Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.70494157
To study a possible interaction between E2F 1 and DP 1 , we have now isolated a cDNA for the human homolog of DP 1 . 0.70494157^^^ Finally , we demonstrate that E2F 1 and DP 1 association is required for stable interaction with pRB in vivo and that trans activation by E2F 1 / DP 1 heterodimers is inhibited by pRB . 0.5248002^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.57190038
In contrast , DP 1 was efficiently phosphorylated when weakly connected to cyclin A Cdk 2 via E2F1 or E2F4 with a fused cyclin A binding domain of E2F1 . 0.57190038^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Immunohistochemical expression of the transcription factor DP 1 and its heterodimeric partner E2F 1 in non Hodgkin lymphoma . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
A second E2F 1 binding protein , most likely DP 1 , was also apparently normal in both class 2 positive and negative B cell lines . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
DRTF1 / E2F is a heterodimeric DNA binding activity which arises when a member of two distinct families of proteins , DP and E2F , interact as DP / E2F heterodimers , for example , DP 1 and E2F 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
To test whether this site is functional , we cotransfected PCNA CAT genes with E2F 1 and DP 1 expression plasmids . ^^^ Expression of the E2F 1 / DP 1 heterodimer activated the CAT gene with the PCNA intron . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Here we show that MDM 2 makes a functional contact with two cooperating transcription factors , E2F1 and DP 1 ( refs 4 , 5 ) , which are involved in S phase progression . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In light of these findings , we have assayed the ability of the E2F1 and DP 1 genes , which encode heterodimeric partners that together create E2F activity , to act in an oncogenic fashion . ^^^ We find that E2F1 , particularly in combination with the DP 1 product , cooperates with an activated ras oncogene to induce the formation of morphologically transformed foci in primary rat embryo fibroblast cultures . ^^^ Cells transfected with E2F1 and DP 1 or the E2F1 VP 16 chimera form colonies in soft agar and induce tumor formation in nude mice . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Both DP 1 and DP 2 synergistically interact with members of the E2F family of proteins , E2F 1 , E2F 2 , and E2F 3 , to generate DNA binding complexes that specifically recognize the E2F site and functionally interact with E2F 1 in E2F site dependent transcriptional activation of cellular genes . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F 4 and 5 share common sequences with E2F 1 , E2F 2 , and E2F 3 and , like these other E2Fs , the ability to heterodimerize with DP 1 , thereby acquiring the ability to bind an E2F DNA recognition sequence with high affinity . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F 1 and DP 1 mRNAs were not detectable in granulocytes , monocytes and resting T lymphocytes but were induced after the mitogenic stimulation of T lymphocytes . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Mutually exclusive interaction of the adenovirus E 4 6 / 7 protein and the retinoblastoma gene product with internal domains of E2F 1 and DP 1 . ^^^ E2F DNA binding activity is composed of a heterodimer of related but distinct proteins of the E2F 1 and DP 1 families . ^^^ In this report , we have examined the regions of E2F 1 and DP 1 that are required for the induction of cooperative E2F binding to the E2a promoter by the E 4 6 / 7 protein . ^^^ DP 1 also is necessary for stable interaction with E 4 6 / 7 and an internal segment of DP 1 is required that is positioned in a location similar to that of the conserved E2F 1 domain . ^^^ Interestingly , the binding of E 4 6 / 7 and the binding of Rb to E2F are mutually exclusive , and our results show that the same internal segments of E2F 1 and DP 1 that are required for E 4 6 / 7 binding are also required for stable interaction with Rb . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Regulation of adenovirus 12 E1A transcription : E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1 , DP 1 , cyclin A and / or RB and mediate transcriptional ( auto ) activation . ^^^ For HeLa , 293 , U 937 , and A 549 cells , participation of E2F 1 , DP 1 , cyclin A , and RB was involved in formation of some complexes only , assuming participation of factors different from E2F 1 or DP 1 in others . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Cyclin A / CDK2 binds directly to E2F 1 and inhibits the DNA binding activity of E2F 1 / DP 1 by phosphorylation . ^^^ E2F 1 forms a heterodimer with DP 1 and binds to several cell cycle regulatory proteins , including the retinoblastoma family ( RB , p 107 , p 130 ) and cyclin A / CDK2 complexes . ^^^ Furthermore , an active DNA binding complex could be reconstituted from purified E2F 1 / DP 1 and cyclin A / CDK2 . ^^^ Because the stable association of E2F 1 with cyclin A / CDK2 in vitro and in vivo did not require a DP 1 or RB binding domain and because the interactions could be reconstituted from purified components in vitro , we conclude that the interactions between cyclin A / CDK2 and E2F 1 are direct . ^^^ Finally , we report that the DNA binding activity of the E2F 1 / DP 1 complex is inhibited following phosphorylation by cyclin A / CDK2 . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Heterodimerization of the transcription factors E2F 1 and DP 1 is required for binding to the adenovirus E 4 ( ORF6 / 7 ) protein . ^^^ Recent studies have shown that E2F is the combined activity of several proteins , and we demonstrate here that heterodimerization of two of these proteins , E2F 1 and DP 1 , is required for stable binding to E 4 . ^^^ This region of E2F 1 is conserved in E2F 2 and E2F 3 , and deletion of this region drastically reduces the transcriptional activity of the molecule without affecting DP 1 binding , suggesting that this region of the E2F transcription factors is involved in regulating their activity . ^^^ Our experiments also demonstrate that pRB binding to the E2F 1 / DP 1 heterodimer prevents the formation of an E2F 1 / DP 1 / E4 complex . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Two sequence specific DNA binding proteins , DP 1 and E2F 1 , are components of DRTF1 / E2F which synergistically interact in a DP 1 / E2F 1 heterodimer . ^^^ A C terminal region in DP 1 can interact with pRb which , in the context of the DP 1 / E2F 1 heterodimer , contributes to the efficiency of pRb binding . ^^^ The DP 1 / E2F 1 heterodimer specifically interacts with the adenovirus type 5 E 4 orf 6 / 7 protein , to produce a DNA binding activity which binds co operatively to , and transcriptionally activates through , two appropriately positioned E2F sites in a manner which resembles the regulation of DRTF1 / E2F by E 4 orf 6 / 7 during adenovirus infection . ^^^ We conclude that DP 1 is a frequent and cell cycle regulated component of DRTF1 / E2F , and that in the DP 1 / E2F 1 heterodimer it is functionally important for recognition by pRb and the E 4 orf 6 / 7 protein . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Although the recent isolation of molecular clones encoding proteins that are components of the E2F activity ( E2F1 and DP 1 ) provides an approach to defining the specific involvement of E2F in these events , definitive experiments remain difficult in the absence of appropriate genetic systems . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
However , these two proteins ( human E2F1 and mouse DP 1 ) are quite different in sequence . ^^^ To understand the role of the different E2F family members in the growth of mouse NIH 3T3 cells , we examined the levels of E2F1 and DP 1 mRNAs in different stages of the cell cycle . ^^^ Since the levels of E2F1 but not DP 1 mRNA correlated with changes in transcription from the dhfr promoter , we examined whether E2F1 could activate various growth regulated promoters . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Genes encoding two E2F proteins , E2F 1 and DP 1 , were regulated differently during the cell cycle and replicative senescence of normal human fibroblasts . ^^^ Senescent cells expressed DP 1 at the presenescent level , but did not express E2F 1 mRNA . ^^^ E2F 1 and DP 1 expression vectors only weakly induced DNA synthesis in quiescent or senescent human cells and immortal murine NIH3T3 cells , although the E2F 1 vector stimulated DNA synthesis in immortal murine A 31 cells , and transactivated E2F responsive promoters in NIH3T3 cells . