| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.85244805 |
| Using a yeast two hybrid protein interaction assay , we demonstrate that VDR forms specific protein : protein contacts with the basal transcription factor TFIIB . 0.85244805^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.64330896 |
| In glutathione S transferase fusion based protein protein binding assays we found that VDR specifically binds to TFIIB , a component of the basal complex , and that the interaction requires select domains of each protein . 0.64330896^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.66353341 |
| In addition , the transcription factor TFIIB has been shown to bind VDR but there is currently no evidence that a co repressor or co activator of VDR is also involved . 0.66353341^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These data implicate amino acids Leu 417 and Glu 420 , residing in a putative alpha helical region at the extreme C terminus of hVDR , as critical in the mechanism of 1 , 25 ( OH ) 2D3 stimulated transcription , likely mediating an interaction with a coactivator ( s ) or a component of the basal transcriptional machinery distinct from TFIIB . . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| TFIIB and VDR can also interact directly , and these factors synergize to mediate transactivation . ^^^ Our results suggest that YY 1 regulates vitamin D enhancement of osteocalcin gene transcription in vivo by interfering with the interactions of the VDR with both the VDRE and TFIIB . . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| The interaction of the vitamin D receptor ( VDR ) with transcription factor IIB ( TFIIB ) represents a potential physical link between the VDR DNA complex and the transcription preinitiation complex . ^^^ However , the functional relevance of the VDR TFIIB interaction in vitamin D mediated transcription is not well understood . ^^^ In the present study , we used site directed mutagenesis to demonstrate that the structural integrity of the amino terminal zinc finger of TFIIB is essential for VDR TFIIB complex formation . ^^^ Altering the putative zinc coordinating residues ( C 15 , C 34 , C 37 , or H 18 ) to serines abolished TFIIB interaction with the VDR as assessed in a yeast two hybrid system and by in vitro protein interaction assays . ^^^ A mutant N terminal domain [ N TFIIB ( C34S : C37S ) ] that does not interact with VDR had no impact on 1 , 25 ( OH ) 2D3 induced transcription . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Additional probing of protein protein interactions has revealed that VDR also binds to basal transcription factor IIB ( TFIIB ) and , in the presence of 1 , 25 ( OH ) 2D3 , an RXR VDR TFIIB ternary complex can be created in solution . ^^^ Because the AF 2 domains participate neither in VDR RXR heterodimerization nor in TFIIB association , it is hypothesized that they contact , in a ligand dependent fashion , transcriptional coactivators such as those of the steroid receptor coactivator family , constituting yet a third protein protein interaction for VDR . ^^^ The above events , including bridging by coactivators to the TATA binding protein and associated factors , may position VDR such that it is able to attract TFIIB and the balance of the RNA polymerase 2 transcription machinery , culminating in repeated transcriptional initiation of VDRE containing , vitamin D target genes . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Because the f VDR genotype has been associated with lower bone mineral density in diverse populations , one factor contributing to a genetic predisposition to osteoporosis may be the F / f polymorphism that dictates VDR isoforms with differential TFIIB interaction . . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| Thus , CBP may alleviate YY 1 mediated repression , in part , by preventing YY 1 from binding to TFIIB , which is required for VDR mediated transcription . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| These studies extend our previous studies related to factors that affect vitamin D receptor ( VDR ) mediated 24 ( OH ) ase transcription ( YY 1 , TFIIB , CBP ) and the effect of signaling pathways on 24 ( OH ) ase transcription and cofactor recruitment . . ^^^ |
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| Interacting proteins: P11473 and Q00403 |
Pubmed |
SVM Score :0.0 |
| SKIP / NCoA 62 , the basal transcription factor TFIIB , and RXR all interacted with VDR helix H 10 mutants at reduced levels compared with wild type in the absence of ligand and exhibited different degrees of increased interaction upon ligand addition . ^^^ |
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