Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.92498317
We also demonstrate that E2F 1 becomes transcriptionally active yet remains associated with Rb . 0.92498317^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.64588544
However , that of E2F 1 was negatively correlated with Rb . 0.64588544^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Conversely , E2F 1 overrode an RB induced G 1 block more efficiently than E2F 4 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
However , coexpression of Rb suppressed E2F 1 induced apoptosis in HuH 7 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Also like E1A , RIZ can form a ternary complex with Rb and E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.93430651
These data , together with the fact that RB binds to E2F 1 in vivo , suggest that E2F 1 is a downstream target of RB action . 0.93430651^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.60111386
E2F 1 forms a heterodimer with DP 1 and binds to several cell cycle regulatory proteins , including the retinoblastoma family ( RB , p 107 , p 130 ) and cyclin A / CDK2 complexes . 0.60111386^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.69202307
We have studied the interaction of E2F 1 with the Rb and adenovirus E 4 proteins and show that phosphorylation of E2F 1 on serine residues 332 and 337 prevented its interaction with Rb but was a prerequisite for interaction with E 4 . 0.69202307^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.74116756
These studies show that Rb interaction with E2F1 is regulated by cdk 4 and cyclin D 1 within p 210 BCR ABL transformed leukemia cells in early G 1 phase of the cell cycle . 0.74116756^^^ This interaction between Rb and E2F1 , which is subject to the level of cAMP , is critical to transcriptional control of c myc . 0.692399^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.9477535
We propose that competition between Rb and both TBP and TFIIH for binding to E2F1 is a mechanism by which Rb inhibits transactivation by E2F1 . . 0.9477535^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , DDB expression could overcome the retinoblastoma protein ( Rb ) mediated inhibition of E2F1 activated transcription . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , the temporal kinetics of R Rb interaction correlate with displacement of E2F1 from Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
This induction of E2F 1 occurs independently of Rb or p 53 status and involves new protein synthesis . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E2F 1 protein is able to induce generalized apoptosis in gliomas independently of the p 53 , p 16 or Rb status . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Interaction of specific cellular proteins , including E2F1 , with Rb C terminal domains mediates cell cycle regulation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The transcription factor E2F 1 is regulated by Rb and is reported to evoke death in proliferating cells when overexpressed . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Among these is the transcription factor E2F1 , a final effector of the RB pathway , that induces ARF expression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Levels of E2F 1 bound to Rb also were elevated . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Western blotting showed that underphosphorylated forms of RB 1 were elicited by RB1CC1 , whereas E2F1 was not affected . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In contrast to RB , E2F1 consistently maintained a stable association with E2F sites regardless of cell type . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
RB function was assessed through interaction with E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
No mutations in either RB 1 or the Rb binding pocket of E2F 1 were detected . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E2F1 transcription factor is a critical downstream target of the tumor suppressor RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Finally , p 300 induced E2F 1 ubiquitination is not influenced by RB . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Structure of the Rb C terminal domain bound to E2F1 DP 1 : a mechanism for phosphorylation induced E2F release . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
This suggests that E2F1 could be a substrate for Hsc 70 recruited by T antigen to an Rb family member . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Phosphorylation of serines 608 / 612 inhibits binding of E2F 1 to RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , the RB gene product copurifies with E2F 1 activity . ^^^ Taken together , we conclude that the product of the RB gene is a part of E2F 1 and is involved in the regulation of E2F activity . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Regulation of E2F 1 gene expression by p 130 ( Rb 2 ) and D type cyclin kinase activity . ^^^ Regulation of E2F 1 is accomplished through at least two mechanisms : posttranslational regulation by binding proteins such as Rb and transcriptional regulation of the E2F 1 gene . ^^^ We now demonstrate that the presence of an E2F DNA binding complex containing the Rb related p 130 protein ( Rb 2 ) correlates with E2F 1 gene repression and that overexpression of p 130 inhibits transcription from the E2F 1 promoter . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , the cyclin E gene is strongly induced by E2F1 , thus defining an autoregulatory circuit since cyclin E dependent kinase activity can stimulate E2F1 transcription , likely through the phosphorylation and inactivation of Rb and Rb family members . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Expression of Rb , E2F1 , cdc 2 , and D , and B cyclins in developing spinal cord . ^^^ The spatial and temporal distribution of transcripts for the tumor suppressor gene Rb , transcription factor E2F1 , cdc 2 kinase , cyclins D 1 , D 2 , B 1 and B 2 during neurogenesis of the spinal cord was determined by in situ hybridization . ^^^ The Rb and E2F1 transcripts were detectable in proliferating and differentiating cells . ^^^ These observations suggest that Rb and E2F1 may be involved in the early stages of neuronal differentiation in addition to the cell cycle regulation . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
RB and a novel E2F 1 binding protein in MHC class 2 deficient B cell lines and normal IFN gamma induction of the class IL transactivator CIITA in class 2 non inducible RB defective tumor lines . ^^^ Retinoblastoma protein ( RB ) is a tumor suppressor and functions as a transcriptional repressor by binding and inactivating the transactivator E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using expression vectors carrying mutated Rb cDNAs , we have identified two portions of Rb required for superactivation : ( 1 ) a portion of the Rb `` pocket ' ' ( amino acids 614 839 ) previously determined to be required for physical interactions between Rb and transcription factors such as E2F 1 and ( 2 ) a novel amino terminal region ( amino acids 140 202 ) . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
However , Rb also binds to H NUC at the tetratricopeptide repeat motif , a region which contains sequences different from the binding motifs of either T antigen or E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In addition , an E2F1 chimeric protein , in which sequences involved in Rb binding have been replaced with the herpesvirus VP 16 activation domain , exhibits increased transformation activity . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Changing the tumor suppressor function of Rb by expressing transcription factor E2F1 and viral oncoprotein E1A in cerebellar granular neurons in vitro and in cerebral cortical neurons in vivo results in the induction of DNA synthesis in these neurons . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Interestingly , the binding of E 4 6 / 7 and the binding of Rb to E2F are mutually exclusive , and our results show that the same internal segments of E2F 1 and DP 1 that are required for E 4 6 / 7 binding are also required for stable interaction with Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Regulation of adenovirus 12 E1A transcription : E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1 , DP 1 , cyclin A and / or RB and mediate transcriptional ( auto ) activation . ^^^ For HeLa , 293 , U 937 , and A 549 cells , participation of E2F 1 , DP 1 , cyclin A , and RB was involved in formation of some complexes only , assuming participation of factors different from E2F 1 or DP 1 in others . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We now find that the E2F1 gene , which encodes one of the components of E2F activity , is subject to autoregulatory control during progression from G 0 to S phase and that this primarily reflects a negative control in G 0 and early G 1 , a time when the majority of E2F activity exits as a complex with Rb family members . ^^^ We conclude that the E2F1 gene is a downstream target for G 1 cyclin dependent kinase activity , most likely as a consequence of phosphorylation of Rb family members , and that the autoregulation of E2F1 transcription may provide a sensitive switch for regulating the accumulation of E2F activity during the transition from G 1 to S phase . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
