| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.71219448 |
| Here we show that Sp 1 and Sp 3 cooperate with E2F 1 to activate the MYCN promoter . 0.71219448^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.61096796 |
| Because of the large number of growth regulated genes containing binding sites for the transcription factors Sp 1 and E2F and the reported ability of E2F to mediate cell cycle ( growth ) regulation , we studied interactions between E2F1 and Sp 1 . 0.61096796^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Nonetheless , E2F 1 could bind to GC rich elements , which were conclusively identified in classic studies of HSV TK as SP 1 sites . ^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Ectopic expression of E2F 1 repressed hTERT promoter activity by inhibiting Sp 1 activation of the hTERT promoter . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Nuclear run on and RNase protection analyses revealed three classes of activation domains : Sp 1 and CTF stimulated initiation ( type 1 ) ; human immunodeficiency virus type 1 Tat fused to a DNA binding domain stimulated predominantly elongation ( type IIA ) ; and VP 16 , p 53 , and E2F1 stimulated both initiation and elongation ( type IIB ) . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| For this interaction , the C terminal part of Sp 1 and the N terminus of E2F1 , a domain also present in E2F2 and E2F3 but absent in E2F4 and E2F5 , were essential . ^^^ Accordingly , E2F1 to E2F3 but not E2F4 and E2F5 were found to bind sp 1 in vitro . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| In human C33A cells , expression of a transfected reporter gene driven by a GC box containing fragment of the human E2F1 promoter was enhanced by co transfection of an Sp 1 expression plasmid . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| A comparison of DNA binding drugs as inhibitors of E2F1 and Sp 1 DNA complexes and associated gene expression . ^^^ In this study , we examined how DNA binding drugs prevented formation of transcription factor DNA complexes and influenced gene transcription from the hamster dihydrofolate reductase promoter , which is regulated by E2F1 and Sp 1 . ^^^ Gel mobility shift assay data showed that GC binding drugs ( e . g . , mitoxantrone ) inhibited the DNA binding of both E2F1 and Sp 1 . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| The DNA binding domain , the pRB pocket binding region , and the amino terminal Sp 1 binding domain of E2F 1 were required for full repression of cyclin D 1 . ^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Sequences between 119 and 60 basepairs containing four Sp 1 consensus elements and two noncanonical E2F binding sites are of major importance for E2F activation , although E2F1 and E2F3 differ in the extent of their ability to activate expression when this segment is deleted . ^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Repression assays revealed that WT 1 represses all three classes of activation domains : Sp 1 and CTF , which stimulate initiation ( type 1 ) , human immunodeficiency virus type 1 Tat fused to a DNA binding domain , which stimulates predominantly elongation ( type IIA ) , and VP 16 , p 53 and E2F1 , which stimulate both initiation and elongation ( type IIB ) . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Previously we have shown that the C terminus of Sp 1 is necessary for the interaction with the transcription factor E2F1 ( J . ^^^ Coexpression of E2F1 interferes with HDAC 1 binding to Sp 1 and abolishes Sp 1 mediated transcriptional repression . ^^^ Our results indicate that one component of Sp 1 dependent gene regulation involves competition between the transcriptional repressor HDAC 1 and the transactivating factor E2F1 . . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Gel mobility shift assays with multiple oligonucleotides and protein antibodies ( for supershifts ) showed that the 146 to 54 region of the E2F1 gene promoter bound Sp 1 and NF Y proteins in MCF 7 cells . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Cyclin A , which binds to E2F 1 in close vicinity to Sp 1 does not interfere with this interaction . ^^^ Moreover we have investigated the ability of other members of the Sp 1 family to interact with E2F 1 and to regulate the activity of the E2F and Sp 1 dependent murine thymidine kinase promoter . ^^^ All four factors of the Sp 1 family are able to bind E2F 1 in co immunoprecipitation and GST pull down experiments . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| NeuT induction of the cyclin D 1 promoter required the E2F and Sp 1 DNA binding sites and was inhibited by dominant negative E2F 1 or DP 1 . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Regulation of the human cyclin dependent kinase inhibitor p18INK4c by the transcription factors E2F1 and Sp 1 . ^^^ Mutational inactivation of these elements revealed that the Sp 1 sites were important for the basal activity of the promoter but could also mediate the effects of E2F1 on the p 18 promoter . ^^^ Moreover , we found that E2F1 and Sp 1 can synergistically enhance the activity of the proximal p 18 promoter . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Analysis of the hMT1G promoter showed the presence of many potential E2F binding sites flanked by potential SP 1 sites and metal response elements ( MREs ) . hMT1G promoter could be induced by E2F1 in transient transfections ; further , deletion analysis suggested that the region spanning the E2F binding sites was necessary for VEGF mediated induction . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Analysis of the promoter region of fTERT showed the presence of several transcription factor binding sites ( E2F 1 , E box , ER , Sp 1 and USF sites ) in common with the hTERT promoter , and whose binding factors are known to regulate hTERT . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Expression of factors regulating Apaf 1 transcription , such as E2F 1 , p 53 , and Sp 1 , did not differ between Apaf 1 positive and Apaf 1 negative cells . ^^^ |
|
| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| Subsequent mutation / deletion analysis showed that at least three different cis elements were involved in activation of cdc25A by E 2 , namely , GC rich Sp 1 binding sites , CCAAT motifs that bind NF Y , and E2F sites that bind DP / E2F1 proteins . ^^^ |
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| Interacting proteins: Q01094 and P08047 |
Pubmed |
SVM Score :0.0 |
| We found that the minimum VEGF promoter mediating transcriptional repression by E2F1 features an E2F1 binding site with four Sp 1 sites in close proximity . ^^^ |
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