Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.56487841
We have found that RBAP 1 copurifies with E2F , interacts specifically with the adenovirus E 4 ORF 6 / 7 protein , binds specifically and directly to a known E2F DNA recognition sequence , and contains a functional tranasactivation domain . 0.56487841^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.58120934
We now show that 14 3 3 tau , a phosphoserine binding protein , mediates E2F1 stabilization . 14 3 3 tau interacts with ATM phosphorylated E2F1 during DNA damage and inhibits E2F1 ubiquitination . 0.58120934^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A family with MEN 2A , consisting of 88 members and their spouses , was studied to test the reliability of the provocation of plasma calcitonin with pentagastrin and the possibility of DNA diagnosis of mutated gene carriers with DNA probes closely linked to the MEN2A gene including RBP 3 and FNRB genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In particular , two highly informative ( CA ) n dinucleotide repeat markers , sTCL 1 from proximal chromosome 10p ( 16 alleles , PIC = 0 . 68 ) and sJRH 1 from the RBP 3 locus ( 18 alleles , PIC = 0 . 88 ) , provide useful reagents for a polymerase chain reaction based predictive genetic test that can be performed rapidly from small amounts of DNA . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Nine chromosome 10 DNA markers ( FNRB , D10S34 , D10Z1 , MEN 203 , D10S94 , RBP 3 , D10S15 , MBP [ 48 . 11 ] , D10S22 ) were typed in two large Canadian pedigrees with multiple endocrine neoplasia type 2A ( MEN 2A ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The DNA markers , RBP 3 ( retinol binding protein 3 , interstitial ) and FNRB ( fibronectin receptor , beta polypeptide ) , are both tightly linked to the MEN2A locus , and are localized to opposite sides of the MEN2A locus . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We have also identified an activity , termed E2F 1 , that inhibits E2F binding to DNA , again apparently through the formation of a complex with E2F . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Strong positive lod scores and linkage distance relationships between D10Z1 and DNA markers from the chromosome 10 pericentromeric region , especially FNRB and RBP 3 , known to be on either side of the centromere , provide independent support for mapping of all these loci . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
By means of a set of ordered polymorphic DNA markers from the pericentromeric region , multipoint as well as pairwise linkage analyses place the MEN2A locus at the middle of a small region ( approximately 11 cM ) bracketing the centromere with FNRB ( at 10p11 . 2 ) and RBP 3 ( at 10q11 . 2 ) on either side , providing further support for the centromeric location of the MEN2A locus . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Combined somatic cell hybrid and linkage studies between D10S94 and five pericentromeric loci ( FNRB , D10Z1 , MEN2A , RBP 3 , and D10S15 ) have localized the new DNA sequence pcl1 / A1S 6 c 23 at D10S94 to 10q11 . 2 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The gene responsible for multiple endocrine neoplasia type 2A ( MEN2A ) has recently been assigned to the pericentromeric region of chromosome 10 in European Caucasian kindreds by linkage analysis using a DNA marker , interstitial retinol binding protein 3 ( RBP 3 ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Five chromosome 10 DNA markers ( D10S1 , D10S3 , D10S4 , D10S5 , and RBP 3 ) were typed in five large pedigrees with multiple endocrine neoplasia type 2A ( MEN 2A ) and in five non MEN 2A pedigrees . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A polymorphic DNA marker on chromosome 10 linked to RBP 3 on the MEN2A side . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We also demonstrate by in vitro pull down assays that the 72 kDa IE 1 protein can specifically interact with the DNA binding domain of E2F1 ( amino acids 88 to 191 ) in the presence of nuclear extract . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 and a cyclin like DNA repair enzyme , uracil DNA glycosylase , provide evidence for an autoregulatory mechanism for transcription . ^^^ The cell cycle dependent transcription factor , E2F 1 , regulates the cyclin like species of the DNA repair enzyme uracil DNA glycosylase ( UDG ) gene in human osteosarcoma ( Saos 2 ) cells . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
However , quantitation of DNA synthesis , during culture in serum deprived media , indicates that the E2F1d87 cell line synthesizes more DNA / cell as compared to the E2F1 cell line . ^^^ Consistent with this relative increase in DNA synthesis , the E2F1d87 cell line undergoes camptothecin induced apoptosis when cultured under conditions of serum starvation , while the control and E2F1 cell lines are unaffected by drug treatment under the same conditions . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Cellular targets for activation by the E2F1 transcription factor include DNA synthesis and G1 / S regulatory genes . ^^^ We find that many of the genes encoding S phase acting proteins previously suspected to be E2F targets , including DNA polymerase alpha , thymidylate synthase , proliferating cell nuclear antigen , and ribonucleotide reductase , are indeed induced by E2F1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DRTF1 / E2F is a heterodimeric DNA binding activity which arises when a member of two distinct families of proteins , DP and E2F , interact as DP / E2F heterodimers , for example , DP 1 and E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Previous studies using affinity chromatography techniques have shown that the activation domains of certain activators , including the acidic activation domain of E2F 1 , can interact with TBP in the absence of DNA . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Mutational analysis showed that the ability of E2F 1 to bind to DNA was necessary and sufficient to block the formation of large cells by RB , whereas the ability to induce S phase entry required a functional transactivation domain as well . ^^^ Furthermore , the ability of the E2F 1 DNA binding domain alone to block one manifestation of RB action is consistent with the notion that RB E2F complexes actively repress transcription upon binding to certain E2F responsive promoters . ^^^ In keeping with this view , we show here that coproduction of an E2F1 mutant capable of binding to DNA , yet unable to transactivate , is sufficient to block RB mediated transcriptional repression . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA synthesis is induced in adult neurons after expression of E2F1 and E1A . ^^^ Changing the tumor suppressor function of Rb by expressing transcription factor E2F1 and viral oncoprotein E1A in cerebellar granular neurons in vitro and in cerebral cortical neurons in vivo results in the induction of DNA synthesis in these neurons . ^^^ Immunoliposome mediated transfection of E1A and E2F1 cDNAs into the adult cortical neurons of rats in vivo results in initiation of DNA synthesis in 5 15 % of the transfected neurons . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We find that overexpression of E2F1 can overcome the TGF beta mediated effect as measured by the activation of cellular DNA synthesis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Both DP 1 and DP 2 synergistically interact with members of the E2F family of proteins , E2F 1 , E2F 2 , and E2F 3 , to generate DNA binding complexes that specifically recognize the E2F site and functionally interact with E2F 1 in E2F site dependent transcriptional activation of cellular genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F1 overexpression in quiescent fibroblasts leads to induction of cellular DNA synthesis and apoptosis . ^^^ Indeed , overexpression of the E2F1 product , a component of the cellular E2F activity , induces DNA synthesis in quiescent fibroblasts . ^^^ We demonstrate an induction of DNA synthesis when quiescent cells are infected with the E2F1 recombinant virus . ^^^ The incomplete nature of cellular DNA replication is due , at least in part , to the fact that E2F1 overexpression leads to massive cell death that is characteristic of apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 4 and 5 share common sequences with E2F 1 , E2F 2 , and E2F 3 and , like these other E2Fs , the ability to heterodimerize with DP 1 , thereby acquiring the ability to bind an E2F DNA recognition sequence with high affinity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F DNA binding activity is composed of a heterodimer of related but distinct proteins of the E2F 1 and DP 1 families . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
An E2F 1 fusion protein protects the sequence 5 ' GGATGGCGGGTAATA 3 ' from DNase 1 digestion , and a DNA probe containing this sequence binds an E2F specific protein complex from cell extracts , although this region is only loosely homologous with known consensus binding sites for E2F transcription factors . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Multiple DNA elements are required for the growth regulation of the mouse E2F1 promoter . ^^^ To elucidate the signal transduction pathway leading to the activation of genes required for DNA synthesis , we are investigating the mechanism by which expression of E2F1 is regulated . ^^^ However , the E2F sites are not sufficient to mediate growth regulated transcriptional activity ; our results indicate that multiple DNA elements are required for transcription regulation of the E2F1 promoter at the G1 / S phase boundary . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Overexpression of E2F 1 mutants that impair DNA binding or transactivation does not alter cell cycle progression or induce apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The growth inhibitory activity required the DNA binding function of E2F 1 but not its transactivation or pRB binding activities . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Complex formation results in a negative biochemical effect of cyclin A kinase : the shut off of E2F 1 dependent DNA binding function in S / G2 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Two sequence specific DNA binding proteins , DP 1 and E2F 1 , are components of DRTF1 / E2F which synergistically interact in a DP 1 / E2F 1 heterodimer . ^^^ The DP 1 / E2F 1 heterodimer specifically interacts with the adenovirus type 5 E 4 orf 6 / 7 protein , to produce a DNA binding activity which binds co operatively to , and transcriptionally activates through , two appropriately positioned E2F sites in a manner which resembles the regulation of DRTF1 / E2F by E 4 orf 6 / 7 during adenovirus infection . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The transformation caused by E2F 1 correlates with constitutive overexpression of the transgene , increased transcription of E2F dependent genes and the enhancement of two E2F DNA binding complexes containing the retinoblastoma susceptibility gene product ( Rb ) and E2F 1 . ^^^ The oncogenic potential of E2F 1 is dependent on functional DNA binding and transactivation domains but does not require the ability to interact directly with Rb . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Microinjection of a GST E2F 1 fusion protein into quiescent Balb / c 3T3 cells induced DNA synthesis whereas co injection of GST E2F 1 and GST E2F ( 95 191 ) protein , encoding only the DNA binding domain of E2F 1 , blocked the induction of S phase . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