While the three cloned species of E2F , E2F 1 , E2F 2 , and E2F 3 , are known to be targets of RB interaction , no E2F species has yet been shown to be a specific p 107 or p 130 target . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F 1 is a transcription factor suspected of activating genes required for S phase and a known target for the action of RB , the retinoblastoma gene product . ^^^ E2F 1 mediated apoptosis was suppressed by coexpression of wild type RB or a transdominant negative mutant species of p 53 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Thus , specific and timely cell cycle dependent interactions of E2F 1 with proteins that inhibit its function ( i . e . , RB during G 1 and cyclin A kinase during S / G2 ) may contribute to the periodicity of expression of certain E2F 1 responsive genes at the G1 / S transition . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The transformation caused by E2F 1 correlates with constitutive overexpression of the transgene , increased transcription of E2F dependent genes and the enhancement of two E2F DNA binding complexes containing the retinoblastoma susceptibility gene product ( Rb ) and E2F 1 . ^^^ The oncogenic potential of E2F 1 is dependent on functional DNA binding and transactivation domains but does not require the ability to interact directly with Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Although HPV 1 E7 showed the same affinity in vitro and in vivo for Rb 1 as the high risk HPV E7s , it did not have the ability to activate the E2F 1 transcription factor inhibited by Rb 1 , nor did it have any transforming activity when coexpressed with activated ras in primary rodent cells . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E2F 1 transcription factor is a protein partner of the retinoblastoma susceptibility gene product , RB . ^^^ Thus , the cell cycle utilizes an interacting or communicative pathway between RB E2F 1 and p53 . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using CAT reporter co transfection assays we show here that transcription from the MYC P 2 promoter is induced efficiently by E2F 1 , but repressed by RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The transcription factor E2F 1 mediates the autoregulation of RB gene expression . ^^^ By using the cloned E2F 1 gene , here we report that ( 1 ) RB expression is negatively regulated by its own gene product , ( 2 ) E2F 1 binds specifically to an E2F recognition sequence in the RB promoter and transactivates the RB promoter , ( 3 ) overexpression of RB suppresses E2F 1 mediated stimulation of RB promoter activity , and ( 4 ) the expression of the RB gene is paralleled by the expression of the E2F 1 gene during cell cycle progression . ^^^ These results demonstrate that expression of RB is negatively autoregulated through E2F 1 . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
This region also contains an 18 residue retinoblastoma ( RB ) binding sequence , raising the possibility that RB binding might inhibit the ability of E2F 1 to form protein protein contacts required for activation . ^^^ In addition , we show that overexpression of RB , but not the RB mutant , RBd 22 , can inhibit GAL4 / E2F 1 activity in vivo . ^^^ Three different GAL4 / E2F 1 mutants that activate transcription , but fail to bind to RB , are not significantly affected by overexpression of RB . ^^^ These findings support a model wherein RB suppresses E2F 1 mediated transcriptional activation through direct physical association . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
A genetic analysis of the E2F1 gene distinguishes regulation by Rb , p 107 , and adenovirus E 4 . ^^^ The recent isolation of the E2F1 cDNA clone , which encodes a polypeptide with properties characteristic of E2F , has now allowed a more detailed analysis of the regulation of E2F function by Rb as well as the Rb related p 107 protein and the adenovirus 19 kDa E 4 gene product . ^^^ In contrast , the leucine repeat element was not important for Rb or p 107 mediated inhibition of E2F1 activity . ^^^ Although the C terminal acidic domain of E2F1 , previously shown to be important for Rb binding , appears to be a site for regulation of E2F1 by Rb and p 107 , point mutations within this region distinguish recognition by Rb and p 107 . ^^^ These results underscore the complexity of E2F regulatory interactions and also demonstrate a qualitative distinction in the interactions of Rb and p 107 with E2F1 , perhaps reflective of functional differences . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Here we show that a chimera in which the E2F1 transactivation domain was replaced with the RB pocket could , in a DNA binding and pocket dependent manner , mimic the ability of RB to repress transcription and induce a cell cycle arrest . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Disruption of RB / E2F 1 interaction by single point mutations in E2F 1 enhances S phase entry and apoptosis . ^^^ To study the biological consequences of the RB / E2F 1 interaction , point mutants of E2F 1 which fail to bind to RB were isolated by using the yeast two hybrid system . ^^^ Sequence analysis revealed that within the minimal 18 amino acid peptide of E2F 1 required for RB binding , five residues , Tyr ( position 411 ) , Glu ( 419 ) , and Asp Leu Phe ( 423 425 ) , are critical . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
It is likely that binding of growth suppressing proteins , such as Rb , can inhibit the transactivation potential of E2F1 , either by blocking the interaction of E2F1 with a separate component of the transcription complex or by bringing a repressor domain to the transcription complex ( Flemington et al . 1993 ; Helin et al . 1993 ; Weintraub et al . 1992 ; Zamanian and La Thangue 1993 ; Zhu et al . 1993 ) . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , the cells were protected from IL 1 inducible growth inhibition by ectopic expression of dominant negative mutants of the Rb gene , or the transcription factor E2F 1 , which is a downstream target of RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Overexpression of the human retinoblastoma suppressor gene product RB suppressed E2F 1 mediated transcriptional activation , indicating an E2F dependent regulation of promoter activity during the G 1 to S phase transition . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F1 and E1A ( 12S ) have a homologous activation domain regulated by RB and CBP . ^^^ The sequence homology reflects , at least partly , the conservation of common binding sites for the RB and CBP / p300 proteins , which are preserved in the same relative order along E2F1 and E1A . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , co transfection with the RB , which inhibits the transcription activity of E2F1 , can also repress E2F1 inhibition of myoD transactivation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In addition , TGF beta 1 was also found to increase E2F 1 protein levels but had no effect on steady state retinoblastoma ( RB ) protein levels or phosphorylation state . ^^^ In the absence of TGF beta 1 , transient expression of RB in 5 Abl expressing cells resulted in decreased c myc transcription , inhibition of GAL 4 E2F 1 driven transactivation and inhibition of cellular proliferation . ^^^ In summary , these studies established that in myeloid cells : ( 1 ) 5 Abl binds RB resulting in increased E2F 1 driven c myc transcription , and ( 2 ) an alternative pathway exists for TGF beta 1 mediated growth inhibition of 5 Abl transformed cells , in which increased rather than decreased E2F mediated c myc transcription is observed . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Downstream targets of Rb , such as the E2F1 transcription factor , can promote cell cycle progression , whereas inhibitors of CDK 4 and CDK 6 , such as p16CDKN2a , can block G 1 progression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
During most of the G 1 phase , E2F1 / DP1 activity is repressed by the retinoblastoma gene product RB , which directly contacts the E2F1 activation domain and silences it . ^^^ The E2F1 activation domain has sequence similarity to the N terminal activation domain of E1A ( 12S ) , which contains binding sites for CBP as well as RB . ^^^ Deletion of four residues from the E2F1 activation domain reduces CBP binding as well as transcriptional activation , but still allows the binding of RB and MDM 2 . ^^^ The convergence of the RB and CBP pathways on the regulation of E2F1 activity may explain the cooperativity displayed by these proteins in mediating the biological functions of E1A . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Such a role contrasts with the function of Rb in arresting cell cycle progression in G 1 by negative regulation of other transcription factors like E2F 1 . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Cleaved Rb bound cyclin D 3 and inhibited the transcriptional activity of E2F 1 , but failed to bind to the regulatory protein MDM 2 , which has been implicated in apoptosis . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The functions of the transcription factor E2F 1 are regulated by the RB protein through the RB binding domain of E2F 1 and this factor is considered to be an important molecule that functions downstream of the RB protein . ^^^ In order to determine whether E2F 1 that can not bind to RB might be associated with various human cancers , we searched for mutations in the RB binding domain of E2F 1 using samples of DNA from various clinical specimens obtained from 406 cancer patients ( with lung , pancreatic , stomach , colon , esophageal , and hepatic cancers ) by analysis of polymerase chain reaction mediated single strand conformational polymorphism . ^^^ These results suggest that a mutation or deletion in E2F 1 that might affect binding of the RB protein is not involved in human cancers . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The effects of ZD 1694 on the expression of E2F1 and formation of the E2F1 Rb complex were investigated after a 2 hr drug exposure ( IC 90 ) . ^^^ The results showed a time dependent decrease in E2F1 mRNA and protein expression ; an increase in the abundance of the E2F Rb complex could be demonstrated beginning 4 hr after drug exposure by a gel shift assay . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In contrast , IFN alpha did not affect the DNA binding ability of E2F 1 and E2F 4 in a complex with retinoblastoma ( RB ) susceptibility gene family proteins including pRB , p 107 , and p 130 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Formation of the early region 2 transcription factor 1 retinoblastoma protein ( E2F 1 RB ) transrepressor and release of the retinoblastoma protein from nuclear complexes containing cyclin A is induced by interferon alpha in U937V cells but not in interferon alpha resistant U937VR cells . ^^^ In U937V cells IFN alpha induced the formation of the complex between early region 2 transcription factor 1 ( E2F 1 ) and retinoblastoma protein ( RB ) which is known to repress transcription of E2F 1 inducible genes , necessary for cell cycle progression . ^^^ Formation of this complex was not inducible by IFN alpha in U937VR cells , although the suitable binding partners ( E2F 1 and under phosphorylated RB ) were present . ^^^ Interestingly , treatment of nuclear extracts from logarithmically growing U937V and U937VR cells with an antiserum against cyclin A that disrupts cyclin A containing complexes , led to the formation of the E2F 1 RB complex , suggesting the presence of under phosphorylated ( active ) RB , trapped in nuclear complexes that contain cyclin A . ^^^ We conclude that the missing induction of the E2F 1 RB transrepressor by IFN alpha and the failure to release RB from cyclin A containing complexes might contribute to the resistance of U937VR cells to the antiproliferative effects of IFN alpha . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using Saos 2 cells , which are null for p 53 and lack functional Rb , we have demonstrated that E2F1 overexpression can also induce apoptosis in the absence of p 53 and retinoblastoma protein ( Rb ) . ^^^ E2F1 induced apoptosis can be specifically inhibited by Rb but not mdm 2 , which is known for its ability to inhibit p 53 induced apoptosis . ^^^ The transactivation defective E2F1 mutants E2F1 ( 1 374 ) , E2F1 ( 390 1 ) DF ( delta mdm 2 ) , and E2F1 ( 406 415 ) ( delta Rb ) can induce apoptosis as effectively as wild type E2F1 . ^^^ Therefore Rb may inhibit E2F1 induced apoptosis by mechanisms other than the suppression of the transactivation of E2F1 . ^^^ This hypothesis was supported by our observation that although Rb overexpression can specifically repress the apoptosis induced by wild type E2F1 and a Rb binding competent E2F1 mutant E2F1 ( 390 1 ) DF ( delta mdm 2 ) , it failed to inhibit the apoptosis induced by mutants E2F1 ( 1 374 ) and E2F1 ( delta 406 415 ) ( delta Rb ) , which are defective or reduced in Rb binding and transactivation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The molecular mechanisms involved in these roles have been elucidated in some biological systems : Rb sequesters the transcription factors of E2F 1 to regulate entry of cell cycle , but enhances the activities of transcription factors such as C / EBPs to initiate terminal differentiation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
There is also recent evidence which suggests that mdm 2 may play roles in p 53 independent pathways regulating cellular proliferation . mdm 2 has recently been shown to interact with the retinoblastoma tumor suppressor protein p ( Rb ) , and the E2F 1 and DP 1 transcription factors . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
RB and hbrm cooperate to repress the activation functions of E2F1 . ^^^ A major target of the RB protein is the S phase inducing transcription factor E2F1 . ^^^ RB binds directly to the activation domain of E2F1 and silences it , thereby preventing cells from entering S phase . ^^^ We show here that in transfected cells RB can contact both E2F1 and hbrm at the same time , thereby targeting hbrm to E2F1 . ^^^ Furthermore , RB and hbrm cooperate to repress E2F1 activity in transient transfection assays . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In contrast , two low penetrant alleles ( 661W and `` deletion of codon 480 ' ' ) retained the ability to localize to the nucleus , showed normal cdk mediated hyperphosphorylation in vivo , exhibited a binding pattern to simian virus 40 large T antigen using a quantitative yeast two hybrid assay that was intermediate between classic mutants ( null ) and wild type RB , and had absent E2F1 binding in vitro . ^^^ A third , low penetrant allele , `` deletion of RB exon 4 , ' ' showed minimal hyperphosphorylation in vivo but demonstrated detectable E2F1 binding in vitro . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The effects of E 2 on the major cyclins , cyclin dependent kinases and cyclin dependent kinase inhibitors , retinoblastoma protein ( RB ) , E2F 1 , and cyclin dependent kinase activities were also investigated in the stably transfected MDA MB 468 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
However , the nuclear binding partner of the p 68 RB fragment is not the transcription factor E2F 1 since a specific E2F 1 antibody coimmunoprecipitated only the unphosphorylated form of RB , but not the p 68 fragment . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Some of these extracellular stimuli arrest cells in the G 1 phase of the mitotic division cycle and modulate the activity of the tumor suppressor protein Rb and its partner E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using recombinant E2F , DP , and Rb proteins prepared in baculovirus infected cells and a repetitive immunoprecipitation PCR procedure ( CASTing ) , we have identified consensus DNA binding sites for E2F 1 / DP 1 , E2F 1 / DP 2 , E2F 4 / DP 1 , and E2F 4 / DP 2 complexes as well as an Rb / E2F 1 / DP 1 trimeric complex . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using adenoviral E1A , we previously demonstrated dual pocket protein and p 300 dependent pathways in neonatal rat cardiac myocytes , and have proven that E2F 1 , which occupies the Rb pocket , suffices for these actions of E1A . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We then examined the ability of necdin to bind to the transcription factor E2F1 , a cellular Rb binding factor involved in cell cycle progression . ^^^ Intriguingly , necdin , like Rb , bound to a carboxyl terminal domain of E2F1 , and repressed E2F dependent transactivation in vivo . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Loss of E2F 1 reduces tumorigenesis and extends the lifespan of Rb 1 ( + / ) mice . ^^^ To determine whether E2F 1 deregulation as a result of loss of pRB promotes proliferation in vivo , we have tested whether loss of E2f1 interferes with the pituitary and thyroid tumorigenesis that occurs in Rb 1 ( + / ) mice . ^^^ We have found that loss of E2f1 reduces the frequency of pituitary and thyroid tumours , and greatly lengthens the lifespan of Rb 1 ( + / ) ; E2f1 ( / ) animals , demonstrating that E2F 1 is an important downstream target of pRB during tumorigenesis . ^^^ Furthermore , loss of E2f1 reduces a previously reported strain dependent difference in Rb 1 ( + / ) lifespan , suggesting that E2f1 or an E2F 1 regulated gene acts as a genetic modifier between the 129 / Sv and C57BL / 6 strains . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In contrast , inhibition of RB binding to E2F or ectopic expression of E2F 1 in plaque VSMCs induced massive apoptosis , which required suppression of p 53 to rescue cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Coexpression of a cyclin D 1 transgene with E2F1 augments epidermal hyperplasia and further disrupts hair follicle development suggesting that hypophosphorylated Rb antagonizes the proliferative and apoptotic promoting activities of E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also detected higher levels of Rb phosphorylation and E2F 1 protein levels in LNCaP / cyclin D 1 cells than that in the parental and vector control cells in medium with or without androgen . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , enforced overexpression of the DNA binding defective E2F1E132 mutant conferred TPA independence upon melanocytes and was associated with sequestration of Rb and constitutive expression of E2F1 target genes , including p21WAF1 / CIP1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Clinical implications of cyclins , cyclin dependent kinases , RB and E2F1 in squamous cell lung carcinoma . ^^^ In the search for new risk factors at the molecular and cellular levels , clinical data [ lymph node involvement ( LN ) and stage ] were used and 104 squamous cell lung carcinomas were analyzed by immuno histochemistry for expression of cyclin D 1 , cyclin A , cdk 2 , cdk 4 , RB , and E2F1 . ^^^ The results of the univariate analysis of all 8 factors showed that cyclin A and cdk 2 gave the best prognostic information , while no prognostic value could be found associated with cyclin D 1 , cdk 4 , RB and E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
