| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| A complex formed with human TFIID and TFIIA ( DA complex ) was specifically recognized by TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These complexes were generated by sequential binding of TFIID , TFIIA , TFIIB , RNA polymerase 2 , and TFIIE . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| It binds effectively TATA binding protein with TFIIB and TFIIA in a synergistic manner . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Subsequent association of TFIIA and TFIIB provides enhanced complex stability . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Protein protein interactions between human heat shock transcription factor 1 ( hHSF 1 ) and general transcription factors TFIIA gamma , TFIIB , TBP , TAF ( 2 ) 32 , and TAF ( 2 ) 55 and positive coactivator PC 4 were characterized in order to identify potential targets of contact in the transcriptional preinitiation complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The interaction of Dr 1 with TBP precludes the formation of a transcription competent complex by inhibiting the association of TFIIA and / or TFIIB with TBP . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The basal promoter , a template containing a TATA box devoid of upstream regulatory sequences , has been used to identify and characterize the factors which , together with RNA polymerase 2 , govern transcription in mammalian systems : TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIG , TFIIH , and TFIIJ . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The heDAP fraction also behaved like TFIIA in its ability to form a promoter TFIID TFIIA complex and to recruit TFIIB to such a complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These factors , TFIIH and TFIIJ , were required together with the previously described general factors ( TFIIA , TFIIB , TFIID , TFIIE , and TFIIF ) and RNA polymerase 2 for transcription of different class 2 genes . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| RAP30 / 74 or recombinant RAP 30 suppresses nonspecific binding of RNA polymerase 2 to DNA and is required for RNA polymerase 2 to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID , TFIIA and TFIIB ; both RAP 30 and RAP 74 are physical components of the preinitiation complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| When reactions were reconstituted with the previously characterized class 2 transcription factors ( TFIIA , TFIIB , TFIID , TFIIE / F ) , transcription by human RNA polymerase 2 from the adenovirus major late promoter was essentially restricted to a single round of initiations . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Recombinant TFIID forms a stable complex on a TATA box either alone or in combination with either of the general transcription factors , TFIIA or TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TFIIB and TFIIE were also required , in addition to TFIIA , TFIID , RNA polymerase 2 , and the adenovirus 2 major late promoter , for the formation of a ( preinitiation ) complex that could initiate transcription ( upon addition of nucleoside triphosphates ) in the presence of heparin concentrations which inhibited the action of unbound factors . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Related Sarkosyl studies indicated that the inferred complex was subsequently recognized by RNA polymerase 2 , which resulted in an increased level of Sarkosyl resistant transcription ( in the presence of TFIIA and TFIID ) , and that this interaction occurred independently of TFIIB and TFIIE . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Consistent with these characteristics , HTLV 1 transcription activity was reconstituted in vitro by using TATA binding protein , TFIIA , recombinant TFIIB , TFIIE , and TFIIF , TFIIH , and pol 2 . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In vitro , N 2 1 fails to interact with TFIIA , but it associates normally with a TATA element , an acidic activation domain , and TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In a reconstituted in vitro transcription assay , Tax 1 transactivation was dependent upon basal transcription factors TFIIB , TFIIF , Pol 2 , TFIID , and TFIIA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TATA binding protein residues implicated in a functional interplay between negative cofactor NC 2 ( Dr 1 ) and general factors TFIIA and TFIIB . ^^^ Several negative cofactors ( NC 1 , NC 2 , and Dr 1 ) are known to interact with TBP in a manner that prevents productive interactions of transcription factors TFIIA and TFIIB with promoter bound TBP . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We further show that Msx 1 represses transcription in vitro in a purified reconstituted assay system and interacts with protein complexes composed of TBP and TFIIA ( DA ) and TBP , TFIIA , and TFIIB ( DAB ) in gel retardation assays , suggesting that the mechanism of repression is mediated through interaction ( s ) with a component ( s ) of the core transcription complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Furthermore , recombinant TFIIB appeared to influence the TFIID Inr interaction , whereas TFIIA stabilized the TFIID TATA interaction . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The promoter complex formed with this hybrid TFIIA depends upon the TATA element and can be efficiently incorporated into a higher order preinitiation complex upon addition of TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The formation of a stable preinitiation complex intermediate resistant to Sarkosyl required the preincubation of the promoter DNA with Zta , holo TFIID ( TBP and TBP associated factors ) , TFIIB , TFIIA , and the coactivator USA . ^^^ The formation of a Zta response element resistant preinitiation complex required the preincubation of promoter DNA with Zta , holo TFIID , TFIIB , and TFIIA . ^^^ These results provide functional and physical evidence that the Zta transcriptional activator influences at least two distinct steps in preinitiation complex assembly , the formation of the stable holo TFIID TFIIA promoter complex and the subsequent binding of TFIIB and a USA like coactivator . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| By using the adenovirus 2 major late promoter tagged site specifically with the photoactivatible cross linking reagent N3R dUMP we have localized TBP , two subunits of TFIIA ( A 35 and A 21 ) , TFIIB , and RAP 30 along promoter DNA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The mechanism by which site specific transcription factors increase specific initiation at polymerase 2 dependent promoters is thought to occur via recruitment and stabilization of components that form the initiation complex , i . e . , TFIID , TFIIA , TFIIB , TFIIE , TFIIG , TFIIH , TFIIJ and pol 2 . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| DNase 1 footprint titration was used to measure the effects of TFIIB and TFIIA on binding of TBP to a consensus TATA box . ^^^ Under reaction conditions optimized for TBP DNA complex formation , the presence of TFIIB increased affinity of TBP for the TATA box by 2 . 5 fold , while TFIIA had no effect . ^^^ When TBP binding conditions were sub optimal , both TFIIB and TFIIA independently increased TBP affinity by approximately 10 fold . ^^^ Therefore both TFIIB and TFIIA have the intrinsic ability to directly increase the affinity of TBP for the TATA box . ^^^ We suggest that this property of TFIIA and TFIIB may increase the range of conditions under which high affinity TBP DNA interactions can occur and may therefore favor the formation of the preinitiation complex . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The involvement of TFIID , TFIIA , a factor homologous to TFIIB and an RNA factor in class 3 gene transcription has further changed our thinking in regards to the mechanisms of transcription . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Purified recombinant CeTBP binds specifically to the TATA box sequence , interacts with transcription factors TFIIA and TFIIB , and is able to substitute for the TFIID basal activity when assayed by in vitro transcription in both HeLa and C . elegans nuclear extracts . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Transcription factor IID , possibly with the cooperation of TFIIA , binds to the TATA element of the promoter , forming a complex that can bind TFIIB ( refs 6 , 7 ) followed by RNA polymerase 2 ( refs 6 , 8 ) and other factors . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Association of DRAP 1 with Dr 1 results in higher stability of the Dr 1 TBP TATA motif complex and precluded the entry of TFIIA and / or TFIIB to preinitiation complexes . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TFIIA binds as a heterodimer to the side of the TBP / TATA complex opposite to the side that binds TFIIB and does not alter the TBP / DNA interaction . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| To localize the regions of the TBP DNA complex that interact with BRF 1 and TFIIIB 90 , we tested whether the pol 2 factors TFIIA and TFIIB interfered with the binding of BRF 1 and TFIIIB 90 to TBP DNA . ^^^ Our results suggest that the binding sites for BRF 1 and TFIIIB 90 on TBP DNA both overlap the binding sites for TFIIA and TFIIB . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Three of these derivatives interact normally with a TATA element , TFIIA , TFIIB , or an acidic activation domain ; presumably , they affect another protein protein interaction important for transcriptional activation . ^^^ The remaining derivative ( with F 237 replaced by D ) binds a TATA element with wild type affinity , but the TBP TATA complex has an altered electrophoretic mobility and interacts poorly with TFIIA and TFIIB ; this suggests that the conformation of the TBP TATA element complex plays a role in transcriptional activation . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TBP binds the transcriptionally inactive TA 5 sequence but the resulting complex is not efficiently recognised by TFIIB and TFIIA . ^^^ DNase 1 footprinting patterns of the TBP TAAAAA complex are different from those observed in functional TBP TATA box complexes , indicating that , most likely , it is a different spatial arrangement of the TBP DNA complex that prevents formation of the TFIIB TBP TAAAAA complex , also seriously impairing entry of TFIIA to the complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Regions on the surface of human TATA box binding protein ( TBP ) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo , basal transcription in vitro , and TFIIA and TFIIB binding in vitro . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| High resolution mapping of nucleoprotein complexes by site specific protein DNA photocrosslinking : organization of the human TBP TFIIA TFIIB DNA quaternary complex . ^^^ We have used a novel site specific protein DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary , ternary , and quaternary complexes containing human TATA binding protein , human or yeast transcription factor IIA ( TFIIA ) , human transcription factor IIB ( TFIIB ) , and promoter DNA . ^^^ The results indicate that TFIIA and TFIIB make more extensive interactions with promoter DNA than previously anticipated . ^^^ TATA binding protein , TFIIA , and TFIIB surround promoter DNA for two turns of DNA helix and thus may form a `` cylindrical clamp ' ' effectively topologically linked to promoter DNA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Gel mobility shift assays showed that NHP6A promotes the formation of a complex with TBP and TFIIA at the TATA box that has enhanced affinity for TFIIB . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Finally , when using TBP mutants defective on multiple interaction surfaces , we observed synthetic lethal effects between mutations on the TFIIA and TFIIB interfaces but found that mutations implicated in association with polymerase 2 and TFIIF did not have significant effects in vivo . ^^^ Taken together , these results argue that , unlike the TBP TATA and TBP TFIIA interactions , the TBP TFIIB interaction is not generally limiting for transcriptional activation in vivo . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TBP also associates with a number of basal transcription factors such as TFIIA and TFIIB , and with several regulatory factors such as VP 16 , E1A , and p 53 . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The general factors TFIIA and TFIIB each increase the rotational and translational selectivity of yTBP but are not sufficient , at least individually , to confer a unique polarity to the preinitiation complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Competition for binding to TBP by BRF and either TFIIB or TFIIA suggests that BRF binds on the opposite face of the TBP DNA complex from TFIIB and that the binding sites for TFIIA and BRF overlap . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Reconstitution experiments showed that the formation of a stable enhanceosome dependent preinitiation complex require cooperative interactions between the enhanceosome ; the general transcription factors TFID , TFIIA , and TFIIB ; and the cofactor USA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Bcl 3 specifically interacted with the general transcription factors TFIIB , TBP , and TFIIA but not with TFIIEalpha in the GST pull down assays . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Crude theaflavin was extracted from black tea and then fractionated by HPLC into five components ( initial peaks ( IP ) , TF 1 , TF2A , TF2B , and TF 3 ) . ^^^ The combination of TF 1 + TF2A + TF2B + TF 3 was more active than the sum of the activities of these four fractions individually , indicating synergism among the peaks . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Mutations in the CTD of Pol 2 , Srb 2 , Srb 4 , and Srb 5 , and two mutations in TFIIB disrupt recruitment of all holoenzyme components tested without affecting TFIID and TFIIA recruitment . ^^^ These and other results suggest that TFIIA acts at an early step by enhancing the stable recruitment of TFIID . ( 4 ) An extract containing the TFIIB mutant E62G , had no defect in PIC formation , but had a severe defect in transcription . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Like TRF 1 and TBP , TRF 2 binds transcription factor IIA ( TFIIA ) and TFIIB and appears to be part of a larger protein complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| General transcription factors TFIIA and TFIIB bound glutathione S transferase TRP in solution but failed to stimulate TRP binding to DNA . ^^^ TRP transcriptional repression activity was specifically reduced by mutations in TRP that disrupt the TFIIA binding surface but not by mutations that disrupt the TFIIB or DNA binding surface of TRP . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The multisubunit transcription factor IID ( TFIID ) is an essential component of the eukaryotic RNA polymerase 2 machinery that works in concert with TFIIA ( IIA ) and TFIIB ( IIB ) to assemble initiation complexes at core eukaryotic promoters . ^^^ TFIID is a trilobed , horseshoe shaped structure , with TFIIA and TFIIB bound on opposite lobes and flanking a central cavity . ^^^ Antibody studies locate the TATA binding protein ( TBP ) between TFIIA and TFIIB at the top of the cavity that most likely encompasses the TATA DNA binding region of the supramolecular complex . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Our results show that a system consisting of purified recombinant TFIIA , TFIIB , TFIIE , TFIIF , CREB , and Tax , along with highly purified RNA polymerase 2 , affinity purified epitope tagged TFIID , and semipurified TFIIH , supports basal transcription of the HTLV 1 promoter but is not responsive to Tax . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Negative cofactor 2 ( NC 2 ) is a dimeric histone fold complex that represses RNA polymerase 2 transcription through binding to TATA box binding protein ( TBP ) and inhibition of the general transcription factors TFIIA and TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NC 2 is a dimeric histone fold complex that represses RNA polymerase 2 transcription through binding to TBP and inhibition of TFIIA and TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Transcriptional activity in yeast strongly correlates with promoter occupancy by general factors such as TATA binding protein ( TBP ) , TFIIA , and TFIIB , but not with occupancy by TBP associated factors ( TAFs ) . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Virtually unidirectional binding of TBP to the AdMLP TATA box within the quaternary complex with TFIIA and TFIIB . ^^^ RESULTS : We used an affinity cleavage assay to explore the combined effects of TFIIA and TFIIB on the axial and orientational specificity of yTBP . ^^^ Our results show that the combination of TFIIA and TFIIB affixes yTBP in virtually a single orientation as well as a unique location on the AdMLP TATA box . ^^^ Ninety five percent of the quaternary TBP TFIIA TFIIB TATA complex contained yTBP bound in the orientation expected on the basis of crystallographic and genetic experiments , and more than 70 % is restricted axially to the 8 bp sequence TATAAAAG . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| A 96 . 7 kDa quaternary transcription factor complex consisting of the conserved core domains of yeast TBP , TFIIA , TFIIB and TATA box DNA has been assembled from purified components . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Most interestingly , other TBP mutants that interfere with different interactions ( TFIIB , TFIIA , or the TATA box ) and a TFIIB mutant defective for interaction with TBP all manifest distinct and selective promoter specific activation defects . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Here , we show that the p 50 subunit as well as the p50 / p65 of NF kappaB , and not other factors such as SP 1 , TFIIB , polymerase 2 , TFIIA , or p 65 , can be acetylated by CBP / p300 HAT domain . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| This increase in orientational specificity corresponds to a free energy difference ( Delta Delta G ( obs ) ) of 1 . 1 kcal 10 mol ( 1 ) and was accompanied by a dramatic increase in axial specificity , reminiscent of the effects of transcription factors TFIIB and TFIIA reported previously . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Green tea catechins , including ( ) epicatechin ( EC ) , ( ) epicatechin gallate ( ECG ) , ( ) epigallocatechin ( EGC ) and ( ) epigallocatechin gallate ( EGCG ) , are oxidized and dimerized during the manufacture of black tea and oolong tea to form orange red pigments , theaflavins ( TF ) , a mixture of theaflavin ( TF 1 ) , theaflavin 3 gallate ( TF2A ) , theaflavin 3 ' gallate ( TF2B ) and theaflavin 3 , 3 ' digallate ( TF 3 ) . ^^^ Analysis of the thiobarbituric acid reactive substances ( TBARS ) and conjugated dienes produced during LDL oxidation revealed that the antioxidant activity was in the order : TF 3 > ECG > EGCG > or = TF2B > or = TF2A > TF 1 > or = EC > EGC . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The typical TBP repeat signature and the amino acid motives involved in TFIIA and TFIIB interactions were conserved in this new TBP like protein . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Its inhibitory mechanism has been extensively characterized ; NC 2 binds to the TATA binding protein ( TBP ) , blocking the recruitment of TFIIA and TFIIB , and thereby inhibiting preinitiation complex assembly . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Interactions of a DNA bound transcriptional activator with the TBP TFIIA TFIIB promoter quaternary complex . ^^^ Site specific protein DNA photo cross linking was used to show that , when bound to its cognate site at various distances upstream of the TATA element , the chimeric transcriptional activator GAL 4 VP16 can physically interact with a TATA box binding protein ( TBP ) transcription factor IIA ( TFIIA ) TFIIB complex assembled on the TATA element . ^^^ This result implies DNA bending and looping of promoter DNA as a result of the physical interaction between GAL 4 VP16 and an interface of the TBP TFIIA TFIIB complex . ^^^ This protein protein interaction on promoter DNA minimally requires the presence of one GAL 4 binding site and the formation of a quaternary complex containing TBP , TFIIB , and TFIIA on the TATA element . ^^^ Notably , the topology of the TBP TFIIA TFIIB promoter complex is not altered significantly by the interaction with DNA bound activators . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Our simulated TATA / TBP complexes develop sequence dependent structure and motion trends that may lead to favorable orientations for high activity variants ( with respect to binding TFIIA , TFIIB , and other transcription factors ) , while conversely , accelerate dissociation of low activity TATA / TBP complexes . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| A novel TBP interacting zinc finger protein represses transcription by inhibiting the recruitment of TFIIA and TFIIB . ^^^ We also found through chromatin immunoprecipitation experiments that the interaction between NZFP and TBP inhibits binding of TFIIA and TFIIB . ^^^ These data strongly suggest that the repression by NZFP occurs through its binding to both DNA and TBP and the resulting NZFP TBP promoter complex inhibits preinitiation complex assembly by preventing binding of TFIIA and TFIIB . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In addition , EMSA studies show that adenovirus E1A ( 13S ) oncoprotein is unable to disrupt either the preestablished TFIIA / TBP / TATA or TFIIA / TFIIB / TBP / TATA complexes , but does inhibit complex formation when all transcription factors were simultaneously added . ^^^ The inhibitory effect of E1A ( 13S ) on the assembly of the PIC is overcome when excess TBP is added back in the reaction , while addition of either excess TFIIA or TFIIB were ineffective . ^^^ This work , coupled with previous findings in our laboratory , indicates that TFIIA is much more effective than TFIIB in reversing the inhibitory effect of HMGB 1 binding in the early stages of PIC assembly , which is consistent with the in vitro transcription results . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TBP 2 binds the TATA box , interacts with TFIIA and TFIIB ( similarly to TBP ) , and can mediate Pol 2 transcription initiation . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The potent transcriptional activation domain ( AD ) of the VP 16 protein of herpes simplex virus has previously been shown to interact with several general transcription factors including the TATA binding protein ( TBP ) , TBP associated factor 9 ( TAF 9 ) , TFIIA , and TFIIB . ^^^ In surface plasmon resonance assays , a module of the VP 16 AD designated VP16C ( residues 452 490 ) bound to TBP with an affinity notably stronger than to TAF 9 , TFIIA or TFIIB . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TATA interaction in preformed , higher order complexes containing the additional general transcription factors TFIIB and TFIIA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| As determined by sequential chromatin immunoprecipitation , Mot 1 co occupies promoters with TBP , but not with TFIIB , TFIIA , or Pol 2 when cells are grown in normal conditions . ^^^ Surprisingly , in response to heat shock and other forms of environmental stress , Mot 1 co occupies promoters with TFIIB and elongation competent Pol 2 , but not with TFIIA . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| RNA polymerase 2 ( RNAPII ) requires a set of general transcription factors TFIIA , TFIIB , TFIID , TFIIE , TFIIF and TFIIH to initiate transcription from a gene promoter in vitro . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The increased TFIIB binding correlates with VPN 2 ' s increased ability to recruit a complex containing TFIID , TFIIA and TFIIB . ^^^ The VPN subdomain also facilitates assembly of a complex containing TBP : TFIIA : TFIIB , which lacks TAFs , and provides a mechanism that could function at TAF independent promoters . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The TATA binding protein ( TBP ) , TFIIA , and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation , and many transcriptional regulators function by either stimulating or inhibiting formation of this complex . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| We have studied repair and transcription activities at 8 oxoG lesions with a reconstituted transcription system ( RTS ; RNA polymerase 2 , TBP , TFIIA , TFIIB , TFIIE , TFIIF and TFIIH ) , as well as in cellular extracts and in vivo . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Four antioxidant compounds , theaflavin ( TF 1 ) , theaflavin 3 gallate ( TF2A ) , theaflavin 3 ' gallate ( TF2B ) and theaflavin digallate ( TF 3 ) , were isolated from acetic acetate extract of black tea by silica gel and sephadex LH 20 column chromatography . ^^^ TF 1 , TF2A , TF2B and TF 3 , have stronger antioxidant activity than that of BHT ( Butylated hydorxytoluene ) . . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| A crucial step in eukaryotic transcriptional initiation is recognition of the promoter TATA by the TATA binding protein ( TBP ) , which then allows TFIIA and TFIIB to be recruited . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Each element was classified by its functional activity for in vitro interaction with TATA binding protein ( TBP ) , TFIIA , and TFIIB . ^^^ Certain off consensus TATA elements form poor binding sites for TBP and this compromised interaction interferes with higher order complex formation with TFIIA and / or TFIIB . ^^^ Another distinct category is comprised of two elements ( CATAAA and TATAAG ) , which are not affected in the initial formation of the TBP , TFIIA TBP , or TFIIB TBP complexes . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| In eukaryotes , the core promoter serves as a platform for the assembly of transcription preinitiation complex ( PIC ) that includes TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIH , and RNA polymerase 2 ( pol 2 ) , which function collectively to specify the transcription start site . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Remarkably , a large number of promoters assemble into partial preinitiation complexes ( partial PICs ) , containing TFIIA , TFIID ( and / or SAGA ) , TFIIB , TFIIE , and TFIIF . ^^^ |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52657 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|