Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Similar assays showed that TAFII 80 interacted with TFIIE alpha and with TFIIF alpha ( RAP 74 ) but not with TFIIB , TFIIE beta , or TFIIF beta ( RAP 30 ) . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Analysis of loci GTF2F1 and GTF2B , encoding Rap 74 ( a subunit of TFIIF ) and TFIIB , respectively , showed that they are present in a single copy in the human genome and are localized at 19p13 . 3 and 1p22 , respectively . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
RAP30 / 74 or recombinant RAP 30 suppresses nonspecific binding of RNA polymerase 2 to DNA and is required for RNA polymerase 2 to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID , TFIIA and TFIIB ; both RAP 30 and RAP 74 are physical components of the preinitiation complex . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Mammalian TFIIF , a heterodimer of approximately 30 kDa ( RAP 30 ) and approximately 70 kDa ( RAP 74 ) subunits , assists TFIIB in recruiting RNA polymerase 2 into the preinitiation complex and activates the overall rate of RNA chain elongation by suppressing transient pausing by polymerase at many sites on DNA templates . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Using fluorescence in situ hybridization ( FISH ) , we show here that the genes encoding the TATA box binding protein ( TBP ) , TFIIB , TFIIE alpha , TFIIE beta , RAP 30 , RAP 74 and the 62 kDa subunit , of TFIIH are located at the human chromosomal bands 6q26 27 , 1p21 22 , 3q21 24 , 8p12 , 13q14 , 19p13 . 3 and 11p14 15 . 1 , respectively . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Mutants were tested for accurate transcriptional activity , RAP 74 binding , and TFIIB binding . ^^^ The C terminal region of RAP 74 ( amino acids 358 517 ) binds directly and independently to TFIIB . ^^^ Interestingly , RAP 74 blocks TFIIB RAP 30 binding , both by binding TFIIB and by binding RAP 30 . ^^^ When the TFIIF complex is intact , therefore , TFIIB TFIIF contact is maintained through RAP 74 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Inr activity was not detected in reactions containing TFIIB , RAP 30 , RAP 74 , RNA polymerase 2 , and either TBP or TFIID , even though these factors were sufficient for TATA mediated transcription from supercoiled templates . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Msx 2 binds both components of TFIIF ( RAP 74 , RAP 30 ) , but not TFIIB or TBP . ^^^ Co expression of RAP 74 in MC3T3 E 1 cells partially reverses ( > 50 % ) suppression of OC promoter activity by Msx 2 , while co expression of TFIIB or RAP 30 has no effect . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The N terminal domain of RAP 74 ( minimally aa 1 to 172 ) is sufficient to deliver pol 2 into a complex formed on the adenovirus major late promoter with the TATA binding protein , TFIIB , and RAP 30 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Two portions of this carboxy terminal region of Fcp1p bound directly to the first cyclin like repeat in the core domain of the general transcription factor TFIIB , as well as to the RAP 74 subunit of TFIIF . ^^^ These regulatory interactions with Fcp1p involved closely related amino acid sequence motifs in TFIIB and RAP 74 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
According to solution and mutagenesis studies , the multiple domains of RAP 30 and RAP 74 bind PolII , TFIIB , TAF 250 and DNA in interactions that are essential for transcription initiation and elongation . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Solution structure of the carboxyl terminal domain of RAP 74 and NMR characterization of the FCP 1 binding sites of RAP 74 and human TFIIB . ^^^ The phosphatase activity of FCP 1 is strongly enhanced by the carboxyl terminal domain of RAP 74 ( cterRAP 74 , residues 436 517 ) , and this stimulatory effect of TFIIF can be blocked by TFIIB . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Interestingly both RAP 30 and RAP 74 interact with the same domain ( D 5 ) of the C terminal RMP of 118 amino acid residuals which overlaps with its TFIIB binding domain . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
FCP 1 , a phosphatase specific for the carboxyl terminal domain of the largest subunit of RNA polymerase 2 , is regulated by the HIV 1 Tat protein , CK 2 , TFIIB , and the large subunit of TFIIF ( RAP 74 ) . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
FCP 1 activity has been shown to be regulated by the general transcription factors TFIIF ( RAP 74 ) and TFIIB , protein kinase CK 2 ( CK 2 ) , and the HIV 1 transcriptional activator Tat . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The purified kinase phosphorylates native RNA polymerase 2 only in the presence of DNA and the general transcription factors TFIID ( TBP ) , TFIIB , and TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Basal transcription from the IgH promoter was reconstituted by TFIID , TFIIB , TFIIF , and polymerase , whereas basal transcription from the MLP is highly dependent upon TFIIE in addition to the above factors . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The basal promoter , a template containing a TATA box devoid of upstream regulatory sequences , has been used to identify and characterize the factors which , together with RNA polymerase 2 , govern transcription in mammalian systems : TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIG , TFIIH , and TFIIJ . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