To test this hypothesis , a recently developed quantitative reverse transcriptase polymerase chain reaction method was used to assess simultaneously expression of 15 genes mechanistically associated with cell cycle control ( c myc , E2F 1 , p 21 , rb , PCNA , cyclin D 2 , cyclin D 3 , cyclin E , cdc 2 , CDK 2 , CDK 4 , mad , max p 21 , max p 22 , and p 53 ) in normal cell cultures from five individuals and in nine different malignant BEC lines . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The S phase inhibitory activity of PSM RB could be attenuated by the coinjection of SV 40 T antigen , adenovirus E1A , or a high level of E2F 1 expression plasmids . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In vivo , Bog / Rb complexes do not contain E2F 1 , and Bog can displace E2F 1 from E2F 1 / Rb complexes in vitro . ^^^ These data suggest that Bog may be important in the transformation process , in part due to its capacity to confer resistance to the growth inhibitory effects of TGF beta 1 through interaction with Rb and the subsequent displacement of E2F 1 . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Interaction of E2F / Rb family members with factor binding to co repressor element on B myb and E2F1 promoters ] . ^^^ The transcription of a number of these genes , including E2F1 and B myb , is repressed in G0 / early G 1 at E2F DNA binding sites mediated by interaction of E2F with the Rb family member proteins . ^^^ The CHR mutated promoter is activated by the E2F family of proteins ( E2F1 , E2F2 , E2F3 , and E2F4 ) but unable to be repressed by any of the Rb family members ( Rb , p 107 , and p 130 ) to the level of the wild type promoter activity in G 0 , indicating that the repression by the Rb family members is required for the corepressor element . ^^^ These results seggested that E2F and the Rb family member proteins regulate the transcription of the E2F1 and B myb genes by associating with an additional corepressor protein . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We have previously shown that Rb , the founding member of the pocket proteins family , represses E2F1 activity by recruiting the histone deacetylase HDAC 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , overexpression of the E2F 1 transcription factor , a downstream target of Rb , induced extensive apoptosis and IL 1alpha release . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Mutation of E2f 1 suppresses apoptosis and inappropriate S phase entry and extends survival of Rb deficient mouse embryos . ^^^ We show here that embryos mutant for both Rb and its downstream target E2f 1 demonstrate significant suppression of apoptosis and S phase entry in certain tissues compared to Rb mutants , implicating E2f 1 as a critical mediator of these effects . ^^^ Up regulation of the p 53 pathway , required for cell death in these cells in Rb mutants , is also suppressed in the Rb / E2f 1 double mutants . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
To analyze its role as a promoter of cell growth arrest during development , we have studied the temporal pattern of Rb expression and its association with E2F 1 during embryogenesis of the quail neuroretina . ^^^ Moreover , cell cycle exit at E 6 E7 is characterized by a sudden and transient rise of the E2F 1 / RB complex followed by the appearance of the E2F 4 / p130 complex starting at E 8 . ^^^ Conversely , expression of adenovirus E1A protein in E 6 neuroretina cells leads to a dissociation of E2F 1 / Rb complex and suppression of cell growth arrest and differentiation . ^^^ This suggests that cell cycle exit and re entry may depend on Rb / E2F 1 interaction . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Raf 1 can inactivate Rb function and can reverse Rb mediated repression of E2F1 transcription and cell proliferation efficiently . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Overexpression of Rb and E2F 1 in ataxia telangiectasia lymphocytes . ^^^ Exponentially growing normal and AT cells were exposed to 2 Gy of ionizing radiation and the expression levels and functional activities of Rb and E2F 1 proteins were determined . ^^^ We observed overexpression of hyperphosphorylated Rb and E2F 1 proteins in AT cells , which was unaffected post irradiation . ^^^ The data suggests that abnormalities in the function of Rb and E2F 1 proteins may also be responsible for the failure of AT cells to arrest in the G1 / S checkpoint in response to DNA damage . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Functions of cyclin A 1 in the cell cycle and its interactions with transcription factor E2F 1 and the Rb family of proteins . ^^^ We report that cyclin A 1 could bind to important cell cycle regulators : the Rb family of proteins , the transcription factor E2F 1 , and the p 21 family of proteins . ^^^ Associations of cyclin A 1 with Rb and E2F 1 were observed in vivo in several cell lines . ^^^ When cyclin A 1 was coexpressed with CDK 2 in sf 9 insect cells , the CDK 2 cyclin A 1 complex had kinase activities for histone H 1 , E2F 1 , and the Rb family of proteins . ^^^ Our results suggest that the Rb family of proteins and E2F 1 may be important targets for phosphorylation by the cyclin A 1 associated kinase . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Neural precursor cells differentiating in the absence of Rb exhibit delayed terminal mitosis and deregulated E2F 1 and 3 activity . ^^^ Rb deficient cells exhibit a significant enhancement of E2F 1 and 3 activity throughout differentiation concomitant with the aberrant expression of E2F inducible genes . ^^^ In these studies we show that Rb is essential for the regulation of E2F 1 and 3 activity as well as the onset of terminal mitosis in neural precursor cells . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Rb and E2f 1 in mouse development and tumorigenesis . ^^^ Our understanding of how RB and E2F 1 function has progressed significantly from the model in which RB negatively regulates expression of genes required for S phase by binding to and inhibiting E2F 1 . ^^^ Both RB and E2F 1 have been shown recently to possess additional properties and mechanisms of regulation relevant to developmental and tumorigenic processes . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Here we report that two kinases involved in signal transduction have opposite effects on E2F function : the stress induced kinase JNK 1 inhibits E2F1 activity whereas the related p 38 kinase reverses Rb mediated repression of E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Consistent with this , overexpression of E2F 1 , whose function is repressed by the hypophosphorylated form of Rb , also rescued the cell cycle arrest . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F 1 is an RB associated transcriptional factor and an effector of RB in the regulation of G 1 to S phase transition . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Rb binding by E2F 1 was neither necessary nor sufficient for G 1 exit , whereas DNA binding was required ; thus , exogenous E2F 1 did not merely function by competing for the Rb `` pocket . ' ' E2F 1 induced G 1 exit was blocked by the `` universal ' ' Cdk inhibitor p 21 but not by p 16 , a specific inhibitor of Cdk4 / 6 ; p 21 was permissive for E2F 1 induction of cyclins E and A , but prevented their stimulation of Cdk 2 kinase activity . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F 1 , a transcription factor implicated in the activation of genes required for S phase such as DNA pol alpha , is regulated by interactions with Rb and by cell cycle dependent alterations in E2F 1 abundance . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Rb and prohibitin target distinct regions of E2F1 for repression and respond to different upstream signals . ^^^ Agents such as E1A , p 38 kinase , and cyclins D and E had no effect on prohibitin mediated repression of E2F1 , but all of these molecules could reverse Rb function . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Gankyrin bound to the product of the retinoblastoma gene ( RB 1 ) , increasing its phosphorylation and releasing the activity of the transcription factor E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The Brca 1 promoter contains E2F DNA binding sites that mediate transcriptional activation by E2F1 and repression by Rb . ^^^ The human BRCA 1 promoter also contains a conserved E2F site and is similarly regulated by E2F1 and Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E1A proteins displace repressors of E2F activity ( the Rb family members ) and thus relieve E2F 1 promoter repression ; the E 4 6 / 7 protein complements this function by stably recruiting active E2F to the E2F 1 promoter to transactivate expression . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Given that E2F1 activity is stimulated by p300 / CBP acetylase and repressed by an RB associated deacetylase , we asked if E2F1 was subject to modification by acetylation . ^^^ These results suggest that acetylation stimulates the functions of the non RB bound ' free ' form of E2F1 . ^^^ Consistent with this , we find that the RB associated histone deacetylase can deacetylate E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Studies evaluating alterations of the retinoblastoma pathway have identified the retinoblastoma gene , RB , p161NK4A / CDKN2 , and E2F 1 as tumor suppressor genes with potential prognostic significance in patients with bladder cancer . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Finally , we found that E2F1 and RbAp 48 are physically associated in the presence of Rb and HDAC 1 , suggesting that RbAp 48 could be involved in transcriptional repression of E2F responsive genes . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Functionally , we demonstrate that Che 1 represses the growth suppression function of Rb , counteracting the inhibitory action of Rb on the trans activation function of E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Frequent disruption of the RB 1 pathway in diffuse large B cell lymphoma : prognostic significance of E2F 1 and p16INK4A . ^^^ In the present study , we analysed 34 de novo diffuse large B cell lymphoma ( DLCL ) from a population based lymphoma registry for alterations of the RB 1 pathway at the genetic ( RB 1 and CDK 4 ) and protein ( pRb , cyclin D 1 , cyclin D 3 , CDK 4 , and E2F 1 ) level . ^^^ These data support a role of E2F 1 as tumour suppressor gene in lymphoma and strongly suggest that the RB 1 and p 53 pathways are important in the development of de novo DLCL . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Stress signals induce transcriptionally inactive E2F 1 independently of p 53 and Rb . ^^^ However , stress signals can induce E2F 1 expression independently of p 53 and Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Two separate sets of primers were used that recognized the regulatory retinoblastoma ( Rb ) protein binding region and the functional DNA binding region of E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We further show that the dominant negative RB inhibits E2F1 but can not inhibit a forced E2F1 RB dimer . ^^^ Therefore , E2F1 is a potential mediator of the dominant negative inhibition of MyoD by CB RB during satellite cell differentiation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
