| Interacting proteins: Q00577 and Q01094 |
Pubmed |
SVM Score :0.94980361 |
| Results from GST pull down assays and the combined immunoprecipitation / Western blot analysis of nuclear extracts revealed a direct association of E2F 1 with Pur alpha in the absence of the DNA molecule containing the E2F 1 binding site . 0.94980361^^^ The association of Pur alpha with E2F 1 may increase the stability of E2F 1 , as a higher level of E2F 1 was detected in cells coexpressing Pur alpha and E2F 1 . 0.933148^^^ |
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| Interacting proteins: Q00577 and Q01094 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q00577 and Q01094 |
Pubmed |
SVM Score :0.0 |
| Interaction between the pura and E2F 1 transcription factors . ^^^ Previously , the multifunctional Pura protein was found to bind to E2F 1 and inhibit E2F 1 transcriptional activity . ^^^ MATERIALS AND METHODS : Pura deletion mutants were assayed for : in vitro binding to E2F 1 , inhibition of E2F 1 induced promoter activation and effects on cell proliferation . ^^^ Two RNA species with specific binding to E2F 1 and Pura were analyzed for their effects on E2F 1 / Pura binding and cell growth . ^^^ RESULTS : The N terminal 72 amino acids of Pura were involved in E2F 1 binding , inhibition of promoter activation by E2F 1 and reversal of E2F mediated growth inhibition . ^^^ |
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