These factors , TFIIH and TFIIJ , were required together with the previously described general factors ( TFIIA , TFIIB , TFIID , TFIIE , and TFIIF ) and RNA polymerase 2 for transcription of different class 2 genes . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Using a reconstituted in vitro system comprised of purified RNA polymerase 2 , TFIIB , TFIID , TFIIE , and TFIIF , we found that TFIIG was essential for specific initiation from all class 2 genes tested . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Consistent with these characteristics , HTLV 1 transcription activity was reconstituted in vitro by using TATA binding protein , TFIIA , recombinant TFIIB , TFIIE , and TFIIF , TFIIH , and pol 2 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The immunoprecipitate includes RNA polymerase 2 , TFIID , TFIIB , TFIIH , TFIIF , and TFIIE , but is devoid of transcriptional activator proteins , such as HNF 1 , HNF 4 , and C / EBP alpha . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
During this transition , TFIID remains promoter bound whereas TFIIB , TFIIE , TFIIF , and TFIIH are released . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In a reconstituted in vitro transcription assay , Tax 1 transactivation was dependent upon basal transcription factors TFIIB , TFIIF , Pol 2 , TFIID , and TFIIA . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Identification of the gene ( SSU71 / TFG1 ) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae . ^^^ Taken together , these data demonstrate a functional interaction between TFIIB and the large subunit of TFIIF and that this interaction can affect start site selection in vivo . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
TFIIB inhibits the stimulatory activity of TFIIF but has no effect on CTD phosphatase activity in the absence of TFIIF . ^^^ The potential importance of the docking site on RNAP 2 and the effect of TFIIF and TFIIB in regulating the dephosphorylation of RNAP 2 at specific times in the transcription cycle are discussed . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
With the exception of the AdML promoter , formation of stable preinitiation intermediates at each of the promoters tested was found to be strongly dependent on RNA polymerase 2 , holoTFIID , and TFIIB and was stimulated by TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
TFIIF significantly enhanced the initiation of transcription by the bacterial RNA polymerase while other general transcription factors , TATA binding protein , TFIIB , and TFIIE , did not . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
RAP 30 , the small subunit of TFIIF , binds to TFIIB and RNA polymerase 2 and recruits RNA polymerase 2 to a preinitiation complex containing TBP and TFIIB . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
TFIIE is a heterodimer composed of 56 kDa ( p 56 ) and 34 kDa ( p 34 ) subunits and functions in concert with the TATA factor , TFIIB , TFIIF , and TFIIH to promote formation of the RNA polymerase 2 preinitiation complex . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Abortive initiation assays revealed that TATA binding protein , transcription factors TFIIB and TFIIF , and pol 2 were necessary and sufficient to form functional initiation complexes on both linear and supercoiled templates . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In this study accurate initiation of transcription was reconstituted with purified , Escherichia coli synthesized TFIIB , TBP ( the TATA box binding polypeptide of the TFIID complex ) , and the 30 kD subunit of TFIIF ( also known as RAP 30 ) , along with purified , native RNA polymerase 2 from Drosophila embryos , calf thymus , or HeLa cells . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
TFIIB was found to interact with the TATA binding protein , the small subunit of TFIIF , and RNA polymerase 2 . ^^^ Using the gel mobility shift assay , it was found that the amino terminus of TFIIB was necessary for the formation of complexes containing RNA polymerase 2 and TFIIF , whereas the carboxy terminal domain , which is composed of two imperfect direct repeats and includes a putative amphipathic alpha helix , was sufficient for the formation of complexes containing the TATA binding protein and TFIIB ( DB complex ) . ^^^ The interaction with the small subunit of TFIIF was mapped to the amino terminus of TFIIB , which includes a zinc finger . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