DNMT 1 forms a complex with Rb , E2F1 and HDAC 1 and represses transcription from E2F responsive promoters . ^^^ Here we show that the predominant mammalian DNA methyltransferase , DNMT 1 , co purifies with the retinoblastoma ( Rb ) tumour suppressor gene product , E2F1 , and HDAC 1 and that DNMT 1 cooperates with Rb to repress transcription from promoters containing E2F binding sites . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Prolonged treatment with MIS down regulated the Rb related protein p 130 and increased the Rb family regulated transcription factor E2F1 , overexpression of which inhibited growth . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The expression of bcl 2 , RB , and E2F 1 was studied using Western blot analysis . ^^^ Furthermore , we demonstrated that these changes were accompanied by cell cycle arrest , up regulation of RB , and down regulation of E2F 1 and bcl 2 proteins . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Electrophoretic mobility shift assays and immunoprecipitation experiments demonstrated that the complexes formed by E2F1 and E2F4 and Rb family proteins vary throughout in vitro keratinocyte differentiation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The absence of p 53 , Rb , cyclin D 1 , or E2F1 does not affect the apoptotic effect of overexpressed beta catenin , but Bcl 10 ( L ) reduces it . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F1 and p 53 are dispensable , whereas p 21 ( Waf1 / Cip1 ) cooperates with Rb to restrict endoreduplication and apoptosis during skeletal myogenesis . ^^^ Although E2F1 and p 53 mediate ectopic DNA synthesis and cell death in several tissues in Rb mutant embryos , both endoreduplication and apoptosis persisted in mgRb : Rb / : E2F1 / and mgRb : Rb / : p 53 / compound mutant muscles . ^^^ Thus , combined inactivation of Rb and p 21 ( Waf1 / Cip1 ) augments endoreduplication and apoptosis , whereas E2F1 and p 53 are dispensable during aberrant myogenesis in Rb deficient fetuses . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
TSA enhanced the protein expression of p 21 ( WAF 1 ) , CREB binding protein , cyclinE , cyclin A , Bak and Bax , while it reduced the expression of E2F 1 , E2F 4 , HDAC 1 , p 53 and hyperphosphorylated form of Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F1 mediates ectopic proliferation and stage specific p 53 dependent apoptosis but not aberrant differentiation in the ocular lens of Rb deficient fetuses . ^^^ Mutant mouse embryos lacking Rb exhibit ectopic proliferation and apoptosis that are mediated in some tissues by E2F1 , a major partner of Rb , and by the p 53 tumor suppressor . ^^^ Whether E2F1 and p 53 also mediate the differentiation defects in Rb mutant embryos is , however , not clear . ^^^ The abnormal cell proliferation and apoptosis were significantly suppressed in the lens of compound mutant fetuses lacking both Rb and E2F1 at embryonic day ( E ) E15 . 5 . ^^^ Interestingly however , at E18 . 5 , only ectopic proliferation , not apoptosis , was dramatically reduced in mgRb : Rb / : E2F1 / lenses . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Hepatitis B viral 10 protein overcomes inhibition of E2F1 activity by pRb on the human Rb gene promoter . ^^^ Interestingly , HBx transactivated the Rb promoter cooperatively with E2F1 in HepG 2 cells but not in HeLa cells , in which the functions of p 53 and pRb are inactive . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In vitro , E2F1 can be ubiquitinated by E2 / Ubc5 but not by E2 / CDC34 , is dependent on catalytically active ROC 1 , and is protected by the Rb protein . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The overexpression of Ebp 1 in MCF 7 and AU 565 ( Rb ( + ) ) cells inhibited the activity of the E2F1 regulated cyclin E promoter . ^^^ Ebp 1 bound E2F1 indirectly via Rb in lysates of MCF 7 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also found coinduction of several groups of related genes that might represent functional modules within the ischemic neuronal transcriptome , including VEGF and its receptor , NRP 1 ; the IGF 1 receptor and the IGF 1 binding protein IGFBP 2 ; Rb , the Rb binding protein E2F1 , and the E2F related transcription factor , TFDP 1 ; the CACNB 3 and CACNB 4 beta subunits of the voltage gated calcium channel ; and caspase 3 and its substrates , ACINUS , FEM 1 , and GSN . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The latter event is consistent with a role for the Rb regulated E2F1 protein as a specific inducer of apoptosis and p 53 accumulation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also found that Suv39H1 and Rb cooperate to repress E2F activity and that Suv39H1 could be recruited to E2F1 through its interaction with Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Analyses of the E2F1 promoter activity , in normal and Rb deficient fibroblasts converted by MyoD , identified a MyoD function stimulating E2F1 expression . ^^^ The E2F1 induction was very manifest in the Rb / cells , but also detectable , at the early stage of differentiation , in normal cells . ^^^ Its effects , although not indispensable for myogenesis , presumably contribute to raise the concentration of Rb E2F1 transcription repressing complexes , since MyoD strongly induces also Rb in differentiating myocytes . ^^^ In the absence of Rb , however , the induced E2F1 is left with only its activating role , reversing the normal effect of this MyoD function . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F1 activity is negatively regulated by the retinoblastoma ( RB ) protein . ^^^ To study how inactivation of Rb and dysregulated E2F1 affects the developing retina , we analysed wild type and Rb ( / ) embryonic retinas and retinal transplants and we established transgenic mice expressing human E2F1 in retinal photoreceptor cells under the regulation of the IRBP promoter ( TgIRBPE2F1 ) . ^^^ Both RB deficient and E2F1 overexpressing photoreceptor cells expressed rhodopsin , a marker of terminal differentiation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Our results indicate that Apaf 1 plays a critical role in apoptosis in a subset of tissues and that both E2F1 : p 53 : Apaf 1 dependent and independent apoptotic pathways operate downstream of Rb . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E2F1 transcription factor is a critical downstream target of the Rb tumor suppressor and , when active , can promote proliferation as well as apoptosis . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , results obtained with Rb deficient cell lines indicated that the reduced E2F 1 expression is independent of pRb . ^^^ These results suggest that Ad vector induced cell cycle dysregulation is associated with a specific downregulation of E2F 1 independent of Rb and p 53 genomic status of cells . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Mdm 2 can also bind to other cellular proteins such as hNumb , E2F1 , Rb and Akt ; however , the biological significance of these interactions is less clear . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
To elucidate the biochemical processes involved in the pathogenesis of B CLL and MCL , we analyzed the expression level of a set of genes that play central roles in apoptotic or cell proliferation pathways and of candidate genes from frequently altered genomic regions , namely ATM , BAX , BCL 2 , CCND 1 , CCND 3 , CDK 2 , CDK 4 , CDKN1A , CDKN1B , E2F1 , ETV 5 , MYC , RB 1 , SELL , TFDP 2 , TNFSF 10 , and TP 53 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Potential mechanisms of growth inhibition , i . e . , regulation of the cell cycle control proteins E2F1 and retinoblastoma protein ( RB ) , and effect on activator protein 1 ( AP 1 ) mediated gene regulation were examined . beta apo 14 ' CA and atRA inhibited the expression of E2F1 protein in MCF 7 and Hs578T cells . beta apo 14 ' CA , beta apo 12 ' CA and atRA down regulated RB protein expression in MCF 7 but not in Hs578T cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In addition to overexpressed E2F1 , endogenous E2F1 released from Rb was also found to inhibit NF kappaB activity in a cell cycle dependent manner by using E2F1 ( + / + ) and E2F1 ( / ) murine embryonic fibroblasts . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Specificity of E2F1 , E2F2 , and E2F3 in mediating phenotypes induced by loss of Rb . ^^^ Here , we report that E2F1 , E2F2 , and E2F3 make major individual contributions toward the in vivo phenotypic consequences of Rb deficiency . ^^^ In the developing lens of Rb ( / ) embryos , loss of E2F1 , E2F2 , or E2F3 reduces the unscheduled proliferation of fiber cells , with the loss of E2F3 having the most pronounced effect . ^^^ In contrast , E2F1 is unique in mediating apoptosis in both Rb ( / ) lenses and retinas . ^^^ In the central nervous system , loss of E2F1 or E2F3 can almost completely eliminate the ectopic DNA replication and apoptosis observed in Rb ( / ) embryos , and loss of E2F2 partially reduces the unscheduled DNA replication and has no effect on apoptosis . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using a set of cell lines with differing p 53 and Rb status we determined that Mdm 2 induction of E2F1 transactivation was p 53 dependent , resulting from release of repression by p 53 . ^^^ Furthermore , E2F1 repression by an Rb phosphorylation mutant could not be reversed by Mdm 2 overexpression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Our results show that the expression of RB C pocket fragments not only induces delayed cell cycle progression , which is mediated by the down regulation of cyclin A , cyclin E , and E2F 1 , but also sensitizes cells to apoptosis through p 53 dependent pathways . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Activation of the Rb / E2F1 pathway by the nonproliferative p 38 MAPK during Fas ( APO1 / CD95 ) mediated neuronal apoptosis . ^^^ Aberrant activation of the Rb / E2F1 pathway in cycling cells , in response to mitogenic or nonmitogenic stress signals , leads to apoptosis through hyperphosphorylation of Rb . ^^^ To test whether in postmitotic neurons the Rb / E2F1 pathway can be activated by the nonmitogenic stress signaling , we examined the role of the p 38 stress activated protein kinase ( SAPK ) in regulating Rb phosphorylation in response to Fas ( CD95 / APO1 ) mediated apoptosis of cultured cerebellar granule neurons ( CGNs ) . ^^^ Hyperphosphorylation of Rb by p 38 SAPK resulted in the release of Rb bound E2F1 . ^^^ Taken together , these studies demonstrate that neuronal Rb / E2F1 is modulated by the nonproliferative p 38 SAPK in Fas mediated neuronal apoptosis . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Here , we show that deregulation of E2F by adenovirus E1A , loss of Rb or enforced E2F 1 expression results in the accumulation of caspase proenzymes through a direct transcriptional mechanism . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Our experiments show that a 53 amino acid stretch of E2F1 is sufficient for being targeted by prohibitin ; fusion of this region to GAL 4 VP16 construct could make it susceptible to prohibitin mediated , but not Rb mediated repression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Silibinin treatments also resulted in an increase in the amount of Rb binding to E2F1 ( 3 . 8 fold ) , E2F2 ( 2 . 2 fold ) , and E2F3 ( 2 . 2 fold ) . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The initiation of this TP 53 dependent G 1 phase arrest occurs despite the presence of substantial levels of cyclin D1 / CDK4 and cyclin E / CDK2 kinase activities , hyperphosphoryated RB , and active E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The E2F 1 transcription factor is a critical downstream target of the tumor suppressor , RB . ^^^ These data demonstrate that ATM is transcriptionally regulated by E2F 1 and suggest that ATM serves as a novel , ARF independent functional link between the RB / E2F pathway and p53 . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
IFN gamma inhibits human airway smooth muscle cell proliferation by modulating the E2F 1 / Rb pathway . ^^^ Induction of E2F 1 protein and Rb hyperphosphorylation after mitogen stimulation was also suppressed by IFN gamma . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The immunohistochemical studies were performed for retinoblastoma ( Rb 1 ) and E2F 1 , ( KH 95 ) after heat induced epitope retrieval . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
There was also a decrease in the expression of hyper phosphorylated retinoblastoma protein , the transcription factor E2F1 , and proliferating cell nuclear antigen , and there was an increase in expression of hypophosphorylated Rb and the cdk inhibitor p 16 ( Ink4a ) with increasing p 75 ( NTR ) expression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The human E2F 1 promoter has been shown to be selectively activated / derepressed in tumor cells with a defect in the Rb pathway . ^^^ We demonstrate that the antitumor activity of Ar6pAE2fF in vitro and in vivo is dependent on the E2F 1 promoter driving E1A expression in Rb pathway defective cells , and furthermore , that its oncolytic activity is enhanced by viral replication . ^^^ Selective oncolysis by Ar6pAE2fF was dependent on the presence of functional E2F binding sites in the E2F 1 promoter , thus linking antitumor viral activity to the Rb pathway . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Ebp 1 binds the tumor suppressor retinoblastoma protein ( Rb ) both in vivo and in vitro , and Rb and Ebp 1 cooperate to inhibit the transcription of the E2F1 regulated cyclin E promoter . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using such analysis , E2f 1 , E2f 3 , p 53 , and Id 2 have been identified as important regulators of cell cycle control and apoptosis in Rb ( / ) embryos . ^^^ As a transcriptional target of E2F 1 , a regulator of p 53 , and an important mediator of apoptosis , ARF was a strong candidate for such a role , especially since it can be upregulated in the absence of Rb . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
These changes were accompanied by increased expression of S and G ( 2 ) phase cyclins and cyclin dependent kinases ( cdk ) , increased Rb phosphorylation , and increased E2F 1 DNA binding activity . ^^^ In contrast , higher doses of RA ( 40 nM to 1 micro M ) inhibited ERK 1 expression , caused accumulation of G ( 1 ) phase cyclins and cdks , decreased Rb phosphorylation , and increased Rb / E2F 1 association . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The authors studied the effect of the chemokine stromal cell derived factor ( SDF ) 1alpha , the natural CXCR 4 ligand , and an X 4 using variant of gp 120 on the activity of cell cycle proteins involved in neuronal apoptosis and differentiation , such as Rb and E2F 1 . ^^^ Changes in expression , localization , and phosphorylation / activation of Rb and E2F 1 induced by SDF 1alpha ( 20 nM ) gp 120 ( IIIB ) ( 200 pM ) were analyzed in primary cultures of rat neurons and in a human cell line expressing recombinant CXCR 4 . ^^^ SDF 1alpha , which is able to rescue these neurons from apoptosis , induces a time dependent increase of total Rb expression while decreasing the nuclear content of phosphorylated ( Ser780 / Ser795 ) Rb and the transcriptional activity of E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
MDM 2 also interacts with the retinoblastoma protein ( RB ) and the transcription factor E2F1 to promote cell cycle S phase entry . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Furthermore , the decrease in promoter activity induced by expression of E2F 1 and Rb was reversed by trichostatin A treatment of Saos 2 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Involvement of the transcription factor E2F1 / Rb in kainic acid induced death of murine cerebellar granule cells . ^^^ However , hyperphosphorylation of Rb showed a transient increase which may lead to activation of E2F1 . ^^^ Therefore activation of Rb / E2F1 pathway appears to involve novel interactions yet to be elucidated . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Retinoblastoma ( Rb ) and E2F1 are critical cell cycle regulators that also have been implicated in several differentiation systems . ^^^ Here , we demonstrate that C / EBPepsilon interacts with Rb and E2F1 during granulocytic differentiation in NB 4 and U 937 human myeloid cells and in 32Dcl3 murine myeloid precursor cells . ^^^ We propose that the interactions between C / EBPepsilon , a tissue specific transcription factor , and the broad spectrum proteins , Rb and E2F1 , are important in C / EBPepsilon induced terminal granulocytic differentiation . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The expressions of N myc , cyclin D 3 , and Wnt10B were downregulated , whereas those of retinoblastoma ( RB ) and related genes ( p 107 , RB2 / p130 , p300 / CBP , E2F 1 , DP 1 ) as well as others were upregulated . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In DU 145 cells , p 21 levels were elevated and E2F1 levels decreased , although mutant p 53 , Rb , and Bax levels remained unchanged . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Over expression of E2F1 sustained an increased Chk 1 promoter activity , whereas overexpression of Rb protein , which binds E2F factors , decreased Chk 1 promoter activity . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Additionally , TNF inhibited Rb phosphorylation and dissociation from E2F1 . ^^^ Overexpression of constitutively active p 38 MAPK facilitated Rb E2F1 dissociation , whereas that of dominant negative p 38 MAPK did not . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The transcription factor E2F 1 is implicated in the activation of S phase genes as well as induction of apoptosis , and is regulated by interactions with Rb and by cell cycle dependent alterations in E2F 1 abundance . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry was used to examine the protein expression of CyclinD 1 , p 16 , E2F 1 and Rb in 4 groups ( 196 cases ) with mild papillomatosis ( MP ) , moderate papillomatosis ( MoP ) , serious papillomatosis ( SP ) and ductal carcinoma in situ ( DCIS ) . ^^^ RESULTS : There were significant difference of the positive rate and the intensity of CyclinD 1 , p 16 , E2F 1 and Rb expression among the groups , respectively ( chi ( 2 ) were 16 . 702 , 20 . 742 , 40 . 335 , 42 . 317 ; 19 . 120 , 29 . 469 , 45 . 080 , 46 . 920 , P < 0 . 01 . ^^^ There was significance of E2F 1 or Rb expression between SP and DCIS ( P < 0 . 01 ) , but there was no significance to be found in the expression of CyclinD 1 or p 16 between the two groups ( P > 0 . 05 ) . ^^^ CONCLUSION : CyclinD 1 , p 16 , E2F 1 and Rb played the important roles from MP into SP and then DCIS . ^^^ E2F 1 and Rb can be used as the assistant indicators on the differentiation of SP and DCIS . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