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Interacting domains of E2F1 , DP 1 , and the adenovirus E 4 protein . ^^^ Recent experiments demonstrate that a family of related proteins constitute the E2F transcription factor activity and that the interaction of two of these gene products , E2F1 and DP 1 , generates a heterodimer with DNA binding and transcriptional activating capacity . ^^^ We now show that coexpression of the E2F1 and DP 1 products in transfected SAOS 2 cells , together with the E 4 product , generates a multicomponent complex with specificity to the adenovirus E 2 promoter . ^^^ Using a yeast two hybrid assay system , we find that the E2F1 hydrophobic heptad repeat ( E2F1 amino acid residues 206 to 283 ) allows interaction with a corresponding domain of the DP 1 protein ( amino acids 196 to 245 ) . ^^^ We also find that the adenovirus E 4 protein interacts with the DP 1 hydrophobic heptad repeat domain , but we could not detect a direct interaction between E2F1 and E 4 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Functional synergy between DP 1 and E2F 1 in the cell cycle regulating transcription factor DRTF1 / E2F . ^^^ Two sequence specific DNA binding proteins , E2F 1 and DP 1 , which bind to the E2F site , contain a small region of similarity . ^^^ We report here that DP 1 and E2F 1 exist in a DNA binding complex in vivo and that they bind efficiently and preferentially as a heterodimer to the E2F site . ^^^ Moreover , studies in yeast and Drosophila cells indicate that DP 1 and E2F 1 interact synergistically in E2F site dependent transcriptional activation . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
While at least two proteins ( E2F 1 and DP 1 ) with E2F like activity have been cloned , studies from several laboratories suggest that additional homologs may exist . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Here it is shown that two family members , E2F 1 and DP 1 , form specific heterodimers in vivo , a process that enhances DNA binding , transactivation , and the binding of the retinoblastoma gene product . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The recently cloned E2F related activities E2F 1 and DP 1 individually bind to an E2F binding site weakly , but when combined generate an activity that is indistinguishable from endogenous cellular E2F . ^^^ Recombinant E2F 1 , DP 1 , and E 4 6 / 7 are sufficient to form the induced E2F complex at the E2a promoter . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The DNA binding domain of DP 1 contains a region that resembles that of E2F 1 ( refs 16 , 17 ) , and recognizes the same sequence . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F 1 : DP 1 induces p 53 and overrides survival factors to trigger apoptosis . ^^^ However , overexpression of both E2F 1 and DP 1 led to the rapid death of cells even in the presence of survival factors . ^^^ In the presence of IL 3 , levels of endogenous wild type p 53 increased in response to E2F 1 , and coexpression of DP 1 further augmented p 53 levels . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Physical and functional interactions between p 53 and cell cycle co operating transcription factors , E2F1 and DP 1 . ^^^ We report here that there is a physical and functional interaction between p 53 and two of the components of the E2F transcription factors , E2F1 and DP 1 . ^^^ The expression of wild type p 53 can inhibit the transcriptional activity of E2F , and the expression of both E2F1 and DP 1 can also downregulate p 53 dependent transcription . ^^^ The transcriptional activity of p 53 is known to be inhibited by the direct binding of mdm 2 , but we demonstrate here that both E2F1 and DP 1 can inhibit p 53 transcriptional activity independently of mdm 2 . ^^^ Detailed studies of protein protein interactions have provided evidence that E2F1 and its co operating factor DP 1 can complex with p 53 both in vitro and in vivo . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F is a heterodimer composed of two partners , such as E2F1 and DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Combined microinjection of E2F 1 and DP 1 proteins or microinjected adenovirus E1A protein , however , could induce S phase in cells arrested in G 1 by PGA 2 , indicating that PGA 2 does not directly inhibit the process of DNA synthesis . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The E2F1 transcription factor , in co operation with DP 1 , controls the expression of several S phase specific genes . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Here , we show that the B myb repressor element is specifically recognised by heterodimers consisting of DP 1 and E2F 1 , E2F 3 or E2F 4 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The molecular basis of E2F 1 / DP 1 induced S phase entry and apoptosis . ^^^ Thus , the biological significance of the interaction of E2F 1 with its DP protein partner , DP 1 , was unclear . ^^^ Cells inducible for DP 1 , E2F 1 , or both were established and characterized . ^^^ In contrast , coexpression of DP 1 and E2F 1 results in greater loss of G 1 regulation and significantly more apoptosis than does E2F 1 alone . ^^^ Using clones co inducible for DP 1 and E2F 1 , expression of potential target genes of E2F activity that may account for its ability to induce S phase entry was also examined . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Expression of the E2F 1 / DP 1 transcription factor in murine development . ^^^ We demonstrate that the mRNA levels for E2F 1 and its heterodimeric partner DP 1 reach maximal levels in the late embryonic and early postnatal period but decline in the later postnatal and adult periods . ^^^ Furthermore , the unusual pattern of E2F 1 and DP 1 developmental expression may have an essential role in certain cells and tissues in the late embryonic and early postnatal period . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Generic DRTF1 / E2F DNA binding activity and transcriptional activation arise when members of two distinct families of proteins , such as DP 1 and E2F 1 , interact as DP / E2F heterodimers . ^^^ In vitro p 53 interacts with an immunochemically distinct form of DP 1 and in vivo can regulate transcription driven by the DP 1 / E2F 1 heterodimer . ^^^ At the biochemical level , p 53 competes with E2F 1 for DP 1 , with a consequent reduction in DNA binding activity . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Northern blot analysis of the expression of E2F and DP family members showed that the E2F 1 , E2F 4 , and E2F 5 mRNA was growth and senescence dependent , whereas E2F 3 , DP 1 , and DP 2 expression was constitutive and senescence independent . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In transfected cells , DP 1 did not accumulate in the nucleus unless it was coexpressed with the heterodimeric partners E2F 1 , E2F 2 , or E2F 3 . ^^^ Domain mapping experiments showed that regions of E2F 1 and DP 1 that are required for stable association of the two proteins were also required for nuclear localization of DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Cdk 3 bound specifically to E2F 1 / DP 1 complexes in vivo , most likely through DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F1 , the first cloned member of this family , is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product ( pRB ) , cyclin A and DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Down regulation of E2F 1 , a subunit of E2F , was observed after 8 h of culture with IFN alpha ; expression of E2F 4 , another subunit of E2F , and DP 1 , a heterodimeric partner of E2F , was unaffected . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In a first step toward elaborating the mechanism for cyclin D 1 mediated induction of p 21 gene expression we show that co expression of E2F 1 and DP 1 can specifically transactivate the p 21 promoter . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
We have shown that p 202 , an IFN inducible 52kDa primarily nuclear phosphoprotein whose expression in transfected cells inhibits cell proliferation , interacts with the retinoblastoma tumor suppressor protein ( pRb ) and the transcription factor E2F ( E2F 1 / DP 1 ) in vitro and in vivo . p 202 was shown to inhibit E2F 1 / DP 1 stimulated transcription of a reporter gene and of endogenous genes . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Examination of the expression levels for E2F genes and other genes associated with the cell cycle indicated that E2F1 was profoundly decreased in growth arrested keratinocytes ( 90 % ) , whereas E2F3 , E2F5 , and DP 1 were not . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
There is also recent evidence which suggests that mdm 2 may play roles in p 53 independent pathways regulating cellular proliferation . mdm 2 has recently been shown to interact with the retinoblastoma tumor suppressor protein p ( Rb ) , and the E2F 1 and DP 1 transcription factors . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Recombinant human E2F1 , E2F4 , and DP 1 fusion proteins were affinity purified from bacteria expressing these genes . ^^^ A combination of either E2F1 or E2F4 with their dimerization partner , DP 1 , gave preparations that exhibited binding to the E2F site containing DNA fragment . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Five E2F genes , E2F 1 through E2F 5 , and two DP genes , DP 1 and DP 2 , have been isolated from mammals , and heterodimeric complexes of these proteins are expressed in most , if not all , vertebrate cells . ^^^ Using recombinant E2F , DP , and Rb proteins prepared in baculovirus infected cells and a repetitive immunoprecipitation PCR procedure ( CASTing ) , we have identified consensus DNA binding sites for E2F 1 / DP 1 , E2F 1 / DP 2 , E2F 4 / DP 1 , and E2F 4 / DP 2 complexes as well as an Rb / E2F 1 / DP 1 trimeric complex . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