First , we observe that synthesis of the first phosphodiester bond of transcripts initiated at the adenovirus 2 major late promoter depends strongly on ATP , TFIIE , and TFIIH in a transcription system reconstituted with RNA polymerase 2 , TFIIH , and recombinant TBP , TFIIB , TFIIE , and TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In the same assay , ICP 4 was unable to interact with the basal transcription factors , TFIIB , TFIIE , TFIIF , and TFIIH and RNA polymerase 2 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Here we report that Gal11p stimulates basal transcription in a reconstituted transcription system composed of recombinant or highly purified transcription factors , TFIIB , TFIIE , TFIIF , TFIIH , and TATA box binding protein and core RNA polymerase 2 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The eluted RAP fraction did not contain the holoenzyme components Srb 2 , 4 , 5 + 6p , Gal11p , or Sug1p , but did include the known transcription factors TFIIB and TFIIS and the three subunits of yeast TFIIF ( Ssu71p / Tfg1p , Tfg2p , and Anc1p / Tfg3p ) . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The Cdc73p Paf1p RNA polymerase 2 containing complex also includes Gal11p , and the general initiation factors TFIIB and TFIIF , but lacks TBP , TFIIH , and transcription elongation factor TFIIS as well as the Srbps . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Finally , when using TBP mutants defective on multiple interaction surfaces , we observed synthetic lethal effects between mutations on the TFIIA and TFIIB interfaces but found that mutations implicated in association with polymerase 2 and TFIIF did not have significant effects in vivo . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Initiation factors , TFIIB , TFIIE beta , and TFIIF beta as well as the TATA box binding protein ( TBP ) were found to interact with BTEB 2 when analyzed by in vitro binding experiments , although these interactions could not be attributed to the proline rich domain . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
A complex containing TBP , TFIIB , TFIIF , and RNAPII ( DBPolF complex ) is capable of forming on the promoter in an Inr dependent manner . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
With the availability of the general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , it is now possible to investigate aspects of the mechanism of eukaryotic messenger RNA synthesis in purified , reconstituted RNA polymerase 2 transcription systems . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Our results indicate that hATF 4 interacts with several general transcription factors : TATA binding protein , TFIIB , and the RAP 30 subunit of TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
This holoenzyme contained nearstoichiometric amounts of all the general transcription factors , TFIIB , TFIID ( TBP + TAFIIs ) , TFIIE , TFIIF , and TFIIH , required to accurately initiate transcription in vitro at the adenovirus major late promoter . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The core promoter is the site for assembly of the transcription preinitiation complex , which includes RNA pol 2 and the general transcription fctors TBP , TFIIB , TFIIE , TFIIF , and TFIIH . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In this study , we noted that the extent of activation by ICP 4 in vitro was highly dependent on the purity of TFIID when recombinant TFIIB , TFIIE , and TFIIF were used as sources of these factors . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
By exploiting adenovirus 2 major late promoter derivatives that contain premelted transcriptional start sites and do not require TFIIF , TFIIE , or TFIIH for transcription initiation , we observe ( 1 ) that Elongin is capable of strongly stimulating the rate of synthesis of trinucleotide transcripts by a subcomplex of RNA polymerase 2 , TBP , and TFIIB and ( 2 ) that the ability of Elongin to stimulate synthesis of these short transcripts is substantially reduced by addition of TFIIF to transcription reactions . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The DNA wrapping model describes specific roles for general RNA polymerase 2 transcription factors ( TATA binding protein [ TBP ] , TFIIB , TFIIF , TFIIE , and TFIIH ) , provides a plausible explanation for preinitiation complex isomerization , suggests mechanisms underlying the synergy between transcriptional activators , and suggests an unforseen role for TBP associating factors in transcription . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In initiation , TFIIF induces promoter DNA to wrap almost a full turn around RNA polymerase 2 in a complex that includes the general transcription factors TATA binding protein , TFIIB , and TFIIE . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Our results show that a system consisting of purified recombinant TFIIA , TFIIB , TFIIE , TFIIF , CREB , and Tax , along with highly purified RNA polymerase 2 , affinity purified epitope tagged TFIID , and semipurified TFIIH , supports basal transcription of the HTLV 1 promoter but is not responsive to Tax . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