TRIP Br 1 was recently detected by two research groups , which described two separate functions , namely that of a transcriptional integrator of the E2F1 / DP1 / RB cell cycle regulatory pathway ( and then named TRIP Br 1 ) , and that of an antagonist of the cyclin dependent kinase suppression of p16INK4a ( and then named p34SEI 1 ) . ^^^ Our observations imply that the cell cycle promoting transcription factor E2F1 / DP1 is dually targeted by HPV oncoproteins , namely ( 1 ) by interference of the E 7 protein with repression by RB , and ( 2 ) by the transcriptional cofactor function of the E 6 protein . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Here we explored the hypothesis that new synthesis of E2F 1 is required for insulin like growth factor 1 ( IGF 1 ) to induce cyclin A accumulation and RB hyperphosphorylation , events that are targeted by tumor necrosis factor alpha ( TNFalpha ) to arrest cell cycle progression . ^^^ We first established that IGF 1 increases expression of cyclin A , causes hyperphosphorylation of RB , and augments the mass of E2F 1 in a time dependent manner . ^^^ Most important , this E2F 1 small interfering RNA also blocks the ability of IGF 1 to increase cyclin A accumulation and to hyperphosphorylate RB . ^^^ Collectively , these results establish that TNFalpha targets IGF 1 induced E2F 1 synthesis , leading to inhibition of the subsequent accumulation in cyclin A , formation of cyclin A Cdk 2 complexes , hyperphosphorylation of RB , and cell cycle arrest . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Deregulation of the Rb pathway by overexpression of E2F1 also induced caspase 9 dependent , Apaf 1 independent apoptosis in myoblasts . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , the activity of Rb appeared to be unchanged between treated and nontreated RD cells , as determined by the ability of Rb to bind E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We show that repression by Rb at E2F target gene promoters involves the establishment of a stable repressor complex that is not displaced by the overexpression of E2F 1 . ^^^ Rather than displacing Rb , excess E2F 1 instead recruits more Rb , leading to direct transcriptional repression . ^^^ In contrast , the Rb family members , p 130 and p 107 , which have not been demonstrated to be tumor suppressors , bind preferentially to target promoters in the absence of growth factors and in proliferating cells , respectively , and these repressor complexes are displaceable by E2F 1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Overexpression of E2F1 associated with LOH at RB locus and hyperphosphorylation of RB in non small cell lung carcinoma . ^^^ PURPOSE : E2F1 plays a critical role in cell proliferation , and its function is controlled by the retinoblastoma ( RB ) protein . ^^^ We examined the expression of E2F1 and the aberration of RB gene and protein to elucidate what factors contribute to the overexpression of E2F1 in non small cell lung carcinomas . ^^^ Immunohistochemical positivity for phosphorylated RB protein was also closely correlated with overexpression of E2F1 . ^^^ CONCLUSIONS : Our results suggest that overexpression of E2F1 , induced both by LOH at the RB locus and anomalous phosphorylation of the RB protein , is involved in the development of non small cell lung carcinoma . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Here we define a transcription mediated pathway in which deregulation of E2F1 by ectopic E2F expression or Rb inactivation by E 7 of human papillomavirus type 16 signals apoptosis by inducing the expression of Chk 2 , a component of the DNA damage response . ^^^ Our results suggest that E2F1 plays a central role in signaling disturbances in the Rb growth control pathway and , by upregulation of Chk 2 , may sensitize cells to undergo apoptosis . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also show that LRBA promoter activity and endogenous LRBA mRNA levels are reduced by p 53 and increased by E2F1 , indicating that mutations in the tumor suppressors p 53 and Rb could contribute to the deregulation of LRBA . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
E2F1 in particular , serves as the primary link between loss of Rb function and activation of p 53 dependent apoptosis . ^^^ E2F1 function is crucial for responding to loss of proper Rb mediated growth control to activate p 53 and the apoptotic program . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
To assess the cellular mechanisms underlying the mesangial cell proliferation and glomerulosclerosis in progressive human IgA nephropathy ( IgAN ) , we examined the expression of E2F1 , Rb , c Myc , proliferating cell nuclear antigen ( PCNA ) , cyclins ( D 1 , E and A ) , cyclin dependent kinase 2 ( CDK 2 ) and CDK inhibitors ( p 21 ( waf 1 ) , p 27 ( kip 1 ) , 57 ( kip 2 ) and p 16 ( ink4a ) ) by immunohistochemistry in renal biopsy specimens . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
An LXCXE motif on ASK 1 was required for Rb binding ; this correlated with increased E2F1 transcriptional activity and up regulation of the proapoptotic protein p 73 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We show that core protein suppresses the expression of tumor suppressor protein p 53 and cyclin dependent kinase ( CDK ) inhibitor p 21 and enhances the activation of cyclin dependent kinase 2 ( CDK 2 ) , the phosphorylation of retinoblastoma ( Rb ) , the activation of the transcription factor E2F 1 , and the expression of E2F 1 and S phase kinase interacting protein 2 ( SKP 2 ) genes . ^^^ Pretreatment of core protein expressing cells with the inhibitor of CDK 2 , Butyrolactone 1 , abolished the phosphorylation of Rb , the activation of E2F 1 , and inhibited the expression of E2F 1 gene and cell growth induced . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma gene product , Rb , is a major negative regulator of E2F1 activity ; however , Rb protein levels were found to be normal in MDS marrow samples . ^^^ Amplification of genomic DNA by the polymerase chain reaction ( PCR ) showed no E2F1 gene amplification or mutation in the Rb binding region of E2F1 in MDS patients , nor was transcriptional up regulation noted when E2F1 messenger RNA ( mRNA ) levels were estimated with real time reverse transcriptase PCR . ^^^ Previously , increased E2F1 activity in human disease had been found primarily as a consequence of Rb derailment . ^^^ Hence , the observation in MDS of increased E2F1 activity in the presence of normal Rb levels is novel and unique , and E2F1 activity in association with apoptosis in S phase cells may thus have significant therapeutic implications . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
CONCLUSIONS : The regulatory pathway of Rb / E2F 1 is associated with the cell proliferation and in differentiation of AB . ^^^ The activity or release of telomerase may be related to the lower expression of Rb and higher expression of E2F 1 , and is up regulated in G ( 1 ) late phase by cyclin E . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Our results demonstrated that 16 genes ; bad , bax , bcl 2 , bcl w , bcl 10 , caspase 3 , caspase 7 , caspase 8 , c myc , E 124 , GADD 45 , mdm 2 , NKkappab 1 , p 53 , p 21 , Rb and trail were up regulated and six genes ; caspase 1 , caspase 2 , DR 5 , E2F1 , FasL and iNOS did not changed in response to DES treatment in wild type mice compared to p53+ / knockout mice . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Downstream in CDKI CDK cyclin cascade , consistent with its inhibitory effect on CDK kinase activity , IP 6 treatment of cells increased hypophosphorylated levels of retinoblastoma ( Rb ) with a decrease in Rb phosphorylation at serine 780 , 807 , and 811 sites , and caused a moderate to strong decrease in the levels of transcription factors E2F1 , E2F4 , and E2F5 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
TNFalpha reduced the transcripts and protein levels of phosphorylated retinoblastoma protein ( Rb ) , Rb , E2F1 , and Cdk 4 only in drug resistant p 35 colo201 cells . ^^^ This effect of TNFalpha was reversed by IKK inhibitor , suggesting that TNFalpha induced cell cycle arrest is probably due to the reduction of Rb , E2F1 , and Cdk 4 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
DHT induced E2F 1 protein and mRNA expressions was also inhibited by BMP 2 , suggesting that BMP 2 inhibits DHT induced growth of LNCaP cells through a decrease in E2F protein expression and suppression of E2F activity by hypophosphorylation of Rb . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
However , inactivation of Rb or substitution of the E2F4 C terminal domain by the E2F1 C terminal region rescues the transformation defect . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also showed that during granulocytic differentiation of KCL 22 cells , the C / EBPdelta protein was detected in immunocomplexes with both Rb and E2F1 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Likewise , the combined deletion of Rb plus p 130 up regulated myocardial expression of Myc , E2F 1 , and G 1 cyclin dependent kinase activities , synergistically . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
This indicates that hyperphosphorylated Rb itself , dissociated from E2F1 transcription factor , can contribute directly or indirectly to tumor cell apoptosis provoked by HDAC inhibitors . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We also find that loss of Rb leads to deregulated E2F activity , with increased expression of E2F target genes , and that exogenous expression of E2F1 results in elevated COX 2 mRNA and protein levels . ^^^ Further analysis revealed that the E2F1 target gene , c myb , is elevated in Rb / PrE cells and E2F1 overexpressing cells , whereas ectopic overexpression of c myb activates the COX 2 promoter in prostate epithelial cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Flavopiridol ( FP ) , an inhibitor of cyclin dependent kinases , causes cell cycle arrest or apoptosis depending on the relation of the transcription factor E2F1 and RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Depletion of p28GANK inhibited HCC cell growth and tumorigenesis , enhanced dephosphorylation of RB 1 , and decreased transcription activity of E2F 1 in HuH 7 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Detailed analysis of expression of selected genes in beta carotene treated LNCaP cells at the level of mRNA and protein indicated that the observed increase of proliferation could have been the result of slight induction of a few genes affecting proliferation ( c myc , c jun ) and apoptosis ( bcl 2 ) with no significant effect on major cell cycle control genes ( cdk 2 , RB , E2F 1 ) . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Following the subcloning of the promoter regions into a gene reporter system , we found that at least four promoter haplotypes associated with CCND 1 , E2F1 , HDAC 1 and RB 1 significantly influenced transcriptional activity in an allele specific manner . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