By using this clone and a series of non pRB binding mutants , we have been able to show that the binding of pRB to E2F 1 causes inhibition of E2F mediated transactivation . pRB ' s inhibition of E2F mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours , by pRB ' s interaction with DNA tumour virus oncoproteins , or by phosphorylation during the cell cycle . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A cDNA clone was isolated from a Drosophila cDNA library by using a probe containing sequence from the E2F1 DNA binding domain . ^^^ The sequence of the clone , which we term drosE2F1 , demonstrates considerable homology to the human E2F1 sequence , with over 65 % identity in the DNA binding region and 50 % identity in the region of E2F1 known to interact with the retinoblastoma gene product . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We found that E2F1 could activate some ( dhfr , thymidine kinase , and DNA polymerase alpha ) but not all ( thymidylate synthase , cad , and c myc ) of these promoters . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Also in contrast to other bHLH proteins , mutations generated in conserved basic residues of E2F1 do not effect DNA binding . ^^^ Finally , E2F1 interacts with all of the G residues in the sequence GGCGGGAAA while the A residues are not required for DNA binding . ^^^ The uniqueness of the E2F1 DNA binding domain is likely to play a role in its binding a DNA site that is distinct from that of all other bHLH proteins ( CACGTG ) . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
To determine whether E2F 1 plays a role in the control of the cell proliferation , we introduced an inducible construct expressing an E2F 1 antisense RNA into the human glioblastoma T98G cell line and assessed DNA synthesis during the cell cycle . ^^^ Band shift analysis of bacterially produced E2F 1 showed that this protein bound to the promoters of human DNA polymerase alpha , cyclin D 1 , and c myb but not to the cdc 2 gene promoter . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 and DP 1 expression vectors only weakly induced DNA synthesis in quiescent or senescent human cells and immortal murine NIH3T3 cells , although the E2F 1 vector stimulated DNA synthesis in immortal murine A 31 cells , and transactivated E2F responsive promoters in NIH3T3 cells . ^^^ Furthermore , although E2F 1 stimulates DNA synthesis in some cells , other cells , including normal human fibroblasts , require additional factors . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Recent experiments demonstrate that a family of related proteins constitute the E2F transcription factor activity and that the interaction of two of these gene products , E2F1 and DP 1 , generates a heterodimer with DNA binding and transcriptional activating capacity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Two sequence specific DNA binding proteins , E2F 1 and DP 1 , which bind to the E2F site , contain a small region of similarity . ^^^ We report here that DP 1 and E2F 1 exist in a DNA binding complex in vivo and that they bind efficiently and preferentially as a heterodimer to the E2F site . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A novel protein with E2F like properties , designated E2F 2 , was cloned by screening a HeLa cDNA library with a DNA probe derived from the DNA binding domain of E2F 1 ( K . ^^^ Both the sequence and the function of the DNA and retinoblastoma gene product binding domains of E2F 1 are conserved in E2F 2 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Here it is shown that two family members , E2F 1 and DP 1 , form specific heterodimers in vivo , a process that enhances DNA binding , transactivation , and the binding of the retinoblastoma gene product . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Dual control of myc expression through a single DNA binding site targeted by ets family proteins and E2F 1 . ^^^ Analysis of E2F 1 sequences identified a minimal DNA binding domain that is related to those of ets proteins . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We found three genomic intervals that when deleted result in cessation of DNA replication in the embryo , 39D2 3 ; E 2 F1 , 51E and 75C5 7 ; F 1 . ^^^ Analysis of the effects of deletions in the 39D2 3 ; E 2 F1 region on DNA replication showed that the block to DNA replication correlates with deletion of the histone genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Specifically , we demonstrate that overexpression of the E2F1 complementary DNA can activate DNA synthesis in cells that would otherwise growth arrest , with an efficiency that is similar to that achieved by the expression of the adenovirus E1A gene . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The DNA binding domain of DP 1 contains a region that resembles that of E2F 1 ( refs 16 , 17 ) , and recognizes the same sequence . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Cyclin A kinase regulation of E2F 1 DNA binding function underlies suppression of an S phase checkpoint . ^^^ Here , we show that suppression of E2F 1 DNA binding activity by cyclin A kinase is linked to orderly S phase progression . ^^^ Disruption of this linkage resulted in S phase delay / arrest followed by regrowth or apoptosis , depending upon whether the DNA bound E2F 1 could transactivate . ^^^ Hence , the unscheduled presence of E2F 1 on specific DNA sequences during S phase can activate a specific S phase checkpoint , thereby linking transcription , DNA replication , and cell cycle control . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Although E2F1 can bind DNA as a homodimer and increase promoter activity , optimal DNA binding and transcriptional activity occurs in the heterodimeric form . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Combined microinjection of E2F 1 and DP 1 proteins or microinjected adenovirus E1A protein , however , could induce S phase in cells arrested in G 1 by PGA 2 , indicating that PGA 2 does not directly inhibit the process of DNA synthesis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Nuclear run on and RNase protection analyses revealed three classes of activation domains : Sp 1 and CTF stimulated initiation ( type 1 ) ; human immunodeficiency virus type 1 Tat fused to a DNA binding domain stimulated predominantly elongation ( type IIA ) ; and VP 16 , p 53 , and E2F1 stimulated both initiation and elongation ( type IIB ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA phasing analysis revealed that a consensus E2F site in the E2F1 promoter possesses an inherent bend with a net magnitude of 40 + / 2 degrees and with an orientation toward the major groove relative to the center of the E2F site . ^^^ Analysis of a series of E2F1 deletion mutants defined E2F1 sequences which are not required for DNA binding but are necessary for the DNA bending capacity of E2F . ^^^ An internal region of E2F1 , previously termed the marked box , which is highly homologous among E2F family members , was particularly important in DNA bending . ^^^ We also found that a bent DNA structure can be a contributory component in the activation of the E2F1 promoter but is not critical in the repression of that promoter in quiescent cells . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We demonstrate that E2F 1 strongly activated HSV TK , but in the absence of consensus E2F DNA elements . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Moreover , the simian virus 40 early promoter was rendered p 21 suppressible by introducing wild type , but not mutant , E2F binding sites ; p 21 deletion mutants showed good agreement in their abilities to inhibit E2F transactivation and DNA synthesis ; and E2F 1 ( which binds pRb ) , but not E2F 4 ( which does not ) , reversed both inhibitory effects of p 21 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In our analyses of the mechanisms involved in the control of glial cell proliferation in these tumor cells , we have focused our attention on E2F1 , a DNA binding transcription factor which modulates the activity of genes involved in the S phase of the cell cycle . ^^^ Of particular , unlike the classical E2F1 whose DNA binding activity is increased during S phase , the level GEAPs remained constant throughout the cell cycle . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Generic DRTF1 / E2F DNA binding activity and transcriptional activation arise when members of two distinct families of proteins , such as DP 1 and E2F 1 , interact as DP / E2F heterodimers . ^^^ At the biochemical level , p 53 competes with E2F 1 for DP 1 , with a consequent reduction in DNA binding activity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
