| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The basal promoter , a template containing a TATA box devoid of upstream regulatory sequences , has been used to identify and characterize the factors which , together with RNA polymerase 2 , govern transcription in mammalian systems : TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIG , TFIIH , and TFIIJ . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These factors , TFIIH and TFIIJ , were required together with the previously described general factors ( TFIIA , TFIIB , TFIID , TFIIE , and TFIIF ) and RNA polymerase 2 for transcription of different class 2 genes . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Consistent with these characteristics , HTLV 1 transcription activity was reconstituted in vitro by using TATA binding protein , TFIIA , recombinant TFIIB , TFIIE , and TFIIF , TFIIH , and pol 2 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The immunoprecipitate includes RNA polymerase 2 , TFIID , TFIIB , TFIIH , TFIIF , and TFIIE , but is devoid of transcriptional activator proteins , such as HNF 1 , HNF 4 , and C / EBP alpha . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| During this transition , TFIID remains promoter bound whereas TFIIB , TFIIE , TFIIF , and TFIIH are released . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| EBNA 2 has an acidic activation domain which interacts with general transcription factors TFIIB , TFIIH , and TAF 40 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TFIIE is a heterodimer composed of 56 kDa ( p 56 ) and 34 kDa ( p 34 ) subunits and functions in concert with the TATA factor , TFIIB , TFIIF , and TFIIH to promote formation of the RNA polymerase 2 preinitiation complex . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The mechanism by which site specific transcription factors increase specific initiation at polymerase 2 dependent promoters is thought to occur via recruitment and stabilization of components that form the initiation complex , i . e . , TFIID , TFIIA , TFIIB , TFIIE , TFIIG , TFIIH , TFIIJ and pol 2 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Using fluorescence in situ hybridization ( FISH ) , we show here that the genes encoding the TATA box binding protein ( TBP ) , TFIIB , TFIIE alpha , TFIIE beta , RAP 30 , RAP 74 and the 62 kDa subunit , of TFIIH are located at the human chromosomal bands 6q26 27 , 1p21 22 , 3q21 24 , 8p12 , 13q14 , 19p13 . 3 and 11p14 15 . 1 , respectively . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Three components , counterparts of human TFIIB , TFIIE , and RNA polymerase 2 , could not be exchanged individually but could be swapped in the pairs TFIIE TFIIH and TFIIB RNA polymerase 2 , which demonstrates that there are functional interactions between these components . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| First , we observe that synthesis of the first phosphodiester bond of transcripts initiated at the adenovirus 2 major late promoter depends strongly on ATP , TFIIE , and TFIIH in a transcription system reconstituted with RNA polymerase 2 , TFIIH , and recombinant TBP , TFIIB , TFIIE , and TFIIF . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In the same assay , ICP 4 was unable to interact with the basal transcription factors , TFIIB , TFIIE , TFIIF , and TFIIH and RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We show that pX interacts with general transcription factors TFIIB and TFIIH , as well as with the potent activation domain of VP 16 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Only 4 of 10 possible interactions were found above the detection limit : TFIIB , E , and F binding to RNA polymerase 2 and TFIIE binding to TFIIH . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Here we report that Gal11p stimulates basal transcription in a reconstituted transcription system composed of recombinant or highly purified transcription factors , TFIIB , TFIIE , TFIIF , TFIIH , and TATA box binding protein and core RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The Cdc73p Paf1p RNA polymerase 2 containing complex also includes Gal11p , and the general initiation factors TFIIB and TFIIF , but lacks TBP , TFIIH , and transcription elongation factor TFIIS as well as the Srbps . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The RNA polymerase 2 complex isolated using this procedure contains SRB ( suppressor of RNA polymerase B ) polypeptides , transcription factors IIE and IIF , limiting amounts of TFIIH , and the TATA binding protein , but was devoid of TFIIB . . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| With the availability of the general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , it is now possible to investigate aspects of the mechanism of eukaryotic messenger RNA synthesis in purified , reconstituted RNA polymerase 2 transcription systems . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| This holoenzyme contained nearstoichiometric amounts of all the general transcription factors , TFIIB , TFIID ( TBP + TAFIIs ) , TFIIE , TFIIF , and TFIIH , required to accurately initiate transcription in vitro at the adenovirus major late promoter . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Hepatitis B virus pX targets TFIIB in transcription coactivation . pX , the hepatitis B virus ( HBV ) encoded regulator , coactivates transcription through an unknown mechanism . pX interacts with several components of the transcription machinery , including certain activators , TFIIB , TFIIH , and the RNA polymerase 2 ( POLII ) enzyme . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The core promoter is the site for assembly of the transcription preinitiation complex , which includes RNA pol 2 and the general transcription fctors TBP , TFIIB , TFIIE , TFIIF , and TFIIH . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| By exploiting adenovirus 2 major late promoter derivatives that contain premelted transcriptional start sites and do not require TFIIF , TFIIE , or TFIIH for transcription initiation , we observe ( 1 ) that Elongin is capable of strongly stimulating the rate of synthesis of trinucleotide transcripts by a subcomplex of RNA polymerase 2 , TBP , and TFIIB and ( 2 ) that the ability of Elongin to stimulate synthesis of these short transcripts is substantially reduced by addition of TFIIF to transcription reactions . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The DNA wrapping model describes specific roles for general RNA polymerase 2 transcription factors ( TATA binding protein [ TBP ] , TFIIB , TFIIF , TFIIE , and TFIIH ) , provides a plausible explanation for preinitiation complex isomerization , suggests mechanisms underlying the synergy between transcriptional activators , and suggests an unforseen role for TBP associating factors in transcription . . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Our results show that a system consisting of purified recombinant TFIIA , TFIIB , TFIIE , TFIIF , CREB , and Tax , along with highly purified RNA polymerase 2 , affinity purified epitope tagged TFIID , and semipurified TFIIH , supports basal transcription of the HTLV 1 promoter but is not responsive to Tax . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These studies led to the discovery of the class of general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , which function in intimate association with RNA polymerase 2 and are required for selective binding of polymerase to its promoters , formation of the open complex , and synthesis of the first few phosphodiester bonds of nascent transcripts . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Interestingly , multiple basal transcription factors for RNA polymerase 2 , including TBP , TFIIB , TFIIH , and TFIIF , were found to be associated with MSS 1 . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Using order of addition and template challenge assays performed in a human cell free transcription system reconstituted with recombinant general transcription factors ( TFIIB , TBP , TFIIE , TFIIF ) , a recombinant general cofactor ( PC 4 ) , and highly purified epitope tagged multiprotein complexes ( TFIID , TFIIH , pol 2 ) , we demonstrate that when TBP is used as the TATA binding factor transcriptional activators such as Gal 4 VP16 and human papillomavirus E 2 mainly function by facilitating pol 2 entry to the promoter region . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| A mouse in vitro transcription system reconstituted from highly purified RNA polymerase 2 , TFIIH and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| This model stipulates that wrapping of the promoter DNA around RNAPII and the general initiation factors TBP , TFIIB , TFIIE , TFIIF and TFIIH induces a torsional strain in the DNA double helix that facilitates strand separation and open complex formation . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The measurements showed comparable amounts of RNA polymerase 2 , TFIIE , and TFIIF , lower levels of TBP and TFIIB , and still lower levels of Mediator and TFIIH . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We have now purified S . pombe RNA polymerase 2 and its general initiation factors TFIIB , TFIIF , TFIIE , and TFIIH to near homogeneity . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The trajectory of promoter DNA determined by the C terminal domain of TFIIB traverses sites of interaction with TFIIE , TFIIF , and TFIIH , serving to define their roles in the transcription initiation process . . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| RNA polymerase 2 ( RNAPII ) requires a set of general transcription factors TFIIA , TFIIB , TFIID , TFIIE , TFIIF and TFIIH to initiate transcription from a gene promoter in vitro . ^^^ In this report , we describe a reconstituted in vitro transcription system that consists of the following preparations of factors from the yeast Schizosaccharomyces pombe : affinity purified RNAPII , TFIIH , and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We have studied repair and transcription activities at 8 oxoG lesions with a reconstituted transcription system ( RTS ; RNA polymerase 2 , TBP , TFIIA , TFIIB , TFIIE , TFIIF and TFIIH ) , as well as in cellular extracts and in vivo . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We have analyzed an interaction of the general transcription complex RNA polymerase 2 proteins ( RNA polymerase 2 , factors TBP , TFIIB , TFIIF , TFIIE and TFIIH ) S . cerevisiae with the oligoribonucleotides . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These studies indicate that the hsp 70 promoter is already occupied by TATA binding protein ( TBP ) and several TBP associated factors ( TAFs ) , TFIIB , TFIIF ( RAP 30 ) , TFIIH ( XPB ) , TBP free / TAF containg complex ( GCN 5 and TRRAP ) , and the Mediator complex subunit 13 before heat shock . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Also mapped were segments of TFIIB , TFIIE , TFIIF and TFIIH in proximity to promoter DNA . ^^^ |
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| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In eukaryotes , the core promoter serves as a platform for the assembly of transcription preinitiation complex ( PIC ) that includes TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIH , and RNA polymerase 2 ( pol 2 ) , which function collectively to specify the transcription start site . ^^^ |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13889 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|