To investigate the role of E2F in murine development , we have examined the patterns of expression of E2F 1 through E2F 5 , and DP 1 in the developing nervous system by in situ hybridization . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In these cells , E2F 3 , E2F 4 , and DP 1 are regulated by both IL 3 and granulocyte colony stimulating factor ( G CSF ) , whereas E2F 1 was expressed at low levels and was not regulated by either cytokine . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
We found that anti IgM treatment induces the appearance of an inhibitory DNA binding complex containing the pRB related pocket protein p 130 together with E2F and a concomitant decrease in `` free ' ' E2F , consisting of E2F1 and its partner DP 1 ; these effects were reversed following stimulation via CD 40 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
This inhibitory activity has a relative specificity for the DP 1 promoter compared with the functionally related E2F1 promoter or unrelated promoters such as those of the transcription factor ATFa or the thymidine kinase gene . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
To test proliferative functions of E2F , we generated transgenic mice expressing human E2F 1 and / or human DP 1 . ^^^ Testicular atrophy as a result of overexpression of E2F 1 and DP 1 is independent of functional p 53 , since p 53 nullizygous transgenic mice overexpressing E2F 1 and DP 1 also suffered testicular atrophy . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
CAT reporter assays have shown that E2F 1 in association with its partner DP 1 transactivates its own promoter , whereas p105Rb inhibits the E2F 1 promoter . ^^^ Both E2F 1 / DP 1 and p105Rh require the presence of the E2F binding sites to mediate their effects . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In addition , overexpression of E2F 6 suppresses the transactivational effects of coexpression of E2F 1 and DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
This result , together with our previous work on the solution structure of a fragment of DP 1 , supports the proposal that E2F 1 and DP 1 may dimerize with a coiled coil type interaction . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The arrangement of amino acids in the helix 1 and helix 2 regions is quite similar to those of Mxi and Mad , but different from those of E2F 1 and DP 1 . ^^^ Western blot analysis of the immunoprecipitates prepared with anti E2FBP1 antibody showed that E2FBP1 associates with both E2F 1 and DP 1 in vivo . ^^^ E2FBP1 alone has no DNA binding activity , but bind to the E2F site through heterodimerization with E2F 1 but not with DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
This result supports the proposal that DP 1 and E2F 1 may dimerize with a coiled coil type interaction . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Association with E2F 1 governs intracellular trafficking and polyubiquitination of DP 1 . ^^^ In transient transfection experiments , DP 1 polypeptides that stably bound E2F 1 entered the nucleus . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Consistent with previous data from human and mouse fibroblasts and T lymphocytes , E2F4 and DP 1 form the predominant E2F heterodimers both in G 0 and G 1 phases of the human B lymphocyte cell cycle , whereas E2F1 and 3 are first detected in late G 1 , and their expression levels increase towards S phase . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Western blot analyses of whole cell extracts revealed that amounts of E2F4 , E2F1 , DP 1 , and p 107 remained unchanged after infection of C 33 cells . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Antibodies to DP 1 , E2F1 to 5 , p 107 , or pRb failed to either supershift or block E2Fmyb sp complex formation . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NeuT induction of the cyclin D 1 promoter required the E2F and Sp 1 DNA binding sites and was inhibited by dominant negative E2F 1 or DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Detailed analysis using a two hybrid approach in mammalian cells indicated lack of physical interaction between p 53 and E2F 5 , DP 1 , or E2F 1 . ^^^ Reciprocal analysis revealed that whereas E2F 1 dramatically inhibited p 53 activated transcription , E2F 5 or DP 1 did not . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Cotransfection of increasing concentrations of dominant negative E2F1 alone , or with dominant negative DP 1 into NIH3T3 cells suppressed induction of the p 21 promoter by activated Ras . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
