| Interacting proteins: Q9UQ80 and Q01094 |
Pubmed |
SVM Score :0.0 |
| In an in vitro assay , these E2F1 Binding Proteins ( EBP 1 and EBP 2 ) associate with residues 1 121 of E2F1 or with the full length protein ; however , they do not associate with its carboxy terminus ( residues 88 437 ) . ^^^ When EBP 1 or EBP 2 were expressed in COS cells along with E2F1 and the target promoter DNA polymerase alpha , repression of transcription was observed . ^^^ However , no repression of DNA polymerase alpha was seen if the cells expressed a nonassociating mutant E2F1 ( residues 88 437 ) , along with EBP 1 or EBP 2 . ^^^ |
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| Interacting proteins: Q9UQ80 and Q01094 |
Pubmed |
SVM Score :0.0 |
| The overexpression of Ebp 1 in MCF 7 and AU 565 ( Rb ( + ) ) cells inhibited the activity of the E2F1 regulated cyclin E promoter . ^^^ Ebp 1 bound E2F1 indirectly via Rb in lysates of MCF 7 cells . ^^^ |
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| Interacting proteins: Q9UQ80 and Q01094 |
Pubmed |
SVM Score :0.0 |
| Repression of E2F1 mediated transcription by the ErbB 3 binding protein Ebp 1 involves histone deacetylases . ^^^ Ebp 1 binds the tumor suppressor retinoblastoma protein ( Rb ) both in vivo and in vitro , and Rb and Ebp 1 cooperate to inhibit the transcription of the E2F1 regulated cyclin E promoter . ^^^ The Ebp 1 mediated transcriptional repression depended on the presence of an E2F1 consensus element in the promoters . ^^^ |
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| Interacting proteins: Q9UQ80 and Q01094 |
Pubmed |
SVM Score :0.0 |
| Whereas GST Ebp 1 alone failed to bind E2F1 promoter elements , Ebp 1 contained in nuclear lysates associated with E2F1 consensus sequences in the E2F1 promoter . ^^^ Endogenous Ebp 1 was recruited to the E2F1 promoter in vivo as demonstrated by chromatin immunoprecipitation assays . ^^^ Ebp 1 bound E2F consensus oligonucleotides in association with E2F1 , retinoblastoma protein , and HDAC 2 . ^^^ |
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| Interacting proteins: Q9UQ80 and Q01094 |
Pubmed |
SVM Score :0.0 |
| The ErbB 3 binding protein Ebp 1 interacts with Sin3A to repress E2F1 and AR mediated transcription . ^^^ Ebp 1 inhibits transcription of E2F1 and androgen receptor regulated genes such as prostate specific antigen ( PSA ) through its interactions with histone deacetylases ( HDACs ) . ^^^ Chromatin immunoprecipitation ( ChIP ) and DNA affinity precipitation analysis demonstrated that Ebp 1 and Sin3A associate at the PSA and E2F1 promoters . ^^^ Functionally , Sin3A enhanced the ability of Ebp 1 to repress transcription of androgen receptor ( AR ) and E2F1 regulated genes . ^^^ |
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