These studies led to the discovery of the class of general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , which function in intimate association with RNA polymerase 2 and are required for selective binding of polymerase to its promoters , formation of the open complex , and synthesis of the first few phosphodiester bonds of nascent transcripts . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
TFIIB interacts with a promoter complex containing the TATA binding protein ( TBP ) to facilitate interaction with RNA polymerase 2 ( RNA pol 2 ) and the associated transcription factor IIF ( TFIIF ) . ^^^ TFIIB contains a zinc binding motif near the N terminus that is directly involved in the interaction with RNA pol II / TFIIF and plays a crucial role in selecting the transcription initiation site . ^^^ The zinc ribbon structure of TFIIB , in conjunction with the biochemical analyses , suggests that residues on the beta sheet are involved in the interaction with RNA pol II / TFIIF , while the zinc binding site may increase the stability of the beta sheet . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Interestingly , multiple basal transcription factors for RNA polymerase 2 , including TBP , TFIIB , TFIIH , and TFIIF , were found to be associated with MSS 1 . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Using order of addition and template challenge assays performed in a human cell free transcription system reconstituted with recombinant general transcription factors ( TFIIB , TBP , TFIIE , TFIIF ) , a recombinant general cofactor ( PC 4 ) , and highly purified epitope tagged multiprotein complexes ( TFIID , TFIIH , pol 2 ) , we demonstrate that when TBP is used as the TATA binding factor transcriptional activators such as Gal 4 VP16 and human papillomavirus E 2 mainly function by facilitating pol 2 entry to the promoter region . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
To address this , we used formaldehyde crosslinked chromatin immunoprecipitation ( 10 ChIP ) to map TATA binding protein ( TBP ) , transcription initiation factor IIB ( TFIIB ) and IIF ( TFIIF ) , and dHDAC 1 ( RPD 3 ) across several Drosophila promoter regions . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
A mouse in vitro transcription system reconstituted from highly purified RNA polymerase 2 , TFIIH and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^ Here we describe cloning of the mouse cDNAs encoding TFIIB and the small and large TFIIE and TFIIF subunits . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
This model stipulates that wrapping of the promoter DNA around RNAPII and the general initiation factors TBP , TFIIB , TFIIE , TFIIF and TFIIH induces a torsional strain in the DNA double helix that facilitates strand separation and open complex formation . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The measurements showed comparable amounts of RNA polymerase 2 , TFIIE , and TFIIF , lower levels of TBP and TFIIB , and still lower levels of Mediator and TFIIH . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Both recombinant and cellular TFIIB can autoacetylate , markedly stabilizing the interaction between TFIIB and transcription factor TFIIF and activating transcription in vitro and in cells . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Previous studies have shown that transcription factors IIB ( TFIIB ) , IIF ( TFIIF ) , and RNA polymerase 2 ( RNAPII ) play important roles in determining the position of mRNA 5 ' ends in the yeast Saccharomyces cerevisiae . ^^^ Yeast strains containing a deletion of the small , nonessential Rpb 9 subunit of RNAPII exhibit an upstream shift in the positions of mRNA 5 ' ends , whereas mutation of the large subunit of yeast TFIIF ( Tfg 1 ) can suppress downstream shifts that are conferred by mutations in TFIIB . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
We have now purified S . pombe RNA polymerase 2 and its general initiation factors TFIIB , TFIIF , TFIIE , and TFIIH to near homogeneity . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In situ binding assays , immunoprecipitation , and Western blot analyses demonstrated that mGPBP is a nuclear factor that can form complexes with TATA binding protein , TFIIB , TFIIF , RNA polymerase 2 , and P300 / CBP both in vitro and in intact cells . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The N terminal domain ( NTD ) of TFIIB contains a zinc ribbon motif , which is responsible for the recruitment of RNA polymerase 2 and TFIIF to the core promoter region . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Acetylation of TFIIB results in a stronger interaction with transcription factor IIF ( TFIIF ) and activated transcription in vitro . ^^^ In addition , we report for the first time that the RAP 30 subunit of TFIIF is also auto acetylated in the presence of acetyl coenzyme A in a pH dependent manner , similarly to TFIIB . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The trajectory of promoter DNA determined by the C terminal domain of TFIIB traverses sites of interaction with TFIIE , TFIIF , and TFIIH , serving to define their roles in the transcription initiation process . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