This amazing synergism was accompanied by synergistic modulation of E2F 1 and p 27 ( Kip 1 ) , which were reported to mediate the anticancer activities of lovastatin and troglitazone , respectively , and other cell cycle regulating proteins such as CDK 2 , cyclin A and RB phosphorylation status . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
C / EBPbeta was unable to inhibit proliferation of MEFs lacking all three RB family proteins or wild type cells expressing dominant negative E2F 1 and , instead , stimulated their growth . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
BACKGROUND : The retinoblastoma ( Rb ) pathway , which governs cell cycle progression , is frequently genetically altered in cancer , causing deregulated expression of the E2F 1 transcription factor , which promotes DNA synthesis and cell cycle progression . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Western blot analyses showed that hyperphosphorylated Rb and E2F 1 were decreased , whereas hypophosphorylated Rb was increased . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We determined the mRNA expression of p 1 ( INK4A ) , p 14 ( ARF ) , CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 by quantitative reverse transcription PCR in 38 cases of GISTs and correlated the findings with clinicopathologic factors , including mutation analysis of KIT and PDGFRA . ^^^ GISTs with low mRNA expression of the CDKN2A transcripts p 16 ( INK4A ) and p 14 ( ARF ) but high mRNA expression of CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 were associated with aggressive clinical behavior and unfavorable prognosis , whereas GISTs with a low mRNA expression of CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 were not . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Rb inactivation leads to E2F1 mediated DNA double strand break accumulation . ^^^ We also find E2F1 associated DSBs in an Rb mutated cancer cell line in the absence of an exogenous DSB stimulus . ^^^ Thus , Rb status is key to regulating both the proliferation promoting functions associated with E2F and for preventing DNA damage accumulation if E2F1 becomes deregulated . ^^^ Taken together , these data suggest that loss of Rb creates strong selective pressure , via DSB accumulation , for inactivating p 53 mutations and that E2F1 contributes to the genetic instability associated with transformation and tumorigenesis . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Cell cycle regulators such as E2F1 and retinoblastoma ( RB ) play crucial roles in the control of adipogenesis , mostly by controlling the transition between preadipocyte proliferation and adipocyte differentiation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
We have mutated Rb 1 in the mouse to express a protein ( R654W ) specifically deficient in binding E2F1 , E2F2 , and E2F3 . ^^^ R654W mutant embryos exhibit cell cycle defects the same as those of Rb 1 null embryos , reinforcing the importance of the interactions of pRb with E2F1 , E2F2 , and E2F3 for cell cycle control . ^^^ These data indicate that Rb 1 makes important cell type specific contributions to cellular differentiation that are genetically separable from its general ability to stably bind E2F1 , E2F2 , and E2F3 and regulate the cell cycle . . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
BACKGROUND : To clarify the roles of cell cycle regulation in oncogenesis and cytodifferentiation of odontogenic tumors , expression of retinoblastoma protein ( RB ) and E 2 promoter binding factor 1 ( E2F 1 ) was analyzed in ameloblastomas as well as in tooth germs . ^^^ METHODS : Tissue specimens of 10 tooth germs , 40 benign ameloblastomas , and five malignant ameloblastomas were examined immunohistochemically with the use of antibodies against RB , E2F 1 , and phosphorylated RB . ^^^ RESULTS : Immunohistochemical reactivity for RB , E2F 1 , phosphorylated RB , and Ki 67 was detected in the nuclei of odontogenic epithelial cells near the basement membrane in tooth germs and benign and malignant ameloblastomas . ^^^ The number of cells positive for phosphorylated RB was nearly equal to or slightly less than the number of cells positive for RB or E2F 1 . ^^^ The number of Ki 67 positive cells was slightly more than the numbers of cell positive for RB , E2F 1 , or phosphorylated RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Attempted cell cycle induction in post mitotic neurons occurs in early and late apoptotic programs through Rb , E2F1 , and caspase 3 . ^^^ Either the absence or dysfunction of a number of critical pathways , such as those that involve the nuclear retinoblastoma protein ( Rb ) and the transcription factor E2F1 , may account for the aberrant induction of the cell cycle in post mitotic neurons that can be responsible for oxidative stress induced apoptotic cellular destruction . ^^^ Yet , it is unclear whether early programs of apoptotic injury that involve membrane phosphatidylserine ( PS ) exposure and calreticulin expression as well as later phases of apoptotic injury with nuclear DNA injury require the critical modulation of Rb and E2F1 . ^^^ We demonstrate that both the post translational of phosphorylation of Rb to prevent E2F1 transcription as well as the protein integrity of Rb are closely aligned with the modulation of cell cycle induction in post mitotic neurons during oxidative stress . ^^^ Progression of attempted cell cycle induction is closely associated with the phosphorylation of Rb , its inability to bind to E2F1 , and the degradation of the Rb protein . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Moreover , ASK 1 expression reverses the negative effect of Rb on E2F1 activity . ^^^ These results indicate that ASK 1 induction by E2F1 provides positive feedback regulation of E2F1 activity via Rb inhibition , which allows an efficient E2F1 Bim activation . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
To improve the selectivity towards RB pathway defective tumors and reduce the toxicity of Ad Delta24RGD we aimed to control E1A Delta 24 expression under the E2F 1 promoter . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Herein we show that E2F1 , a transcription factor essential to a cell cycle progress and a main target of tumor suppressor Rb , is a critical barrier for the induction of senescence . ^^^ Importantly , it appears that E2F1 depletion induced cancer cell senescence is not reliant on the integrity of either Rb or p 53 . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation assays demonstrated that nicotine stimulation caused an increased recruitment of E2F1 and concomitant dissociation of retinoblastoma tumor suppressor protein ( Rb ) from survivin promoter in A 549 cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
In reporter assays , PRMT 2 repressed E2F1 transcriptional activity in an RB dependent manner . ^^^ PRMT 2 formed a ternary complex with E2F1 in the presence of RB . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Immunohistochemical stains were carried out on tissue microarrays to evaluate the expression of proteins involved in the G ( 1 ) S transition and proteins that regulate apoptosis including Rb , E2F1 , cyclin D 1 , CDK 4 , CDK 6 , p 27 ( KIP 1 ) , p 21 ( WAF1 / CIP1 ) , p 53 , Mdm 2 , Bcl 2 , and Bax . ^^^ The positive phenotypes identified were as follows : Rb , 39 . 1 % ; E2F1 , 69 . 6 % ; cyclin D 1 , 30 . 4 % ; CDK 4 , 100 % ; CDK 6 , 30 . 4 % ; 39 . 1 % ; p 27 ( KIP 1 ) , 47 . 8 % ; p 21 ( WAF1 / CIP1 ) , 39 . 1 % ; p 53 , 43 . 5 % ; Mdm 2 , 17 . 4 % ; Bcl 2 , 91 . 3 % ; and Bax , 100 % . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Using neocortical extracts , we detected GA binding protein alpha ( GABPalpha , an Ets family member ) bound to the activator site and both E2F1 and E2F4 bound to the repressor site of the Rb promoter in vitro . ^^^ Additionally , we detected binding of both E2F1 and E2F4 to the Rb promoter in vivo using chromatin immunoprecipitation analysis on embryonic day 13 . 5 brain . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
Examination of the Rb pathway revealed that E2F 1 and p15INK4b expression fluctuated with increasing passages , with levels higher in passage 95 SC 2 cells compared to primary passage 6 CEF cells . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
A substantial quantity of active Rb binds more efficiently to E2F1 in cells that express Lf and consequently blocks the expression of an E2F1 responsive gene , thereby suggesting that Lf plays a crucial role in the inhibition of tumor cell growth . ^^^
Interacting proteins: Q01094 and P06400	Pubmed	SVM Score :0.0
The products were used to screen lambda gt 11 expression libraries , from which we cloned a cDNA encoding a polypeptide ( RBAP 1 ) capable of binding directly to a putative functional domain ( the pocket ) of the retinoblastoma gene product ( RB ) . ^^^ Therefore , RBAP 1 is a species of E2F and can bind specifically to the RB pocket . . ^^^