When EBP 1 or EBP 2 were expressed in COS cells along with E2F1 and the target promoter DNA polymerase alpha , repression of transcription was observed . ^^^ However , no repression of DNA polymerase alpha was seen if the cells expressed a nonassociating mutant E2F1 ( residues 88 437 ) , along with EBP 1 or EBP 2 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We show that forced expression of a number of cell cycle regulatory genes , including erbB 2 , 5 ras , 5 myc , B myb , ld 1 , and E2F 1 , alone or in combinations , can not induce terminally differentiated skeletal muscle cells ( myotubes ) to synthesize DNA . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Transfection assay using different deletion constructs indicates that both the DNA binding and the transactivation domains of E2F1 are required for its inhibition of myoD transcription activation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In 32D abl cells , myc expression was found to be significantly higher than in the parental cells and was correlated with increased E2F 1 protein expression and DNA binding ability . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Used along with E1B to avert apoptosis , E2F 1 inhibited the cardiac and skeletal alpha actin promoters , serum response factor abundance , and sarcomeric actin biosynthesis , while inducing DNA synthesis and proliferating cell nuclear antigen . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We propose a dual mechanism of transcriptional repression by pRb which allows tight control of E2F1 responsive genes : a pRb E2F1 repressor unit is assembled off DNA to pre empt transcriptional activation by E2F1 ; recruitment of this repressor unit to cognate binding sites on promoters allows silencing of adjacent promoter elements . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We found no direct interaction between the E2F 1 protein and the RA response element in DNA or the RAR proteins . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 cooperates with topoisomerase 2 inhibition and DNA damage to selectively augment p 53 independent apoptosis . ^^^ Pretreating E2F 1 expressing cells with ICRF 193 , a second topoisomerase 2 inhibitor that does not damage DNA , protected the cells from etoposide induced apoptosis . ^^^ Therefore , topoisomerase 2 inhibition and DNA damage can cooperate to selectively induce p 53 independent apoptosis in cells that have unregulated E2F 1 activity resulting from mutations in the pRb pathway . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In order to determine whether E2F 1 that can not bind to RB might be associated with various human cancers , we searched for mutations in the RB binding domain of E2F 1 using samples of DNA from various clinical specimens obtained from 406 cancer patients ( with lung , pancreatic , stomach , colon , esophageal , and hepatic cancers ) by analysis of polymerase chain reaction mediated single strand conformational polymorphism . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Targeting E2F1 DNA complexes with microgonotropen DNA binding agents . ^^^ Microgonotropen ( MGT ) DNA binding drugs , which consist of an A+T selective DNA minor groove binding tripyrrole peptide and polyamine chains attached to a central pyrrole that extend drug contact into the DNA major groove , were found to be extraordinarily effective inhibitors of E 2 factor 1 ( E2F1 ) association with its DNA promoter element ( 5 ' TTTCGCGCCAAA ) . ^^^ A relationship was found between the measured equilibrium constants for binding of MGTs to the A+T region of d ( GGCGA3T3GGC ) / d ( CCGCT3A3CCG ) and their inhibition of complex formation between E2F1 and the DNA promoter element . ^^^ A representative of the potent MGT inhibitors was significantly more active on inhibition of E2F1 DNA complex formation compared with disruption of a preexisting complex . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We demonstrated that E2F mediated growth regulation of dhfr transcription requires activation of the dhfr promoter in S phase and that the C terminal activation domains of E2F1 , E2F4 , and E2F5 , when fused to the Gal 4 DNA binding domain , are sufficient to specify position dependent activation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A dominant negative mutant ( E2F97 ) of E2F1 containing the DNA binding domain of E2F1 under the control of a tetracycline responsive promoter was constructed . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Gel shift assays revealed that the DNA binding activity of free E2F , which is composed of E2F 1 and E2F 4 , was inhibited by IFN alpha . ^^^ In contrast , IFN alpha did not affect the DNA binding ability of E2F 1 and E2F 4 in a complex with retinoblastoma ( RB ) susceptibility gene family proteins including pRB , p 107 , and p 130 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Disruption of their binding to DNA with mutation in the E2F sites did not change the expression from promoters of E2F1 , B myb , or HsORC 1 genes in cycling HaCaT cells . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Binding of the E2F1 transcription factor to its DNA target is 50 % inhibited at approximately 2 nM concentration of 8 . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
However , we found that the requirement for E2F to cooperate with additional factors to achieve growth regulation could be relieved by bringing the E2F1 activation domain to the promoter via a Gal 4 DNA binding domain . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F1 induced apoptosis requires DNA binding but not transactivation and is inhibited by the retinoblastoma protein through direct interaction . ^^^ In contrast to E2F1 transactivation , the DNA binding activity of E2F1 was proven to be essential for its apoptotic function , as the DNA binding defective mutants E2F1 ( 132 ) and E2F1 ( 132 ) ( 1 374 ) failed to induce apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Induction of DNA synthesis and apoptosis are separable functions of E2F 1 . ^^^ Furthermore , this apoptotic function of E2F 1 is separable from the ability to accelerate entry into DNA synthesis . ^^^ Analysis of E2F 1 mutants indicates that although DNA binding is required , transcriptional transactivation is not necessary for the induction of apoptosis by E2F 1 , suggesting that it may be mediated through alleviation of E2F dependent transcriptional repression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Our results indicate that E2F DNA binding and in particular E2F1 mRNA expression are associated with keratinocyte proliferation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
These genes include : Acid phosphatase type 5 ( ACP 5 ) , Cholecystokinin Type B Receptor ( CCKBR ) , Antibiotic Peptide ( FALL 39 ) , Insulin like Growth Factor 1 Receptor ( IGF1R ) , Integrin Alpha M ( ITGAM ) , Integrin Beta 2 ( ITGbeta 2 ) , Opioid Receptor Mu 1 ( OPRM 1 ) , Pro hormone Converter ( PC1 / 3 ) , Retinol Binding Protein 3 ( RBP 3 ) , Ribosomal DNA ( RNR 1 ) , and Zona Pellucida Glycoprotein 1 ( ZP 1 ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
To stimulate DNA synthesis and reverse this growth arrest , we used an adenovirus vector to overexpress the transcription factor E2F1 in HSG cells . ^^^ These results suggest that E2F1 accumulation in growth arrested salivary gland cells can stimulate DNA synthesis and overcome a G0 / G1 block in the cell cycle . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Treatment of MM cells with MDM 2 antisense , but not sense , nonsense , or scrambled , oligodeoxyribonucleotides ( ODNs ) decreased DNA synthesis and cell viability ; it also induced G 1 growth arrest , as evidenced by propidium iodide ( PI ) staining and induction of retinoblastoma protein ( pRB ) to E2F 1 binding . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The results demonstrated that the growth inhibitory effects of 10 ( 8 ) M E 2 in ER stably transfected MDA MB 468 cells were associated with modulation of several factors required for cell cycle progression and DNA synthesis , including significant induction of the cyclin dependent kinase inhibitor p21cip 1 ( > 4 fold increase after 12 h ) and decreased E2F1 and PCNA protein levels . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
High affinity binding of the cell cycle regulated transcription factors E2F1 and E2F4 to benzo [ a ] pyrene diol epoxide DNA adducts . ^^^ A combination of either E2F1 or E2F4 with their dimerization partner , DP 1 , gave preparations that exhibited binding to the E2F site containing DNA fragment . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Affinity chromatography and co immunoprecipitation assays provide evidence for direct interaction of E2F 1 and p 50 in the absence of their DNA target sequences . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Using recombinant E2F , DP , and Rb proteins prepared in baculovirus infected cells and a repetitive immunoprecipitation PCR procedure ( CASTing ) , we have identified consensus DNA binding sites for E2F 1 / DP 1 , E2F 1 / DP 2 , E2F 4 / DP 1 , and E2F 4 / DP 2 complexes as well as an Rb / E2F 1 / DP 1 trimeric complex . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In cultured adult ventricular myocytes , adenoviral gene transfer of E2F 1 induced expression of proliferating cell nuclear antigen , cyclin dependent protein kinase 4 , cell division cycle 2 kinase , DNA synthesis , and apoptosis . ^^^ In vivo , adenoviral delivery of E2F 1 by direct injection into myocardium induced DNA synthesis , shown by 5 ' bromodeoxyuridine incorporation , and accumulation in G2 / M , by image analysis of Feulgen stained nuclei . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DDB , a putative DNA repair protein , can function as a transcriptional partner of E2F1 . ^^^ The transcription factor E2F1 is believed to be involved in the regulated expression of the DNA replication genes . ^^^ Here we show that DDB , a putative DNA repair protein , associates with the activation domain of E2F1 . ^^^ We show that the UV damaged DNA binding activity from HeLa nuclear extracts can associate with the activation domain of E2F1 . ^^^ For instance , DDB , which does not bind to the E2F sites , was enriched in the high salt fractions containing E2F1 during chromatography through an E2F specific DNA affinity column . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A comparison of DNA binding drugs as inhibitors of E2F1 and Sp 1 DNA complexes and associated gene expression . ^^^ In this study , we examined how DNA binding drugs prevented formation of transcription factor DNA complexes and influenced gene transcription from the hamster dihydrofolate reductase promoter , which is regulated by E2F1 and Sp 1 . ^^^ Gel mobility shift assay data showed that GC binding drugs ( e . g . , mitoxantrone ) inhibited the DNA binding of both E2F1 and Sp 1 . ^^^ In contrast , AT binding drugs ( e . g . , distamycin ) interfered only with E2F1 DNA complex formation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Indeed , overexpression of E2F proteins , including the E2F1 and E2F2 products , induces DNA synthesis in quiescent fibroblasts . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We found that anti IgM treatment induces the appearance of an inhibitory DNA binding complex containing the pRB related pocket protein p 130 together with E2F and a concomitant decrease in `` free ' ' E2F , consisting of E2F1 and its partner DP 1 ; these effects were reversed following stimulation via CD 40 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The DNA binding domain , the pRB pocket binding region , and the amino terminal Sp 1 binding domain of E2F 1 were required for full repression of cyclin D 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A vector expressing E2F1 was capable of increasing DNA synthesis in rat salivary glands , though complete mitosis was not observed . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Microinjection of recombinant proteins such as dominant negative E2F or universal Cdk inhibitors , p 21 and p 27 , but not wild type human E2F 1 or Cdk 4 specific inhibitor , p 19 , into maturing oocytes during MI abolished induction of the DNA replication ability . ^^^ Co injection of human E2F 1 and cyclin E proteins into immature oocytes allowed them to initiate DNA replication even in the absence of progesterone treatment . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Depletion of PARP also prevented induction of the expression of the transcription factor E2F 1 , which positively regulates transcription of the DNA pol alpha and PCNA genes ; thus , PARP may be necessary for expression of these genes when quiescent cells are stimulated to proliferate . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The transcription of a number of these genes , including E2F1 and B myb , is repressed in G0 / early G 1 at E2F DNA binding sites mediated by interaction of E2F with the Rb family member proteins . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
This coordinated sequence of signaling events is focused on E2F regulation so that , downstream of the pocket proteins , WC 1 stimulation results in a diminished DNA binding activity for free E2F as a consequence of reduced E2F1 expression , whereas E2F4 expression is unaffected . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In contrast , p 107 is dephosphorylated and is then depleted from cells as they exit the cell cycle . p 130 , predominantly in Form 1 , and hypophosphorylated pRb bind an E2F DNA binding site ; p 130 complexes E2F 4 , whereas pRb binds both E2F 4 and E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We found that a novel yeast protein , Xtc1p , could be covalently crosslinked to the activation domain of LexA E2F 1 when this derivatized activator was bound to DNA upstream of an activator responsive RNA polymerase 2 promoter . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Infection of HSG cells with an adenoviral vector encoding E2F1 , either 12 h before or immediately following irradiation , but not post irradiation , induced maintenance of cells in the S phase of the cell cycle , reduced the number of cells arrested at G2 / M , and decreased the rate of appearance of cells with < 2n DNA . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Initiation of proliferation but not of differentiation was followed by a 20 to 25 fold increase in the nuclear level of the DNA polymerase associated processivity factor PCNA and of the proliferation specific transcription factor E2F1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Moreover , we demonstrated that exogenous overexpression of either HsCdc 6 or HsOrc 1 failed to induce DNA synthesis unlike overexpression of E2F1 , even though E2F1 induced both Cdc 6 and Orc 1 , suggesting that E2F may regulate the expression of another gene ( s ) , besides Cdc 6 and Orc 1 , required for induction of cellular DNA synthesis in mammalian cells . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2FBP1 alone has no DNA binding activity , but bind to the E2F site through heterodimerization with E2F 1 but not with DP 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Increased levels of E2F 1 dependent DNA binding activity after UV or gamma irradiation . ^^^ These activities depend on the ability of E2F 1 to form functionally active DNA binding complexes . ^^^ Here we describe an assay that allows one to measure E2F 1 DNA binding activity in naive cells . ^^^ We find that DNA damage , generated by UV or gamma irradiation , prompts increased production of E2F 1 DNA binding activity , which , at least in part , originates from alterations in E2F 1 protein levels . ^^^ These findings represent an indication for a role of the transcription factor E2F 1 in the DNA damage response pathway . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We show here that E2F 1 protein levels increase in human medulloblastoma , glioma , lung , colon , and bladder cancer cell lines ( n=7 ) following treatment with the DNA damaging agents adriamycin or etoposide . ^^^ Although E2F 1 protein levels increase following DNA damage , several genes transcriptionally targeted by E2F 1 are not similarly induced . ^^^ Correspondingly , fibroblasts from E2F 1 knockout mice are more resistant to DNA damage than cells from normal mice . ^^^ Overexpression of E2F 1 protein in tumor cell lines infected with an adenovirus encoding wild type E2F 1 leads to enhanced cytotoxicity following exposure to DNA damaging agents , which results from enhanced apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Furthermore , gel shift assays showed that E2F 1 DNA binding was constitutive in AT cells , whereas it was inhibited in control cells following exposure to ionizing radiation . ^^^ The data suggests that abnormalities in the function of Rb and E2F 1 proteins may also be responsible for the failure of AT cells to arrest in the G1 / S checkpoint in response to DNA damage . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Increased cyclin E cdk 2 protein expression was accompanied by the inhibition of DNA synthesis , with a decrease in E2F 1 expression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
JNK 1 phosphorylates E2F1 in vitro , and co transfection of JNK 1 reduces the DNA binding activity of E2F1 ; treatment of cells with TNFalpha had a similar effect . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Negative regulation of E2F 1 DNA binding function by cyclin A kinase represents part of an S phase checkpoint control system that , when activated , leads to apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Results from DNA fragmentation and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling analyses indicated apoptosis induction in cells infected with AdCMV E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Transcription factor E2F 1 is upregulated in response to DNA damage in a manner analogous to that of p 53 . ^^^ We show here that E2F 1 protein is upregulated in response to DNA damage . ^^^ Upregulation of E2F 1 in response to DNA damage seems to require the presence of wild type p 53 , since we did not observe an increase in the level of E2F 1 protein in several cell lines which possess mutated p 53 . ^^^ These data suggest that E2F 1 is upregulated in a similar way to p 53 in response to DNA damage and that Mdm 2 appears to play a major role in this pathway . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
When subconfluent growing cells expressing this mutant E2F1 are analyzed in detail , it is shown here that they display a significantly reduced incorporation of 3H thymidine into the DNA of each S phase cell , compared to control cells or to cells overexpressing full length E2F1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Surprisingly , there was no new expression of PCNA and DNA pol alpha , as well as the transcription factor E2F 1 in PARP antisense cells during entry into S phase , suggesting that PARP may play a role in the expression of these proteins , perhaps by interacting with a site in the promoters for these genes . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
METHODS : Tumor samples from 133 evaluable patients with bladder cancer were analyzed for E2F 1 gene mutations by use of polymerase chain reaction single strand conformational polymorphism ( PCR SSCP ) analysis and DNA sequencing . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
To detect E2F binding in ExoIII or BssHII protection PCR assays , the use of 3 . 13 fmol ( 5 . 00 ng ) or 2 . 33 fmol ( 4 . 62 ng ) of DNA ( containing E 2 promoters ) and 0 . 325 microg ( 3 . 70 pmol ) or 0 . 175 microg ( 2 . 00 pmol ) of GST E2F 1 protein , respectively , were found to be sufficient . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
