| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.6800983 |
| Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair , without a preassembled repairosome . 0.6800983^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Whereas transfer of a wild type p 53 expression vector by microinjection or retroviral infection into primary normal human fibroblasts resulted in apoptosis , primary fibroblasts from individuals with xeroderma pigmentosum ( XP ) , who are deficient in DNA repair and have germ line mutations in the XPB or XPD gene , but not in the XPA or XPC gene , have a deficiency in the apoptotic response . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| However , immunodepletion studies starting from repair competent Manley extracts fall to reveal a stable association of a significant fraction of the HHR 23 proteins or the XPC HHR23B complex with the basal transcription / repair factor TFIIH , or with the ERCC 1 repair complex . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Furthermore , reconstitution of the human excision nuclease was observed with a mixture of five repair factors ( XPA , XPC , XPG , TFIIH , and RPA ) and the recombinant XPF ERCC 1 , thus verifying that no additional protein factors are needed for the specific dual incisions characteristic of human excision repair . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| To investigate sequence specific repair rate patterns in XP and CS cells , we conducted a similar analysis in XPA , XPB , XPC , XPD , and CSB fibroblasts . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Stable associations of the ERCC 1 complex with other known repair factors ( XPA , XPC , XPG and TFIIH complex ) could not be detected . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The incision reaction was then reconstituted with the purified proteins RPA , XPA , TFIIH ( containing XPB and XPD ) , XPC , UV DDB , XPG , partially purified ERCC1 / XPF complex , and a factor designated IF 7 . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| TFIIH is shown to complement three different cell extracts deficient in excision repair : XPB / ERCC3 , XPC and XPD / ERCC2 . ^^^ The complementation of XPB and XPD is a consequence of ERCC 3 and ERCC 2 being integral subunits of TFIIH , whereas complementation of XPC is due to an association of this polypeptide with TFIIH . ^^^ TFIIH is shown to complement three different cell extracts deficient in excision repair : XPB / ERCC3 , XPC and XPD / ERCC2 . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The dual incision reaction was reconstituted using the purified mammalian proteins XPA , RPA , XPC , TFIIH , XPG , and a fraction containing ERCC 1 XPF and IF 7 . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| We show that , while XPA binding to damaged DNA is ATP independent , stable association of TFIIH with DNA lesions is promoted by ATP hydrolysis and is dependent on the integrity of XPA and XPC proteins in the cell extract . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| In this study , the mRNA levels of the different genes involved in NER ( ERCC 1 , XPA , XPB , XPC , XPD , XPF ) were examined in a panel of eight different human cancer cell lines , together with the overall DNA repair capacity using a host cell reactivation assay of a damaged plasmid . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The dual incisions are accomplished by sequential and partly overlapping actions of six repair factors , RPA , XPA , XPC , TFIIH , XPG , and XPF . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| In mammalian cells the XPC hHR23B , XPA , RPA and TFIIH factors may all have a role in damage recognition . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| These data support a mechanism in which TFIIH associated helicase activity and XPC protein catalyze initial formation of the key open intermediate , with full extension to the cleavage sites promoted by the other core nucleotide excision repair factors . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| With the aim to devise a long term gene therapy protocol for skin cancers in individuals affected by the inherited autosomal recessive xeroderma pigmentosum we transferred the human DNA repair XPA , XPB / ERCC3 and XPC cDNAs , by using the recombinant retroviral vector LXSN , into primary and immortalized fibroblasts obtained from two XP A , one XP B ( associated with Cockayne ' s syndrome ) and two XP C patients . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| This interaction is thought to be mediated through the specific affinity of XPC for the TFIIH subunits XPB and / or p 62 , which are essential for both basal transcription and nucleotide excision repair . ^^^ Here we demonstrate that XPC HR23B interacts with general transcription factor IIH ( TFIIH ) both in vivo and in vitro . ^^^ Interestingly , association of TFIIH with DNA was observed in both wild type and XP A cell extracts but not in XP C cell extracts , and XPC HR23B could restore the association of TFIIH with DNA in XP C cell extracts . ^^^ Moreover , we found that XPC HR23B was necessary for efficient association of TFIIH with damaged DNA in cell free extracts . ^^^ We conclude that the XPC HR23B protein complex plays a crucial role in the recruitment of TFIIH to damaged DNA in global genome repair . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| We have used the same study persons to investigate the correlation between expression of eight genes involved in nucleotide excision repair and DNA repair capacity . mRNA levels of XPA , XPB , XPC , XPD , XPF , XPG , CSB and ERCC 1 in primary lymphocytes from 33 individuals were quantified by dot blots and normalized to beta actin . ^^^ ERCC 1 and XPD mRNA quantities were highly correlated ( r=0 . 