E2F1 was the only family member more abundant in the melanoma cells compared with normal melanocytes , and the approximately fivefold increase in DNA binding activity could be accounted for mostly by a similar increase in the levels of the dimerization partner DP 1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Differential cytotoxic pathways of topoisomerase 1 and 2 anticancer agents after overexpression of the E2F 1 / DP 1 transcription factor complex . ^^^ The transcription factor complex E2F 1 / DP 1 regulates the G 1 to S phase transition and has been associated with sensitivity to the S phase specific anticancer agents camptothecin and etoposide , which poison DNA topoisomerase 1 and 2 , respectively . ^^^ To investigate the relationship between E2F 1 and drug sensitivity in detail , we established human osteosarcoma U 20S TA cells expressing full length E2F 1 / DP 1 under the control of a tetracycline responsive promoter , designated UE1DP 1 cells . ^^^ Topoisomerase 1 levels and activity as well as the number of camptothecin induced DNA single and double strand breaks were unchanged in UEIDP 1 / tc cells with > 10 fold E2F 1 / DP 1 overexpression . ^^^ This indicates that camptothecin induced toxicity in this model is due to the activation of an E2F 1 / DP 1 induced post DNA damage pathway rather than an increase in the number of replication forks caused by the S phase initiation . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Using tetracycline responsive promoters , here we compared the effects of ectopic expression of E2F 1 , DP 1 and E2F 4 on cell cycle progression and apoptosis in Chinese hamster cell lines . ^^^ We found that E2F 4 , as well as DP 1 and E2F 1 , induced growth arrest and caspase dependent apoptosis . ^^^ E2F 4 did not have a marked effect on cell cycle progression , while E2F 1 induced DNA synthesis of resting cells and DP 1 arrested cells in G 1 . ^^^ Our results suggest that expression of E2F 4 at elevated levels induces growth arrest and apoptosis of mammalian cells through a mechanism distinct from E2F 1 and DP 1 . . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Expression of pRB , cyclin / cyclin dependent kinases and E2F1 / DP 1 in human tumor lines in cell culture and in xenograft tissues and response to cell cycle agents . ^^^ Expression of pRb , cyclin D 1 , cdk 4 , cyclin E , cdk 2 , E2F1 and DP 1 was determined in MCF 7 and MDA MB 468 breast carcinoma cells , H 460 and Calu 6 non small cell lung carcinoma cells , H 82 and SW 2 small cell lung carcinoma cells , HCT 116 and HT 29 colon carcinoma cells and LNCaP and DU 145 prostate carcinoma cells . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
A functional E2F factor consists of a heterodimer containing an E2F polypeptide ( E2F1 E2F6 ) and a DRTF 1 polypeptide ( DRTF 1 polypeptide 1 ( DP 1 ) or DRTF 1 polypeptide 2 ) . ^^^ Coexpression of DP 1 with E2F1 or E2F4 in the epidermis of bigenic mice modestly enhanced proliferation and apoptosis over the levels induced by E2F1 or E2F4 expression alone . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
This function of E2F1 does not require its DP 1 binding , DNA binding , or transcriptional activity and is independent of mdm 2 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
No differences were observed between RA sensitive and RA resistant ovarian carcinoma cells in the levels of expression of many cell cycle genes including cyclin A , B and E , cdk 2 , 4 and 6 , E2F 1 , E2F 2 , E2F 3 , E2F 4 , E2F 5 , DP 1 and DP 2 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
