| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.70446242 |
| Here we show that the C terminal regulatory domain of Myt 1 specifically binds to p 90 ( rsk ) , a protein kinase that can be phosphorylated and activated by MAPK . p 90 ( rsk ) in turn phosphorylates the C terminus of Myt 1 and down regulates its inhibitory activity on p 34 ( cdc 2 ) / cyclin B in vitro . 0.70446242^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Myt 1 : a membrane associated inhibitory kinase that phosphorylates Cdc 2 on both threonine 14 and tyrosine 15 . ^^^ A complementary DNA was cloned from Xenopus that encodes Myt 1 , a member of the Wee 1 family that was discovered to phosphorylate Cdc 2 efficiently on both threonine 14 and tyrosine 15 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The results suggest that this thymus kinase is a novel Cdk inhibitory protein kinase , distinct from the recently cloned dual functional and membrane associated Cdc 2 inhibitory kinase , Myt 1 ( Mueller , P . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The human Myt 1 kinase preferentially phosphorylates Cdc 2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Human Myt 1 is a cell cycle regulated kinase that inhibits Cdc 2 but not Cdk 2 activity . ^^^ We find that human Myt 1 phosphorylates and inactivates Cdc 2 containing cyclin complexes but not complexes containing Cdk 2 or Cdk 4 . ^^^ Further , Cdc 2 . cyclin B 1 was capable of phosphorylating Myt 1 in vitro , but this phosphorylation did not affect Myt 1 kinase activity . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Myt 1 : a Wee 1 type kinase that phosphorylates Cdc 2 on residue Thr 14 . ^^^ The kinases responsible for these two phosphorylation events include the Myt 1 and Wee 1 kinases , which phosphorylate Cdc 2 on Thr 14 and Tyr 15 , respectively . ^^^ In this discussion , we summarise our current knowledge of the Myt 1 kinase and its regulation of Cdc 2 kinase activity during the G 2 to M phase transition . . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Overproduction of human Myt 1 kinase induces a G 2 cell cycle delay by interfering with the intracellular trafficking of Cdc 2 cyclin B 1 complexes . ^^^ The Myt 1 protein kinase functions to negatively regulate Cdc 2 cyclin B complexes by phosphorylating Cdc 2 on threonine 14 and tyrosine 15 . ^^^ Throughout interphase , human Myt 1 localizes to the endoplasmic reticulum and Golgi complex , whereas Cdc 2 cyclin B 1 complexes shuttle between the nucleus and the cytoplasm . ^^^ The COOH terminal 63 amino acids of Myt 1 were identified as a Cdc 2 cyclin B 1 interaction domain . ^^^ Myt 1 mutants lacking this domain no longer bound cyclin B 1 and did not efficiently phosphorylate Cdc 2 cyclin B 1 complexes in vitro . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Moreover , RINGO bound Cdc 2 and Cdk 2 are both less susceptible to inhibition by p 21 ( Cip 1 ) , whereas the Thr 14 / Tyr 15 kinase Myt 1 can negatively regulate the activity of Cdc 2 RINGO with reduced efficiency . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Here , we present the identification of Drosophila Myt 1 ( dMyt 1 ) kinase and examine the relationship of Myt 1 and Wee 1 activities in the context of cdc 2 phosphorylation . dMyt 1 kinase was found by BLAST searching the complete Drosophila genome using the amino acid sequence of human Myt 1 kinase . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Ectopic expression of the Drosophila Cdk 1 inhibitory kinases , Wee 1 and Myt 1 , interferes with the second mitotic wave and disrupts pattern formation during eye development . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Protein abundance for total Cdc 2 , Cdc 2 phosphorylated at the threonine 161 residue , Wee 1 , Myt 1 , Chk 1 , and 14 3 3sigma was not affected by treatment with ACNU in either cell line . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The C terminal domain of the Cdc 2 inhibitory kinase Myt 1 interacts with Cdc 2 complexes and is required for inhibition of G ( 2 ) / M progression . ^^^ In higher eukaryotes , Cdc 2 activity is in part regulated by inhibitory phosphorylation of Thr 14 and Tyr 15 , catalyzed by Wee 1 and Myt 1 , which prevents catastrophic premature entry into mitosis . ^^^ Truncation of the C terminal domain of Myt 1 prevented its ability to induce G ( 2 ) / M phase arrest in overexpression studies in human cells and dramatically reduced its ability to phosphorylate Cdc 2 in vitro . ^^^ We demonstrate that the C terminal domain of Myt 1 was required for recruitment of Cdc 2 , and we infer that this domain lies in the cytoplasm because it can interact with and is phosphorylated by Cdc 2 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| In uninfected cells the G ( 2 ) / M transition is regulated by cyclin kinase complex containing cdc 2 and , initially , cyclin A , followed by cyclin B . cdc 2 is downregulated through phosphorylation by wee 1 and myt 1 and upregulated by cdc 25C phosphatase . ^^^ In addition , a fraction of cdc 2 protein from infected cells exhibited altered electrophoretic mobility in denaturing gels . ( 3 ) The levels of cdk 7 or myt 1 proteins remained relatively constant throughout infection , whereas the level of wee 1 was significantly decreased . ( 4 ) cdc 25C formed novel bands characterized by slower electrophoretic mobility that disappeared after treatment with phosphatase . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| In Xenopus egg extracts , the inhibitory phosphorylations of Cdc 2 on Tyr 15 and Thr 14 are controlled by the phosphatase Cdc 25 and the kinases Myt 1 and Wee 1 . ^^^ At mitosis , Cdc 25 is activated and Myt 1 and Wee 1 are inactivated through phosphorylation by multiple kinases , including Cdc 2 itself . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The corresponding sites in the closely related Cdk 1 ( Thr 14 and Tyr 15 ) are of crucial importance for regulation of the G2 / M transition by myt 1 and wee 1 kinases and cdc 25 phosphatases . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The kinases , Chk 1 , Wee 1 , and Myt 1 are key regulators of this G ( 2 ) checkpoint , which act directly or indirectly to inhibit Cdc 2 activity . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Mos leads to activation of MAPK , Rsk , and the inhibition of the cdc 2 inhibitor Myt 1 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 regulation also involves positive feedback , with active Cdc 2 cyclin B stimulating its activator Cdc 25 ( refs 5 7 ) and inactivating its inhibitors Wee 1 and Myt 1 ( refs 8 11 ) . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of the G ( 2 ) / M regulatory proteins suggests that cdc 2 is involved in the cell cycle block by Myt 1 phosphorylation following 2 ME treatment . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| When Plk 1 was required for cyclin B Cdc 2 activation , the action of Plk 1 was mediated primarily through suppression of Myt 1 rather than through activation of Cdc 25 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| The decreased level of the Tyr 15 phosphorylated form of Cdk 1 in virus infected fibroblasts is due in part to the down regulation of the expression and activity of the Cdk 1 inhibitory kinases Myt 1 and Wee 1 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of WEE 1 is linked to ERK while phosphorylation of MYT 1 ( CDC 2 selective inhibitory kinase ) is tied to the ribosomal S 6 kinase ( RSK ) . ^^^ This results from the phosphorylation and subsequent inactivation of two inhibitory kinases ( WEE 1 and MYT 1 ) that tonically suppress CDK 2 and CDC 2 activity and activation of a phosphatase ( CDC25A ) that increases CDK 2 activity . . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| We report that when cyclin B is overexpressed in stage 4 oocytes , MPF autoamplification does not occur and the newly formed cyclin B Cdc 2 complexes are inactivated by Tyr 15 phosphorylation , indicating that Myt 1 kinase remains active and that Cdc 25 is prevented to be activated . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Keratinocyte G2 / M growth arrest by 1 , 25 dihydroxyvitamin D 3 is caused by Cdc 2 phosphorylation through Wee 1 and Myt 1 regulation . 1 , 25 dihydroxyvitamin D 3 ( 1 , 25 [ OH ] 2VD3 ) has an antiproliferative effect on keratinocyte growth , and its derivatives are used for the treatment of psoriasis . ^^^ These data indicated that the increase of Myt 1 and Wee 1 induced the phosphorylation of Cdc 2 leading to G2 / M arrest . ^^^ In conclusion , the induction of Cdc 2 phosphorylation due to the increase of Wee 1 and Myt 1 as well as the reduction of Cdc 2 and cyclin B 1 are involved in 1 , 25 [ OH ] 2VD3 induced G2 / M arrest of keratinocytes . . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| During the meiotic cell cycle in Xenopus oocytes , p 90 ( rsk ) , the downstream kinase of the Mos MAPK pathway , interacts with and inhibits the Cdc 2 inhibitory kinase Myt 1 . ^^^ At the M phase of the embryonic cell cycle , Cdc 2 phosphorylates Myt 1 on Thr 478 and thereby creates a docking site for Plx 1 . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| During resumption of meiosis in starfish oocytes , MYT 1 , a negative regulator of CDK 1 , is phosphorylated by PKB in an inhibitory manner . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Drosophila Myt 1 is a Cdk 1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis . ^^^ The metazoan Wee 1 like kinases Wee 1 and Myt 1 regulate the essential mitotic regulator Cdk 1 by inhibitory phosphorylation . ^^^ Despite the previously demonstrated role for Myt 1 regulation of Cdk 1 during meiosis , relatively little is known of how Myt 1 functions at other developmental stages . ^^^ Based on these observations , we propose that Myt 1 serves unique Cdk 1 regulatory functions required for efficient coupling of cell differentiation with cell cycle progression . . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| Identification of both Myt 1 and Wee 1 as necessary mediators of the p 21 independent inactivation of the cdc 2 / cyclin B 1 complex and growth inhibition of TRAMP cancer cells by genistein . ^^^ METHODS : Myt 1 and Wee 1 modulation by genistein was examined via Western blot analysis and the effect of their inhibition by siRNA on cyclin B 1 levels / localization , cdc 2 kinase activity , and cellular proliferation of genistein treated TRAMP C 2 cells was determined . ^^^ Downregulation of Myt 1 and Wee 1 by siRNA restored cdc 2 levels , its kinase activity , cyclinB 1 nuclear localization , and partially restored cell proliferation of genistein treated cells . ^^^ |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q01538 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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