89 ; P < 10 ( 11 ) ) while XPA , XPB , XPC , XPG , XPFand CSB mRNAs were moderately correlated ( r=0 . 2 0 . 7 ) . ^^^ In order to see if ERCC 1 or XPD activity was limiting for DNA repair , we cotransfected with plasmids encoding NER genes , thus over expressing either XPB , XPC , XPD , CSB or ERCC 1 in the host cell reactivation assay . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| We report here the different ways in which four subunits of the basal transcription / repair factor TFIIH ( XPB , XPD , p 62 and p 44 ) and the damage recognition XPC repair protein can enter the nucleus . ^^^ In agreement with the identification of more than one putative nuclear localization signal ( NLS ) in their protein sequences , XPB , XPC , p 62 and p 44 chimeras were rapidly sorted to the nucleus . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Loss of heterozygosity ( LOH ) analysis , using the microdissected tissues , for the XPA , XPB , XPC , XPD , XPE , XPF , XPG and the transcription coupled repair factor , Cockayne syndrome B ( CSB ) revealed that NER factors were abnormal in 30 . 0 % ( 3 / 10 cases ) of oral squamous cell carcinomas . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The damage recognition complex XPC hHR23B appears to be essential for the recruitment of all subsequent NER factors in the preincision complex , including transcription repair factor TFIIH . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Loss of heterozygosity ( LOH ) analysis for the XP , XPA , XPB , XPC , XPD , XPE , XPF , XPG and the transcription coupled repair factor , Cockayne syndrome B ( CSB ) revealed that NER factors were abnormal in 62 . 1 % of ovarian tumors ( 18 / 29 ) , 16 . 7 % of colon ( 2 / 12 ) and 22 . 2 % lung ( 2 / 9 ) carcinomas . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Human nucleotide excision repair is initiated by six repair factors ( XPA , RPA , XPC HR23B , TFIIH , XPF ERCC 1 , and XPG ) which sequentially assemble at sites of DNA damage and effect excision of damage containing oligonucleotides . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| XPA , XPC hHR23B , RPA , and TFIIH all are the damage recognition proteins essential for the early stage of nucleotide excision repair . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| These results suggest that following initial damage recognition , the carboxy terminus of XPC may be essential for the recruitment of TFIIH , and that most truncation mutations identified in XP C patients result in non functional proteins . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The total cellular levels of XPC and XPB were similar in both p 53 WT and Null cells and remained unchanged up to 24h following UV irradiation . ^^^ Tumor suppressor p 53 dependent recruitment of nucleotide excision repair factors XPC and TFIIH to DNA damage . ^^^ In this study , the regulatory effect of p 53 , on the spaciotemporal recruitment of XPC and TFIIH to DNA damage sites , was investigated in repair proficient and deficient human cells in situ . ^^^ Colocalization experiments of the NER factors showed that in normal human cells , XPC and TFIIH are rapidly and efficiently recruited to DNA damage within SNS . ^^^ By contrast , recruitment of both XPC and TFIIH to DNA damage in SNS occurred much less efficiently in p 53 Null or p 53 compromised cells . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| We propose a recognition mechanism by which the low specificity recognition factors , RPA , XPA , and XPC , act in a cooperative manner to locate the lesion and , aided by the kinetic proofreading provided by TFIIH , form a high specificity complex at the damage site that initiates removal of thymine dimers at a physiologically relevant rate and specificity . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Mutations in ERCC 1 , XPA , XPB , XPC , XPF , XPG and CSB dramatically reduced TER . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| In humans UV induced cyclobutane thymine dimers are excised by the joint action of six repair factors , RPA , XPA , XPC , TFIIH , XPG , and XPF . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Our recent repair studies in human cells reported that p 53 regulates the recruitment of XPC and TFIIH proteins to specific DNA damage sites . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| TFIIH is then recruited and , in the presence of ATP , extends the opening from position 6 to +6 ; it also displaces XPC downstream from the lesion , thereby providing the topological structure for recruiting XPA and RPA , which will enlarge the opening . ^^^ XPG and XPF / ERCC1 endonucleases then cut the damaged DNA at the limit of the opened structure that was previously `` labeled ' ' by the positioning of XPC / HR23B and TFIIH . . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| METHODS : Expression of CSA , CSB , XPC , hHR23B , XPA , XPB , ERCC 1 and p 53 genes was analyzed by quantitative RT PCR and immunoblotting in 26 HCC and 9 normal livers . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| The gene expression of the repair enzymes , XPA and XRCC 1 , was the same in all 3 cell lines but ERCC 1 , ERCC 3 and XPC were 2 6 times higher in AG 6000 compared to A 2780 cells . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| Evaluation of xeroderma pigmentosum XPA , XPC , XPD , XPF , XPB , XPG and DDB 2 genes in familial early onset lung cancer predisposition . ^^^ To formally investigate the role of XP related NER genes in lung cancer susceptibility , we screened germline DNA from 92 familial early onset lung cancer patients for mutations in all coding regions and intron exon boundaries of XPA , XPC , XPD , XPF , XPB , XPG and DDB 2 . ^^^ |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01831 and P19447 |
Pubmed |
SVM Score :0.0 |
| NA |
|