| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.75857406 |
| This event is linked to a specific interaction of E2F 1 with the F box containing protein p45SKP2 , which is the cell cycle regulated component of the ubiquitin protein ligase SCFSKP 2 that recognizes substrates for this ligase . 0.75857406^^^ Disruption of the interaction between E2F 1 and p45SKP2 results in a reduction in ubiquitination of E2F 1 and the stabilization and accumulation of transcriptionally active E2F 1 protein . 0.65896247^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| In contrast to substrates of the SKP 1 Cullin 1 F box ( SCF ) complexes , in vitro ubiquitination of E2F1 by CUL 1 ROC1 ligase does not require E2F1 phosphorylation , is not stimulated by overexpression of F box protein SKP 2 , and is not affected by immunodepletion of SKP 1 or mutations in CUL 1 disrupting SKPI binding . ^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| Of note , p 300 induced E2F 1 ubiquitination does not depend on the p45Skp2 E 3 ligase , since it does not extend to other p45Skp2 targets and also occurs with an E2F 1 mutant devoid of the p45Skp2 binding domain but still retaining the acetylated region . ^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| The enhanced E2F 1 stability is associated with the concomitant acetylation of E2F 1 , the disassociation of E2F 1 from the E2F 1 E 3 ligase p 45 ( SKP 2 ) , and decreased E2F 1 ubiquitination , suggesting CD 437 inhibition of E 3 E2F 1 ligase activity . ^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| We show that core protein suppresses the expression of tumor suppressor protein p 53 and cyclin dependent kinase ( CDK ) inhibitor p 21 and enhances the activation of cyclin dependent kinase 2 ( CDK 2 ) , the phosphorylation of retinoblastoma ( Rb ) , the activation of the transcription factor E2F 1 , and the expression of E2F 1 and S phase kinase interacting protein 2 ( SKP 2 ) genes . ^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| MDM 2 displaces SCF ( SKP 2 ) , the E2F1 E 3 ligase . ^^^ |
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| Interacting proteins: Q13309 and Q01094 |
Pubmed |
SVM Score :0.0 |
| Ectopic expression of E2F1 induces expression of the endogenous Skp 2 gene in human fibroblast cells , whereas antisense mediated knockdown of E2F1 in human tumor cell lines reduces expression of endogenous Skp 2 gene . ^^^ |
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