BRLF 1 induced activation of E2F1 may be required for efficient EBV lytic replication , since at least one critical viral replication gene ( the viral DNA polymerase ) is activated by E2F ( C . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Transcriptional activation of E2F1 gene expression by 17beta estradiol in MCF 7 cells is regulated by NF Y Sp1 / estrogen receptor interactions . 17beta Estradiol ( E 2 ) stimulated proliferation and DNA synthesis in MCF 7 human breast cancer cells , and this was accompanied by induction of E2F1 mRNA and protein levels . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Rb binding by E2F 1 was neither necessary nor sufficient for G 1 exit , whereas DNA binding was required ; thus , exogenous E2F 1 did not merely function by competing for the Rb `` pocket . ' ' E2F 1 induced G 1 exit was blocked by the `` universal ' ' Cdk inhibitor p 21 but not by p 16 , a specific inhibitor of Cdk4 / 6 ; p 21 was permissive for E2F 1 induction of cyclins E and A , but prevented their stimulation of Cdk 2 kinase activity . ^^^ Thus , E2F 1 induced Cdk 2 function was necessary , although not sufficient , to trigger DNA synthesis in cardiac muscle cells . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Poly ( ADP ribose ) polymerase upregulates E2F 1 promoter activity and DNA pol alpha expression during early S phase . ^^^ E2F 1 , a transcription factor implicated in the activation of genes required for S phase such as DNA pol alpha , is regulated by interactions with Rb and by cell cycle dependent alterations in E2F 1 abundance . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
First , E2F1 directly transcribes genes that are necessary for DNA synthesis , and second , it promotes cell cycle progression via the induction of G 1 cyclins . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 is a DNA binding transcription factor which , upon interaction with its target DNA sequence , induces expression of several S phase specific genes allowing progression of the cell cycle . ^^^ In this study , we have demonstrated that expression of a sequence specific single stranded DNA binding protein , Pur alpha , in cells decreases the ability of E2F 1 to exert its transcriptional activity upon the responsive promoter derived from DHFR . ^^^ Results from band shift experiments revealed that while Pur alpha does not recognize the double stranded DNA fragment containing the E2F 1 binding site , it has the ability to inhibit E2F 1 interaction with its target DNA sequence . ^^^ Results from GST pull down assays and the combined immunoprecipitation / Western blot analysis of nuclear extracts revealed a direct association of E2F 1 with Pur alpha in the absence of the DNA molecule containing the E2F 1 binding site . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NeuT induction of the cyclin D 1 promoter required the E2F and Sp 1 DNA binding sites and was inhibited by dominant negative E2F 1 or DP 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In contrast , E2F1 is not required for the induction of apoptosis by glucocorticoids or DNA damage . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA content analysis and bromodeoxyuridine pulse chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML 1B expressing 32D . 3 myeloid progenitor cells as compared with control cells or cells expressing E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In this study , we generated stably transfected mouse NIH3T3 cells that express exogenous human E2F 1 under the control of a heavy metal inducible metallothionein promoter and analyzed the molecular mechanism of the E2F 1 mediated initiation of chromosomal DNA replication . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Because cell cycle progression is dependent on the transcriptional activity of E2F family members ( E2F1 E2F6 ) , most of them regulated by suppressive association with pocket proteins , we characterized E2F pocket protein DNA binding activity in normal versus malignant human melanocytes . ^^^ In contrast , in melanoma cells , free E2F DNA binding activity ( E2F2 and E2F4 , to a lesser extent E2F1 , E2F3 , and occasionally E2F5 ) , was constitutively maintained at high levels independently of external melanocyte mitogens . ^^^ E2F1 was the only family member more abundant in the melanoma cells compared with normal melanocytes , and the approximately fivefold increase in DNA binding activity could be accounted for mostly by a similar increase in the levels of the dimerization partner DP 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Acetylation of E2F 1 in vitro and in vivo markedly increases its binding affinity for a consensus E2F DNA binding site , which is paralleled by enhanced transactivation of an E2F responsive promoter . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The suppression of E2F 1 was associated with ( 1 ) dephosphorylation of retinoblastoma susceptibility gene proteins , pRB and p 130 , and ( 2 ) accumulation of E2F pRB and E2F p 130 DNA binding complexes that bind to the E2F consensus sequence located in the E2F 1 promoter . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Exposure to CD 437 results in enhanced E2F 1 binding to its DNA consensus sequences and transcriptional activity during S phase . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The transcription factor complex E2F 1 / DP 1 regulates the G 1 to S phase transition and has been associated with sensitivity to the S phase specific anticancer agents camptothecin and etoposide , which poison DNA topoisomerase 1 and 2 , respectively . ^^^ Topoisomerase 1 levels and activity as well as the number of camptothecin induced DNA single and double strand breaks were unchanged in UEIDP 1 / tc cells with > 10 fold E2F 1 / DP 1 overexpression . ^^^ This indicates that camptothecin induced toxicity in this model is due to the activation of an E2F 1 / DP 1 induced post DNA damage pathway rather than an increase in the number of replication forks caused by the S phase initiation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Whereas ectopic Myc and E2F1 rescue the G ( 1 ) / S delay caused by pRbDeltacdk ( or dnDP 1 ) and MadMyc , respectively , cyclin E or Cdc25A can restore DNA replication even in cells concomitantly exposed to pRbDeltacdk and MadMyc . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Inhibition of Ras mediated transformation was dependent upon E2F DNA binding activity but did not require amino or carboxy terminal E2F1 protein interaction domains . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Roles of poly ( ADP ribosyl ) ation and PARP in apoptosis , DNA repair , genomic stability and functions of p 53 and E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We showed here that the expression level of E2F 1 can be up regulated by the treatment of DNA damage agents as well as hypoxia . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Furthermore , overexpressing E2F1 alone using replication deficient recombinant adenovirus was sufficient to cause neuronal cell death by apoptosis , as evidenced by the appearance of hallmarks of apoptosis , such as the threefold increase in caspase 3 like activity and increased laddered DNA fragmentation , in situ endlabeled DNA fragmentation , and numbers of neuronal cells with punctate nuclei . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Here we show that the predominant mammalian DNA methyltransferase , DNMT 1 , co purifies with the retinoblastoma ( Rb ) tumour suppressor gene product , E2F1 , and HDAC 1 and that DNMT 1 cooperates with Rb to repress transcription from promoters containing E2F binding sites . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The results show that ( 1 ) at 8 h postinfection or thereafter , E2F 1 and E2F 5 were posttranslationally modified and / or translocated from nucleus to the cytoplasm , ( 2 ) E2F 4 was hyperphophorylated , and ( 3 ) overall , E2F binding to cognate DNA sites was decreased at late times after infection . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 4 did not have a marked effect on cell cycle progression , while E2F 1 induced DNA synthesis of resting cells and DP 1 arrested cells in G 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NPDC 1 , a regulator of neural cell proliferation and differentiation , interacts with E2F 1 , reduces its binding to DNA and modulates its transcriptional activity . ^^^ This interaction reduces the binding of E2F 1 to DNA and its transcriptional activity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Although E2F1 and p 53 mediate ectopic DNA synthesis and cell death in several tissues in Rb mutant embryos , both endoreduplication and apoptosis persisted in mgRb : Rb / : E2F1 / and mgRb : Rb / : p 53 / compound mutant muscles . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Cytokine receptors activate signals that regulate the transcription factor E2F 1 , which then coordinates the expression of genes essential for DNA synthesis and cell cycle progression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We now show that cultured adult rat sensory neurons can replicate DNA in response to ectopic expression of E2F1 or E2F2 and that this is augmented by expression of cyclin dependent kinase activities . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
STUDY SELECTION : One manuscript reviewed the development of pharmacogenetics , 3 described analytic approaches to detect aneuploidy or cancer , 1 described transcription factor E2F 1 increase during apoptosis , 2 reported on genetic and pharmacologic factors that influence platelet aggregation , 2 described molecular methods for detecting long QT syndrome or mycobacteria , and 1 reported a modification in collection of buccal DNA . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Concomitantly , E2F1 activity and cellular DNA synthesis capacity were significantly reduced . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Pretreatment of the E2F 1 expressing cells with ICRF 193 , a second topoisomerase 2 inhibitor that does not cause DNA damage , protected these cells against etoposide induced apoptosis . ^^^ An E2F 1 mutant that is defective in inducing cell cycle progression also induced p 53 , suggesting that p 53 was responding directly to E2F , and not to secondary events caused by inappropriate cell cycle progression ( i . e . , DNA damage ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Selective induction of E2F1 in response to DNA damage , mediated by ATM dependent phosphorylation . ^^^ We now show that DNA damage leads to a specific induction of E2F1 accumulation , dependent on ATM kinase activity and that the specificity of E2F1 induction reflects a specificity in the phosphorylation of E2F1 by ATM as well as the related kinase ATR . ^^^ Finally , we also show that E2F1 is required for DNA damaged induced apoptosis in mouse thymocytes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The E2F1 transcription factor modulates neuronal apoptosis induced by staurosporine , DNA damage and beta amyloid . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Then using Western blot analysis to find the presence HPVl6E7 protein and pRb E2F 1 complex in HPV 16 DNA positive cervical carcinoma tissues . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Using an E2F 1 deletion mutant ( E2F 1 ( 180 437 ) ) we show that E2F 1 targeted degradation of MDMX does not require the E2F 1 DNA binding domain and therefore is independent of E2F 1 driven transcription . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Expression by recombinant adenovirus of E2F1 , E2F2 , E2F3 , cyclin E / cdk2 , and Mdm 2 individually resulted in DNA synthesis in 10 30 % of cells . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Analysis of immortal myeloid cells engineered to overexpress c Myc and E2F 1 DNA binding mutants revealed that DNA binding activity of these oncoproteins is required to suppress Bcl 2 expression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We demonstrate here that the E2F1 induced by DNA damage can bind to and promote the apoptotic function of p 53 via the cyclin A binding site of E2F1 . ^^^ This function of E2F1 does not require its DP 1 binding , DNA binding , or transcriptional activity and is independent of mdm 2 . ^^^ However , in response to DNA damage , cyclin A levels decrease , with a concomitant increase in E2F1 p 53 complex formation . ^^^ These results suggest that the binding of E2F1 to p 53 can specifically stimulate the apoptotic function of p 53 in response to DNA damage . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
More E2F 1 expression was induced at the hepatic G1 / S boundary just prior to each peak of DNA synthesis in regenerating livers of CR mice ( P < 0 . 01 ) , and 8 hr earlier among CR mice . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We used DNA microarrays and cell lines containing either inducible E2F 1 or inducible E2F 3 to identify novel E2F target genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We also show that this transcriptional repression is direct and dependent upon E2F1 ' s DNA binding domain , but does not require the transactivation domain of E2F1 . ^^^ Consistent with this DNA binding requirement of E2F1 to repress Mcl 1 , we show that E2F1 binds to the Mcl 1 promoter both in vitro and in vivo , and have identified the DNA element ( 143 / 117 ) within this promoter that is required for E2F1 binding and repression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Greater cell cycle and DNA synthesis activity of BM CD34+ than PB CD34+ cells were reflected by the 2 to 5 fold higher expression of 9 genes involved in cell cycle progression , 11 genes regulating DNA synthesis , and cell cycle initiating transcription factor E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Differences in DNA binding properties between E2F1 and E2F4 specify repression of the Mcl 1 promoter . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Expression analysis by cDNA microarray and RT PCR revealed novel E2F1 target genes involved in E2F1 regulated cellular functions such as cell cycle control , DNA replication and apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
This observation is further supported with assays showing that E2F 1 binding to specific DNA sequences is enhanced in MTT mdm 2 cells . ^^^ This negative regulation correlates with an increase in the percentage of the cell population with DNA content below 2N , suggesting that E2F 1 promotes apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Gel shift assays in the presence of an antibody demonstrate the presence of E2F1 in the protein DNA complexes generated on the MP segment . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The amino terminus of the E2F 1 transcription factor inhibits DNA replication of autonomously replicating plasmids in mammalian cells . ^^^ The E2F1 transcription factor plays a pivotal role in driving cells out of a quiescent state and into the S phase of the cell cycle , in part by transactivating genes needed for DNA replication including DHFR , thymidine kinase , and DNA Polymerase alpha . ^^^ Further , inhibition of replication is dependent on both the amino terminus of the E2F1 protein and on a DNA sequence that is contained within the 3 ' end of the E2F1 cDNA . ^^^ These data provide a clue to the mechanism by which E2F1 regulates transit through the S phase checkpoint , by acting on a specific DNA sequence via its amino terminal region , to inhibit elongation of DNA replication . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In the central nervous system , loss of E2F1 or E2F3 can almost completely eliminate the ectopic DNA replication and apoptosis observed in Rb ( / ) embryos , and loss of E2F2 partially reduces the unscheduled DNA replication and has no effect on apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
While Mdm 2 association with p 53 was required to increase E2F1 transactivation , Mdm 2 mediated degradation of p 53 was not . p 53 repression of E2F1 transactivation required a functional DNA binding and transactivation domain . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F1 can signal p 53 phosphorylation in the absence of p19ARF , similar to the observed modifications to p 53 in response to DNA damage . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In view of this dichotomy in its functions and its critical role in cell cycle control , this study examined the following four aspects of E2F 1 in a panel of 87 non small cell lung carcinomas ( NSCLCs ) , previously analysed for defects in the pRb p 53 MDM2 network : firstly , the status of E2F 1 at the protein , mRNA and DNA levels ; secondly , its relationship with the kinetic parameters and genomic instability of the tumours ; thirdly , its association with the status of its transcriptional co activator CBP , downstream target PCNA and main cell cycle regulatory and E2F 1 interacting molecules pRb , p 53 and MDM 2 ; and fourthly , its impact on clinical outcome . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Cyclin D 1 , a product of the bcl 1 gene , phosphorylates the retinoblastoma protein , releasing E2F 1 , which in turn activates genes involved in DNA synthesis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Ectopically expressed E2F 1 activated the hTERT promoter through a noncanonical DNA binding site . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Hoechst 33342 induced apoptosis is associated with disruption of TATA box binding protein / TATA box complexes , replication protein A / single stranded DNA complexes , topoisomerase I / DNA cleavable complexes and with an increased intracellular concentration of E2F 1 transcription factor and nitric oxide concentration . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The region within E2F 1 needed for this association is adjacent to the DNA binding domain . ^^^ It appears that this interaction reduces the ability of E2F 1 to bind DNA . ^^^ Expression of MDMX along with E2F 1 and Dp 1 in Saos 2 cells reduces the ability of E2F 1 to bind to its consensus DNA sequence , without altering E2F 1 protein levels . ^^^ These data indicate that the MDMX protein is capable of associating with E2F 1 and negatively regulating its DNA binding ability . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 playing a key role in G 1 to S phase progression was phosphorylated in vitro by DNA PK . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells . ^^^ We conclude that RBF 1 forms complexes with both E2F1 / DP and E2F2 / DP that cooperate to repress the expression of pre RC genes , which helps confine DNA synthesis to sites of gene amplification . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The transcription factor E2F1 that is expressed in granule neurons was found in DNA binding assays to bind to the EBE of the cdc 2 gene . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The level of topoisomerase 1 expression was not affected by combination treatment as well , suggesting that DNA replication and topoisomerase 1 activity may not account for the molecular mechanism of cell killing in response to Ad E2F 1 / camptothecin treatment . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The latter was observed by the reduction in the phosphorylated forms of pRb , p 107 and p 130 , and the formation of growth suppressive DNA binding complexes consisting of pRb and E2F1 or E2F3 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Thus , the binding of p202a results in the inhibition of the sequence specific binding to DNA of the c Fos , c Jun , E2F1 , E2F4 , MyoD , myogenin , and c Myc transcription factors . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The apoptotic function of E2F 1 is dependent on its ability to bind DNA ; cyclin A kinase activity has been shown to negatively regulate the DNA binding capacity of E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Our prior studies demonstrated selective induction and stabilization of E2F1 through ATM dependent phosphorylation in response to DNA damage . ^^^ Here we report that DNA topoisomerase IIbeta binding protein 1 ( TopBP 1 ) regulates E2F1 during DNA damage . ^^^ This interaction was induced by DNA damage and phosphorylation of E2F1 by ATM . ^^^ Thus , the specific interaction between TopBP 1 and E2F1 during DNA damage inhibits the known E2F1 activities but recruits E2F1 to a BRCA 1 containing repair complex , suggesting a direct role of E2F1 in DNA damage checkpoint / repair at stalled replication forks . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Chk 2 activates E2F 1 in response to DNA damage . ^^^ The E2F 1 transcription factor is regulated during cell cycle progression and induced by cellular stress , such as DNA damage . ^^^ We report that checkpoint kinase 2 ( Chk 2 ) regulates E2F 1 activity in response to the DNA damaging agent etoposide . ^^^ A Chk 2 consensus phosphorylation site in E2F 1 is phosphorylated in response to DNA damage , resulting in protein stabilization , increased half life , transcriptional activation and localization of phosphorylated E2F 1 to discrete nuclear structures . ^^^ Therefore , Chk 2 phosphorylates and activates E2F 1 in response to DNA damage , resulting in apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Moreover , we found that the DNA binding domain and the trans activation domain of E2F1 are important in mediating suppression of differentiation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