RNA polymerase 2 ( RNAPII ) requires a set of general transcription factors TFIIA , TFIIB , TFIID , TFIIE , TFIIF and TFIIH to initiate transcription from a gene promoter in vitro . ^^^ In this report , we describe a reconstituted in vitro transcription system that consists of the following preparations of factors from the yeast Schizosaccharomyces pombe : affinity purified RNAPII , TFIIH , and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Double affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits , the general transcription factors TFIIB and TFIIF , the RNAPII phosphatase Fcp 1 , and a novel 153 kDa polypeptide of unknown function that we named RNAPII associated protein 1 ( RPAP 1 ) . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
We have studied repair and transcription activities at 8 oxoG lesions with a reconstituted transcription system ( RTS ; RNA polymerase 2 , TBP , TFIIA , TFIIB , TFIIE , TFIIF and TFIIH ) , as well as in cellular extracts and in vivo . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
We used site specific protein DNA photo cross linking to define contact points between Saccharomyces cerevisiae RNA polymerase 2 ( RNAP 2 ) and the general transcription factors TBP , TFIIB , and TFIIF on promoter DNA . ^^^ We present three key findings : ( 1 ) the overall pattern of cross link sites is remarkably similar between the yeast and the previously described human system , even though transcription initiates downstream of the DNA TBP TFIIB RNAP 2 TFIIF complex in the S . cerevisiae system ; ( 2 ) the yeast Rpb 7 subunit of RNAP 2 forms strong and reproducible cross links to both strands of promoter DNA ; and ( 3 ) a TFIIB arginine 78 to cysteine replacement ( R78C ) , which shifts start site selection downstream of normal , does not affect TFIIB DNA cross links prior to promoter melting but instead affects downstream TFIIF DNA interactions . ^^^ The novel Rpb 7 DNA contact sites imply that either promoter DNA does not follow a straight path from TATA to the initiation site or the topology of Rpb 7 within the DNA TBP TFIIB RNAP 2 TFIIF complex is different from that defined in the 12 subunit RNAP 2 10 ray structure . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The TFIIF subunit Tfg 1 was found in close proximity to the TFIIB B finger , linker , and core domains , suggesting that these two factors closely cooperate during initiation . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
We have analyzed an interaction of the general transcription complex RNA polymerase 2 proteins ( RNA polymerase 2 , factors TBP , TFIIB , TFIIF , TFIIE and TFIIH ) S . cerevisiae with the oligoribonucleotides . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
We discuss these results within the context of a proposed model regarding the concerted roles of RNAPII , TFIIB , and TFIIF during mRNA 5 ' end formation in S . cerevisiae . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
The ssu 71 alleles of the TFG 1 gene , which encodes the largest subunit of TFIIF , were isolated as suppressors of a TFIIB defect that affects the accuracy of transcription start site selection in the yeast Saccharomyces cerevisiae . ^^^ We propose that the TFIIF dimerization domain is located in proximity to the B finger of TFIIB near the active center of RNAP 2 where the TFIIB TFIIF RNAP 2 interface plays a key role in start site selection . . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
These studies indicate that the hsp 70 promoter is already occupied by TATA binding protein ( TBP ) and several TBP associated factors ( TAFs ) , TFIIB , TFIIF ( RAP 30 ) , TFIIH ( XPB ) , TBP free / TAF containg complex ( GCN 5 and TRRAP ) , and the Mediator complex subunit 13 before heat shock . ^^^ Surprisingly , upon heat shock , there is a marked diminution in the occupancy of TBP , six different TAFs , TFIIB , and TFIIF , whereas there is no change in the occupancy of these factors at ecdysone induced loci under the same conditions . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Also mapped were segments of TFIIB , TFIIE , TFIIF and TFIIH in proximity to promoter DNA . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
In eukaryotes , the core promoter serves as a platform for the assembly of transcription preinitiation complex ( PIC ) that includes TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIH , and RNA polymerase 2 ( pol 2 ) , which function collectively to specify the transcription start site . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
Remarkably , a large number of promoters assemble into partial preinitiation complexes ( partial PICs ) , containing TFIIA , TFIID ( and / or SAGA ) , TFIIB , TFIIE , and TFIIF . ^^^
Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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Interacting proteins: P35269 and Q00403	Pubmed	SVM Score :0.0
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