In serum stimulated ( S phase ) cells , the composition of the complex switched to E2F1 / 4 , DP 1 and p 107 , together with cyclin A and cyclin dependent kinase 2 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Differential regulation of E2F1 , DP 1 , and the E2F1 / DP1 complex by ARF . ^^^ Coexpression of E2F1 and DP 1 blocks ARF induced relocalization of either subunit to the nucleolus . ^^^ Moreover , we show that E2F1 is more stable in the presence of ARF when coexpressed with DP 1 . ^^^ These results suggest that ARF differentially regulates the free and heterodimeric forms of E2F1 and DP 1 . ^^^ DP 1 is a constitutively expressed protein , whereas E2F1 is mainly expressed at the G ( 1 ) / S boundary of the cell cycle . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Expression of MDMX along with E2F 1 and Dp 1 in Saos 2 cells reduces the ability of E2F 1 to bind to its consensus DNA sequence , without altering E2F 1 protein levels . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Release of E2F1 / DP1 heterodimers from repression mediated by the retinoblastoma tumor suppressor ( pRB ) triggers cell cycle entry into S phase , suggesting that E2F1 and DP 1 proteins must act in unison , either to facilitate or to suppress cell cycle progression . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Thus , mRNA and protein levels of E2F 1 , 3 , and 4 and DP 1 and DP 2 were down regulated during development to undetectable levels in adult myocytes . ^^^ In contrast , an induction of E2F 1 , 3 , and 4 and the DP 1 protein was observed during the development of myocyte hypertrophy in neonatal myocytes treated with serum or phenylephrine , whereas the protein levels of E2F 5 were decreased with serum stimulation . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
We have isolated several peptides from random peptide phage display libraries that specifically recognize the cell cycle regulatory transcription factor E2F and inhibit DNA binding of E2F / DP heterodimers ( E2F 1 , E2F 2 , E2F 3 , E2F 4 or E2F 5 , and DP 1 ) . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Here we investigated the role of E2F1 and E2F4 expression , with or without co expression of DP 1 or DP 2 , on cell proliferation in transiently transfected primary rat MCs . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
The expressions of N myc , cyclin D 3 , and Wnt10B were downregulated , whereas those of retinoblastoma ( RB ) and related genes ( p 107 , RB2 / p130 , p300 / CBP , E2F 1 , DP 1 ) as well as others were upregulated . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
To assess transactivating activity of E2F1 / DP 1 , we also analyzed expression of ten putative transcriptional targets of this complex in HCCs . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Transcription activation of a complex consisting of E2F1 , DP 1 , and TRIP Br 1 was efficiently stimulated by both E 6 proteins . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
While an in vivo association of MdmX with either E2F1 or DP 1 was not observed , a slight reduction in DP 1 and an increased cytoplasmic localization of E2F1 were seen in cells overexpressing MdmX . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Identification and characterization of novel isoforms of human DP 1 : DP 1 { alpha } regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant negative manner . ^^^ Yeast two hybrid and immunoprecipitation assays demonstrated that DP 1alpha binding to E2F1 was significantly reduced as compared with that of wild type DP 1 or DP 1beta . ^^^ Immunofluorescence analysis revealed that the subcellular localization of both DP 1 isoforms changed from the cytoplasm to the nucleus in HEK 293 cells cotransfected with E2F1 and wild type DP 1 or DP 1beta . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Interestingly , binding of ARF to DP 1 results in an inhibition of the interaction between DP 1 and E2F1 . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
Structure of the Rb C terminal domain bound to E2F1 DP 1 : a mechanism for phosphorylation induced E2F release . ^^^ The crystal structure of an RbC E2F1 DP 1 complex reveals an intertwined heterodimer in which the marked box domains of both E2F1 and DP 1 contact RbC . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
We also found coinduction of several groups of related genes that might represent functional modules within the ischemic neuronal transcriptome , including VEGF and its receptor , NRP 1 ; the IGF 1 receptor and the IGF 1 binding protein IGFBP 2 ; Rb , the Rb binding protein E2F1 , and the E2F related transcription factor , TFDP 1 ; the CACNB 3 and CACNB 4 beta subunits of the voltage gated calcium channel ; and caspase 3 and its substrates , ACINUS , FEM 1 , and GSN . ^^^
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14186 and Q01094	Pubmed	SVM Score :0.0
NA