These changes were accompanied by increased expression of S and G ( 2 ) phase cyclins and cyclin dependent kinases ( cdk ) , increased Rb phosphorylation , and increased E2F 1 DNA binding activity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Differential regulation of E2F1 apoptotic target genes in response to DNA damage . ^^^ E2F1 is stabilized in response to DNA damage but it has not been established how this translates into the activation of specific subsets of E2F target genes . ^^^ Here , we applied a chromatin immunoprecipitation approach to show that , in response to DNA damage , E2F1 is directed from cell cycle progression to apoptotic E2F target genes . ^^^ We identify p 73 as an important E2F1 apoptotic target gene in DNA damage response and we show that acetylation is required for E2F1 recruitment on the P1p73 promoter and for its transcriptional activation . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The greater cell cycle and DNA synthesis activity of BM CD 34 ( + ) compared to PB CD 34 ( + ) cells was reflected by the 2 to 5 fold higher expression of 9 genes involved in cell cycle , 11 genes regulating DNA synthesis , and the cell cycle initiating transcription factor E2F 1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Like the expression of the known E2F activators , E2F1 , E2F2 , and E2F3 , the expression of E2F7 is growth regulated , at least in part , through E2F binding elements on its promoter , and its protein product is localized to the nucleus and associates with DNA E2F recognition sites with high affinity . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We have isolated several peptides from random peptide phage display libraries that specifically recognize the cell cycle regulatory transcription factor E2F and inhibit DNA binding of E2F / DP heterodimers ( E2F 1 , E2F 2 , E2F 3 , E2F 4 or E2F 5 , and DP 1 ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Previous work has demonstrated a role for the E2F1 gene product in signaling apoptosis , both as a result of the deregulation of the Rb / E2F pathway as well as in response to DNA damage . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
In response to DNA damage , E2F 1 is induced and phosphorylated . ^^^ Phosphorylated E2F 1 can reside in discrete nuclear structures and induce apoptosis , suggesting a unique role for E2F 1 in DNA repair and checkpoint functions . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
A decrease in neuronal damage after mild ischemia in E2F1 null mice was observed by immunohistochemical monitoring of the loss in neuronal specific microtubule associated protein 2 cytoskeletal protein and the appearance of nuclear DNA fragmentation by terminal deoxynucleotidyl transferase mediated 2 ' deoxyuridine 5 ' triphosphate biotin nick end labeling . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
This induction was abolished when a mutated form of E2F1 , not able to bind DNA , was used . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F1 / pRB complexes formed through this site have low affinity for DNA , but the interaction is sufficient for pRB to regulate E2F1 induced apoptosis , and E2F1 loses the ability to interact with this site following DNA damage . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Similarly to what observed for Raf / MEK / ERK activation and E2F 1 gene expression , the inhibition of PKC as well as its down regulation , significantly lowered the DNA synthesis induced by N LDL in serum free HECs . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA damage induces transcriptional activation of p 73 by removing C EBPalpha repression on E2F1 . p 73 is a member of the p 53 family often overexpressed in human cancer . ^^^ We also show that C EBPalpha has a direct repressive activity on transfactor E2F1 , and for this repression the binding of C EBPalpha to its consensus sequence in the DNA is required . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The phosphorylation of E2F 1 is markedly diminished , whereas that of the retinoblastoma protein is minimally affected , so that E2F 1 / DP 1 heterodimers remain bound to DNA . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
PARP 1 binds E2F 1 independently of its DNA binding and catalytic domains , and acts as a novel coactivator of E2F 1 mediated transcription during re entry of quiescent cells into S phase . ^^^ Mapping of the interaction domains revealed that full length PARP 1 as well as PARP 1 mutants lacking either the catalytic active site or the DNA binding domain equally bind E2F 1 , whereas a PARP 1 mutant lacking the automodification domain does not , suggesting that the protein interaction site is located in this central domain . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E1a expression led to marked increases in the transcription factor E2F , and overexpression of E2F 1 allowed proliferation in hypoxic cells , although it had minimal effect on the anoxic suppression of DNA initiation . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
When CDK 2 activity was bypassed by activation of the ER E2F1 fusion protein , cAMP no longer inhibited expression of Cdc25A or cyclin A but still inhibited DNA synthesis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
E2F 1 is a transcription factor regulating the expression of TS along with other crucial DNA synthesis related enzymes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The hallmark molecular profile of MM cells before their commitment to SAHA induced cell death is a constellation of antiproliferative and / or proapoptotic molecular events , including down regulation of transcripts for members of the insulin like growth factor ( IGF ) / IGF 1 receptor ( IGF 1R ) and IL 6 receptor ( IL 6R ) signaling cascades , antiapoptotic molecules ( e . g . , caspase inhibitors ) , oncogenic kinases , DNA synthesis / repair enzymes , and transcription factors ( e . g . , XBP 1 , E2F 1 ) implicated in MM pathophysiology . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Leukocyte DNA was analyzed for mutations in the exons of the genes encoding cellular retinaldehyde binding protein 1 ( RLBP 1 ) , 11 cis retinol dehydrogenase ( RDH 5 ) , interphotoreceptor retinoid binding protein ( RBP 3 ) , and photoreceptor all trans retinol dehydrogenase ( RDH 8 ) . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Here we define a transcription mediated pathway in which deregulation of E2F1 by ectopic E2F expression or Rb inactivation by E 7 of human papillomavirus type 16 signals apoptosis by inducing the expression of Chk 2 , a component of the DNA damage response . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
This regulation is crucial in the control of E2F1 dependent apoptosis during normal cell growth and DNA damage . ^^^ Thus , TopBP 1 functions as a critical modulator and serves as a negative feedback regulator of E2F1 by inhibiting E2F1 dependent apoptosis during G1 / S transition as well as DNA damage to promote cell survival . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
To investigate genomic mechanisms involved in estrogenic effects on the primate brain in vivo , we compared transcription factor mRNA and protein expression in the DLPFC of ovariectomized rhesus monkeys treated with either vehicle or estradiol ( E 2 ) . c FOS , E2F1 , and general transcription factor IIB ( TFIIB ) mRNA and protein expression were altered significantly by short term E 2 treatment , as shown by DNA array , in situ hybridization , and immunohistochemical and immunoblot evaluations . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Here , we describe a novel connection between E2F1 and the ATM DNA damage response pathway . ^^^ In contrast , ATM status has no effect on transcriptional activation of target genes or the stimulation of DNA synthesis by E2F1 . ^^^ Cells containing mutant Nijmegen breakage syndrome protein ( NBS 1 ) , a component of the Mre 11 Rad50 DNA repair complex , also have attenuated p 53 phosphorylation and apoptosis in response to E2F1 expression . ^^^ Delayed gammaH2AX phosphorylation and absence of ATM autophosphorylation at Ser 1981 suggest that E2F1 stimulates ATM through a unique mechanism that is distinct from agents that cause DNA double strand breaks . ^^^ These findings identify new roles for several DNA damage response factors by demonstrating that they also participate in the oncogenic stress signaling pathway between E2F1 and p53 . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The expression of genes involved in DNA replication and cell cycle control was upregulated in the E2F1 / E2F2 compound mutant pancreas , suggesting that their expression is repressed by E2F1 / E2F2 activities and that the inappropriate cell cycle found in the mutant pancreas is likely the result of the deregulated expression of these genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Life , death and E2F : linking proliferation control and DNA damage signaling via E2F1 . ^^^ Recently , we described the requirement for the DNA damage response proteins Atm , Nbs 1 , and Chk 2 in the E2F1 apoptosis pathway . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
As E2f1 is inducible by DNA damage we investigated its importance in vivo in ultraviolet ( UV ) induced apoptosis , a protective mechanism that prevents the epidermis from accumulating UV induced mutations . ^^^ These results imply that E2f1 functions as a suppressor of an apoptosis pathway that is initiated by DNA photoproducts and perhaps genetic abnormalities ; p 53 relieves this suppression . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The emerging role of E2F 1 in the DNA damage response and checkpoint control . ^^^ A central role for the E2F 1 transcription factor in the DNA damage response pathway is gaining support . ^^^ E2F 1 is phosphorylated by DNA damage responsive protein kinases , which leads to E2F 1 accumulation and the induction of apoptosis . ^^^ In addition , emerging information suggests that E2F 1 may play a role in the detection and subsequent repair of damaged DNA . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We showed that the expression of E2F 1 targets involved in G1 / S transit , DNA replication , and mitosis is not altered during the hypertrophic response , while the expression of E2F 1 regulated genes controlling early G 1 progression , cytoskeletal organization , protein synthesis , mitochondrial function , and programmed cell death is up regulated . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
