , repair complex formation ( XPC , in complex with hHR23B ; XPF , in complex with ERCC 1 ) and repair factor recruitment ( transcription factor IIH , in complex with XPG ) . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| Centrins are highly conserved calcium binding proteins involved in the nucleotide excision repair pathway as a subunit of the heterotrimer including the XPC and hHR23B proteins . ^^^ |
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| Interacting proteins: Q01831 and P54727 |
Pubmed |
SVM Score :0.0 |
| NA |
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