This promoter region lacks a consensus E2F binding site , suggesting that E2F1 may be recruited to the DNA in a unique fashion . ^^^ To address whether E2F1 directly bound to this non consensus site , we demonstrated that the DNA binding domain of E2F1 is necessary for E2F1 mediated activation of the carboxylesterase promoter . ^^^ We propose a model in which E2F1 specific regulation of the carboxylesterase promoter requires both E2F1 / DNA interactions and protein protein interaction between E2F1 and a factor that binds adjacent to the non consensus site . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The other NSC compounds caused DNA damage and apoptosis and reduced E2F1 levels . ^^^ We conclude DNA damage and reductions in E2F1 protein are mechanistically important to the differentiation and antiproliferative activities of these quinoline drug candidates . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The hypothesis is proposed that deoxythymidine triphosphate ( dTTP ) allosterically feedback controls E2F 1 to regulate both DNA synthesis and apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Amplification of genomic DNA by the polymerase chain reaction ( PCR ) showed no E2F1 gene amplification or mutation in the Rb binding region of E2F1 in MDS patients , nor was transcriptional up regulation noted when E2F1 messenger RNA ( mRNA ) levels were estimated with real time reverse transcriptase PCR . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The subunit p 62 is a structural component of the TFIIH core that is known to interact with VP 16 , p 53 , Eralpha , and E2F1 in the context of activated transcription , as well as with the endonuclease XPG in DNA repair . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Unexpectedly , although the alkylating agent cisplatin also induced degradation of Cdc25A ( albeit delayed , after 8 12 h ) , cyclin E / CDK2 activity was elevated and DNA synthesis continued , a phenomena that correlated with increased E2F1 protein levels and consequently enhanced expression of cyclin E . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We demonstrate as well that E2F1 directs p 73 expression in the presence and absence of DNA damage . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Divergent siblings : E2F2 and E2F4 but not E2F1 and E2F3 induce DNA synthesis in cardiomyocytes without activation of apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Regulation of epidermal apoptosis and DNA repair by E2F1 in response to ultraviolet B radiation . ^^^ The E2F1 transcription factor regulates the expression of genes involved in cell proliferation , apoptosis and DNA repair . ^^^ Following DNA damage , E2F1 is phosphorylated and stabilized , but the physiological role of E2F1 in the response to DNA damage is unclear . ^^^ Instead , inhibition of UVB induced apoptosis by E2F1 correlates with a stimulation of DNA repair . ^^^ Mice lacking E2F1 are impaired for the removal of DNA photoproducts , while E2F1 transgenic mice repair UVB induced DNA damage at an accelerated rate compared to wild type mice . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Opioid agonist induced regulation of E2F1 DNA binding activity in NG 108 15 cells . ^^^ Gel shift assays and Western blotting of nuclear extracts from NG 108 15 cells revealed increased E2F1 DNA binding activity and higher levels of E2F1 following activation of delta opioid receptors . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Regulation of E2F 1 after DNA damage by p 300 mediated acetylation and ubiquitination . ^^^ By using an antibody specifically recognizing the acetylated form of E2F 1 ( AcE2F 1 ) , we found that , after DNA damage , AcE2F 1 accumulates in the cells in a time dependent manner , and that acetylation is increased by the expression of p 300 . ^^^ Remarkably , the same DNA damaging conditions also induce the accumulation of ubiquitinated E2F 1 , an event that is again markedly stimulated by p 300 overexpression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Recently , an E2F1 mutant lacking the DNA binding domain , E2F1 ( 180 437 ) , has been implicated in degradation of MDMX and MDM 2 proteins via lysosomal proteases . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
To improve the sensitivity of this system , we evaluated many two hybrid format synthetic gene constructs in which the GAL 4 DNA binding domain was fused to the ligand binding domain of the Choristoneura fumiferana EcR mutant V390I / Y410E ( GEvy ) , and various activation domains VP 16 , p 53 , p 65 , or E2F 1 were fused to the EF domains of chimeric human RXR . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
BACKGROUND : The retinoblastoma ( Rb ) pathway , which governs cell cycle progression , is frequently genetically altered in cancer , causing deregulated expression of the E2F 1 transcription factor , which promotes DNA synthesis and cell cycle progression . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The aCGH results were compared to gene expression data obtained using DNA microarrays and suggested the involvement of caspases and ICEBERG on 11q and E2F1 on chromosome 20q . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Rb inactivation leads to E2F1 mediated DNA double strand break accumulation . ^^^ We show here that pRb inactivation and the resultant deregulation of one E2F family member , E2F1 , leads to DNA double strand break ( DSB ) accumulation in normal diploid human cells . ^^^ Thus , Rb status is key to regulating both the proliferation promoting functions associated with E2F and for preventing DNA damage accumulation if E2F1 becomes deregulated . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
MDM 2 inhibits tumor suppressor property of pRb , by releasing E2F1 , which stimulates DNA synthesis in S phase . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Gene expression profiles that distinguish either E2F1 or E2F3 expressing cells from quiescent cells are enriched in genes encoding cell cycle and DNA replication activities , consistent with many past studies . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation ( ChIP ) and DNA affinity precipitation analysis demonstrated that Ebp 1 and Sin3A associate at the PSA and E2F1 promoters . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA damage responsive acetylation of pRb regulates binding to E2F 1 . ^^^ Here , we report that the acetylation of pRb K873 / 874 occurs in response to DNA damage and that acetylation regulates the interaction between the C terminal E2F 1 specific domain of pRb and E2F 1 . ^^^ These results define a new role for pRb acetylation in the DNA damage signalling pathway , and suggest that the interaction between pRb and E2F 1 is controlled by DNA damage dependent acetylation of pRb . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Here , we show that E2F1 expression results in MRN foci formation , which is independent of the Nbs 1 interacting region and the DNA binding domain of E2F1 . ^^^ E2F1 induced MRN foci are similar to irradiation induced foci ( IRIF ) that result from double strand DNA breaks because they correlate with 53BP1 and gammaH2AX foci , do not form in NBS cells , do form in AT cells and do not correlate with cell cycle entry . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Yet , it is unclear whether early programs of apoptotic injury that involve membrane phosphatidylserine ( PS ) exposure and calreticulin expression as well as later phases of apoptotic injury with nuclear DNA injury require the critical modulation of Rb and E2F1 . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Oncogenes and the DNA damage response : Myc and E2F1 engage the ATM signaling pathway to activate p 53 and induce apoptosis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
DNA binding independent cell death from a minimal proapoptotic region of E2F 1 . ^^^ This revealed that only 75 amino acids from within the DNA binding domain of E2F 1 is sufficient for cell death and that this activity is also present in the DNA binding domains of E2F 2 and E2F 3 . ^^^ However , analysis of this domain from E2F 1 revealed it does not bind DNA and is consequently unable to transactivate , repress or de repress E2F target genes . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The main targets of p 21 ( Waf 1 ) are Cyc 1E Cdk 2 and Cyc 1A Cdk 2 complexes , PCNA ( proliferating cell nuclear antigen ) , a subunit of DNA polymerase delta , and E2F 1 transcription factor . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
The transcription factor E2F1 coordinates cell cycle progression and induces apoptosis in response to DNA damage stress . ^^^ Aside from DNA damage , the role of E2F1 in the endoplasmic reticulum ( ER ) stress signaling pathways is unclear . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
We recently reported that the expression of dbpA ( DNA binding protein A ) is associated with advanced stages of human hepatocellular carcinoma ( HCC ) and that its transcription is positively regulated by E2F1 , which is also implicated in hepatocarcinogenesis . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
Interactions between E2F1 and SirT 1 regulate apoptotic response to DNA damage . ^^^ DNA damage by etoposide causes E2F1 dependent induction of SirT 1 expression and knockdown of SirT 1 increases sensitivity to etoposide . ^^^ These results reveal a mutual regulation between E2F1 and SirT 1 that affects cellular sensitivity to DNA damage . . ^^^
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and Q01094	Pubmed	SVM Score :0.0
NA